CN112811753A

CN112811753A - Inhibitor and method for inhibiting escape of cow dung ammonia in cowshed

Info

Publication number: CN112811753A
Application number: CN202110268320.5A
Authority: CN
Inventors: 曹铭; 王文颖
Original assignee: Qinghai Normal University
Current assignee: Qinghai Normal University
Priority date: 2021-03-12
Filing date: 2021-03-12
Publication date: 2021-05-18

Abstract

一种抑制牛棚中牛粪氨逸散的抑制剂包括植物提取物35‑45份，芽孢杆菌25‑30份，放线菌15‑20份，乳酸菌8‑10份，蓝细菌6‑8份，酵母粉4‑8份，醋酸杆菌2‑4份，硝化细菌培养基10‑12份，明矾20‑25份，磷酸二氢钾20‑25份，水400‑600份。一种抑制牛棚中牛粪氨逸散的方法包括:将牛棚中散落的牛粪集中，将上述步骤中得到的溶液按1：150稀释，将稀释后的溶液装入喷淋装置中，每间隔6‑7天对牛粪进行喷淋，且每日喷洒3‑5次，喷淋时对牛粪进行搅拌。An inhibitor for inhibiting the escape of cow dung ammonia in a cowshed comprises 35-45 parts of plant extracts, 25-30 parts of bacillus, 15-20 parts of actinomycetes, 8-10 parts of lactic acid bacteria, and 6-8 parts of cyanobacteria , 4-8 parts of yeast powder, 2-4 parts of Acetobacter, 10-12 parts of nitrifying bacteria medium, 20-25 parts of alum, 20-25 parts of potassium dihydrogen phosphate, and 400-600 parts of water. A method for suppressing the escape of cow dung ammonia in the cow shed comprises: gathering the scattered cow dung in the cow shed, diluting the solution obtained in the above steps by 1:150, and loading the diluted solution into a spray device, The cow dung is sprayed every 6-7 days, and sprayed 3-5 times a day, and the cow dung is stirred during spraying.

Description

Inhibitor and method for inhibiting escape of cow dung ammonia in cowshed

Technical Field

The invention belongs to the technical field of livestock breeding, and particularly relates to an inhibitor and a method for inhibiting dissipation of cow dung ammonia in a cowshed.

Background

The ammonia gas in the cowshed is a toxic gas which causes serious harm to the physical and psychological health of livestock and feeding personnel, and the ammonia (NH3) discharged by the animal feeding is a main air and water pollutant which causes water eutrophication, soil acidity and aerosol formation. Ammonia can react with water in the respiratory tract of livestock and poultry to generate water-soluble corrosive NH4+ ions, further the respiratory tract mucosa system is damaged, the immunity of the livestock and poultry is reduced, so that the livestock and poultry are easily infected by germs, serious disasters are brought to livestock and poultry breeding, ammonia enters a blood circulation system through a respiratory system and is combined with hemoglobin, heme is converted into hematin, the oxygen carrying capacity of the hemoglobin, the blood alkali storage capacity and the oxidation performance of the heme are reduced, further the anemia and the tissue hypoxia of the livestock and poultry are caused, and the resistance of organisms to diseases is reduced. Studies have shown that high concentrations of ammonia in livestock houses can also have an effect on the growth of livestock animals, especially in their young growth period. How to reduce the cowshed ammonia concentration is always the important method of improving the cowshed environment, increasing the cowshed benefit and optimizing the working environment of workers, but the current method for treating ammonia by the cowshed has high cost and hard damage with high requirements on factory facility hardware, and how to use the environment-friendly mode of easily obtained raw materials to remove ammonia is always a technical problem troubling the workers. Therefore, an inhibitor and a method for inhibiting the escape of cow dung ammonia in a cowshed are provided.

Disclosure of Invention

The invention aims to provide an inhibitor and a method for inhibiting the escape of cow dung ammonia in a cowshed, so as to solve the problems in the prior art.

In order to solve the technical problems, the technical scheme adopted by the invention is as follows:

an inhibitor and a method for inhibiting the escape of cow dung ammonia in a cowshed are characterized in that: the composite material consists of the following raw materials in parts by mass: 35-45 parts of plant extract, 25-30 parts of bacillus, 15-20 parts of actinomycetes, 8-10 parts of lactic acid bacteria, 6-8 parts of cyanobacteria, 4-8 parts of yeast powder, 2-4 parts of acetobacter, 10-12 parts of nitrobacteria culture medium, 20-25 parts of alum, 20-25 parts of potassium dihydrogen phosphate and 600 parts of water 400-.

Further, the plant extract is an extract of Terminalia catappa leaves.

Further, the nitrobacteria culture medium comprises distilled water, peptone, sodium chloride 1 and yeast extract.

Further, the extraction method of the Terminalia catappa leaf extract comprises the following steps:

s1, screening Terminalia catappa leaf plants, selecting plants with regular germination, large density, the number of buds and leaves in a unit area and consistent bud and leaf maturity as Terminalia catappa leaf picking plants in 4 and 5 months each year;

s2, fence protection, namely picking the olive kernel leaves to plant for fence protection;

s3, picking up the olive kernel leaves, collecting the olive kernel leaves after the dry leaves of the olive kernel leaves naturally fall off a mother tree, naturally drying the olive kernel leaves in the air, and cutting the leaves;

s4, soaking crushed leaves, weighing 150 g of the Terminalia catappa leaf fragments obtained in the step S3, adding 10-15L of water, and soaking for 3-5 days;

s5, preparing the terminalia leaf extract, filtering the terminalia leaf in the step S4 by using a filter screen, further kneading, extracting for 2-5 times by using 20-80% ethanol, combining the extracting solutions, concentrating and drying.

Further, the preparation method of the nitrobacteria culture medium comprises the following steps:

l1, adding 10-20 g of peptone, 10-20 g of sodium chloride and 5-10 g of yeast extract into per liter of distilled water, putting into a stirrer, stirring for 20-35min, and controlling the temperature at 28-30 ℃;

and L2, adjusting the pH value of the solution obtained in the step L1 to 7 by using sodium hydroxide, sterilizing, adding the nitrifying bacteria mother solution in an aseptic operation, and carrying out shake culture at room temperature for 3-4 days.

Further, the method for inhibiting the escape of cow dung ammonia in the cowshed comprises the steps of collecting the cow dung scattered in the cowshed, and mixing the solution obtained in the step 1: 150, filling the diluted solution into a spraying device, spraying the cow dung at intervals of 6-7 days for 3-5 times every day, and stirring the cow dung during spraying.

Compared with the prior art, the invention has the beneficial effects that:

1. terminalia catappa leaves used in the invention are leaves of common tree species in the south, the rangooncreeper is used as a common street greening tree species, the common tree species can widely grow in the city of province in the south, fallen leaves are easy to obtain and the cost is extremely low, nitrobacteria are used as the strains of the traditional aquarium cleaning tank and are widely bought and sold in the market, the cultivation is simple, the cultivation cost is low, the cultivation period is extremely short, potassium dihydrogen phosphate is the most widely used agricultural fertilizer in agriculture, the price is extremely low, potassium dihydrogen phosphate is generally sold in the market at 5 yuan to 40 yuan per kilogram according to different qualities, and compared with the traditional ammonia removal agents such as urease inhibitor, activated carbon, ferrous sulfate and the like, the cost is lower.

2. Terminalia leaves are used as common fallen leaves of trees and are very easy to degrade in the natural environment, nitrobacteria are used as microorganisms commonly existing in nature and widely exist in soil and water body environment, and do not have toxic action on human and livestock, monopotassium phosphate is one of the most commonly used fertilizers, and human uses the fertilizer for more than one hundred years, so that the safety of the fertilizer is self-evident, and the pesticide can also increase the fertilizer efficiency of cow dung.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described with reference to the following examples:

example 1

An inhibitor for inhibiting the dissipation of cow dung ammonia in a cowshed is characterized in that: the composite material consists of the following raw materials in parts by mass: 35 parts of plant extract, 25 parts of bacillus, 15 parts of actinomycetes, 8 parts of lactic acid bacteria, 6 parts of cyanobacteria, 4 parts of yeast powder, 2 parts of acetobacter, 10 parts of nitrobacteria culture medium, 20 parts of alum, 20 parts of monopotassium phosphate and 400 parts of water, wherein the plant extract is an extract of terminalia leaves.

The extraction method of the Terminalia catappa leaf extract comprises the following steps:

The nitrobacteria culture medium comprises distilled water, peptone, sodium chloride 1 and yeast extract, and the preparation method of the nitrobacteria culture medium comprises the following steps:

A method for inhibiting the escape of cow dung ammonia in a cowshed comprises the steps of collecting cow dung scattered in the cowshed, and mixing the solution obtained in the steps according to a ratio of 1: 150, filling the diluted solution into a spraying device, spraying the cow dung at intervals of 6-7 days for 3-5 times every day, and stirring the cow dung during spraying.

Example 2

An inhibitor for inhibiting the dissipation of cow dung ammonia in a cowshed is characterized in that: the composite material consists of the following raw materials in parts by mass: 45 parts of plant extract, 30 parts of bacillus, 20 parts of actinomycetes, 10 parts of lactic acid bacteria, 8 parts of cyanobacteria, 8 parts of yeast powder, 4 parts of acetobacter, 12 parts of nitrobacteria culture medium, 25 parts of alum, 25 parts of potassium dihydrogen phosphate and 600 parts of water.

To demonstrate the effectiveness of this technique, experiments were conducted as follows: the fresh cow dung-urine mixture was divided into three groups of 4 replicates, each: the system comprises a non-treatment group, a NBPT urease inhibitor group and a novel medicament group, wherein each group repeatedly weighs 300 g of a mixture of excrement and urine, 200 ml of water is added into the non-treatment group, 200 ml of water and two g of urease inhibitor NBPT are added into the urease inhibitor group according to a literature method to simulate the use of an ammonia volatilization instrument by the inventor, 200 ml of medicament is added into the novel medicament group, an ammonia volatilization detector is used for continuously tracking and measuring data for 7 days, and the experimental result is as follows:

TABLE 1

As can be seen from Table 1: compared with a non-treatment group, the novel medicament group has remarkable effect, the ammonia volatilization amount is almost inhibited by 90% in the first day, the inhibition effect is slowly reduced in the later six days, but the ammonia volatilization amount is also inhibited by 30% even in the seventh day, and compared with a urease inhibitor, the effect in the first six days is basically the same as that in the seventh day, and the effect in the first six days is even remarkably superior to that in the urease inhibitor.

Claims

1. an inhibitor for suppressing the escape of cow dung ammonia in the cowshed is characterized in that: be made up of the raw materials of following parts by mass: 35-45 parts of plant extracts, 25-30 parts of bacillus, 15-30 parts of actinomycetes 20 parts, 8-10 parts of lactic acid bacteria, 6-8 parts of cyanobacteria, 4-8 parts of yeast powder, 2-4 parts of acetic bacteria, 10-12 parts of nitrifying bacteria medium, 20-25 parts of alum, 20 parts of potassium dihydrogen phosphate -25 parts, 400-600 parts water.

2 . The inhibitor of claim 1 , wherein the plant extract is a terminalia leaf extract. 3 .

3 . The inhibitor of claim 1 , wherein the nitrifying bacteria medium comprises distilled water, peptone, sodium chloride 1, and yeast extract. 4 .

4. a kind of inhibitor that suppresses the escape of cow dung ammonia in the cowshed according to claim 2, is characterized in that: the extraction method of described terminalia leaf extract comprises the following steps:

S1. Screening of Terminalia leaf plants, in April and May every year, plants with neat germination, large density of shoots and leaves per unit area, and the same maturity of shoots and leaves are selected as Terminalia leaf picking plants;

S2. Fence protection, fence protection of terminal tree leaf picking plants;

S3. Terminalia leaves are picked, and after the dry leaves of Terminalia leaves fall off the mother tree naturally, they are collected, then air-dried naturally, and the leaves are cut into pieces;

S4. Soak the broken leaves, weigh 100-150 grams of the terminal tree leaf pieces obtained in step S3, then add 10-15 L of water, and soak for 3 to 5 days;

S5. Prepare the terminalia leaf extract, filter out the terminalia leaf in step S4 with a filter screen, then further crush, extract with 20%-80% ethanol for 2-5 times, combine the extracts, concentrate and dry.

5. a kind of inhibitor that suppresses the escape of cow dung ammonia in the cowshed according to claim 3, is characterized in that: the preparation method of described nitrifying bacteria culture medium comprises the following steps:

L1. Add 10-20 grams of peptone, 10-20 grams of sodium chloride, and 5-10 grams of yeast extract per liter of distilled water, put them in a mixer and stir for 20-35 minutes, and control the temperature at 28-30 °C;

L2. Using sodium hydroxide to adjust the pH of the solution obtained in step L1 to 7, sterilizing, adding nitrifying bacteria mother liquor by aseptic operation, and culturing in a shaker at room temperature for 3-4 days.

6. A method for suppressing the escape of cow dung ammonia in the cow shed comprises: gathering the scattered cow dung in the cow shed, diluting the solution obtained in the above steps by 1:150, and loading the diluted solution into a spray device In the process, the cow dung is sprayed every 6-7 days, and sprayed 3-5 times a day, and the cow dung is stirred during spraying.