CN108169501B - Magnetic enzyme fluorescent quantitative analyzer - Google Patents
Magnetic enzyme fluorescent quantitative analyzer Download PDFInfo
- Publication number
- CN108169501B CN108169501B CN201711256025.8A CN201711256025A CN108169501B CN 108169501 B CN108169501 B CN 108169501B CN 201711256025 A CN201711256025 A CN 201711256025A CN 108169501 B CN108169501 B CN 108169501B
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- quantitative analyzer
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- analyzer according
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- 102000004190 Enzymes Human genes 0.000 title claims description 6
- 108090000790 Enzymes Proteins 0.000 title claims description 6
- 238000001514 detection method Methods 0.000 claims abstract description 91
- 230000007246 mechanism Effects 0.000 claims abstract description 40
- 230000033001 locomotion Effects 0.000 claims abstract description 23
- 239000012085 test solution Substances 0.000 claims abstract description 15
- 239000007788 liquid Substances 0.000 claims description 26
- 239000000758 substrate Substances 0.000 claims description 13
- 230000005540 biological transmission Effects 0.000 claims description 10
- 238000010438 heat treatment Methods 0.000 claims description 10
- 229910052724 xenon Inorganic materials 0.000 claims description 7
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 claims description 7
- 238000010166 immunofluorescence Methods 0.000 claims 1
- 239000011324 bead Substances 0.000 abstract description 15
- 238000006243 chemical reaction Methods 0.000 abstract description 5
- 238000012360 testing method Methods 0.000 description 9
- 239000000243 solution Substances 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005213 imbibition Methods 0.000 description 3
- 238000009434 installation Methods 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00465—Separating and mixing arrangements
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a magneto-enzyme fluorescence-free quantitative analyzer, which comprises a bottom plate, wherein a plurality of pipetting mechanisms with the same structure are arranged on the bottom plate, each pipetting mechanism comprises a supporting frame, a front-back movement assembly is arranged on the supporting frame, a board clamp placing rack is arranged on the front-back movement assembly, a vertical movement assembly is arranged on the supporting frame, a mounting block is arranged on the vertical movement assembly, a pipetting piston is fixed on the mounting block, a detection driving assembly is arranged at the rear end of each pipetting mechanism, a detection mechanism is arranged on each detection driving assembly, the board clamp placing rack moves to a pipetting station, the pipetting piston places a sucked magnetic bead test solution into a test solution tank of a board clamp, and then the board clamp placing rack moves backwards to the side of the detection mechanism, so that the test solution tank is positioned below the detection mechanism, the detection is automatically completed by the detection mechanism, the whole reaction process is more intelligent, manual participation is reduced, and the detection precision and efficiency are improved.
Description
Technical Field
The invention relates to the field of chemical instruments and equipment, in particular to a magneto-enzyme fluorescent-free quantitative analyzer.
Background
The magnetoenzyme fluorescent quantitative analyzer is one for quantitatively detecting various analytes in serum, plasma or other body fluid with light emitting system and immunological analysis method. The reagent strip is filled with test solution and magnetic beads, the magnetic beads are magnetic solid-phase particles with certain granularity and are synthesized by using a high polymer material, the magnetic beads are used as various immunocompetent substances with specific affinity on a carrier coating, the various immunocompetent substances are sensitized to be immunomagnetic particles, the to-be-detected solution and the magnetic beads in the reagent strip are mixed and cultured, and then the to-be-detected solution is observed by using a detection system, so that a detection result is obtained. The common fluorescence reaction instrument on the market is to mix the magnetic bead test solution with the to-be-detected solution and then carry out color development observation or microscopic detection, and the to-be-detected solution and the magnetic bead test solution cannot be automatically mixed, and the preamble step still needs manual operation, so that the detection rate and the detection precision are very low.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention provides the magneto-enzyme fluorescent-free quantitative analyzer which can automatically mix the liquid to be detected and the magnetic bead test liquid, automatically detect and observe the mixed solution and greatly improve the processing efficiency and the accuracy.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
the utility model provides a magnetoenzyme exempts from fluorescence quantitative analysis appearance, includes the bottom plate, a plurality of pipetting devices that the structure is the same have been arranged on the bottom plate, pipetting devices includes the supporting rack, be provided with back-and-forth movement subassembly on the supporting rack, be provided with the integrated circuit board rack on the back-and-forth movement subassembly, be provided with the up-and-down movement subassembly on the supporting rack, set up the installation piece on the up-and-down movement subassembly, be fixed with the imbibition piston on the installation piece, the lower extreme of imbibition piston is provided with the suction head seat, be provided with the suction head of being connected with the imbibition piston in the suction head seat, pipetting devices's rear end is provided with detects drive assembly, be provided with detection mechanism on the detection drive assembly.
As a further improvement of the scheme, a rotating motor is arranged on the supporting frame, a main shaft of the rotating motor is hinged with a connecting rod through a transmission mechanism, the tail end of the connecting rod is provided with a magnet, and the rotating motor drives the connecting rod to rotate around a hinge point, so that the magnet is far away from or close to the board clamping rack.
As a further improvement of the scheme, the transmission mechanism is a gear set, and the gear set comprises a first gear sleeved on the rotating motor, a second gear hinged with the connecting rod, and a transmission gear connected with the first gear and the second gear.
As a further improvement of the scheme, the up-and-down motion assembly comprises a vertical sliding rail, a vertical sliding block is arranged on the vertical sliding rail, and the installation block is fixed on the vertical sliding block and is connected with a first driving motor.
As a further improvement of the scheme, the front-back movement assembly comprises a horizontal sliding rail, a horizontal sliding block is arranged on the horizontal sliding rail, the board card placing frame is fixed on the horizontal sliding block, and the board card placing frame is connected with a second driving motor.
As a further improvement of the scheme, the detection mechanism moves the mounting seat arranged on the detection driving assembly, a detection hole is formed in the mounting seat, a light emission channel is connected below the detection hole, a xenon light for emitting light is arranged in the light emission channel, a photomultiplier for receiving light is arranged beside the detection hole, a detection lens assembly is arranged at the front end of the photomultiplier, a detection scanner is fixed above the mounting seat, and the detection scanner is positioned above the detection hole.
As a further improvement of the above, the detection lens assembly includes a lens base, a convex lens disposed in the lens base from front to back, a first filter, and an acquisition photodiode.
As a further improvement of the scheme, a light source detection channel is further connected below the detection hole, a second filter and a detection photodiode which are arranged front and back are arranged in the light source detection channel, and the light source detection channel is mutually perpendicular to the light emission channel.
As a further improvement of the scheme, a standard substrate fixing seat is fixed beside the pipetting mechanism, a standard substrate board card is arranged in the standard substrate fixing seat, and the standard substrate fixing seat and the board card placing frame are located at the same height.
As a further improvement of the scheme, the bottom plate is provided with the heating device and the temperature controller, the board card rack is internally provided with the board card heating sheet, and the suction head seat is internally provided with the test solution heating sheet.
The invention has the beneficial effects that:
according to the magnetic enzyme fluorescence-free quantitative analyzer, the plate card is placed on the plate card placing frame, the liquid suction piston starts to suck up the magnetic bead test liquid in the plate card, the plate card placing frame moves to the liquid transfer station, the liquid suction piston places the sucked magnetic bead test liquid into the test liquid groove of the plate card, and then the plate card placing frame moves backwards to the side of the detection mechanism, so that the test liquid groove is located below the detection mechanism, the detection mechanism is utilized to automatically complete the detection, the whole reaction process is more intelligent, the participation of manpower is reduced, and the detection precision and efficiency are improved.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings that are required to be used in the description of the embodiments will be briefly described below. It is evident that the drawings described are only some embodiments of the invention, but not all embodiments, and that a person skilled in the art can obtain other designs and drawings from these drawings without inventive effort:
FIG. 1 is a schematic diagram of a preferred embodiment of the present invention;
FIG. 2 is a schematic structural diagram of a preferred embodiment of the present invention;
FIG. 3 is a schematic view showing a pipetting mechanism and a detecting mechanism according to a preferred embodiment of the invention;
FIG. 4 is a schematic view of a pipetting mechanism according to a preferred embodiment of the invention;
FIG. 5 is a schematic view of a pipetting mechanism according to a preferred embodiment of the invention;
FIG. 6 is a schematic view of a pipetting mechanism according to a preferred embodiment of the invention;
FIG. 7 is a front view of the detection mechanism according to the preferred embodiment of the present invention;
FIG. 8 is a schematic diagram of a detecting mechanism according to a preferred embodiment of the present invention;
fig. 9 is a cross-sectional view taken along direction AA in fig. 7.
Detailed Description
The conception, specific structure, and technical effects produced by the present invention will be clearly and completely described below with reference to the embodiments and the drawings to fully understand the objects, features, and effects of the present invention. It is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments, and that other embodiments obtained by those skilled in the art without inventive effort are within the scope of the present invention based on the embodiments of the present invention.
Referring to fig. 1 to 9, a magnetoenzyme fluorescence-free quantitative analyzer comprises a bottom plate 1, a plurality of pipetting mechanisms 2 with the same structure are arranged on the bottom plate 1, each pipetting mechanism 2 comprises a supporting frame 21, a front-back movement assembly 8 is arranged on the supporting frame 21, a board clamping rack 22 is arranged on the front-back movement assembly 8, a vertical movement assembly is arranged on the supporting frame 21, a mounting block 23 is arranged on the vertical movement assembly 7, a pipetting piston 24 is fixed on the mounting block 23, a suction head seat 25 is arranged at the lower end of the pipetting piston 24, a suction head 26 connected with the pipetting piston 24 is arranged in the suction head seat 25, a detection driving assembly 9 is arranged at the rear end of each pipetting mechanism 2, and a detection mechanism 3 is arranged on the detection driving assembly 9.
Referring to fig. 3 to 6, the up-and-down movement assembly 7 includes a vertical sliding rail 71, a vertical sliding block is disposed on the vertical sliding rail 71, the mounting block 23 is fixed on the vertical sliding block, and the mounting block 23 is connected with a first driving motor 72. The back and forth movement assembly 8 comprises a horizontal sliding rail 81, a horizontal sliding block 82 is arranged on the horizontal sliding rail 81, the board card placing frame 22 is fixed on the horizontal sliding block 82, and the board card placing frame 22 is connected with a second driving motor 83.
The mounting block 23 moves up and down along the vertical sliding rail 71, and the mounting block 23 moves downwards during liquid suction, so that the suction head 26 in the suction head seat 25 stretches downwards into the test liquid groove of the board card, then the piston block of the liquid suction piston 24 moves upwards, and the suction head 26 pumps up the test liquid to complete liquid suction. After the liquid suction is finished, the mounting block 23 moves upwards, so that the board card can conveniently move backwards, the empty slot in the board card is positioned below the suction head 26, then the piston block of the liquid suction piston 24 moves downwards, the test liquid in the suction head 26 is discharged into the empty slot, the whole liquid suction and liquid discharge process is finished fully automatically, and the processing efficiency is greatly improved.
The support frame 21 is provided with a rotating motor 27, a main shaft of the rotating motor 27 is hinged with a connecting rod 28 through a transmission mechanism 6, the tail end of the connecting rod 28 is provided with a magnet 29, and the rotating motor 27 drives the connecting rod 28 to rotate around a hinge point, so that the magnet 29 is far away from or close to the board card placing frame 22. The transmission mechanism 6 is a gear set, and the gear set comprises a first gear 61 sleeved on the rotating motor 27, a second gear 62 hinged with the connecting rod 28, and a transmission gear 63 connecting the first gear 61 and the second gear 62.
When in liquid suction, the connecting rod 28 drives the magnet 29 to be close to the board rack 22, so that magnetic beads in the board move and gather, and the magnetic beads deposited in the board move and stir, so that the magnetic bead reagent is more uniform, and the suction head 26 can conveniently suck magnetic bead test liquid. When the sucked test solution is discharged into the detection groove of the board, the connecting rod 28 drives the magnet 29 to be far away from the board rack 22, so that the magnetic beads are reset, the structural design is ingenious, and the efficiency of liquid suction and liquid transfer is greatly improved.
Referring to fig. 7 to 9, the detection mechanism 3 moves a mounting seat 31 disposed on the detection driving assembly, a detection hole 32 is disposed on the mounting seat 31, a light emission channel 33 is connected below the detection hole 32, a xenon light 34 for emitting light is disposed in the light emission channel 33, a photomultiplier 35 for receiving light is disposed beside the detection hole 32, a detection lens assembly is disposed at the front end of the photomultiplier 35, and the detection lens assembly includes a lens base 310, a convex lens 311, a first filter 312 and an acquisition photodiode 313 disposed in the lens base 310 from front to back.
The detection scanner 36 is fixed above the mounting seat 31, the detection scanner 36 is located above the detection hole 32, the detection scanner 36 reads and scans the bar code on the board card, can distinguish the test solution information, and stores the test solution information together with the photoelectric signal for subsequent data processing.
The detection driving assembly 9 comprises a detection sliding rail 91, a detection sliding block 92 is arranged on the detection sliding rail 91, the detection sliding block 92 is connected with a linear driving device, the mounting seat 31 is fixed on the detection sliding block 92, and the linear driving device can select different linear movement mechanisms such as a linear motor, a screw rod nut, a driving belt wheel and the like.
The xenon light 34 emits xenon light into the detection groove of the detection board card, the xenon light is emitted from the detection groove, the convex lens 311 collects light, the first filter 312 filters the ambient light, the stray light is prevented from affecting the result, the detection precision is improved, and the collecting photodiode 313 collects the light signal of the light. The photomultiplier 35 converts the optical signal into an electrical signal for transmission, gives out the relative fluorescence intensity according to the intensity of the electrical signal, analyzes the concentration of PCT, and finally calculates the result to realize automatic analysis and detection of the test solution, thereby greatly improving the detection speed and accuracy.
The lower part of the detection hole 32 is also connected with a light source detection channel 37, a second filter 38 and a detection photodiode 39 which are arranged front and back are arranged in the light source detection channel 37, the light source detection channel 37 is mutually perpendicular to the light emission channel 33, the light source detection channel 37 is used for detecting the intensity of emitted light, the intensity of the emitted light is ensured to be consistent, the quality is maintained during light detection, and the detection precision is improved.
The standard substrate fixing seat 4 is fixed beside the pipetting mechanism 2, the standard substrate board card 5 is arranged in the standard substrate fixing seat 4, the standard substrate fixing seat 4 and the board card placing frame 22 are positioned at the same height, before detection starts, the detection mechanism 3 moves to the lower part of the standard substrate fixing seat 4 to play a role in comparing the initial position, so that the xenon lamp 34 and the photomultiplier 35 can emit and receive light rays, and a positioning and aligning effect is played.
Be provided with heating device 10 and temperature controller 11 on the bottom plate 1, heating device heats whole equipment is inside, be equipped with the integrated circuit board heating plate in the integrated circuit board rack 22, be provided with test solution heating plate 210 in the suction head seat 25 for the test solution is in a invariable temperature always in the course of working, can not cool off, utilizes temperature controller 11 to detect the inside temperature of equipment, guarantees that the temperature of reaction is in the ideal within range, can not be too high or too low for the reaction result of test solution is more reliable.
While the preferred embodiments of the present application have been illustrated and described, the present application is not limited to the embodiments, and various equivalent modifications and substitutions can be made by one skilled in the art without departing from the spirit of the present application, and these equivalent modifications and substitutions are intended to be included in the scope of the present application as defined in the appended claims.
Claims (8)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711256025.8A CN108169501B (en) | 2017-12-04 | 2017-12-04 | Magnetic enzyme fluorescent quantitative analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711256025.8A CN108169501B (en) | 2017-12-04 | 2017-12-04 | Magnetic enzyme fluorescent quantitative analyzer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108169501A CN108169501A (en) | 2018-06-15 |
| CN108169501B true CN108169501B (en) | 2025-01-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711256025.8A Active CN108169501B (en) | 2017-12-04 | 2017-12-04 | Magnetic enzyme fluorescent quantitative analyzer |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109520930A (en) * | 2018-12-29 | 2019-03-26 | 苏州汶颢微流控技术股份有限公司 | A kind of elisa assay instrument |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06313767A (en) * | 1993-04-28 | 1994-11-08 | Medeika Tec Kk | Inspection apparatus for blood and the like using magnetic bead |
| CN101802164A (en) * | 2007-07-13 | 2010-08-11 | 汉迪实验室公司 | Intergrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples |
| CN207601097U (en) * | 2017-12-04 | 2018-07-10 | 珠海迪尔生物工程有限公司 | A kind of magnetic enzyme exempts from quantitative fluorescence analysis instrument |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8703492B2 (en) * | 2007-04-06 | 2014-04-22 | Qiagen Gaithersburg, Inc. | Open platform hybrid manual-automated sample processing system |
| CN101482462B (en) * | 2009-02-18 | 2011-09-28 | 长春光机医疗仪器有限公司 | Nano magnetic bead and liquid separation device of full-automatic light-emitting instrument |
| CN102147406B (en) * | 2011-02-14 | 2013-08-28 | 四川迈克生物科技股份有限公司 | Fully automatic chemical luminescent immune analyzer |
| CN105940094B (en) * | 2014-06-17 | 2018-02-23 | 深圳迈瑞生物医疗电子股份有限公司 | Nucleic acid-extracting apparatus and its method of work |
| WO2015192328A1 (en) * | 2014-06-17 | 2015-12-23 | 深圳迈瑞生物医疗电子股份有限公司 | Extract isolation apparatus and method of operation thereof |
| CN105891525B (en) * | 2016-07-05 | 2018-08-21 | 深圳普门科技有限公司 | The detection device of fully automatic electric chemical illumination immunity analysis instrument |
| CN106018784B (en) * | 2016-07-05 | 2019-02-22 | 深圳普门科技股份有限公司 | Small electrical chemical illumination immunity analysis instrument and its analysis method |
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2017
- 2017-12-04 CN CN201711256025.8A patent/CN108169501B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06313767A (en) * | 1993-04-28 | 1994-11-08 | Medeika Tec Kk | Inspection apparatus for blood and the like using magnetic bead |
| CN101802164A (en) * | 2007-07-13 | 2010-08-11 | 汉迪实验室公司 | Intergrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples |
| CN207601097U (en) * | 2017-12-04 | 2018-07-10 | 珠海迪尔生物工程有限公司 | A kind of magnetic enzyme exempts from quantitative fluorescence analysis instrument |
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Address after: 519041 No. 19 Golden Coast Road, San Zao Town, Jin Wan District, Zhuhai, Guangdong Applicant after: Zhuhai deer Bioengineering Co.,Ltd. Address before: 519041 No. 19 Golden Coast Road, San Zao Town, Jin Wan District, Zhuhai, Guangdong Applicant before: ZHUHAI DL MEDICAL BIOTECH Co.,Ltd. |
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