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CN113304087B - Body lotion - Google Patents

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Publication number
CN113304087B
CN113304087B CN202110576889.8A CN202110576889A CN113304087B CN 113304087 B CN113304087 B CN 113304087B CN 202110576889 A CN202110576889 A CN 202110576889A CN 113304087 B CN113304087 B CN 113304087B
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extract
parts
folic acid
betaine
litchi
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CN113304087A (en
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岳玉荣
韩雅慧
易斌
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Jianchangbang Pharmacy Co ltd
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Jianchangbang Pharmacy Co ltd
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783Angiosperms [Magnoliophyta]
    • A61K8/9794Liliopsida [monocotyledons]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/34Alcohols
    • A61K8/345Alcohols containing more than one hydroxy group
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/40Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
    • A61K8/44Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/40Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
    • A61K8/44Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
    • A61K8/442Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof substituted by amido group(s)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/67Vitamins
    • A61K8/673Vitamin B group
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783Angiosperms [Magnoliophyta]
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04Antibacterial agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • A61P37/02Immunomodulators
    • A61P37/04Immunostimulants
    • AHUMAN NECESSITIES
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    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • A61P37/08Antiallergic agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/005Antimicrobial preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/02Preparations for care of the skin for chemically bleaching or whitening the skin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59Mixtures
    • A61K2800/592Mixtures of compounds complementing their respective functions
    • A61K2800/5922At least two compounds being classified in the same subclass of A61K8/18
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    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K2800/82Preparation or application process involves sonication or ultrasonication
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine

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  • Cosmetics (AREA)

Abstract

The invention belongs to the technical field of skin care products, and particularly relates to body milk. The body lotion comprises the following components: glycerol, betaine, lauramidopropyl betaine, folic acid compounds, bamboo leaf flavone, litchi extract, cistanche extract and honeysuckle extract. The body milk can supplement folic acid, lighten striae gravidarum and improve the immunity to the external environment. Meanwhile, the body milk has low production cost and simple preparation process, and is suitable for industrial production.

Description

Body lotion
Technical Field
The invention belongs to the technical field of skin care products, and particularly relates to body milk.
Background
Body milk is a cosmetic emulsion for use on the body. The body lotion has the common effects of moisturizing, whitening and moistening the whole body, and is divided into multiple functions of smoothing the whole body, preventing sunburn, reducing weight, repairing and maintaining, and making the body attractive, thereby improving the personal charm and attracting the attention of the opposite sense. Body milk can be subdivided into products required for many parts such as face, neck, upper limbs, hands, body, buttocks, abdomen, lower limbs, feet, etc. according to body parts; according to the subdivision of the product efficacy, a plurality of body care products for moisturizing, whitening, slimming, body-building, body-perfuming, cutin removal and the like can be provided; the components can be further subdivided according to the flavor and raw materials, such as essence and spice blended products, purely natural plant flavor products, essential oil additive products and the like.
The female has larger hormonal change in gestation period, and the abdomen bulge causes the elastic fiber and the collagen fiber of the skin to be damaged or broken, so that the abdomen skin becomes thinner and thinner, and then, pink or purple wavy patterns with different widths and different lengths can appear. After delivery of the woman, these figures fade away leaving behind white or silvery shiny scar lines, which are what we commonly call striae of pregnancy. And once striae gravidarum is formed, the striae gravidarum basically cannot repair and disappear by oneself. Therefore, many women are deeply afflicted with children after the children are born, and can all want to eliminate the trouble.
However, the body milk on the market is not friendly to pregnant and lying-in women, and is easy to stimulate the skin with delicate abdomen, so that there is a need to develop a body milk suitable for pregnant and lying-in women, which can lighten striae gravidarum.
Chinese patent application CN108451876a discloses a method for preparing body milk for striae gravidarum removal, which comprises the following steps: 20-30 parts of chitin, 30-40 parts of borage oil, 2-8 parts of ethylene glycol, 2-9 parts of almond oil, 10-20 parts of poria cocos extract, 2-8 parts of centella asiatica extract, 5-15 parts of alfalfa extract, 4-6 parts of stabilizer, 3-6 parts of emulsifier, 10-20 parts of emollient, 3-7 parts of lawn pennywort extract, 5-10 parts of hydrogenated soybean lecithin, 3-8 parts of saccharic acid, 12-18 parts of cacumen biotae extract, 11-14 parts of angelica extract, 3-8 parts of allantoin, 5-10 parts of amniotic fluid and 5-10 parts of oat polypeptide. The product has the advantages of pure nature, no hormone, convenient operation, quick absorption, skin tendering and lubrication, skin contraction and tightening, and the components of the traditional Chinese medicines complement each other, so that striae gravidarum is reduced, the treatment effect is obvious, and no toxic or side effect is caused. However, the numerous components in this technology add complexity and cost to the preparation and interactions between the components can make the system unstable.
The invention aims to research the body milk which has no side effect and simple preparation process.
Disclosure of Invention
In order to overcome the technical problems, the invention provides body milk. The body milk can supplement folic acid, lighten striae gravidarum and improve the immunity to the external environment. Meanwhile, the body milk has low production cost and simple preparation process, and is suitable for industrial production.
In order to achieve the above object, the technical scheme provided by the invention is as follows:
a body lotion comprising the following components: glycerol, betaine, lauramidopropyl betaine, folic acid compounds, bamboo leaf flavone, litchi extract, cistanche extract and honeysuckle extract.
Preferably, the body milk comprises the following components in parts by weight:
15-40 parts of glycerin, 15-30 parts of betaine, 10-20 parts of lauramidopropyl betaine, 0.0001-0.005 part of folic acid compounds, 1-5 parts of bamboo leaf flavone, 2-8 parts of litchi extract, 1-3 parts of cistanche extract and 1-3 parts of honeysuckle extract.
Preferably, the body milk comprises the following components in parts by weight:
30 parts of glycerin, 25 parts of betaine, 15 parts of lauramidopropyl betaine, 0.003 part of folic acid compounds, 2 parts of bamboo leaf flavonoids, 5 parts of litchi extract, 2 parts of cistanche extract and 2 parts of honeysuckle extract.
Preferably, the folic acid compounds are one or more of folic acid, leucovorin, L-methyl folic acid, folic acid medicinal salt, folic acid or folic acid medicinal salt active metabolite and substances capable of being metabolized and/or generating folic acid in vivo.
Preferably, the preparation method of the bamboo leaf flavone comprises the following steps:
steaming folium Bambusae, adding cellulase and pectase, performing enzymolysis at 30-45deg.C, filtering, and collecting filtrate
Figure BDA0003084728470000021
Adsorbing with XAD-7 macroporous resin, eluting with 45-75% ethanol water solution, collecting eluate, and drying to obtain bamboo leaf flavone.
Preferably, the dosage of the cellulase is 0.5-1% of the mass of the bamboo leaves;
preferably, the pectase is used in an amount of 1.0-1.5% of the mass of the bamboo leaves.
Preferably, the preparation method of the litchi extract comprises the following steps:
pulverizing fructus litchi pulp, filtering, sterilizing fructus litchi juice, inoculating yeast and lactobacillus, fermenting, separating and purifying the fermentation product, and filtering to obtain fructus litchi extract with molecular weight less than 5000 Da.
Preferably, the weight ratio of the saccharomycetes to the lactic acid bacteria is 1:1-4.
Preferably, the inoculation amount of the mixed strain is that the concentration of the strain after inoculation is 10 5 -10 6 CFU/ml。
Preferably, the preparation method of the cistanche extract comprises the following steps:
a: pulverizing herba cistanches, reflux-extracting with 70-80% ethanol water solution, concentrating the extractive solution, and drying to obtain extract;
b: dissolving the extract in water, extracting with ethyl acetate or n-butanol for 2-3 times, filtering under reduced pressure, mixing filtrates, concentrating at high temperature, purifying, vacuum drying, pulverizing, sterilizing, and sieving with 100-120 mesh sieve to obtain herba cistanches extract; taking a cistanche extract sample, detecting by HPLC, wherein the echinacoside content in the cistanche extract reaches 60-80% to be qualified.
Preferably, the number of times of reflux extraction in the step A is 2-3, and each time of extraction is 1.5-2 hours.
Preferably, the addition amount of the ethanol aqueous solution in the step A is 2-8 times of the mass of cistanche deserticola.
Preferably, the relative density of the extract is 1.05-1.15.
Preferably, the preparation method of the honeysuckle extract comprises the following steps: weighing flos Lonicerae, adding into ethanol water solution, soaking under negative pressure, decocting under heating, and filtering; decolorizing the obtained filtrate, and concentrating to obtain flos Lonicerae extract.
Preferably, the mass concentration of the ethanol aqueous solution is 45-75%, and the dosage is 4-8 times of the mass of the honeysuckle.
Preferably, the negative pressure condition is a pressure of 20-60kpa.
Another object of the present invention is to provide a method for preparing the body milk, comprising the steps of:
(1) Placing glycerol, betaine, lauramidopropyl betaine, folic acid compounds, bamboo leaf flavone, litchi extract, cistanche extract and honeysuckle extract in a reactor, and performing ultrasonic treatment and stirring dispersion to obtain a dispersion;
(2) And (3) transferring the dispersion into a preheated homogenizing emulsifying pot, heating, homogenizing and emulsifying, and naturally cooling to normal temperature.
Preferably, in the step (1), the ultrasonic frequency is 20-40KHz, the stirring dispersion speed is 5000-6000r/min, and the dispersion time is 35-55min.
Preferably, in the step (2), the preheating temperature is 60-70 ℃; the temperature during emulsification is 75-80 ℃; the homogenization and emulsification are carried out for 2-4 minutes.
Compared with the prior art, the invention has the technical advantages that:
(1) The raw materials adopted by the body milk provided by the invention are mainly substances rich in moisturizing and moisturizing components, so that folic acid can be supplemented, striae gravidarum can be desalted, and the immunity to the external environment can be improved.
(2) The body lotion provided by the invention has the advantages of low production cost and simple preparation process, and is suitable for industrial production.
(3) The added bamboo leaf flavone, litchi extract, cistanche extract and honeysuckle extract have good synergistic effect, and can effectively promote the moisturizing, antibacterial, anti-inflammatory and antiallergic effects of body milk.
Detailed Description
The present invention will be described by way of specific examples, to facilitate understanding and grasping of the technical solution of the present invention, but the present invention is not limited thereto. The experimental methods described in the following examples are all conventional methods unless otherwise specified; the reagents and materials, unless otherwise specified, are commercially available.
Example 1
(1) Preparation of bamboo leaf flavone:
taking bamboo leaves, steaming with steamAdding into 50% ethanol water solution (mass concentration), adding 0.5% cellulase and 1.0% pectase, performing enzymolysis at 30deg.C, filtering, and collecting filtrate
Figure BDA0003084728470000041
Adsorbing with XAD-7 macroporous resin, eluting with 75% ethanol water solution, collecting eluate, and drying to obtain bamboo leaf flavone.
(2) Preparation of litchi extract:
pulverizing fructus litchi pulp, filtering, sterilizing, inoculating yeast and lactobacillus at weight ratio of 1:1 to obtain strain concentration of 10 5 Performing fermentation treatment on CFU/ml, separating and purifying the fermentation product, and filtering to obtain litchi extract with molecular weight less than 5000 Da.
(3) Preparation of cistanche extract:
a: pulverizing herba cistanches, reflux-extracting with 2 times of 80% ethanol water solution for 2 times for 1.5 hr each time, mixing extractive solutions, concentrating, and drying to relative density of 1.05-1.15 (25deg.C) to obtain extract;
b: dissolving the extract in water, extracting with ethyl acetate for 2 times, filtering the extractive solution under reduced pressure, mixing the filtrates, concentrating at high temperature, purifying, vacuum drying, pulverizing, sterilizing, and sieving with 100 mesh sieve to obtain herba cistanches extract; taking a cistanche extract sample, detecting by HPLC, wherein the echinacoside content in the cistanche extract reaches 78.6% to be qualified.
(4) Preparation of honeysuckle extract:
weighing flos Lonicerae, adding into 4 times of 75% ethanol water solution, soaking under 20kpa condition, heating, decocting, and filtering; decolorizing the obtained filtrate, and concentrating to obtain flos Lonicerae extract.
Example 2
(1) Preparation of bamboo leaf flavone:
steaming folium Bambusae, adding into 50% ethanol water solution (mass concentration), adding 1% cellulase and 1.5% pectase, performing enzymolysis at 45deg.C, filtering, and collecting filtrate
Figure BDA0003084728470000051
Adsorbing with XAD-7 macroporous resin, eluting with 45% ethanol water solution, collecting eluate, and drying to obtain bamboo leaf flavone.
(2) Preparation of litchi extract:
pulverizing fructus litchi pulp, filtering, sterilizing, inoculating yeast and lactobacillus at a weight ratio of 1:4 to obtain a strain concentration of 10 6 Performing fermentation treatment on CFU/ml, separating and purifying the fermentation product, and filtering to obtain litchi extract with molecular weight less than 5000 Da.
(3) Preparation of cistanche extract:
a: pulverizing herba cistanches, reflux-extracting with 8 times of 70% ethanol water solution for 3 times for 2 hr each time, mixing extractive solutions, concentrating, and drying to relative density of 1.05-1.15 (25deg.C) to obtain extract;
b: dissolving the extract in water, extracting with n-butanol for 3 times, filtering the extractive solution under reduced pressure, mixing filtrates, concentrating at high temperature, purifying, vacuum drying, pulverizing, sterilizing, and sieving with 120 mesh sieve to obtain herba cistanches extract; taking a cistanche extract sample, detecting by HPLC, wherein the echinacoside content in the cistanche extract reaches 73.2% to be qualified.
(4) Preparation of honeysuckle extract:
weighing flos Lonicerae, adding into 8 times of 45% ethanol water solution, soaking under 60kpa condition, heating, decocting, and filtering; decolorizing the obtained filtrate, and concentrating to obtain flos Lonicerae extract.
Example 3
The body lotion comprises the following components in parts by weight:
30 parts of glycerin, 25 parts of betaine, 15 parts of lauramidopropyl betaine, 0.003 part of folic acid, 2 parts of bamboo leaf flavone, 5 parts of litchi extract, 2 parts of cistanche extract and 2 parts of honeysuckle extract.
The preparation method of the body milk comprises the following steps:
(1) Placing glycerol, betaine, lauramidopropyl betaine, folic acid, bamboo leaf flavone, litchi extract, cistanche extract and honeysuckle extract in a reactor, and stirring and dispersing for 55min at 5000r/min under ultrasonic wave of 20KHz to obtain a dispersion;
(2) Transferring the dispersion into a homogenizing emulsifying pot preheated at 60deg.C, heating to 75deg.C, homogenizing and emulsifying for 2 min, and naturally cooling to normal temperature.
In this example, bamboo leaf flavone, litchi extract, cistanche extract and honeysuckle extract are all the products prepared in example 1.
Example 4
The body lotion comprises the following components in parts by weight:
15 parts of glycerin, 15 parts of betaine, 10 parts of lauramidopropyl betaine, 0.0001 part of L-methyl folic acid, 1 part of bamboo leaf flavone, 2 parts of litchi extract, 1 part of cistanche extract and 1 part of honeysuckle extract.
The preparation method of the body milk comprises the following steps:
(1) Placing glycerol, betaine, lauramidopropyl betaine, L-methyl folic acid, bamboo leaf flavone, litchi extract, cistanche extract and honeysuckle extract in a reactor, and stirring and dispersing for 35min at 40KHz under ultrasonic wave and 6000r/min to obtain a dispersion;
(2) Transferring the dispersion into a homogenizing emulsifying pot preheated at 70deg.C, heating to 80deg.C for homogenizing and emulsifying for 4 min, and naturally cooling to room temperature.
In this example, bamboo leaf flavone, litchi extract, cistanche extract and honeysuckle extract are all the products prepared in example 2.
Example 5
The body lotion comprises the following components in parts by weight:
40 parts of glycerin, 30 parts of betaine, 20 parts of lauramidopropyl betaine, 0.005 part of leucovorin, 5 parts of bamboo leaf flavone, 8 parts of litchi extract, 3 parts of cistanche extract and 3 parts of honeysuckle extract.
The preparation method of the body milk comprises the following steps:
(1) Placing glycerol, betaine, lauramidopropyl betaine, leucovorin, bamboo leaf flavone, litchi extract, cistanche extract and flos Lonicerae extract in a reactor, and stirring and dispersing at 20-40KHz under 5000-6000r/min for 35-55min to obtain dispersion;
(2) Transferring the dispersion into a homogenizing emulsifying pot preheated at 60-70deg.C, heating to 75-80deg.C for homogenizing and emulsifying for 2-4 min, and naturally cooling to room temperature.
In this example, bamboo leaf flavone, litchi extract, cistanche extract and honeysuckle extract are all the products prepared in example 1.
Comparative example 1
Compared with example 3, the difference is only that the bamboo leaf flavone is replaced by the pseudo-ginseng flavone, and the rest operation is unchanged.
The body lotion comprises the following components in parts by weight:
30 parts of glycerin, 25 parts of betaine, 15 parts of lauramidopropyl betaine, 0.003 part of folic acid, 2 parts of pseudo-ginseng flavone, 5 parts of litchi extract, 2 parts of cistanche extract and 2 parts of honeysuckle extract.
The preparation method of the body milk comprises the following steps:
(1) Placing glycerol, betaine, lauramidopropyl betaine, folic acid, notoginseng radix flavone, litchi extract, cistanchis herba extract, and flos Lonicerae extract in a reactor, and stirring and dispersing at 20KHz under 5000r/min for 55min to obtain dispersion;
(2) Step (2) of example 3 was repeated.
Wherein, the preparation of the pseudo-ginseng flavone comprises the following steps: pulverizing Notoginseng radix, adding into 50% ethanol water solution (mass concentration), adding 0.5% cellulase and 1.0% pectase, performing enzymolysis at 30deg.C, filtering, and collecting filtrate
Figure BDA0003084728470000071
Adsorbing with XAD-7 macroporous resin, eluting with 75% ethanol water solution, collecting eluate, and drying to obtain Notoginseng radix flavone.
The sources of the remaining extracts were the same as in example 3.
Comparative example 2
The only difference compared with example 3 is that the preparation method of cistanche extract is different, and the rest operation is unchanged.
Body milk, the composition of which is the same as in example 3; the procedure is as in example 3.
The preparation method of the cistanche extract comprises the following steps:
a: pulverizing herba cistanches, reflux-extracting with 2 times of 80% ethanol water solution for 3 hr, mixing extractive solutions, concentrating, and drying to relative density of 1.05-1.15 to obtain extract;
b: dissolving the extract in water, extracting with ethyl acetate, filtering the extractive solution under reduced pressure, concentrating at high temperature, purifying, vacuum drying, pulverizing, sterilizing, and sieving with 100 mesh sieve to obtain herba cistanches extract.
Comparative example 3
The only difference compared with example 3 is that the preparation method of the honeysuckle extract is different, and the rest operation is unchanged.
Body milk, the composition of which is the same as in example 3; the procedure for the preparation of body milk was as in example 3.
The preparation method of the honeysuckle extract comprises the following steps:
weighing flos Lonicerae, adding into 8 times of 45% ethanol water solution, soaking under normal pressure, decocting for 2 hr, and filtering; decolorizing the obtained filtrate, and concentrating to obtain flos Lonicerae extract.
Comparative example 4
Compared with example 3, the only difference is that the honeysuckle extract is replaced by dandelion extract, and the rest operation is unchanged.
The body lotion comprises the following components in parts by weight:
30 parts of glycerin, 25 parts of betaine, 15 parts of lauramidopropyl betaine, 0.003 part of folic acid, 2 parts of bamboo leaf flavone, 5 parts of litchi extract, 2 parts of cistanche extract and 2 parts of dandelion extract.
The preparation method of the body milk comprises the following steps:
(1) Placing glycerol, betaine, lauramidopropyl betaine, folic acid, bamboo leaf flavone, litchi extract, cistanche extract and dandelion extract in a reactor, and stirring and dispersing for 55min at 5000r/min under ultrasonic wave of 20KHz to obtain a dispersion;
(2) Step (2) was performed as in example 3.
In this example, the sources of bamboo leaf flavone, litchi extract and cistanche extract are the same as in example 3.
The preparation method of the dandelion extract comprises the following steps: weighing herba Taraxaci, adding into 4 times of 75% ethanol water solution, soaking under 20kpa condition, heating, decocting, and filtering; decolorizing the obtained filtrate, and concentrating to obtain herba Taraxaci extract.
Effect example
1. Elasticity test
Female volunteers 35 persons within 50-70kg of age 25-35 years old were selected and randomized into 7 groups of 5 persons each of example 1-3 and comparative example 1-4, respectively. Wherein the groups of examples 3-5 and comparative examples 1-4 were in accordance with 0.05g/cm 2 The dosage of (2) is 1 time in the morning and at night on the lower abdomen; during use, the diet is balanced. The skin elasticity of the lower abdomen before and after taking the anti-wrinkle product was measured by using a skin elasticity tester MPA580 of the company CK, germany, for 6 weeks, and the R2 value (R2 value means the ratio of the rebound quantity of the skin without negative pressure to the maximum stretching quantity with negative pressure, the ratio is approximately 1, which indicates that the better the skin elasticity). The results are shown in Table 1.
TABLE 1 skin elasticity
Test group Initial skin elasticity value R2 Skin elasticity value after 6 weeks
Example 3 0.61±0.07 0.88±0.19
Example 4 0.63±0.16 0.85±0.23
Example 5 0.59±0.09 0.86±0.17
Comparative example 1 0.58±0.22 0.76±0.21*
Comparative example 2 0.67±0.13 0.80±0.34*
Comparative example 3 0.62±0.15 0.72±0.08*
Comparative example 4 0.55±0.09 0.68±0.25*
Note that: p < 0.05 compared to control group.
Therefore, the body milk provided by the invention has better effect of tightening skin and increasing elasticity, and meanwhile, the types of the body milk components and the preparation method thereof have a certain influence on the effect.
2. Evaluation of whitening Effect
The body milks prepared in examples 3 to 5 and comparative examples 1 to 4 were tested for their whitening effect with reference to "T/SHRH 015-2018 cosmetic-tyrosinase activity inhibition test method".
1.5g/L of L-dopa was prepared using 0.01mol/L of disodium hydrogen phosphate-citric acid buffer at pH6.8 as a substrate, 40. Mu.L of the substrate was added to the well plate, and 80. Mu.L of disodium hydrogen phosphate-citric acid buffer and 40. Mu.L of the test substance (body milk samples prepared in examples 3 to 5 and comparative examples 1 to 4) were added. Then, 40. Mu.L of 100U/ml tyrosinase was added, and after mixing, the mixture was reacted in a water bath at 30℃for 30 minutes, and absorbance was measured at 490nm using a DR6000 ultraviolet-visible light spectrophotometer. And calculating the inhibition rate of tyrosinase activity.
Inhibition ratio = [1- (T-T) 0 )/(C-C 0 )]×100%;
Wherein C is absorbance (absorbance value of enzyme reacting with substrate) when no sample is added with enzyme, C 0 For the absorbance without enzyme addition without sample (solvent background absorbance value), T is the absorbance with enzyme addition with sample (sample tube absorbance value), T 0 Absorbance without enzyme addition to the sample (sample background absorbance).
TABLE 2 inhibition of tyrosinase activity
Test group Tyrosinase activity inhibition rate%
Example 3 85.21
Example 4 84.36
Example 5 83.64
Comparative example 1 66.28
Comparative example 2 79.45
Comparative example 3 77.67
Comparative example 4 73.59
Therefore, the body milk provided by the invention has a good whitening effect, and meanwhile, the types of the body milk components and the preparation method thereof have a certain influence on the whitening effect.
The foregoing detailed description is directed to one of the possible embodiments of the present invention, which is not intended to limit the scope of the invention, but is to be accorded the full scope of all such equivalents and modifications so as not to depart from the scope of the invention.

Claims (6)

1. The body milk comprises the following components in parts by weight: 15-40 parts of glycerin, 15-30 parts of betaine, 10-20 parts of lauramidopropyl betaine, 0.0001-0.005 part of folic acid compounds, 1-5 parts of bamboo leaf flavone, 2-8 parts of litchi extract, 1-3 parts of cistanche extract and 1-3 parts of honeysuckle extract;
the preparation method of the cistanche extract comprises the following steps:
a: pulverizing herba cistanches, reflux-extracting with 70-80% ethanol water solution, concentrating the extractive solution, and drying to obtain extract;
b: dissolving the extract in water, extracting with ethyl acetate or n-butanol for 2-3 times, filtering under reduced pressure, mixing filtrates, concentrating at high temperature, purifying, vacuum drying, pulverizing, sterilizing, and sieving with 100-120 mesh sieve to obtain herba cistanches extract; detecting herba cistanches extract sample by HPLC, wherein the content of echinacoside in herba cistanches extract reaches 60-80% to be qualified;
the times of reflux extraction in the step A are 2-3 times, and each time of reflux extraction is 1.5-2 hours; the addition amount of the ethanol aqueous solution is 2-8 times of the mass of cistanche; the relative density of the extract is 1.05-1.15;
the preparation method of the honeysuckle extract comprises the following steps:
weighing flos Lonicerae, adding into ethanol water solution, soaking under negative pressure, decocting under heating, and filtering; decolorizing the obtained filtrate, and concentrating to obtain flos Lonicerae extract;
wherein the mass concentration of the ethanol aqueous solution is 45-75%, and the dosage is 4-8 times of the mass of the honeysuckle; the negative pressure condition is that the pressure is 20-60kpa;
the folic acid compounds are one or more of folic acid, leucovorin, L-methyl folic acid, folic acid medicinal salt, folic acid or folic acid medicinal salt active metabolite and substances capable of being metabolized and/or generating folic acid in vivo.
2. Body milk according to claim 1, characterized in that it comprises the following components in parts by weight: 30 parts of glycerin, 25 parts of betaine, 15 parts of lauramidopropyl betaine, 0.003 part of folic acid compounds, 2 parts of bamboo leaf flavonoids, 5 parts of litchi extract, 2 parts of cistanche extract and 2 parts of honeysuckle extract.
3. The body milk of claim 1, wherein the bamboo flavone is prepared by the following steps:
taking bamboo leaves, steaming with steam, adding cellulase and pectase, performing enzymolysis at 30-45deg.C, filtering, adsorbing filtrate with Amberlite XAD-7 macroporous resin, eluting with 45-75% ethanol water solution, collecting eluate, and drying to obtain bamboo leaf flavone.
4. The body milk of claim 1, wherein the litchi extract is prepared by the steps of:
pulverizing fructus litchi pulp, filtering, sterilizing fructus litchi juice, inoculating yeast and lactobacillus, fermenting, separating and purifying the fermentation product, and filtering to obtain fructus litchi extract with molecular weight less than 5000 Da.
5. A method of preparing body milk according to claim 1, comprising the steps of:
(1) Placing glycerol, betaine, lauramidopropyl betaine, folic acid compounds, bamboo leaf flavone, litchi extract, cistanche extract and honeysuckle extract in a reactor, and performing ultrasonic treatment and stirring dispersion to obtain a dispersion;
(2) And (3) transferring the dispersion into a preheated homogenizing emulsifying pot, heating, homogenizing and emulsifying, and naturally cooling to normal temperature.
6. The method for preparing body milk according to claim 5, wherein in the step (1), the ultrasonic frequency is 20-40KHz, the stirring dispersion speed is 5000-6000r/min, and the dispersion time is 35-55min; in the step (2), the preheating temperature is 60-70 ℃; the temperature during emulsification is 75-80 ℃; the homogenization and emulsification are carried out for 2-4 minutes.
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