CN108548879A - A kind of automatic rapid detection equipment - Google Patents
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- 239000007788 liquid Substances 0.000 description 10
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- G01N30/02—Column chromatography
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Abstract
Description
技术领域technical field
本发明涉及液相色谱领域，特别涉及一种自动化快速检测设备。The invention relates to the field of liquid chromatography, in particular to an automatic rapid detection device.
背景技术Background technique
随着现代化学技术的发展，得益于新材料、微电子、微加工等现代技术的应用，使得分析化学，尤其是仪器分析化学得到了长足发展与进步。作为仪器分析化学的重要分支，高效液相色谱超强的分离、分析能力使其在石油化工、有机合成、生理生化、医药卫生乃至空间探索等诸多领域得到了广泛的应用，并成为一种极为重要和不可缺少的手段。With the development of modern chemical technology, thanks to the application of new materials, microelectronics, micromachining and other modern technologies, analytical chemistry, especially instrumental analytical chemistry, has made great progress and progress. As an important branch of instrumental analytical chemistry, the super strong separation and analysis capabilities of high performance liquid chromatography make it widely used in many fields such as petrochemical, organic synthesis, physiology and biochemistry, medicine and health, and space exploration, and has become an extremely important and indispensable means.
近年来，随着技术的进步与人力成本的提高，高效液相色谱的自动化程度也越来越高，自动化装置的出现大大的提高了液相色谱的持续工作能力，提高了工作效率，降低了仪器使用时的人力成本。In recent years, with the advancement of technology and the increase of labor costs, the degree of automation of high performance liquid chromatography has become higher and higher. The emergence of automated devices has greatly improved the continuous working ability of liquid chromatography, improved work efficiency, and reduced Labor cost when using the instrument.
虽然高效液相色谱分析的自动化程度已经较高，但随着研究范围的扩大，样品来源也趋于复杂，化学分析人员需要花费大量的精力对样品进行前处理，以得到适合于高效液相色谱分析的物质，且处理得到的物质也需要人工操作放入高效液相色谱中，导致全实验流程自动化程度不够，对操作人员要求较高等问题，很多基层实验室并不具备上述条件。Although the automation of HPLC analysis has been relatively high, with the expansion of the scope of research, the source of samples tends to be more complex, and chemical analysts need to spend a lot of energy on pretreatment of samples to obtain samples suitable for HPLC. The analyzed substances and the processed substances also need to be manually put into high-performance liquid chromatography, resulting in insufficient automation of the entire experimental process and high requirements for operators. Many grassroots laboratories do not have the above conditions.
现在需要一种能够解决上述问题的自动化快速检测设备。Need a kind of automatic rapid detection equipment that can solve the above problems now.
发明内容Contents of the invention
本发明是为了解决现有技术所存在的上述不足，提出一种集成度高，用户操作简单，自动化程度高，结果准确可靠的自动化快速检测设备。The purpose of the present invention is to solve the above-mentioned deficiencies in the prior art, and propose an automatic rapid detection device with high integration, simple user operation, high degree of automation, and accurate and reliable results.
本发明的技术解决方案是：一种自动化快速检测设备，其特征在于：所述的设备包括二位六通阀11，所述二位六通阀11通过管路与二位三通阀12相连，而二位三通阀则通过第一二位T型阀8与清洗溶剂瓶1相连，而第一二位T型阀8上还设置有注射器7，The technical solution of the present invention is: an automatic rapid detection equipment, characterized in that: the equipment includes a two-position six-way valve 11, and the two-position six-way valve 11 is connected with a two-position three-way valve 12 through a pipeline , and the two-position three-way valve is connected to the cleaning solvent bottle 1 through the first two-position T-shaped valve 8, and the first two-position T-shaped valve 8 is also provided with a syringe 7,
所述二位六通阀11还通过管路与二位十通阀13相连，在二位十通阀13上分别连接有第一富集净化柱14和第二富集净化柱15，同时二位十通阀13还通过管路与样品针16相连，而与所述样品针16还相配有清洗器18，The two-position six-way valve 11 is also connected to the two-position ten-way valve 13 through a pipeline, and the first enrichment purification column 14 and the second enrichment purification column 15 are respectively connected to the two-position ten-way valve 13, and the two The ten-way valve 13 is also connected to the sample needle 16 through a pipeline, and the sample needle 16 is also equipped with a cleaner 18,
所述二位六通阀11还通过管路与第二二位T型阀10相连，所述的设备还包括多个溶剂瓶，这些溶剂瓶通过分配比例电磁阀6与溶剂泵9相连，而容积泵9的出口端则通过管路与第二二位T型阀10的一个端口相连，The two-position six-way valve 11 is also connected to the second two-position T-type valve 10 through a pipeline, and the described equipment also includes a plurality of solvent bottles, and these solvent bottles are connected to the solvent pump 9 through the distribution proportional solenoid valve 6, and The outlet port of the displacement pump 9 is connected to a port of the second two-position T-type valve 10 through a pipeline,
所述二位十通阀13还通过管路与分析色谱柱21相连，分析色谱柱21则与混合反应器22相连，所述混合反应器22的入口端分别与第一溶剂输送泵19和第二溶剂输送泵20相连，而混合反应器22的出口端则与检测器23相连，检测器23的出口端则通过管路与废液容器24相连，The two-position ten-way valve 13 is also connected to the analytical chromatographic column 21 through a pipeline, and the analytical chromatographic column 21 is connected to the mixing reactor 22, and the inlet port of the mixing reactor 22 is connected to the first solvent delivery pump 19 and the second solvent delivery pump 19 respectively. The two solvent delivery pumps 20 are connected, and the outlet end of the mixing reactor 22 is connected with the detector 23, and the outlet end of the detector 23 is connected with the waste liquid container 24 through a pipeline,
所述的二位三通阀12还通过管路与废液容器24相连，The two-position three-way valve 12 is also connected to the waste liquid container 24 through a pipeline,
所述的设备还包括样品托盘17，所述样品托盘17上设置有多个样品瓶。The device also includes a sample tray 17 on which a plurality of sample vials are arranged.
本发明同现有技术相比，具有如下优点：Compared with the prior art, the present invention has the following advantages:
本种结构形式的自动化快速检测设备，其结构合理，设计巧妙，它最大的特点在于，利用独特的双富集净化切换设计与多个阀门的相互切换配合，可以在整个分析实验中将富集净化操作与进样操作相互拆分，在第一通道进样分析时，同时开展第二通道的富集净化，并在第二通道进样分析时，同时开展第一通道的富集净化，并以此类推；它独特的双富集净化切换技术，大大缩减了每份样品的分析周期，极大提高了仪器的使用效率，降低了分析样品的平均成本；它用一台仪器实现了多套处理与检测设备的功能，减少了仪器购置成本；用户仅需操作一台设备即可实现样品的富集净化、进样分离、混合反应、分析检测，极大简化了实验操作；可依据设定，将多个溶剂瓶中的溶剂任意种类、任意比例混合形成溶液，不但配比准确，且可依据实际样品情况进行适当的调整，随配随用，无溶剂浪费，降低了溶剂使用成本，减小了环境污染。因此可以说这种自动化快速检测设备具备了上述系列优点，特别适合在本领域中推广应用，其市场前景十分广阔。This type of automatic rapid detection equipment has a reasonable structure and ingenious design. Its biggest feature is that it can use the unique double-enrichment purification switching design and the mutual switching of multiple valves to combine the enrichment and purification during the entire analysis experiment. The purification operation and the sampling operation are separated from each other. When the first channel is injected and analyzed, the enrichment and purification of the second channel is carried out at the same time, and when the second channel is injected and analyzed, the enrichment and purification of the first channel is carried out at the same time, and And so on; its unique dual-enrichment purification switching technology greatly reduces the analysis cycle of each sample, greatly improves the use efficiency of the instrument, and reduces the average cost of analyzing samples; it uses one instrument to realize multiple sets of The functions of processing and testing equipment reduce the cost of instrument purchase; users only need to operate one piece of equipment to achieve sample enrichment and purification, sample separation, mixed reaction, analysis and detection, which greatly simplifies the experimental operation; , mix any kind of solvents in multiple solvent bottles in any proportion to form a solution, not only the ratio is accurate, but also can be adjusted appropriately according to the actual sample situation, ready to use, no solvent waste, reducing the cost of solvent use, reducing Less environmental pollution. Therefore, it can be said that this automatic rapid detection equipment has the above-mentioned series of advantages, and is especially suitable for popularization and application in this field, and its market prospect is very broad.
说明书附图Instructions attached
图1为本发明实施例排气状态的结构示意图。Fig. 1 is a structural schematic diagram of an exhaust state according to an embodiment of the present invention.
图2为本发明实施例中第二富集净化柱清洗状态的结构示意图。Fig. 2 is a schematic structural view of the cleaning state of the second enrichment and purification column in the embodiment of the present invention.
图3为本发明实施例中第一富集净化柱清洗状态的结构示意图。Fig. 3 is a schematic structural view of the cleaning state of the first enrichment and purification column in the embodiment of the present invention.
图4为本发明实施例中第二富集净化柱活化状态的结构示意图。Fig. 4 is a schematic structural diagram of the activated state of the second enrichment and purification column in the embodiment of the present invention.
图5为本发明实施例中第一富集净化柱活化状态的结构示意图。Fig. 5 is a schematic structural diagram of the activated state of the first enrichment and purification column in the embodiment of the present invention.
图6为本发明实施例中第二富集净化柱上样、第一富集净化柱分析状态的结构示意图。Fig. 6 is a schematic structural view of the sample loading on the second enrichment and purification column and the analysis state of the first enrichment and purification column in the embodiment of the present invention.
图7为本发明实施例中第一富集净化柱上样、第二富集净化柱分析状态的结构示意图。Fig. 7 is a schematic structural diagram of the sample loading on the first enrichment and purification column and the analysis state of the second enrichment and purification column in the embodiment of the present invention.
具体实施方式Detailed ways
下面将结合附图说明本发明的具体实施方式。如图1至7所示：一种自动化快速检测设备，包括一个二位六通阀11，这个二位六通阀11通过管路与二位三通阀12相连，而二位三通阀则通过第一二位T型阀8与清洗溶剂瓶1相连，而第一二位T型阀8上还设置有注射器7，The specific implementation manner of the present invention will be described below with reference to the accompanying drawings. As shown in Figures 1 to 7: an automatic rapid detection device, including a two-position six-way valve 11, the two-position six-way valve 11 is connected to the two-position three-way valve 12 through a pipeline, and the two-position three-way valve is Connect to the cleaning solvent bottle 1 through the first two-position T-shaped valve 8, and the first two-position T-shaped valve 8 is also provided with a syringe 7,
上述的二位六通阀11还通过管路与二位十通阀13相连，在二位十通阀13上分别连接有第一富集净化柱14和第二富集净化柱15，同时二位十通阀13还通过管路与样品针16相连，而与所述样品针16还相配有清洗器18，The above-mentioned two-position six-way valve 11 is also connected to the two-position ten-way valve 13 through a pipeline, and the first enrichment purification column 14 and the second enrichment purification column 15 are respectively connected to the two-position ten-way valve 13, and the two The ten-way valve 13 is also connected to the sample needle 16 through a pipeline, and the sample needle 16 is also equipped with a cleaner 18,
二位六通阀11还通过管路与第二二位T型阀10相连，所述的设备还包括多个溶剂瓶，这些溶剂瓶通过分配比例电磁阀6与溶剂泵9相连，而容积泵9的出口端则通过管路与第二二位T型阀10的一个端口相连，The two-position six-way valve 11 is also connected to the second two-position T-type valve 10 through pipelines, and the described equipment also includes a plurality of solvent bottles, and these solvent bottles are connected to the solvent pump 9 through the distribution proportional electromagnetic valve 6, and the positive displacement pump The outlet port of 9 is connected to a port of the second two-position T-type valve 10 through a pipeline,
二位十通阀13还通过管路与分析色谱柱21相连，分析色谱柱21则与混合反应器22相连，所述混合反应器22的入口端分别与第一溶剂输送泵19和第二溶剂输送泵20相连，而混合反应器22的出口端则与检测器23相连，检测器23的出口端则通过管路与废液容器24相连，The two-position ten-way valve 13 is also connected to the analytical chromatographic column 21 through a pipeline, and the analytical chromatographic column 21 is then connected to the mixing reactor 22, and the inlet port of the mixing reactor 22 is connected with the first solvent delivery pump 19 and the second solvent respectively. The transfer pump 20 is connected, and the outlet end of the mixing reactor 22 is connected with the detector 23, and the outlet end of the detector 23 is connected with the waste liquid container 24 through a pipeline,
二位三通阀12还通过管路与废液容器24相连，The two-position three-way valve 12 is also connected to the waste liquid container 24 through a pipeline,
本设备还包括样品托盘17，所述样品托盘17上设置有多个样品瓶。The device also includes a sample tray 17 on which a plurality of sample vials are arranged.
本发明专利实施例的自动化快速检测设备的工作过程如下：本设备可依次实现排气步骤，第二富集净化柱15的清洗步骤，第一富集净化柱14的清洗步骤，第二富集净化柱15的活化步骤，第一富集净化柱14的活化步骤，第二富集净化柱15上样、第一富集净化柱14分析步骤，第一富集净化柱14上样、第二富集净化柱15分析步骤。The working process of the automatic rapid detection equipment of the patent embodiment of the present invention is as follows: the equipment can sequentially realize the exhaust step, the cleaning step of the second enrichment purification column 15, the cleaning step of the first enrichment purification column 14, the second enrichment purification The activation step of the purification column 15, the activation step of the first enrichment purification column 14, the loading of the second enrichment purification column 15, the analysis step of the first enrichment purification column 14, the loading of the first enrichment purification column 14, the second Enrichment purification column 15 analysis steps.
排气步骤（如图1所示）：在此步骤中，二位三通阀12切换至让第一二位T型阀8与排气管路相连的状态，暂时将清洗溶剂瓶1移开，首先利用注射器7将空气吸入到管路中（此时第一二位T型阀8与二位三通阀12相连的一侧关闭），然后再将空气通过二位三通阀12吹入到排气管路（此时第一二位T型阀8入气管路相连的一侧关闭），最终排放出去，该步骤能够将上一次工作后残留在管路中的气体排出。Exhaust step (as shown in Figure 1): In this step, the two-position three-way valve 12 is switched to the state where the first two-position T-valve 8 is connected to the exhaust pipeline, and the cleaning solvent bottle 1 is temporarily removed , first use the syringe 7 to suck air into the pipeline (at this time the side of the first two-position T-valve 8 connected to the two-position three-way valve 12 is closed), and then blow the air through the two-position three-way valve 12 To the exhaust pipeline (at this time, the side connected to the first and second T-shaped valve 8 gas inlet pipeline is closed), and finally discharged. This step can discharge the gas remaining in the pipeline after the last work.
第二富集净化柱15的清洗步骤（如图2所示）：在此步骤中，二位三通阀12、二位六通阀11和二位十通阀13分别作切换动作，重新构建本系统的管路连接方式，让清洗溶剂瓶1依次通过第一二位T型阀8、二位三通阀12、二位六通阀11、二位十通阀13和第二富集净化柱15与样品针16相连；利用注射器7将清洗溶剂瓶1中的清洗溶剂吸入到管路中（此时第一二位T型阀8与二位三通阀12相连的一侧关闭），然后再将清洗溶剂通过二位三通阀12依次送入二位六通阀11、二位十通阀13和第二富集净化柱15中（此时第一二位T型阀8入液管路相连的一侧关闭），清洗溶剂进入第二富集净化柱15后，对其进行清洗，清洗结束后的用过的清洗溶剂会进入到清洗器18中，完成第二富集净化柱15的清洗。Cleaning steps of the second enrichment and purification column 15 (as shown in Figure 2): In this step, the two-position three-way valve 12, the two-position six-way valve 11 and the two-position ten-way valve 13 perform switching actions respectively to rebuild The pipeline connection method of this system allows the cleaning solvent bottle 1 to pass through the first two-position T-shaped valve 8, the two-position three-way valve 12, the two-position six-way valve 11, the two-position ten-way valve 13 and the second enrichment purification The column 15 is connected to the sample needle 16; the cleaning solvent in the cleaning solvent bottle 1 is sucked into the pipeline by the syringe 7 (at this time, the side connecting the first two-position T-valve 8 and the two-position three-way valve 12 is closed), Then the cleaning solvent is sent into the two-position six-way valve 11, the two-position ten-way valve 13 and the second enrichment purification column 15 through the two-position three-way valve 12 (at this time, the first two-position T-type valve 8 enters the liquid The side connected to the pipeline is closed), after the cleaning solvent enters the second enrichment and purification column 15, it is cleaned, and the used cleaning solvent after cleaning will enter the cleaner 18 to complete the second enrichment and purification column 15 washes.
第一富集净化柱14的清洗步骤（如图3所示）：在此步骤中，二位三通阀12、二位六通阀11和二位十通阀13分别作切换动作，重新构建本系统的管路连接方式，让清洗溶剂瓶1依次通过第一二位T型阀8、二位三通阀12、二位六通阀11、二位十通阀13和第一富集净化柱14与样品针16相连；利用注射器7将清洗溶剂瓶1中的清洗溶剂吸入到管路中（此时第一二位T型阀8与二位三通阀12相连的一侧关闭），然后再将清洗溶剂通过二位三通阀12依次送入二位六通阀11、二位十通阀13和第一富集净化柱14中（此时第一二位T型阀8入液管路相连的一侧关闭），清洗溶剂进入第一富集净化柱14后，对其进行清洗，清洗结束后的用过的清洗溶剂会进入到清洗器18中，完成第一富集净化柱14的清洗。The cleaning step of the first enrichment and purification column 14 (as shown in Figure 3): In this step, the two-position three-way valve 12, the two-position six-way valve 11 and the two-position ten-way valve 13 perform switching actions respectively to rebuild The pipeline connection mode of this system allows the cleaning solvent bottle 1 to pass through the first two-position T-valve 8, the two-position three-way valve 12, the two-position six-way valve 11, the two-position ten-way valve 13 and the first enrichment purification The column 14 is connected to the sample needle 16; use the syringe 7 to suck the cleaning solvent in the cleaning solvent bottle 1 into the pipeline (at this time, the side where the first two-position T-valve 8 is connected to the two-position three-way valve 12 is closed), Then the cleaning solvent is sent into the two-position six-way valve 11, the two-position ten-way valve 13 and the first enrichment purification column 14 sequentially through the two-position three-way valve 12 (at this time, the first two-position T-type valve 8 enters the liquid The side connected to the pipeline is closed), after the cleaning solvent enters the first enrichment purification column 14, it is cleaned, and the used cleaning solvent after cleaning will enter the cleaner 18 to complete the first enrichment purification column 14 washes.
第二富集净化柱15的活化步骤（如图4所示）：在此步骤中，二位六通阀11和二位十通阀13分别作切换动作，重新构建本系统的管路连接方式，溶剂泵9、二位六通阀11、第二富集净化柱15与样品针16相连；利用分配比例电磁阀6从多个溶剂瓶中抽取溶剂，分配比例电磁阀6能够保证进入系统中各种溶剂之间的比例，混合溶剂在溶剂泵9的作用下通过二位六通阀11、二位十通阀13进入第二富集净化柱15，第二富集净化柱15活化后将混合溶剂直接通过样品针16排出到清洗器18中，完成第二富集净化柱15的活化步骤。The activation step of the second enrichment and purification column 15 (as shown in Figure 4): In this step, the two-position six-way valve 11 and the two-position ten-way valve 13 perform switching actions respectively to rebuild the pipeline connection mode of the system , the solvent pump 9, the two-position six-way valve 11, the second enrichment and purification column 15 are connected to the sample needle 16; the solvent is extracted from multiple solvent bottles by using the distribution ratio solenoid valve 6, and the distribution ratio solenoid valve 6 can ensure that it enters the system The ratio between various solvents, the mixed solvent enters the second enrichment and purification column 15 through the two-position six-way valve 11 and the two-position ten-way valve 13 under the action of the solvent pump 9, and the second enrichment and purification column 15 is activated. The mixed solvent is directly discharged into the washer 18 through the sample needle 16 to complete the activation step of the second enrichment and purification column 15 .
第一富集净化柱14的活化步骤（如图5所示）：在此步骤中，二位六通阀11和二位十通阀13分别作切换动作，重新构建本系统的管路连接方式，溶剂泵9、二位六通阀11、第一富集净化柱14与样品针16相连；利用分配比例电磁阀6从多个溶剂瓶中抽取溶剂，分配比例电磁阀6能够保证进入系统中各种溶剂之间的比例，混合溶剂在溶剂泵9的作用下通过二位六通阀11、二位十通阀13进入第一富集净化柱14，第一富集净化柱14活化后将混合溶剂直接通过样品针16排出到清洗器18中，完成第一富集净化柱14的活化步骤。The activation step of the first enrichment and purification column 14 (as shown in Figure 5): In this step, the two-position six-way valve 11 and the two-position ten-way valve 13 perform switching actions respectively to rebuild the pipeline connection mode of the system , the solvent pump 9, the two-position six-way valve 11, the first enrichment and purification column 14 are connected to the sample needle 16; the solvent is extracted from multiple solvent bottles by using the distribution ratio solenoid valve 6, and the distribution ratio solenoid valve 6 can ensure that it enters the system The ratio between various solvents, the mixed solvent enters the first enrichment and purification column 14 through the two-position six-way valve 11 and the two-position ten-way valve 13 under the action of the solvent pump 9, and the first enrichment and purification column 14 is activated. The mixed solvent is directly discharged into the washer 18 through the sample needle 16 to complete the activation step of the first enrichment and purification column 14 .
第二富集净化柱15上样（如图6所示）：在此步骤中，二位三通阀12、二位六通阀11和二位十通阀13分别作切换动作，重新构建本系统的管路连接方式，让第一二位T型阀8、二位三通阀12、二位六通阀11、二位十通阀13、第二富集净化柱15和样品针16相连，同时让溶剂泵9、二位六通阀11、二位十通阀13、第一富集净化柱14和分析色谱柱21相连；由注射器7提供动力，利用样品针16从样品托盘17上的某个样品瓶中抽取样品，样品进入第二富集净化柱15，实现第二富集净化柱15的上样步骤。Sample loading on the second enrichment and purification column 15 (as shown in Figure 6): In this step, the two-position three-way valve 12, the two-position six-way valve 11 and the two-position ten-way valve 13 perform switching actions respectively to rebuild the The pipeline connection method of the system connects the first two-position T-valve 8, the two-position three-way valve 12, the two-position six-way valve 11, the two-position ten-way valve 13, the second enrichment purification column 15 and the sample needle 16 At the same time, the solvent pump 9, the two-position six-way valve 11, the two-position ten-way valve 13, the first enrichment purification column 14 and the analysis chromatographic column 21 are connected; the power is provided by the syringe 7, and the sample needle 16 is used to remove the sample from the sample tray 17. A sample is extracted from a certain sample bottle, and the sample enters the second enrichment and purification column 15 to realize the sample loading step of the second enrichment and purification column 15 .
第一富集净化柱14上样、第二富集净化柱15分析步骤（如图7所示）：在此步骤中，二位三通阀12、二位六通阀11和二位十通阀13分别作切换动作，重新构建本系统的管路连接方式，让第一二位T型阀8、二位三通阀12、二位六通阀11、二位十通阀13、第一富集净化柱14和样品针16相连，同时让溶剂泵9、二位六通阀11、二位十通阀13、第二富集净化柱15和分析色谱柱21相连；由注射器7提供动力，利用样品针16从样品托盘17上的某个样品瓶中抽取样品，样品进入第一富集净化柱14中，实现第一富集净化柱14的上样步骤；The first enrichment purification column 14 loading, the second enrichment purification column 15 analysis steps (as shown in Figure 7): In this step, the two-position three-way valve 12, the two-position six-way valve 11 and the two-position ten-way The valves 13 perform switching actions respectively, and rebuild the pipeline connection mode of the system, so that the first two-position T-shaped valve 8, the two-position three-way valve 12, the two-position six-way valve 11, the two-position ten-way valve 13, the first The enrichment and purification column 14 is connected with the sample needle 16, and the solvent pump 9, the two-position six-way valve 11, the two-position ten-way valve 13, the second enrichment purification column 15 and the analysis chromatographic column 21 are connected; the power is provided by the syringe 7 , use the sample needle 16 to extract a sample from a certain sample bottle on the sample tray 17, and the sample enters the first enrichment purification column 14 to realize the sample loading step of the first enrichment purification column 14;
与此同时，利用分配比例电磁阀6从多个溶剂瓶中抽取溶剂，分配比例电磁阀6能够保证进入系统中各种溶剂之间的比例，混合溶剂在溶剂泵9的作用下通过二位六通阀11、二位十通阀13进入第二富集净化柱15，并连同之前已经位于第二富集净化柱15中的样品一同进入分析色谱柱21，并进入混合反应器22混合反应后，输送到专用检测器23检测分析；分析结束后，废弃的溶液直接排入废液容器24中；也就是说，本设备可同时实现第一富集净化柱14的上样和第二富集净化柱15的分析步骤。At the same time, use the distribution ratio solenoid valve 6 to extract solvents from multiple solvent bottles, the distribution ratio solenoid valve 6 can ensure the ratio between various solvents entering the system, and the mixed solvent passes through the two-position six The through valve 11 and the two-position ten-way valve 13 enter the second enrichment purification column 15, and together with the samples that have been located in the second enrichment purification column 15 before, enter the analytical chromatography column 21, and enter the mixing reactor 22 after the mixed reaction , transported to the special detector 23 for detection and analysis; after the analysis, the discarded solution is directly discharged into the waste liquid container 24; Analysis steps for purification column 15.
第二富集净化柱15上样、第一富集净化柱14分析步骤（如图6所示）：在此步骤中，二位三通阀12、二位六通阀11和二位十通阀13分别作切换动作，重新构建本系统的管路连接方式，让第一二位T型阀8、二位三通阀12、二位六通阀11、二位十通阀13、第二富集净化柱15和样品针16相连，同时让溶剂泵9、二位六通阀11、二位十通阀13、第一富集净化柱14和分析色谱柱21相连；由注射器7提供动力，利用样品针16从样品托盘17上的某个样品瓶中抽取样品，样品进入第二富集净化柱15，实现第二富集净化柱15的上样步骤；The second enrichment purification column 15 loading, the first enrichment purification column 14 analysis steps (as shown in Figure 6): In this step, the two-position three-way valve 12, the two-position six-way valve 11 and the two-position ten-way The valves 13 perform switching actions respectively, and rebuild the pipeline connection mode of this system, so that the first two-position T-shaped valve 8, the two-position three-way valve 12, the two-position six-way valve 11, the two-position ten-way valve 13, the second The enrichment and purification column 15 is connected with the sample needle 16, and at the same time, the solvent pump 9, the two-position six-way valve 11, the two-position ten-way valve 13, the first enrichment and purification column 14 are connected with the analysis chromatographic column 21; the power is provided by the syringe 7 , use the sample needle 16 to extract a sample from a certain sample bottle on the sample tray 17, and the sample enters the second enrichment purification column 15 to realize the sample loading step of the second enrichment purification column 15;
与此同时，利用分配比例电磁阀6从多个溶剂瓶中抽取溶剂，分配比例电磁阀6能够保证进入系统中各种溶剂之间的比例，混合溶剂在溶剂泵9的作用下通过二位六通阀11、二位十通阀13进入第一富集净化柱14，并连同之前已经位于第一富集净化柱14中的样品一同进入分析色谱柱21，并进入混合反应器22混合反应后，输送到专用检测器23检测分析；分析结束后，废弃的溶液直接排入废液容器24中；也就是说，本设备可同时实现第二富集净化柱15的上样和第一富集净化柱14的分析步骤。At the same time, use the distribution ratio solenoid valve 6 to extract solvents from multiple solvent bottles, the distribution ratio solenoid valve 6 can ensure the ratio between various solvents entering the system, and the mixed solvent passes through the two-position six The through valve 11 and the two-position ten-way valve 13 enter the first enrichment and purification column 14, and together with the samples that have been in the first enrichment and purification column 14 before, enter the analytical chromatographic column 21, and enter the mixing reactor 22 after the mixed reaction , transported to the special detector 23 for detection and analysis; after the analysis, the discarded solution is directly discharged into the waste liquid container 24; Analysis steps for purification column 14.
所有的分析都结束后，二位三通阀12、二位六通阀11和二位十通阀13分别作切换动作，重新构建本系统的管路连接方式，分别让本设备恢复到如图4和图5的状态，分别对第二富集净化柱和第一富集净化柱重新进行一次活化处理，最后将残留在本设备中的所有液态物质排出到废液容器24中即可。After all the analysis is over, the two-position three-way valve 12, the two-position six-way valve 11 and the two-position ten-way valve 13 respectively perform switching actions to rebuild the pipeline connection mode of the system and restore the equipment to the state shown in the figure. 4 and FIG. 5 , reactivate the second enrichment purification column and the first enrichment purification column respectively, and finally discharge all liquid substances remaining in the device into the waste liquid container 24 .
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