CN109520930A - A kind of elisa assay instrument - Google Patents
A kind of elisa assay instrument Download PDFInfo
- Publication number
- CN109520930A CN109520930A CN201811637624.9A CN201811637624A CN109520930A CN 109520930 A CN109520930 A CN 109520930A CN 201811637624 A CN201811637624 A CN 201811637624A CN 109520930 A CN109520930 A CN 109520930A
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- Prior art keywords
- axis component
- loophole
- elisa assay
- assay instrument
- testing agency
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- 238000003556 assay Methods 0.000 title claims abstract description 28
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 27
- 238000012360 testing method Methods 0.000 claims abstract description 26
- 238000002347 injection Methods 0.000 claims abstract description 23
- 239000007924 injection Substances 0.000 claims abstract description 23
- 238000001514 detection method Methods 0.000 claims abstract description 21
- 230000003287 optical effect Effects 0.000 claims abstract description 18
- 239000000463 material Substances 0.000 claims abstract description 7
- 239000007921 spray Substances 0.000 claims description 6
- 230000008033 biological extinction Effects 0.000 abstract description 3
- 230000035945 sensitivity Effects 0.000 abstract description 3
- 238000004140 cleaning Methods 0.000 description 12
- 238000010586 diagram Methods 0.000 description 6
- 239000000427 antigen Substances 0.000 description 5
- 102000036639 antigens Human genes 0.000 description 5
- 108091007433 antigens Proteins 0.000 description 5
- 238000002965 ELISA Methods 0.000 description 3
- 238000003018 immunoassay Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000011017 operating method Methods 0.000 description 2
- 230000000149 penetrating effect Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Pathology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention discloses a kind of elisa assay instrument, belong to automatic detection system technical field.Elisa assay instrument of the invention is equipped with X-axis component on the rack, is fixedly mounted on X-axis component Z axis component, reagent placement mechanism, wiper mechanism and the chip fixed mechanism arranged in X direction and testing agency；X-axis Component driver Z axis component is mobile in X-direction；Injection mechanism is installed, it is mobile in Z-direction for Z axis Component driver on Z axis component；Reagent placement mechanism, wiper mechanism and chip fixed mechanism are below injection mechanism；Testing agency includes laser emitter, optical signal detector and the first loophole, filter and the second loophole between laser emitter and optical signal detector, and the first loophole and the second loophole are coaxially disposed.The present invention realizes full-automatic detection, and testing agency can utilize the straightline propagation and black material extinction principle of light, and effective exclusive PCR light improves detection sensitivity and detection accuracy.
Description
Technical field
The present invention relates to a kind of elisa assay instrument, belong to automatic detection system technical field.
Background technique
ELISA is a kind of immunoassays (immunoassay, IA), basis be antigen or antibody immobilization and antigen or
The enzyme of antibody marks.After the substrate of enzyme reaction is added, substrate becomes color products by enzymatic, is examined in the amount and sample of product
The amount of substance is directly related, thus carries out qualitative or quantitative analysis.Due to various antigen compositions, the haptens including small molecule,
The antiserum or monoclonal antibody that can be used to prepare specificity just can detect corresponding in sample using this antibody as reagent
Antigen, therefore the application range of immunoassays is extremely wide.
The core of elisa assay instrument is all a colorimeter, and the content of antigen or antibody is analyzed with colorimetric method, further includes
The operating procedures such as sample-adding, heat preservation, washing, colour developing.ELSIA operating procedure is complicated, influences that reaction factor is more, and especially solid phase carries
The coating hardly possible of body reaches consistent between each individual, therefore in quantitative determination, every batch of test must use a series of various concentrations
Reference standard product make standard curve at identical conditions.Existing elisa assay instrument is substantially that the enzyme-linked of separate type is exempted from
Epidemic disease instrument, each step is operated respectively, cumbersome, is easy error, and error influence factor is larger.In addition, existing
Elisa assay instrument optical detection system in, it is ineffective to the exclusion of interference light, be affected to detection sensitivity, or
Person is to need to operate using magazine, cumbersome, and to the more demanding of equipment.
Summary of the invention
In order to solve the above technical problems, the present invention provides a kind of elisa assay instrument, elisa assay instrument of the invention is height
The automated analysis detection device of integrated level has the function of automatic sampling sample-adding, automatic cleaning, temperature automatically controlled, automatic detection etc.,
And the optical path testing agency that the present invention designs can effectively be arranged using the straightline propagation of light and the principle of black material extinction
Except interference light, detection sensitivity and detection accuracy are improved.
The first purpose of the invention is to provide a kind of elisa assay instrument, including rack；
Be equipped in the rack X-axis component, Z axis component, reagent placement mechanism, wiper mechanism, chip fixed mechanism and
Testing agency；
The Z axis component is fixedly mounted on X-axis component, can be mobile in X-direction by X-axis Component driver；The Z axis
Injection mechanism is installed, the injection mechanism can be by the lower movement in z-direction of Z axis Component driver on component；In conjunction with Z axis component
Can be mobile in X-direction by X-axis Component driver, injection mechanism can be moved in X-direction and Z-direction；
Reagent placement mechanism, wiper mechanism and the chip fixed mechanism is mounted on the lower section of injection mechanism, and tries
Agent placement mechanism, wiper mechanism and chip fixed mechanism are arranged in X direction；Injection mechanism can be in reagent placement mechanism and chip
It is moved between fixed mechanism, the operation such as is loaded, is sampled, cleaned；
The testing agency includes laser emitter, optical signal detector and is successively set on laser emitter and light and believes
The first loophole, filter and the second loophole between number detector, first loophole and the second loophole are coaxial
Setting, the testing agency are prepared using black material.
Further, when detection, chip is placed between laser emitter and the first loophole.
Further, the filter is the filter through laser transmitter projects wavelength.
Further, the testing agency further includes laser emitter limiting device, the laser emitter limit
Device includes third loophole and laser emitter limit hole, and the third loophole and the first loophole are coaxially disposed.Swash
The light of optical transmitting set transmitting is projected by third loophole, through chip to be detected, then in turn through the first loophole, is filtered
Piece and second penetrates hole, reaches optical signal detector and is detected.
Further, the reagent placement mechanism includes the first temperature control equipment, orifice plate and setting in reagent orifice plate
On lid；The lid can be mobile in X-direction.Reagent orifice plate can be 96 orifice plates, and the first temperature control equipment can be real
Existing 2 DEG C~25 DEG C of accurate control, realizes the protection to reagent.
Further, the wiper mechanism includes rinse bath and spray equipment.Cleaning solution is pumped into spray equipment, is led to
The mode of partial high pressure spray cleaning is crossed to cleaning outside injection mechanism, while injection mechanism by drawing cleaning repeatedly
Liquid cleans the inside of injection mechanism, and cleaning effect is more preferable, will not impact to reagent and chip to be detected.
Further, the chip fixed mechanism includes wafer support frame, fastener and second temperature control device.
ELISA chip is fixed on wafer support frame by fastener, and second temperature control device is for controlling the temperature of chip.
Wafer support frame will not influence optical signal detecting.
Further, described testing agency's setting is in chip fixed mechanism side, and moveable in the y direction.Detection machine
Structure is driven by Y-direction driving mechanism, and detection can be scanned to chip different location.The Y-direction driving mechanism can be with
It is to drive testing agency to be moved by motor driven screw rod.
Further, the X-axis component drives Z axis component to be moved in X-direction by motor-driven mechanism or cylinder
It is dynamic.
Further, the Z axis component drives injection mechanism to be moved in Z-direction by motor-driven mechanism or cylinder
It is dynamic.
The beneficial effects of the present invention are:
Elisa assay instrument of the invention is the full-automatic analysis and testing equipment of high integration, there is automatic sampling to be loaded,
The functions such as automatic cleaning, temperature automatically controlled, automatic detection, can be realized high-throughput detection, and the optical signal inspection that the present invention designs
Mechanism is surveyed, it is sensitive can to improve detection using the straightline propagation of light and the principle of black material extinction, effective exclusive PCR light
Degree and detection accuracy.
Detailed description of the invention
Fig. 1 is elisa assay instrument complete machine schematic diagram of the present invention；
Fig. 2 is the structural schematic diagram of elisa assay instrument of the present invention testing agency；
Fig. 3 is the schematic illustration of elisa assay instrument of the present invention testing agency；
Fig. 4 is the structural schematic diagram of elisa assay instrument limiting device of the present invention；
Fig. 5 is the structural schematic diagram of elisa assay instrument chip fixed mechanism of the present invention；
Fig. 6 is the structural schematic diagram of elisa assay instrument injection mechanism of the present invention.
Specific embodiment
The present invention will be further explained below with reference to the attached drawings and specific examples, so that those skilled in the art can be with
It more fully understands the present invention and can be practiced, but illustrated embodiment is not as a limitation of the invention.
Embodiment 1:
In conjunction with attached drawing, a kind of elisa assay instrument of the invention, including rack 1；X-axis component 2, Z axis group are installed in rack
Part 3, reagent placement mechanism 4, wiper mechanism 5, chip fixed mechanism 6 and testing agency 7；
Z axis component 3 is fixedly mounted on X-axis component 2, can be driven by X-axis component 2 mobile in X-direction；The Z axis group
Injection mechanism 8 is installed, injection mechanism 8 can be driven lower movement in z-direction by Z axis component 3 on part 3；It can be by conjunction with Z axis component
X-axis Component driver is mobile in X-direction, and injection mechanism can be moved in X-direction and Z-direction；
Reagent placement mechanism 4, wiper mechanism 5 and chip fixed mechanism 6 are mounted on the lower section of injection mechanism 8, and reagent
Placement mechanism 4, wiper mechanism 5 and chip fixed mechanism 6 are arranged in X direction；Injection mechanism 8 can be in reagent placement mechanism 4, clear
It is moved between washing machine structure 5 and chip fixed mechanism 6, the operation such as is loaded, is sampled, cleaned；
Testing agency 7 is including laser emitter 71, optical signal detector 72 and is successively set on laser emitter 71 and light
The first loophole 73, filter 74 and the second loophole 75, the first loophole 73 and the second light transmission between signal detector 72
Hole 75 is coaxially disposed, and testing agency 7 is prepared using black material.Chip is placed in laser emitter 71 and the first loophole 73
Between detected.Laser emitter 71 selects the laser emitter of transmitting 450nm light wave, corresponding filter plate in the present embodiment
74 select the filter plate for penetrating 450nm light wave.Testing agency 7 of the invention further includes laser emitter limiting device 76, including
Third loophole 761 and laser emitter limit hole 762, third loophole 761 and the first loophole 73 are coaxially disposed, and diameter
Quite.The light that laser emitter 71 emits is projected by third loophole, through chip to be detected, thoroughly then in turn through first
Unthreaded hole 73, optical filter 74 and second penetrate hole 75, reach optical signal detector 72 and are detected.Specific reduction interference light
Schematic diagram is shown in Fig. 3, and according to the straightline propagation principle of light, the round of external disturbing rays closest to detector is along Fig. 3
Route 1 is propagated, and is then absorbed by black material, cannot be introduced into optical signal detector；In addition, formed by diffusing reflection one
A little light, the light only between route 2 and route 3 are possible to enter optical signal detector, most of also to cannot be introduced into
Optical signal detector, and the light between route 2 and route 3, there are also parts to be filtered out by filter plate, to the shadow of testing result
Sound is very small, and by above-mentioned design, influence of the extraneous light to the result of detection is reduced to minimum.
The setting of testing agency 7 of the invention is and moveable in the y direction in 6 side of chip fixed mechanism.Testing agency 7 is logical
It crosses Y-direction driving mechanism to be driven, detection can be scanned to chip different location.Y-direction driving mechanism, which can be, passes through motor
Screw rod is driven, testing agency is driven to be moved.
Reagent placement mechanism 4 of the invention includes the first temperature control equipment 41, reagent orifice plate 42 and is arranged in reagent wells
Lid 43 on plate 42；Lid 43 can be mobile in X-direction, convenient to protect to reagent.Reagent orifice plate 42 can be 96 orifice plates,
First temperature control equipment can be realized 2 DEG C~25 DEG C of accurate control, realize the further protection to reagent.
Wiper mechanism 5 of the invention includes rinse bath and spray equipment.Cleaning solution is pumped into spray equipment, passes through part
The mode of high pressure cleaning showers outside injection mechanism to cleaning, while injection mechanism is by drawing cleaning solution repeatedly, to note
The inside for penetrating mechanism is cleaned, and cleaning effect is more preferable, will not be impacted to reagent and chip to be detected.
Chip fixed mechanism 6 of the invention includes that (setting exists for wafer support frame 61, fastener 62 and second temperature control device
It on the inside of bracket, is not shown in figure).ELISA chip is fixed on wafer support frame 61 by fastener 62, second temperature control device
The temperature of 63 control chips, settable incubation temperature.Wafer support frame will not influence optical signal detecting.
Embodiment described above is only to absolutely prove preferred embodiment that is of the invention and being lifted, protection model of the invention
It encloses without being limited thereto.Those skilled in the art's made equivalent substitute or transformation on the basis of the present invention, in the present invention
Protection scope within.Protection scope of the present invention is subject to claims.
Claims (9)
1. a kind of elisa assay instrument, including rack；It is characterized in that,
X-axis component, Z axis component, reagent placement mechanism, wiper mechanism, chip fixed mechanism and detection are installed in the rack
Mechanism；
The Z axis component is fixedly mounted on X-axis component, can be mobile in X-direction by X-axis Component driver；The Z axis component
On injection mechanism is installed, the injection mechanism can be by the lower movement in z-direction of Z axis Component driver；
Reagent placement mechanism, wiper mechanism and the chip fixed mechanism is mounted on the lower section of injection mechanism, and reagent is put
Mechanism, wiper mechanism and chip fixed mechanism is set to arrange in X direction；
The testing agency includes laser emitter, optical signal detector and is successively set on laser emitter and optical signal and examines
The first loophole, filter and the second loophole between device are surveyed, first loophole and the second loophole are coaxially disposed,
The testing agency is prepared using black material.
2. a kind of elisa assay instrument according to claim 1, which is characterized in that the filter is to send out through laser
The filter of emitter launch wavelength.
3. a kind of elisa assay instrument according to claim 1, which is characterized in that the testing agency further includes laser
Transmitter limiting device, the laser emitter limiting device include third loophole and laser emitter limit hole, described
Third loophole and the first loophole be coaxially disposed.
4. a kind of elisa assay instrument according to claim 1, which is characterized in that the reagent placement mechanism includes the
One temperature control equipment, orifice plate and the lid being arranged on reagent orifice plate；The lid can be mobile in X-direction.
5. a kind of elisa assay instrument according to claim 1, which is characterized in that the wiper mechanism includes rinse bath
And spray equipment.
6. a kind of elisa assay instrument according to claim 1, which is characterized in that the chip fixed mechanism includes core
Piece support frame, fastener and second temperature control device.
7. a kind of elisa assay instrument according to claim 1, which is characterized in that the testing agency is arranged in chip
Fixed mechanism side, and it is moveable in the y direction.
8. a kind of elisa assay instrument according to claim 1, which is characterized in that the X-axis component passes through motor driven
Mechanism or cylinder driving Z axis component are moved in X-direction.
9. a kind of elisa assay instrument according to claim 1, which is characterized in that the Z axis component passes through motor driven
Mechanism or cylinder driving injection mechanism are moved in Z-direction.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811637624.9A CN109520930A (en) | 2018-12-29 | 2018-12-29 | A kind of elisa assay instrument |
| PCT/CN2019/116225 WO2020134630A1 (en) | 2018-12-29 | 2019-11-07 | Elisa analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811637624.9A CN109520930A (en) | 2018-12-29 | 2018-12-29 | A kind of elisa assay instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109520930A true CN109520930A (en) | 2019-03-26 |
Family
ID=65797372
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811637624.9A Pending CN109520930A (en) | 2018-12-29 | 2018-12-29 | A kind of elisa assay instrument |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN109520930A (en) |
| WO (1) | WO2020134630A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020134630A1 (en) * | 2018-12-29 | 2020-07-02 | 苏州汶颢微流控技术股份有限公司 | Elisa analyzer |
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| Publication number | Publication date |
|---|---|
| WO2020134630A1 (en) | 2020-07-02 |
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