CN107922956A - Glycerine derived from staphylococcus epidermis produces accelerating agent, the antimicrobial peptide derived from skin epidermis keratinocyte produces accelerating agent and their applications in skin sparing external preparation - Google Patents

Abstract

The present invention provides a skin protectant and an external agent for skin protection, which not only act synergistically with a combination of plant polysaccharides, but also have antimicrobial peptides that promote the production of glycerol derived from Staphylococcus epidermidis and derived from skin epidermal keratinocytes produced an effect, and exhibited an excellent effect of improving touch when applied to the skin, wherein the Staphylococcus epidermidis is a useful skin-resident bacterium on the skin. More specifically, the present invention provides a skin protection agent and an external preparation for skin protection comprising lactic acid bacteria belonging to the genus Enterococcus.

Description

Glycerol production accelerator derived from Staphylococcus epidermidis, derived from epidermal horn of skin Antimicrobial peptide production enhancers of plasmogenic cells and their use in skin protection Application in external preparations

field of invention

The present invention relates to a pharmaceutical and an external preparation for skin protection by promoting the production of glycerol derived from skin-resident bacteria, Staphylococcus epidermidis, which is a beneficial bacterium, and promoting antimicrobial peptides derived from skin epidermal keratinocytes One or a series of functions produced to protect the skin from external stressors such as dryness and harmful bacteria.

Background technique

On human skin, commensal microorganisms form a population as skin-resident flora. As skin-resident bacteria, Staphylococcus epidermidis, Staphylococcus aureus, corynebacteria bacteria, etc. reside on the skin, and the function of protecting the skin from external damage is maintained by the balance of these bacteria. However, it is known that the balance of these bacterial flora is due to exposure to environmental factors such as rapid temperature changes, drying or strong detergents or chemical agents or physical/mental factors such as injury, emotional stress or poor general condition due to disease are damaged and thus this protective function is greatly reduced (Non-Patent Document 1).

For example, Staphylococcus epidermidis, which is known as a skin beneficial bacterium, decomposes sebum to produce glycerol, and thus plays a role in physical protective functions such as inhibiting dry skin and inhibiting the growth of miscellaneous bacteria by adjusting the pH of the skin surface. Therefore, when the proportion of S. epidermidis is reduced, these protective functions may no longer be exhibited, and this may lead to skin diseases. In order to suppress such skin diseases, moisturizing agents, antibacterial agents, bactericidal components, etc. are widely used in skin external preparations; however, depending on the drug components, there is concern about the protective function provided by Staphylococcus epidermidis which is an aerobic bacterium Can also be suppressed.

In addition, Staphylococcus epidermidis also plays a role in enhancing the biological protection function of the living body by stimulating the skin epidermal keratinocytes and promoting their production of antimicrobial peptides. As antimicrobial peptides produced by skin epidermal keratinocytes, human β-defensin-2 (hBD2) and human β-defensin-3 (hBD3) are known, and it has been reported that these antimicrobial peptides show Antibacterial action of Staphylococcus aureus, which is a harmful bacterium, and these antimicrobial peptides serve as a biological barrier to protect the skin from such harmful bacteria (Non-Patent Document 2).

Conventionally, in order to promote the proliferation of Staphylococcus epidermidis, sugars such as glucooligosaccharides are used as an assimilable source (Patent Document 1); however, due to not only useful Staphylococcus epidermidis but also other harmful bacteria such as aureus Staphylococcus aureus also assimilates such sugars, so the skin protective function cannot be obtained in some cases. In addition, many sugars have a sticky feel when applied to the skin, which presents another problem of tactile formulation difficulties.

Staphylococcus aureus is known to be abundantly present on the skin of people with dry or rough skin and atopic dermatitis patients. Toxins produced by Staphylococcus aureus are also known to cause further deterioration of skin conditions. In order to keep the skin surface condition healthy, it is therefore considered important to effectively kill Staphylococcus aureus and maintain Staphylococcus epidermidis having a skin protective function.

The action of Staphylococcus epidermidis on the skin is similar to that of lactic acid bacteria and bifidobacteria, which are useful gut bacteria. These useful intestinal bacteria are known to regulate intestinal pH, inhibit proliferation of harmful bacteria such as Clostridium perfringens and Escherichia coli, and have a protective function in the intestinal tract.

In order to improve the intestinal environment, useful bacteria such as lactic acid bacteria and bifidobacteria are ingested into the intestinal tract; however, in general, in order for these useful bacteria to reach and colonize the intestinal tract, acid-resistant strains need to be selected and included Enclosed in enteric-coated capsules. In addition, in order to increase the number of useful bacteria in the intestine, a method of ingesting food or drink containing dietary fiber and various oligosaccharides selectively assimilated by useful bacteria has also been employed. However, depending on individual constitutions, these methods sometimes cause excessive intestinal fermentation and diarrhea. As a method for solving such a problem, heat-killed cells using lactococcus have been proposed (Patent Document 2).

The heat-killed cells of Enterococcus faecalis ingested in the form of food or beverage can promote the proliferation of bifidobacteria in the intestinal tract, thereby inhibiting the proliferation of harmful bacteria and improving the protective function of the intestinal tract. Moreover, since the dead bacterial cells are easily processed into food or drink, unnecessary fermentation is not performed, and thus the storage stability of the food or drink is hardly affected.

Preferable examples of heat-killed cells of lactic acid bacteria include heat-killed cells of microorganisms classified as Enterococcus faecalis, and it is particularly preferable to use Enterococcus faecalis EC-12 strain (deposit number FERM BP-10284), whose 16S rDNA is in National Genetic The accession number in the institute is "AB15482".

prior art literature

patent documents

[Patent Document 1]: Japanese Unexamined Patent Publication No. 2005-002087

[Patent Document 2]: Japanese Unexamined Patent Publication No. 2004-051530

non-patent literature

[Non-Patent Document 1] Gallo, R.L. et al., Journal of Investigative Dermatology 131: 1974-1980 pages (2011)

[Non-Patent Document 2] Kisich, K.O. et al., Journal of Investigative Dermatology 127: 2368-2380 (2007)

Summary of the invention

The problem to be solved by the present invention

To maintain a healthy skin surface state, means and methods of protecting the skin from external stressors such as dryness and harmful bacteria are essential. An object of the present invention is to provide a pharmaceutical and an external preparation for skin protection by promoting the production of glycerin derived from Staphylococcus epidermidis and the production of antimicrobial peptides derived from skin epidermal keratinocytes against harmful bacteria such as Staphylococcus aureus One or a series of functions to protect the skin from external stressors such as dryness and harmful bacteria.

means of solving problems

The inventors of the present invention investigated various lactic acid bacteria and conducted in-depth studies. As a result, they found that cells of Enterococcus faecalis EC-12, especially heat-killed cells of Enterococcus faecalis EC-12, which are lactic acid bacteria, have the effect of promoting Glycerol production derived from Staphylococcus epidermidis and role in promoting production of antimicrobial peptides derived from skin epidermal keratinocytes. In addition, it was also found that heat-killed cells of Enterococcus faecalis EC-12 had an excellent silky feel when applied to the skin, and provided sensory benefits in any water-type, oil-type, emulsion-type, and powder-type topical preparations. Improvement effect (feeling-improving effect).

That is, the present invention includes the following items.

[1] A glycerol production accelerator derived from Staphylococcus epidermidis, the accelerator comprising lactic acid bacteria belonging to the genus Enterococcus as an active ingredient.

[2] The glycerin production accelerator derived from Staphylococcus epidermidis according to item [1], wherein the lactic acid bacterium belonging to the genus Enterococcus is Enterococcus faecalis.

[3] The glycerol production promoter derived from Staphylococcus epidermidis according to item [1] or [2], wherein the lactic acid bacterium belonging to the genus Enterococcus is Enterococcus faecalis EC-12 (Accession No. FERM BP-10284).

[4] The glycerol production promoter derived from Staphylococcus epidermidis according to any one of items [1] to [3], wherein lactic acid bacteria belonging to the genus Enterococcus are killed.

[5] The glycerol production accelerator derived from Staphylococcus epidermidis according to any one of items [1] to [4], wherein the lactic acid bacteria belonging to the genus Enterococcus are heat-killed.

[6] An antimicrobial peptide production accelerator derived from skin epidermal keratinocytes, the accelerator comprising lactic acid bacteria belonging to the genus Enterococcus as an active ingredient.

[7] The antimicrobial peptide production accelerator derived from skin epidermal keratinocytes according to item [6], wherein the lactic acid bacterium belonging to the genus Enterococcus is Enterococcus faecalis.

[8] The antimicrobial peptide production promoter derived from skin epidermal keratinocytes according to item [6] or [7], wherein the lactic acid bacterium belonging to the genus Enterococcus is Enterococcus faecalis EC-12 (Accession No. FERM BP -10284).

[9] The skin epidermal keratinocyte-derived antimicrobial peptide production accelerator according to any one of items [6] to [8], wherein lactic acid bacteria belonging to the genus Enterococcus are killed.

[10] The skin epidermal keratinocyte-derived antimicrobial peptide production accelerator according to any one of items [6] to [9], wherein the lactic acid bacteria belonging to the genus Enterococcus are heat-killed.

[11] The antimicrobial peptide production promoter according to any one of items [6] to [10], wherein the antimicrobial peptide is human β-defensin-2 (hBD2) or human β-defensin- 3 (hBD3).

[12] An external preparation for skin protection, comprising the glycerin production promoter derived from Staphylococcus epidermidis or the antimicrobial peptide production promoter derived from skin epidermal keratinocytes according to any one of items [1] to [11] agent.

Invention effect

The pharmaceutical agent and the external preparation for skin protection according to the present invention are capable of promoting the production of glycerol derived from Staphylococcus epidermidis and the production of human defensin-2 and human β-defensin-3, which are human β-defensin-2 and human β - Defensin-3 is an antimicrobial peptide against Staphylococcus aureus derived from epidermal keratinocytes of the skin. That is, it inhibits dryness of the skin by promoting glycerin production, and enhances the protective function of the skin, such as inhibiting the proliferation of miscellaneous bacteria, by controlling the pH of the skin surface. At the same time, by promoting the production of antimicrobial peptides against Staphylococcus aureus from epidermal keratinocytes of the skin, it is possible to protect the skin from the presence of harmful bacteria on it. Thus, through one or a series of these promoting functions, the surface condition of the skin can be kept healthy. In addition, the agent and the external preparation for skin protection of the present invention provide an excellent silky feeling when applied to the skin, and provide sensory improvement effects in any water type, oil type, emulsion type and powder type external preparations.

Brief description of the drawings

[Fig. 1] Fig. 1 shows the quantification of the heat-killed cells of Enterococcus faecalis EC-12 and the polysaccharide of Aloe vera (Aloe vera) as a plant polysaccharide added to the culture solution of the type strain of Staphylococcus epidermidis (ATCC 12228). Results of glycerol production.

[ Fig. 2] Fig. 2 shows the results of quantifying the amount of glycerol produced after the heat-killed cells of Enterococcus faecalis EC-12 were added to the culture solution of skin-resident bacteria collected from the glabellar area of a person.

[ Fig. 3] Fig. 3 shows the results of quantifying the amount of glycerol collected from human cheeks in a human subject test using a softening lotion containing Enterococcus faecalis EC-12 heat-killed cells.

[Fig. 4] Fig. 4 shows the measurement of human β-defensin-2 (hBD2) and human Results of the expression level of β-defensin-3 (hBD3) mRNA.

Description of the implementation

Ways of Carrying Out the Invention

The glycerol production accelerator derived from Staphylococcus epidermidis, the antimicrobial peptide production accelerator derived from skin epidermal keratinocytes, and the external preparation for skin protection of the present invention contain lactic acid bacteria belonging to the Enterococcus genus as active ingredients. In a preferred embodiment, the lactic acid bacterium belonging to the Enterococcus genus is Enterococcus faecalis, in particular heat-killed Enterococcus faecalis EC-12.

The medicament and the external preparation for skin protection according to the present invention have the following characteristics:

1. Promote the production of glycerol derived from Staphylococcus epidermidis;

2. Synergistically promoting the production of glycerol derived from Staphylococcus epidermidis by further combining plant polysaccharides;

3. Promote the production of antimicrobial peptides derived from skin epidermal keratinocytes against harmful bacteria such as Staphylococcus aureus;

4. Enhanced protection of the skin from external stressors such as dryness and harmful bacteria through one or a combination of the aforementioned promoting functions; and

5. Imparts an excellent silky feeling when applied to the skin, and can provide a sensory-improving effect in any of water-type, oil-type, emulsion-type, and powder-type external preparations.

There is no particular limitation on the method of culturing lactic acid bacteria belonging to the genus Enterococcus, including conventionally known lactic acid bacteria culturing methods, and heat-killed cells thereof can be produced by the following method. From a culture of lactic acid bacteria belonging to the genus Enterococcus obtained by a conventional method, bacterial cells are recovered by a method such as filtration or centrifugation, and then, after washing the recovered cells with water, the cells are suspended in water or the like, subjected to heat treatment, Concentration and drying are then carried out as necessary, whereby heat-killed cells of lactic acid bacteria belonging to the genus Enterococcus can be prepared. A generally used spray dryer, freeze dryer, etc. can be used. Depending on circumstances, before or after sterilization treatment by heating or the like, or before or after drying treatment, the recovered cells may also be treated with enzyme treatment, surfactant treatment and/or grinding/pulverization, and the obtained Products are also included in the invention of killed bacterial cells or products of their treatment.

Examples of water used for washing and suspending include pure water, tap water, well water, mineral water, mineralized water, hot spring water, spring water, and fresh water, and various treatment products thereof. Examples of water treatment include purification, heating, sterilization, filtration, ion exchange, osmotic adjustment, and buffering, among others. Therefore, examples of water that can be used as an extraction solvent in the present invention also include purified water, hot water, ion-exchanged water, physiological saline, and various buffers.

The lactic acid bacteria belonging to the genus Enterococcus obtained in the above manner can be used as a glycerol production accelerator derived from Staphylococcus epidermidis, an antimicrobial peptide production accelerator derived from skin epidermal keratinocytes against harmful bacteria such as Staphylococcus aureus, Or it can be used as a topical agent for skin protection to maintain a healthy skin surface condition through one or a series of these promoting functions.

In the glycerin production accelerator derived from Staphylococcus epidermidis and the antimicrobial peptide production accelerator derived from skin epidermal keratinocytes of the present invention, the above-mentioned heat-killed bacterial cells are preferably suspended and adjusted so that their amount is No less than 5×10 ¹¹ cells, particularly preferably no less than 5×10 ¹² cells in 1 gram of each accelerator. Furthermore, in conventionally used external preparations for skin protection, heat-killed bacterial cells are preferably incorporated and adjusted so that one-third to the same degree of the above amount is applied each time.

(Form of external preparation for skin protection)

The external preparation for skin protection of the present invention can take a form suitable for use such as ampoule, capsule, powder, granule, liquid, gel, bubble bubble, emulsion, sheet, mist or spray, and examples of such external preparation Including 1) pharmaceutical preparations; 2) quasi-drugs; 3) topical or systemic skin external preparations (for example, basic cosmetics such as lotions, lotions, creams, ointments, lotions, oils and masks; facial and skin cleansers , such as bar soap, liquid soap and hand wash; massage agent; cleaning agent; depilatory agent; depilatory agent; shaving agent; after-shave lotion; eyeshadows, eyeliners, and mascaras; perfumes; nail polishes; nail enamel; manicure glaze removers; poultices; plasters; tapes; sheets; patches; and aerosols); 4) medicinal and and/or cosmetic preparations (e.g. shampoos, rinses, hair treatments, hair pre-treatments, long-lasting solutions, hair dyes, hair conditioners, hair tonics, hair growth/restoration stimulants, poultices, plasters, tapes , tablets and aerosols); 5) body washes to drop into the bathtub; and 6) other preparations such as underarm deodorants, deodorants, antiperspirants, hygiene products, sanitary napkins and wet wipes.

(Ingredients of external preparations for skin protection)

In addition, these external preparations for skin protection can be arbitrarily selected according to need and produced using those components and additives exemplified below. The amount incorporated in these preparation systems is not particularly limited; but it is generally considered to be preferably about 0.0001-50%.

(1) Various oils and fats

Examples of oils and fats include avocado oil, almond oil, anise oil, perilla oil, olive oil, orange oil, orange roughy oil, sesame oil, cocoa butter, chamomile oil, carrot oil, Cucumber Oil, Tallow Fatty Acids, Macadamia Nut Oil, Safflower Oil, Shea Butter, Liquid Shea Butter, Soybean Oil, Camellia Oil, Corn Oil, Rapeseed Oil, Peach Kernel Oil, Castor Oil, Cottonseed Oil, Peanut Oil, Turtle Oil, Mink Oil, egg butter, palm oil, palm kernel oil, haze wax, coconut oil, tallow, lard, squalene, squalane, pristane, and hydrogenated products of these oils and fats (e.g. hardened oil).

(2) Waxes

Examples of waxes include beeswax, carnauba wax, spermaceti, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax, montan wax, shellac wax and rice bran wax.

(3) Mineral oil

Examples of mineral oils include liquid paraffin, petrolatum, paraffin, ozokerite, ceresin and microcrystalline wax.

(4) fatty acids

Examples of fatty acids include natural fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, linoleic acid, linolenic acid, docosahexaenoic acid, eicosapentaenoic acid, acid, 12-hydroxystearic acid, undecylenic acid, tall oil and lanolin fatty acids; and synthetic fatty acids such as isononanoic acid, caproic acid, 2-ethylbutyric acid, isovaleric acid, 2-methyl Valeric acid, 2-ethylhexanoic acid and isovaleric acid.

(5) Alcohols

Examples of alcohols include natural alcohols such as ethanol, isopropanol, lauryl alcohol, cetyl alcohol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, phytosterols, and phenoxyethanol; and synthetic alcohols such as 2 - Hexyldecanol, Isostearyl Alcohol and 2-Octyldodecanol.

(6) Polyols

Examples of polyhydric alcohols include ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol Glycol, propylene oxide, propylene glycol, polypropylene glycol, 1,3-butanediol, pentylene glycol, glycerin, pentaerythritol, threitol, arabitol, xylitol, ribitol, galactitol, sorbitol , mannitol, lactitol and maltitol.

(7) Esters

Examples of esters include isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyl myristate Lauryl ester, hexyldecyl dimethyl octanoate, cetyl lactate, myristyl lactate, diethyl phthalate, dibutyl phthalate, acetylated lanolin, ethylene glycol Alcohol Monostearate, Propylene Glycol Monostearate, and Propylene Glycol Dioleate.

(8) Metal soaps

Examples of metallic soaps include aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate and zinc undecylenate.

(9) Colloids, sugars and water-soluble polymers

Examples of gums, sugars, and water-soluble polymers include gum arabic, benzoin gum, dammar gum, guaiac resin, Irish moss, karaya gum, tragacanth gum, locust bean gum, quince seeds, agar, Casein, lactose, fructose, sucrose and its esters, trehalose and its derivatives, dextrin, gelatin, pectin, starch, carrageenan, carboxymethyl chitin and chitosan, added with epoxy Hydroxyalkyl (C ₂ to C ₄ ) chitin and chitosan of alkanes (C ₂ to C ₄ ) such as ethylene oxide, low molecular weight chitin and chitosan, chitosan salts, sulfuric acid Phosphorylated chitin and chitosan, phosphorylated chitin and chitosan, alginic acid and its salts, hyaluronic acid and its salts, chondroitin sulfate and its salts, heparin, ethyl cellulose, methyl cellulose , carboxymethyl cellulose, carboxyethyl cellulose, sodium carboxyethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether , polyvinylpyrrolidone, polyvinylmethacrylate, polyacrylates, polyalkylene oxides such as polyethylene oxide and polypropylene oxide, cross-linked polymers thereof, carboxyvinyl polymers, and polyethyleneimine.

(10) Surfactant

Examples of surfactants include anionic surfactants (alkyl carboxylates, alkyl sulfonates, alkyl sulfates, and alkyl phosphates); cationic surfactants (alkylamine salts and alkyl quaternary ammonium salts) ; Amphoteric surfactants, such as carboxylic acid type amphoteric surfactants (amino type and betaine type); sulfate ester type amphoteric surfactants; sulfonic acid type amphoteric surfactants; phosphate ester type amphoteric surfactants; Active agents (ether nonionic surfactants, ether ester nonionic surfactants, ester nonionic surfactants, block polymer nonionic surfactants and nitrogen-containing nonionic surfactants) and other surface active agents Active agents (natural surfactants, protein hydrolyzate derivatives, polymeric surfactants, titanium silicon-containing surfactants and fluorinated carbon-based surfactants).

(11)Various vitamins

Examples of vitamins include vitamin A group, such as retinol, retinal (vitamin A1), dehydroretinal (vitamin A2), carotene and lycopene (provitamin A), etc.; vitamin B group, such as hydrochloric acid Thiamine, Thiamine Sulfate (Vitamin B1), Riboflavin (Vitamin B2), Pyridoxine (Vitamin B6), Cyanocobalamin (Vitamin B12), Folic Acid, Niacin, Pantothenic Acid, Biotin, Choline and inositol; vitamin C group, such as vitamin C acid and its derivatives; vitamin D group, such as ergocalciferol (vitamin D2), cholecalciferol (vitamin D3) and dihydrotachysterol; vitamin E group, such as vitamin E , its derivatives, and ubiquinone; the vitamin K group, such as phytonadione (vitamin K1), menaquinone (vitamin K2), menadione (vitamin K3) and dihydronaphthoquinone (vitamin K4); and essential Fatty acids (vitamin F), carnitine, ferulic acid, gamma-oryzanol, orotic acid; vitamin P group (rutin, eriocitrin, and hesperidin); and vitamin U.

(12) Various amino acids

Examples of amino acids include valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, Serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid, hydroxylysine, arginine, ornithine, histidine, and amino acid derivatives such as sulfates, phosphates, nitrates, citrates and pyrrolidone carboxylic acids of the aforementioned amino acids.

(13) Various additives derived from plant or animal materials

Depending on the type and mode of the product to which the additive is added, after treatment with conventional methods (optionally such as pulverization, milling, washing, hydrolysis, fermentation, purification, pressing, extraction, classification, filtration, drying, pulverization, granulation , dissolving, sterilizing, adjusting pH, deodorizing, decolorizing, etc.) after the plant or animal material, the additives from the plant or animal material are arbitrarily selected and incorporated.

The solvent used for the extraction can be selected in consideration of the intended use and type of the product to which the additive is added and the subsequent processing/handling etc. propanol, butanol, isobutanol), aqueous lower alcohols, polyols (such as propylene glycol, 1,3-butanediol, glycerin), aqueous polyols and various organic solvents (such as acetone, ethyl acetate) species or a mixture of two or more. However, depending on the application, when the use of an organic solvent is not preferable, only water or only ethanol which is easily removed after extraction may be used or arbitrarily mixed with water, or an additive for extrusion extraction may be used.

Additives derived from plant or animal materials are expected to be incorporated into systemic or topical topical agents or cosmetics for not only cosmetic effects such as protection of skin and hair, moisturizing, improving touch and skin texture, providing softness, alleviating irritation, through Fragrance relieves stress, activates cells (prevents cell aging), suppresses inflammation, improves skin and hair condition, suppresses and improves rough skin, produces hair, promotes hair growth, suppresses hair loss, imparts luster, cleansing effect, relieves fatigue, promotes blood circulation and Bathing effect, also has functions such as fragrance enhancement, deodorization, thickening, antiseptic and buffering.

Also, since various cosmetic and pharmaceutical effects previously known from various raw materials are expected, by using these materials in combination, the expected effects of the present invention can be enhanced, and products expected to exhibit multifunctional effects can be obtained.

Specific plants (crude drugs) used as raw materials are, for example, almond (almond), Polygonum tinctorium Lour, Sinomenium acutum Rehder et Wilson, Japanese coral, sycamore or sycamore, Cocculus trilobus DC. , Gastrodia elata, acacia, red cohosh, red currant "fruit", red clover, red grapes, ink tree (sumac), wild sycamore (wild paulownia), red madder or madder or madder (madia root), red Rehmannia glutinosa Libos (Rehmannia glutinosa Libos.), Asafoetida, East Asian Tang Pine Grass, Ulmus elm (Um bark), Pouteriagardneriana, Palm mulberry, Akebia (Akebia), Hemp seeds, Purple morning glory or morning glory, Hydrangea ( Hydrangea), Ashitaba, Adzuki (red bean), Luo Hanbo, acerola, catechin (elixir), edible canna, anise, lucuma (Abiu), Abiurana, canola, cork oak "fruit" , avocado, flax, sweet tea, aescin, wilting (polygonatum odoratum), Shan Arthia (Guang Arthia), Amaranthus (Amaranthus) (Amaranthus, Anthracite, Amaranth, Fairy Valley, Amaranth, Green Spike Amaranth, Amaranthus retroflexus, Amaranthus amaranthus, Persimmon orchid, Chiselia), Lily or Fritillaria, Alstroemeria (Alstroemeria), Buglossia (Alkanna tinctoria), Mesquite (Algarrobo), Hollyhock, Arnica, Cardamom (Alpinia katsumadai), Aloe Vera, Aloe Vera, Angelica, Apricot (Almond), Benzoin, Lucuma, Echinacea, Berberis (Epimedium grandiflorum), Rush, Knotweed (Knotweed root ), Yew, Strawberry, Fig "fruit, leaf", Iris tectorum, Abutilon avicennae (mallow seed), wintergreen, Ginkgo "seed, leaf", Polygala tenuifolia, horn Beans, Drabanemorosa, Amaranthus blitum, Rice "seeds, seed coats", Anethum graveolens "seeds", Mountain Pepper (Thymus quinquecostatus), Nettles, Ylang-Ylang, Rock Lettuce (Conandron ramondioides), Selaginella or rock pine, green beans, oolong tea, fennel, Huito (Genipa Americana), wattle, warbler vine "fruit", Tibetan fennel (Carum carvi), turmeric (turmeric), Waterweed (Duckweed), Asarum or Asarum or Asarum (Asarum), Malva (Malva sylvestris), Marshmallow (Althaea officinalis), Ponerorchis graminifolia, Prunella vulgaris, Aralia cordata (notopterygium, tomentosa, tanghuo), cat's claw vine (Uncaria tomentosa), Uva tea, plum (Plum "seed, pulp"), white back Oak (Quercus stenophylla), bear grape leaf, Wenzhou citrus (tangerine peel), Wenpu, tansy, eleuthero, green persimmon, echinacea (Echinacea), gorse (Cytisusscoparius), mushroom (Flammulina velutipes ), Cassia (Senna obtusifolia) or Cassia (Sennaobtusifolia), Gentianella alborosea, Elderberry "fruit", Canarium luzonicum, Pleurotus eryngii, Sophora japonica (Sophora japonica, Sophora japonica), Pea, Astragalus, Polygonatum (Polygonatum falcatum), Queen Victoria Agave (Agave victoriae-reginae), Coptis chinensis, Prickly pear, Trichosanthes bracteata (including root), Inula helenium, Ivy (Fangji), Plantago (Psyllium), Rhaponticum uniflorum (Qizhou Leulah, Pulsatilla), Hazelnut (Corylus heterophylla), Giant Column (Carnegiea gigantes Br.et R.), Atractylodes macrocephala (Atractylodes macrocephala), Lagerstroemia speciosa), Alnus sieboldiana "fruit", Eriocaulonbuergerianum, Mamey fruit (Pouteria sapotae), Hawthorn, Lodoicea, barley, Sorrel (Oxalis tuberosa), snake Cnidium monnieri, Okra "fruit", Inula (Inula), Echinops latifolius (Blue thorn head), Panax ginseng or Panax ginseng, Forsythia or Hypericum perforatum or Hypericumperforatum (Diqiecao ), Wild Sesame (Dipsacus), Cocklebur (Xanthium), Juglans, Beetroot or Dioscorea (Dioscorea), Gastrodia elata, Lilium lancifolium or Lilium japonicum Or wild lily (Lily), Ogami Orchid (Orchis fauriei), Formoniosa, Ulmus laciniata, Asparagus, Quinoa officinale (Olluco), Watercress (Nasturtium officinale) (Cresson), Parsley (Petroselinum crispum), Psoralea corylifolia , Celery (Apium graveolensvar.dulce), Olive "Pulp, Seed, Leaf", Oregano, Orange "Fruit, Peel", Rehmannia glutinosaLibos.var.hueichingensis), Cocoa "Fruit, Peel, Seed", Persimmon (Hydrangea "leaf"), Uncaria (Fishing Vine Hook), Money Mint (Glechoma hederacea) or Lilynium, Persimmon Orchid, Hydrangea macrophylla, Cassia, Broussonetia papyrifera fruit"), curcuma, oak, quinoa, buckthorn bark, syringa, inula (Inula salicina), seaweed (Golden sand vine), quinoa, carnation, valerian, birch, birch or silver Betulapendula (white birch), gerbera, squash, jeep "seeds", Zanthoxylum bungeanum (Shujiao), cattail (Cathaceae), chamomile or Roman chamomile, chrysanthemum, Myrciaria dubia), calla lily, Fumaria officinalis, king melon (Trichosanthes cucumeroides), Pinellia ternata, oats, guarana "seeds", Magnolia officinalis, sycamore, Wolong, Papaya, Garcinia Cambogia, Agastache rugosa, Potentilla chinensis (Veronica undulata), Veronica undulata, Coriolus versicolor, Dianthus or Qumai (Quwheat), Artemisia apiacea ), Kawahara (Artemisia capillary), kansui, licorice, cantara agave, candelilla, coltsfoot (Tussilago farfara), canna, olive, raspberry (raspberry, dutchberry, white berry, Red plum, raspberry, red raspberry), kiwi "fruit, leaf", yellow squash (including Lougen), bellflower (bellflower root), chrysanthemum (chrysanthemum, cold chrysanthemum, Korean wild chrysanthemum), chamomile, catalpa Tree (Catheria chinensis), Latium chinensis (Redwort root), Citrus aurantium, Diamond leaf ivy, Aloe arborescens, Campanula (Satureja hortensis), Quinine, Quinine tree, Quinoa , Cortex Phellodendri (Phellodendron), Pigeon Peas, Gymnema sylvestre, Cereus peruvianus, Cabbage, Whole Cabbage "Unripe Fruit", Caraway Seeds, Cucumber, Tamarix (Tamarix), Soap Bark Tree (Quillaja saponalia), soap tree, stone pine (Golden sore grass), kumquat "fruit", scarab tree, camellia, golden fan cactus (Opuntia tuna), old vine (Piper betle), agaric (Agrimony), Golden Orchid, Silver Orchid, Golden Edge, Lucuma laurifolia, Guava "fruit", Gray Guayule (Parthenium Argentatum), Gall (Quercus infectoria), Macadamia, Cephalanthera longifolia, Goji Berry (Godberry, Lycium barbarum Leaf, Digubara), Asparagus, Pueraria (Pudzu), Camphor, Gooseberry "fruit", Artemisia annua (Artemisia annua leaf), P. macrophylla, Gardenia (Gardenia jasminoides), Oak, Quaint Aso (Theobroma grandiflorum), Cucumber, Kumasa, Verbena, Sophora flavescens, cranberry "fruit", chestnut "seed, pulp, astringent skin", curculigo glabra (Curculigo Latifolia) "fruit", Grapefruit "fruit, leaf", buckthorn, iron holly (Ilex rotunda), syringa, clove (Lilac), arhat (Schima, Schima), Nepeta (Schizonepea), cockscomb (Cockscomb Seedlings), Epiphyllum, Laurel, Smilax (Mountain Return), Gentian, Geranium (geranium), Hovenia dulcis (Hovenium dulcis), original Dendrobium nobile (Dendrobium nobile), Kizhou tangerine (Citrus kinokuni) (mandarin, tangerine, mandarin, satsuma, sagami, yuzu, orange peel), rose, melissa (Melissa officinalis), mulberry "fruit", black tea, pineapple (Chuan bone), hawon , sorghum, galangal, coriander "fruit", berberine, Scutellaria baicalensis (scutellaria baicalensis root), lingonberry, coconut "fruit", evodia, Rubus chingii (raspberry), pepper, Cosmos, Cuban balsam (Copaifera officinalis), coffee "seeds, leaves", magnolia or magnolia (Magnolia), burdock (Arctium aurtifolia), female wilt (Clematis apiifolia var. birnata), flax, scrophulariaceae, Schisandra, nan Schisandra or Schisandra bicolor, Wheat, Rice or Rice Bran "Red Bran, White Bran", Rice Bran Oil, Sudan Kola Nut "Seeds", Kola Nut "Seeds", Buxus Leaf vs. Echinacea (Excelsa), Fenugreek "Fruit" , African fangjigen, South American milk vine bark, kelp, konjac, comfrey, Qiongma, Caipei orchid, bird's nest orchid (Hokkaido bird's nest orchid), clematis chinensis (Clematis chinensis), cherry (Oshima cherry, mountain cherry, big mountain cherry, Edo Higan Sakura, Tomozakura, Miyama Sakura, Someiyoshino Sakura, Takamine Sakura, Kasumi Sakura, Small Higan Sakura, Satozakura, Hanzakura ("leaf, flower, fruit, bark (cherry bark)"), cherry, pomegranate , bamboo, Cephalanthera longibracteata, camellia, Alisma alisma (Alisma alisma), sweet potato, sugar cane, sugar beet, wild jujube (zizinus), saffron (saffron), sapodilla, egg yolk Pouteria campechiana (Pouteria campechiana), Cinnamon "fruit", Saponaria, American raspberry "fruit", Cimicifuga, Sage, Sage, Lobelia grandis (Lobelia), Juglans (Hickory), Maple poplar, dragon fruit, Peruvian dragon's blood (croton), three-branch nine-leaf clover, panax ginseng, dogwood, sansho, mountain bean root, avocado, avocado tree (Butyrospermum parkii), Japanese linden "fruit", shiitake mushrooms (shiitake) , aster, four-lens bean, digitalis, shijunzi, perilla or green perilla or burmese perilla or single-faced perilla (perilla leaf, perilla seed), red sandalwood, Japanese linden tree, magnolia officinalis (Magnolia officinalis ), chrysanthemum (forsythia), jitake mushroom (brown gray mushroom, smoke-colored chrysanthemum, lotus leaf chrysanthemum, silver-white chrysanthemum, Hongxi mushroom, true chrysanthemum chrysanthemum, jade mushroom), purple magnolia (Magnolia ), Spiraea, Potato, Coral Vegetable (Arrhythmia), Jasmine (Jasmine Flower), Step Grass (Ophiopogon japonicus), Long Cowpea, Gypsophila, Shrinking Sand (Amomum, Shrinking Sand), River Valley, Palm "Fruit", Chunlan, Queen's Sunflower, Ginger, Changshan, Calamus (calamus root), Cardamom "Fruit", Castanopsis "seed", Baiji, White Butterfly "seed", White Trifolium (Clover), Chicken Bone Grass (Abrus fruticulosus), sweet iris (Irisflorentina L.), white vine "flower", white lupine, Nandina domestica var. leucocarpa (nandina fruit), red cinchona succirubra, daphne (Daphnet Flower, Cinnamon), Gui, Cinnamon (Cinnamon Bark), Watermelon, Honeysuckle (Honeysuckle), Sorrel, Swimberry "fruit", Star Apple, Stevia, Strawberry "fruit", Bell Bupleurum, Equisetum (Wortaceae), Horse Tooth Amaranthus (purslane), plum "fruit", Jianlan (male orchid), Scotch pine "spore leaf ball", European larch, ivy, Persian walnut, Western hawthorn, Western dandelion, European horse chestnut (7 Leaf tree), Pear "fruit", Spiraea, Elderberry (Sambucus nigra), Juniper (Juniper), Achillea (Yarrow), Rosa chinensis, Capers, European mistletoe, Pepper Mint or lemon balm, horseradish, calamus (calamus root), dendrobium (dendrobium cherry, original spring dendrobium, large flower stone Dendrobium, Okinawa Dendrobium, Dendrobium officinale, Butterfly Dendrobium, Four-cornered Dendrobium, Yellow-flowered Dendrobium (Dendrobium), Lemon Verbena (Deodorant Wood), Mallow, Polygala broadleaf, Polygala, Cress, Celery, Rhizoma Chuanxiong , Andrographis paniculata, Sandalwood, Prickly Prickly Cactus, Senna "Fruit, Leaf", Grape Leaf Clematis (Great Polygonum), Herbal Medicine, Chuanpu, Tsaoguo, Lemaireocereus griseus B.et R., Suxin Wintersweet, Buckwheat" "Seed", yam, broad bean, rhubarb, radish, soybean, lime (wild orange peel, citrus aurantium), Huadong blue thorn (Southeast blue thorn), snakehead sausage (Eclipta), euphorbia, golden dog hair Fern (dog ridge), black sweet cherry "fruit", hollyhock, thyme (thyme), slender yam (Syrmus chinensis), standing willow, tamarind "seed", onion, magnolia (magnolia), aralia "fruit, leaves , root bark", big-leaf holly (one-leaf tea, Kudingcha, Kudengcha), dahlia (passiflora), variegated lupine, phlegm-cut bean, single-thorn cactus, salvia, dandelion or white-flowered dandelion or Mongolia Dandelion, dandelion, cherry "fruit", Imperata "fruit, root, shoot", bamboo ginseng, chicory, bead grass (Phyllanthus niruri), tulip, choko-no-stei, artichoke (Cynara scolymus), rhubarb ( Rhubarb), Catkin (Waterflower), Artemisia argyi (Artemisia argyi), Maitake (Polyporus), Cadida, Evening Primrose, Neottia kiusiana, Agaricus bisporus (mushroom), Tutongcao, Camellia, Gotu Kola, Clawgrass (Argentina), Commelina, Red Bean, Weeping Dendrobium, Polygonum multiflorum, Apricot, Four-leaved Ginseng, Wild Soybean, Ligwu, Erythrina, Common Walnut, Dewberry "fruit", Gemweed , sweet tea, Tiantai black medicine (black medicine), wax gourd (winter melon seeds), Chinese Uncaria, capsicum (pepper), angelica, calendula, acacia, chuanduan (chuanduan), corn or corn silk (maize silk) ), privet (privet lucidum), gentian or Korean gentian, horsetail, houttuynia, ipecac, dodder or kudzu, Eucommia "bark, leaf, root", ash (ash bark), tomato , astragalus, acacia "seeds", Eustoma, Potentilla, Liao Yang, hibiscus, tonka bean, Nigerian berry "fruit", house potato or yam, pseudoleaf tree, fenugreek, shepherd's purse, rapeseed, sword bean , lime, jujube (jujube), golden cherry, Dipsacus, fungus, peanut (groundnut), nandina (nandina fruit), ramie, pansy, bitter wood, wormwood, nutmeg, laurel or Cinnamon or Ceylon cinnamon or cinnamon (cinnamon bark) or cassia twigs, Orchis joo-iokiana, leeks (leeks), elderberry "fruit, flowers, stems, leaves", garlic (garlic), gallwood (gallia) , Onion, Albizia tree or Albizia or Hell Flower, Wild Thistle (Big Thistle), Wild Rose (Campus), Bignonia (Spirulina), Pheasant Comb, Yarrow, Earth Angelica (Fronthus), Wild Rose, Peach, Bromeliad "fruit", Hibiscus (Hibiscus, Chandelier Hibiscus, Hibiscus), Gentiana oblique, Lupin, Pau Dausset, Oak Fern (Drynaria), White Saffron vine, Dactylorhiza aristata, white huckleberry bark, cranberry "fruit", chickweed, hazelnut (hazelnut), scopolamine (scopolamine root), basil, lotus (lotus flesh, lotus seeds), parsley (parsley) , Rye, Sweet Potato, Light Bamboo or Bitter Bamboo (Bamboo Ru), Peppermint, Peppermint (Mint Leaf), Mucuna, Coix (Coix Seed), Anemarrhena, Banana, Oregano, Poppins (Trustis terrestris, Tribulus terrestris), Vanilla Bean, Papaya, Sageweed, Paharobobo, Bobobo "whole grass, stems, leaves", red pepper, succulent orchid, Vitex unileaf or Vitex three-leaf (Vitex), Cyperus cyperus, Tribulus terrestris ( Tribulus terrestris), rose (rose flower), coral dish (shore parsnip), witch hazel, palm, rose, balata, balata tree, edible toadstool (horse mushroom, globular mushroom), spider egg, Cyclocarya paliurus (Binaza jujube), Wall grass, Spring cold orchid, Bitter melon (Litchi vine), Spring elm (Ulmus bark, Ulmus bark, Um leaves), Niguma, Spring gentian, Fat sea, Bread tree, Shegan , Codonopsis Codonopsis (Codonopsis), pecan, Bianhua (lycoris, manjusawa), red dahlia, rough blue thorn head, water chestnut (lingshi), pistachio, sugar radish, stone reed, genista , Daisy (Marguerite), Achyranthes, Orchis chidori, Hinoki, Hinoki Leaf, Castor Bean, Sunflower, Bell Pepper, Geranium, Cattail, Agaricus blazei Murrill, Hilo Matsutake, Bird's Nest Orchid, Allspice Fruit "fruit", Angelica dahurica, Bletilla striata, amaranth "fruit", chickpeas, lentils, marshmallow, pulsatilla, loquat "fruit, leaves, stems", betel nut (big belly skin, betel nut), Puer tea (Puer tea ), Ivy vine (South vine), butterbur, coltsfoot dandelion (coltsfoot flower, coltsfoot leaf), euphemum (bluegrass), kidney bean (lentil), genkwa (Cherry japonica, genkwa), grape "fruit , Peel, Seed, Leaf", Beech, Cordyceps (Cordyceps), Brazilian Licorice, Brazilian Ginseng, Brazilian Coconut, Brazilian Ivory Nut, Blackcurrant "fruit", Blackberry, Pradosialactescens, Plum "fruit", Furcellariaceae, Blueberry (Vietnam Tangerine), Prune, Flora blanca, Psyllium, Bean (mung bean), Hazelnut, Loofah, Pineapple, Safflower, Agave japonica, Belladonna, Raspberry "fruit", Avocado, Pell LesbianBerg, Sakura Unicorn, sedum, California blackberry "fruit", Kochia or broom grass (Kochia chinensis), Kochi orchid, Impatiens (Impatiens, Impatiens, Sperancus), Mami crackers, lucuma, Japanese Magnolias , windproof, spinach, physalis, Japanese Magnolia (Pakistan), papaya, wild chrysanthemum (bitter barley), inula inula, atractylodes atractylodes, sedum sedum (sedum notoginseng), echinacea angustifolia, linden , peony "flowers, leaves, stems, bark", peony bark, Hubu, jojoba, borage, Bordeaux leaves, bird's beak berry "fruit", salt-born Cistanche (Cistanche, Dayun), Dendrobium officinale (Dendrobium huoshanense), Aracea pinnata (Aracea), Maitake, Ephedra, Maca, Macadamia, Margaret Flower (Wooden Spring Chrysanthemum), Partridge (Seaman's Grass), White Mulberry (Morus alba "bark, leaf"), Wudang magnolia, Balata tree, masaranzuba-do-seara, Sideroxylon rugosum, Tuberous nasturtium (Tropaeolum tuberosum) (nasturtium bulbum), Polygonum argentina, Pine cone, Pine "leaf, bark, root", Pouteria salicifolia, Poria cocos (Poria cocos), Peperaduncum angustifolia (Piperaduncum), Marjoram (Oregano), Willow leaf sand Ginseng (Sophora flavescens), Mulberry "fruit", Quince tree, Mullein (Verbascumthapsus), Mango, Mangosteen, Chamomile (Andean chamomile), Juglans, Citrus "fruit", Ganoderma lucidum, Citrus aurantii "fruit", Mishima Bupleurum (Bupleurum), Waterwheels (Agave), Lobelia (Lobelia chinensis), Japanese Chikuchi (Thousands), Bianzhu, Sleeping Vegetables, Duck Celery, Lumei Guolan, Spearmint, Mimosa, Mizuho, Miracle "fruit," Myrrh, Helichrysum, Helichrysum, Hibiscus, Sapinberry, Soapberry (Exantias bark), Munya, Lilac (Purple Root), Purple Pearl, or Japanese Purple Pearl , Purple Corn, Vitex Vitex (Kunming Millipede), Megakansa-Opuntika, Motherwort, Basil, Melaleuca, Melissa, Yellow Herb Melilotus, Melon "Fruit", Mongolian Artemisia, Meng Zongzhu, Wood Unicorn, Frankincense (Schinus molle), holly, woody, rhubarb palmate, peach "leaf, seed, flower, fruit", bean sprouts, black cherry "fruit", long-capsulated jute (jute), purple sweet potato, puzzle, ghost lamp Cornflower (Cornflower), Peppermint, Yacon, Yacon galla (cornflower) or Weeping Alder or Port Arthur "fruit, peel, ear", bayberry, star anise gold plate, mistletoe (Willow parasite), Willow (willow willow, standing willow, weeping willow, glandular willow, pussy willow, cotton willow, silk willow, pointed leaf purple willow, cloud dragon willow, deep mountain willow, coconut, mountain willow, big-leaf willow, Artemisia willow, fox-colored willow (Salix vulpina ), Liao Yang), Polygonum salicifolia "leaves, stems", black berry, Zijinniu, Tianmingjing (crane lice, Tianmingjing), pokeweed, Liaodong alder (Alnus hirsuta var.sibirica), bayberry ( bayberry bark), mugwort, eucalyptus, saxifrage, yucca or eucalyptus, pomelo "fruit", lily, angelica, mugwort (wormwood leaf), ray pill, lime "fruit", rye, monk fruit "fruit" ", Rubus "leaf, fruit", Latania plantain (Krameriaceae), scallion or Ashkelon onion (Allium scallion), lavender, longan (longan meat), agave (Agave japonica, Agave versicolor orchid), green tea, apple "fruit, seed, leaf, root", gentian, raspberry or sweet tea raspberry, hard-leaved blue thorn head (glass jade thistle, Xinjiang blue thorn head), lychee (lychee core), lettuce, Red currant "fruit", red dragon fruit, lemon "fruit", lemongrass, Admiralty (forsythia), milk vetch, wintersweet, wax palm, reed root (reed), large Arbutus "fruit", rosemary, rosehip (Rosa briar), mountain cabbage, Budisma, Burnet, etc.

Representative examples of seaweeds include: seaweeds [green algae: Chlorella vulgaris, Chlorella pyrenoidsa, Chlorella ellipsoidea, Enteromorpha linza), Enteromorpha prolifera, Enteromorpha compressa, Enteromorpha intestinalis, Enteromorpha clathrata), Ulva pore (Ulva)], seaweed [brown algae: kelp ( True kelp (Saccharina japonica, Rishiri kelp, wrinkled kelp, narrow-leaved kelp), wakame, broad-leaved wakame, green wakame, macroalgae (Macrocystis pyrifera), macrocystis integrifolia, coelocystis (Nereocystisluetkeana)), Hijiki, Fucus, Padina australis, Padina australis var.cuneata, Padina australis var.cuneata, Padina australis var. cuneata, Padina australis var. , seaweed [Red algae: dilute centipede algae, alyssum, flat alyssum, Japanese alyssum, chicken feathers, winged vegetables, sagittella brittle, coarse alyssum, creeping cauliflower, cockscomb, sawtooth Eucheuma, pair Raw Eucheuma, Eucheuma, Cypress-shaped Eucheuma, Carrageen, Giant Carrageen, Chondrus crispus, Wedge Carrageen, Spiny Carrageen, Pinnae Carrageen, Longhorn Forkweed, heterochromatic carrageen, Japanese carrageen, pinnae carrageen, cedar spruce, red soft spiny spruce, small spruce sp. algae, red algae] and so on.

In addition, representative examples of seaweed also include other algae such as: green algae (Chlamydomonas: Chlamydomonas, Polaris, Dunaliella: Dunaliella, Prochlorococcus: Prochlorococcus, Sediococcus : Epicoccus, Volvox: Volvoxcarteri (Volvoxcarteri), species in Volvox; : Silk algae, Ulva genus: Ulva pore, Ulva reticulae, Ulva arseni, Ulva genus: Ulva spp., Fischerella genus, Cladophora genus: Cladophora japonica, Cladophora sarkovichae, Cladophora clumps , Pharocystis, Pharocystis genus: Facystis macrobass, Phalcystis spp., Banana genus: Banana spp., Pteridia genus: Pachyphyllum spp., Racemosa spp., Sawflyphyllum spp. Plumella genus, Saccharomyces genus: Pseudomonas chinensis, subtubular Saccharomyces spp., constricted Saccharomyces spp., long Saccharomyces spp., flat Saccharomyces spp.; Cleophyllum genus, Cleophyllum genus, Crescentella, Trichophyton, Cystoma, Tennis: Tennis, Reef: Reef membrane, Broad reef membrane, Grignard reef membrane, Thinaceae Algae genus, Coleopsis spp., Pyrophyllum genus, Orange Algae genus: Orange Algae, Double Star Algae genus, Aperia genus, etc.).

Cyanobacteria (Cryanobacteria: Suizenji Cyanobacteria, Microcystis, Nostoc: Nostoc schizophrenia, Nostoc vulgaris, Nostoc hairy, Oscillatoria, Spirulina: Spirulina, Trichophyton, etc. ).

Brown algae (Mesocystis genus: Mesocystis, Shuiyun genus: Indian water cloud, Saccharomyces genus: Saccharomyces spp., Phaeocystis spp.: Phaeocystis fungiformis, Blacktops genus: Tricornutum tricornutum, Thalerina genus: Filamentous Thaloptera, Verflagellate genus: Cylindrical Verflainae, Verflabina, Scattered Verflanophyllum, Reticulum genus: Reticulopsis, Broad-leaf Reticulum, Ceruleus genus: Ceruleus . Phaeopsis dendriticum, Padiophyllum macrophylla, Padiophyllum baumannii, Brachyphyton: Phaeopsis zonata, Phaeophthora: Suspicious brown hairy algae, Phaeopsis: Phaeopsis: Phaeopsis, Goldfish Algae genus: Chrysophyta, Cortella: Whistleella, Cladophylla: Cladophyllum Gangcun, Silkia: Silky, Noodles: Noodles, Coccidioides: Sclerophyllum, Black Algae genus: Hydra, Acremen: Apichotoma, Mozuku: Mozuku, Cinnamona: Cinnamona, Leafy Cinnamona, Prunus genus: Ceratophyllum, Trichocephala Genus: Trichocephala, Neptunium Genus: Tangled Neptunus, Acidella: Acidula lingulae, Acidella, Light brown Acidphylla, Achthonium: Smooth Achthonium, Phytophthora: Acidophyllum, Gooseflesh Entericia: goose sausage, Apatella genus: Apatella, Colepsia: Colepsia, Mesoporium: Japan Mesoporum, Cystis: Cystis, Longocystis, Thousand Island Cysticella: Thousand Island Cystis, Reticulopsis: Reticulocystis, Trichospora: Trichosporium, Cylinophyceae: Sclerocystis spp. Cyclocystis, Cyclocystis genus: Hypocystis spp., Reticula genus: Reticula spp., Cordella genus: Cordella spp., Porphyria spp.: Porphyrophyllum spp., Polyostella genus: Pleurophyllum spp. Laminaria japonica, Laminaria: Leathery kelp, Long handle kelp, Ghost kelp, Ezo kelp, Long kelp, Endo kelp, Laminaria cichorioides Miyabe, Laminaria cichorioides Miyabe, Laminaria cichorioides Miyabe, Pseudo-Laminaria: Pseudo-kelp, Laminaria Genus: Cave Kelp, Creeping Kelp, Kelp, Chrysanthemum : Escherichia two rounds, Pterosa genus: Elongatorium, Pachyptera, Carrageena: Carrageenia basilisk, Phyllocystis genus: Phyllocystis cuneiformis, Trumpetella genus: Trumpetella, Cysticella genus: Cystopella hakodate, Cystopella, Cystophyllumturneri Yendo, Sargassum genus: Sargassum nigrifolium, Sargassum segii Yoshida, Copperweed, Sargassum linearis, Sargassum nigrifolium, Sargassum nigrifolium Yendo, Sargassum sargassum, Sargassum tosa, Sargassum sargassum, Sargassum sargassum, Sargassum renneri, Artemisia japonica, Sargassum sargassum, Sargassum microspinosa, Sargassum split leaf, Sargassum datuo, Sargassum macrophyllum , Cyclococcus genus: Cyclococcus reinhardt, Macrophylla genus: Macroalgae, Coelocystis genus: Coelocystis genus, Hemerococcus genus: Hemerocystis, etc.).

Red algae (Rustella genus: dark purple red cabbage, small red cabbage, Porphyra: sweet laver, striped laver, quasi-linear laver, long laver, Tianzuo laver, multi-colored laver, broad laver, red line algae: Spinopsis huisneri, Filamentosa genus: silk slime, genus Adenodia: spp., japonica japonica, phalaenopsis, Hyssophyllum genus: hyssop, multi-row hyssop, fork Dendrhizomyceae, Dermides: Dermides, Forkdenedes, Pythias: Pyrethias, Pyrethia: Japanese Pyrethia, Pyrethium: Okamura Pyrethia, Trichodontia genus: Trichophyton fragilis, Drosophila genus: Cyclocystis spp., Dichromyces falciparum, Ptilonia genus: Ptilonia okadae, Ctenodonium genus: Pterodactylum genus, Hymenopsis genus: Eucalyptus eucalyptus Algae, Hydrangea macrophylla, Hydrangea: Microphyllum, Lycophyllum, Large Polyphylla, Amophyllum, Weak Gemini, Hydrangea (Hydrangea macrophylla), Whispered Gemini, Pleurophylla: Japanese softweed Dudresnaya minima, Dudresnaya minima, Dudresnaya minima: Dudresnaya minima, Piederia: Dudresnaya minima, Glissia: Thickened Gliss, Glissiana, Zentia : Constantinea genus, Constantinea genus: Constantinea subulifera, Neodilsea genus: Neodilsea yendoana, Neodilsea tenuipes, Molluscus genus: Sophia japonica , Softgrass japonica, Erythrodrichum genus: Erythrodon spp., Contarinia genus: Contarinia okamurae, Auricularia genus: Peyssonneliacaulifera Okamura, Auricularia genus: Auricularia japonica, Forknosia genus: Dictelopsis latissima Coral algae: coralline algae, centipede algae: Asian centipede algae, branched centipede algae, branched centipede algae, tongue-shaped centipede algae, Gangcun centipede algae, Fuwa centipede algae, fleshy centipede algae, oval centipede algae, belt Centipede algae, Centipede lanceolata, Centipede lanceolata, Halymenia: Slippery algae, Halymenia polydactyla, Centipede apicala, Serratia: Sawtoothia, Cypress: Hypress, Shield fruit Genus: hard scutellaria, stencilola, scutellum, multibranched scutellum, crepe scutellum, forked scutellum, sclerotium, horny scutipede, extremely high scutipede, branched Centipede, Cryptocystis: Schimmelmannia, Schimmelmannia: Schimmelmannia plumosa, Schimmelmannia: Schimmelmannia plumosa, Plumosa: Plumosa, Pygmy antler, Antler Plumosa, Pectopia: Peptoria , Beauty leaf algae genus: American leaf algae, Curly American leaf algae, Palmiform American leaf algae, Epiphytic American leaf algae, Adherent American leaf algae, Cockscomb American leaf algae, Callophyllis hayamensis, Purple American leaf algae, Beak head American leaf Algae, American leaf genus: American leaf algae, Aspergillus genus: Aspergillus spp., Kryptomyces genus: Sessioli spp., Kallym enia sagamiana, Kallymenia callophylloides, Smithia: Japanese Smithia, Tsengia lancifolia: Tsengia lancifolia, Tsengia: smooth algae, Schizophora: Schizophyllum, Thalassium: sea Netweed, Slippery genus: Yamada Slippery Algae, Red Leaf: Stout Red Leaf, Thin Red Leaf, Cockscomb: Felt-like Cockscomb, Turneria: Turneria, Strandella : Streptococcus clumps, Rhizoma genus: Rhizoma japonica, Cartilaginous Rhizoma japonica, Glycyrrhizae genus: Glycyrrhizae japonica, Glycyrrhiza japonica, Variation Glycyrrhizae japonica, Bacteria genus: Bacteria japonica , Gracilaria genus: Gracilaria genus, Gracilaria genus: Sea fungus, Gracilaria genus: Gracilaria true, Gracilaria rope, Gracilaria brittle, Gracilaria thick, Gracilaria inflexible, Gracilaria flat, Gracilaria caudate , Gracilaria constricta, Gracilaria star, Gracilaria subtidal, Gracilaria yamamoto, Gracilaria broom, anchovy, asparagus, Gracilaria variegata, Gracilaria bow, Gracilaria taro root, Gracilaria corymbosa, Gracilaria cuneiformis Gracilaria, Lithophyllum: Lithophyllum, Gracilaria: Gracilaria constricta, Phytophthora: Phytophthora, Pyromyces: Pyrethia, Pyrocladia: Pectinosa, Eyneum Genus: Igutina, Pleurotina glabra, Pseudomonas chinensis, Creekside fern: Creekside fern, Cirella genus: Sagittaria, Gigartina ochotensis, Pacific cypress, Ginkgo biloba genus: Blackleaf Ginkgo biloba, Rhodoglossus genus: Rhodoglossus japonicum, Glycotella spp.: Glychophylla japonicum, Netcystis spp.: Netcystis spinosa, Netcystis stipe, Chrysophylla: Chrysophysis, Okamura Aureum Algae, Coeloides: Coeloides, Brucetella, Botrytis: Botrytis, Cryptonia: Cryptonia, Palmitella: Palmate, Intricate Dulse, Lactus Dulse, Chrysostomum genus: Thalina glabra, Cysticula genus: Cysticocystis endodii, Arthropodium genus: Arthrophyllum catensis, Phyllospermum spp., Arthrophyllum spp. : Euphorbia fluorescens, Anophyllum extensa, Asparagus genus: Three-fork Xiancai, Soft Xiancai, Cone Xiancai, Japanese Xiancai, Borden Xiancai, Condensia genus: Uncarya, Condensia, Climberia genus : Softweed Climbingeria, Renbaoca: Renbaoshishi Shishi, Rhodophyceae: Margsonii Scheischii, Michelia Kurshima, Neophyllophyllum japonica, Cladophyllum genus: Cladophyllia creeping, Polyvena genus: Japan Polyphyllum, Neophyllum yezoense: Neophyllum yezoense, Myriogramme: Myriogramme polyneura, Hideophyllum yezoense, Acrophyllum: Acrophyllum yezoense, Acrophyllum yezoense, Acrome phyllum yezoense, Acrophyllum yezoense Algae, Hymenena genus: Hymenena tenuis, Hymenena genus: Rhodotrichum brittle, Partridge genus: Partridge without septum, Chrysomyces genus: Sessilis spp. Pseudomonas, Pseudotrichum: Pseudomonas pinnatifera, Polytubules: Intraclads, Pseudomonas brittle, Partridge: Partridge, Chondrophyta: Dendritic cartilage, thick branches Cartilaginous algae, Cartilaginous arbuscularis, Cartilaginous tangles, Cartilaginous ryukyuensis, Cartilaginous creeping, Cartilaginous microcartilaginous species, Dichonium sp. Duckweed, Duckweed, Pterophyta: Pterophyta, Pterophyta, Duckweed: Mossy Duckweed, Duckweed, Linear Duckweed, Climber: Amansia mitsuii Segawa, Amansia mitsuii Segawa, Amansia mitsuii Segawa, Amansia mitsuii Segawa, Amansia mitsuii Segawa, Amansia mitsuii Segawa, Leria genus: Laurel Pyromycetes, Pythia genus: Pythymella, Neoporphyra: Neopsiphyllum prickly, Dentalia: Odonthaliacorymbifera, Pyromycetes: Pyromonocystis, Pyrocystium, Black Pyrophyllia, Hot Spring Red Algae: Hot spring red algae, Pseudomonas: Pseudomonas, Polytubella, Porphyridium: Porphyridium, Rhodochrophyta: Rhodochrophyllum, etc.).

Tritium algae (Charophyta, Cleetidia, Pseudomonas: Pseudomonas obtuseum, Dimitrophyta, Cetella: Cetella flexorus, Cetella acupoints, Animonium, etc.), yellow algae (Chrysogenum genus: Chrysophylla rosenbergii, etc.) etc.

Examples of animal source materials include cockscomb extracts, bovine, porcine, or human placenta extracts, bovine or porcine stomach, duodenum, intestine, spleen extracts or their decomposed products, bovine or porcine brain tissue extracts , collagen hydrolyzate of bovine, porcine or fish (treated with acid, alkali, enzyme, etc.), water-soluble collagen and collagen derivatives such as acylated collagen; elastin or elastin hydrolyzate of bovine or porcine (acid, alkali, enzyme, etc.) and water-soluble elastin derivatives, keratin, its decomposed products and their derivatives, silk protein, its decomposed products and their derivatives, porcine or bovine blood cell protein decomposed products (globulin Peptides), bovine or porcine hemoglobin decomposition products (hemin, hemin, heme, heme, and heme iron), cow's milk, casein, its decomposition products and their derivatives, skim milk Powder, its decomposed products and their derivatives, lactoferrin and its decomposed products, egg components, fish meat decomposed products, and nucleic acid-related substances (for example, ribonucleic acid, deoxyribonucleic acid).

As materials of plant or animal origin, arbitrary parts, cells, tissues, organs, metabolites, etc. derived from genetically modified organisms and cell fusions can also be used. Furthermore, undifferentiated cell populations or differentiated cell populations obtained by culturing arbitrary parts, cells, tissues, organs, etc., for example, can also be used.

(14) Marine ingredients

Examples of marine components include seawater, such as deep seawater, for example, sea salt and seawater dry matter; inorganic salts (for example, sodium chloride, magnesium chloride, and potassium chloride) obtained from seawater in the Dead Sea, the Atlantic Ocean or the Pacific Ocean, marine Muds and fangoes, such as Italian mud, German mud, Eifel mud, and Freiburg mud (ingredients: silicon dioxide, titanium dioxide, aluminum oxide, iron oxide, manganese oxide, oxide sodium, potassium oxide, magnesium oxide, calcium oxide, strontium oxide, sodium, potassium, magnesium, calcium, chromium, iron, copper, nickel, zinc, lead, manganese, arsenic and water), and santoku stone.

(15) Microbial culture metabolites

Examples of microbial culture metabolites include yeast metabolites, yeast extracts, bacterial metabolites, bacterial extracts, fungal and actinomycete metabolites, fungal and actinomycete metabolites, Bacillus natto metabolites, fermented soybean extracts Fermented rice extract, fermented rice bran (red bran, white bran) extract, euglena extract, its decomposed products and their water-soluble derivatives, trehalose and its derivatives, raw milk and skimmed milk powder lactic acid fermentation , lactic acid bacteria fermented products of leguminous plants, and lactic acid bacterial fermented products of coconut plants.

(16) α-hydroxy acid

Examples of alpha-hydroxy acids include glycolic acid, citric acid, malic acid, tartaric acid and lactic acid.

(17) Inorganic pigments

Examples of inorganic pigments include silicic anhydride, magnesium silicate, talc, kaolin, bentonite, mica, mica titanium, bismuth oxychloride, zirconia, magnesium oxide, zinc oxide, titanium oxide, calcium carbonate, magnesium carbonate, iron oxide yellow, Iron oxide red, iron oxide black, ultramarine blue, chromium oxide, chromium hydroxide, carbon black, and hydrozincite.

(18) UV absorbers and blockers

Examples of UV absorbing and blocking agents include benzophenone derivatives (e.g., 2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid , 2-Hydroxy-4-methoxybenzophenone-5-sodium sulfonate, dihydroxydimethoxybenzophenone, dihydroxydimethoxybenzophenone-sodium sulfonate, 2,4 -dihydroxybenzophenone, and tetrahydroxybenzophenone), p-aminobenzoic acid derivatives (for example, p-aminobenzoic acid, ethyl p-aminobenzoate, glyceryl p-aminobenzoate, amyl p-aminobenzoate esters, octyl p-aminobenzoate), methoxycinnamic acid derivatives (for example, ethyl p-methoxycinnamate, isopropyl p-methoxycinnamate, octyl p-methoxycinnamate, p-methoxycinnamate 2-ethoxyethyl cinnamate, sodium p-methoxycinnamate, potassium p-methoxycinnamate, and glycerol di-p-methoxycinnamate mono-2-ethylhexanoate), salicylic acid derivative substances (eg, octyl salicylate, phenyl salicylate, high ester, dipropylene glycol salicylate, ethylene glycol salicylate, myristyl salicylate, and methyl salicylate), anthranilic acid derivatives (for example, methyl anthranilate, etc.), Urocanic acid derivatives (for example, urocanic acid and urocanic acid ethyl ester), coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, di Alkane derivatives, camphor derivatives, furan derivatives, pyrone derivatives, nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives, vitamin B6 derivatives, umbelliferone, aescin, benzyl cinnamate , xinuosi, oxybenzone, dioxybenzone, octylbenzone, sulisobenzone (sulisobenzone), benzoyl resorcinol, arbutin, guaiazulene, shikoning, baicalin ), baicalein, berberine, Neo Heliopan, Escalol, zinc oxide, talc, and kaolin.

(19)Skin whitening agent

Examples of skin whitening agents include p-aminobenzoic acid derivatives, salicylic acid derivatives, anthranilic acid derivatives, coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, substances, pyrimidine derivatives, two Alkanes derivatives, camphor derivatives, furan derivatives, pyrone derivatives, nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives, vitamin C and its derivatives (for example, vitamin C phosphate magnesium salt and vitamin C glucoside), vitamin E and its derivatives, kojic acid and its derivatives, oxybenzone, benzophenone, arbutin, guaiazulene, shikonin, baicalein, baicalein, berberine , placenta extract, ellagic acid, and resveratrol (Rucinol).

(20) Tyrosinase activity inhibitors

Examples of tyrosinase activity inhibitors include vitamin C and its derivatives (e.g., ascotyl phosphate magnesium salt and ascorbic acid glucoside), hydroquinone and its derivatives (e.g., hydroquinone benzyl ether ), kojic acid and its derivatives, vitamin E and its derivatives, N-acetyltyrosine and its derivatives, glutathione, hydrogen peroxide, zinc peroxide, placenta extract, ellagic acid, bearberry Glycosides, resveratrol, silk extracts, and plant extracts (for example, chamomile, mulberry, common gardenia, Japanese angelica, Burnet, flavescens, mugwort, Japanese honeysuckle, cork bark, Houttuynia cordata, Poria cocos, Coix, wild sesame, hops, Japanese hawthorn, eucalyptus, yarrow, hollyhock, cinnamon, vitex, witch hazel, chicken mulberry, calamus, platycodon, dodder, capers, shegan, ephedra, chuanxiong, lovage, Bupleurum, Parsnip, Coral Vegetable, Skullcap, Moutan Bark, Giraffe, Pueraria Root, Licorice Root, Gallnut, Aloe Vera, Cimicifuga Root, Safflower, Green Tea, Black Tea, and Immortality).

(21)Melanin pigment reduction/decomposition substances

Examples of melanin pigment reducing/decomposing substances include phenylmercuric hexachlorophene, mercuric oxide, calomel, hydrogen peroxide, zinc peroxide, hydroquinone and its derivatives (e.g., hydroquinone benzyl ether ).

(22) Substances that promote metabolic turnover/cell activation

Examples of substances that promote metabolic turnover/cell activation include hydroquinone, lactic acid bacteria extract, placenta extract, ganoderma lucidum extract, vitamin A, vitamin E, allantoin, spleen extract, thymus extract, yeast extract , fermented milk extracts, and plant extracts (for example, aloe vera, baicalin, horsetail, gentian, burdock, purple root, carrot, witch hazel, hubu, coix seed, wild sesame, Dangyao, Dongying Angelica, calendula flowers, sweet tea, forsythia, cucumber, thyme, rosemary, and coriander).

(23) Astringent

Examples of astringents include succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, zinc hydrochloride, zinc p-phenolsulfonate, potassium aluminum sulfate, resorcinol, ferric chloride, and tannins (including catechin compounds).

(24)Active oxygen scavenger

Examples of active oxygen scavengers include SOD, catalase, and glutathione peroxidase.

(25)Antioxidant

Examples of antioxidants include vitamin C and its salts, stearate, vitamin E and its salts, nordihydroguaiaretic acid, butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), hydroxy Tyrosol, paraben, propyl gallate, sesamol, sesamolin, gossypol, and propolis.

(26) Lipid peroxidation production inhibitor

Examples of lipid peroxidative production inhibitors include β-carotene and plant extracts (e.g., sesame cultured cells, ganja leaves, forsythia, witch hazel, clove, melissa officinalis, sativa, birch, sage, Rosemary, Nantian Fruit, Nutmeg, Ginkgo, and Green Tea).

(27) Anti-inflammatory agent

Examples of anti-inflammatory agents include ichthyol, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d-camphor, dl-camphor, hydrogenated Cortisone, Guaiazulene, Chamazulene, Chlorpheniramine Maleate, Glycyrrhizinic Acid and Its Salts, Glycyrrhetinic Acid and Its Salts, Licorice Root Extract, Purple Root Extract, Nutritious Fruit Extract, and Propolis.

(28) Antimicrobials, fungicides and disinfectants

Examples of antimicrobials, bactericides, and disinfectants include rivanol, sulfur, calcium gluconate, chlorhexidine gluconate, sulfamic acid, merbromin, lactoferrin and its hydrolyzate, alkane chloride Diaminoethylglycine solution, triclosan, sodium hypochlorite, chloramine T, bleaching powder, iodine compounds, iodoform, sorbic acid and its salts, propionic acid and its salts, salicylic acid, dehydroacetic acid, p-hydroxybenzoic acid Esters, Undecylenic Acid, Thiamine Lauryl Sulfate, Thiamine Lauryl Nitrate, Phenol, Cresol, Para-Chlorophenol, Para-Chloro-Meta-Xylenol, Para-Chloro-Meta-Cresol, Thymol , Phenylalcohol, o-Phenylphenol, Irgasan CH3565, Halocarban, Hexachlorophene, Chlorhexidine, Ethanol, Methanol, Isopropanol, Benzyl Alcohol, Ethylene Glycol, Propylene Glycol, 2-Phenoxyethanol , 1,2-pentanediol, zinc pyrithione, chlorobutanol, isopropylmethylphenol, nonionic surfactants (for example, polyoxyethylene lauryl ether, polyoxyethylene nonylphenyl ether, and polyoxyethylene octylphenyl ether), amphoteric surfactants, anionic surfactants (for example, sodium lauryl sulfate, and potassium lauroyl sarcosinate), cationic surfactants (for example, cetyltrimethyl bromide Ammonium, Alkylbenzyldimethylammonium Chloride, Benzethonium Chloride, and Methylrosaniline Chloride), Formaldehyde, Hexamethylenetetramine, Brilliant Green, Malachite Green, Crystal Violet, Germall, Photosensitive 101 No., photosensitive element 201, photosensitive element 401, N-long-chain acyl basic amino acid derivatives and their acid addition salts, zinc oxide, 4-isopropyl cycloheptadienolone, flavescens, and Propolis.

(29) Moisturizer

Examples of humectants include glycerin, propylene glycol, 1,3-butanediol, hyaluronic acid and its salts, polyethylene glycol, chondroitin sulfate and its salts, water-soluble chitin derivatives, water-soluble chitosan derivatives , pyrrolidone carboxylic acid and its salt, sodium lactate, Mini Sasanishiki extract, Bacillus natto metabolite, fermented soybean extract, loofah water, and birch sap and Japanese red pine sap.

(30) Elastase Activity Inhibitors

Examples of inhibitors of elastase activity include diisopropylfluorophosphate, plant extracts (e.g., skullcap, forsythia, flavescens, mulberry leaves, cinnamon bark, gerania, comfrey, sage, elderberry , linden tree, and peony bark), and seaweed extract.

(31) Hair care agents

Examples of hair care agents include selenium disulfide, alkylisoquinolinium bromide, zinc pyrithione, benzalamide ester, dimethylthioanthrene, beaver tincture, ginger tincture, capsicum tincture, quinine hydrochloride , Strong Ammonia, Potassium Bromate, Sodium Bromate, and Thioglycolic Acid.

(32) Antiandrogens

Examples of antiandrogens include estrogens (eg, estrone, estradiol, and ethinyl estradiol), isoflavones, and isoethinorone.

(33) Peripheral blood flow promoting agent

Examples of agents for promoting peripheral blood flow include vitamin E and its derivatives, herb extracts, garlic extracts, ginseng extracts, aloe extracts, gentian extracts, angelica extracts, acresia tetrandrine, carprochloramine , Hemin Leding.

(34) Stimulants

Examples of stimulants include capsicum tincture, nonanoic acid vanillylamide, cantharidin tincture, ginger tincture, peppermint oil, 1-menthol, camphor, benzyl nicotinate, and the like.

(35) Metabolic Active Agents

Examples of metabolically active agents include photosensitive 301, 4-isopropylcycloheptadienolone, pantothenic acid and its derivatives, allantoin, placental extract, biotin, and glyceryl pentadecanoate.

(36) Anti-seborrheic agent

Examples of anti-seborrheic agents include pyridoxine and its derivatives, sulfur, and vitamin B6.

(37) Keratolytics

Examples of keratolytic agents include resorcinol, salicylic acid, lactic acid, and urea.

(38)Oxidizing agent

Examples of oxidizing agents include aqueous hydrogen peroxide, sodium persulfate, ammonium persulfate, sodium perborate, urea peroxide, sodium percarbonate, sodium tripolyphosphate peroxide, sodium bromate, potassium bromate, sodium pyrophosphate peroxide, peroxide Sodium orthophosphate, sodium silicate hydroperoxide, sodium sulfate hydroperoxide, sodium chloride hydroperoxide, beta-tyrosinase solution, and mushroom extract.

(39) Hair remover

Examples of depilatory agents include inorganic reducing agents such as strontium sulfate, sodium sulfide, barium sulfide, and calcium sulfide; thioglycolic acid and salts thereof (such as calcium thioglycolate, sodium thioglycolate, lithium thioglycolate, magnesium thioglycolate, and strontium thioglycolate) .

(40) Hair conditioner

Examples of hair swelling agents include ethanolamine, urea, and guanidine.

(41) Dyes

Examples of dyes include 5-aminoo-cresol, 2-amino-4-nitrophenol, 2-amino-5-nitrophenol, 1-amino-4-methylaminoanthraquinone, 3,3'- Aminodiphenol, 2,4-diaminophenoxyethanol hydrochloride, 2,4-diaminophenol hydrochloride, toluene-2,5-diamine hydrochloride, nitro-p-phenylenediamine hydrochloride, p-phenylenediamine hydrochloride, hydrochloric acid N-phenylenediamine, m-phenylenediamine hydrochloride, o-aminophenol, N-phenylenediamine acetate, 1,4-diaminoanthraquinone, 2,6-diaminopyridine, 1,5- Dihydroxynaphthalene, toluene-2,5-diamine, toluene-3,4-diamine, nitro-p-phenylenediamine, p-aminophenol, p-nitro-o-phenylenediamine, p-phenylenediamine, p-methylamino Phenol, picric acid, sodium picric acid, N,N'-bis(4-aminophenyl)-2,5-diamino-1,4-quinonediimine, 5-(2-hydroxyethylamino )-2-methylphenol, N-phenyl-p-phenylenediamine, m-aminophenol, m-phenylenediamine, 5-amino-o-cresol sulfate, 2-amino-5-nitrophenol sulfate, o-aminophenol sulfate, o-chloro-p-phenylenediamine sulfate, 4,4'-diaminodiphenylamine sulfate, 2,4-diaminophenol sulfate, toluene-2,5-diamine sulfate, nitro-p-phenylenediamine sulfate, p-phenylene sulfate Aminophenol, p-nitro-o-phenylenediamine sulfate, p-nitro-m-phenylenediamine sulfate, p-phenylenediamine sulfate, p-methylaminophenol sulfate, m-aminophenol sulfate, m-phenylenediamine sulfate, catechol, di Phenylamine, α-naphthol, hydroquinone, pyrogallol, phloroglucinol, gallic acid, resorcinol, tannic acid, 2-hydroxy-5-nitro-2',4'-diamino Sodium azobenzene-5'-sulfonate, and hematine.

(42) Spices

Examples of fragrances include natural animal fragrances such as musk, civet, castoreum, and ambergris; plant fragrances such as anise essential oil, angelica essential oil, ylang ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, perfume tree essential oil, caraway Essential oils, cardamom essential oil, guaiac wood essential oil, cumin essential oil, camphor essential oil, cinnamon essential oil, geranium cinnamon essential oil, geranium essential oil, Cuban balsam essential oil, coriander essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, Jasmine essential oil, ginger grass essential oil, Japanese fir essential oil, spearmint essential oil, peppermint essential oil, anise essential oil, tuberose essential oil, clove essential oil, neroli essential oil, wintergreen essential oil, tolu balsam essential oil, sansevieria essential oil, rose rose essential oil , Palmarosa essential oil, Japanese cypress essential oil, Rohan cypress essential oil, white sandalwood essential oil, lime essential oil, geranium essential oil, vetiver essential oil, citrus essential oil, Peruvian balsam essential oil, rosewood essential oil, cinnamon essential oil, citrus essential oil, eucalyptus essential oil , Lime Essential Oil, Lavender Essential Oil, Agarwood Essential Oil, Lemongrass Essential Oil, Lemon Essential Oil, Rosemary Essential Oil, Japanese Peppermint Essential Oil; and other synthetic fragrances.

(43) Pigments and colorants

Examples of pigments and coloring agents include red cabbage pigment, red rice pigment, madder pigment, annatto pigment, squid melanin, curcumin pigment, sophora pigment, krill pigment, persimmon pigment, caramel, gold, silver, gardenia Color, corn color, onion color, tamarind color, spirulina color, buckwheat whole grass color, cherry color, seaweed color, hibiscus color, grape juice color, calendula color, purple sweet potato color, purple yam color, shellac color , and rutin.

(44)Other additives

Examples of other additives include hormones, metal ion blocking agents, pH regulators, chelating agents, antiseptic/antifungal agents, cooling agents, stabilizers, emulsifiers, animal/vegetable proteins and their decomposition products, animal/vegetable Polysaccharides and their decomposition products, animal/vegetable glycoproteins and their decomposition products, blood flow promoting agents, anti-inflammatory agents/anti-inflammatory agents, anti-allergic agents, wound healing agents, foam enhancers, thickeners, enzymes, purification Water (including electronic water, small cluster water, etc.), oral preparations, deodorizing/deodorant agents, bittering agents, seasonings, etc. Use of these additives in combination can be expected to additively or synergistically improve the effect of the present invention or other cosmetic effects.

Example

Examples are described in which Enterococcus faecalis EC-12, which is the most prominent among the target effects of the present invention, is used as a lactic acid bacterium of the genus Enterococcus, but the present invention is not limited to the following examples and the like.

(preservation of microorganisms)

On February 25, 2005 (the original deposit date), Enterococcus faecalis EC-12 was deposited in the National Institute of Advanced Industrial Science and Technology, the International Patent Biological Deposit Center (Central No. 1, East 1-chome, 1, Tsukuba, Ibaraki Prefecture, Japan, AIST ). The deposit number is FERM BP-10284.

According to a preferred embodiment of the present invention, the bacteria used as the active ingredient are bacteria belonging to the genus Enterococcus. More preferably the bacterium is Enterococcus faecalis.

Examples of Enterococcus faecalis include strains such as Enterococcus faecalis EC-12 (Accession No. FERM BP-10284), ATCC 19433, ATCC 14508, ATCC 23655, IFO 16803, and IFO 16804, and variants thereof. As bacteria used as an active ingredient, among them, the EC-12 strain is most preferable.

The term "mutant strain" as used herein is intended to include, for a specific strain, a strain obtained by a person skilled in the art by mutating it within a range that does not change its properties by a method well known in the art, and a strain obtained by those skilled in the art. A human-identifiable equivalent strain.

(Lactic acid bacteria of the genus Enterococcus and the acquisition of heat-killed cells)

Enterococcus faecalis EC-12 (Accession No. FERM BP-10284) purchased from Combi Co., Ltd. was used.

(production of plant polysaccharides)

Aloe vera polysaccharides were produced as plant polysaccharides. After 100 g of Aloe vera leaves were pulverized, squeezed, extracted and filtered, ethanol or isopropanol was added to the obtained filtrate, followed by stirring. Then, the produced precipitate was recovered, and after adding ethanol or isopropanol to wash again, the obtained washed precipitate was dried to obtain Aloe vera polysaccharide powder.

(Preparation of samples)

Heat-killed cells of lactic acid bacteria of the Enterococcus genus produced above were mixed with Aloe vera polysaccharide to prepare samples 1 to 4 shown in Table 1 (final concentrations added to the culture medium).

[Table 1]

sample 1 2 3 4 EC-12 heat kills cells 0% 1.0% 0% 1.0% Curacao Aloe Vera Polysaccharide 0% 0% 0.1% 0.1%

(Culture of Staphylococcus epidermidis model bacteria)

Nutrient Broth No. 2 (manufactured by Nissui Pharmaceutical Co., Ltd.) was used as a medium. Samples 1-4 were added to this medium (values in the table: final concentration in the medium, %). Then, the culture medium was inoculated with Staphylococcus epidermidis model bacteria (ATCC12228) so that 1 to 5×10 ³ CFU/μL was obtained, and cultured at 37° C. for 24 hours. As a control, a sample (sample 1) to which no lactic acid bacteria of the Enterococcus genus had been heat-killed was used.

(Collection method of human skin resident bacteria)

Use a sterilized cotton swab to wipe the glabella area of 3 cm ² three times vertically and three times horizontally to collect skin resident bacteria. Subsequently, the cotton swab was washed in 10 mL of phosphate-buffered saline to collect bacteria.

(Culture of human skin resident bacteria)

Nutrient Broth No. 2 (manufactured by Nissui Pharmaceutical Co., Ltd.) was used as a medium. Lactic acid bacteria belonging to the Enterococcus genus were added to this medium so that the final concentrations were 0.25, 0.5, and 1.0 w/w%, and the cells were heat-killed. Then, 100 µL of each medium was inoculated with the skin-resident bacteria collected by the above method, which were then cultured at 37°C for 24 hours. A medium to which no heat-killed bacterial cells was added was used as a control.

(Measurement of Glycerin Amount)

Separate the culture solutions obtained from the above-mentioned Staphylococcus epidermidis model bacteria (ATCC12228) and human skin resident bacteria. These culture solutions were stirred with a vortex mixer, and the amount of glycerol was measured according to a conventional method using a glycerin detection kit (manufactured by Cayman Chemical).

(Measurement result of the amount of glycerin)

The results are shown in Figures 1 and 2. Addition of heat-killed cells of Enterococcus lactic acid bacteria resulted in a significant increase in glycerol production by the S. epidermidis model strain (ATCC 12228) ( FIG. 1 ). When the final addition concentration in the medium was 1.0 w/w% (Sample 2), about 10-fold effect of promoting glycerol production was observed. Further, when added in combination with Aloe vera polysaccharide (0.1 w/w%) (Sample 4), the glycerol production of the Staphylococcus epidermidis model bacteria was synergistically increased. An increase in the amount of glycerol production was also observed in the culture solution of human skin-resident bacteria by adding heat-killed cells of Enterococcus lactic acid bacteria ( FIG. 2 ).

(Human Subject Trial)

The male and female team members (18 subjects in total) applied a softening lotion (lotion A) containing heat-killed cells of Enterococcus lactic acid bacteria on the right cheek after washing their faces before going to bed every day, and a control lotion on the left cheek. Placebo soft lotion (lotion B) that does not contain heat-killed cells of Enterococcus lactic acid bacteria, used continuously for 7 days (the formula of these soft lotions is as follows), after getting up on the 8th day, use a sterilized cotton swab to follow the vertical direction of 3 Wipe each cheek with an area of 3 cm ¹ time and 3 times laterally to collect glycerin on the skin surface. Then, wash the swab in 200 μL of phosphate-buffered saline to disperse metabolites.

(soft lotion)

The above components were uniformly mixed according to a conventional method to obtain a soft lotion (lotion A and lotion B).

(Measurement of Glycerin Amount)

The dispersion liquid obtained above was stirred with a vortex mixer, and the amount of glycerin was measured according to a conventional method using a glycerin detection kit (manufactured by Cayman Chemical).

(Measurement result of the amount of glycerin)

The results are shown in Figure 3. The use of a softening lotion containing heat-killed cells of Enterococcus lactic acid bacteria resulted in a significant increase in the amount of glycerol on the skin surface of human subjects compared to the use of a control softening lotion.

(Sensory Evaluation in Human Subject Tests)

Questionnaire surveys were conducted on the feeling of using the soft lotion and the skin condition before and after the test to the male and female team members (18 subjects in total) participating in the human monitor test. The results of the answers are shown in Table 2.

[Table 2]

(Quantitative analysis of human β-defensin-2 (hBD2), human β-defensin-3 (hBD3) mRNA)

In a 24-well culture plate, human epidermal keratinocytes (manufactured by Kurabo Industries Co., Ltd.) were cultured to a sub-confluent state using a medium for epidermal cells (Humedia KG2, produced by Kurabo Industries Co., Ltd.). After human epidermal keratinocytes were further cultured in a differentiation-inducing medium prepared by adding calcium to Humedia KG2 at 37° C. in the presence of 5% CO ₂ for 3 days, the medium was replaced with a fresh medium. Lactic acid bacteria belonging to the Enterococcus genus were added to the medium at final concentrations of 0.25, 0.5, and 1.0 w/w%, and the cells were heat-killed. The model bacteria Staphylococcus epidermidis was added at a concentration of about 1×10 ⁴ cells/mL as a positive control. After culturing for 6 hours, total RNA was extracted from human epidermal keratinocytes using RNeasy (manufactured by Qiagen). Using each extracted RNA as a template, cDNA was prepared by performing a reverse transcription reaction using SuperScript VILO (manufactured by Invitrogen) by a conventional method.

PCR was performed using the cDNA thus obtained. As for PCR conditions, based on the cDNA sequence of human β-defensin-2 (Accession No. NG_023301) and human β-defensin-3 cDNA sequence (Accession No. NC_000008), the primers shown in Table 3 were prepared and used TAKARA Human Housekeeping Gene Primer Set Human ACTB Primer was used as a primer for β-actin used as an internal standard.

[table 3]

PCR was performed using One Step SYBRR PrimeScript ^™ RT-PCR Kit (manufactured by TAKARA Bio Inc.), and real-time PCR analysis was performed by a conventional method using TaKaRa PCR Thermal Cycler Dice. Relative quantification of mRNA amounts of human β-defensin-2, human β-defensin-3 and β-actin was performed based on real-time PCR analysis.

(Evaluation method of mRNA levels of human β-defensin-2, human β-defensin-3 and β-actin)

The mRNA amounts of human β-defensin-2, human β-defensin-3, and β-actin were evaluated as percentages relative to the mRNA amount of β-actin as an internal standard.

The relative expression level of human β-defensin-2 or human β-defensin-3 mRNA (% relative to the control)=(the amount of human β-defensin-2 or human β-defensin-3 mRNA)/(β-defensin-3 mRNA) Amount of actin mRNA)×100

(Results of quantitative analysis of human β-defensin-2 and human β-defensin-3 mRNA)

The results are shown in Figure 4. Addition of heat-killed cells of Enterococcus lactic acid bacteria also resulted in increased expression levels of β-defensin-2 and β-defensin-3 mRNA in human epidermal keratinocytes ( FIG. 4 ).

(Manufacture examples of various external preparations for skin protection)

1. Sunscreen cream (O/W cream type)

(oil phase)

(water box)

(Manufacturing method)

The oil phase and the water phase were heated to 70°C separately to dissolve the components. Titanium dioxide was sufficiently dispersed in the water phase, and an oil phase was added thereto, and after making the emulsified particles uniform using a homomixer, the resultant was degassed, filtered, cooled, and then accommodated. As a cooling method, for example, a method of slowly cooling the resultant while stirring while injecting a refrigerant into the jacket of the production tank, or a method of cooling using a heat exchanger is implemented.

2. Sunscreen lotion (W/O type lotion)

(oil phase)

(water box)

Purified water balance to 100

1,3-Butanediol 5.0%

(Manufacturing method)

Heat the oil phase and the water phase to 60°C respectively to dissolve the components, sufficiently disperse titanium dioxide and zinc oxide in the oil phase, add the water phase to it, and use a homomixer to make the emulsified particles uniform. The resultant was degassed, filtered, cooled and then housed.

3. Sunscreen

(Manufacturing method)

The silicone resin is added to the silicone oil and dissolved. Then, after adding isopropyl myristate and stirring well to mix, the remaining components were added and the resultant was stirred.

4. Powder foundation

(powder)

(Substrate)

Squalane 3.0%

Octyldodecyl myristate 3.5%

Vaseline 2.5%

(other components)

Appropriate amount of preservatives

Appropriate amount of spices

(Manufacturing method)

The powder components are mixed using a mixer such as a high-speed flow type mixer, and further made into fine particles using a pulverizer. The base material is then added to the fine particles, and thoroughly mixed using a vertical screw type mixer or the like. The resultant is further pulverized and granulated using a hammer mill or the like, and then compression-molded. 5. Dual-purpose foundation

(powder)

(Substrate)

(other components)

Appropriate amount of preservatives

Appropriate amount of spices

6. Cream (W/O type)

(Phase A)

(phase B)

Titanium Dioxide, Magnesium Stearate, Talc, Black Iron Oxide, Aluminum Hydroxide, Mica, Stearic Acid 1.13%

Red and brown iron oxide, magnesium stearate, titanium dioxide, mica, water, aluminum oxide, stearic acid 0.85%

Titanium Dioxide, Talc, Magnesium Stearate, Aluminum Hydroxide, Mica, Stearic Acid 3.9%

(phase C)

(D phase)

Phenoxyethanol 0.4%

(Phase E)

Titanium Dioxide, Glycerin, Aluminum Oxide 3.5%

(F phase)

Silica Dimethylsilylated 1.0%

(G phase)

Cyclopentasiloxane, (Dimethicone/Vinyltrimethylsiloxysilicate) 1.0%

cross-linked polymer

Heat-killed cells of Enterococcus lactic acid bacteria 0.01-1 (appropriate amount)

(H phase)

Fragrance 0.3%

(Manufacturing method)

Mix the raw materials of phase A with stirring and heat to 70-75°C. Then, add phase B to phase A with stirring and stir the result until homogeneous. Mix the raw materials of phase C in another container under stirring, and heat to 70-75°C, then slowly add phase C to the mixed phase A-B under stirring (about 5 minutes), add phase C and stir the resultant 3 minutes, and then cooled naturally. When the temperature of the resultant has cooled below 40°C, add phase D and stir for about 1 minute, then add phase E and stir for about 5 minutes, then add phase F and stir for about 1 minute, then add phase G and stir for about 1 minute minute. Phase H was finally added and stirred for about 2 minutes, after which the result was allowed to cool naturally to room temperature.

7. Moisturizing gel (beauty essence)

(Phase A)

Olive Oil Fatty Cetyl/Stearyl Ester + Sorbica 3.5%

Alcohol Olive Oil Fatty Acid Ester

(phase B)

(phase C)

(Manufacturing method)

Mix the respective materials of Phase A and Phase B separately. Xanthan gum and sodium polyacrylate are dispersed using a batch type disperser or the like, and phase A is added to phase B at 75°C. The resultant was homogenized for a few minutes, cooled with slow stirring, and phase C (menthol predissolved in ethanol previously dissolved) was added to it once the temperature had dropped to 40°C.

8. Lipstick

(Phase A)

(phase B)

Glyceryl tri(caprylic/capric) balance to 100

Ethylhexyl Methoxycinnamate 3.0%

Isopropyl myristate 10.0%

(phase C)

Alkyl (C26-28) methicone 9.0%

Cetyl Alcohol 1.6%

(D phase)

Blend of PEG-8, Tocopherol, Ascorbyl Palmitate, Ascorbic Acid and Citric Acid 0.1%

Propylparaben 0.1%

(Phase E)

(Manufacturing method)

Premix phase A evenly, then mix evenly with phase B. Phase C is further added thereto, and after heating the resultant to 78-80° C. under gentle stirring, phase D and phase E are added little by little thereto, and the resultant is stirred uniformly and poured into a mold, and then Let it cool down to room temperature naturally.

9. Liquid foundation (O/W type)

(oil phase)

(water box)

(powder)

(other components)

Appropriate amount of spices

(Manufacturing method)

The powder is pulverized and then mixed with the water phase. To this mixture, an oil phase and a fragrance are added with stirring, and then emulsified and dispersed with a homomixer, and the resultant is cooled to room temperature.

10. Cream foundation (W/O type)

(oil phase)

(water box)

Purified water balance to 100

Glycerin 5.0%

Appropriate amount of water-soluble preservative

(powder)

(other components)

Appropriate amount of spices

11. Moisturizing cream (O/W type)

(Phase A)

(phase B)

(phase C)

Spice 0.5%

Phenoxyethanol, Methylparaben, Paraben 0.5%

Ethyl, Butylparaben, Propylparaben,

Mixture with isobutylparaben

Tetrahydroxypropylethylenediamine 0.7%

(Manufacturing method)

After heating Phase A and Phase B to 70°C in separate containers, Phase A was added to Phase B, and the resulting mixture was homogenized using a batch type disperser or the like, and then naturally cooled to 40°C while stirring. Then, phase C was added to the resulting mixture, and the resultant was further homogenized and cooled to room temperature.

[Industrial availability]

The lactic acid bacteria of the genus Enterococcus of the present invention promote the production of glycerol derived from Staphylococcus epidermidis, which is a skin-resident bacterium beneficial to the skin, and promote the production of antimicrobial peptides derived from keratinocytes of the skin epidermis. Moreover, the Enterococcus lactic acid bacteria of the present invention give a very excellent silky feeling when applied to the skin, and can impart good touch to any water-type, oil-type, emulsion-type and powder-type external preparations. Therefore, the lactic acid bacteria of the Enterococcus genus of the present invention are ideally applicable to skin protection agents and external preparations for normalizing the skin condition or maintaining the normality of the skin.

Claims (12)

CLAIMS 1. A glycerol production accelerator derived from Staphylococcus epidermidis, the accelerator comprising lactic acid bacteria belonging to the genus Enterococcus as an active ingredient. 2. The glycerin production promoter derived from Staphylococcus epidermidis according to claim 1, wherein the lactic acid bacterium belonging to the genus Enterococcus is Enterococcus faecalis. 3. The glycerol production accelerator derived from Staphylococcus epidermidis according to claim 1 or 2, wherein the lactic acid bacterium belonging to the genus Enterococcus is Enterococcus faecalis EC-12 (Accession No. FERM BP-10284). 4. The glycerin production promoter derived from Staphylococcus epidermidis according to any one of claims 1 to 3, wherein the lactic acid bacteria belonging to the genus Enterococcus are killed. 5. The glycerin production promoter derived from Staphylococcus epidermidis according to any one of claims 1 to 4, wherein the lactic acid bacteria belonging to the genus Enterococcus are heat-killed. 6. An antimicrobial peptide production accelerator derived from skin epidermal keratinocytes, the accelerator comprising lactic acid bacteria belonging to the genus Enterococcus as an active ingredient. 7. The skin epidermal keratinocyte-derived antimicrobial peptide production accelerator according to claim 6, wherein the lactic acid bacterium belonging to the genus Enterococcus is Enterococcus faecalis. 8. The antimicrobial peptide production accelerator derived from skin epidermal keratinocytes according to claim 6 or 7, wherein the lactic acid bacteria belonging to the genus Enterococcus is Enterococcus faecalis EC-12 (Accession No. FERM BP-10284 ). 9. The skin epidermal keratinocyte-derived antimicrobial peptide production accelerator according to any one of claims 6 to 8, wherein the lactic acid bacteria belonging to the genus Enterococcus are killed. 10. The skin epidermal keratinocyte-derived antimicrobial peptide production accelerator according to any one of claims 6 to 9, wherein the lactic acid bacteria belonging to the genus Enterococcus are heat-killed. 11. The antimicrobial peptide production accelerator according to any one of claims 6 to 10, wherein the antimicrobial peptide is human β-defensin-2 (hBD2) or human β-defensin-3 (hBD3) . 12. An external preparation for skin protection comprising the glycerin production promoter derived from Staphylococcus epidermidis or the antimicrobial peptide production promoter derived from skin epidermal keratinocytes according to any one of claims 1 to 11.