CN110200870A - A kind of reparation moisture retention water containing solanum lyratum and Flos Hibisci extract - Google Patents

Abstract

The invention discloses a repairing and moisturizing water containing extracts of vine and hibiscus flowers and a preparation method thereof. The repairing and moisturizing water contains extracts of vines and hibiscus flowers, propylene glycol, glycerin, β-glucan, Allantoin, Jojoba Oil, Disodium EDTA, Citric Acid, Phenoxyethanol, Deionized Water. The functional ingredients are extracted from white hair vine and hibiscus flower. The repairing and moisturizing water containing the extracts of vine and hibiscus provided by the invention has certain effects of moisturizing, repairing the skin barrier, anti-oxidation, and antibacterial; meanwhile, the production process of the invention is simple, and there is no greasy feeling after application, and it is easy to Good absorption, moisturizing and water-locking effect, making the skin moisturized for a long time. The white hair vine extract and hibiscus flower extract used have good biological activity, mild and non-irritating.

Description

A Restorative Moisturizer with Extracts of Albicans and Hibiscus Flowers

technical field

The invention belongs to the technical field of cosmetics, and in particular relates to a repairing and moisturizing water containing extracts of vine and hibiscus flower.

Background technique

The skin is located in the outermost layer of the human body surface and has barrier, absorption, secretion, excretion, metabolism, immunity, temperature regulation and sensory functions, among which the skin barrier function is the basis. In 1983, Professor Peter M.Elias of the University of California (the founder of the skin brick wall structure) described the structure and function of the epidermal permeability barrier in detail, and proposed that the stratum corneum located in the outermost layer of the skin plays an important role in the epidermal permeability barrier, and vividly It is described as a "brick wall structure", that is, the corneocytes in the stratum corneum make up the bricks in the "brick wall structure"; the intercellular lipids make up the mortar in the "brick wall structure" The multilayer lamellar membrane structure composed of cells intercalated with intercellular lipids maintains our normal epidermal permeability barrier function. Externally, it can resist the invasion of antigenic substances, microorganisms, sunlight, etc. internally, it can prevent the loss of nutrients and water in the body. The epidermal permeability barrier is damaged, transepidermal water loss (TEWL) increases, and the skin appears dry, desquamated, aging, pigmentation, atopic dermatitis, eczema, psoriasis, ichthyosis, solar dermatitis, etc. Sensitive, irritant dermatitis, hormone-dependent dermatitis and other oily skin, seborrhea diseases such as acne, rosacea, seborrheic dermatitis. At the same time, the ability to resist external antigens and microorganisms decreases, which can lead to the occurrence of various skin diseases. Epidermal permeability barrier, immune barrier, acid barrier and microecology together constitute an overall defense system. The epidermal microecology and pH value can affect the epidermal permeability barrier, and the damage of the epidermal permeability barrier can also affect other barrier functions.

With the improvement of living standards, people pay more and more attention to skin care. However, environmental influences such as ultraviolet radiation and slow metabolism in the human body, imbalance and other reasons lead to various skin problems. According to dermatologists, the water in the baby accounts for more than 80% of the body weight, and the water in the body will decrease with age. Because the baby has enough water, the skin will appear delicate and shiny. In fact, adults have enough sebum secretion, but the water content of adult women's skin is only 65% or less (of which keratin is only 15%), which is far lower than that of babies. Therefore, lack of water is the root of all skin problems.

There are three reasons for water evaporation: high temperature, dryness and wind, and the moisture of the skin is no exception. Therefore, the climate characteristics of the four seasons more or less include these factors, so it is necessary to replenish water and moisturize all the year round. The above three reasons are only environmental factors. The loss of moisture from the skin also includes the problems of the skin itself. In addition, urban life is stressful, fatigue, overtime, staying up late, and unreasonable diet, which aggravates the water shortage of the skin. The skin will look delicate and smooth because of sufficient moisture. The lack of moisture in the skin will lead to dryness, yellowing, dullness, sagging, wrinkles and other aging phenomena, and it is easy to tighten, and fine lines will appear under the eyes and around the nose. Simultaneously modern people's rhythm of life is fast, work is busy, therefore be badly in need of a kind of easy to use, effective skin moisturizing water to satisfy people's demand. However, no matter what method is used to hydrate the skin, it is very easy to evaporate into the air, and oil is the best moisturizing ingredient, which can keep the skin moisture from losing.

In the field of skin care products, moisturizing has always been a constant topic. How to do a good job of moisturizing is the most important issue in protecting the skin. At present, there are many types of moisturizing water on the market, but the moisturizing effect is not satisfactory. Most of them have high alcohol content and are irritating, so they are not suitable for long-term use. Vegetable oils are different from animal oils in this aspect. Most vegetable oils are liquid at room temperature, and most vegetable oils contain unsaturated fatty acids, trace elements, vitamin E and other substances, which naturally have antioxidant properties. At the same time, plant natural products are mild, non-irritating, and meet the needs of most groups. A large number of clinical studies have also shown that the diversity, tolerance and safety of natural product active ingredients are superior to chemical synthetic products. Therefore, judging from the development trend of global cosmetics in recent years, the popularity of natural cosmetics is on the rise, which brings opportunities for natural cosmetics.

Solanum lyratum Thunb is a perennial herb belonging to the family Solanaceae and has a long history of medicinal use. Compounds isolated from vine include saponins, organic acids, terpenoids, polyphenols, flavonoids, sterols, coumarins, polysaccharides and other types of compounds. The steroidal alkaloids and saponins contained therein have antibacterial activity, among which tomatine has a stronger effect, and has anti-inflammatory, anti-allergic, antipruritic and immunosuppressive effects. In 2001, Wei-Wen Kuo et al. studied the antioxidant properties of the crude extract of Baiying to protect epidermal cells. The results showed that the crude extract of Baiying could clear DPPH and delay the oxidation of low-density lipoprotein in a dose-dependent manner. Colleagues also Attenuated the level of reactive oxygen species induced by oxidized LDL and decreased the expression of endothelial oxidative protease.

Hibiscus syriacus is a deciduous shrub or small tree belonging to the genus Malvacea hibiscus. It is a common shrub flower species in gardens, widely distributed in tropical and subtropical regions of my country. Hibiscus flower contains vegetable oil, essential amino acid, polysaccharide, and polyol mucilage, which has the functions of moisturizing, anti-oxidation and scavenging free radicals. The original species of hibiscus had the highest content of total phenols and total flavonoids, and the strongest antioxidant activity. The paper "Preliminary Study on Biological Activity of Hibiscus Flower" by Jin Yueting et al. showed that 1g of Hibiscus Flower is equivalent to 5.19mg of vitamin C and has the ability to remove DPPH; while its antioxidant capacity is equivalent to 9.12mg of vitamin C. The hibiscus flower extract has a certain inhibitory effect on both bacteria and mold, and the inhibitory effect on bacteria is obviously greater than that on mold. Its root bark has cyclic peptides and proanthocyanidins, so it also has bactericidal and disinfecting effects. Folks use this flower juice to wash their faces and improve their beauty. Add appropriate amount of glycerin, emulsifier, vitamin E, etc. to hibiscus flower juice to make an oil-in-water (O/W) skin care lotion, which is suitable for neutral skin, especially for middle-aged and elderly people with age spots on their hands. At the same time, it has the effect of lubricating and moisturizing, which makes up for the shortcomings of the lack of biological active ingredients and trace elements in ordinary skin care lotions. The proanthocyanidins contained in hibiscus can scavenge water-soluble and fat-soluble free radicals in cell membranes, thus inhibiting the process of releasing certain enzymes to damage capillary walls. Proanthocyanidins are attached to collagen, which can prevent the damage of enzymes that destroy collagen. Proanthocyanidins not only help collagen fibers form a cross-linked structure, but also help restore damage caused by excessive cross-linking caused by injuries and free radicals. Excessive cross-linking suffocates and hardens connective tissue, which wrinkles and prematurely ages the skin. Anthocyanins also protect the body from sun damage and promote the healing of psoriasis and age spots. Proanthocyanidins are also excellent additions to topically applied skin creams. It can make the skin rejuvenated and healthy, the cells are full of vitality, restore its stretch and elasticity, and repair the skin.

Aiming at the phenomenon of skin sensitivity and dehydration, many anti-allergic and repairing skin care products have appeared, mainly including anti-allergic skin care products, mainly by reducing stimulating ingredients such as spices, pigments, alcohol and preservatives, or adding raw materials such as aloe vera extract and chamomile extract Achieve anti-allergic effect; in addition, there are added hormones, which can repair the skin to a certain extent after skin sensitivity occurs. It can be seen from the above that the existing skin care products have single effects, and cannot achieve the dual effects of anti-allergic and repairing for sensitive skin, and the repair cycle is long; they even rely on hormone-containing products.

Chinese patent application CN 106236696 B (application number 201610744048.2) discloses a soothing moisturizing water containing chlorophyll and a preparation method thereof. However, there are problems such as unstable chlorophyll and cumbersome extraction process, and the efficacy needs to be investigated. Chinese patent application CN102000011A (application number CN201010561334) discloses a hibiscus flower skin care lotion and a preparation method thereof. However, in the extraction process, the extract contains many impurities and the extraction efficiency is low. After searching, it has not been found that there is any application of the compound of the extract of the white hair vine and the extract of the hibiscus flower in moisturizing cosmetics.

Since the crude extract of natural products contains a variety of organic compounds, and the biological activity content is low, unstable, and difficult to separate and purify, for plants containing saponins, it is easy to decompose them by ordinary water extraction, and decompression Filtration produces a lot of foam and is difficult to concentrate, so more efficient purification methods are required to obtain pure extracts.

Contents of the invention

In order to solve the above-mentioned problems, the present invention provides a repairing moisturizing water containing extracts of vine and hibiscus, which is mild and skin-friendly, easy to absorb, safe and non-irritating, has good moisturizing and soothing effects, and has a stable system.

The purpose of the present invention is achieved through the following technical solutions:

A kind of repairing moisturizing water containing the extracts of Alba vine and hibiscus flower, which is made of the following components by weight:

Further, the preparation method of the vine extract is as follows:

(1) Take a certain amount of white hair rattan, wash and dry it, grind it into powder, and get the white hair rattan powder through a 60-mesh sieve, and take 20 to 50 parts by weight;

(2) Disperse the white hair rattan powder in deionized water, stir and mix thoroughly to obtain the white hair rattan suspension; the mass ratio of the white hair rattan powder to the deionized water is 1:10～20;

(3) Heating the suspension of rattan to 40-55° C., then adding 1.0-1.5 parts by weight of cellulase, stirring, and fully reacting for 2-3 hours;

(4) Add 0.6-1.2 parts by weight of pectinase to the mixed solution in step (3), stir, and fully react for 1-2h;

(5) The temperature of the mixed solution in step (4) is lowered to 35-50° C., adjusted to 4-5 with a pH regulator, and filtered to remove the filter residue;

(6) Concentrate the filtrate to 120-150 parts by weight to obtain the extract of Rattan alba.

Further, the preparation method of the hibiscus flower extract is as follows:

A1. Wash and drain the freshly picked hibiscus flowers, separate the petals, and freeze-dry them at low temperature;

A2. Weigh 20-40 parts by weight of the dried hibiscus flower, grind it into powder, and pass through a 40-mesh sieve to obtain the hibiscus flower powder;

A3. Add 3 times the weight of hibiscus flower powder to soak in deionized water, add pH regulator to adjust pH to 4, add 0.5-1 weight part of cellulase, stir well, put in 40-50°C constant temperature water bath for 1.5h, add hibiscus The flower powder is 5 times the weight of the ethanol solution with a mass concentration of 75% and is refluxed for 1 to 2 hours, and filtered while it is hot to obtain the first extract and medicinal residues;

A4. Adding 5 times the weight of the medicinal residues to the medicinal residues with an ethanol solution with a mass concentration of 50% for reflux extraction for 0.5 to 1 hour, and filtering while hot to obtain a second extract;

A5. Mix the first extract and the second extract, evaporate, recover ethanol, centrifuge, take the supernatant, extract with n-butanol for 3 times, and volatilize n-butanol to obtain hibiscus flower extract.

Further, the pH regulator is 2.5mol/L hydrochloric acid solution.

The present invention also includes a preparation method of repairing and moisturizing water containing the extracts of vine and hibiscus flower, comprising the following steps:

S1. Add propylene glycol, glycerin, β-glucan, allantoin, water-soluble jojoba oil, citric acid and disodium edetate into deionized water, and heat to 70-80°C. Stir for 20-30 minutes until uniform, and obtain material A;

S2. Cool down the material A to 30-40°C, add phenoxyethanol, white hair vine extract and hibiscus flower extract, continue to stir until uniform, and obtain material B;

S3. Material B is cooled to -5~-10°C, kept warm for 30~60 minutes, filtered, and finally sterilized to obtain repairing moisturizing water.

Beneficial effects of the present invention:

1. The present invention uses natural plants, which have both nutritional and pharmacological effects, do not contain hormones, do not contain harmful ingredients such as heavy metals, are non-toxic and have no side effects, and meet the requirements of long-acting cosmetics and adding natural ingredients.

2. The extract of white hair vine has anti-oxidation, anti-inflammatory, anti-bacterial, anti-allergic effects; at the same time, hibiscus flower contains flavonoids such as proanthocyanidins and polysaccharides, which have moisturizing effect, can effectively promote blood circulation, dilute pigmentation, and nourish the skin. Increase skin elasticity, and has anti-radiation function.

3. The present invention has the effects of moisturizing, locking skin moisture, activating skin, antibacterial, anti-inflammatory, anti-allergic, and repairing skin, and is mild and does not irritate the skin.

4. The cost of the present invention is moderate, and it is suitable for various types of skin. Especially sensitive skin and skin with compromised skin barrier.

5. The method of the present invention is simple and easy to implement; by adopting enzyme extraction method and freeze-drying technology, at low temperature, the biological activity can be maintained to a certain extent, preventing loss, improving the yield and effectiveness, good purification effect, and solving the problem of biological activity. Components are easily decomposed and inactivated during processing. Using enzyme extraction method, the reaction is mild.

Detailed ways

In order to better understand the present invention, the present invention will be further described by the following examples, which are only used to explain the present invention and do not constitute any limitation to the present invention.

The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are all commercially available products.

Example 1

1) Recipe

A repairing and moisturizing water containing extracts of vine and hibiscus consists of the following components in parts by weight:

Alba vine extract: 10 parts; Hibiscus flower extract: 10 parts; Propylene glycol: 1 part; Glycerin: 2 parts; β-glucan: 1 part; Allantoin: 1 part; Water-soluble jojoba oil: 0.3 EDTA disodium: 0.0008 parts; citric acid: 0.3 parts; phenoxyethanol 0.2 parts; deionized water 74.1992 parts.

2) Preparation

The extract of the vine extract is prepared according to the following method:

(1) the white hair rattan is washed and dried, and it is ground into powder, and the white hair rattan powder is crossed through a 60-mesh sieve, and 20 parts by weight are weighed;

(2) Disperse the white hair rattan powder in deionized water, stir and mix thoroughly to obtain the white hair rattan suspension; the mass ratio of the white hair rattan powder to the deionized water is 1:10;

(3) Heating the suspension of rattan to 40°C, then adding 1.0 parts by weight of cellulase, stirring, and fully reacting for 2 hours;

(4) Add 0.6 parts by weight of pectinase to the mixed solution of step (3), stir, and fully react for 1 hour;

(5) The temperature of the mixed solution in step (4) is lowered to 35° C., the pH is adjusted to 4 to 5 with 2.5 mol/L hydrochloric acid, filtered, and the filter residue is removed;

(6) Concentrate the filtrate to 120 parts by weight to obtain the extract of Ceruleus albicans.

The preparation method of the hibiscus flower extract is as follows:

(1) Wash and drain the freshly picked hibiscus flowers, separate the petals, and freeze-dry them at low temperature;

(2) Take 20 parts by weight of dried hibiscus, put it into a pulverizer and grind it into powder, and cross a 40-mesh sieve to obtain hibiscus powder;

(3) Add 3 times the weight of hibiscus flower powder for soaking in deionized water, add 2.5mol/L hydrochloric acid to adjust the pH to 4, add 0.5 parts by weight of cellulase, stir well, put it in a constant temperature water bath at 40°C for 1.5h, add hibiscus flower 5 times the mass concentration of the powder is 75% ethanol solution for reflux extraction for 1 hour, and filtered while hot to obtain the first extract and dregs;

(4) Adding 5 times the mass concentration of the medicinal residues to the medicinal residues is reflux extraction of 50% ethanol solution for 0.5h, and filtering while hot to obtain the second extract;

(5) Mix the first extract and the second extract, evaporate with a rotary evaporator, recover ethanol, centrifuge, take the supernatant, extract with n-butanol for 3 times, volatilize n-butanol, and obtain the hibiscus flower extract .

The described repairing and moisturizing water containing the extracts of vine and hibiscus is prepared according to the following method:

(1) Add propylene glycol, glycerin, β-glucan, allantoin, water-soluble jojoba oil, citric acid and disodium edetate into deionized water, and heat to 70°C. 20min to uniformity, to obtain material A;

(2) Cool down the material A to 30°C, add phenoxyethanol, white hair vine extract and hibiscus flower extract, continue to stir until uniform, and obtain material B;

(3) Material B was cooled to -5°C, kept warm for 30 minutes, filtered, and finally sterilized to obtain repairing moisturizing water.

Example 2

1) Recipe

A repairing and moisturizing water containing extracts of vine and hibiscus consists of the following components by weight:

Alba vine extract: 15 parts; hibiscus flower extract: 20 parts; propylene glycol: 2 parts; glycerin: 2 parts; β-glucan: 1 part; allantoin: 1 part; water-soluble jojoba oil: 0.3 0.002 parts of disodium EDTA; 1 part of citric acid; 0.2 parts of phenoxyethanol; 57.498 parts of deionized water.

2) Preparation

The extract of the vine extract is prepared according to the following method:

(1) Take the white hair rattan, wash and dry it, grind it into powder, and pass through a 60-mesh sieve to get the white hair rattan powder, and take 20 to 50 parts by weight;

(2) Disperse the white hair rattan powder in deionized water, stir and mix thoroughly to obtain the white hair rattan suspension; the mass ratio of the white hair rattan powder to the deionized water is 1:10-1:20. ;

(3) Heating the suspension of rattan to 40-55° C., then adding 1.0-1.5 parts by weight of cellulase, stirring, and fully reacting for 2-3 hours;

(4) Add 1.2 parts by weight of pectinase to the mixed solution of step (3), stir, and fully react for 1-2h;

(5) The temperature of the mixed solution in step (4) is lowered to 35-50° C., the pH is adjusted to 4-5 with 2.5 mol/L hydrochloric acid, filtered, and the filter residue is removed;

(6) Concentrate the filtrate to 150 parts by weight to obtain the Rhizoma alba extract.

The preparation method of the hibiscus flower extract is as follows:

(1) Wash and drain the freshly picked hibiscus flowers, separate the petals, and freeze-dry them at low temperature;

(2) Take by weighing 40 parts by weight of dried hibiscus, put it into a pulverizer and grind it into powder, and cross a 40-mesh sieve to obtain hibiscus powder;

(3) Add 3 times the weight of hibiscus flower powder to soak in deionized water, add 2.5mol/L hydrochloric acid to adjust the pH to 4, add 1 part by weight of cellulase, stir well, put it in a constant temperature water bath at 40-50°C for 1.5h, add Hibiscus flower powder 5 times the mass concentration of 75% ethanol solution for reflux extraction for 1 hour, filtered while hot, to obtain the first extract and dregs;

(4) Adding 5 times the mass concentration of the medicinal residues to the medicinal residues is reflux extraction of 50% ethanol solution for 1 hour, and filtering while hot to obtain the second extract;

(5) The two filtrates were combined, evaporated by a rotary evaporator, recovered ethanol, centrifuged, used for supernatant, extracted with n-butanol for 3 times, and volatilized n-butanol to obtain hibiscus flower extract.

The described repairing and moisturizing water containing the extracts of vine and hibiscus is prepared according to the following method:

(1) Add propylene glycol, glycerin, β-glucan, allantoin, water-soluble jojoba oil, citric acid and disodium edetate into deionized water, and heat to 80°C. 30min to uniformity, to obtain material A;

(2) Cool down the material A to 40°C, add phenoxyethanol, extracts of the white hair rattan and hibiscus flower extract, continue to stir until uniform, and obtain the material B;

(3) Material B is cooled to -5～-10°C, kept warm for 60 minutes, filtered, and finally sterilized to obtain repairing moisturizing water.

Example 3

1) Recipe

A repairing and moisturizing water containing extracts of vine and hibiscus consists of the following components by weight:

Alba vine extract: 15 parts; Hibiscus flower extract: 25 parts; Propylene glycol: 3 parts; Glycerin: 1 part; β-glucan: 1 part; Allantoin: 2 parts; Water-soluble jojoba oil: 0.4 0.003 parts of disodium EDTA; 2 parts of citric acid; 0.2 parts of phenoxyethanol; 54.498 parts of deionized water.

2) Preparation

The extract of the vine extract is prepared according to the following method:

(1) Take the white hair rattan, wash and dry it, grind it into powder, and pass through a 60-mesh sieve to get the white hair rattan powder, and take 20 to 50 parts by weight;

(2) Disperse the white hair rattan powder in deionized water, stir and mix thoroughly to get the white hair rattan suspension; the mass ratio of the white hair rattan powder to the deionized water is 1:10～1:20;

(3) Heating the suspension of rattan to 40-55° C., then adding 1.0-1.5 parts by weight of cellulase, stirring, and fully reacting for 2-3 hours;

(4) Add 1.2 parts by weight of pectinase to the mixed solution of step (3), stir, and fully react for 1-2h;

(5) The temperature of the mixed solution in step (4) is lowered to 35-50° C., the pH is adjusted to 4-5 with 2.5 mol/L hydrochloric acid, filtered, and the filter residue is removed;

(6) Concentrate the filtrate to 150 parts by weight to obtain the Rhizoma alba extract.

The preparation method of the hibiscus flower extract is as follows:

(1) Wash and drain the freshly picked hibiscus flowers, separate the petals, and freeze-dry them at low temperature;

(2) Take by weighing 40 parts by weight of dried hibiscus, put it into a pulverizer and grind it into powder, and cross a 40-mesh sieve to obtain hibiscus powder;

(3) Add 3 times the weight of hibiscus flower powder to soak in deionized water, add 2.5mol/L hydrochloric acid to adjust the pH to 4, add 1 weight part of cellulase, stir well, put it in a constant temperature water bath at 40-50°C for 1.5h, add Hibiscus flower powder 5 times the mass concentration of 75% ethanol solution for reflux extraction for 1 hour, filtered while hot, to obtain the first extract and dregs;

(4) Adding 5 times the mass concentration of the medicinal residues to the medicinal residues is reflux extraction of 50% ethanol solution for 1 hour, and filtering while hot to obtain the second extract;

(5) The two filtrates were combined, evaporated by a rotary evaporator, ethanol was recovered, centrifuged, the supernatant was taken, extracted with n-butanol three times, and n-butanol was volatilized to obtain hibiscus flower extract.

The described repairing and moisturizing water containing the extracts of vine and hibiscus is prepared according to the following method:

(1) Add propylene glycol, glycerin, β-glucan, allantoin, water-soluble jojoba oil, citric acid and disodium edetate into deionized water, and heat to 80°C. 30min to uniformity, to obtain material A;

(2) Cool down the material A to 40°C, add phenoxyethanol, extracts of the white hair rattan and hibiscus flower extract, continue to stir until uniform, and obtain the material B;

(3) Material B is cooled to -5～-10°C, kept warm for 60 minutes, filtered, and finally sterilized to obtain repairing moisturizing water.

Test Example 1 Sensory Test

1.1 Appearance: Take the sample and observe it visually at room temperature and without direct sunlight.

1.2 Fragrance: First put the same amount of samples and specified samples (according to the internal regulations of the enterprise) in the same container, use absorbent paper with a width of 0.5cm to 1.0cm and a length of 10cm to 15cm as the fragrance evaluation paper, and dip them in Take the sample and the specified sample about 1cm-2cm (the two should be close to each other), and use the sense of smell to identify.

Table 1. Sensory test results:

Test example 2 physical and chemical index inspection

2.1 pH: Measured according to the method specified in GB/T135531.1 (direct measurement method).

2.2 heat resistance

2.2.1 Instrument: constant temperature incubator: temperature control accuracy ± 1 ℃.

2.2.2 Operating procedures: adjust the constant temperature incubator to (40±1)°C in advance, and place a bottle of the packaged complete sample in the constant temperature incubator. Take it out after 24 hours, and observe it visually after returning to room temperature.

2.3 cold resistance

2.3.1 Instrument: constant temperature incubator: temperature control accuracy ± 1 ℃.

2.3.2 Operating procedures: adjust the constant temperature incubator to (5±1)°C in advance, and place a bottle of the fully packaged sample in the constant temperature incubator. Take it out after 24 hours, and observe it visually after returning to room temperature.

Table 2. Physical and chemical test results:

Test Example 3 Product skin irritation/corrosion test

According to " Hygienic Standards for Cosmetics " (2015 edition) skin irritation/corrosion test carries out skin reaction score to the repairing moisturizing water containing the extracts of vine and hibiscus flower of embodiments 1 to 3, and tests whether the present invention has local effects on mammalian skin Irritation or corrosion and its degree. The specific operation is as follows:

3.1 Tested substance: the repairing and moisturizing lotion containing the extracts of vine and hibiscus flower in Examples 1-3, the original solution can be used directly without dilution.

3.2 Experimental animals and feeding environment

The first choice is 4 adult, healthy white rabbits with no skin damage, both female and male, but the female animals should be non-pregnant and have never given birth. The experimental animals should be kept in single cages, and the animals should be acclimatized in the environment of the experimental animal room for at least 3 days before the experiment.

Experimental animals and experimental animal rooms comply with the corresponding national regulations. Standard compound feed was selected, and drinking water was not restricted.

3.3 Acute skin irritation test procedure

3.3.1 About 24 hours before the test, cut off the hair on both sides of the back and spine of the experimental animal, without damaging the epidermis, and the left and right sides of the hair removal area are about 3cmx3cm each.

3.3.2 Take about 0.5mL (g) of the repairing and moisturizing water containing the extracts of vine and hibiscus in Examples 1-3 and apply it directly on the skin, and then use two layers of gauze (2.5cmx2.5cm) and a layer of cellophane or similar Cover with non-irritating adhesive tape and bandages. The skin on the other side served as a control. A closed test is used. Apply once a day for 14 days. From the second day on, the hair was clipped before each application, and the residual test substance was removed with water. After 1 hour, observe the skin reaction at the application site, and score the skin reaction according to Table 3 and 4, and treat the control area and the test area in the same way.

3.3.3 Result evaluation: Calculate the average score of each animal per day according to the following formula. Table 5 was used to determine the intensity of skin irritation.

Average score per animal per day = (∑ erythematous edema score/number of animals tested)/14

Skin irritation evaluation criteria:

Table 3 Evaluation criteria for skin erythema and eschar

skin reaction integral no erythema 0 slight erythema 1 obvious erythema 2 Moderate to severe erythema 3 Severe erythema (purple) to mild eschar formation 4 highest score 4

Table 4 Skin edema evaluation criteria

Table 5 Skin irritation evaluation criteria

integral Stimulus intensity standard 0～0.5 non-irritating 0.5～2.0 mild irritant 2.0～6.0 Moderate Irritation Meter 6.0～8.0 strong irritant

Table 6 Skin irritation intensity score results

Conclusion: Known from Table 6, there is almost no erythema formation and edema in the test area and the control area, indicating that the repairing and moisturizing water containing the extracts of vine and hibiscus has no irritation to the skin of white rabbits.

Test Example 4 Human Controlled Test

Carry out skin stimulation test to the present invention, choose 6 adult females with healthy skin to do skin stimulation test, forearm medial area is test site, one side is experiment area, the other side is control area, take three pieces of cotton pads, apply respectively It contains 0.05g of white hair rattan and hibiscus flower moisturizing water prepared in Example 1, 0.05g of Epeilan barley water, and 0.05g of Winona Shumin moisturizing lotion, and sticks it on the experimental area on the inner side of the forearm, and fixes it with medical adhesive tape. Applicable persons do not eat spicy food or irritating food, and the experimental site does not touch water. 24h to uncover the observation. The observation results are shown in Table 7.

Table 7. Skin test results of the repairing moisturizing water containing the extracts of vine and hibiscus:

Test results: There was no significant difference in the results of the experimental group's repairing moisturizing water containing the extracts of Alba vine and hibiscus flower, and the results of the control group's marketed barley water and Winona Shumin moisturizing lotion, and there was no difference between the results of the control group.

Test Example 5 Product Efficacy Evaluation

5.1 Moisturizing effect test: Determination of water loss rate experiment by gravimetric method

5.1.1 Preparation before the test The gravimetric method to determine the water loss rate of the humectant must be carried out in a constant temperature and humidity environment.

5.1.2 One day before the experiment, place a certain volume of saturated potassium carbonate solution at the bottom of the desiccator to make the liquid depth greater than 1cm, place a porous partition on it, cover it with a lid, keep it sealed, and place it in an environment of 25°C. After the partial pressure of water vapor in the air is constant, it can maintain a relative humidity of about 43%.

5.1.3 The repairing and moisturizing water containing the extracts of vine and hibiscus in Examples 1 to 3 was used as the sample to be tested.

5.1.4 Weigh a certain amount of sample, and evenly coat it on a glass plate with 3M adhesive tape (5cm×5cm relative humidity. The amount of sample is about 2mg/cm2, and place it in a container filled with a saturated aqueous solution of potassium carbonate (RH43%) In the desiccator, weigh the mass of the sample after standing for a period of time (2h, 4h, 6h, 8h), perform three experiments in parallel, and take the average value.

5.1.5 Calculate the water loss rate.

5.1.6 Water loss rate

M ₁ - the mass of the sample before placement;

M ₂ - the mass of the sample after standing.

5.1.7 The results of water loss rate are shown in Table 7.

Table 7. Water loss test results

time 2 hours 4h 6 hours 8 hours Example 1 5% 12% 17% 35% Example 2 7% 11% 16% 38% Example 3 5% 11% 13% 36%

5.2 Antibacterial effect test:

5.2.1 Bacterial culture: Take Staphylococcus aureus, Escherichia coli, Salmonella, Pseudomonas aeruginosa, Candida albicans and other fresh strains before the test, and inoculate them into nutrient broth medium and modified Martin medium After incubating at a suitable temperature for a suitable time, dilute it with normal saline to a suitable concentration, measure the number of viable bacteria, and set aside.

5.2.2 During the test, take several sterilized small test tubes, and dilute the repairing moisturizing water containing the extracts of vine and hibiscus flower in Example 1 with the corresponding medium to 100, 50, 25, 12.5, 6.25, 3.12, 1.56 respectively , 0.78mg/ml into 1ml of each tube, take 0.1ml of appropriate dilution grade bacteria solution in each antibacterial test tube, the inoculation amount is 105 test bacteria, shake well, make the test bacteria fully contact with the medicine solution, place in 37 Cultivate in the incubator for 16-24 to observe the growth of the test bacteria. The minimum inhibitory concentration of the drug is defined as the lowest drug concentration at which bacteria do not grow.

5.2.3 The antibacterial results are shown in Table 8.

Table 8. Determination of the antibacterial effect of the repairing moisturizing water containing the extracts of Alba vine and hibiscus:

Remarks: -- indicates no bacteria growth, + indicates a small amount of bacteria growth, ++ indicates moderate bacteria growth, and +++ indicates a large number of bacteria growth.

Table 9. The results of antibacterial and antibacterial zones of the repairing moisturizing water containing the extracts of vine and hibiscus:

strain name Escherichia coli Staphylococcus aureus Bacillus subtilis Inhibition diameter 8.02mm 12.01mm 11.02mm

Known from Table 8, the repairing moisturizing water containing the extracts of Alba vine and hibiscus is 100mg/ml to Staphylococcus aureus, Escherichia coli, Salmonella, Candida albicans minimum inhibitory concentration (MIC), and is 100mg/ml to Pseudomonas aeruginosa The minimum inhibitory concentration (MIC) of monocellular bacteria is 50mg/ml.

According to the judgment standard of the antibacterial circle test: the antibacterial circle diameter greater than 20mm is extremely sensitive; 15-20mm is highly sensitive; 10-15mm is moderately sensitive; 7-9mm is low-sensitive; less than 7mm is not sensitive. It can be seen from Table 9 that the repairing moisturizing water containing the extracts of Alba vine and Hibiscus flower has certain antibacterial properties against the above three common bacteria.

Test Example 6 Evaluation of Human Experience

Invite 90 female volunteers with healthy skin between the ages of 20 and 50, and randomly divide them into 9 groups, 20 people in each group, with an average age of 22 ± 2 years old, try the moisturizing water prepared in Examples 1 to 3 respectively, observe and Feel the effect of use. How to use: After the volunteer cleans the face, pour about 3mL of the above moisturizing water on the cotton pad, moisten the cotton pad without dripping, and determine the direction of the cotton pad so that the fiber direction of the cotton pad is perpendicular to the finger, put the cotton pad All the tablets cover the skin surface, gently press with your fingers, and wipe gently, so that the repairing moisturizing water penetrates into the skin, after wiping, use the way of patting to speed up the penetration, use it once a day in the morning and evening, insist on using it for a month, every week Check at least once for systemic and local adverse reactions, and record the results in detail. The test evaluation results are shown in Table 10:

Table 10. Feedback summary table for the trial (one month) of the repairing moisturizing water containing the extracts of Alba vine and Hibiscus flower

It can be seen from Table 10 above that:

The invention is widely loved and recognized by the trial volunteers. The trial volunteers said that the repairing moisturizing water is warm, skin-friendly, non-irritating, and easy to absorb. After use, the face is refreshing and not greasy. After a month of trial, the skin feels dry, rough, and red. Problems such as bloodshot eyes have been significantly improved, pores have been reduced, and the skin has become smooth, hydrated and elastic.

At the same time, none of the trial volunteers experienced allergies, nor did they experience skin itching, tingling, redness and swelling.

Therefore, a kind of repairing and moisturizing water containing the extracts of vine and hibiscus of the present invention is warm and safe, and can be used for a long time.

The above-mentioned embodiments only illustrate the principles and effects of the present invention, but are not intended to limit the present invention. Anyone skilled in the art can modify or change the above-mentioned embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or changes made by those skilled in the art without departing from the spirit and technical ideas disclosed in the present invention should still be covered by the claims of the present invention.

Claims (5)

1. a kind of repairing moisture-retaining water that contains the extracts of vine and hibiscus, is characterized in that, is made of the component of following weight part: 10~20 parts of white hair rattan extract; Hibiscus flower extract 10~20 parts; 1~3 parts of propylene glycol; 1~3 parts of glycerin; 1~3 parts of β-glucan; Allantoin 1~3 parts; 0.2~0.5 parts of water-soluble jojoba oil; Disodium edetate 0.0008-0.002 parts; 0.3~1 part of citric acid; Phenoxyethanol 0.1~0.4 parts; The rest is deionized water, and the total amount is 100 parts. 2. according to claim 1, a kind of repairing moisturizing water containing vine and hibiscus flower extract, is characterized in that, the preparation method of described vine extract is as follows: (1) Wash and dry the white hair rattan, grind it into powder, pass through a 60-mesh sieve to get the white hair rattan powder, and weigh 20 to 50 parts by weight; (2) Disperse the white hair rattan powder in deionized water, stir and mix thoroughly to get the white hair rattan suspension; the mass ratio of white hair rattan powder to deionized water is 1:10~20; (3) Heating the suspension of the white hair rattan to 40-55°C, then adding 1.0-1.5 parts by weight of cellulase, stirring, and fully reacting for 2-3 hours; (4) Add 0.6-1.2 parts by weight of pectinase to the mixed solution in step (3), stir, and fully react for 1-2 hours; (5) In step (4), lower the temperature of the mixed solution to 35-50°C, adjust the pH to 4-5 with a pH regulator, filter, and remove the filter residue; (6) Concentrate the filtrate to 120-150 parts by weight to obtain the extract of Rattan alba. 3. according to claim 1, a kind of repairing and moisturizing water containing vine and hibiscus flower extract, is characterized in that, the preparation method of described hibiscus flower extract is as follows: A1. Wash and drain the fresh hibiscus flowers, separate the petals, and freeze-dry them at low temperature; A2. Weigh 20-40 parts by weight of the dried hibiscus flower, grind it into powder, and pass through a 40-mesh sieve to obtain the hibiscus flower powder; A3. Add 3 times the weight of hibiscus flower powder to soak in deionized water, add a pH regulator to adjust the pH to 4, add 0.5-1 weight part of cellulase, stir well, put it in a constant temperature water bath at 40-50°C for 1.5 hours, add hibiscus The mass concentration of 5 times the weight of flower powder is 75% ethanol solution, which is refluxed and extracted for 1-2 hours, and filtered while it is hot to obtain the first extract and medicinal residues; A4, adding 5 times the weight of the medicinal residues to the medicinal residues is a 50% ethanol solution for reflux extraction for 0.5 to 1 hour, and filtering while it is hot to obtain the second extract; A5. Mix the first extract and the second extract, evaporate with a rotary evaporator, recover ethanol, centrifuge, take the supernatant, extract with n-butanol three times, and volatilize n-butanol to obtain the hibiscus flower extract. 4. According to claim 2 or 3, a kind of repairing and moisturizing water containing the extracts of vine and hibiscus, it is characterized in that: the pH regulator is 2.5mol/L hydrochloric acid solution. 5. A preparation method for repairing moisture-retaining water containing Alba vine and hibiscus flower extract, is characterized in that, comprises the following steps: S1. Add propylene glycol, glycerin, β-glucan, allantoin, water-soluble jojoba oil, citric acid and disodium edetate into deionized water, and heat to 70-80°C. Stir for 20~30min until uniform, and obtain material A; S2. Cool down the material A to 30-40°C, add phenoxyethanol, white hair vine extract and hibiscus flower extract, continue to stir until uniform, and obtain material B; S3. Material B is cooled to -5~-10°C, kept warm for 30~60 minutes, filtered, and finally sterilized to obtain repairing moisturizing water.