CN110420154A - A kind of Anti-hair loss traditional Chinese medicine composition and the preparation method and application thereof - Google Patents

Abstract

The invention discloses a traditional Chinese medicine composition for preventing hair loss, a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following components in parts by weight: 5-50 parts of shegan; 5-35 parts of hibiscus bark; 5-35 parts of three needles; 5-35 parts of Xu Changqing; and 5-35 parts of licorice. The traditional Chinese medicine composition of the present invention is guided by the traditional Chinese medicine prescription theory of monarchs, ministers and assistants. Various components are matched according to specific ratios to achieve synergistic effects. To prevent hair loss, use the blood-activating effect of the traditional Chinese medicine composition to revitalize the hair root to promote hair growth, and use the antibacterial and anti-oxidant effects of the traditional Chinese medicine composition to achieve the effect of anti-dandruff, so as to achieve the comprehensive treatment goal of hair loss.

Description

A kind of anti-hair loss traditional Chinese medicine composition and its preparation method and application

technical field

The invention belongs to the field of cosmetics, and in particular relates to a traditional Chinese medicine composition for preventing hair loss, a preparation method and application thereof.

Background technique

With the continuous improvement of people's living standards and quality of life, consumers have higher and higher requirements for beauty and skin care products necessary for daily life, and pay more and more attention to the influence of the concept of "returning to nature" and tending to plant skin care.

In recent years, due to factors such as people's accelerated pace of life, staying up late, irregular diet, dyed hair, and environmental pollution, scalp health problems have become increasingly prominent. According to data from the Scalp Health Research Center of the China Health Education Association, more than 60% of adults in my country have different degrees of health problems on their scalps. One of the most serious scalp problems is hair loss.

Alopecia is mainly divided into two types: physiological alopecia and seborrheic alopecia. Physiological hair loss is often due to the atrophy and thinning of the muscle layer of the scalp tissue, which makes the blood flow of the microfilament vascular network decrease and slow down, which will become more obvious with age. Seborrheic alopecia is a symptom of hyperplasia and hypertrophy due to the influence of androgen in the sebaceous gland receptors, which wraps the hair root, and the hair root cannot provide enough nutrition to the hair shaft, and the hair shaft will suffer from nutritional cut-off and hair loss; Scalp oil excretion increases, infection causes scalp acne and folliculitis, damages hair follicles and causes permanent hair loss. Therefore, the scientific anti-hair loss method should carry out comprehensive treatment from the aspects of oil control, inhibiting the growth of Malassezia, clearing inflammation, and promoting blood circulation.

At present, the anti-hair loss drugs are still mainly chemical substances, such as finasteride, apretide, eristide, dutasteride, ketoconazole and other western medicine ingredients, but the western medicine ingredients are very irritating and have great side effects, so they cannot be used. Used in cosmetics. In order to overcome the defects of chemical substances, the present invention uses a traditional Chinese medicine composition with low irritation to human body and low toxicity as the anti-hair loss active substance to achieve the purpose of anti-hair loss.

Contents of the invention

In order to solve the shortcomings and deficiencies of the prior art, the primary purpose of the present invention is to provide a Chinese medicine composition for preventing hair loss. Based on the TCM theory of monarchs, ministers, assistants and envoys, the invention provides an anti-hair loss traditional Chinese medicine composition which has the functions of controlling oil, inhibiting the growth of Malassezia bacteria, clearing inflammation and promoting blood circulation.

Another object of the present invention is to provide a preparation method of the above-mentioned anti-hair loss traditional Chinese medicine composition. A micro-jet wall-breaking extraction method is adopted, that is, the Chinese medicine mixture is subjected to expansion and explosion extraction, centrifugal filtration, membrane concentration-macroporous resin adsorption-membrane concentration, and the Chinese medicine composition is obtained.

Another object of the present invention is to provide the application of the above-mentioned traditional Chinese medicine composition for preventing hair loss in cosmetics.

Another object of the present invention is to provide a hair lotion containing the above-mentioned anti-hair loss traditional Chinese medicine composition.

The object of the invention is achieved through the following technical solutions:

A traditional Chinese medicine composition for preventing hair loss, which is made of the following components in parts by weight: 5-50 parts of shegan; 5-35 parts of hibiscus bark; 5-35 parts of three needles; share.

Preferably, the anti-hair loss traditional Chinese medicine composition is made of the following components by weight: Shegan 10-45 parts; Hibiscus bark 7-30 parts; Three needles 7-30 parts; Xu Changqing 5-30 parts; 5-30 parts of licorice.

Further preferably, the anti-hair loss traditional Chinese medicine composition is made of the following components by weight: Shegan 15-40 parts; Hibiscus bark 10-25 parts; Three needles 10-25 parts; Xu Changqing 10-30 parts ; 10-25 parts of licorice.

Further preferably, the anti-hair loss traditional Chinese medicine composition is made of the following components by weight: Shegan 25-30 parts; Hibiscus bark 15-20 parts; Three needles 15-20 parts; Xu Changqing 18-25 parts ; licorice 15 to 22 parts.

The preparation method of above-mentioned a kind of anti-hair loss Chinese medicine composition, comprises the steps:

①Ingredients: Remove impurities from Shegan, Tujinpi, Sanjiazhen, Xu Changqing and Licorice respectively, wash, dry, crush, pass through a 40-100 mesh sieve, and then mix to obtain a Chinese medicine mixture;

②Extraction: Mix the Chinese medicine mixture and 1,3-butanediol evenly, soak, then extract the mixture of Chinese medicine mixture and 1,3-butanediol through a micro-fluidic extractor, centrifuge, filter, discard the precipitate, and collect filtrate;

③ Concentration: Take the filtrate and concentrate it with a membrane to obtain the traditional Chinese medicine composition.

Preferably, the impurity removal, cleaning, drying and pulverization in step ① all adopt conventional methods in the art.

Preferably, the traditional Chinese medicine mixture and 1,3-butanediol in step ② are in a weight ratio of 1:1 to 15, and the soaking time is 1 to 5 hours; the centrifugation speed is 1000 to 3000 rpm, and the centrifugation time 0.5 to 10 minutes.

Preferably, the microfluidic extractor described in step ② is the microfluidic extractor described in the patent CN107537180A.

Preferably, the filtrate in step ③ is concentrated with a membrane to 1-3 times the weight of the traditional Chinese medicine mixture in step ②.

Preferably, the membrane concentration described in step ③ adopts conventional methods in the art.

Preferably, steps ①～③ are all carried out at room temperature.

Application of the above-mentioned anti-hair loss traditional Chinese medicine composition in cosmetics.

Preferably, the application is: using the above-mentioned anti-hair loss traditional Chinese medicine composition for preparing cosmetics, wherein the anti-hair loss traditional Chinese medicine composition accounts for 0.1-60% by weight of the cosmetic.

Preferably, the anti-hair loss traditional Chinese medicine composition accounts for 1-30% by weight of the cosmetic.

An anti-hair loss cosmetic, which contains the above-mentioned anti-hair loss traditional Chinese medicine composition, and the weight content of the anti-hair loss traditional Chinese medicine composition in the anti-hair loss cosmetic is 0.1-60%.

Preferably, the weight content of the anti-hair loss traditional Chinese medicine composition in the anti-hair loss cosmetic is 1-30%.

Preferably, the anti-hair loss cosmetics are hair care cosmetics; the hair care cosmetics are one of lotions, emulsions, creams, sprays, essences and soaps.

A hair care and anti-loss nutritional water, which consists of the following components in parts by mass:

The hydrogenated castor oil CO40 is polyoxyethylene 40 hydrogenated castor oil.

An anti-shedding shampoo for scalp care, which consists of the following components in parts by mass:

The polyquaternium-5 is N,N,N-trimethyl-2-[(2-methyl-1-oxygen-2-propenyl)oxy]ethylammonium methanesulfonate and 2-acrylamide of polymers.

A scalp care anti-hair loss conditioner, consisting of the following components in terms of mass fractions:

The mass ratio of cetyl alcohol to stearyl alcohol in the cetostearyl alcohol is 3:7.

The polyquaternium-37 is a homopolymer of N,N,N-trimethyl-2-[(2-methyl-1-oxo-2-propenyl)oxy]ethylammonium methanesulfonate.

The traditional Chinese medicine composition for preventing hair loss described in the present invention is guided by the theory of TCM prescriptions of Jun Chen Zuo Shi, and is obtained by selecting She Gan, Tu Hibiscus Pi, Three Needles, Xu Changqing and Licorice according to a certain proportion. In the composition of the present invention, Shegan is the monarch drug, which has the effects of clearing away heat and detoxification, dispelling stagnation, reducing inflammation, reducing swelling and relieving pain, and achieves the purpose of oil control, removing scalp acne and treating folliculitis; All three needles have the effects of clearing away heat and promoting dampness, antibacterial, and dispersing blood stasis, helping to achieve the purpose of oil control, clearing scalp acne, and treating folliculitis; Xu Changqing is an adjuvant drug, which has the effects of dispelling wind, relieving itching, dredging menstrual flow and activating blood circulation, and achieving the goal of revitalizing hair roots Glycyrrhiza licorice is used as medicine, has the effects of nourishing qi and invigorating the middle, relieving spasm and relieving pain, purging fire and detoxification, and harmonizing various medicines.

Compared with the prior art, the present invention has the following advantages and beneficial effects:

The components in the anti-hair loss Chinese medicine composition of the present invention are matched according to a specific ratio to achieve a synergistic effect. Utilizing the bactericidal and oil-controlling effects of the Chinese medicine composition to remove scalp acne and treat folliculitis so as to prevent hair loss, use the combination of Chinese medicine The anti-dandruff effect is achieved by using the anti-bacterial and anti-oxidative effects of the traditional Chinese medicine composition, so as to achieve the goal of comprehensive treatment of hair loss.

Description of drawings

Fig. 1 is a color comparison diagram of the traditional Chinese medicine composition obtained in Example 1 and the traditional Chinese medicinal composition obtained in Comparative Examples 6-9.

Detailed ways

The present invention will be further described in detail below with reference to the examples and drawings, but the implementation of the present invention is not limited thereto.

The test methods for which specific experimental conditions are not indicated in the examples and comparative examples of the present application are all operated according to conventional experimental conditions and methods in this field.

Embodiment 1 A kind of anti-hair loss Chinese medicine composition (I)

A traditional Chinese medicine composition for preventing hair loss, which is prepared from the following components in parts by weight: 30 parts of shegan; 15 parts of hibiscus bark; 15 parts of three needles; 20 parts of Xu Changqing; 20 parts of licorice.

Embodiment 2 A kind of anti-hair loss Chinese medicine composition (Ⅱ)

A traditional Chinese medicine composition for preventing hair loss, which is prepared from the following components in parts by weight: 25 parts of shegan; 20 parts of hibiscus bark; 15 parts of three needles; 25 parts of Xu Changqing; 15 parts of licorice.

Embodiment 3 A kind of anti-hair loss Chinese medicine composition (Ⅲ)

A traditional Chinese medicine composition for preventing hair loss, which is prepared from the following components in parts by weight: 25 parts of shegan; 15 parts of hibiscus bark; 20 parts of three needles; 18 parts of Xu Changqing; 22 parts of licorice.

Embodiment 4 A kind of anti-hair loss Chinese medicine composition (Ⅳ)

A traditional Chinese medicine composition for preventing hair loss, which is prepared from the following components in parts by weight: 30 parts of shegan; 15 parts of hibiscus bark; 18 parts of three needles; 20 parts of Xu Changqing; 17 parts of licorice.

Examples 1-4 Preparation method: Under room temperature conditions, remove impurities, wash, dry, and pulverize the raw materials of Chinese medicinal materials respectively, pass through a 60-mesh sieve, and mix evenly to obtain a Chinese medicine mixture, add it to a conventional extraction tank, and add the weight of the Chinese medicine mixture 10 times of 1,3-butanediol was soaked for 3 hours, and then the mixture of traditional Chinese medicine mixture and 1,3-butanediol was extracted by a micro-fluidic extractor (see patent CN107537180A) (continuous micro-jet broken wall at room temperature extraction), then centrifuged at 2000 rpm for 2min and filtered, and the filtrate was concentrated to 2 times the weight of the Chinese medicine mixture with a membrane, which was the Chinese medicine composition provided by the invention.

Comparative example 1-5 single Chinese medicine extract

The ingredients are shown in Table 1.

Table 1

Element Comparative example 1 Comparative example 2 Comparative example 3 Comparative example 4 Comparative example 5 Shegan 100 copies 0 copies 0 copies 0 copies 0 copies hibiscus bark 0 copies 100 copies 0 copies 0 copies 0 copies three needles 0 copies 0 copies 100 copies 0 copies 0 copies Xu Changqing 0 copies 0 copies 0 copies 100 copies 0 copies licorice 0 copies 0 copies 0 copies 0 copies 100 copies

According to the preparation methods described in Examples 1 to 4, the extracts of Shegan, Tujinpi, Sangezhen, Xu Changqing and Licorice corresponding to Comparative Examples 1 to 5 were sequentially prepared to obtain single herbal extracts.

Comparative example 6-9

In Comparative Example 6, the 1,3-butanediol in the preparation method of Example 1 was changed to deionized water, and the rest of the conditions were completely consistent with those of Example 1 to obtain a Chinese medicine extract, which was a Chinese medicine composition.

In Comparative Example 7, the 1,3-butanediol in the preparation method of Example 1 was changed to absolute ethanol, and the rest of the conditions were completely consistent with those of Example 1 to obtain a Chinese medicine extract, which was a Chinese medicine composition.

Comparative example 8 is that the traditional Chinese medicine extract obtained in comparative example 6 is adsorbed with AB-8 macroporous resin, and then eluted successively with ethanol with a volume fraction of 20%, 50%, and 95%, and the eluate is collected, and the eluate Concentrating by membrane to the same weight as the traditional Chinese medicine extract before adsorption by the macroporous resin to obtain a concentrated solution, adding butanediol with the same weight as the concentrated solution to dissolve, filtering to obtain the traditional Chinese medicine extract, which is the traditional Chinese medicine composition.

Comparative Example 9 is to absorb the extract obtained in Comparative Example 7 with AB-8 macroporous resin, then elute sequentially with ethanol with a volume fraction of 20%, 50%, and 95%, collect the eluate, and separate the eluate from the membrane Concentrate to the same weight as the traditional Chinese medicine extract before adsorption by the macroporous resin to obtain a concentrated solution, then add butanediol with the same weight as the concentrated solution to dissolve, filter to obtain the traditional Chinese medicine extract, which is the Chinese medicine composition.

Test Example 1 Zone of Inhibition Test

The test method is the Oxford cup method (also called the tube-and-dish method). This method is a common method for determining the potency of antibiotics at home and abroad, and it is also a method stipulated by multinational pharmacopoeias. Through the inhibition zone test, it is judged that the sample to be tested is resistant to Malassezia furfur (Malassezia furfur (Pityrosporum), Latin Malassezia furfur (Pityrosporum orbitale), purchase unit: Guangdong Microbial Culture Collection Center, GIM2.181 ) inhibition.

Test samples: the products prepared in Examples 1-4 and Comparative Examples 1-5 were diluted to 5 times with olive oil as samples to be tested.

With aseptic operation, pour the culture medium that has been sterilized at 0.105MPa pressure and 121°C for 30min into sterile petri dishes with a diameter of 9cm, so that each petri dish has 15mL of culture medium. After cooling, add 0.2mL Mala Chromobacterium suspension (10 ⁵ ~ 10 ⁶ cfu/g), spread evenly on the surface of the culture medium with a coating stick, place the Oxford cup vertically on the surface of the corresponding culture medium with tweezers, press gently to make it compatible with the culture medium There are no gaps in the substrate contact, and each petri dish can hold 3 small tubes, and drop 0.1mL of various samples to be tested into each small tube, and mark it well to prevent it from overflowing. Malassezia: Put the above-mentioned experimental petri dish in a 37°C incubator for anaerobic culture for 2 to 5 days, and observe the results.

During cultivation, on the one hand, the test bacteria begin to grow, and on the other hand, the sample to be tested spreads around from the Oxford cup as the origin, forming a circular area where the colony cannot grow, which is the "inhibition zone". The larger the antibacterial zone, the better the effect of acne removal.

The results of the drug susceptibility test are based on the diameter of the inhibition zone to determine the sensitivity level, and the judgment standard refers to the following table 2.

Table 2 Judgment Criteria for Drug Sensitivity Test

Antibacterial zone diameter (mm) sensitivity over 20 extremely sensitive 15～20 high sensitivity 10～14 Zhongmin Below 10 Hypoallergenic 0 insensitive

After culturing for 5 days, the test results are shown in Table 3.

Table 3 Bacterial inhibition zone test test results

The results show that Examples 1 to 4 in the present invention have a significant effect on inhibiting Malassezia, indicating that the Chinese medicine composition has significant antibacterial and anti-inflammatory effects; and the effects of the single Chinese medicines described in Comparative Examples 1 to 5 are much better , indicating that the traditional Chinese medicine has a synergistic effect on the inhibition of Malassezia after scientific formulation.

Test Example 2 Inhibition of Steroidal 5α-Reductase Activity Effect Evaluation Test

Scientific research has found that androgen, especially the special androgen of dihydrotestosterone (abbreviated as DHT), is too hyperactive to be the main factor leading to seborrheic alopecia. The DHT synthesis process requires the participation of steroidal 5α-reductase, so the synthesis of DHT can be inhibited by inhibiting the activity of steroidal 5α-reductase, so as to achieve the purpose of preventing hair loss.

Test samples: blank test group (steroidal 5α-reductase, dependent reducing coenzyme (NADPH), Tris buffer solution); blank control group (testosterone ethanol solution, steroidal 5α-reductase, dependent reducing coenzyme (NADPH), Tris buffer solution); Example 1 sample group (embodiment 1 Chinese medicine composition aqueous solution, testosterone ethanol solution, steroidal 5α-reductase, dependent reducing coenzyme (NADPH), Tris buffer solution); Comparative example 1～5 sample groups (comparative example 1～5 Chinese medicine extract aqueous solution, testosterone ethanol solution, steroidal 5α-reductase, dependent reducing coenzyme (NADPH), Tris buffer solution); Wherein the concentration of testosterone ethanol solution is 0.4 μ mol/L, embodiment 1 The concentration of the aqueous solution of the Chinese medicine composition is 0.5g/L, and the concentration of the aqueous solution of the Chinese medicine extract of Comparative Examples 1 to 5 is 0.5g/L; It was prepared by the method described in the literature (Sun Zuyue, Zheng Weijun, Wang Xiaolin. A new assay method for steroidal 5α-reductase activity [J], Chinese Journal of Endocrinology and Metabolism, 1998, 14(3): 206-207).

According to the literature (Sun Zuyue, Zheng Weijun, Wang Xiaolin. A new assay method for steroidal 5α-reductase activity [J], Chinese Journal of Endocrinology and Metabolism, 1998, 14(3): 206-207) to carry out the test: the above groups of reaction systems Continuously incubate at 37°C, measure the time-varying curve of the OD value of the characteristic absorption wavelength of NADPH continuously at 340nm with an ultraviolet grating spectrophotometer, and calculate the concentration drop rate of NADPH as the enzymatic reaction substrate within 10 minutes according to the NADPH standard curve , subtract the rate of decline of the blank test group, which is the enzyme activity. The measurement results are shown in Table 4.

Table 4 Steroidal 5α-reductase activity

The results in Table 4 show that the Chinese medicine composition of Example 1 of the present invention has a significant inhibitory effect on steroidal 5α-reductase activity, and its inhibitory effect is significantly better than that of the single Chinese medicine described in Comparative Examples 1 to 5, indicating that The traditional Chinese medicine has a synergistic effect on the inhibition of steroidal 5α-reductase activity after scientific formulation.

Test example 3 free radical scavenging test

1. Sample preparation

The products prepared in Examples 1-4 were formulated into sample ethanol solutions with a mass concentration of 1%, and the following experiments were carried out.

2. Evaluation of superoxide anion free radical scavenging ability

Take 4.5 mL of 0.05 mol/L Tris-HCl buffer solution with pH=8.2, and preheat it in a water bath at 25°C for 20 min. Then add 1mL sample ethanol solution and 0.4mL 25mmol/L pyrogallol solution, mix well, react in a water bath at 25°C for 5min, and add 1.0mL 8mol/L HCl to terminate the reaction. Using Tris-HCl buffer solution (0.05mol/L pH=8.2) as a reference, the absorbance value was measured at 299nm. For the blank control, use 1mL sample ethanol solution as a solvent to replace the sample, and other conditions remain unchanged, carry out the above operations, and measure the absorbance value of the blank control.

Superoxide anion radical scavenging rate (%)=[1-(A2-A1)/A1]×100%

In the formula, A1 is the absorbance value of the blank control; A2 is the absorbance value of the sample.

3. Evaluation of the ability to scavenge hydroxyl radicals

Add 3mL of 2mmol/L FeSO ₄ solution and 3mL of 1mmol/L H ₂ O ₂ solution to a 25mL colorimetric tube successively, shake well, then add 3mL of 6mmol/L salicylic acid solution, shake well, heat in a water bath at 37°C for 15min, then take out , measure its absorbance A3; then add 1mL sample ethanol solution, shake well, continue heating in a water bath for 15min, take it out and measure its absorbance A4. The blank control group was no salicylic acid solution, and the other conditions remained unchanged. The above operations were carried out, and the absorbance A5 was measured. The following formula is the scavenging rate of the liquid to be tested to hydroxyl radicals (OH):

Hydroxyl radical scavenging rate (%)=[A3－A4－(A3－A5)]/A3×100%

In the formula, A3 is the absorbance value of the reaction system before no sample is added; A4 is the absorbance value of the system after the sample removes OH; A5 is the absorbance value of the system after the blank control removes OH.

4. Comparative test of samples against free radicals

Table 5 Functional evaluation results of free radical scavenging rate

Experiments show that the traditional Chinese medicine compositions of Examples 1 to 4 of the present invention have a good scavenging effect on superoxide anion and hydroxyl radical.

Test example 4 skin irritation test

Experimental materials: the samples of Example 1 and Comparative Examples 1-5 were dissolved in butanediol to prepare a solution with a mass fraction of 20% as a test sample.

Subjects: 30 volunteers in total, including 20 females and 10 males, aged between 20 and 40 years old.

Test method: Take 0.03mL test sample with a syringe and place it in the medicine chamber of the spot test tape, and immediately stick the spot test tape with the test sample on the normal skin of the left forearm longitudinally from the bottom, and at the same time lightly press the medicine chamber one by one to remove air, and distribute the test substance evenly. Mark the test site for easy observation. The skin was applied every 24 hours, and half an hour after the patch was removed, the inspection was carried out according to the terms of the CTFA guideline (1981). A total of 6 patch tests were performed (applied to the same position each time), and an additional examination was performed on the 6th day after the end of the patch to observe delayed reactions. Data were compared using the results of the last inspection. The identification is carried out according to the following table 6 as the standard:

Table 6 Skin Irritation Identification Criteria

grade symbol Identification standard 0 - Negative reaction: no irritation, no erythema 1 ± Suspicious reaction: mild erythema 2 + Weak positive reaction: erythema 3 ++ Strong positive reaction: erythema, papules, vesicles 4 +++ Very strong positive reaction: severe edema, bullae

Test results: as shown in Table 7, where 0 represents a grade of 0, that is, no irritation and no erythema.

Table 7 Skin irritation test results

Skin times Example 1 Comparative example 1 Comparative example 2 Comparative example 3 Comparative example 4 Comparative example 5 1 0 1 time± 1 time± 1 time± 1 time± 1 time± 2 0 0 0 0 1 time± 0 3 0 0 0 1 time± 0 0 4 0 1 time± 0 0 0 0 5 0 0 1 time+ 0 0 0 6 0 0 0 0 0 0 total 0 2 times ± 1 time ±, 1 time + 2 times ± 2 times ± 1 time±

The results in Table 7 show that no irritation was found in volunteers in the Chinese medicine composition sample of Example 1 of the present invention, and the irritation was much lower than that of the single Chinese medicine samples in Comparative Examples 1-5. It can be seen from this that the scientific combination of traditional Chinese medicine is milder than that used alone under the condition of consistent dosage.

Test Example 4 Oil Control Effect Evaluation Test

4.1 Sample preparation and instruments

Experimental materials: the samples of Example 1 and Comparative Examples 1-5 were dissolved in water to prepare a solution with a mass fraction of 20% as a test sample.

The test instrument is a Sebumeter test probe for skin oil content manufactured by German Courage+Khazaka Company (Germany CK Company).

4.2 Experimental principle

The Skin Oil Clearance Test allows for accurate quantitative numerical comparisons of the efficacy of oil control products.

4.3 Test method of forehead grease removal rate

Select 30 volunteers with oily skin, aged between 20 and 35, half male and half male. Volunteers were not allowed to use any oil control products, such as cosmetics or external medicines or internal health care products, 30 days before the start of the test. Before the test, sit in a constant temperature and humidity room with a temperature of 22±2°C and a humidity of 50±5% for 30 minutes, select the left and right foreheads of the volunteers according to the random table, and take the intersection point of the midline between the eyebrows and the parallel line 1cm away from the upper eyebrow edge as Test the midpoint, then test one position side by side from the left to the right at the midpoint of the test, take the average of the three values as the fat data of the forehead 0h, and then ask the subjects to use the test sample stickers on one side of the forehead according to the random table Wash after 15 minutes of application, and perform grease test at each time point of 30min, 2h, and 4h after washing. The blank control is the other side, which is only applied and washed with deionized water.

4.4 Test results

Table 8 forehead fat reduction (%)

The data in Table 8 shows that Example 1 has an obvious effect of inhibiting skin oil secretion, and comparative examples 1 to 5 have a significant inhibitory effect on skin oil secretion, indicating that the oil control effect of traditional Chinese medicine has a synergistic effect after scientific formulation.

Test Example 5 Color and conductivity determination test

The conductivity of natural substance extracts is an important factor affecting the stability of cosmetics, and the color is an important factor affecting the appearance of cosmetics. Therefore, the conductivity and color of natural substance extracts used in cosmetics should be controlled.

The conductivity of the samples of Example 1 and Comparative Examples 6-9 was measured with a conductivity meter, and the results are shown in Table 9.

Table 9

It can be seen from Table 9 that the conductivity of Example 1 is much smaller than that of Comparative Examples 6 to 7, and is equivalent to that of Comparative Examples 8 to 9, indicating that using 1,3-butanediol as a solvent can obtain lower conductivity than water or ethanol The conductivity of the extract is even slightly better than that of water or ethanol as solvent and further purified extract. The conductivity of 1,3-butanediol is 1.32 μS/cm, and that of deionized water is 2.78 μS/cm.

Fig. 1 is the color comparison diagram of the Chinese medicine composition obtained in Example 1 and the Chinese medicine composition obtained in Comparative Examples 6 to 9. As can be seen from the figure, the color of Example 1 is much lighter than that of Comparative Examples 6 to 7, and it is much lighter than that of Comparative Examples 6 to 7. 8 to 9 are equivalent.

It shows that the color and conductivity of the extract obtained by direct extraction with 1,3-butanediol as a solvent can meet the requirements of natural substance extracts for cosmetics, and cumbersome purification steps can be omitted.

Application example 1 Hair care and anti-loss nutrient water

A kind of anti-hair loss nutrient water containing the anti-hair loss Chinese medicine composition described in embodiment 1, the specific formula is as shown in table 10:

Table 10

Among them, silicone oil DC949 was purchased from Dow Corning (Shanghai) Co., Ltd., and hydrogenated castor oil CO40 was purchased from BASF China Co., Ltd.

Preparation:

(1) Heat the material of component A to 85°C, keep it warm for 30 minutes, stir and dissolve completely;

(2) Heat the material of component B to 85°C, stir and dissolve completely;

(3) Add component B to component A, homogenize for 5 minutes, stir, cool down to 50°C, add components C and D, stir evenly, cool down to 38°C, and you can get hair care and anti-hair loss nutritional water.

Application Comparative Example 1 Ordinary hair care anti-dehydration nutrient water

Except not containing the traditional Chinese medicine composition of the present invention, other conditions are consistent with Application Example 1.

Application example 2 Scalp care anti-shedding shampoo

A scalp care and anti-shedding shampoo containing the anti-hair loss Chinese medicine composition described in Example 2, the specific formula is as shown in Table 11:

Table 11

Application Example 3 Scalp Care Anti-Hairfall Conditioner

A kind of scalp care anti-hair loss conditioner containing the anti-hair loss Chinese medicine composition described in embodiment 3, the specific formula is as shown in table 12:

Table 12

Substance name Mass fraction/% Deionized water 73.7 cetostearyl alcohol 5.00 Polyquaternium-37 1.50 monoglyceride 2.00 Methylparaben 0.10 Propylparaben 0.05 Allantoin 0.20 Hydroxyethyl cellulose 1.00 Chinese medicine composition described in embodiment 3 10.0 Oat beta-glucan 2.00 Amodimethicone 2.00 Sericin 0.80 Sodium PCA 0.20 essence 0.80 lactic acid 0.65

Among them, the mass ratio of cetyl alcohol to stearyl alcohol in cetostearyl alcohol is 3:7.

Scalp Effect Evaluation

Evaluation samples: the products prepared in application example 1 (hair care and anti-loss nutrient water) and application comparative example 1 (common hair loss and anti-loss nutrient water).

Evaluation method: Select 40 subjects with seborrheic alopecia, all of them are male, aged 30-45 years old. The method of use is to spray the sample on the scalp after each shampoo and massage the scalp for 5 minutes. After using it for 2 months (once a day), evaluate from five aspects: hair loss improvement, scalp moisturization, scalp refreshment, scalp acne improvement, and hair frizz improvement.

Evaluation criteria: 1 point - poor, 2 points - poor, 3 points - average, 4 points - good, 5 points - very good.

Evaluation results: see Table 13.

Table 13 Evaluation Results

From the results in Table 13, it can be seen that the degree of improvement in hair loss, refreshment, scalp acne and hair frizz in Example 1 is significantly better than that in Comparative Example 1. It shows that adding the traditional Chinese medicine composition of the present invention can improve hair loss, improve hair frizz, improve scalp acne, and is not greasy at the same time, and plays the role of scalp care and anti-hair loss.

The above-mentioned embodiment is a preferred embodiment of the present invention, but the embodiment of the present invention is not limited by the above-mentioned embodiment, and any other changes, modifications, substitutions, combinations, Simplifications should be equivalent replacement methods, and all are included in the protection scope of the present invention.

Claims (10)

1. An anti-hair loss Chinese medicine composition, characterized in that it is made of the following components by weight: 5 to 50 parts of Shegan; 5 to 35 parts of Hibiscus bark; 5 to 35 parts of three needles; 5 to 35 parts of licorice. 2. A kind of anti-hair loss traditional Chinese medicine composition according to claim 1, is characterized in that, is made of the following components by weight: Shegan 10～45 parts; Hibiscus bark 7～30 parts; Three needles 7～30 parts 5-30 parts of Xu Changqing; 5-30 parts of licorice. 3. A kind of Chinese medicine composition for preventing hair loss according to claim 2, characterized in that, it is made of the following components by weight: 15-40 parts of shegan; 10-25 parts of hibiscus bark; 10-25 parts of three needles 10-30 parts of Xu Changqing; 10-25 parts of licorice; The anti-hair loss traditional Chinese medicine composition can also be made of the following components by weight: 25-30 parts of shegan; 15-20 parts of hibiscus bark; 15-20 parts of three needles; 18-25 parts of Xu Changqing; share. 4. The preparation method of a kind of anti-hair loss Chinese medicine composition according to any one of claims 1 to 3, characterized in that: comprising the steps of: ①Ingredients: Remove impurities from Shegan, Tujinpi, Sanjiazhen, Xu Changqing and Licorice respectively, wash, dry, crush, pass through a 40-100 mesh sieve, and then mix to obtain a Chinese medicine mixture; ②Extraction: Mix the Chinese medicine mixture and 1,3-butanediol evenly, soak, then extract the mixture of Chinese medicine mixture and 1,3-butanediol through a micro-fluidic extractor, centrifuge, filter, discard the precipitate, and collect filtrate; ③ Concentration: Take the filtrate and concentrate it with a membrane to obtain the traditional Chinese medicine composition. 5. The preparation method of a kind of anti-hair loss Chinese medicine composition according to claim 4, characterized in that, the Chinese medicine mixture and 1,3-butanediol in step ② are in a weight ratio of 1:1 to 15, and the soaking time 1 to 5 hours, the centrifugal speed is 1000 to 3000 rpm, and the centrifugation time is 0.5 to 10 minutes; The filtrate described in step ③ is concentrated to 1 to 3 times the weight of the traditional Chinese medicine mixture in step ② with a membrane; Steps ①～③ were carried out at room temperature. 6. The application of a kind of anti-hair loss Chinese medicine composition in cosmetics according to any one of claims 1 to 3, characterized in that, the anti-hair loss Chinese medicine composition is used to prepare cosmetics, wherein the anti-hair loss Chinese medicine composition accounts for 10% of the weight of the cosmetics 0.1-60%; the anti-hair loss traditional Chinese medicine composition accounts for 1-30% of the weight of the cosmetic. 7. An anti-hair loss cosmetic, characterized in that, its composition contains a kind of anti-hair loss Chinese medicine composition according to any one of claims 1 to 3, and the weight of the anti-hair loss Chinese medicine composition in the anti-hair loss cosmetic is The content is 0.1-60%; the weight content of the anti-hair loss traditional Chinese medicine composition in the anti-hair loss cosmetic is 1-30%. 8. A hair care anti-loss nutritional water, characterized in that, consists of the following components in parts by mass: The anti-hair loss Chinese medicine composition is a kind of anti-hair loss Chinese medicine composition described in any one of claims 1-3. 9. A scalp care anti-shedding shampoo, characterized in that, consists of the following components in parts by mass: The anti-hair loss Chinese medicine composition is a kind of anti-hair loss Chinese medicine composition described in any one of claims 1-3. 10. A scalp care anti-hair loss conditioner, characterized in that, consists of the following components in parts by mass: The anti-hair loss traditional Chinese medicine composition is the anti-hair loss traditional Chinese medicine composition according to any one of claims 1-3; the mass ratio of cetyl alcohol to stearyl alcohol in the cetostearyl alcohol is 3:7.