CN101620041B - Automatic flow-path separation device - Google Patents

Abstract

The invention relates to an automatic separation flow path device, which comprises a solvent bottle, an infusion pump, a six-way valve, a separation element, a collector, a sample bottle, a plunger pump and A waste liquid bottle is characterized in that: it comprises two three-way valves; the three ports of the first three-way valve are respectively connected to the solvent bottle, the six-way valve and the plunger pump through pipelines; the second The three ports of the three-way valve are respectively connected to the collector, the separation element and the waste liquid bottle through pipelines; the six-way valve is directly connected to the collector through a pipeline. Only two three-way valves are provided in the present invention. Compared with the existing separation flow device, the structure is simple and the cost is reduced. By controlling the switching between two three-way valves and one six-way valve, the plunger pump and sampling It also automatically completes the five processes of quantitative sampling, cleaning, sample separation, collection and discarding of samples. The invention improves the repetition accuracy, reduces the production cost of the instrument and the difficulty of daily maintenance.

Description

An automatic separation flow path device

technical field

The invention relates to a separation flow device used in the separation and preparation of biological or chemical samples, in particular to an automatic separation flow device.

Background technique

The existing common separation flow device (take the chromatograph as an example, as shown in Figure 1) mainly includes a solvent bottle 1, an infusion tube 2, an infusion pump 3, an infusion tube 4, a six-way valve 5, and an infusion tube 6 connected in sequence. , separation element 7 (chromatographic column), infusion tube 8, detector 9, infusion tube 10, collector 11, and sample bottle 12, etc. During the analysis process, sample injection and collection are generally cumbersome operations by hand, the analysis efficiency is low, and the repeatability is affected by the operator's proficiency. However, the existing automatic separation flow path device has a complex structure.

On October 25, 2005, the applicant proposed an invention patent named "an automatic separation flow path device" with the patent application number: 200510114554.5, which was improved on the basis of the existing manual operation ( As shown in Figure 2), it except comprises prior art solvent bottle 1, infusion tube 2, infusion pump 3, infusion tube 4, six-way valve 5, infusion tube 6, separating element 7 (chromatographic column), infusion tube 8 , detector 9, infusion tube 10, collector 11 and sample bottle 12, the main feature is to increase three three-way valves 21, 22, 23, a plunger pump 24, an electric control device, and collector 11 is both Sampling and collecting in one sample collector 11. The invention patent application can automatically complete the five processes of quantitative sampling, cleaning, sample separation, collection and discarding of samples, which overcomes the cumbersome manual operations in the prior art. However, because it includes three three-way valves 21, 22, and 23 respectively connected to the six-way valve 5 through pipelines, the manufacturing cost of the instrument is relatively high, the structure is relatively complicated, and maintenance is also difficult.

Contents of the invention

In view of the above problems, the purpose of the present invention is to provide an automatic separation flow channel device which can realize automatic flow control separation with simple structure and low cost.

In order to achieve the above, the present invention adopts the following technical solutions: an automatic separation flow path device, which includes a solvent bottle, an infusion pump, a six-way valve, a separation element, and a collector connected to each other through pipelines in sequence. A sample bottle, a plunger pump and a waste liquid bottle are characterized in that: it includes two three-way valves; the three ports of the first three-way valve are respectively connected to the solvent bottle and the six-way valve through pipelines and a plunger pump; the three ports of the second three-way valve are respectively connected to the collector, the separation element and the waste liquid bottle through pipelines; the six-way valve is directly connected to the collector through a pipeline.

A detector is connected on the pipeline between the separation element and the second three-way valve.

Because the present invention adopts the above technical scheme, it has the following advantages: 1. The present invention can overcome the prior art due to two three-way valves, a plunger pump and a motor controlled by a drive circuit in the separation flow device. tedious manual operation. 2. Since only two three-way valves are provided in the present invention, compared with the separation flow device with the patent No. 200510114554.5, the structure is simple and the cost is reduced. By controlling the switching between two three-way valves and one six-way valve, the The plunger pump and sampling collector are controlled, and the five processes of quantitative sampling, cleaning, sample separation, collection and discarding of samples are also automatically completed. The invention has a simple structure, improves the analysis efficiency and the repeatability of the analysis results, and reduces the production cost of the instrument and the difficulty of daily maintenance.

Description of drawings

Figure 1 is a schematic diagram of a separation flow path device operated manually

Figure 2 is a schematic diagram of a separation flow device with patent application number 200510114554.5

Fig. 3 is a schematic diagram of the separation flow path device of the present invention

Fig. 4 is a control circuit block diagram of the present invention

Fig. 5 is a schematic diagram of another separation channel device of the present invention

Detailed ways

The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

As shown in Figure 3, the present invention is an improvement on the basis of the existing manual operation flow path. In addition to including the solvent bottle 1, the pipeline 2, the infusion pump 3, the pipeline 4, and the six-way In addition to valve 5, pipeline 6, separation element 7, pipeline 8, detector 9, pipeline 10, collector 11, and sample bottle 12, the main features are that two three-way valves 13, 14 and a plunger pump are added 15. At the same time, the collector 11 is a collector 11 that combines sampling and collection. Wherein, the three ports of the first three-way valve 13 are respectively connected to the solvent bottle 1 by the pipeline 16, connected to the six-way valve 5 by the pipeline 17 and connected to the plunger pump 15 by the pipeline 18; the second three-way valve 14 The three ports are respectively connected to the detector 9 through the pipeline 10, connected to the sampling collector 11 through the pipeline 19, connected to the waste liquid bottle through the pipeline 20, and connected the six-way valve 5 to the collector 11 through the pipeline 21.

In the above-mentioned embodiment, the separation element 7 is a chromatographic column that can separate the liquid mixed sample, and can also be a gel column that can separate the liquid mixed sample, an ion exchange column, a column tube used in countercurrent separation, and a centrifugal partition chromatography. One of the distribution tanks, capillary tubes and solvent distillation columns used for capillary electrophoresis separation of samples.

In the foregoing embodiment, the separation element 7 is a chromatographic column for the separation of a solvent-soluble mixed sample, and may also be a gel column for the separation of a solvent-soluble mixed sample, an ion exchange column, a column tube for countercurrent separation, and a centrifuge. One of the distribution tanks used in partition chromatography, capillary tubes and solvent distillation columns used in capillary electrophoresis to separate samples.

As shown in Fig. 3 and Fig. 4, in the above-mentioned embodiment, each three-way valve 13, 14, six-way valve 5, and plunger pump 15 can be driven by controlling each motor M1, M2, M3, M4 through a drive circuit, such as a motor M1 drives the three-way valve 13 to connect with the solvent bottle 1, and the plunger pump 15 is connected to the six-way valve 5 to complete the quantitative extraction of the sample and the control of the solvent flow during sample injection and cleaning of the injection needle; the motor M2 drives the three-way valve 14 Control the solvent and sample flow during sample injection and separation and collection; the motor M3 drives the six-way valve 5 to control the components that transfer the external sample into the analysis flow path; the motor M4 drives the plunger pump 15 to cooperate with the three-way valves 13 and 14 to complete Quantitative extraction of solvents and samples. The solvent bottle 1 stores and provides the solvent as the mobile phase of the system. The inlet of the infusion pump 3 is connected to the solvent bottle 1 through the pipeline 2. The infusion pump 3 can always be turned on to extract the solvent and supply the flowing solvent with a constant flow rate throughout the pipeline. The separation element 7 is a component for separating samples; the detector 9 is a component for detecting samples. The collector 11 of the present invention combines sampling and collection, can form a sampling assembly with the sample bottle 12, and can form a collection assembly together with a collection bottle (not shown in the figure), and the collection bottle collects the separated sample components.

In the above-mentioned embodiment, according to the requirements of sample injection, elution analysis and collection of samples to be tested, it can be realized by starting different motors according to the sequence of flow path operation through general logic control and drive circuit, which is not a feature of the present invention. Herein No longer.

The dynamic process that the present invention needs to complete comprises: five dynamic processes (as shown in Figure 3) of system cleaning, sample extraction, sample separation, collection and discarding:

1. When the automatic separation flow path device is in the initial state, the direction of the solvent flow path is: solvent bottle 1-line 2-infusion pump 3-line 4-six-way valve 5-line 6-separation element 7-line 8 -Detector 9-pipeline 10-three-way valve 14-pipeline 20-waste liquid bottle, this is the cleaning state of the sample analysis pipeline.

2. When the automatic separation flow path device is in the state of extracting samples, the sampling collector 11 is inserted into the sample bottle 12, the motor M1 drives the three-way valve 13 to make the pipeline 17 communicate with the pipeline 18, and the motor M2 drives the three-way valve 14 to make the tube Lines 10 and 20 are connected, and the pipeline 19 is closed. The motor M3 drives the reversing rod in the six-way valve 5 to connect the pipelines 17 and 21. The motor M4 drives the plunger pump 15 to perform pulling action. The direction of the solvent flow path is: sample Bottle 12-sampling collector 11-pipeline 21-six-way valve 5-pipeline 17-three-way valve 13-pipeline 18-plunger pump 15, after one pull of the plunger pump 15, it can pass through the six-way The switching of the reversing lever in the valve 5 achieves the purpose of automatic quantitative sampling.

3. When the automatic separation flow device is in the state of washing the sampling pipeline, the sampling collector 11 moves out of the sample bottle 12, the three-way valve 14 does not move, and the motor M3 drives the six-way valve 5 to change direction so that the reversing rod inside is connected to the pipeline 4, 6, ready for sample separation, the motor M1 drives the three-way valve 13 to switch to connect the pipeline 16 and 18, the motor M4 drives the plunger pump 15 to perform the pulling action, and the solvent flow direction is: solvent bottle 1-pipeline 16 -Three-way valve 13-Pipeline 18-Plunger pump 15, the motor M1 drives the three-way valve 13 to switch to connect the pipelines 17 and 18, and the motor M4 drives the plunger pump 15 to perform push action, the direction of the solvent flow path is: plunger Pump 15-pipeline 18-three-way valve 13-pipeline 17-six-way valve 5-pipeline 21-sampling collector 11, discharge the waste liquid from the washing sampling pipeline into the waste liquid pool, and the plunger pump 15 can repeatedly Pull several times to clean the sampling line.

4. When the automatic separation flow path device is in the sample separation state, the direction of the solvent flow path is: solution bottle 1-line 2-infusion pump 3-line 4-six-way valve 5-line 6-separation element 7-line 8-detector 9-line 10-three-way valve 14-line 21-waste bottle. Observe the detector 9 during the elution process, and introduce unnecessary components into the waste liquid pool, which is discarded; once the peak value of the required components is found, the motor M2 drives the three-way valve 14 to switch to connect the pipelines 10 and 19, Then start to collect the detected sample components with the collection bottle.

5. After the first separated sample is collected, return to the initial state and clean the sample analysis pipeline. Then repeat the aforementioned separation process one by one for the remaining samples until all the samples are separated.

As shown in Figure 5, the present invention also provides a separation flow path device used under the condition that the separation time is determined. Compared with the above embodiment, the basic flow path is the same, the difference is that in this embodiment The flow path device does not include the detector 9, wherein the separation element 7 is directly connected to the three-way valve 14 through the pipeline 8.

Claims (2)

1. An automatic separation flow path device, which comprises a solvent bottle, an infusion pump, a six-way valve, a separation element, a collector, a sample bottle, a plunger pump and a waste liquid bottle, the solvent The bottle is sequentially connected to the infusion pump, the six-way valve and the inlet of the separation element through pipelines, which is characterized in that it also includes two three-way valves; the three ports of the first three-way valve are respectively connected to the The solvent bottle, the six-way valve and the plunger pump; the three ports of the second three-way valve are respectively connected to the inlet of the collector, the outlet of the separation element and the waste liquid bottle through pipelines; the six-way valve The collector is directly connected through a pipeline, and the collector combines sampling and collection together. 2 . The automatic separation flow device according to claim 1 , wherein a detector is connected to the pipeline between the separation element and the second three-way valve. 3 .

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