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CN107997002A - A kind of hypoglycemic compounding rice and its processing method - Google Patents

A kind of hypoglycemic compounding rice and its processing method Download PDF

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CN107997002A
CN107997002A CN201711085861.4A CN201711085861A CN107997002A CN 107997002 A CN107997002 A CN 107997002A CN 201711085861 A CN201711085861 A CN 201711085861A CN 107997002 A CN107997002 A CN 107997002A
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rice
hot pressing
hypoglycemic
compounding
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胡宏海
张泓
戴小枫
王娴
张春江
张良
刘倩楠
黄峰
田芳
张�荣
黄艳杰
徐芬
胡小佳
毕红霞
吴娱
谭瑶瑶
樊月
张娜娜
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Institute of Food Science and Technology of CAAS
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L7/00Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10Cereal-derived products
    • A23L7/143Cereal granules or flakes to be cooked and eaten hot, e.g. oatmeal; Reformed rice products
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L19/00Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/10Products from fruits or vegetables; Preparation or treatment thereof of tuberous or like starch containing root crops
    • A23L19/12Products from fruits or vegetables; Preparation or treatment thereof of tuberous or like starch containing root crops of potatoes
    • A23L19/15Unshaped dry products, e.g. powders, flakes, granules or agglomerates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/125Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

本发明属于食品领域,提出一种降血糖复配米,是由包括以下质量份数的原料经热压制成:基础复配粉100份,菊粉5~25份,多糖10~40份,燕麦麸15~50份,糊精5~20份,膳食纤维5~15份;其中所述基础复配粉由大米粉和马铃薯粉组成。本发明还提出所述降血糖复配米的加工方法。本发明的降血糖复配米,是以籼米‑马铃薯复配粉为主要原料,并添加一定比例的菊粉、大豆多糖、燕麦等辅料,采用双螺杆多效热压分子重构技术制得具有大米粒形状、质地和口感的降血糖复配米,为糖尿病患者提供一种可降低血糖的主食产品,为改善糖尿病人的日常饮食状况提供了一种有效的解决方案。The invention belongs to the field of food and proposes a hypoglycemic compound rice, which is made by hot pressing the following raw materials in parts by mass: 100 parts of basic compound powder, 5-25 parts of inulin, 10-40 parts of polysaccharide, 15-50 parts of oat bran, 5-20 parts of dextrin, 5-15 parts of dietary fiber; wherein the basic compound powder is composed of rice flour and potato flour. The invention also proposes a processing method of the hypoglycemic compound rice. The blood-sugar-lowering compound rice of the present invention is based on indica rice-potato compound powder as the main raw material, and a certain proportion of inulin, soybean polysaccharide, oat and other auxiliary materials are added, and the double-screw multi-effect hot-pressed molecular reconstruction technology is used to obtain The hypoglycemic compound rice with rice grain shape, texture and taste provides a staple food product that can lower blood sugar for diabetic patients, and provides an effective solution for improving the daily diet of diabetic patients.

Description

一种降血糖复配米及其加工方法A kind of hypoglycemic compound rice and its processing method

技术领域technical field

本发明属于食品领域,具体涉及一种降血糖的复配大米及其加工方法。The invention belongs to the field of food, and in particular relates to a compound rice for lowering blood sugar and a processing method thereof.

背景技术Background technique

我国糖尿病总体患病率已达9.7%,糖尿病只能进行药物和食物控制,而市场上可供糖尿病人群选用的主食产品极少,糖尿病治疗面临挑战。近年来,国内外在具有降血糖作用的天然产物上已有大量的药理和临床研究,发现马铃薯、菊粉、大豆多糖、燕麦麸等具有多种生物活性,有降血糖、降血脂、抗肿瘤和抗氧化功能。马铃薯多以鲜食菜用为主,而菊粉、大豆多糖多以胶馕、冲剂、片剂等药品形态食用。同时,燕麦麸因含有大量膳食纤维,口感较差,应用受限。目前,以马铃薯全粉、菊粉、大豆多糖、燕麦麸等为原辅料的具有食品形态的主食产品尚未见报道。因此,以籼米、马铃薯全粉、菊粉、大豆多糖、燕麦麸等为原辅料,利用双螺杆多效热压分子重构技术,使上述原辅料发生分子交联重组,制得具有大米粒形状、质地和口感的新型降糖主食产品,对于改善糖尿病人的日常饮食具有重要意义。The overall prevalence of diabetes in my country has reached 9.7%. Diabetes can only be controlled with drugs and food, but there are very few staple food products available for people with diabetes on the market. Diabetes treatment is facing challenges. In recent years, there have been a large number of pharmacological and clinical studies on natural products with hypoglycemic effects at home and abroad. It has been found that potatoes, inulin, soybean polysaccharides, and oat bran have various biological activities, such as hypoglycemic, blood lipid, and antitumor. and antioxidant function. Potatoes are mostly eaten fresh, while inulin and soybean polysaccharides are mostly eaten in the form of rubber naan, granules, tablets and other medicines. At the same time, because oat bran contains a lot of dietary fiber, its taste is poor, and its application is limited. At present, there are no reports on staple food products with food form using whole potato powder, inulin, soybean polysaccharide, oat bran, etc. as raw and auxiliary materials. Therefore, using indica rice, whole potato powder, inulin, soybean polysaccharide, oat bran, etc. The new hypoglycemic staple food product with quality, texture and taste is of great significance for improving the daily diet of diabetics.

发明内容Contents of the invention

针对本领域存在的不足之处,本发明的目的是提出一种降血糖复配米。本发明的目的还在于通过开发具有降糖功效的复配米产品,以扩大马铃薯、菊粉、大豆多糖、燕麦麸等的消费量。Aiming at the deficiencies in the art, the purpose of the present invention is to propose a hypoglycemic compound rice. The purpose of the present invention is also to expand the consumption of potatoes, inulin, soybean polysaccharides, oat bran, etc. by developing compound rice products with hypoglycemic effects.

本发明的第二个目的是提出所述降血糖复配米的加工方法。The second object of the present invention is to propose a processing method of the hypoglycemic compound rice.

实现本发明目的的技术方案为:The technical scheme that realizes the object of the present invention is:

一种降血糖复配米,是由包括以下质量份数的原料经热压制成:基础复配粉100份,菊粉5~25份,多糖10~40份,燕麦麸15~50份,糊精5~20份,膳食纤维5~15份;其中所述基础复配粉由大米粉和马铃薯粉组成。A hypoglycemic compound rice is prepared by hot pressing the following raw materials in parts by mass: 100 parts of basic compound powder, 5-25 parts of inulin, 10-40 parts of polysaccharide, 15-50 parts of oat bran, 5-20 parts of dextrin, 5-15 parts of dietary fiber; wherein the basic compound powder is composed of rice flour and potato flour.

其中,所述基础复配粉由大米粉45~65份和马铃薯粉35~55份组成,所述大米粉为籼米粉或粳米粉。Wherein, the basic compound flour is composed of 45-65 parts of rice flour and 35-55 parts of potato flour, and the rice flour is indica rice flour or japonica rice flour.

其中,所述多糖为大豆多糖、银耳多糖、海带多糖、人参多糖、红景天多糖中的一种或多种。Wherein, the polysaccharide is one or more of soybean polysaccharides, white fungus polysaccharides, laminarin polysaccharides, ginseng polysaccharides, and rhodiola polysaccharides.

优选地,所述膳食纤维为超细膳食纤维,粒度为150~250目。Preferably, the dietary fiber is ultrafine dietary fiber with a particle size of 150-250 mesh.

所述的降血糖复配米,优选地,是由包括以下质量份数的原料经热压制成:基础复配粉100份,菊粉15~25份,多糖15~25份,燕麦麸20~25份,糊精20份,膳食纤维10~15份。The hypoglycemic compound rice is preferably prepared by hot pressing the following raw materials in parts by mass: 100 parts of basic compound powder, 15-25 parts of inulin, 15-25 parts of polysaccharide, 20 parts of oat bran ~ 25 parts, dextrin 20 parts, dietary fiber 10-15 parts.

所述降血糖复配米的加工方法,包括步骤:The processing method of the described hypoglycemic compound rice comprises the steps of:

(1)双螺杆多效热压,工艺参数如下:螺杆转速80-130rpm,机筒温度依次为1效热压区50-70℃,2效热压区55-85℃,3效热压区60-90℃,4效热压区90-120℃,5效热压区65-90℃,6效热压区45-70℃;(1) Twin-screw multi-effect hot pressing, the process parameters are as follows: screw speed 80-130rpm, barrel temperature is 50-70°C for the first-effect hot-press zone, 55-85°C for the second-effect hot-press zone, and 3-effect hot-press zone 60-90℃, 4-effect hot-press zone 90-120℃, 5-effect hot-press zone 65-90℃, 6-effect hot-press zone 45-70℃;

(2)老化和干燥:热压得到的物料经过老化工艺,老化的温度3-10℃,时间6-12小时;然后干燥,干燥的温度为30~60℃,干燥时间10~15小时。(2) Aging and drying: The material obtained by hot pressing is subjected to an aging process at a temperature of 3-10° C. for 6-12 hours; then dried at a temperature of 30-60° C. and a drying time of 10-15 hours.

其中,步骤(1)双螺杆多效热压时的喂料转速为25-65rpm,液体泵转速为20-55rpm,切粒转速为45-80rpm。Wherein, in the step (1) twin-screw multi-effect hot pressing, the feeding speed is 25-65rpm, the liquid pump speed is 20-55rpm, and the cutting speed is 45-80rpm.

进一步地,步骤(1)双螺杆多效热压后,将物料经切粒装置切割为大米粒形状。Further, after step (1) twin-screw multi-effect hot pressing, the material is cut into the shape of rice grains by a cutting device.

优选地,步骤(2)所述的干燥为三段干燥,其中一段干燥的温度为50℃,湿度80%,时间1小时;二段干燥的温度为55℃,湿度85%,时间2小时;三段干燥的温度为35℃,湿度70%,时间10小时。Preferably, the drying described in step (2) is three-stage drying, wherein the temperature of the first-stage drying is 50°C, the humidity is 80%, and the time is 1 hour; the temperature of the second-stage drying is 55°C, the humidity is 85%, and the time is 2 hours; The temperature of the three-stage drying is 35° C., the humidity is 70%, and the time is 10 hours.

本发明的有益效果在于:The beneficial effects of the present invention are:

本发明的降血糖复配米,是以籼米-马铃薯复配粉为主要原料,并添加一定比例的菊粉、大豆多糖、燕麦等辅料,采用双螺杆多效热压分子重构技术制得具有大米粒形状、质地和口感的降血糖复配米,为糖尿病患者提供一种可降低血糖的主食产品,为改善糖尿病人的日常饮食状况提供了一种有效的解决方案。The hypoglycemic compound rice of the present invention uses indica rice-potato compound powder as the main raw material, adds a certain proportion of inulin, soybean polysaccharide, oat and other auxiliary materials, and adopts twin-screw multi-effect hot pressing molecular reconstruction technology to obtain The hypoglycemic compound rice with rice grain shape, texture and taste provides a staple food product that can lower blood sugar for diabetic patients, and provides an effective solution for improving the daily diet of diabetic patients.

本发明的降糖复配米蒸煮损失为11.6-14.5%,体外预测血糖生成指数为38.1-50.4,属于低血糖生成指数食物。The cooking loss of the hypoglycemic compound rice of the invention is 11.6-14.5%, and the predicted glycemic index in vitro is 38.1-50.4, which belongs to low glycemic index food.

本发明的降糖复配米对2型糖尿病模型Wister大鼠有显著的降血糖效果,食用本发明的降糖复配米35天后,2型糖尿病模型Wister大鼠的血糖值由26.1mmol/L降低至13.6mmol/L。The hypoglycemic compound rice of the present invention has a significant hypoglycemic effect on type 2 diabetes model Wister rats, and after eating the hypoglycemic compound rice of the present invention for 35 days, the blood sugar level of type 2 diabetes model Wister rats is increased by 26.1mmol/L Reduced to 13.6mmol/L.

附图说明Description of drawings

图1为降血糖复配米加工工艺流程图。Figure 1 is a flow chart of the processing technology for hypoglycemic compound rice.

图2为降糖复配米对2型糖尿病模型Wister大鼠的降血糖效果比较图。Figure 2 is a comparison chart of the hypoglycemic effect of the hypoglycemic compound rice on type 2 diabetes model Wister rats.

具体实施方式Detailed ways

现以以下实施例来说明本发明,但不用来限制本发明的范围。实施例中使用的手段,如无特别说明,均使用本领域常规的手段。The present invention is now illustrated with the following examples, which are not intended to limit the scope of the present invention. The means used in the examples, unless otherwise specified, are conventional means in the art.

下述实施例中所使用的实验方法如无特殊说明,均为常规方法。The experimental methods used in the following examples are conventional methods unless otherwise specified.

下述实施例中所用的材料、试剂等,如无特殊说明,均可从商业途径得到。The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

实施例1:Example 1:

本实施例的降糖复配米由下述重量份的原料制得:基础复配粉(籼米粉55,马铃薯粉45),菊粉15,大豆多糖15,燕麦麸20,糊精20,超细膳食纤维15(200目)。The hypoglycemic compound rice of the present embodiment is made from the following raw materials by weight: basic compound powder (indica rice flour 55, potato flour 45), inulin 15, soybean polysaccharide 15, oat bran 20, dextrin 20, super Fine dietary fiber 15 (200 mesh).

本发明的降糖复配米加工工艺见图1,包括步骤,The hypoglycemic compound rice processing technology of the present invention is shown in Fig. 1, comprises steps,

1)混匀:将上述原料投入混料机混合均匀1) Mixing: Put the above raw materials into the mixer and mix evenly

2)双螺杆多效热压工艺参数如下:螺杆转速120rpm,机筒温度依次为1效热压区60℃,2效热压区55℃,3效热压区80℃,4效热压区120℃,5效热压区90℃,6效热压区70℃;喂料转速为65rpm,液体泵转速为55rpm;切粒转速为70rpm;2) The parameters of the twin-screw multi-effect hot pressing process are as follows: screw speed 120rpm, the barrel temperature is 60°C for the first effect hot pressing zone, 55°C for the second effect hot pressing zone, 80°C for the third effect hot pressing zone, and 80°C for the fourth effect hot pressing zone 120°C, 5-effect hot-press zone 90°C, 6-effect hot-press zone 70°C; feeding speed is 65rpm, liquid pump speed is 55rpm; pelletizing speed is 70rpm;

3)切粒/成型:多效热压分子重构后的物料经切粒成型装置切割成大米粒形状的复配米。3) Pelletizing/shaping: The material after molecular reconstruction by multi-effect hot pressing is cut into compound rice in the shape of rice grains by a pelletizing and forming device.

4)老化和干燥:老化工艺温度5±2℃,时间10小时;4) Aging and drying: Aging process temperature 5±2℃, time 10 hours;

5)干燥工艺一段干燥:温度为50℃,湿度80%,时间1小时;二段干燥:55℃,湿度85%,时间2小时;三段干燥:35℃,湿度70%,时间10小时。5) Drying process One-stage drying: temperature 50°C, humidity 80%, time 1 hour; second-stage drying: 55°C, humidity 85%, time 2 hours; third-stage drying: 35°C, humidity 70%, time 10 hours.

6)成品:将干燥后的复配米冷却至室温,制得复配米成品。6) Finished product: the dried compounded rice is cooled to room temperature to obtain a compounded rice product.

实施例2、Embodiment 2,

本实施例的降糖复配米由下述重量份的原料制得:基础复配粉(籼米粉60,马铃薯粉40),菊粉15,大豆多糖20,燕麦麸25,糊精10,超细膳食纤维10。The hypoglycemic compound rice of the present embodiment is made from the following raw materials by weight: basic compound powder (indica rice flour 60, potato flour 40), inulin 15, soybean polysaccharide 20, oat bran 25, dextrin 10, super Fine dietary fiber 10.

本发明的降糖复配米加工工艺见图1,包括步骤,The hypoglycemic compound rice processing technology of the present invention is shown in Fig. 1, comprises steps,

1)混匀:将上述原料投入混料机混合均匀,1) Mixing: Put the above raw materials into the mixer and mix evenly.

2)双螺杆多效热压工艺参数如下:螺杆转速120rpm,机筒温度依次为1效热压区60℃,2效热压区65℃,3效热压区80℃,4效热压区110℃,5效热压区90℃,6效热压区70℃;喂料转速为45rpm,液体泵转速为45rpm;2) The parameters of the twin-screw multi-effect hot pressing process are as follows: screw speed 120rpm, the temperature of the barrel is 60°C for the first-effect hot-press zone, 65°C for the second-effect hot-press zone, 80°C for the third-effect hot-press zone, and 80°C for the fourth-effect hot-press zone 110°C, 5-effect hot-press zone 90°C, 6-effect hot-press zone 70°C; feeding speed is 45rpm, liquid pump speed is 45rpm;

3)切粒/成型:多效热压分子重构后的物料经切粒成型装置切割成大米粒形状的复配米。3) Pelletizing/shaping: The material after molecular reconstruction by multi-effect hot pressing is cut into compound rice in the shape of rice grains by a pelletizing and forming device.

4)老化和干燥:老化工艺温度6±2℃,时间10小时。4) Aging and drying: the aging process temperature is 6±2°C, and the time is 10 hours.

其他操作同实施例1。Other operations are the same as in Example 1.

实施例3-9、Embodiment 3-9,

配方见表1,其中基础复配粉为籼米粉60,马铃薯粉40;加工方法同实施例2。The formula is shown in Table 1, wherein the basic compound powder is 60 of indica rice flour and 40 of potato flour; the processing method is the same as in Example 2.

体外预测血糖生成指数的检测方法为:称取经粉碎过70目筛的复配米粉试样100mg于50mL离心管中,一式3份。分别加入2mL的0.05M盐酸/瓜尔豆胶溶液和10mg蛋白酶,涡旋器混匀震荡,37℃水浴震荡孵育30min(震荡转速150rpm),再加入4mL 0.5M醋酸钠缓冲溶液(pH值5.2),漩涡混匀,在37℃水浴震荡孵育5min(震荡转速150rpm),然后加入混合酶液1mL(以25mL混酶液配置为例:称取α-淀粉酶3.6g于烧杯中,加入32ml蒸馏水,磁力搅拌器搅拌5min然后离心10min(离心转速1500g),取上清液21.6mL于烧杯中;取2.56mL淀粉葡糖苷酶稀释至3.2mL,取稀释后的淀粉葡糖苷酶2.4mL至装有α有淀粉酶上清液的烧杯中,再在此烧杯中加入转化酶1.6mL(1.6mg加1.6mL水)),漩涡混匀,在37℃水浴震荡孵育(震荡转速150rpm),严格记时,并分别在30、60、90、120、150、180min时取样100μL水解液于2mL离心管中,取样前要涡旋混匀,取得的水解液加入50%乙醇溶液1mL,混匀离心10min(离心转速8000g)。取离心后上清液100μL于10mL离心管中,再加入3mL葡糖氧化酶/过氧化物酶试剂(GOPOD),50℃孵育20min。其中空白为100μL蒸馏水与3mL GOPOD在50℃孵育20min。取出孵育后的样品(包括空白)每个吸取200μL于酶标板上,使用酶标仪在510nm处测其吸光度值。The test method for predicting the glycemic index in vitro is as follows: weigh 100 mg of the compound rice flour sample that has been crushed and passed through a 70-mesh sieve into a 50 mL centrifuge tube, and make triplicate. Add 2mL of 0.05M hydrochloric acid/guar gum solution and 10mg of protease, vortex to mix and shake, and incubate in a 37°C water bath with shaking for 30min (oscillating speed 150rpm), then add 4mL of 0.5M sodium acetate buffer solution (pH 5.2) , vortex to mix, and incubate in a 37°C water bath for 5 minutes with shaking (oscillation speed 150rpm), then add 1mL of mixed enzyme solution (take 25mL mixed enzyme solution as an example: weigh 3.6g of α-amylase in a beaker, add 32ml of distilled water, Stir with a magnetic stirrer for 5 minutes and then centrifuge for 10 minutes (centrifugal speed 1500g), take 21.6mL of supernatant in a beaker; take 2.56mL of amyloglucosidase and dilute to 3.2mL, take diluted amyloglucosidase 2.4mL until filled with α Add 1.6mL invertase (1.6mg plus 1.6mL water) to the beaker with amylase supernatant, vortex to mix, shake and incubate in a water bath at 37°C (oscillating speed 150rpm), strictly record the time, And sample 100μL of hydrolyzate in 2mL centrifuge tube at 30, 60, 90, 120, 150, 180min respectively, and vortex and mix before sampling. Add 1mL of 50% ethanol solution to the obtained hydrolyzate, mix well and centrifuge for 10min (centrifugation Speed 8000g). Take 100 μL of supernatant after centrifugation into a 10 mL centrifuge tube, add 3 mL of glucose oxidase/peroxidase reagent (GOPOD), and incubate at 50°C for 20 min. The blank was incubated with 100 μL of distilled water and 3 mL of GOPOD at 50° C. for 20 min. Take out the incubated samples (including the blank) and pipette 200 μL each onto a microplate plate, and use a microplate reader to measure the absorbance value at 510 nm.

葡萄糖标准曲线标制备:吸取25、50、75和100μL标准葡萄糖溶液,不够100μL的添加蒸馏水至100μL。再加入3mL GOPOD在50℃孵育20min。使用酶标仪510nm处测定吸光度值,并绘制标准曲线。Glucose standard curve standard preparation: draw 25, 50, 75 and 100 μL standard glucose solutions, add distilled water to 100 μL if 100 μL is not enough. Then add 3mL GOPOD and incubate at 50°C for 20min. The absorbance value was measured at 510nm using a microplate reader, and a standard curve was drawn.

根据葡萄糖标准曲线求取每个样品水解后的葡萄糖含量(G),然后按照水解率(HR)计算公式计算样品的HR。以水解时间为横坐标,以HR为纵坐标,绘制得到水解率曲线,采用Origin软件中的积分求水解率曲线下面积(AUC),按照水解指数(HI)计算公式计算样品的HI。Calculate the glucose content (G) of each sample after hydrolysis according to the glucose standard curve, and then calculate the HR of the sample according to the calculation formula of the hydrolysis rate (HR). With the hydrolysis time as the abscissa and HR as the ordinate, the hydrolysis rate curve was drawn, and the area under the hydrolysis rate curve (AUC) was calculated by the integral in the Origin software, and the HI of the sample was calculated according to the hydrolysis index (HI) calculation formula.

HR(%)=G×(7/0.1)×(1.1/0.1)×(1/1000)×(100/DM)×(162/180)HR(%)=G×(7/0.1)×(1.1/0.1)×(1/1000)×(100/DM)×(162/180)

上式中:G=葡萄糖质量(μg);7/0.1=在不同水解时间体积校正;1.1/0.1=GOPOD步骤体积校正;1/1000=葡萄糖从微克到毫克的转换;DM=样品干重(mg);162/180=从淀粉获得的游离D-葡萄糖转换到淀粉中存在的脱水D-葡萄糖因子。In the above formula: G=glucose quality (μg); 7/0.1=volume correction at different hydrolysis times; 1.1/0.1=GOPOD step volume correction; 1/1000=glucose from microgram to milligram conversion; DM=sample dry weight ( mg); 162/180 = conversion of free D-glucose obtained from starch to anhydro-D-glucose factor present in starch.

HI(%)=AUC1/AUC0×100 公式(1)HI(%)=AUC1/AUC0×100 Formula (1)

其中,AUC1=试样中淀粉的水解率曲线下面积;AUC0=标准样白面包水解率曲线下面积(12693.54)。Wherein, AUC1=the area under the curve of the hydrolysis rate of starch in the sample; AUC0=the area under the curve of the hydrolysis rate of standard sample white bread (12693.54).

最后,依据预测血糖生成指数公式GI=0.862HI+8.1981,计算GI值,3次结果求平均值。Finally, calculate the GI value according to the predicted glycemic index formula GI=0.862HI+8.1981, and calculate the average value of the three results.

表1:降糖复配米的蒸煮损失、体外预测血糖生成指数测定值Table 1: Cooking loss of hypoglycemic compound rice, measured value of in vitro predicted glycemic index

Wister大鼠降血糖试验:Hypoglycemic test in Wister rats:

空白对照组:正常Wister大鼠喂食正常饲料;Blank control group: normal Wister rats fed normal diet;

模型对照组:2型糖尿病模型Wister大鼠喂食正常饲料;Model control group: type 2 diabetes model Wister rats were fed normal diet;

实验组1:2型糖尿病模型Wister大鼠喂食降糖复配米饲料(实施例2的复配米);Experimental group 1: Type 2 diabetes model Wister rats were fed a hypoglycemic compound rice feed (the compound rice in Example 2);

实验组2:2型糖尿病模型Wister大鼠喂食市售米饲料。Experimental group 2: Type 2 diabetes model Wister rats were fed with commercially available rice feed.

附图2为降糖复配米对2型糖尿病模型Wister大鼠的降血糖效果。Accompanying drawing 2 is the hypoglycemic effect of the hypoglycemic compound rice on type 2 diabetes model Wister rats.

试验结果表明,本发明的降糖复配米蒸煮损失为11.6-14.5%,体外预测血糖生成指数为38.1-50.4,属于低血糖生成指数食物。The test results show that the cooking loss of the hypoglycemic compound rice of the present invention is 11.6-14.5%, and the predicted glycemic index in vitro is 38.1-50.4, which belongs to low glycemic index food.

本发明的降糖复配米对2型糖尿病模型Wister大鼠有显著的降血糖效果,食用本发明的降糖复配米35天后,2型糖尿病模型Wister大鼠的血糖值由26.1mmol/L降低至13.6mmol/L(附图2)。The hypoglycemic compound rice of the present invention has a significant hypoglycemic effect on type 2 diabetes model Wister rats, and after eating the hypoglycemic compound rice of the present invention for 35 days, the blood sugar level of type 2 diabetes model Wister rats is increased by 26.1mmol/L Reduced to 13.6mmol/L (accompanying drawing 2).

以上的实施例仅仅是对本发明的优选实施方式进行描述,并非对本发明的范围进行限定,在不脱离本发明设计精神的前提下,本领域普通工程技术人员对本发明的技术方案作出的各种变型和改进,均应落入本发明的权利要求书确定的保护范围内。The above embodiments are only descriptions of preferred implementations of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, ordinary engineers and technicians in the field may make various modifications to the technical solutions of the present invention. and improvements, all should fall within the scope of protection determined by the claims of the present invention.

Claims (9)

1. a kind of hypoglycemic compounding rice, it is characterised in that be made of the raw material including following mass fraction through hot pressing:Basis is multiple With 100 parts of powder, 5~25 parts of inulin, 10~40 parts of polysaccharide, 15~50 parts of oat bran, 5~20 parts of dextrin, dietary fiber 5~15 Part;Wherein described basis compounding powder is made of rice meal and dehydrated potato powder.
2. hypoglycemic compounding rice according to claim 1, it is characterised in that the basis compounding powder is by rice meal 45~65 35~55 parts of compositions of part and dehydrated potato powder, the rice meal is long rice flour or polished rice powder.
3. hypoglycemic compounding rice according to claim 1, it is characterised in that the polysaccharide for soybean polyoses, tremella polysaccharides, One or more in laminarin, panaxan, Radix Phodiolae Polyose.
4. hypoglycemic compounding rice according to claim 1, it is characterised in that the dietary fiber is ultra-fine dietary fiber, Granularity is 150~250 mesh.
5. according to the hypoglycemic compounding rice of Claims 1 to 4 any one of them, it is characterised in that be by including following mass parts Several raw materials are made through hot pressing:Basis 100 parts of powder of compounding, 15~25 parts of inulin, 15~25 parts of polysaccharide, 20~25 parts of oat bran, 20 parts of dextrin, 10~15 parts of dietary fiber.
6. the processing method of any one of Claims 1 to 5 hypoglycemic compounding rice, it is characterised in that including step:
(1) hot pressing of twin-screw multiple-effect, technological parameter are as follows:Screw speed 80-130rpm, barrel zone temperature are followed successively by 1 effect hot pressing area 50-70 DEG C, 2 55-85 DEG C of effect hot pressing areas, 3 60-90 DEG C of effect hot pressing areas, 4 90-120 DEG C of effect hot pressing areas, 5 65-90 DEG C of effect hot pressing areas, 6 45-70 DEG C of effect hot pressing areas;
(2) aging and drying:The material that hot pressing obtains passes through aging technique, 3-10 DEG C of the temperature of aging, when time 6-12 is small;So After dry, dry temperature be 30~60 DEG C, when drying time 10~15 is small.
7. processing method according to claim 6, it is characterised in that feeding during step (1) twin-screw multiple-effect hot pressing turns Speed is 25-65rpm, and liquid revolution speed is 20-55rpm, and pelletizing rotating speed is 45-80rpm.
8. processing method according to claim 6, it is characterised in that after step (1) twin-screw multiple-effect hot pressing, material is passed through Pellet device is cut into rice kernel shape shape.
9. according to claim 6~8 any one of them processing method, it is characterised in that the drying described in step (2) is three sections It is dry, wherein one section of dry temperature is 50 DEG C, humidity 80%, when the time 1 is small;The temperature of two-stage drying is 55 DEG C, humidity 85%, time 2 h;Three sections of dry temperature are 35 DEG C, humidity 70%, when the time 10 is small.
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