CN108641983B - 一种多效粉状复合em菌剂及其活化培养与应用 - Google Patents
一种多效粉状复合em菌剂及其活化培养与应用 Download PDFInfo
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- CN108641983B CN108641983B CN201810478143.1A CN201810478143A CN108641983B CN 108641983 B CN108641983 B CN 108641983B CN 201810478143 A CN201810478143 A CN 201810478143A CN 108641983 B CN108641983 B CN 108641983B
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- 230000001580 bacterial effect Effects 0.000 title claims abstract description 30
- 239000002131 composite material Substances 0.000 title claims abstract description 19
- 239000003795 chemical substances by application Substances 0.000 title abstract description 8
- 230000004913 activation Effects 0.000 title description 3
- 239000000843 powder Substances 0.000 title 1
- 238000000855 fermentation Methods 0.000 claims abstract description 40
- 230000004151 fermentation Effects 0.000 claims abstract description 40
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 29
- 239000007787 solid Substances 0.000 claims abstract description 24
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 21
- 239000002054 inoculum Substances 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 8
- 239000002068 microbial inoculum Substances 0.000 claims description 8
- 239000002994 raw material Substances 0.000 claims description 8
- 150000001875 compounds Chemical class 0.000 claims description 5
- 239000007788 liquid Substances 0.000 abstract description 17
- 238000009395 breeding Methods 0.000 abstract description 11
- 230000001488 breeding effect Effects 0.000 abstract description 11
- 230000007613 environmental effect Effects 0.000 abstract description 8
- 244000005700 microbiome Species 0.000 abstract description 8
- 238000003756 stirring Methods 0.000 abstract description 2
- 238000004806 packaging method and process Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 12
- 241000894006 Bacteria Species 0.000 description 9
- 239000002689 soil Substances 0.000 description 9
- 238000004332 deodorization Methods 0.000 description 6
- 238000009360 aquaculture Methods 0.000 description 5
- 244000144974 aquaculture Species 0.000 description 5
- 240000007594 Oryza sativa Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000010813 municipal solid waste Substances 0.000 description 2
- 230000000243 photosynthetic effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 241000193749 Bacillus coagulans Species 0.000 description 1
- 241000194108 Bacillus licheniformis Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 241000186000 Bifidobacterium Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000235646 Cyberlindnera jadinii Species 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000194032 Enterococcus faecalis Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 240000001046 Lactobacillus acidophilus Species 0.000 description 1
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 description 1
- 240000006024 Lactobacillus plantarum Species 0.000 description 1
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 1
- 241000238553 Litopenaeus vannamei Species 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 241000192001 Pediococcus Species 0.000 description 1
- 241000191996 Pediococcus pentosaceus Species 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 229940054340 bacillus coagulans Drugs 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940032049 enterococcus faecalis Drugs 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000007366 host health Effects 0.000 description 1
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- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 229940039695 lactobacillus acidophilus Drugs 0.000 description 1
- 229940072205 lactobacillus plantarum Drugs 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
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- 239000005017 polysaccharide Substances 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 239000012265 solid product Substances 0.000 description 1
- 239000002910 solid waste Substances 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- Life Sciences & Earth Sciences (AREA)
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- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
- Fodder In General (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
Description
菌种名称 | 含量 |
凝结芽孢杆菌 | 0~1*10<sup>11</sup>cfu/g |
光合细菌 | 0~1*10<sup>11</sup>cfu/g |
枯草芽孢杆菌 | 0~1*10<sup>11</sup>cfu/g |
地衣芽孢杆菌 | 0~1*10<sup>11</sup>cfu/g |
植物乳杆菌 | 1*10<sup>5</sup>~1*10<sup>11</sup>cfu/g |
嗜酸乳杆菌 | 1*10<sup>5</sup>~1*10<sup>11</sup>cfu/g |
粪肠球菌 | 1*10<sup>5</sup>~1*10<sup>11</sup>cfu/g |
乳酸片球菌 | 1*10<sup>5</sup>~1*10<sup>11</sup>cfu/g |
布氏乳杆菌 | 1*10<sup>5</sup>~1*10<sup>11</sup>cfu/g |
酿酒酵母 | 1*10<sup>5</sup>~1*10<sup>11</sup>cfu/g |
啤酒酵母 | 1*10<sup>5</sup>~1*10<sup>11</sup>cfu/g |
产朊假丝酵母 | 1*10<sup>5</sup>~1*10<sup>11</sup>cfu/g |
戊糖片球菌 | 1*10<sup>5</sup>~1*10<sup>11</sup>cfu/g |
双歧杆菌 | 1*10<sup>5</sup>~1*10<sup>11</sup>cfu/g |
总菌数 | 1*10<sup>6</sup>~1*10<sup>11</sup>cfu/g。 |
氨氮(ppm) | 0天 | 2天 | 4天 |
试验 | 1.8 | 0.3 | 0.1 |
空白对照 | 1.8 | 1.8 | 1.6 |
可见度(cm) | 0天 | 2天 | 4天 |
试验 | 15 | 45 | 40 |
空白对照 | 15 | 16 | 15 |
亚硝酸盐(ppm) | 0天 | 2天 | 4天 |
试验 | 0.4 | 0.15 | 0.05 |
空白对照 | 0.4 | 0.4 | 0.5 |
pH值 | 0天 | 1天 | 2天 |
试验 | 9.0 | 8.2 | 8.1 |
空白对照 | 8.9 | 9.0 | 9.1 |
臭味 | 0天 | 1天 | 2天 |
试验 | 臭 | 明显减少 | 基本不臭 |
对照 | 臭 | 臭 | 臭 |
每日投喂量(公斤/亩) | 0天 | 30天 | 60天 | 90天 | 130天 |
试验 | 0 | 10 | 31 | 51 | 85 |
对照 | 0 | 18 | 34 | 47 | 45 |
饵料系数 | 0天 | 135天(养殖结束) |
试验 | -- | 1.07 |
对照 | -- | 1.41 |
养殖产量(公斤/亩) | 0天 | 135天(养殖结束) |
试验 | -- | 657 |
对照 | -- | 488 |
臭味 | 0小时 | 2小时 | 4小时 | 6小时 |
试验 | 臭 | 明显减少 | 基本不臭 | 基本不臭 |
对照 | 臭 | 臭 | 臭 | 臭 |
腹泻数 | 0天 | 30天 | 60天 | 90天 |
试验 | 0 | 2% | 3.5% | 3% |
对照 | 0 | 5% | 7% | 6% |
藻类丰度(个/L) | 0天 | 1天 | 2天 |
试验 | 4.1×10<sup>6</sup> | 6.9×10<sup>4</sup> | 3.2×10<sup>4</sup> |
对照 | 5.3×10<sup>6</sup> | 5.1×10<sup>6</sup> | 5.9×10<sup>6</sup> |
pH | 0天 | 30天 | 60天 | 90天 |
试验 | 6.8 | 6.6 | 6.5 | 6.3 |
对照 | 6.8 | 6.8 | 6.8 | 6.8 |
Claims (2)
Priority Applications (1)
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CN201810478143.1A CN108641983B (zh) | 2018-05-18 | 2018-05-18 | 一种多效粉状复合em菌剂及其活化培养与应用 |
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CN201810478143.1A CN108641983B (zh) | 2018-05-18 | 2018-05-18 | 一种多效粉状复合em菌剂及其活化培养与应用 |
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Publication Number | Publication Date |
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CN108641983A CN108641983A (zh) | 2018-10-12 |
CN108641983B true CN108641983B (zh) | 2020-09-22 |
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Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN109548962B (zh) * | 2018-12-18 | 2022-09-16 | 江苏大有生物科技发展有限公司 | 一种包含植物乳杆菌的复合微生物菌剂及其应用 |
CN110343629B (zh) * | 2018-12-18 | 2022-03-18 | 江南大学 | 一种包含红曲霉的微生物菌剂及其应用 |
CN110343628B (zh) * | 2018-12-18 | 2022-08-09 | 江南大学 | 一种包含乙醇假丝酵母的微生物菌剂及其应用 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103416269A (zh) * | 2012-05-25 | 2013-12-04 | 鹤山市雷迅新农机械科技有限公司 | 一种量子新能水稻高产优质种植方法 |
CN106244503A (zh) * | 2016-09-29 | 2016-12-21 | 厦门华厦学院 | 一种除臭用em菌液制备方法 |
CN107012105A (zh) * | 2016-07-11 | 2017-08-04 | 田荣国 | 复合菌剂、病死动物无害化处理发酵菌剂、复合微生物肥、固体培养基和液体培养基 |
CN107827661A (zh) * | 2017-11-28 | 2018-03-23 | 芜湖四高农业科技有限公司 | 一种提高黄桃产量的复合肥制备方法 |
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2018
- 2018-05-18 CN CN201810478143.1A patent/CN108641983B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103416269A (zh) * | 2012-05-25 | 2013-12-04 | 鹤山市雷迅新农机械科技有限公司 | 一种量子新能水稻高产优质种植方法 |
CN107012105A (zh) * | 2016-07-11 | 2017-08-04 | 田荣国 | 复合菌剂、病死动物无害化处理发酵菌剂、复合微生物肥、固体培养基和液体培养基 |
CN106244503A (zh) * | 2016-09-29 | 2016-12-21 | 厦门华厦学院 | 一种除臭用em菌液制备方法 |
CN107827661A (zh) * | 2017-11-28 | 2018-03-23 | 芜湖四高农业科技有限公司 | 一种提高黄桃产量的复合肥制备方法 |
Non-Patent Citations (1)
Title |
---|
the use of effective microorganisms (EM) in organic waste management;david g. freitag;《sustainable community development》;20000303;全文 * |
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Address after: Room 504, Building 4, Jiangnan University National University Science and Technology Park, 99 Jinxi Road, Wuxi City, Jiangsu Province, China 214125 Patentee after: Dayou Biotechnology Development (Wuxi) Co.,Ltd. Country or region after: China Patentee after: Jiangnan University Address before: Room 319, Building A1, 999 Gaolang East Road, Wuxi City, Jiangsu Province, China 214131 Patentee before: JIANGSU DAYOU BIOLOGICAL DEVELOPMENTAL Co.,Ltd. Country or region before: China Patentee before: Jiangnan University |