CN111543392A - A kind of Drosophila parasitoid large-scale rearing method and device - Google Patents

Abstract

The invention relates to the field of biological control of agricultural pests, and aims to provide a large-scale breeding method and device for fruit fly parasitoids. The method includes the steps of host inoculation and reproduction, Drosophila parasitoid inoculation and reproduction, Drosophila culture medium removal, Drosophila removal, parasitic wasp pupae collection and bee species preservation; The characteristics of flies, by controlling the feeding of Drosophila food, make the newly emerged Drosophila all die before the parasitoids emerge, and then separate the dead Drosophila adults. The present invention can separate the feed from the fruit flies, the fruit flies and the parasitic wasps through the feeding device; the generation overlapping phenomenon of the fruit flies is avoided, and the collection of the emerging parasitoid wasps in the later stage is facilitated. It is easy to operate, can standardize the feeding process, and is conducive to the large-scale reproduction of parasitic wasps. The invention can collect parasitic wasp pupae on a large scale and directly use it for large-scale release in the field.

Description

A kind of Drosophila parasitoid large-scale rearing method and device

technical field

The invention relates to the field of biological control of agricultural pests, in particular to a method and device for large-scale rearing of Drosophila parasitoids.

Background technique

The spotted-winged fruit fly (Drosophila suzukii Matsumura), also known as Suzuki's fruit fly, was first discovered in strawberry orchards in Yamanashi Prefecture, Japan. Different from common fruit flies, in addition to feeding on fallen fruit or damaged fruit, female adult flies can lay eggs directly on ripe or about to ripe cherries, peaches, European plums, grapes, strawberries, and raspberries. , blueberries, persimmons, tomatoes and other fruits with softer skins, larvae feed on the fruits, causing serious losses to the orchard. At present, the spotted-winged fruit fly has caused serious harm to the fruit industry in more than 30 countries around the world, including the United States, and has been listed as an important quarantine pest in many countries such as the United States, Japan, Australia, New Zealand, the United Kingdom, and Germany. In order to reduce the use of chemical pesticides, agricultural practitioners have expressed strong interest in sustainable control methods based on biological control. As an important natural enemy of Drosophila spotted-winged, parasitic wasps play an important role in the sustainable green control of D. spotted-winged Drosophila. Therefore, the research on the control ability of Drosophila parasitoid resources has received more and more attention.

At present, there are a variety of parasitic wasps known in the world that can parasitize fruit flies, about 4 families, 16 genera and more than 50 species, including Braconidae and Figitidae parasitic at the larval stage; Species of family Diapriidae and family Pteromalidae. In nature, Drosophila parasitoids can effectively control Drosophila populations with a parasitism rate of up to 90%. Therefore, parasitic wasps have great control potential for controlling Drosophila (especially Drosophila melanogaster).

Chinese invention patent CN102578052A discloses a kind of artificial rearing method of Drosophila melanogaster in bayberry garden and its pupal stage parasitic wasp pupa pupa A method for indoor reproduction of the larval parasitic wasp of the larval stage of Drosophila wasp and Drosophila melanogaster. The above disclosed patents can all realize the indoor small-scale breeding of Drosophila parasitoids, and the operation is simple and easy. However, in the process of using these technologies to propagate parasitoids on a large scale, it is still found that there are deficiencies: 1. Chinese invention patent CN102578052A utilizes worm cages to propagate flies and pupa golden bees. During the reproduction process of parasitic wasps, Drosophila pupae need to be collected separately and transferred to parasitic cages as parasitic sites. This step greatly increases the workload during large-scale propagation, and the cages are not conducive to the collection of adult parasitic wasps. 2. Chinese invention patents CN105794728A, CN107439491A and CN109169526A utilize Drosophila culture flasks to cultivate Drosophila and then multiply parasitic wasps. The parasitic and non-parasitic Drosophila develop together, and it is inevitable that the generation of Drosophila overlaps, which is not conducive to the collection of parasitoids that emerge later. At the same time, if the fruit fly feed is placed for a long time, it will become moldy and bacteria, and the humidity in the fruit fly culture bottle will greatly increase or even reach a viscous level, which is not conducive to the growth and development of parasitoids and will cause losses to the collection of parasitoids.

SUMMARY OF THE INVENTION

The technical problem to be solved by the present invention is to overcome the deficiencies of the prior art and provide a method and device for large-scale rearing of Drosophila parasitoids.

For solving the technical problem, the solution of the present invention is:

A method for large-scale rearing of Drosophila parasitoids is provided, comprising the steps of:

(1) Host inoculation and reproduction

Put Drosophila food in the breeding and propagation device, and transfer the adult Drosophila that has emerged for about a week and fully mated into it; move it to a constant temperature insect breeding room at 25 ± 1 °C for cultivation, and take out the Drosophila after 1 day of egg laying; Move the breeding and propagation device with Drosophila eggs to a constant temperature worm breeding room at 25±1°C and RH of 50% to continue culturing, and observe and record the number and development of Drosophila eggs;

(2) Drosophila parasitoid inoculation and reproduction

When the fruit fly eggs develop to the inoculation-appropriate age in step (1), according to the ratio of the number of female bees and fruit fly eggs 1: 10, select the parasitic wasp population and transfer it into the breeding and propagation device, and add parasitic wasp food simultaneously; observe; Parasitic wasps on Drosophila larvae or pupae, take out the parasitic wasps after 1 day of parasitisation;

(3) Drosophila medium removal

Move the breeding and propagation device to a constant temperature worm breeding room at 25±1°C and RH of 50% to continue culturing, and remove the fruit fly food after the fruit fly larvae develop into the pupal stage;

(4) Drosophila removal

Under the conditions of 25±1℃ and 50% RH, the parasitized Drosophila parasitoids will emerge 6-8 days later than normal Drosophila flies, and the unparasitized Drosophila flies will emerge first. Due to the removal of the fruit fly food, the newly emerged fruit flies will all die before the parasitoids emerge, and the dead fruit fly adults are removed;

(5) Collection of parasitic wasp pupae

Collect the parasitized Drosophila pupae before the parasitic wasps emerge, store them in the breeding and propagation device, and move them to a constant temperature incubator at 16±1°C and RH50%;

(6) Bee species preservation

About 18 days after parasitism, the parasitoid wasps emerge, use CO ₂ to anesthetize the adult parasitoids, and then quickly collect the adult bees, put them in a culture bottle containing parasitoid food, and place them in a culture of 16±1°C and RH 50%. Store in the box for inoculation in the next breeding.

In the present invention, the parasitic wasp is Drosophila larvae or Drosophila pupal stage; the Drosophila larval parasitoid is Leptopilina or Asobara; The parasitic wasps in the pupal stage of the fly are Spalangia, Pachycrepoideus or Trichopria.

In the present invention, in the step (2), when the parasitic wasps are inoculated with the fruit flies, it is necessary to ensure that the parasitic wasps are in the eclosion stage; for the fruit fly larvae parasitic wasps, the inoculation-appropriate age of the fruit flies is the larvae 1-2 instars; For the parasitic wasps in the pupal stage of Drosophila, the inoculation-appropriate age of the Drosophila is 1-2 days in the pupal stage.

In the present invention, the preparation method of the fruit fly food is as follows: take 120 g of dry yeast, dissolve it in 1000 ml of warm water, weigh 318.75 g of corn flour, 57 g of agar, 60 g of peptone, 90 g of brown sugar, 180 g of glucose, and 60 g of yeast extract, add 5000 ml of Stir and mix well in warm water, add dissolved 1000ml yeast liquid, heat and stir; when the mixture is cooked, add 1.5g MgSO ₄ ·7H ₂ O and 1.5g CaCl ₂ ·2H ₂ O in turn, and cool naturally after boiling To 50-60 ℃, add 180 ml of methyl paraben solution and 50 ml of propionic acid, stir evenly, after cooling for 6-7 hours, seal with gauze, put it in a 4 ℃ refrigerator for refrigeration; the methyl paraben solution is 100g of methylparaben was dissolved in 900ml of 95% ethanol.

In the present invention, the preparation method of the parasitic wasp food is as follows: take 27g of agar and add it to 1000ml of water, fully stir and mix, sterilize with high temperature steam at 120°C, then add 33g of brown sugar and 330ml of pure apple juice, fully stir and mix, and then naturally Cool to 60°C, add 20ml of methylparaben solution, stir and mix well in the cooled mixture, refrigerate at 4°C for later use; the methylparaben solution is 100g methylparaben dissolved in 900ml 95 % ethanol.

In the present invention, the fruit fly is Drosophila melanogaster, Drosophila melanogaster D. suzukii, or Drosophila melanogaster D. virilis.

The present invention further provides a breeding and propagation device used in the aforementioned method for large-scale breeding of Drosophila parasitoids, comprising a container body; an upper cover is arranged on the top of the container body, and a detachable bottom box is arranged at the bottom; the upper cover is provided with a ventilation A sponge plug is filled in the ventilation hole; inside the container body, there is a box body for containing the food of parasitic wasps;

The breeding and multiplying device also includes a fly pupae collection box that can be fitted with the bottom of the container body, the bottom ventilation hole is provided with a pull cover, and the upper opening is provided with a gauze. The aperture size of the gauze mesh can be 20-40 meshes according to the size of the adult parasitoid wasps.

In the present invention, the container body is in the shape of a cylinder or a cube, and the bottom box and the fly pupae collection box have shapes adapted to them.

In the present invention, the box body containing the parasitoid food is hung under the upper cover through a string; or installed on the container wall through a telescopic rail.

In the present invention, the bottom box and the fly pupae collection box are installed on the bottom of the container body by means of screw connection or snap fit.

Compared with the prior art, the beneficial effects of the present invention are:

1. The present invention can separate the feed from fruit flies, fruit flies and parasitic wasps through the feeding device; avoid the phenomenon of overlapping generations of fruit flies, and is conducive to the collection of parasitic wasps that emerge later.

2. The present invention is convenient to operate, can standardize the feeding process, and is beneficial to the large-scale reproduction of parasitic wasps.

3. The present invention can collect parasitic wasp pupae on a large scale, which can be directly used for large-scale release in the field.

Description of drawings

FIG. 1 is a schematic structural diagram of the Drosophila parasitic wasp multiplication device in the present invention.

FIG. 2 is a schematic diagram of the usage mode of the Drosophila parasitoid collection device in the present invention.

Reference numerals: 1 container body; 2 ventilation hole; 3 sponge plug; 4 string; 5 box body; 6 fixed collar; 7 bottom box; 8 fly pupae collection box; 9 gauze net; 10 pull cover.

Specific implementation cases

Below in conjunction with the accompanying drawings, the specific embodiments of the present invention will be further described in detail through the description of the implementation cases, so as to help those skilled in the art to have a more complete, accurate and in-depth understanding of the technical solutions of the invention.

The breeding and propagation device according to the present invention is shown in Figures 1 and 2, including a cylindrical or cubic container body 1 (cylindrical in the figure), the top of which is provided with an upper cover, and the bottom is provided with a detachable bottom box 7; The upper cover is provided with ventilation holes 2, and the ventilation holes are filled with sponge plugs 3; inside the container body 1, there is a box body 5 for containing food for parasitic wasps (the box body 5 has small holes, and the small holes are convenient for the parasitic wasps to eat), the box The body 5 is hung on the fixing collar 6 provided under the upper cover through the string 4, or is installed on the container wall through the telescopic rail. The device also includes a fly pupae collection box 8 that can be fitted with the bottom of the container body 1 , the bottom vent hole is provided with a pull cover 10 , and the upper opening is provided with a gauze 9 . The bottom box 7 and the fly pupae collection box 8 have shapes adapted to the container body 1, and can be installed on the bottom of the container body 1 by means of screw connection or snap fit. The cover 10 is used for picking and placing the fruit fly pupae, and the gauze 9 is used for separating the fruit fly pupae and emerging parasitoids.

When in use, the box body 5 is used to hold the food of parasitic wasps; the bottom box 7 is used to hold the food of fruit flies, and can also be removed and replaced with a new bottom box without medium to remove the food of fruit flies. The bottom box 7 is used for the process of inoculation and reproduction of Drosophila parasitoids, while the fly pupae collection box 8 is used for inoculation and reproduction of Drosophila parasitoids, and the parasitoids are collected. By combining with the container body 1 , the collected fruit fly pupae are put into the fly pupae collection box 8 by pulling the cover 10 . The emergent parasitoid quickly passes through the gauze 9 (20-40 mesh gauze is selected according to the size of the adult parasitoid parasitoid), and is separated from the fruit fly pupae in the fly pupae collection box 8 to achieve the purpose of rapidly collecting the emergent parasitoid.

Example 1: Drosophila larval parasitic wasp mass expansion

(1) Host inoculation and reproduction:

(A) Drosophila food preparation: Take 120g of dry yeast, dissolve it in 1000ml warm water, weigh 318.75g of corn flour, 57g of agar, 60g of peptone, 90g of brown sugar, 180g of glucose, and 60g of yeast extract, add them to 5000ml of warm water and mix them well. Add 1000ml of dissolved yeast liquid, heat and stir; when the mixture is cooked, add 1.5g MgSO ₄ ·7H ₂ O and 1.5g CaCl ₂ ·2H ₂ O in turn, and cool to 50-60°C after boiling, add 180ml methylparaben solution (100g methylparaben dissolved in 900ml 95% ethanol) and 50ml propionic acid, stir evenly, cool for 6-7h, seal with gauze, and put in a 4°C refrigerator for cold storage.

(B) Loading Drosophila food into the bottom box 7; taking 1000 adult Drosophila melanogaster that have emerged for about a week and fully mated, anesthetized with CO ₂ and transferred to the breeding and propagation device, and moved to a constant temperature of 25 ± 1 °C Drosophila was taken out after 1 day of egg laying in the insect house;

(C) The breeding and propagation device with Drosophila eggs in step (B) is placed in a constant temperature insect breeding room at 25±1° C. and RH of 50%, and the number and development of Drosophila eggs are observed and recorded.

(2) Inoculation and reproduction of parasitic wasps

(A) Parasitoid food configuration: add 27g of agar to 1000ml of water, stir and mix well, sterilize with high-temperature steam at 120°C, then add 33g of brown sugar and 330ml of pure apple juice, stir and mix well, cool to 60°C naturally, add 20ml of methylparaben solution (100g of methylparaben dissolved in 900ml of 95% ethanol), in the cooled mixture, fully stirred and mixed, and refrigerated at 4°C for later use.

(B) larval parasitoid inoculation and reproduction: after the Drosophila eggs hatch for 1-2 days in step (1), select the Drosophila larvae parasitoids preserved in the laboratory according to the ratio of 1:10 between female bees and Drosophila eggs, transfer to Into the Drosophila parasitoid propagation device.

When inoculating parasitic wasps, it is necessary to ensure that the parasitoid wasps are in the eclosion stage, and for larval parasitoids, ensure that the fruit fly instar stage is in the 1-2 instar larval stage.

(C) In order to improve the parasitic rate, the parasitic wasp food of step (A) was added in the box body 5 of the fruit fly parasitic wasp multiplication device as a nutritional supplement, and the parasitic wasps were observed to parasitize the fruit fly larvae, and the parasitic wasps were taken out after 1 day.

(3) Drosophila medium removal

Drosophila larvae have a Wandering stage before pupation, that is, the third instar larvae wander on the bottle wall and finally pupate on the bottle wall. According to this biological characteristic, the parasitized Drosophila larvae in step (2) are first placed in a constant temperature worm breeding room at 25±1° C. and RH of 50%, until the Drosophila larvae enter the pupal stage in step (2). Afterwards, the bottom box 7 with Drosophila food was directly replaced with a clean bottom box 7 (to avoid generation overlap of Drosophila in the next step).

(4) Drosophila removal

Under normal circumstances, it takes about 10 days for Drosophila to go through the entire developmental period of egg-larva-pupa-adult at 25°C. After parasitic wasps parasitize Drosophila, it will prolong the host development period. Under the condition of 25±1℃ and 50% RH, the eclosion of parasitic wasps of parasitized Drosophila was 6-8 days later than that of normal Drosophila. Therefore, before the parasitic wasps emerge, the fruit flies that have not been successfully parasitized will emerge first. Since the fruit fly food is removed in step (3), the newly emerged fruit flies have no food source, resulting in all of them dying before the parasitoids emerge. About 14 days after infestation, the dead fruit fly adults were removed.

(5) Collection of parasitic wasp pupae

With a soft brush, the parasitic fruit fly pupa in step (4) is scraped away from the side wall of the breeding and multiplying device, and it is quickly collected in the fly pupa collection box 8 (can be stored at 16 ± 1 ℃, RH 50% constant temperature. Reserve in the box for subsequent direct use in the field for large-scale release).

(6) Bee species preservation

(A) The fly pupae collection box 8 that has collected the parasitic wasp pupae in step (5) is fixed on the bottom of the container body, and after the parasitoid has emerged, utilize _CO to anesthetize the parasitoid into a bee, and quickly collect the emergent bee.

(B) Put the collected parasitic wasps into a culture bottle containing parasitic wasp food, and store the culture bottle in an incubator at 16±1°C and RH50% for the next propagation and inoculation.

In this example, Drosophila melanogaster was used, but D. suzukii, D. virilis, or other Drosophila species of the genus Drosophila can also be used. Drosophila larval parasitoids can be selected from Leptopilina or Asobara.

Example 2: Large-scale proliferation of parasitic wasps in the pupal stage of Drosophila

(1) Host inoculation and reproduction:

(A) Drosophila food (the same formula as in Example 1) was loaded into the bottom box 7; 1000 adult Drosophila melanogaster that had emerged for about a week and fully mated were taken and transferred to the breeding and propagation device after anesthesia with _CO medium, the flies were removed after 1 day of egg laying in a 25±1°C incubator;

(C) Transfer the breeding and propagation device with Drosophila eggs in step (B) to a constant temperature incubator at 25±1° C. and a RH of 50% for cultivation, and observe and record the number and development of Drosophila eggs.

(2) Inoculation and reproduction of parasitic wasps

(A) pupal stage parasitic wasp inoculation and reproduction: in step (1), fruit fly enters pupal stage for 1-2 days, according to the ratio of female bee and fruit fly 1: 10, selects the fruit fly pupal stage parasitic wasp of laboratory preservation , transferred to the breeding and breeding device.

When inoculating parasitic wasps, it is necessary to ensure that the parasitic wasps are in the eclosion stage. For parasitic wasps in the pupal stage, ensure that the parasitic fruit flies are Drosophila pupae for 1-2 days.

(B) in order to improve the parasitic rate, should add parasitic wasp food (the same formula as in Example 1) as nutritional supplement in the box body 5 of the breeding expansion device, observe that the parasitic wasp is parasitized to the pupa of Drosophila, take out after 1 day Parasitic wasp.

(3) Drosophila medium removal

After step (2) is completed, take out the fruit fly food in the bottom box 7, or directly replace the bottom box 7 with the fruit fly food with a clean bottom box 7 (avoid generation overlap of fruit flies in the next step) .

(4) Drosophila removal

Under normal circumstances, it takes about 10 days for Drosophila to go through the entire developmental period of egg-larva-pupa-adult at 25°C. After the parasitoid parasitizes Drosophila, it will prolong the development period of the host, so the time for the parasitoid to emerge is about 15-18 days after parasitism. Under the condition of 25±1℃ and 50% RH, the eclosion of parasitic wasps of parasitized Drosophila was 6-8 days later than that of normal Drosophila. Therefore, before the parasitic wasps emerge, the fruit flies that have not been successfully parasitized will emerge first. Since the fruit fly food is removed in step (3), the newly emerged fruit flies have no food source, resulting in all of them dying before the parasitoids emerge.

Dead fruit fly adults were removed 5-6 days after parasitism.

(5) Collection of parasitic wasp pupae

Utilize a soft brush to scrape the parasitic Drosophila pupa from the side wall of the culture device in step (4), and quickly collect it in a fly pupa collection box 8 (can be stored at 16±1° C., RH 50% in an incubator for subsequent use) , so that it can be directly used in the field for large-scale release).

(6) Bee species preservation

(A) The fly pupae collection box 8 that has collected the parasitic wasp pupae in step (5) is fixed at the bottom of the container body, and after the parasitoid wasp emerges, CO ₂ is used to numb the parasitoid wasp and get drunk into a bee, and quickly collect the bee that emerges.

(B) Put the collected parasitic wasps into a culture bottle containing parasitic wasp food, and store the culture bottle in an incubator at 16±1°C and RH50% for the next propagation and inoculation.

In this example, Drosophila melanogaster was used, but D. suzukii, D. virilis, or other Drosophila species of the genus Drosophila can also be used. Drosophila pupal stage parasitoids can be selected from the genus Spalangia, Pachycrepoideus or Trichopria.

Claims (10)

1. a Drosophila parasitoid large-scale rearing method, is characterized in that, comprises the following steps: (1) Host inoculation and reproduction Put Drosophila food in the breeding and propagation device, and transfer the adult Drosophila that has emerged for about a week and fully mated into it; move it to a constant temperature insect breeding room at 25 ± 1 °C for cultivation, and take out the Drosophila after 1 day of egg laying; Move the breeding and propagation device with Drosophila eggs to a constant temperature worm breeding room at 25±1°C and RH of 50% to continue culturing, and observe and record the number and development of Drosophila eggs; (2) Drosophila parasitoid inoculation and reproduction When the fruit fly eggs develop to the inoculation-appropriate age in step (1), according to the ratio of the number of female bees and fruit fly eggs 1: 10, select the parasitic wasp population and transfer it into the breeding and propagation device, and add parasitic wasp food simultaneously; observe; Parasitic wasps on Drosophila larvae or pupae, take out the parasitic wasps after 1 day of parasitisation; (3) Drosophila medium removal Move the breeding and propagation device to a constant temperature worm breeding room at 25±1°C and RH of 50% to continue culturing, and remove the fruit fly food after the fruit fly larvae develop into the pupal stage; (4) Drosophila removal Under the conditions of 25±1℃ and 50% RH, parasitized Drosophila parasitoids will emerge 6-8 days later than normal Drosophila; Drosophila that has not been successfully parasitized will emerge first; due to the removal of Drosophila food, new The eclosion fruit flies will all die before the parasitic wasps emerge, and the dead fruit fly adults will be removed; (5) Collection of parasitic wasp pupae Collect the parasitized Drosophila pupae before the parasitic wasps emerge, store them in the breeding and propagation device, and move them to a constant temperature incubator at 16±1°C and RH 50%; (6) Bee species preservation About 18 days after parasitism, the parasitoid wasps emerge, use CO ₂ to anesthetize the adult parasitoids, and then quickly collect the adult bees, put them in a culture bottle containing parasitoid food, and place them in a culture of 16±1°C and RH 50%. Store in the box for inoculation in the next breeding. 2. The method according to claim 1, wherein the parasitic wasp is a Drosophila larvae parasitoid or a Drosophila pupal stage parasitoid; wherein, the Drosophila larvae parasitoid is Leptopilina Or the parasitoid of the genus Asobara; the parasitoid in the pupal stage of Drosophila is the genus Spalangia, the genus Pachycrepoideus or the genus Trichopria. 3. method according to claim 1, is characterized in that, in described step (2), when parasitic wasp inoculates fruit flies, it is to ensure that parasitic wasp is in eclosion stage; For fruit fly larvae parasitic wasp, described fruit fly The inoculation-appropriate instar period is 1-2 instars of larvae; for Drosophila pupal stage parasitoids, the Drosophila inoculation-appropriate instar period is 1-2 days in the pupal stage. 4. method according to claim 1, is characterized in that, the compound method of described fruit fly food is: get dry yeast 120g, dissolve with 1000ml warm water, take by weighing corn flour 318.75g, agar 57g, peptone 60g, brown sugar 90g Add 180g of glucose, 60g of yeast extract into 5000ml of warm water, stir and mix well, add 1000ml of dissolved yeast liquid, heat and stir; when the mixture is cooked, add 1.5g of MgSO ₄ ·7H ₂ O and 1.5g of CaCl in turn ₂ 2H ₂ O, boiled and cooled to 50-60 ℃ naturally, add 180 ml of methyl paraben solution and 50 ml of propionic acid, stir well, after cooling for 6-7 hours, seal with gauze, and put it in a 4 ℃ refrigerator to refrigerate for later use; The methylparaben solution is prepared by dissolving 100g of methylparaben in 900ml of 95% ethanol. 5. method according to claim 1, is characterized in that, the compound method of described parasitic wasp food is: get 27g agar and add 1000ml water, after fully stirring and mixing, 120 ℃ of high temperature steam sterilization, then add 33g brown sugar and 330ml of pure apple juice, fully stirred and mixed, then cooled to 60°C naturally, added 20ml of methylparaben solution, the cooled mixture, fully stirred and mixed, and refrigerated at 4°C for later use; the methylparaben solution is It is prepared by dissolving 100g methylparaben in 900ml 95% ethanol. 6. The method of claim 1, wherein the fruit fly is Drosophila melanogaster, Drosophila melanogaster, D. suzukii, or D. virilis. 7. The breeding and multiplying device used for the large-scale breeding method of Drosophila parasitoids according to claim 1, comprising a container body; it is characterized in that, an upper cover is arranged on the top of the container body, and a detachable bottom box is arranged at the bottom; Cover with ventilation holes, and the ventilation holes are filled with sponge plugs; inside the container body, there is a box body for containing parasitic wasp food; The breeding and multiplying device also includes a fly pupae collection box that can be fitted with the bottom of the container body, the bottom ventilation hole is provided with a pull cover, and the upper opening is provided with gauze; Adult bee body size selection. 8 . The breeding and propagation device according to claim 1 , wherein the container body is in the shape of a cylinder or a cube, and the bottom box and the fly pupae collection box have shapes adapted to the container body. 9 . 9 . The breeding and multiplying device according to claim 1 , wherein the box body containing the food for parasitic wasps is hung under the upper cover through a string; or is installed on the container wall through a telescopic rail. 10 . 10 . The breeding and propagation device according to claim 1 , wherein the bottom box and the fly pupae collection box are installed on the bottom of the container body by means of screw connection or snap fit. 11 .

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