CN110638675A - Terpenoid repellent R2 microemulsion and preparation method thereof - Google Patents

Abstract

The invention discloses a terpene repellent R2 microemulsion and a preparation method thereof, comprising the following components: the repellent R2 is 5-20% by weight, the emulsifier is 5-40% by weight, and the distilled water is 5-40% by weight 40‑90%. In the present invention, the terpenoid repellent R2 is used as the active ingredient, and by means of the emulsification effect of the emulsifier, it is evenly and stably diluted and dispersed in water to form a nano-scale emulsion, so that the terpenoid repellent R2 to be encapsulated can be solubilized and controlled. The terpenoid repellent R2 microemulsion prepared by the invention has good mosquito repellent activity, low product cost, simple preparation method, high economic value and application prospect.

Description

A kind of terpene repellent R2 microemulsion and preparation method thereof

technical field

The invention belongs to the technical field of chemical industry, and in particular relates to a terpenoid repellant R2 microemulsion and a preparation method thereof.

Background technique

Using α-pinene in natural turpentine as raw material, after a series of reactions, hydroxycitronellal 1,2-propanediacetal (R2) can be obtained, the molecular formula is C13H26O3, light yellow liquid, stable under neutral or alkaline conditions . Studies have shown that R2 has good mosquito repellent activity. The repelling time of R2 ethanol solution at 20% mass concentration against Aedes albopictus was 5-6 hours, and the repelling time of R2 ethanol solution with mass concentration of 10% and 20% against Anopheles sinensis were respectively 4.5-5 hours and 5-6 hours, both exceeding the B-level national standard, can be used as repellent for further development.

However, in the actual use process, it is found that R2 itself is a relatively viscous organic compound with a certain greasy feeling. If it is simply formulated into a solution for use, there will be a strong odor, and a few people may still have it when using it. Risk of skin allergy. In addition, the existing formulations still have some defects, such as: not long lasting effect, unsatisfactory weather resistance, poor formulation stability, etc., which hinder the commercialization of repellents.

Repellents can effectively prevent humans from being bitten without killing mosquitoes, which is of great significance for controlling the spread of mosquito-borne diseases and is an important component of comprehensive mosquito control strategies. The development of new repellent formulations is of great significance for giving full play to the efficacy of repellents and overcoming the defects of repellents themselves. The microemulsion formulation can realize the solubilization and controlled release of the inclusion compound, and realize the improvement and prolongation of the drug effect. The feature of this paper is that the preparation of plant-derived repellent compounds into microemulsion solutions enriches the formulations of repellents and has a positive effect on the wide application of repellents.

SUMMARY OF THE INVENTION

The present invention proposes a terpenoid repellent R2 microemulsion and a preparation method thereof to solve the problems raised in the above background technology. The technical scheme of the present invention is realized as follows:

A terpene repellent R2 microemulsion and a preparation method thereof, comprising the following components:

The repellent R2 is 5-20% by weight, the emulsifier is 5-40% by weight, and the distilled water is 40-90% by weight.

In the terpenoid repellent R2 microemulsion of the present invention, the repellent R2 is hydroxycitronellal 1,2-propanediacetal, and the emulsifier is Tween-40, Tween-80 , Polyoxyethylene Castor Oil-30, Polyoxyethylene Castor Oil-35, Polyoxyethylene Castor Oil-40 or Triton X-100.

In the terpene repellent R2 microemulsion of the present invention, the terpene repellent R2 microemulsion is an O/W type microemulsion, and the average particle size is in the range of 1-100 nm.

A preparation method of terpenoid repellent R2 microemulsion, comprising the following steps:

Step 1: Put the terpenoid repellent R2 weighed by 5-20% by weight and the emulsifier weighed by 5-40% by weight into a container, place the container on a constant temperature magnetic stirrer, and at 200 Mix uniformly at a stirring speed of -800r/min, and fully mix to obtain an oil phase composed of terpenoid repellent R2 and emulsifier;

Step 2: The distilled water weighed according to the weight percentage of 40-90% is added to the oil phase of the terpene repellent R2 and emulsifier obtained in step 1, and the container is placed on a constant temperature magnetic stirrer, at 200-800r/min. Mix well at the stirring speed, and fully mix to obtain the terpenoid repellent R2 microemulsion;

Step 3: After storing the terpenoid repellent R2 microemulsion obtained in Step 2 for half a month, centrifuge at a speed of 200-800 r/min for 15-30min to obtain a clear, transparent and unchanged finished terpenoid repellent. Repellent R2 microemulsion.

In the preparation method of the terpene repellent R2 microemulsion of the present invention, the terpene repellent R2 microemulsion is prepared in a temperature environment of 10-30°C.

Implementing this terpene repellent R2 microemulsion of the present invention and its preparation method have the following beneficial effects:

The invention uses the terpenoid repellant R2 as the active ingredient, and makes it evenly and stably diluted and dispersed in water to form a nano-scale emulsion with the help of the action of the emulsifier; Viscosity, ultra-low surface tension, the use of microemulsion to incorporate R2, the terpenoid repellent R2 is evenly distributed in the water phase, and efficiently dissolves, and is made into a water-based microemulsion repellent, which can realize the repellent compound The emulsion can improve the weather resistance of the repellent; it can also realize the slow release of the repellent compound, achieve the purpose of prolonging the repellent effect, and also increase the solubility of the repellent compound in the aqueous solution, making the dosage form more stable; In the case of organic solvents, the problem of low water solubility of terpene repellent R2 is solved, and the stability of terpene repellent R2 products is improved at the same time, which can broaden the application range of terpene repellent R2 and promote its commercialization. has a positive promoting effect.

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below.

Example 1

Take 15g Tween-40/Tween-80/Polyoxyethylene Castor Oil-30/Polyoxyethylene Castor Oil-35/Polyoxyethylene Castor Oil-40/ Triton X-100, and 15g terpenoid repellent Put R2 into the same container, place the container on a constant temperature magnetic stirrer, and mix it evenly at a stirring speed of 800 r/min at a temperature of 25 °C to obtain a terpenoid repellant R2 and an emulsifier. Then add 70g of deionized water to the oil phase of the obtained terpenoid repellent R2 and emulsifier, place the container on a constant temperature magnetic stirrer, under the temperature environment of 25 ° C, at 200 r/min Mix well under the stirring speed of 100%, fully mix to obtain terpene repellent R2 microemulsion, the microemulsion is O/W type microemulsion, the average particle size is 48.50nm, the R2 inclusion rate is 15%, and it is clear and transparent; The obtained terpene repellent R2 microemulsion was stored for half a month, and then centrifuged at a speed of 600 r/min for 20 minutes to obtain a clear, transparent and unchanged finished terpene repellent R2 microemulsion. The efficacy of Aedes mosquitoes reaches or exceeds the B-level standard stipulated in the national standard GB/T 13917.9-2009: the effective protection time is greater than or equal to 4h.

Example 2

Take 18g Tween-40/Tween-80/Polyoxyethylene Castor Oil-30/Polyoxyethylene Castor Oil-35/Polyoxyethylene Castor Oil-40/ Triton X-100, and 12g terpenoid repellent Put R2 into the same container, place the container on a constant-temperature magnetic stirrer, mix evenly at a stirring rate of 300 r/min at a temperature of 20 °C, and thoroughly mix to obtain terpenoid repellent R2, emulsifier Then add 70g of deionized water into the oil phase of the obtained terpenoid repellent R2 and emulsifier, place the container on a constant temperature magnetic stirrer, under the temperature environment of 20 ° C, at 300 r/min Mix well under the stirring speed of 100%, fully mix to obtain the terpenoid repellant R2 microemulsion, the microemulsion is an O/W type microemulsion, the average particle size is 26.05nm, the R2 inclusion rate is 15%, and it is clear and transparent; the obtained The terpenoid repellent R2 microemulsion was stored for half a month, and then centrifuged at a speed of 600r/min for 20min in a centrifuge to obtain a clear, transparent and unchanged finished terpene repellent R2 microemulsion. The efficacy of mosquitoes reaches or exceeds the B-level standard specified in the national standard GB/T 13917.9-2009: the effective protection time is greater than or equal to 4h.

Example 3

Take 40g Tween-40/Tween-80/Polyoxyethylene Castor Oil-30/Polyoxyethylene Castor Oil-35/Polyoxyethylene Castor Oil-40/ Triton X-100, and 20g terpenoid repellent Put R2 into the same container, place the container on a constant-temperature magnetic stirrer, mix evenly at a stirring rate of 200 r/min at a temperature of 10 °C, and thoroughly mix to obtain terpenoid repellent R2, emulsifier Then add 40g of deionization into the oil phase of the obtained terpene repellent R2 and emulsifier, place the container on a constant temperature magnetic stirrer, and at a temperature of 10 °C, at a temperature of 800r/min. The mixture is uniformly mixed at a stirring speed, and the terpenoid repellent R2 microemulsion is obtained by fully mixing. The terpenoid repellent R2 microemulsion was stored for half a month, and then centrifuged at 200 r/min for 30 minutes to obtain a clear, transparent and unchanged finished terpene repellent R2 microemulsion. The efficacy of mosquitoes reaches or exceeds the B-level standard specified in the national standard GB/T 13917.9-2009: the effective protection time is greater than or equal to 4h.

Example 4

Take 5g of Tween-40/Tween-80/Polyoxyethylene Castor Oil-30/Polyoxyethylene Castor Oil-35/Polyoxyethylene Castor Oil-40/Triton X-100, and 5g of terpenoid repellent Put R2 into the same container, place the container on a constant-temperature magnetic stirrer, mix evenly at a stirring rate of 800 r/min at a temperature of 30 °C, and thoroughly mix to obtain terpenoid repellent R2 and emulsifier. Then add 90g of deionized water into the oil phase of the obtained terpenoid repellent R2 and emulsifier, place the container on a constant temperature magnetic stirrer, under the temperature environment of 30 ℃, at 200r/min Mix well under the stirring speed of 100%, fully mix to obtain the terpenoid repellent R2 microemulsion, the microemulsion is O/W type microemulsion, the average particle size is 1-100nm, the R2 inclusion rate is 15%, and the microemulsion is clear and transparent; The obtained terpene repellent R2 microemulsion was stored for half a month, and then centrifuged at a speed of 800 r/min for 15 min with a centrifuge to obtain a clear, transparent and unchanged finished terpene repellent R2 microemulsion. The efficacy of Aedes mosquitoes reaches or exceeds the B-level standard specified in the national standard GB/T 13917.9-2009: the effective protection time is greater than or equal to 4h.

The terpene repellent R2 microemulsion prepared by the invention takes the terpene repellent R2 as an active ingredient, and is evenly and stably diluted and dispersed in water to form a nano-scale emulsion by means of the emulsification of the emulsifier. The microemulsion has good repellency Mosquito activity, low product cost, simple preparation method, high economic value and application prospect.

Examples are only examples, and are not intended to limit the present invention. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included within the protection scope of the present invention.

Claims (5)

1. a terpene repellent R2 microemulsion, is characterized in that, comprises the following components: The repellent R2 is 5-20% by weight, the emulsifier is 5-40% by weight, and the distilled water is 40-90% by weight. 2. terpene repellent R2 microemulsion according to claim 1, is characterized in that, described repellent R2 is hydroxycitronellal 1,2-propanediacetal, and described emulsifier is sputum Win-40, Tween-80, Polyoxyethylene Castor Oil-30, Polyoxyethylene Castor Oil-35, Polyoxyethylene Castor Oil-40, or Triton X-100. 3 . The terpene repellent R2 microemulsion according to claim 1 , wherein the terpene repellent R2 microemulsion is an O/W type microemulsion, and the average particle size range is 1-100 nm. 4 . 4. a terpenoid repellent R2 microemulsion preparation method, is characterized in that, comprises the following steps: Step 1: put the terpenoid repellent R2 weighed in 5-20% by weight and the emulsifier weighed in 5-40% by weight into a container, the container is placed on a constant temperature magnetic stirrer, at 200 Mix well at a stirring speed of -800 r/min, and fully mix to obtain an oil phase composed of terpenoid repellent R2 and emulsifier; Step 2: The distilled water weighed according to weight percentage 40-90% is added to the terpene repellent R2 and the oil phase of the emulsifier obtained in step 1, and the container is placed on a constant temperature magnetic stirrer, and at 200-800 r/ Mix uniformly at the stirring speed of min, and fully mix to obtain terpenoid repellent R2 microemulsion; Step 3: After storing the terpene repellent R2 microemulsion obtained in step 2 for half a month, centrifuge at a speed of 200-800r/min for 15-30min with a centrifuge to obtain a clear, transparent and unchanged finished terpenoid repellent. Agent R2 microemulsion. 5 . The method for preparing a terpene repellent R2 microemulsion according to claim 4 , wherein the terpene repellent R2 microemulsion is prepared in a temperature environment of 10-30° C. 6 .