CN113101985B - Detection chip and detection system - Google Patents

Abstract

A detection chip and a detection system include a sample injection structure, a sample detection structure and a sample filter structure. The sample injection structure is used to inject the sample to be tested, the sample detection structure is used to make the tested sample can be detected, and the sample filter structure is between the sample injection structure and the sample detection structure and is respectively connected with the sample injection structure communicated with the sample detection structure, so as to filter the injected sample to be tested by lateral chromatography and transmit the filtered sample to be tested to the sample detection structure. The detection chip can make the detected sample purer through the sample filtering structure, so that the detection result is more accurate, and it is beneficial to realize the thinning of the detection chip.

Description

Detection chips and detection systems

technical field

Embodiments of the present disclosure relate to a detection chip and a detection system.

Background technique

Microfluidic chip technology integrates basic operation units such as sample preparation, reaction, separation, detection, etc. involved in the fields of biology, chemistry, and medicine into a chip with micron-scale microchannels, and automatically completes the entire process of reaction and analysis. The chip used in this process is called a microfluidic chip, also known as a Lab-on-a-chip. Microfluidic chip technology has the advantages of less sample consumption, fast analysis speed, easy to make into a portable instrument, suitable for instant and on-site analysis, etc., and has been widely used in many fields such as biology, chemistry and medicine.

SUMMARY OF THE INVENTION

At least one embodiment of the present disclosure provides a detection chip, which includes a sample injection structure, a sample detection structure, and a sample filter structure. The sample injection structure is used for injecting the sample to be detected, the sample detection structure is used to make the detected sample can be detected, and the sample filtering structure is between the sample injection structure and the sample detection structure and is respectively connected with the sample injection structure communicated with the sample detection structure, so as to filter the injected sample to be tested by lateral chromatography and transmit the filtered sample to be tested to the sample detection structure.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the sample filtering structure includes a filtering layer, and the filtering layer is configured to receive the detected sample from the sample injection structure on the first side, and the sample filtering structure is configured to receive the detected sample from the sample injection structure on the first side. The tested sample is filtered in the plane where the filter layer is located, and the filtered tested sample is output on a second side opposite to the first side.

For example, in the detection chip provided by at least one embodiment of the present disclosure, the sample injection structure and the sample filter structure are connected through a first channel, and the sample filter structure and the sample detection structure are connected through a second channel.

For example, in the detection chip provided by at least one embodiment of the present disclosure, on the first side of the filter layer, the first channel and the filter layer are at least partially in a direction perpendicular to the plane where the filter layer is located. overlapping to inject the tested sample from the sample injection structure into the filter layer for filtration.

For example, in the detection chip provided by at least one embodiment of the present disclosure, on the second side of the filter layer, the second channel and the filter layer are at least partially in a direction perpendicular to the plane where the filter layer is located. overlapping to receive the tested sample after filtering.

For example, in the detection chip provided by at least one embodiment of the present disclosure, in a direction perpendicular to the plane where the filter layer is located, the first channel and the second channel are respectively connected to different layer surfaces or surfaces of the filter layer. same layer surface.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the sample filtering structure further includes a filter cavity for accommodating the filter layer, the filter cavity includes a first opening and a second opening, and the first opening is used for The detected sample is input, and the second opening is used for outputting the filtered detected sample, wherein the second opening is connected to the first end of the second channel, and the sample detection structure is connected to the the second end of the second channel.

For example, the detection chip provided by at least one embodiment of the present disclosure further includes a first substrate and an adhesive layer, wherein the filter cavity is provided on the first substrate, and the adhesive layer is attached to the first substrate on the surface of the substrate and fix the filter layer in the filter cavity.

For example, in the detection chip provided by at least one embodiment of the present disclosure, the first channel and the second channel are provided in the first substrate.

For example, in the detection chip provided by at least one embodiment of the present disclosure, the sample injection structure and the sample detection structure are disposed on the first substrate.

For example, the detection chip provided by at least one embodiment of the present disclosure further includes a second substrate, wherein the second substrate is laminated with the first substrate and combined by the adhesive layer, the adhesive layer defines the the first opening and the second opening of the filter cavity, the first channel and the second channel are formed in the second substrate and communicate with the first opening and the second opening, respectively .

For example, in the detection chip provided by at least one embodiment of the present disclosure, the sample injection structure and the sample detection structure are disposed on the second substrate.

For example, in the detection chip provided by at least one embodiment of the present disclosure, the sample detection structure includes a plurality of detection units and a plurality of first detection flow channels, and at least one of the detection units is communicated with all the first detection flow channels through a corresponding first detection flow channel. The sample filter structure is described.

For example, in the detection chip provided by at least one embodiment of the present disclosure, the width of the first detection flow channel is 0.1 mm-1 mm, and the depth is 0.1 mm-1 mm.

For example, in the detection chip provided by at least one embodiment of the present disclosure, at least part of the first detection flow channel extends in a curved manner.

For example, in the detection chip provided by at least one embodiment of the present disclosure, at least a portion of the bent extension of the first detection flow channel is in the shape of a broken line or an S shape.

For example, in the detection chip provided by at least one embodiment of the present disclosure, at least a portion of the bending extension of the first detection flow channel includes 2-20 bends, and between two adjacent bends The length of the runner is 2mm-20mm.

For example, in the detection chip provided by at least one embodiment of the present disclosure, when at least a part of the bending extension of the first detection flow channel is in the shape of a folded line, the bending angle of the bending is 5°-120° .

For example, in the detection chip provided in at least one embodiment of the present disclosure, the detection unit includes a detection chamber for accommodating the sample to be detected, and the detection chamber has an exhaust hole and a gas-permeable liquid-resistance film covering the exhaust hole.

For example, in the detection chip provided by at least one embodiment of the present disclosure, the sample injection structure includes a sampling structure mounting portion for receiving the sampling structure.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the sample injection structure further includes a reagent pool, and after the sampling structure is installed in the sampling structure mounting portion, the sampling structure can communicate with the reagent pool , and the reagent pool is also configured to communicate with the sample filter structure.

For example, in the detection chip provided by at least one embodiment of the present disclosure, the sample injection structure further includes a first sealing layer located on the first side of the reagent cell, and the first sealing layer is used for the first sealing layer on the first side The reagent pool is sealed, and after the sampling structure is installed on the sampling structure mounting portion, the sampling structure can pierce the first sealing layer to communicate with the reagent pool.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the sample injection structure further includes a second sealing layer located on the second side of the reagent cell, and the second sealing layer is used for the second side. The reagent pool is sealed, and the first side of the reagent pool and the second side of the reagent pool face each other, when the second sealing layer is punctured, the reagent pool and the sample filter structure Connected.

For example, in the detection chip provided by at least one embodiment of the present disclosure, the sample injection structure further includes an elastic membrane and an action channel located on the second side of the reagent cell, and the second sealing layer is sandwiched between the elastic membrane and the reagent cell, the acting channel allows when an external force acts on the elastic membrane to deform the elastic membrane, the external force can also act on the second sealing layer to pierce the second seal Floor.

For example, in the detection chip provided by at least one embodiment of the present disclosure, the material of the elastic film is a composite polymer material.

For example, the detection chip provided in at least one embodiment of the present disclosure further includes a first substrate and an adhesive layer, wherein the sampling structure mounting portion and the reagent cell are disposed on the first substrate, and the adhesive layer A layer is attached to the surface of the first substrate and secures the elastic membrane to the first substrate.

For example, in the detection chip provided by at least one embodiment of the present disclosure, the adhesive layer at least partially exposes the second sealing layer, so that the deformed elastic film can act on the second sealing layer to puncture the second sealing layer.

For example, the detection chip provided by at least one embodiment of the present disclosure further includes a second substrate, wherein the second substrate is laminated with the first substrate and bonded by the adhesive layer, and the second substrate includes a substrate opening to provide the action channel.

For example, the detection chip provided by at least one embodiment of the present disclosure further includes a sample mixing chamber disposed between the sample injection structure and the sample filter structure.

At least one embodiment of the present disclosure provides a detection system. The detection system includes the detection chip, the sampling structure, and the packaging structure described above, wherein the detection chip includes a sampling structure mounting portion, and the sampling structure is mounted on the sampling structure. A sampling structure mounting part, the packaging structure is used for sealing the sampling structure.

For example, in the detection system provided by at least one embodiment of the present disclosure, the packaging structure is a silicone cap.

For example, in the detection system provided by at least one embodiment of the present disclosure, the packaging structure includes a sealing part and a fixing part, the sealing part is used for sealing the sampling structure, and the fixing part is used for fixing the sampling structure on the on the detection chip.

For example, the detection system provided by at least one embodiment of the present disclosure further includes a movable first push rod, and the first push rod is disposed on a side of the packaging structure away from the sampling structure.

For example, in the detection system provided by at least one embodiment of the present disclosure, the detection chip further includes a reagent pool and a second sealing layer, the detection system further includes a movable second ejector rod, and the second ejector rod is used for The second sealing layer is punctured.

Description of drawings

In order to explain the technical solutions of the embodiments of the present disclosure more clearly, the accompanying drawings of the embodiments will be briefly introduced below. Obviously, the drawings in the following description only relate to some embodiments of the present disclosure, rather than limit the present disclosure. .

1 is a perspective view of a three-dimensional structure of a detection system provided by at least one embodiment of the present disclosure;

2 is a perspective structural perspective view of an upper substrate of a detection chip provided by at least one embodiment of the present disclosure;

3 is a perspective structural view of a lower substrate of a detection chip provided by at least one embodiment of the present disclosure;

4 is an exploded top view of a detection system provided by at least one embodiment of the present disclosure;

5 is a bottom exploded view of a detection system provided by at least one embodiment of the present disclosure;

6 is a schematic plan view of a sample detection structure in a detection chip provided by at least one embodiment of the present disclosure;

7 is a perspective structural perspective view of a sampling structure in a detection system provided by at least one embodiment of the present disclosure;

8A is a perspective structural perspective view of a packaging structure in a detection system provided by at least one embodiment of the present disclosure;

8B is a bottom view of a packaging structure in a detection system provided by at least one embodiment of the present disclosure;

9 is a schematic diagram of a sample mixing operation performed by a detection system provided by at least one embodiment of the present disclosure;

10 is another schematic diagram of a sample mixing operation performed by a detection system provided by at least one embodiment of the present disclosure;

11 is a schematic diagram of a flow path of a sample to be detected in a detection system provided by at least one embodiment of the present disclosure;

FIG. 12 is a perspective structural perspective view of another detection system provided by at least one embodiment of the present disclosure;

13 is an exploded top view of a detection system provided by at least one embodiment of the present disclosure;

14 is a schematic diagram of a flow path of a sample to be detected in a detection system provided by at least one embodiment of the present disclosure;

FIG. 15 is an exploded top view of still another detection system provided by at least one embodiment of the present disclosure.

Detailed ways

In order to make the purpose, technical solutions and advantages of the embodiments of the present disclosure more clear, the technical solutions of the embodiments of the present disclosure will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present disclosure. Obviously, the described embodiments are some, but not all, embodiments of the present disclosure. Based on the described embodiments of the present disclosure, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the protection scope of the present disclosure.

Unless otherwise defined, technical or scientific terms used in this disclosure shall have the ordinary meaning as understood by one of ordinary skill in the art to which this disclosure belongs. As used in this disclosure, "first," "second," and similar terms do not denote any order, quantity, or importance, but are merely used to distinguish the various components. "Comprises" or "comprising" and similar words mean that the elements or things appearing before the word encompass the elements or things recited after the word and their equivalents, but do not exclude other elements or things. Words like "connected" or "connected" are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "Up", "Down", "Left", "Right", etc. are only used to represent the relative positional relationship, and when the absolute position of the described object changes, the relative positional relationship may also change accordingly.

In the design process of microfluidic chips, it is usually desirable to integrate as many functions of analysis and detection into the chip as possible to reduce the chip's dependence on external operations, thereby achieving automation and integration. For example, the sampling parts, mixing parts, filtering parts, and analysis and detection parts of the microfluidic chip can be integrated to realize the automation of the detection process. During the detection process of the microfluidic chip, the sampling part is first used to obtain the sample, and then the sample is fully mixed with the detection reagent (or the reagent that makes the sample more suitable for detection, such as diluent) in the mixing part, and then filtered. for the next detection operation. The filtering operation of the sample will improve the purity of the sample, which plays a crucial role in the detection process and detection results of the microfluidic chip.

At least one embodiment of the present disclosure provides a detection chip, which includes a sample injection structure, a sample detection structure, and a sample filter structure. The sample injection structure is used for injecting the sample to be detected, the sample detection structure is used to make the detected sample detectable, and the sample filter structure is between the sample injection structure and the sample detection structure and communicated with the sample injection structure and the sample detection structure respectively, so as to connect the sample to the sample injection structure and the sample detection structure. The injected test sample is filtered by lateral chromatography and the filtered test sample is transferred to the sample detection structure.

The detection chip of at least one embodiment of the present disclosure integrates the sample injection function, the sample detection function and the sample filtering function, thereby enriching the functions of the detection chip, improving the integration degree of the detection chip, and helping to realize the miniaturization of the detection chip. In addition, the sample filtering structure of the detection chip filters the injected sample to be tested in a lateral chromatographic manner, thereby further contributing to the reduction of the overall shape of the detection chip. The miniaturized and thin detection chip also contributes to the realization of a portable detection and analysis device.

At least one embodiment of the present disclosure provides a detection system, which includes the above-mentioned detection chip, sampling structure, and packaging structure. The detection chip includes a sampling structure mounting portion, the sampling structure is mounted on the sampling structure mounting portion, and the packaging structure is used for sealing the sampling structure.

The detection chip and detection system of the present disclosure will be described below through several specific embodiments.

At least one embodiment of the present disclosure provides a detection chip and a detection system, and the detection system includes the detection chip and components used together with the detection chip. For example, FIG. 1 shows a perspective view of a three-dimensional structure of a detection system provided by this embodiment, FIG. 2 shows a perspective view of a three-dimensional structure of an upper substrate of a detection chip provided by this embodiment, and FIG. Figure 4 shows a top exploded view of the detection system, and Figure 5 shows a bottom exploded view of the detection system provided by this embodiment.

As shown in FIGS. 1-5 , the detection chip 100 includes a sample injection structure 101 , a sample detection structure 102 and a sample filter structure 103 . The sample injection structure 101 is used for injecting the sample to be detected. The sample detection structure 102 is used to allow the detected sample to be detected. For example, the sample detection structure 102 has a detection reagent, and the detected sample is mixed with the detection reagent before performing the detection operation. For example, in some examples, the detection reagent is a lyophilized reagent, and the sample to be tested can be thawed with the lyophilized reagent and undergo the required reaction, so as to be suitable for subsequent detection operations, and these detection operations can be optical detection, etc. as required , which is not limited by the embodiments of the present disclosure. For example, the sample filtering structure 103 is between the sample injection structure 101 and the sample detection structure 102 and communicates with the sample injection structure 101 and the sample detection structure 102, respectively, so as to filter the injected sample to be tested in a lateral chromatographic manner and to filter The latter sample to be tested is transferred to the sample detection structure 102 . The filtration method of lateral chromatography can make the sample to be tested be fully and uniformly filtered, which can make the filtered sample to be tested more pure, improve the detection effect, and reduce the space used by the filtration method of lateral chromatography. In addition, it is in the form of a thin layer, which contributes to the thinning of the detection chip 100 .

For example, in some embodiments, the sample filter structure 103 includes a filter layer 1031, as shown in FIG. 3, the filter layer 1031 is configured to receive the detected sample from the sample injection structure 101 on the first side 1031A, along the filter layer 1031 The detected sample is filtered in-plane and the filtered detected sample is output on a second side 1031B opposite the first side 1031A. Thus, the sample to be tested enters the filter layer 1031 from the first side of the filter layer 1031, passes through the filter layer 1031 laterally, and is output from the second side of the filter layer 1031, because the filter path of the sample to be tested is the horizontal dimension of the filter layer 1031 ( length or width), which is greater than or much greater than the thickness of the filter layer 1031, so that the tested sample can be sufficiently filtered. For example, when the amount of the tested sample is large, since the filtering path of the tested sample is basically the same, the tested sample can also be fully and uniformly filtered.

For example, in some embodiments, as shown in FIG. 1 , the sample injection structure 101 and the sample filter structure 103 are connected by a first channel 104, and the sample filter structure 103 and the sample detection structure 102 are connected by a second channel 105; In some embodiments, the sample injection structure 101 and the sample filter structure 103 may be directly connected, and the sample filter structure 103 and the sample detection structure 102 may be directly connected. For example, in some embodiments, the end of the first channel 104 connected to the sample filter structure 103 includes a first channel cavity 1041, so that the sample to be tested can be uniformly input into the sample filter structure 103, and to prevent the sample to be tested in the first The junctions of the channel 104 and the filter structure 103 are concentrated. For example, the first channel cavity 1041 is, for example, in the shape of a circle, an ellipse, or a water drop shape (as shown in the figure).

For example, in some embodiments, on the first side 1031A of the filter layer, the first channel 104 at least partially overlaps the filter layer 1031 in a direction perpendicular to the plane of the filter layer 1031 for injecting the sample from the sample injection structure 101. The sample to be detected is injected into the filter layer 1031 for filtration, so that the contact area between the first channel 104 and the filter layer 1031 is larger, so that it is more convenient to inject the detected sample into the filter layer 1031 .

For example, as shown in FIG. 1 to FIG. 3 , after the upper substrate of the detection chip is combined with the lower substrate, at least part of the first channel 104 , such as the first channel cavity 1041 , is perpendicular to the plane of the filter layer 1031 with the filter layer 1031 . overlap in the direction. For example, the first channel cavity 1041 extends above the filter layer 1031 so as to overlap with the filter layer 1031 . Therefore, the first channel 104 can inject the sample to be tested into the filter layer 1031 in a direction perpendicular to the plane of the filter layer 1031, so that the sample to be tested can be uniformly dispersed before entering the filter layer 1031, so as to avoid filtration caused by sample concentration not effectively. In addition, the overlapping area of the first channel cavity 1041 and the filter layer 1031 is relatively large, which is beneficial to the dispersion of the detected samples and avoids the detected samples from being concentrated at the connection position of the filter layer 1031 and the first channel 104 . As a result, the above-mentioned filtering structure can make the sample to be tested be fully and uniformly filtered, thereby making the filtered sample to be tested more pure.

For example, in some embodiments, on the second side 1031B of the filter layer, the second channel 105 and the filter layer 1031 at least partially overlap in a direction perpendicular to the plane of the filter layer 1031 to receive the filtered test sample . For example, in some embodiments, at least a portion of the second channel 105 extends above or below the filter layer 1031 so as to overlap with the filter layer 1031 . Thus, the second channel 105 can output the detected sample from the filter layer 1031 in a direction perpendicular to the plane where the filter layer 1031 is located.

For example, in some embodiments, in the direction perpendicular to the plane of the filter layer 1031, the first channel 104 and the second channel 105 are respectively connected to different layer surfaces of the filter layer 1031 (that is, the length direction and the width direction of the filter layer are defined by lateral surface) or the surface of the same layer. It should be noted that the layer surface mentioned in the embodiments of the present disclosure refers to the surface of the filter layer 1031 parallel to the plane where the filter layer 1031 is located, that is, the upper surface and the lower surface of the filter layer 1031 shown in the figure. For example, the first channel 104 and the second channel 105 are respectively connected to the upper surface and the lower surface of the filter layer 1031 , and may also be connected to the upper surface or the lower surface of the filter layer 1031 at the same time. For example, in some embodiments, the first channel 104 may also be connected to the layer surface of the filter layer 1031 , and the second channel 105 may be connected to the side surface of the filter layer 1031 , and the side surface is between the upper surface and the lower surface of the filter layer 1031 . s surface. The embodiment of the present disclosure does not limit the specific connection manner of the first channel 104 and the second channel 105 , as long as the first channel 104 can inject the sample to be detected into the filter layer 1031 along a direction perpendicular to the plane of the filter layer 1031 .

For example, in some embodiments, as shown in FIG. 3 , the sample filter structure 103 further includes a filter cavity 1032 that accommodates the filter layer 1031 . As shown in FIG. 1 , the filter cavity 1032 includes a first opening 1032A and a second opening 1032B. The first opening 1032A is used for inputting the sample to be tested, and the second opening 1032B is used for outputting the filtered sample to be tested. The second opening 1032B is connected to the first end (eg, the right end shown in the figure) of the second channel 105 , and the sample detection structure 102 is connected to the second end (eg, the left end shown in the figure) of the second channel 105 .

For example, in some embodiments, the detection chip further includes a first substrate and an adhesive layer, the filter cavity 1032 is disposed on the first substrate, and the adhesive layer is attached to the surface of the first substrate and fixes the filter layer 1031 on the first substrate. In the filter cavity 1032, and the first substrate can also be used to form/support other functional structures. For example, the detection chip further includes a second substrate, the second substrate is laminated with the first substrate and bonded by an adhesive layer, and the second substrate can also form/support other functional structures, or, the first substrate and the second substrate jointly Form/support other functional structures.

For example, in the embodiment shown in FIG. 1 to FIG. 5 , the detection chip includes an upper substrate 100A and a lower substrate 100B, the lower substrate 100B is implemented as an example of the above-mentioned first substrate, and the upper substrate 100A is implemented as an example of the above-mentioned second substrate, The upper substrate 100A and the lower substrate 100B are bonded by an adhesive layer 100C. For example, the material of the upper substrate 100A may be polystyrene (PS) or polymethyl methacrylate (PMMA), and the material of the lower substrate 100B may also be polystyrene (PS) or polymethyl methacrylate (PMMA). )Wait. The adhesive layer 100C may include an adhesive material such as an acrylic adhesive, for example, may be implemented as a double-sided tape. For example, the upper substrate 100A, the adhesive layer 100C and the lower substrate 100B have substantially the same shape, so that the adhesive layer 100C can better realize the bonding between the upper substrate 100A and the lower substrate 100B.

For example, the adhesive layer 100C defines a first opening 1032A and a second opening 1032B of the filter cavity 1032 , for example, the adhesive layer 100C has a first opening and a second opening, and the adhesive layer 100C is attached to the surface of the lower substrate 100B to filter The layer 1031 is fixed in the filter cavity 1032 such that the first opening and the second opening of the adhesive layer 100C are formed as the first opening 1032A and the second opening 1032B of the filter cavity 1032 . Therefore, the adhesive layer 100C not only plays the role of bonding, but also can play the role of sealing the filter cavity 1032 , and provides a flow channel for the sample to be detected through the first opening and the second opening, so as to facilitate the realization of the sample filter structure 103 Lateral filter function.

For example, the first channel 104 and the second channel 105 may both be formed in the upper substrate 100A and communicate with the first opening 1032A and the second opening 1032B, respectively. For example, the sample injection structure 101 and the sample detection structure 102 are also disposed on the upper substrate 100A. Therefore, in the detection chip shown in FIGS. 1-5 , for example, referring to FIG. 11 , the sample injection structure 101 located on the upper substrate 100A can pass the detected sample through the first opening 1032A in a direction perpendicular to the plane where the filter membrane 1031 is located. Input to the sample filtering structure 103 on the lower substrate 100B, and output the filtered sample to the sample detecting structure 102 on the upper substrate 100A through the second opening 1032B in a direction perpendicular to the plane of the filter membrane 1031 . Thereby, the functions of lateral chromatography and vertical liquid feeding of the sample filter structure 103 are realized.

For example, the sample detection structure 102 includes a plurality of detection units 1021 and a plurality of first detection flow channels 1022 , and at least one detection unit 1021 communicates with the sample filter structure 103 through a corresponding first detection flow channel 1022 . For example, the detection unit 1021 is communicated with the second end of the second channel 105 through the corresponding first detection flow channel 1022 (eg, the left end of the second channel 105 shown in the figure), so that the sample filtered by the sample filter structure 103 is filtered. The detection sample can flow into the detection unit 1021 from the second channel 105 and the first detection flow channel 1022 .

For example, each detection unit 1021 is communicated with the second end of the second channel 105 through the corresponding first detection flow channel 1022 . For example, the second end of the second channel 105 has an auxiliary connection structure 1051 , and the extension direction of the auxiliary connection structure 1051 is perpendicular to the extension direction of the second channel 105 . For example, the first detection flow channels 1022 are all connected to the second end of the second channel 105 through the auxiliary connection structure 1051 , thereby facilitating the arrangement of the first detection flow channels 1022 and making the length of each first detection flow channel 1022 and shape etc. are basically the same.

For example, in some embodiments, as shown in FIG. 6 , the width W of the first detection flow channel 1022 may be 0.1 mm-1 mm, such as 0.3 mm, 0.5 mm or 0.7 mm, etc. The depth of the first detection flow channel 1022 ( That is, the dimension in the direction perpendicular to the plane where the upper substrate 100A is located) may be 0.1 mm-1 mm, for example, 0.3 mm, 0.5 mm, or 0.7 mm. Therefore, the detected sample can have a suitable flow rate and flow rate in the first detection flow channel 1022 .

For example, at least a portion of the first detection flow channel 1022 extends curvedly. For example, at least a part of the bent extension of the first detection flow channel 1022 is in a broken line shape or an S shape. For example, at least a portion of the bent extension of the first detection flow channel 1022 may include 2-20 bends 1022A, such as 5, 8, 12, 16, etc., between two adjacent bends 1022A. The length L of the flow channel can be 2mm-20mm, such as 3mm, 5mm, 10mm or 15mm, etc.

For example, as shown in FIG. 6 , when at least part of the bending extension of the first detection flow channel 1022 is in the shape of a broken line, the bending angle α of the bending 1022A may be 5°-120°, such as 30°, 60° or 90° etc.

For the above-mentioned design of the first detection flow channel 1022, since the first detection flow channel 1022 has a bent and extended part, on the one hand, the bent and extended part can extend the flow channel of the sample to be detected; The bent 1022A of the extended part can also increase the flow resistance of the sample to be detected, so that the sample to be detected is not easy to flow back, thereby preventing the sample to be detected in the plurality of detection units 1021 from crosstalk, thereby preventing the sample to be detected. Therefore, the design of the first detection flow channel 1022 can improve the detection accuracy and detection quality.

For example, in some embodiments, the detection unit 1021 includes a detection chamber (eg, a columnar structure shown in the figure) for accommodating the sample to be detected, and the detection chamber has an exhaust hole 1021A and a gas-permeable liquid-blocking film 1021B covering the exhaust hole 1021A. When the sample to be detected flows into the detection chamber, the pressure in the detection chamber increases, the exhaust hole 1021A can discharge the excess air in the detection chamber to balance the air pressure, and the gas-permeable liquid-barrier film 1021B has the function of gas permeability but liquid impermeability. This prevents the sample being tested from flowing out of the detection chamber. When the detection unit 1021 is formed in the upper substrate 100A, the vent hole 1021A may be formed on the side surface of the upper substrate 100A (the left end surface of the upper substrate 100A as shown in FIG. 2 or FIG. 4 ), and the gas-permeable liquid-barrier film 1021B may be It is pasted on the side surface of the upper substrate 100A, thereby covering the exhaust hole 1021A.

For example, in some embodiments, the gas-permeable and liquid-resistance films 1021B covering the vent holes 1021A of the plurality of detection units 1021 are of an integrated structure. At this time, the gas-permeable liquid-resistance film 1021B of the integrated structure can cover the side with the air vents 1021A of the plurality of detection units 1021 in the form of a whole surface (as shown in FIG. 4 and FIG. 5 ), so that the detection chip can be simplified. Structure and production difficulty. For example, the gas-permeable and liquid-blocking film 1021B may be an ePTFE (expanded polytetrafluoroethylene) gas-permeable and liquid-blocking film, which is not limited in the embodiment of the present disclosure.

For example, in some embodiments, the sample injection structure 101 includes a sampling structure mounting portion 1011 for receiving a sampling structure, eg, mounting a sampling structure having a sample to be tested therein. For example, the sample injection structure 101 further includes a reagent pool 1012, and the reagent pool 1012 is used to store detection reagents or diluents and other reagents that make the samples more suitable for detection (the reagent pool 1012 will be used as an example for storing the diluents), for example, as As shown in FIG. 4, the reagent well 1012 is formed in the upper substrate 100A. After the sampling structure is installed on the sampling structure mounting part 1011 , the sampling structure can communicate with the reagent pool 1012 , for example, the sampling structure is further pressed to communicate with the reagent pool 1012 , and the reagent pool 1012 can also communicate with the sample filter structure 103 .

For example, in some embodiments, the mixing ratio of the sample to be tested and the diluent is determined. For example, the volume of the diluent in the reagent cell 1012 can be selected and adjusted to facilitate obtaining the desired mixing ratio. For example, the total amount of samples obtained by the sampling structure is known (eg, the total amount of samples obtained by the sampling structure is a fixed value or can be read), so the volume of the diluent in the reagent cell 1012 can be selected to control the Detect the mixing ratio of the sample and the diluent, realize the quantification of the sample, and obtain the sample with a certain concentration, etc.

For example, the sample injection structure 101 may further include a first sealing layer 1013 on the first side (shown as the upper side in the figure) of the reagent cell 1012, and the first sealing layer 1013 is used to seal the reagent cell 1012 on the first side. After the sampling structure is installed on the sampling structure mounting portion 1011 , the sampling structure can be pressed to pierce the first sealing layer 1013 to communicate with the reagent pool 1012 .

For example, the sample injection structure 101 may further include a second sealing layer 1014 on the second side (shown as the lower side in the figure) of the reagent cell 1012, the second sealing layer 1014 is used to seal the reagent cell 1012 on the second side, the reagent The first side of the cell 1012 and the second side of the reagent cell 1012 face each other. After the second sealing layer 1014 is punctured, the reagent cell 1012 can communicate with the sample filter structure 103 .

For example, the first sealing layer 1013 and/or the second sealing layer 1014 may be aluminum foil. In this case, the first sealing layer 1013 and/or the second sealing layer 1014 may be formed on both sides of the reagent cell 1012 by heat sealing, respectively. , thereby forming a closed reagent storage space. For example, in other examples, the first sealing layer 1013 and/or the second sealing layer 1014 may also be other materials with sealing functions such as polymethyl methacrylate (PMMA), polypropylene (PP), etc. A sealing layer 1013 and/or a second sealing layer 1014 can be respectively formed on both sides of the reagent pool 1012 by ultrasonic welding, thereby forming a sealed reagent storage space. The embodiment of the present disclosure does not limit the specific form of the first sealing layer 1013 and/or the second sealing layer 1014 .

For example, in some embodiments, the sample injection structure 101 may further include an elastic membrane 1015 and an action channel 1016 on the second side of the reagent cell 1012 , and the second sealing layer 1014 is sandwiched between the elastic membrane 1015 and the reagent cell 1012 . The adhesive layer 100C is sandwiched between the elastic film 1015 and the second sealing layer 1014, thereby bonding and fixing the elastic film 1015 and the second sealing layer 1014 relative to each other. Meanwhile, referring to FIG. 4 and FIG. 5 , corresponding to the action channel 1016 , the adhesive layer 100C further has a third opening 1001 , and the third opening 1001 is also located in the area where the elastic film 1015 and the second sealing layer 1014 are directly overlapped with each other, For example, the third opening 1001 is completely located in the region where the elastic film 1015 and the second sealing layer 1014 are facing and overlapping each other, and the region where the elastic film 1015 and the second sealing layer 1014 are facing and overlapping each other is completely bonded by the adhesive layer 100C fixed. The action channel 1016 allows when an external force acts on the elastic film 1015 to deform the elastic film 1015, the deformed elastic film 1015 partially passes through the third opening 1001 of the adhesive layer 100C, thereby in the case where the elastic film 1015 itself is not punctured The external force can also act on the second sealing layer 1014 to puncture the second sealing layer 1014. After the external force is removed, the elastic film 1015 will basically return to its original state.

For example, the material of the elastic film 1015 is a composite polymer material, such as a composite material of polystyrene (PS) and polyethylene terephthalate (PET). Therefore, the elastic film 1015 can have good elasticity and strength at the same time. The elastic film 1015 is bonded between the upper substrate and the lower substrate through, for example, the adhesive layer 100C. For example, in other examples, the elastic film 1015 can also be bonded between the upper substrate and the lower substrate by ultrasonic welding, photosensitive adhesive bonding, chemical solvent bonding, or laser welding, etc., which is not done in the embodiments of the present disclosure. limited.

For example, as described above, the adhesive layer 100C at least partially exposes the second sealing layer 1014 through the third opening 1001 to allow the deformed elastic membrane 1015 to act on the second sealing layer 1014 to puncture the second sealing layer 1014 . For example, the lower substrate 100B includes a substrate opening to provide the active channel 1016, eg, the elastic membrane 1015 completely covers the active channel 1016, the projection of the third opening 1001 of the adhesive layer 100C on the lower substrate 100B, eg, at least partially overlaps the active channel 1016, eg The active channel 1016 is completely covered.

For example, as shown in FIG. 1 to FIG. 5 , the detection system provided in this embodiment further includes a sampling structure 110 and a packaging structure 111 in addition to the above-mentioned detection chip. The sampling structure 110 is mounted on the sampling structure mounting portion 1011 of the sample injection structure 101 , and the packaging structure 111 is used for sealing the sampling structure 110 . For example, as shown in FIG. 4 and FIG. 5 , the sampling structure 110 can be mounted on the sampling structure mounting portion 1011 through a gasket 140 . The gasket 140 is, for example, a silicone material, so as to play a sealing and buffering role.

For example, in some embodiments, as shown in FIG. 7 , the sampling structure 110 may be a sampling needle with sample suction and mixing functions. For example, as shown in FIGS. 8A and 8B , the encapsulation structure 111 may be a silicone cap. For example, the sampling structure 110 and the packaging structure 111 can cooperate with each other to complete the sample mixing function.

For example, FIG. 9 shows a schematic cross-sectional view of the sample injection structure 101 when the sampling structure 110 is mounted on the sampling structure mounting portion 1011 , and the packaging structure 111 seals the sampling structure 110 . As shown in FIG. 9 , the sampling structure 110 includes a first suction channel 110A, a second suction channel 110B and a chamber 110C. A plurality of separation columns 110D are disposed in the second suction channel 110B. A first gap is formed between the separation column 110D and the channel wall of the second suction channel 110B, and a second gap is formed between adjacent separation columns 110D. The first suction channel 110A and the second suction channel 110B of the sampling structure are configured to absorb the detected sample (such as blood, body fluid, etc.) by capillary action, and the separation column 110D in the second suction channel 110B can prevent the flow through the second suction channel 110B. The sample to be detected in the suction channel 110B is branched. In the chamber 110C, after the sample to be detected is mixed with the diluent in the reagent cell, the sampling structure can play the role of sample mixing.

For example, as shown in FIGS. 8A and 8B , the package structure 111 includes a main body portion 111A and at least one vent hole 111B, such as a plurality of vent holes 111B, disposed at a peripheral position of the main body portion 111A. For example, these vent holes 111B are evenly distributed around the main body portion 111A. The exhaust hole 111B is configured to be in an open state or a closed state, respectively, when the main body portion 111A receives different forces. For example, in some embodiments, as shown in FIG. 8B , the vent hole 111B may be a triangular prism vent hole. When the force on the main body portion 111A is greater than or equal to a threshold value, the vent hole 111B is closed, and when the main body portion 111A receives a force greater than or equal to a threshold value, the vent hole 111B is closed. When the applied force is smaller than the threshold value, the vent hole 111B is in an open state. Thus, the internal pressure of the sampling structure 110 can be adjusted by applying different forces to the main body portion 111A of the packaging structure 111 .

For example, in some embodiments, the detection system further includes a movable first push rod 120 , and the first push rod 120 is disposed on a side of the packaging structure 111 away from the sampling structure 110 . Thus, the first ejector rod 120 can move up and down on the main body portion 111A of the packaging structure 111 to apply a force to the main body portion 111A. For example, the force exerted by the first ejector rod 120 on the main body portion 111A is adjustable, so that the pressure in the sampling structure 110 can be controlled. For example, the movement of the first push rod 120 may be driven by a driving device (eg, a stepping motor).

For example, as shown in FIG. 8A , the main body portion 111A may include a concave groove for guiding the force applying position of the first ejector rod 120 . For example, the shape of the concave groove is the same as that of the first ejector pin 120 , for example, the cross section thereof is circular. For example, the diameter of the concave groove is slightly larger than the diameter of the first ejector pin 120 and has steps, so as to facilitate guiding the force application position of the first ejector pin 120 .

For example, in some embodiments, the detection system may further include a movable second plunger 130 for piercing the second sealing layer 1014 . For example, the second push rod 130 applies force to the elastic membrane 1015 through the acting channel 1016 , so that the deformed elastic membrane 1015 can act on the second sealing layer 1014 to puncture the second sealing layer 1014 . For example, the movement of the second jack 130 may be driven by another driving device (eg, a stepping motor).

Hereinafter, the working process of the above detection system will be exemplarily introduced with reference to FIGS. 9-11 .

First, the sample to be detected is sucked by the sampling structure 110 . At this time, the sample to be detected can be sucked from the first suction channel 110A and the second suction channel 110B of the sampling structure 110 through capillary action. The sample to be detected may be, for example, blood, body fluid, etc., which is not limited in the embodiments of the present disclosure.

Then, the sampling structure 110 is mounted on the detection chip 100 , and the sampling structure 110 is fixed and sealed by using the packaging structure 111 . For example, after the sampling structure 110 is installed on the detection chip 100 , the bottom of the sampling structure 110 extends into the reagent pool 1012 of the detection chip 100 . For example, the upper surface of the reagent pool 1012 has a first sealing layer 1013 for sealing. When the sampling structure 110 is installed on the detection chip 100 , the sampling structure 110 can puncture the first sealing layer 1014 and connect with the reagent pool 1012 so that the sample to be tested can be mixed with the diluent in the reagent cell 1012. After the sampling structure 110 and the sealing structure 111 are installed, two connected sealing cavities are formed in the detection chip 100 , one is the first sealing cavity formed by the sampling structure 110 , and the other is the second sealing cavity formed by the reagent pool 1012 .

As shown in FIG. 9 , the first ejector rod 120 is used to apply a force to the main body 111A of the sealing structure 111 at a first speed. When the force is weak and less than the threshold pressure, the exhaust hole 111B of the sealing structure 111 is in an open state. , the sampling structure 110 can discharge air or overflow liquid through the exhaust hole 111B. When the applied force gradually increases to be greater than or equal to the threshold pressure, the vent hole 111B is in a closed state. At this time, the pressure inside the sampling structure 110 increases, so that the sample to be detected is pushed out of the sampling structure 110 and enters the reagent pool 1012, as indicated by the arrows in FIG. 9 . Thus, the sample to be detected can be mixed with the diluent in the reagent cell 1012 .

As shown in FIG. 10 , the first ejector rod 120 is withdrawn at the second speed. At this time, the main body 111A rebounds, and the mixture of the diluent and the tested sample in the reagent pool 1012 will be sucked back into the sampling structure 110 . Since in the sampling structure 110, the second suction channel 110B has a separation column 110D, and a plurality of narrow gaps are formed between the adjacent separation columns 110D and between the separation column 110D and the channel wall, so the diluent and the detected After the mixed liquid of the sample passes through the first suction channel 110A and the second suction channel 110B, its flow rate will be accelerated, and then the mixed liquid can be flushed into the chamber 110C of the sampling structure 110 at a relatively high speed, and will interact with the sample in the sampling structure 110. The test sample forms a swirling mixture, as shown by the arrow in Figure 10, thereby improving the mixing efficiency and making the diluent and the test sample mix more uniformly. At this point, one mixing operation is completed.

For example, the first speed at which the first mandrel 120 applies force to the main body 111A of the sealing structure 111 is greater than the second speed at which the first mandrel 120 is withdrawn, that is, the first mandrel 120 is quickly pressed down and slowly lifted up. , this operation helps to improve the mixing effect of the sample to be detected and the diluent in the sampling structure 110 .

For example, the above mixing operation can be performed multiple times to further improve the mixing effect of the tested sample and the diluent.

For example, after the sample to be tested and the diluent are sufficiently mixed, the second ejector pin 130 can be driven to move upward, so that the second ejector pin 130 passes through the action channel 1016 and the elastic membrane 1015 to the second sealing layer 1014 below the reagent cell 1012 Force is applied to puncture the second sealing layer 1014 . Since the elastic film 1015 has elasticity, it can return to its original state after the external force is removed. After the second sealing layer 1014 is punctured, the reagent cell 1012 communicates with the sample filter structure 103 .

For example, Figure 11 shows the flow path of the sample being tested in more detail. As shown in FIG. 11 , the first ejector rod 120 can be continuously pressed down slowly and continuously, so that the sample to be tested enters the sample filter structure 103 (the sample to be tested is input into the filter membrane 1031, for example, in a direction perpendicular to the plane where the filter membrane 1031 is located) , the filtered sample to be tested can be transported to the sample detection structure 102 during the continuous pressing of the first ejector rod 120 (the sample to be tested is output to the sample detection structure 102, for example, in a direction perpendicular to the plane where the filter membrane 1031 is located) , for example, into the detection chamber 1021 of the sample detection structure 102 . For example, the detection chamber 1021 has lyophilized reagents suitable for different detection items, so that after the detected sample can react with the lyophilized reagent, the sample detection structure 102 starts to perform detection and outputs the detection result. For example, during the detection process of the sample detection structure 102, the first ejector pin 120 is always kept in a depressed state to avoid backflow of the sample to be detected.

Therefore, the detection system can realize an automatic detection process, and the detection system can also obtain more accurate detection results.

For example, in some embodiments, the detection chip 100 may further include a sample mixing chamber (not shown in the figure) disposed between the sample injection structure 101 and the sample filter structure 103 . At this time, the tested sample and the diluent can also be mixed in the sample mixing chamber. For example, the mixing operation may be performed by the sampling structure 110 and the sample mixing chamber, or, in some embodiments, the sampling structure 110 may only have a sampling function, so that the mixing operation may be performed only in the sample mixing chamber, the present disclosure The embodiment does not limit this.

At least one embodiment of the present disclosure provides another detection chip and a detection system, and the detection system includes the detection chip. For example, FIG. 12 shows a perspective view of the three-dimensional structure of the detection system provided by this embodiment, and FIG. 13 shows an exploded top view of the detection system.

As shown in FIG. 12 and FIG. 13 , the detection chip 200 includes a sample injection structure 201 , a sample detection structure 202 and a sample filter structure 203 . The sample injection structure 201 is used for injecting the sample to be tested, the sample detection structure 202 is used to allow the tested sample to be detected, and the sample filter structure 203 is between the sample injection structure 201 and the sample detection structure 20 and is respectively connected with the sample injection structure 201 and the sample The detection structure 202 communicates to filter the injected sample to be tested by lateral chromatography and to transmit the filtered sample to be tested to the sample detection structure 202 .

For example, in some embodiments, the sample filter structure 203 includes a filter layer 2031 configured to receive the detected sample from the sample injection structure 201 on the first side 2031A, and to filter the detected sample in a plane along the filter layer 2031 , the filtered sample to be tested is output on the second side 2031B opposite to the first side 2031A, so that the filter layer 2031 can realize the lateral chromatographic filtering function.

For example, the sample injection structure 201 and the sample filter structure 203 are connected through the first channel 204 , and the sample filter structure 203 and the sample detection structure 202 are connected through the second channel 205 .

For example, on the first side 2031A of the filter layer 2031, the first channel 204 and the filter layer 2031 at least partially overlap in a direction perpendicular to the plane of the filter layer 2031, so as to inject the detected sample from the sample injection structure into the filter layer 2031 for filtering. For example, the first channel 204 overlaps with the filter layer 2031 at the side and the top of the filter layer 2031 , so that the sample to be detected can be input to the filter layer 2031 in the lateral direction and perpendicular to the plane of the filter layer 2031 at the same time. sample. Therefore, the detected sample can be uniformly dispersed before entering the filter layer 2031, so as to avoid the poor filtering effect caused by the concentration of the sample.

For example, one end of the first channel connected to the sample filtering structure 203 may further have a first channel cavity 2041 , for example, the first channel cavity 2041 overlaps with the filter layer 2031 at the top and side of the filter layer 2031 . The first channel cavity 2041 is, for example, in the shape of a circle, an ellipse, or a water drop shape (as shown in FIG. 12 ). Since the overlapping area of the first channel cavity 2041 and the filter layer 2031 is large, it is beneficial to the dispersion of the detected samples, and the detected samples are prevented from being concentrated at the connection position of the filter layer 2031 and the first channel 204 . Therefore, the above-mentioned filtering structure can make the sample to be tested be fully and uniformly filtered, thereby making the sample to be tested more pure.

For example, the sample filter structure 203 further includes a filter cavity 2032 for accommodating the filter layer 2031, and the filter cavity 2032 includes a first opening 2032A and a second opening 2032B. The first opening 2032A is used to input the sample to be tested, and the second opening 2032B is used to output the filtered sample to be tested. For example, the second opening 2032B is connected to the first end of the second channel 205 , and the sample detection structure 202 is connected to the second end of the second channel 205 .

For example, the detection chip further includes a first substrate and an adhesive layer, the filter cavity 2032 is disposed on the first substrate, and the adhesive layer is attached on the surface of the first substrate and fixes the filter layer 2031 in the filter cavity 2032 . For example, the detection chip further includes a second substrate, which is laminated with the first substrate and bonded by an adhesive layer.

For example, as shown in FIG. 13 , the detection chip includes an upper substrate 200A and a lower substrate 200B, the upper substrate 200A is implemented as an example of the above-mentioned first substrate, the lower substrate 200B is implemented as an example of the above-mentioned second substrate, the upper substrate 200A and the lower substrate 200B Bonded by the adhesive layer 200C. For example, the upper substrate 200A, the adhesive layer 200C and the lower substrate 200B have substantially the same shape, so that the adhesive layer 200C can better realize the bonding between the upper substrate 200A and the lower substrate 200B. For the materials of the upper substrate 200A, the lower substrate 200B, and the adhesive layer 200C, reference may be made to the above-mentioned embodiments, and details are not described herein again.

For example, the first channel 204 and the second channel 205 are provided in the upper substrate 200A. For example, the sample injection structure 201 and the sample detection structure 202 are also provided in the upper substrate 200A. At this time, the sample injection structure 101 can input the sample to be tested into the sample filtering structure 203 through the first opening 2032A from the side and the direction perpendicular to the plane of the filter membrane 2031, and the filtered sample to be tested can be injected from the side to the sample filter structure 203. , and output to the sample detection structure 102 through the second opening 1032B. Thereby, the functions of lateral chromatography and vertical liquid feeding of the sample filter structure 103 are realized.

For example, the sample detection structure 202 includes a plurality of detection units 2021 and a plurality of first detection flow channels 2022 , and at least one detection unit 2021 communicates with the sample filter structure 203 through a corresponding first detection flow channel 2022 . For the structure, size parameters and connection method of the first detection flow channel 2022, reference may be made to the above-mentioned embodiments, and details are not described herein again.

For example, the detection unit 2021 includes a detection chamber for accommodating the sample to be detected, and the detection chamber has an exhaust hole 2021A and a gas-permeable liquid-resistance film 2021B covering the exhaust hole 2021A. The gas permeable and liquid barrier film 2021B has the function of gas permeation but liquid impermeability, so as to prevent the sample to be tested from flowing out of the detection cavity.

For example, in some embodiments, the gas-permeable and liquid-blocking films 2021B of the plurality of detection units 2021 covering the exhaust holes 2021A are of an integral structure. In this case, the gas-permeable and liquid-blocking films 2021B of the integral structure may cover multiple The side of the detection unit 2021 with the exhaust hole 2021A (as shown in FIG. 13 ) can simplify the structure and manufacturing difficulty of the detection chip.

For example, in some embodiments, the sample injection structure 201 includes a sampling structure mounting portion 2011 for receiving, eg, mounting, a sampling structure. For example, the sample injection structure 201 further includes a reagent pool 2012 . After the sampling structure is installed in the sampling structure mounting portion 2011 , the sampling structure can communicate with the reagent pool 2012 , and the reagent pool 2012 can also communicate with the sample filter structure 203 .

For example, in some embodiments, the sample injection structure 201 may further include a first sealing layer 2013 on the first side (shown as the upper side in the figure) of the reagent cell 2012, and the first sealing layer 2013 is used on the first side Seal the reagent cell 2012. After the sampling structure is installed on the sampling structure mounting portion 2011 , the sampling structure can pierce the first sealing layer 2013 to communicate with the reagent pool 2012 .

For example, the sample injection structure 201 may further include a second sealing layer 2014 on the second side (shown as the lower side) of the reagent cell 2012, the second sealing layer 2014 is used to seal the reagent cell 2012 on the second side, and The first side of the reagent cell 2012 and the second side of the reagent cell 2012 are facing each other. After the second sealing layer 2014 is punctured, the reagent cell 2012 communicates with the sample filter structure 203 .

For example, in some embodiments, the sample injection structure 201 may further include an elastic membrane 2015 and an action channel 2016 on the second side of the reagent cell 2012 , and the second sealing layer 2014 is sandwiched between the elastic membrane 2015 and the reagent cell 2012 . The adhesive layer 100C is sandwiched between the elastic film 2015 and the second sealing layer 2014, thereby bonding and fixing the elastic film 2015 and the second sealing layer 2014 relative to each other. Meanwhile, referring to FIG. 13 , corresponding to the action channel 2016 , the adhesive layer 200C further has an opening 2001 , and the opening 2001 is also located in the area where the elastic film 2015 and the second sealing layer 2014 are facing and overlapped with each other. For example, the opening 2001 is completely located in the elastic film 2001 In the area where the film 2015 and the second sealing layer 2014 are facing each other and overlap each other, and the area where the elastic film 2015 and the second sealing layer 2014 are facing and overlapping each other are completely bonded and fixed by the adhesive layer 200C. The acting channel 2016 allows when an external force acts on the elastic film 2015 to deform the elastic film 2015, the deformed elastic film 2015 partially passes through the opening 2001 of the adhesive layer 200C, thereby enabling the elastic film 2015 itself to be punctured without being punctured. The external force also acts on the second sealing layer 2014 to puncture the second sealing layer 2014. After the external force is removed, the elastic membrane 2015 will basically return to its original state.

For example, the material of the elastic film 2015 can be a composite polymer material, such as a composite material of polystyrene (PS) and polyethylene terephthalate (PET). Therefore, the elastic film 2015 can have good elasticity and strength at the same time.

For example, the sampling structure mounting part 2011 and the reagent cell 2012 are provided on the upper substrate 200A, the adhesive layer 200C is attached to the surface (shown as the lower surface in the figure) of the upper substrate 200A, and the elastic film 2015 is fixed on the upper substrate 200A on the surface. For example, the lower substrate 200B includes a substrate opening to provide the active channel 2016 , eg, the elastic membrane 2015 completely covers the active channel 2016 .

For example, the adhesive layer 200C at least partially exposes the second sealing layer 2014 through the opening 2001 to allow the deformed elastic membrane 2015 to act on the second sealing layer 2014 to puncture the second sealing layer 2014 . The projection of the third opening 1001 of the adhesive layer 200C on the lower substrate 100B, for example, at least partially overlaps with the action channel 1016 , for example, completely within the action channel 1016 .

For example, in some embodiments, as shown in FIG. 12 , the detection chip 200 may further include a sample mixing chamber 206 disposed between the sample injection structure 201 and the sample filter structure 203 . The sample mixing chamber 206 can be used to mix the tested sample with the diluent in the reagent cell.

For example, in addition to the detection chip 200 described above, the detection system provided in this embodiment further includes a sampling structure 210 and a packaging structure 211 . The sampling structure 210 is mounted on the sampling structure mounting portion 2011 , and the packaging structure 211 is used for sealing the sampling structure 210 . For example, the encapsulation structure 211 is a silicone cap, so that it has better elasticity and tightness, and the sample can be mixed through the cooperation of the sampling structure 210 and the encapsulation structure 211 .

For example, in some embodiments, as shown in FIG. 12 , the package structure 211 includes a sealing part 211A and a fixing part 211B, the sealing part 211A is used to seal the sampling structure 210 , and the fixing part 211B is used to fix the sampling structure 210 to the detection chip 200 superior. For example, the sealing portion 211A and the fixing portion 211B are integrally formed with a silicone structure. The fixing structure 211B is an annular socket structure.

For example, the sealing portion 211A may also have structures such as a main body portion and an exhaust hole. For details, reference may be made to the packaging structure 111 provided in the above-mentioned embodiment, which will not be repeated here.

For example, in some embodiments, the detection system further includes a movable first push rod 220 , and the first push rod 220 is disposed on a side of the packaging structure 211 away from the sampling structure 210 . The first ejector pin 220 may apply a force to the sealing portion 211A of the packaging structure 211 .

For example, the detection system may further include a movable second plunger 230 for piercing the second sealing layer 2014 . For example, the second ejector pin 230 may apply a force to the second sealing layer 2014 through the action channel 2016 in the lower substrate 100B and the elastic mold 2015 .

Hereinafter, the working process of the above detection system will be exemplarily introduced with reference to FIG. 14 .

First, the sample to be detected is sucked by the sampling structure 210 . For example, the sampling structure 210 may be any structure with sampling function. The sample to be detected may be, for example, blood, body fluid, etc., which is not limited in the embodiments of the present disclosure.

Then, the sampling structure 210 is mounted on the detection chip 200 , and the sampling structure 210 is fixed and sealed by using the packaging structure 211 . For example, after the sampling structure 210 is installed on the detection chip 200 , the bottom of the sampling structure 210 extends into the reagent pool 2012 of the detection chip 200 . For example, the upper surface of the reagent pool 2012 has a first sealing layer 2013 for sealing. When the sampling structure 210 is installed on the detection chip 200 , the sampling structure 210 can pierce the first sealing layer 2014 to connect with the reagent pool 2012 so that the sample to be tested can be mixed with the diluent in the reagent cell 2012.

The second top rod 230 is driven to move upward, so that the second top rod 230 exerts force on the second sealing layer 2014 under the reagent cell 2012 through the acting channel 2016 and the elastic membrane 2015 to puncture the second sealing layer 2014 . Since the elastic film 2015 has elasticity, it can return to its original state after the external force is removed. After the second sealing layer 2014 is punctured, the reagent cell 1012 communicates with the sample mixing chamber 206 .

The first ejector rod 220 is used to apply a force to the sealing portion 211A of the sealing structure 211 at a first speed. At this time, the pressure inside the sampling structure 210 increases, so that the sample to be tested is pushed out of the sampling structure 210 and enters the reagent pool. In 2012, further, the tested sample and the diluent in the reagent cell 2012 can be mixed and can enter the sample mixing chamber 206.

After that, the first ejector rod 220 is withdrawn at the second speed. At this time, the sealing portion 211A rebounds, and the mixed solution of the diluent and the sample to be detected will be sucked back into the reagent pool 2012 . Thus, the mixture of the diluent and the sample to be tested can reciprocate in the reagent cell 2012 and the mixing chamber 206 to complete the mixing operation.

For example, the first speed at which the first ejector rod 220 exerts the force on the sealing portion 211A is lower than the second speed at which the first ejector bar 220 withdraws, that is, the first ejector bar 220 is slowly pressed down and raised quickly. Helps to improve the mixing effect of the tested sample and the diluent.

For example, the above-mentioned mixing operation can be performed for many times, so that the mixed liquid performs multiple reciprocating movements in the reagent tank 2012 and the mixing chamber 206, so as to further improve the mixing effect of the tested sample and the diluent.

For example, FIG. 14 shows the flow path of the sample to be detected in the detection chip 200 of the above-mentioned embodiment. As shown in FIG. 13 , the slow and continuous pressing operation of the first ejector rod 220 can continue to make the sample to be tested enter the sample filter structure 203 (the sample to be tested is input from the direction of the side and perpendicular to the plane of the filter membrane 2031 , for example, at the same time). into the filter membrane 2031), the filtered sample to be tested can be transported to the sample detection structure 202 during the continuous pressing of the first ejector rod 220 (the sample to be tested is output to the sample detection structure 202 from the side, for example), Finally, it is transported into the detection chamber 2021 of the sample detection structure 202 . For example, different detection chambers 2021 have freeze-dried reagents suitable for different detection items, so that after the sample to be detected can react with the freeze-dried reagent, the sample detection structure 202 starts to detect and outputs the detection results. For example, during the detection process of the sample detection structure 202, the first ejector pin 220 is always kept in a depressed state to avoid backflow of the sample to be detected.

Therefore, the detection system can realize an automatic detection process, and the detection system can also obtain more accurate detection results.

For example, in some embodiments, the detection system can also be used with a sampling structure with acquisition and mixing functions, for example, with the sampling structure shown in FIGS. 7 and 9-10 , for example, in some examples Used in conjunction with the sealing structure shown in Figures 8A and 8B. At this time, the detection system can also perform the detection operation through an operation process similar to that provided in the above-mentioned embodiment. The embodiments of the present disclosure do not specifically limit the operation process of the detection system.

At least one embodiment of the present disclosure provides yet another detection chip and a detection system, and the detection system includes the detection chip. For example, FIG. 15 shows an exploded top view of the detection system provided by this embodiment. The detection chip of the detection system of this embodiment is a modification of the detection chip and detection system shown in FIG. 12 and FIG. 13 , and the difference from the detection chip and detection system shown in FIG. 12 and FIG. 13 is that the detection chip only includes the upper substrate 300A and The lower substrate is not included, and the elastic membrane 3015 located on the second side of the reagent cell (the lower side in the figure) covers the lower surface of the upper substrate 300A in the figure, rather than just covering the reagent cell and its surrounding area, so that the elastic membrane 3015 also plays the role of the lower substrate in the detection chip shown in FIG. 12 and FIG. 13 . The same parts of the embodiment shown in FIG. 15 and the embodiments shown in FIG. 12 and FIG. 13 will not be repeated here.

For example, the material of the elastic film 3015 can be a composite polymer material, such as a composite material of polystyrene (PS) and polyethylene terephthalate (PET). Therefore, the elastic film 2015 can have good elasticity and strength at the same time.

The adhesive layer 300C is attached on the lower surface of the upper substrate 300A in the figure and fixes the elastic film 3015 on the lower surface of the upper substrate 300A, and the adhesive layer 300C also has an opening 3001 . The ejector rod can directly act on the elastic mold 3015, and exert a force on the sealing layer on the side of the reagent pool through the opening 3001 of the adhesive layer 300C.

There are a few more points to note:

(1) The accompanying drawings of the embodiments of the present disclosure only relate to the structures involved in the embodiments of the present disclosure, and other structures may refer to general designs.

(2) In the drawings for describing the embodiments of the present disclosure, the thicknesses of layers or regions are exaggerated or reduced for clarity, ie, the drawings are not drawn on actual scale.

(3) The embodiments of the present disclosure and the features in the embodiments may be combined with each other to obtain new embodiments without conflict.

The above are only specific embodiments of the present disclosure, but the protection scope of the present disclosure is not limited to this. should be included within the scope of protection of the present disclosure. Therefore, the protection scope of the present disclosure should be subject to the protection scope of the claims.

Claims (32)

1. A detection chip, comprising:

the sample injection structure is used for injecting a sample to be detected;

a sample detection structure for allowing the sample to be detected,

a sample filtering structure between and in communication with the sample injection structure and the sample detection structure, respectively, for filtering the injected sample in a lateral flow manner and transferring the filtered sample to the sample detection structure,

wherein the sample injection structure and the sample filtration structure are connected by a first channel and the sample filtration structure and the sample detection structure are connected by a second channel; the sample filtering structure comprises a filtering layer, and the first channel and the second channel are respectively connected to different layer surfaces of the filtering layer in a direction perpendicular to the plane of the filtering layer.

2. The detection chip according to claim 1,

the filter layer is configured to receive the detected sample from the sample injection structure at a first side, filter the detected sample in a plane along which the filter layer lies, and output the filtered detected sample at a second side opposite the first side.

3. The detection chip according to claim 2, wherein the first channel at least partially overlaps and interfaces with the filter layer in a direction perpendicular to a plane of the filter layer at the first side of the filter layer, so that the sample to be detected from the sample injection structure is injected into the filter layer for filtering.

4. The detection chip according to claim 3, wherein the second channel at the second side of the filter layer is at least partially overlapped with the filter layer in a direction perpendicular to the plane of the filter layer to receive the filtered sample to be detected;

wherein the second channel outputs the detected sample from the filter layer along the direction perpendicular to the plane of the filter layer.

5. The detection chip according to claim 2, wherein the sample filtration structure further comprises a filter chamber for accommodating the filter layer, the filter chamber comprising a first opening for inputting the sample to be detected and a second opening for outputting the filtered sample to be detected,

wherein the second opening is connected to a first end of the second channel and the sample detection structure is connected to a second end of the second channel.

6. The detection chip of claim 5, further comprising a first substrate and an adhesive layer, wherein the filter cavity is disposed on the first substrate, and the adhesive layer is attached to a surface of the first substrate and fixes the filter layer in the filter cavity.

7. The detection chip of claim 6, wherein the first channel and the second channel are disposed in the first substrate.

8. The detection chip of claim 7, wherein the sample injection structure and the sample detection structure are disposed on the first substrate.

9. The detection chip according to claim 6, further comprising a second substrate, wherein the second substrate is laminated with the first substrate and bonded by the adhesive layer,

the adhesive layer defines the first opening and the second opening of the filter cavity,

the first channel and the second channel are formed in the second substrate and communicate with the first opening and the second opening, respectively.

10. The detection chip of claim 9, wherein the sample injection structure and sample detection structure are disposed on the second substrate.

11. The detecting chip according to any one of claims 5 to 10, wherein the sample detecting structure includes a plurality of detecting units and a plurality of first detecting flow paths,

at least one detection unit is communicated with the sample filtering structure through a corresponding first detection flow channel;

at least one detection unit is communicated with the second end of the second channel through a corresponding first detection flow channel.

12. The detection chip of claim 11, wherein the first detection flow channel has a width of 0.1mm to 1mm and a depth of 0.1mm to 1 mm.

13. The detection chip of claim 11, wherein at least a portion of the first detection flow channel extends in a bent manner.

14. The detection chip of claim 13, wherein at least a portion of the first detection flow channel extending in a zigzag manner or an S-shape.

15. The detection chip according to claim 14, wherein at least a portion of the bent extension of the first detection flow channel includes 2 to 20 bends, and the length of the flow channel between two adjacent bends is 2mm to 20 mm.

16. The detecting chip of claim 15, wherein when at least a portion of the bent extension of the first detecting flow channel is in a zigzag shape, a bending angle of the bend is 5 ° to 120 °.

17. The detection chip according to claim 11, wherein the detection unit includes a detection chamber for accommodating the sample to be detected,

the detection cavity is provided with an exhaust hole and a ventilation liquid blocking film covering the exhaust hole.

18. The detection chip of any one of claims 1-10, wherein the sample injection structure comprises a sampling structure mount for receiving a sampling structure having the sample to be detected therein.

19. The detection chip of claim 18, wherein the sample injection structure further comprises a reagent reservoir,

when the sampling structure install in behind the sampling structure installation department, the sampling structure can with reagent pond intercommunication to

The reagent reservoir is also configured to be communicable with the sample filtration structure.

20. The detection chip of claim 19, wherein the sample injection structure further comprises a first sealing layer located on a first side of the reagent reservoir,

the first sealing layer is for sealing the reagent reservoir at the first side,

when the sampling structure install in behind the sampling structure installation department, the sampling structure can puncture first sealing layer in order with reagent pond intercommunication.

21. The detection chip of claim 20, wherein the sample injection structure further comprises a second sealing layer located at a second side of the reagent reservoir,

the second sealing layer is used for sealing the reagent pool at the second side, the first side of the reagent pool and the second side of the reagent pool are opposite to each other,

when the second sealing layer is punctured, the reagent reservoir is in communication with the sample filtration structure.

22. The detection chip of claim 21, wherein the sample injection structure further comprises an elastic membrane and an action channel on a second side of the reagent cell, the second sealing layer being interposed between the elastic membrane and the reagent cell,

the action passage allows an external force to act also on the second sealing layer to puncture the second sealing layer when the external force acts on the elastic film to deform the elastic film.

23. The detection chip of claim 22, wherein the material of the elastic membrane is a composite polymer material.

24. The detection chip according to claim 22, further comprising a first substrate and an adhesive layer, wherein the sampling structure mounting portion and the reagent reservoir are disposed on the first substrate, and the adhesive layer is attached on a surface of the first substrate and fixes the elastic membrane on the first substrate.

25. The detection chip of claim 24, wherein the adhesive layer at least partially exposes the second sealing layer to allow the deformed elastic film to act on the second sealing layer to puncture the second sealing layer.

26. The detection chip according to claim 24, further comprising a second substrate, wherein the second substrate is laminated with the first substrate and bonded by the adhesive layer,

the second substrate includes a substrate opening to provide the active channel.

27. The detection chip of any one of claims 1-10, further comprising a sample mixing chamber disposed between the sample injection structure and the sample filtration structure;

the sample mixing chamber is used for mixing the detected sample and a diluent.

28. An inspection system comprising the inspection chip of any of claims 1-27, a sampling structure, and a packaging structure,

the detection chip comprises a sampling structure installation part, the sampling structure is installed on the sampling structure installation part, and the packaging structure is used for sealing the sampling structure;

wherein the sampling structure is mounted to the sampling structure mounting portion through a gasket.

29. The detection system of claim 28, wherein the encapsulation structure is a silicone cap.

30. The detection system of claim 29, wherein the encapsulation structure includes a sealing portion and a securing portion,

the sealing part is used for sealing the sampling structure, and the fixing part is used for fixing the sampling structure on the detection chip.

31. The detection system of claim 30, further comprising a movable first push rod disposed on a side of the encapsulation structure distal from the sampling structure.

32. The detection system of claim 31, wherein the detection chip further comprises a reagent reservoir and a second sealing layer,

the detection system further comprises a second removable push rod for puncturing the second sealing layer.

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