CN108653415A - A kind of composition and preparation method thereof with sedative action - Google Patents

Abstract

The invention belongs to the field of health food research and development, and in particular relates to a composition with hypnotic effect and a preparation method thereof. The raw materials of the composition are 50%~60% freeze-dried powder of ganoderma lucidum water extract and 40%~50% freeze-dried powder of traditional Chinese medicine in terms of mass fraction; the freeze-dried powder of traditional Chinese medicine includes the following raw materials in parts by weight: oyster powder 8- 15 parts, Poria cocos 5-8 parts, Suanzaoren 5-12 parts, ginseng 3-8 parts, licorice 1-5 parts. The product of the invention can give full play to the characteristics of various traditional Chinese medicines, and endow it with the effects of nourishing blood and calming the nerves, concentrating qi and clearing the heart, and calming the nerves through the synergistic effect of the traditional Chinese medicines.

Description

Composition with calming effect and preparation method thereof

technical field

The invention belongs to the field of health food research and development, and in particular relates to a composition with hypnotic effect and a preparation method thereof.

Background technique

Sleep disorders not only have a great impact on people's work, life, and physical and mental health, but are also early risk factors for diseases such as diabetes and cardiovascular diseases. Therefore, the treatment of insomnia is one of the hot spots of modern medical research. Today, modern medicine mostly uses barbiturates to treat insomnia, but due to the many adverse reactions of these drugs, long-term use is prone to tolerance and dependence, and damages liver and kidney functions, so people began to seek from traditional Chinese medicine. breakthrough. Traditional Chinese medicine preparations focus on the comprehensive therapy of removing asthenic fire, soothing the liver and regulating qi, nourishing the liver and kidney, and regulating qi and blood, so that the internal ecology of the human body can reach a harmonious and balanced state and achieve the goal of radical cure. After thousands of years of clinical practice, It has unique insights into improving sleep function.

CN 201410329578.1 discloses a health care product for improving sleep, which includes jujube seed, poria cocos, licorice, medlar, lily, bellflower and amomum. The active components of the health food and their weight ratios are: 8-25 grams of Suanzaoren, 5-25 grams of Poria cocos, 5-18 grams of licorice, 5-20 grams of medlar, 8-25 grams of lily, 5-20 grams of bellflower grams, Amomum 2-15 grams. The present invention uses a variety of medicinal and edible Chinese herbal medicines to extract active ingredients, combines the characteristics of traditional Chinese herbal medicines with modern medical research, makes various ingredients rationally formulated and shared, and achieves the health care function of improving sleep through multiple channels and multiple levels . The three traditional Chinese medicines wolfberry, platycodon grandiflorum and amomum used in this formula have the main functions of "clearing heat and relieving cough, nourishing stomach and strengthening spleen, warming lung and nourishing kidney". The main effect of this compound is to improve sleep, or to nourish yin and nourish stomach Mainly, it is not clear yet.

CN201510257789.3 discloses a health food granule with the function of improving sleep. The health food is prepared from the following components: Acanthopanax extract, green tea extract, lily extract, valerian extract, Passion fruit powder. The health food of the present invention selects Acanthopanax, green tea, lily, valerian, passionflower and other medicinal and edible Chinese herbal medicines to extract active ingredients, and combines the characteristics of traditional Chinese herbal medicines with the research on the characteristics of traditional European herbal medicines to make the various ingredients reasonable. The combination of prescriptions and joint use has multiple effects of improving sleep and alleviating anxiety, and the health care function of improving sleep is achieved through multiple approaches and levels. The compatibility of traditional Chinese medicine pays attention to the monarch, minister and assistant. This compound chooses valerian and passionflower, two traditional European plants. It is not clear whether it can assist the efficacy of the monarch medicine; and although the two medicines are mild in nature, they have strong effects. Whether it is suitable is unclear.

The present invention utilizes the principle of drug compatibility of "lord, minister, assistant envoy", highlights the efficacy of the four traditional Chinese medicines "sedation and tranquilization" of ganoderma lucidum, oyster powder, poria cocos, jujube seed, and utilizes the characteristics of "clearing away heat and detoxification" of two traditional Chinese medicines of ginseng and licorice to promote The invention has the health care function of improving sleep.

Contents of the invention

The object of the present invention is to provide a composition with a hypnotic effect and a preparation method thereof for the deficiencies of the prior art. The product of the invention can give full play to the characteristics of various traditional Chinese medicines, and endow it with the effects of nourishing blood and calming the nerves, concentrating qi and clearing the heart, and calming the nerves through the synergistic effect of the traditional Chinese medicines.

In order to realize the above-mentioned purpose of the invention, the present invention adopts following technical scheme:

A composition with calming effect, the raw materials are calculated by mass fraction, which is made of 50%~60% freeze-dried powder of ganoderma lucidum water extract and 40%~50% freeze-dried powder of traditional Chinese medicine; wherein the freeze-dried powder of traditional Chinese medicine includes the following parts by weight Raw materials: 8-15 parts of oyster powder, 5-8 parts of poria cocos, 5-12 parts of jujube seed, 3-8 parts of ginseng, 1-5 parts of licorice.

Preferably, the freeze-dried powder of traditional Chinese medicine comprises the following raw materials in parts by weight: 12 parts of oyster powder, 8 parts of poria cocos, 10 parts of jujube seed, 5 parts of ginseng, and 3 parts of licorice.

The preparation method of the water extract of Ganoderma lucidum is as follows: pulverize the fruiting bodies of Ganoderma lucidum, pass through a 150-200 mesh sieve, add distilled water to the sieved Ganoderma lucidum powder with a material-liquid ratio of 1-2g:15-30mL, Heating in a water bath at around 95°C for 1.5~2 hours, and after filtration under reduced pressure, the first filtrate and the first filter residue were collected; the first filter residue was heated in a water bath and filtered under reduced pressure according to the above steps, and the second filtrate and filter residue were collected. The second filter residue; the second filter residue is heated in a water bath and filtered under reduced pressure according to the above steps, and the third filtrate and the third filter residue are collected. After the three filtrates are combined, concentrated and freeze-dried, Ganoderma lucidum water is obtained. Extract lyophilized powder.

The preparation method of the freeze-dried traditional Chinese medicine powder is as follows: oyster powder, poria cocos, wild jujube seed, ginseng, and licorice are placed in a casserole, and distilled water is added according to a material-to-liquid ratio of 1~2g:10~20mL, boiled for 1~1.5h, and Press and filter to obtain the first filtrate and filter residue; boil the first filter residue according to the above steps for 0.5~1h, filter under reduced pressure to obtain the second filtrate and filter residue; combine the two filtrates, concentrate, freeze-dry to make frozen dry powder.

According to the mass ratio, 50%-60% of the freeze-dried powder of water extract of ganoderma lucidum and 40%-50% of the freeze-dried powder of traditional Chinese medicine are uniformly mixed, sieved, and the composition with soothing effect is obtained.

The beneficial effects of the present invention are:

1) Ganoderma lucidum water extract mainly contains polysaccharides, nucleoside compounds, alkaloid compounds and other components, which are mixed with Poria cocos, oyster powder, jujube seed, ginseng and licorice to form a composition, so that it can act differently in the body. Targets, forming a multi-target mechanism, can improve the sleep quality of insomnia patients, and can relieve the clinical symptoms of insomnia patients;

2) The product of the present invention is simple in manufacturing process, low in cost, easy to popularize, easy to carry, and convenient to eat.

Detailed ways

Below in conjunction with examples, more fully prove the preparation method and effect of the tranquilizing composition of the present invention. The preparation method of the present invention is not limited to the following examples, any changes to the preparation method of the present invention will fall within the protection scope of the present invention.

Example 1

A preparation method of a composition having a calming effect, the specific steps are:

1) The preparation method of the water extract of Ganoderma lucidum is as follows: crush the fruiting bodies of Ganoderma lucidum, pass through a 150-mesh sieve, add distilled water to the sieved Ganoderma lucidum powder at a ratio of 1g:15mL, and heat in a water bath at 90°C for 1.5h , after decompression filtration, collect filtrate and filter residue for the first time for the first time; filter residue for the first time according to the above steps for water bath heating, filter under reduced pressure, collect filtrate and filter residue for the second time; filter residue for the second time Heating in a water bath and filtering under reduced pressure according to the above steps, collecting the third filtrate and filter residue, combining the three filtrates, concentrating and freeze-drying to obtain the freeze-dried powder of the water extract of Ganoderma lucidum;

2) The preparation method of the freeze-dried powder of traditional Chinese medicine is as follows: put 12g of oyster powder, 8g of poria cocos, 10g of wild jujube seed, 5g of ginseng, and 3g of licorice into a casserole, add distilled water according to the ratio of material to liquid 1g:10mL, and cook for 1.5h. Filtrate under reduced pressure to obtain the filtrate and residue for the first time; boil the residue for the first time according to the above steps for 0.5h, filter under reduced pressure to obtain the filtrate and residue for the second time; combine the filtrates twice, concentrate, and freeze-dry to obtain frozen dry powder;

3) Mix 60wt% lyophilized powder of water extract of Ganoderma lucidum and 40wt% lyophilized powder of traditional Chinese medicine evenly, and sieve to prepare the composition with calming effect.

Example 2

A preparation method of a composition having a calming effect, the specific steps are:

1) The preparation method of the water extract of Ganoderma lucidum is as follows: crush the fruiting bodies of Ganoderma lucidum, pass through a 150-mesh sieve, add distilled water to the sieved Ganoderma lucidum powder at a ratio of 1g:15mL, and heat in a water bath at 90°C for 1.5h , after decompression filtration, collect filtrate and filter residue for the first time for the first time; filter residue for the first time according to the above steps for water bath heating, filter under reduced pressure, collect filtrate and filter residue for the second time; filter residue for the second time Heating in a water bath and filtering under reduced pressure according to the above steps, collecting the third filtrate and filter residue, combining the three filtrates, concentrating and freeze-drying to obtain the freeze-dried powder of the water extract of Ganoderma lucidum;

2) The preparation method of the freeze-dried powder of traditional Chinese medicine is as follows: put 8 g of oyster powder, 5 g of poria cocos, 5 g of jujube seed, 5 g of ginseng, and 2 g of licorice into a casserole, add distilled water according to the ratio of material to liquid: 1 g: 10 mL, and cook for 1.5 h. Filtrate under reduced pressure to obtain the filtrate and residue for the first time; boil the residue for the first time according to the above steps for 0.5h, filter under reduced pressure to obtain the filtrate and residue for the second time; combine the filtrates twice, concentrate, and freeze-dry to obtain frozen dry powder;

3) Mix 60wt% lyophilized powder of water extract of Ganoderma lucidum and 40wt% lyophilized powder of traditional Chinese medicine evenly, and sieve to prepare the composition with calming effect.

Example 3

A preparation method of a composition having a calming effect, the specific steps are:

1) The preparation method of the water extract of Ganoderma lucidum is as follows: crush the fruiting bodies of Ganoderma lucidum, pass through a 150-mesh sieve, add distilled water to the sieved Ganoderma lucidum powder at a ratio of 1g:15mL, and heat in a water bath at 90°C for 1.5h , after decompression filtration, collect filtrate and filter residue for the first time for the first time; filter residue for the first time according to the above steps for water bath heating, filter under reduced pressure, collect filtrate and filter residue for the second time; filter residue for the second time Heating in a water bath and filtering under reduced pressure according to the above steps, collecting the third filtrate and filter residue, combining the three filtrates, concentrating and freeze-drying to obtain the freeze-dried powder of the water extract of Ganoderma lucidum;

2) The preparation method of the freeze-dried powder of traditional Chinese medicine is as follows: put 15g of oyster powder, 8g of poria cocos, 12g of wild jujube seed, 8g of ginseng, and 5g of licorice into a casserole, add distilled water according to the ratio of material to liquid 1g:10mL, cook for 1.5h, Filtrate under reduced pressure to obtain the filtrate and residue for the first time; boil the residue for the first time according to the above steps for 0.5h, filter under reduced pressure to obtain the filtrate and residue for the second time; combine the filtrates twice, concentrate, and freeze-dry to obtain frozen dry powder;

3. Mix 60wt% ganoderma lucidum water extract freeze-dried powder and 40wt% traditional Chinese medicine freeze-dried powder evenly, and sieve to prepare the composition with calming effect.

Comparative example 1

Crush the fruiting bodies of Ganoderma lucidum, pass through a 150-mesh sieve, add distilled water to the sieved Ganoderma lucidum powder at a ratio of 1g:15mL, heat in a water bath at 90°C for 1.5h, filter under reduced pressure, and collect the filtrate for the first time and the filtrate for the first time. The first filter residue; the first filter residue was heated in a water bath and filtered under reduced pressure according to the above steps, and the second filtrate and filter residue were collected; the second filter residue was heated in a water bath and filtered under reduced pressure according to the above steps to collect the second filter residue The filtrates and residues of the three times were combined, concentrated and freeze-dried to obtain the freeze-dried powder of the water extract of Ganoderma lucidum, which was used as drug 1.

Comparative example 2

1) Crush the fruiting bodies of Ganoderma lucidum, pass through a 150-mesh sieve, add distilled water to the sieved Ganoderma lucidum powder at a ratio of 1g:15mL, heat in a water bath at 90°C for 1.5h, filter under reduced pressure, and collect the first filtrate and the first filter residue; the first filter residue is then heated in a water bath and filtered under reduced pressure according to the above steps, and the second filtrate and filter residue are collected; the second filter residue is then heated in a water bath and filtered under reduced pressure according to the above steps, and collected Obtain the filtrate and filter residue for the third time, combine the filtrates for the three times, concentrate and freeze-dry to obtain the freeze-dried powder of Ganoderma lucidum water extract;

2) Put 10g of oyster powder, 6g of poria cocos, and 8g of jujube seed in a casserole, add distilled water according to the ratio of material to liquid 1g:10mL, cook for 1.5h, and filter under reduced pressure to obtain the first filtrate and filter residue; put the first filter residue Then boil for 0.5h according to the above steps, filter under reduced pressure to obtain the second filtrate and filter residue; combine the two filtrates, concentrate, and freeze-dry to make freeze-dried powder;

3) Mix 60wt% lyophilized powder of Ganoderma lucidum water extract and 40wt% lyophilized powder of traditional Chinese medicine evenly, sieve, and use the mixed powder as drug 2.

Comparative example 3

1) Crush the fruiting bodies of Ganoderma lucidum, pass through a 150-mesh sieve, add distilled water to the sieved Ganoderma lucidum powder at a ratio of 1g:15mL, heat in a water bath at 90°C for 1.5h, filter under reduced pressure, and collect the first filtrate and the first filter residue; the first filter residue is then heated in a water bath and filtered under reduced pressure according to the above steps, and the second filtrate and filter residue are collected; the second filter residue is then heated in a water bath and filtered under reduced pressure according to the above steps, and collected Obtain the filtrate and filter residue for the third time, combine the filtrates for the three times, concentrate and freeze-dry to obtain the freeze-dried powder of Ganoderma lucidum aqueous extract;

2) Put 12g of oyster powder and 8g of poria cocos in a casserole, add distilled water according to the material-to-liquid ratio of 1g:10mL, boil for 1.5h, and filter under reduced pressure to obtain the first filtrate and filter residue; follow the above steps for the first filter residue Boil for 0.5h, filter under reduced pressure to obtain the second filtrate and filter residue; combine the two filtrates, concentrate, freeze-dry to make freeze-dried powder;

3) Mix 60wt% lyophilized powder of ganoderma lucidum water extract and 40wt% lyophilized powder of traditional Chinese medicine evenly, sieve, and use the mixed powder as drug 3.

Comparative example 4

1) Crush the fruiting bodies of Ganoderma lucidum, pass through a 150-mesh sieve, add distilled water to the sieved Ganoderma lucidum powder at a ratio of 1g:15mL, heat in a water bath at 90°C for 1.5h, filter under reduced pressure, and collect the first filtrate and the first filter residue; the first filter residue is then heated in a water bath and filtered under reduced pressure according to the above steps, and the second filtrate and filter residue are collected; the second filter residue is then heated in a water bath and filtered under reduced pressure according to the above steps, and collected Obtain the filtrate and filter residue for the third time, combine the filtrates for the three times, concentrate and freeze-dry to obtain the freeze-dried powder of Ganoderma lucidum water extract;

2) Put 15g of oyster powder in a casserole, add distilled water according to the material-to-liquid ratio of 1g:10mL, cook for 1.5 hours, and filter under reduced pressure to obtain the first filtrate and filter residue; cook the first filter residue according to the above steps for 0.5 h, filter under reduced pressure to obtain the second filtrate and filter residue; combine the two filtrates, concentrate, freeze-dry to make lyophilized powder;

3) Mix 60wt% lyophilized powder of ganoderma lucidum water extract and 40wt% lyophilized powder of traditional Chinese medicine evenly, sieve, and use the mixed powder as drug 4.

Application Example 1

The positive effects of the present invention on improving sleep will be further described below through animal experiment data, using the composition prepared in Example 1 as a sample.

1. Experimental animals

ICR-grade male mice, weighing between 18 and 20 g, were provided by Fuzhou General Hospital of Nanjing Military Region. Mice were housed in individually ventilated positive and negative pressure animal cages, maintained at controlled temperature (20±2°C) and humidity (60±15%), and maintained a 12-h light/dark cycle. Food and water were freely available during the rearing period, and they were acclimatized 7 days before the formal experiment.

2. Drug management

The lyophilized powder prepared in Example 1 and the medicine prepared in Comparative Examples 1-4.

3. Animal administration

The mice were randomly divided into 6 groups according to body weight, namely: control group and drug group. The experimental drug group was given corresponding drugs by intragastric administration, and the control group was given distilled water by intragastric administration, with a volume of 0.2mL/10g body weight, once a day for 21 consecutive days.

4. Experimental method

Experiments using sodium pentobarbital to induce mice (refer to the "Implementation Manual of Health Food Inspection and Evaluation Technical Specifications (2003 Edition)"):

a. Subthreshold Dose Hypnosis Experiment of Pentobarbital Sodium in Mice

One hour after the last administration in each group, each mouse was intraperitoneally injected with pentobarbital sodium solution (30mg/kg) 0.1mg/10g body weight, and the righting reflex disappeared for 1min as the sleep index, and the mice were placed on a warm blanket for Slow down its tension, and record the sleep occurrence rate of the mice within 30 min.

b. The Experiment of Prolonging the Sleep Time of Mice Induced by Sodium Pentobarbital

One hour after the last administration in each group, each mouse was intraperitoneally injected with pentobarbital sodium solution (50 mg/kg) 0.1 mg/10 g body weight. The righting reflex disappears for 1 minute as the sleep index, and the mice are placed on a warm blanket to reduce their tension. The sleep time of the mice in each group is recorded, and the sleep time of the mice exceeding 120 minutes is calculated as 120 minutes.

c. Experiment on Sleep Latency of Barbital Sodium in Mice

One hour after the last administration, each mouse was intraperitoneally injected with barbiturate sodium solution (250mg/kg) 0.1mg/10g body weight. Taking the loss of righting reflex as an indicator, the mice were placed on a warm blanket to reduce their tension, and the time from administration to the loss of righting reflex of mice in each group was recorded, and the sleep time of mice exceeding 60 minutes was calculated as 60 minutes.

5. Results

a. Effects of drugs combined with pentobarbital sodium on the incidence of sleep in mice

Compared with the control group, Example 1 and Comparative Example 2 combined with pentobarbital sodium had extremely significant differences (P<0.01) in the incidence of sleep in the mice, and the incidence of sleep in the mice in Example 1 reached the highest value. As shown in Table 1.

Table 1 Effects of drugs combined with pentobarbital sodium on the incidence of sleep in mice

Note: Compared with the control group, ^* P <0.05, ^** P <0.01. Tables 1, 2, and 3 are as above.

b. Effects of Drugs on Prolonging the Sleep Time of Mice by Sodium Pentobarbital

Compared with the control group, Example 1 showed a very significant synergistic effect of sedation and hypnosis, and had a very significant difference on the sleep time of mice (P <0.01), while Comparative Example 2 and Comparative Example 3 could significantly prolong the sleep of mice time (P<0.05), as shown in Table 2.

Table 2 Effect of drugs on prolonging the sleep time of mice by pentobarbital sodium

c. Effects of Drugs on Sleep Latency and Sleep Duration in Mice Treated with Barbital Sodium

Compared with the control group, the effect of Example 1 combined with barbital sodium has a very significant effect on the sleep latency of mice (P<0.01), and Comparative Example 2 has a significant effect (P<0.05). as shown in Table 3.

Table 3 Effects of drugs on sleep latency of mice treated with barbital sodium

Animal experiments have proved that the traditional Chinese medicine composition of the tranquilizing composition can improve the sleep rate of mice, prolong sleep time and shorten sleep latency. Illustrate that this composition does have the effect of improving sleep.

In the present invention, it is mixed with poria cocos, oyster powder, jujube seed, ginseng and licorice to form a composition, so that it acts on different targets in the body to form a multi-target mechanism, which can improve the sleep quality of insomnia patients and relieve insomnia patients. clinical symptoms, and the production process of the product is simple, low in cost, easy to promote, easy to carry, and convenient to eat, which provides a new choice for health care products that can improve sleep.

The above descriptions are only preferred embodiments of the present invention, and all equivalent changes and modifications made according to the scope of the patent application of the present invention shall fall within the scope of the present invention.

Claims (5)

1. a kind of composition with sedative action, it is characterised in that：Raw material is that 50% ~ 60% ganoderma lucidum water carries as mass fraction Object freeze-dried powder and 40% ~ 50% Chinese medicine freeze-dried powder；The Chinese medicine freeze-dried powder includes following raw material：8-15 parts of ostreae testa pulverata, 5-8 parts of Poria cocos, 5-12 parts of spina date seed, 3-8 parts of ginseng, 1-5 parts of Radix Glycyrrhizae.

2. the composition according to claim 1 with sedative action, it is characterised in that：The Chinese medicine freeze-dried powder include with Lower raw material：12 parts of ostreae testa pulverata, 8 parts of Poria cocos, 10 parts of spina date seed, 5 parts of ginseng, 3 parts of Radix Glycyrrhizae.

3. the composition according to claim 1 with sedative action, it is characterised in that：The ganoderma lucidum water extract freeze-dried powder Preparation method be：Ganoderma lucidum fruitbody is crushed, 150 ~ 200 mesh sieve is crossed, into the glossy ganoderma powder after sieving with 1 ~ 2g of solid-liquid ratio:15 Distilled water is added in ~ 30mL, in 90 ~ 95 DEG C of 1.5 ~ 2h of heating water bath, after being filtered under diminished pressure, collects to obtain first time filtrate and filter for the first time Slag；First time filter residue is subjected to heating water bath according still further to above-mentioned steps, is filtered under diminished pressure, second of filtrate and second of filter are collected to obtain Slag；Second of filter residue is subjected to heating water bath according still further to above-mentioned steps, is filtered under diminished pressure, third time filtrate and third time filter are collected to obtain After merging filtrate three times, after concentrated, freeze-drying, ganoderma lucidum water extract freeze-dried powder is made in slag.

4. the composition according to claim 1 with sedative action, it is characterised in that：The preparation of the Chinese medicine freeze-dried powder Method is：Ostreae testa pulverata, Poria cocos, spina date seed, ginseng, Radix Glycyrrhizae are placed in marmite, by 1 ~ 2g of solid-liquid ratio:Distillation is added in 10 ~ 20mL Water cooks 1 ~ 1.5h, is filtered under diminished pressure, obtains first time filtrate and filter residue；First time filter residue is pressed to above-mentioned steps again and cooks 0.5 ~ 1h, It is filtered under diminished pressure, obtains second of filtrate and filter residue；Merge filtrate twice, concentration, Chinese medicine freeze-dried powder is made in freeze-drying.

5. a kind of method preparing composition according to any one of claims 1-4, it is characterised in that：Ganoderma lucidum water extract is frozen Dry powder and Chinese medicine freeze-dried powder mix in proportion, and sieving obtains the composition with sedative action.