CN111011665A - Microbial fermentation wet material for promoting growth of aquatic animals and preparation method and application thereof - Google Patents
Microbial fermentation wet material for promoting growth of aquatic animals and preparation method and application thereof Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/33—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from molasses
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention provides a preparation method of a microbial fermentation wet material for promoting aquatic animal growth, which mainly comprises the following fermentation steps: mixing fermentation raw materials with the fermentation microorganisms, and performing solid state fermentation at 30 ℃ for 72 hours, wherein the mass of the fermentation microorganisms accounts for 0.3-0.5% of the mass of the fermentation raw materials. The content of live bacteria in the fermented feed reaches 100 hundred million/g, the water content is kept between 35 and 42 percent, and the proportion of mixed bacteria is less than or equal to 0.4 percent. The invention has the functions of regulating the balance of intestinal flora of aquatic animals, providing nutrition for organisms, inhibiting the growth of pathogenic bacteria, stimulating the immune response of the organisms, enhancing the anti-stress capability of the organisms, regulating water quality, reducing pollution, reducing harmful substances and the like, can promote the growth of the aquatic animals, has obvious effect and greatly increases the economic benefit.
Description
Technical Field
The invention relates to the field of biological feed additives, in particular to a microbial fermentation wet material for promoting the growth of aquatic animals and a preparation method and application thereof.
Background
In recent years, with the national consumption level and the continuous improvement of the quality of life, the aquatic animals with delicious taste and rich nutrition have larger and larger proportion in market consumption, the yield of the aquatic animals is continuously increased, and the aquaculture area is continuously expanded. Feed is the most important ingredient in the breeding industry, and feed additives are indispensable parts in feed. The safe, efficient, environment-friendly and healthy additive can promote aquatic animals to grow rapidly, can improve meat quality, improve flavor, shorten fishing time, reduce breeding risks and increase economic benefits, has important influence on both the aquaculture industry and the feed industry, and is an important material premise for realizing feed industrial production and breeding modernization.
Under the condition, the microbial feed additive gradually enters the visual field of people, which completely meets the requirements of safety, high efficiency, health and environmental protection, and has been gradually raised in the aquaculture industry. The microbial fermented feed is feed or raw material which is beneficial to animal feeding and utilization and is rich in high-activity probiotics and metabolites and is produced by decomposing or converting macromolecular substances and anti-nutritional factors in the feed through the metabolic activity of microbes under the condition of artificial control.
Beneficial microorganisms in the microbial fermented feed play an important role, and a plurality of feed additives are added into the feed in a powdery form, are uniformly stirred and are fed, or are added into water for animals to drink, or the microbial additives are smeared on the surface layer of the feed for feeding. Under the conditions, the number and the activity of viable bacteria of beneficial microorganisms are greatly reduced, so that the effect is seriously reduced. How to prepare the microbial fermented feed which can effectively keep the number and the activity of viable bacteria of beneficial microorganisms in the fermented feed, is green, safe, pollution-free and residue-free, and is suitable for the ingestion and absorption of aquatic animals is worth researching.
Disclosure of Invention
The invention overcomes the defects in the prior art, provides the wet microbial fermentation material for promoting the growth of aquatic animals, accords with the concepts of green safety, health and environmental protection, keeps the humidity of the additive on the basis of microbial fermentation, effectively keeps the quantity and activity of viable bacteria of beneficial microbes, plays the role to the maximum extent and improves the economic benefit of cultivation. The invention also aims to provide a preparation method of the wet microbial fermentation material for promoting the growth of aquatic animals, and the application of the wet microbial fermentation material for promoting the growth of aquatic animals in the field of feed additives.
The purpose of the invention is realized by the following technical scheme.
A wet microbial fermentation material for promoting the growth of aquatic animals comprises a fermentation raw material and the fermentation microbes, wherein the mass of the fermentation microbes accounts for 0.3-0.5% of the mass of the fermentation raw material.
Further, the fermentation raw material is prepared by mixing the following components in parts by weight: 43-53 parts of rice bran, 25-35 parts of soybean meal, 15-25 parts of bran, 1.5-2 parts of honey, 0.05-0.10 part of xylanase, 0.01-0.03 part of pectinase, 0.01-0.03 part of acid protease and 70 parts of water.
Further, the enzyme activity of the fermentation raw material is as follows: the xylanase is more than or equal to 30000u/g, the pectinase is more than or equal to 10000u/g, the acid protease is more than or equal to 60000u/g, and the cellulase is more than or equal to 30000 u/g.
Further, the fermentation microorganism is prepared by mixing the following components in parts by weight: 30-40 parts of saccharomyces cerevisiae, 9-11 parts of bacillus subtilis, 20-30 parts of lactobacillus acidophilus, 15-25 parts of lactobacillus plantarum, 5-15 parts of clostridium butyricum and 5 parts of honey.
Further, the viable count of the fermentation microorganism is as follows: the saccharomyces cerevisiae is more than or equal to 1000 hundred million CFU/g, the bacillus subtilis is more than or equal to 1500 hundred million CFU/g, the lactobacillus acidophilus is more than or equal to 1000 hundred million CFU/g, the lactobacillus plantarum is more than or equal to 1000 hundred million CFU/g, and the clostridium butyricum is more than or equal to 1000 hundred million CFU/g.
A preparation method for producing a microbial fermentation wet material for promoting the growth of aquatic animals comprises the following steps:
step one, strain activation: proportionally placing the fermented microorganisms into a clean sterilization container, adding appropriate activated water, mixing, and culturing in a 30-37 deg.C incubator for 30 min;
step two, liquid state fermentation: placing the fermentation raw material in a liquid fermentation tank, heating to 40 ℃ for enzymolysis for 40min, heating again to 70 ℃ for sterilization for 30min, rapidly cooling to 30 ℃, uniformly mixing the fermentation microorganism and the fermentation raw material in the step one, and performing solid fermentation for 72 hours at 30 ℃;
step three, checking and packaging: detecting the pH value, the moisture content and the viable count of the product, and bagging the product after the detection is qualified to prepare a finished product of the wet microbial fermentation material for promoting the growth of aquatic animals.
Further, the fermentation microorganism in the first step is prepared by mixing the following components in parts by weight: the fermentation microorganism comprises 30-40 parts of saccharomyces cerevisiae, 9-11 parts of bacillus subtilis, 20-30 parts of lactobacillus acidophilus, 15-25 parts of lactobacillus plantarum, 5-15 parts of clostridium butyricum and 5 parts of honey according to a proportion.
Further, the fermentation raw material in the step two is prepared by mixing the following components in parts by weight: 43-53 parts of rice bran, 25-35 parts of soybean meal, 15-25 parts of bran, 1.5-2 parts of honey, 0.05-0.10 part of xylanase, 0.01-0.03 part of pectinase, 0.01-0.03 part of acid protease and 70 parts of water.
Application of wet microbial fermentation material for promoting aquatic animal growth in the field of feed additive.
Furthermore, the addition amount of the fermented wet material is 20kg per ton of feed.
The invention has the beneficial effects that:
regulating the balance of intestinal flora of aquatic animals, and inhibiting the growth of pathogenic bacteria: functional substances such as probiotics, organic acid and the like are continuously supplemented into the intestinal tract, so that the quantity of beneficial bacteria in the intestinal tract is increased, the growth of pathogenic bacteria is inhibited, the quantity of harmful bacteria such as vibrios in the intestinal tract is reduced, the advantages of the beneficial bacteria in the intestinal tract are enhanced, the microecological balance of a digestive system is maintained, and the occurrence of intestinal tract diseases is reduced. Therefore, when the feed is fed for a long time, the intestinal tracts of aquatic animals are thicker, and the feed has a certain inhibition effect on bacterial enteritis.
Promoting the growth of organisms: the invention adopts the live bacteria for fermentation, has obvious natural fermentation acid fragrance, and generates lactic acid and yeast aromatic fat phagostimulant substances in the fermentation process, thereby having good food calling effect and greatly promoting the ingestion of aquatic animals. And macromolecular substances and anti-nutritional factors in the feed are decomposed or converted through the metabolic activity of microorganisms, so that the feed is more beneficial to animal feeding and digestion, and the effect of promoting growth is achieved.
Enhancing the immunity and anti-stress capability of the organism: biological enzymes, bacteriostatic active peptides, organic acids, phenolic antioxidant active substances, polysaccharides, growth promoting factors, multi-dimensional multi-minerals and the like released by metabolism of various beneficial bacteria in the fermented wet material have strong probiotic functions and can effectively improve the immunity and the anti-stress capability of aquatic animals.
Regulating water quality, reducing pollution and reducing harmful substances: the fermented feed is regularly fed, so that the carbon-nitrogen balance of the water body can be adjusted, and the establishment of the water body bacterial facies is promoted. Meanwhile, the growth and the stability of algae are promoted, the balance of bacteria and algae in the water body is kept, and the stability of the water quality is maintained. The digestion and absorption rate of aquatic animals to the feed is improved by fermenting the wet material, and the pollution of nitrogen in excrement to the substrate is reduced; beneficial bacteria such as Bacillus subtilis, etc. can decompose residual bait, excrement and sludge at the bottom of the pool in a pure biological way through self metabolism, reduce the content of ammonia nitrogen, nitrite and hydrogen sulfide, and increase the dissolved oxygen in water. Can effectively control the water color mutation and reduce the pollution of the pool bottom.
Detailed Description
The technical solution of the present invention is further illustrated by the following specific examples.
Example 1
A wet microbial fermentation material for promoting the growth of aquatic animals comprises the following fermentation raw materials in parts by weight: 48 parts of rice bran, 30 parts of bean pulp, 20 parts of bran, 1.75 parts of honey, 0.075 part of xylanase, 0.02 part of pectinase, 0.02 part of acid protease and 70 parts of water, and uniformly mixing.
The fermentation microorganism comprises 35 parts of saccharomyces cerevisiae, 10 parts of bacillus subtilis, 25 parts of lactobacillus acidophilus, 20 parts of lactobacillus plantarum, 10 parts of clostridium butyricum and 5 parts of honey according to a proportion.
The preparation method of the wet microbial fermentation material comprises the following steps:
1. activating strains: selecting a clean sterilization container, weighing 5 parts of honey according to a proportion, adding a proper amount of activated water to fully dissolve, weighing 35 parts of saccharomyces cerevisiae, 10 parts of bacillus subtilis, 25 parts of lactobacillus acidophilus, 20 parts of lactobacillus plantarum and 10 parts of clostridium butyricum, and placing the materials in the container to be uniformly mixed. Culturing at 30-37 deg.C for 60min to obtain zymogen liquid.
2. Liquid state fermentation: weighing 48 parts of rice bran, 30 parts of soybean meal, 20 parts of bran, 1.75 parts of honey, 0.075 part of xylanase, 0.02 part of pectinase and 0.02 part of acid protease, placing in a fermentation tank, adding 70 parts of tap water, and uniformly mixing. Heating to 40 deg.C for enzymolysis for 40min, heating to 70 deg.C again for sterilization for 30min, and rapidly cooling to 30 deg.C. Uniformly mixing the zymophyte liquid into the fermentation raw materials, fermenting for 72 hours at 30 ℃, observing various indexes, and making detection records.
3. And (3) inspecting and packaging: detecting the pH value, the moisture content and the viable count of the product, and bagging the product after the detection is qualified to prepare a finished product of the wet microbial fermentation material for promoting the growth of aquatic animals.
The addition amount of the fermented wet material is 20kg per ton of feed.
The content of viable bacteria in the wet microbial fermentation material reaches 100 hundred million/g, the water content is kept between 35 and 42 percent, and the proportion of the mixed bacteria is less than or equal to 0.4 part.
Example 2
A wet microbial fermentation material for promoting the growth of aquatic animals is proportionally prepared from rice bran (43 pts. wt.), soybean cake (25 pts. wt.), wheat bran (15 pts. wt.), honey (1.5 pts. wt.), xylanase (0.05 pts. wt.), pectase (0.01 pts. wt.), acidic protease (0.01 pts. wt.) and water (70 pts. wt.) through proportional mixing.
The fermentation microorganism comprises 30 parts of saccharomyces cerevisiae, 9 parts of bacillus subtilis, 20 parts of lactobacillus acidophilus, 15 parts of lactobacillus plantarum, 5 parts of clostridium butyricum and 5 parts of honey according to a proportion.
The preparation method of the wet microbial fermentation material comprises the following steps:
1. activating strains: selecting a clean sterilization container, weighing 5 parts of honey according to a proportion, adding a proper amount of activated water to fully dissolve, weighing 30 parts of saccharomyces cerevisiae, 9 parts of bacillus subtilis, 20 parts of lactobacillus acidophilus, 15 parts of lactobacillus plantarum and 5 parts of clostridium butyricum, and placing the materials in the container to be uniformly mixed. Culturing at 30-37 deg.C for 60min to obtain zymogen liquid.
2. Liquid state fermentation: weighing 43 parts of rice bran, 25 parts of soybean meal, 15 parts of bran, 1.5 parts of honey, 0.05 part of xylanase, 0.01 part of pectinase and 0.01 part of acid protease, placing in a fermentation tank, adding 70 parts of tap water, and uniformly mixing. Heating to 40 deg.C for enzymolysis for 40min, heating to 70 deg.C again for sterilization for 30min, and rapidly cooling to 30 deg.C. Uniformly mixing the zymophyte liquid into the fermentation raw materials, fermenting for 72 hours at 30 ℃, observing various indexes, and making detection records.
3. And (3) inspecting and packaging: detecting the pH value, the moisture content and the viable count of the product, and bagging the product after the detection is qualified to prepare a finished product of the wet microbial fermentation material for promoting the growth of aquatic animals.
The addition amount of the fermented wet material is 20kg per ton of feed.
The content of viable bacteria of the wet microbial fermentation material reaches 100 hundred million/g, the water content is kept between 35 and 42 percent, and the proportion of mixed bacteria is less than or equal to 0.4 part.
Example 3
A wet microbial fermentation material for promoting the growth of aquatic animals is proportionally prepared from rice bran 53, soybean cake 35, wheat bran 25, honey 2, xylanase 0.10, pectase 0.03, acidic proteinase 0.03 and water 70 through proportional mixing.
The fermentation microorganism comprises 40 parts of saccharomyces cerevisiae, 11 parts of bacillus subtilis, 30 parts of lactobacillus acidophilus, 25 parts of lactobacillus plantarum, 15 parts of clostridium butyricum and 5 parts of honey according to a proportion.
The preparation method of the wet microbial fermentation material comprises the following steps:
1. activating strains: selecting a clean sterilization container, weighing 5 parts of honey according to a proportion, adding a proper amount of activated water to fully dissolve, weighing 40 parts of saccharomyces cerevisiae, 11 parts of bacillus subtilis, 30 parts of lactobacillus acidophilus, 25 parts of lactobacillus plantarum and 15 parts of clostridium butyricum, and placing the materials in the container to be uniformly mixed. Culturing at 30-37 deg.C for 60min to obtain zymogen liquid.
2. Liquid state fermentation: weighing 53 parts of rice bran, 35 parts of soybean meal, 25 parts of bran, 2 parts of honey, 0.15 part of xylanase, 0.03 part of pectinase and 0.03 part of acid protease, placing in a fermentation tank, adding 70 parts of tap water, and uniformly mixing. Heating to 40 deg.C for enzymolysis for 40min, heating to 70 deg.C again for sterilization for 30min, and rapidly cooling to 30 deg.C. Uniformly mixing the zymophyte liquid into the fermentation raw materials, fermenting for 72 hours at 30 ℃, observing various indexes, and making detection records.
3. And (3) inspecting and packaging: detecting the pH value, the moisture content and the viable count of the product, and bagging the product after the detection is qualified to prepare a finished product of the wet microbial fermentation material for promoting the growth of aquatic animals.
The addition amount of the fermented wet material is 20kg per ton of feed.
The content of viable bacteria in the wet microbial fermentation material reaches 100 hundred million/g, the water content is kept between 35 and 42 percent, and the proportion of the mixed bacteria is less than or equal to 0.4 part.
To verify the effect of the present invention, the experiment was carried out in a shrimp farm in Hunan, and the fermented wet material prepared in example 1 was an application test example.
The feeding test was carried out in 2019 in a certain shrimp farm in Hunan in 4-5 months. The test shrimp is 6 adjacent shrimp ponds, the control group and the test group have 3 mouths respectively, the shrimp larvae are macrobrachium rosenbergii with the weight and the body length close to each other, and the test period is 30 days. The control group was fed with the basal diet only, and the test groups were added with 20kg/t of the wet microbial fermentation feed prepared in example 1 on the basal day. And recording data such as weight, feeding amount, survival rate and the like during the experiment, and detecting and recording various indexes of water quality.
TABLE 1 growth Performance test results
TABLE 2 Water quality test results
| Control group | 5d | 10d | 15d | 20d | 25d | 30d |
| PH | 7.3 | 7.6 | 7.3 | 6.7 | 7.1 | 7.1 |
| Dissolved oxygen (mg/L) | 5.63 | 5.46 | 5.33 | 5.12 | 4.68 | 4.50 |
| Ammonia nitrogen content (mg/L) | 0.16 | 0.27 | 0.35 | 0.41 | 0.46 | 0.51 |
| Nitrite (mg/L) | 0.05 | 0.05 | 0.06 | 0.08 | 0.09 | 0.14 |
| Hydrogen sulfide (mg/L) | 0.09 | 0.11 | 0.14 | 0.16 | 0.17 | 0.28 |
| Experimental group | 5d | 10d | 15d | 20d | 25d | 30d |
| PH | 7.5 | 7.6 | 7.9 | 7.7 | 7.5 | 7.3 |
| Dissolved oxygen (mg/L) | 6.23 | 6.56 | 6.10 | 5.73 | 5.50 | 5.29 |
| Ammonia nitrogen content (mg/L) | 0.13 | 0.17 | 0.19 | 0.22 | 0.25 | 0.31 |
| Nitrite (mg/L) | 0.04 | 0.03 | 0.05 | 0.05 | 0.05 | 0.07 |
| Hydrogen sulfide (mg/L) | 0.08 | 0.07 | 0.08 | 0.09 | 0.12 | 0.18 |
As shown in the above tables 1 and 2, the body length of the macrobrachium rosenbergii experimental group is increased by 0.53cm in 30 days, the body weight is increased by 0.82g, the survival rate is improved by 0.07%, the feed coefficient is reduced by 0.12, and the growth performance of the macrobrachium rosenbergii can be improved. Meanwhile, the improvement effect on water quality is obvious, the pH value is more stable, harmful substances such as ammonia nitrogen, hydrogen sulfide and nitrite are effectively reduced, the dissolved oxygen is increased, and the aquaculture environment of aquatic animals is improved.
The addition of 20kg/t of wet microbial fermentation feed can obviously improve the growth performance of aquatic animals, reduce the feed coefficient and the death rate, improve the environment of aquaculture water, save the aquaculture cost and improve the aquaculture benefit.
The three embodiments of the present invention have been described in detail, but the description is only for the preferred embodiments of the present invention and should not be construed as limiting the scope of the present invention. All equivalent changes and modifications made within the scope of the present invention shall fall within the scope of the present invention.
Claims (10)
1. A wet microbial fermentation material for promoting the growth of aquatic animals is characterized in that: comprises a fermentation raw material and the fermentation microorganism, wherein the mass of the fermentation microorganism accounts for 0.3-0.5% of the mass of the fermentation raw material.
2. The wet microbial fermentation feed for promoting aquatic animal growth according to claim 1, wherein: the fermentation raw material is prepared by mixing the following components in parts by weight: 43-53 parts of rice bran, 25-35 parts of soybean meal, 15-25 parts of bran, 1.5-2 parts of honey, 0.05-0.10 part of xylanase, 0.01-0.03 part of pectinase, 0.01-0.03 part of acid protease and 70 parts of water.
3. The wet microbial fermentation feed for promoting aquatic animal growth according to claim 2, wherein: the enzyme activity of the fermentation raw material is as follows: the xylanase is more than or equal to 30000u/g, the pectinase is more than or equal to 10000u/g, the acid protease is more than or equal to 60000u/g, and the cellulase is more than or equal to 30000 u/g.
4. The wet microbial fermentation feed for promoting aquatic animal growth according to claim 1, wherein: the fermentation microorganism is prepared by mixing the following components in parts by weight: 30-40 parts of saccharomyces cerevisiae, 9-11 parts of bacillus subtilis, 20-30 parts of lactobacillus acidophilus, 15-25 parts of lactobacillus plantarum, 5-15 parts of clostridium butyricum and 5 parts of honey.
5. The wet microbial fermentation feed for promoting aquatic animal growth according to claim 4, wherein: the viable count of the fermentation microorganism is as follows: the saccharomyces cerevisiae is more than or equal to 1000 hundred million CFU/g, the bacillus subtilis is more than or equal to 1500 hundred million CFU/g, the lactobacillus acidophilus is more than or equal to 1000 hundred million CFU/g, the lactobacillus plantarum is more than or equal to 1000 hundred million CFU/g, and the clostridium butyricum is more than or equal to 1000 hundred million CFU/g.
6. A method for preparing a wet microbial fermentation feed for promoting growth of aquatic animals according to any one of claims 1-5, which comprises: the method comprises the following steps:
step one, strain activation: proportionally placing the fermented microorganisms into a clean sterilization container, adding appropriate activated water, mixing, and culturing in a 30-37 deg.C incubator for 30 min;
step two, liquid state fermentation: placing the fermentation raw material in a liquid fermentation tank, heating to 40 ℃ for enzymolysis for 40min, heating again to 70 ℃ for sterilization for 30min, rapidly cooling to 30 ℃, uniformly mixing the fermentation microorganism and the fermentation raw material in the step one, and performing solid fermentation for 72 hours at 30 ℃;
step three, checking and packaging: detecting the pH value, the moisture content and the viable count of the product, and bagging the product after the detection is qualified to prepare a finished product of the wet microbial fermentation material for promoting the growth of aquatic animals.
7. The method for preparing wet microbial fermentation feed for promoting aquatic animal growth according to claim 6, wherein: the fermentation microorganism in the first step is prepared by mixing the following components in parts by weight: the fermentation microorganism comprises 30-40 parts of saccharomyces cerevisiae, 9-11 parts of bacillus subtilis, 20-30 parts of lactobacillus acidophilus, 15-25 parts of lactobacillus plantarum, 5-15 parts of clostridium butyricum and 5 parts of honey according to a proportion.
8. The method for preparing wet microbial fermentation feed for promoting aquatic animal growth according to claim 6, wherein: the fermentation raw material in the step two is prepared by mixing the following components in parts by weight: 43-53 parts of rice bran, 25-35 parts of soybean meal, 15-25 parts of bran, 1.5-2 parts of honey, 0.05-0.10 part of xylanase, 0.01-0.03 part of pectinase, 0.01-0.03 part of acid protease and 70 parts of water.
9. Use of the wet microbial fermentation feed for promoting aquatic animal growth according to claims 1-6 in the field of feed additives.
10. The use of the wet microbial fermentation feed for promoting aquatic animal growth of claim 9, wherein: the addition amount of the fermented wet material is 20kg per ton of feed.
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