CN113616546A

CN113616546A - Peach resin polysaccharide gum capable of brightening skin, application and extraction preparation method thereof

Info

Publication number: CN113616546A
Application number: CN202110795385.5A
Authority: CN
Inventors: 李晓敏; 蔡建平; 赵鑫
Original assignee: Shanghai Jiaxin Biotechnology Co ltd
Current assignee: Shanghai Jiaxin Biotechnology Co ltd
Priority date: 2021-07-14
Filing date: 2021-07-14
Publication date: 2021-11-09
Anticipated expiration: 2041-07-14
Also published as: CN113616546B

Abstract

The invention discloses a peach resin polysaccharide gum capable of brightening skin, an application and an extraction preparation method thereof. The invention provides a peach resin polysaccharide gum capable of brightening skin, which comprises the following components in parts by weight: 59.95-73 parts of peach resin extract; 0.05-1 part of dendrobium officinale extract; 10-15 parts of pullulanase polysaccharide; 10-15 parts of tremella extract; and 1-10 parts of trehalose. The skin care product is prepared from the pure natural plant extracts, has comprehensive improvement and conditioning effects on skin problems, is mild and safe when being used as a skin care product, can be used as an effect additive to prepare various skin care products, and further realizes the comprehensiveness and the comprehensiveness of the effects of the skin care products.

Description

Peach resin polysaccharide gum capable of brightening skin, application and extraction preparation method thereof

Technical Field

The invention relates to the technical field of daily chemical industry, in particular to a plant extract mixture with moisturizing and anti-inflammatory effects.

Background

Dry skin, reduced elasticity, not only affects the appearance of the skin, but also causes subjective discomfort to the consumer and may cause deterioration of certain skin conditions. The hydration state and the loss degree of the skin are directly related to the softness and elasticity of the skin, and the moisture degree of the skin can be maintained to enhance the elasticity of the skin, ensure the normal metabolism of the skin and reduce the generation of wrinkles and color spots. Generally, the moisture content of the stratum corneum of healthy skin is kept between 10% and 20%. The moisturizing effect is the most basic function of the skin care cosmetics, and how to improve the moisturizing effect of the cosmetics has important significance on research and development and medical clinical application of the cosmetics.

Peach gum is a polysaccharide natural substance, has moisturizing effect and thickening effect, but is not easy to completely dissolve, and currently, the preparation of peach gum mainly comprises a hydrolysis method, an enzyme extraction method and the like, however, the hydrolysis method has high cost and is not beneficial to industrial production; the enzyme extraction technique has some disadvantages that the optimum temperature and optimum pH of the enzyme tend to be in a small range. In order to increase the activity of the enzyme to the maximum, the reaction temperature and pH of the enzyme must be very strictly controlled, and even small fluctuations in the reaction conditions may greatly reduce the activity of the enzyme, so that the demand for experimental equipment is high.

Disclosure of Invention

The invention aims to overcome the defects in the prior art and provides a peach resin polysaccharide gum with bright skin, application and an extraction and preparation method thereof.

The purpose of the invention is realized by the following technical scheme:

the invention provides a peach resin polysaccharide gum capable of brightening skin, which comprises the following components in percentage by weight:

preferably, the peach resin polysaccharide gum further comprises water, glycerol, 1, 2-hexanediol, and phenoxyethanol.

Preferably, the peach resin is produced from Guizhou water.

The dendrobium officinale adopts dendrobium officinale with a production place of Huoshan.

In a second aspect, the present invention provides a method for preparing the above peach resin polysaccharide gum, comprising the steps of:

A. respectively crushing and sieving peach resin, dendrobium officinale and the mixture, adding ethanol, and performing reflux extraction at 50-100 ℃ for 4-8 hours, wherein the weight-volume ratio of feed liquid is 1: 5-50;

B. filtering the extractive solution, and vacuum concentrating to remove alcohol smell;

C. b, adding water into the concentrated solution prepared in the step B for dispersion, loading the concentrated solution onto a macroporous resin column or a polyamide resin column, eluting by using water with the volume of 3-5 times of the column volume, eluting by using an ethanol water solution with the volume fraction of 30% -45% and the volume of 4-6 times of the column volume, eluting by using an ethanol water solution with the volume fraction of 50% -75% and the volume fraction of 4-6 times of the column volume, eluting by using an ethanol water solution with the volume fraction of 85% -95%;

D. mixing the eluates, and concentrating to 1/3-1/6 of the original weight to obtain extracts of each component;

E. mixing the extracts according to the weight percentage to obtain the peach resin polysaccharide gum.

Preferably, in the step a, the ethanol is absolute ethanol.

Preferably, in the step B, the degree of vacuum is less than 0.1 MPa.

In a third aspect, the invention provides the use of the peach resin polysaccharide gum for preparing skin care cosmetics.

Preferably, the skin care cosmetic is selected from: moisturizing skin care cosmetic or antioxidant aging cosmetic.

Preferably, the skin care cosmetic is in the form of a lotion, cream, milky lotion, pack, gel, essence, face toilet, and body wash.

Preferably, the peach resin polysaccharide gum accounts for 1-10% of the total weight of the skin care cosmetic.

Compared with the prior art, the invention has the following beneficial effects:

1. the added natural components are safe, mild and effective, and have the functions of diminishing inflammation and conditioning the phenomena of molting, itching, eczema, whelk, pustule and the like easily appearing on the skin.

2. Through the compound use, the anti-inflammatory, moisturizing and nutritional effects are fully exerted, and the skin-moistening cream has the auxiliary effects of nourishing the skin, diminishing inflammation, resisting oxidation and the like, so that the effect of comprehensively improving the problem of dry skin is achieved.

3. Not only has the function of relieving existing symptoms, but also has the function of preventing or delaying adverse conditions of dry skin.

Drawings

Other features, objects and advantages of the invention will become more apparent upon reading of the detailed description of non-limiting embodiments with reference to the following drawings:

fig. 1 shows that peach resin polysaccharide gum is prepared into cream, peach resin polysaccharide with the mass percentage of 20% in the cream is subjected to skin spot test, wherein (a) is the condition of skin spot position for 24 h; (b) the skin patch test is 48 h;

FIG. 2 shows anti-inflammatory test using peach gum with 0% by weight of polysaccharide gum, wherein (a) is skin condition at 0min, (b) is skin condition at 10min, and (c) is skin condition at 30 min;

FIG. 3 shows anti-inflammatory test using peach gum with 1% by weight of polysaccharide gum, wherein (a) is skin condition at 0min, (b) is skin condition at 10min, and (c) is skin condition at 30 min;

FIG. 4 shows anti-inflammatory test with addition of 3 wt% peach gum, wherein (a) is skin condition at 0min, (b) is skin condition at 10min, and (c) is skin condition at 30 min;

FIG. 5 shows anti-inflammatory test with 5 wt% peach gum, wherein (a) is skin condition at 0min, (b) is skin condition at 10min, and (c) is skin condition at 30 min;

FIG. 6 shows anti-inflammatory test with 10 wt% peach gum, wherein (a) is skin condition at 0min, (b) is skin condition at 10min, and (c) is skin condition at 30 min;

FIG. 7 shows anti-inflammatory test with addition of 20 wt% peach gum, wherein (a) is skin condition at 0min, (b) is skin condition at 10min, and (c) is skin condition at 30 min;

FIG. 8 shows the results of moisture retention for various concentrations of peach gum after various times;

fig. 9 shows the results after the transdermal moisturizing test of peach resin polysaccharide gum at various concentrations.

Detailed Description

The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that variations and modifications can be made by persons skilled in the art without departing from the spirit of the invention. All falling within the scope of the present invention.

In the present invention, the term "peach resin extract" refers to an extract containing an effective ingredient extracted from peach resin by any suitable means. The term "extract of dendrobium officinale" refers to an extract containing an active ingredient extracted from dendrobium officinale by any suitable means. The term "tremella root extract" refers to an extract containing active ingredients extracted from tremella by any suitable means.

The term "cosmetic" means to be spread on any part of the surface of the human body by spreading, spraying or other similar methods, such as: skin, hair, nails, lips and teeth, etc., for the purpose of cleaning, caring, beautifying, modifying and changing the appearance, or correcting human body odor, and maintaining good state. According to a preferred embodiment of the invention, the cosmetic product comprises: moisturizing cosmetics, anti-inflammatory cosmetics with moisturizing effect or anti-aging cosmetics with moisturizing effect.

Example 1

The embodiment relates to a peach resin polysaccharide gum for brightening skin, which comprises the following components in percentage by weight; peach resin extract: 73 parts; the dendrobium officinale extract: 1 part; pullulanase: 10 parts of (A); white fungus extract: 10 parts of (A); 5 parts of trehalose; 0.1 part of 1, 2-hexanediol and 0.1 part of phenoxyethanol, wherein the weight ratio of glycerol: 0.8 part.

The preparation method comprises the following steps:

E. mixing the extracts of the components according to the weight parts to obtain the peach resin polysaccharide gum.

Example 2

The embodiment relates to a peach resin polysaccharide gum for brightening skin, which comprises the following components in percentage by weight; peach resin extract: 59.95 parts; the dendrobium officinale extract: 0.05 part; pullulanase: 15 parts of (1); white fungus extract: 15 parts of (1); 9 parts of trehalose; 0.1 part of 1, 2-hexanediol and 0.1 part of phenoxyethanol, wherein the weight ratio of glycerol: 0.8 part.

The preparation method comprises the following steps:

Example 3

The embodiment relates to a peach resin polysaccharide gum for brightening skin, which comprises the following components in percentage by weight; peach resin extract: 64.92 parts; the dendrobium officinale extract: 0.08 part; pullulanase: 13 parts; white fungus extract: 13 parts; 8 parts of trehalose; 0.1 part of 1, 2-hexanediol and 0.1 part of phenoxyethanol, wherein the weight ratio of glycerol: 0.8 part.

The preparation method comprises the following steps:

Example 4 Spot Patch test

The experimental method comprises the following steps: preparing cream containing 20% of peach gum extract, performing skin patch test, and observing skin patch parts at 24h and 48h after patch application, wherein the specific result is shown in fig. 1.

Fig. 1 is a process for preparing peach resin polysaccharide gum of the present invention into cream, wherein peach resin polysaccharide gum with 20% mass percentage is prepared in cream (the peach resin polysaccharide gum is prepared by the preparation method of example 1, but it is conceivable that the preparation methods of other embodiments can be used for obtaining peach resin polysaccharide gum and has the same efficacy), and skin patch test is performed, wherein (a) is 24h skin patch site condition; (b) the skin patch test is 48 h.

According to the skin reaction grading standard of the skin closed patch experiment shown in the following table 1, it can be seen that when the cream contains 20% by mass, the cream is safe and non-irritant.

Table 1.

Example 5 anti-inflammatory assay

The experimental method comprises the following steps: testing the allergic reaction of the skin by using a histamine solution with the mass percent of 4%; fig. 2 to 7 show that the anti-inflammatory test is performed for 0 to 30min by using peach gum solution with different mass percentage, wherein fig. 2 shows that the anti-inflammatory test is performed by adding 0 mass% peach gum, wherein (a) is skin condition at 0min, (b) is skin condition at 10min, and (c) is skin condition at 30 min;

FIG. 7 shows anti-inflammatory test with addition of 20 wt% peach gum, wherein (a) is skin condition at 0min, (b) is skin condition at 10min, and (c) is skin condition at 30 min.

As can be seen from fig. 2 to 7, the peach resin polysaccharide gum solution has no anti-inflammatory effect at each mass percentage.

Example 6, moisture retention test:

firstly, in-vitro moisture retention test:

the experimental method comprises the following steps:

marking the beaker, placing the beaker in a drying oven at 105 ℃ for drying to constant weight, weighing the beaker after the beaker is cooled to room temperature, and counting the weight as m₁；

Weighing appropriate amount of peach resin polysaccharide gum with various concentrations, and allowing the liquid level to just submerge the bottom of the bottle, and recording as m₂(weigh flask + sample);

placing the beaker with the sample in a humidistat (supersaturated solution of calcium chloride) with humidity of about 30% for 1h, 3h, 5h, 7h,Weighing after 24h, wherein the weight of the weighing at that time is m₃。

Moisture retention rate (m)₃-m₁)/(m_2-m₁) X 100%, and a higher value of the moisturizing ratio indicates better moisturizing performance of the corresponding product.

The test result image is shown in fig. 8, in which a curve I represents the moisturizing rate of a peach gum solution with a mass percentage of 0%, a curve II represents the moisturizing rate of a peach gum solution with a mass percentage of 1%, a curve III represents the moisturizing rate of a peach gum solution with a mass percentage of 5%, a curve IV represents the moisturizing rate of a peach gum solution with a mass percentage of 25%, a curve V represents the moisturizing rate of a peach gum solution with a mass percentage of 10%, and a curve VI represents the moisturizing rate of a peach gum solution with a mass percentage of 50%.

Second, percutaneous moisture retention test

The experimental method comprises the following steps: firstly measuring the moisture content of the skin in the arm without the cream, then respectively taking 1g of the cream added with the peach resin polysaccharide gum with each concentration, smearing the cream in the arm, respectively measuring the moisture content of the skin after 1h, 2h and 4h, and finally calculating the change rate of the moisture content of the skin.

The final test results are shown in fig. 9, wherein the column I is a blank substrate, the column II is a cream with an additive amount of 2% by mass, and the column III is a cream with an additive amount of 5% by mass. As can be seen from FIG. 9, the peach resin polysaccharide gum can significantly improve the moisture retention rate and the skin moisture content.

The foregoing description of specific embodiments of the present invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes and modifications may be made by one skilled in the art within the scope of the appended claims without departing from the spirit of the invention.

Claims

1. A peach resin polysaccharide gum for brightening skin is characterized by comprising the following components in parts by weight:

2. a peach resin polysaccharide gum as claimed in claim 1, further comprising water, glycerol, 1, 2-hexanediol and phenoxyethanol.

3. A peach resin polysaccharide gum as claimed in claim 1, wherein the peach resin is produced from Guizhou water of origin.

4. A method for preparing the peach resin polysaccharide gum as claimed in any one of claims 1 to 3, comprising the steps of:

5. The production method according to claim 4, wherein in the step A, the ethanol is anhydrous ethanol.

6. The production method according to claim 4, wherein in the step B, the degree of vacuum is less than 0.1 MPa.

7. Use of a peach resin polysaccharide gum according to any one of claims 1 to 3, wherein the peach resin polysaccharide gum is used for preparing skin care cosmetics.

8. Use according to claim 7, characterized in that the skin care cosmetic is selected from: moisturizing skin care cosmetic or antioxidant aging cosmetic.

9. Use according to claim 7, characterized in that the skin-care cosmetic is in the form of lotions, creams, lotions, masks, gels, essences, facial washes and shower gels.

10. Use according to claim 7, wherein the peach resin polysaccharide gum represents 1% to 10% of the total weight of the skin care cosmetic.