CN118236306B - Mugwort leaf extract and application thereof in cosmetics - Google Patents

Abstract

The invention belongs to the technical field of daily chemicals, and particularly relates to a mugwort leaf extract and application thereof in cosmetics. According to the invention, the mugwort, the sage, the lavender and the neroli are crushed by adding water, then the ethanol aqueous solution is used for leaching, the extractant obtained by matching the oil phase consisting of the ethyl acetate and the cyclohexane with the water phase is used for extracting, and then the aftertreatment is carried out, so that the oily active ingredients of the mugwort, the sage, the lavender and the neroli, such as essential oil and the like, and the organic ingredients and the like can be effectively extracted, and the extract is endowed with excellent antioxidant and moisturizing effects.

Description

A kind of wormwood leaf extract and its application in cosmetics

Technical Field

The invention belongs to the technical field of daily chemical products, and specifically relates to an wormwood leaf extract and application thereof in cosmetics.

Background technique

Cosmetics are daily consumer goods that meet people's needs for "beauty" and have become an indispensable daily necessity in life. Cosmetics are daily chemical industrial products that are applied to the skin, hair, nails, lips and other human surfaces by rubbing, spraying or other similar methods for the purpose of cleaning, protecting, beautifying and modifying.

Plant extracts refer to plant products mainly composed of small biological molecules and macromolecules, which are formed by separating and purifying one or more effective ingredients in plant raw materials by physical, chemical and biological means. Compared with traditional cosmetics, cosmetics formulated with plant extracts as active ingredients have many advantages: they overcome the shortcomings of traditional cosmetics that rely on chemical synthetic products, making the product safer; natural plant extracts are more easily absorbed by the skin, and the effects of the resulting products are more significant; the functionality is more prominent, etc. The main functions of plant extracts in cosmetics are: moisturizing, anti-aging, freckle removal, sun protection, antiseptic, etc., and plant extracts are green and safe. Only by selecting the right plant extracts and adding the right amount of plant extracts to cosmetic products can their effects be maximized.

There is no public or reported research on extracting plant extracts from wormwood, sage, lavender and orange blossoms. At the same time, there are relatively few studies on further optimizing the extraction process to enhance the moisturizing and antioxidant effects of the extracts. Therefore, providing a technical solution to extract wormwood, sage, lavender and orange blossoms and further enhance the moisturizing and antioxidant effects is one of the technical problems that need to be solved urgently.

Summary of the invention

The present invention is proposed in view of the above technical problems. The present invention relates to the technical field of daily chemical products, and more specifically to an wormwood leaf extract and its application in cosmetics.

The technical solution of the present invention is specifically as follows.

On the one hand, the technical solution of the present invention includes an artemisia leaf extract, and the plant raw materials used in the preparation of the extract are: 1-4 parts of artemisia leaf, 0.5-3.5 parts of sage, 0.5-3 parts of lavender and 0.4-2.5 parts of orange blossom, calculated by mass.

Preferably, the preparation of the wormwood extract comprises the following steps:

Step 1: Mix wormwood leaves, sage, lavender and orange blossom according to the above mass parts, add water, and crush to obtain a crushed liquid;

Step 2: adding ethanol aqueous solution to the crushing liquid, stirring, and obtaining an extract;

Step 3: Concentrating the extract to obtain a concentrate, adding an extractant consisting of an oil phase and an aqueous phase to the concentrate, extracting, and pouring out the aqueous phase to obtain an extract;

Step 4: Filter the extract, concentrate the filtrate, and dry it to obtain the wormwood leaf extract.

Preferably, the preparation of the wormwood extract comprises the following steps:

Step 1: Mix wormwood leaves, sage, lavender and orange blossom according to the above mass parts, add water 1.5-3 times the mass of the plant raw materials, and crush them to obtain a crushed liquid;

Step 2: adding 45%-60% ethanol aqueous solution with a mass concentration of 4-10 times the mass of the crushing liquid to the crushing liquid, stirring for 20-60 hours to obtain an extract;

Step 3: Concentrate the extract to obtain a concentrate, add an extractant consisting of an oil phase and an aqueous phase to the concentrate, extract at 30° C.-50° C. for 4-10 hours, and pour out the aqueous phase to obtain an extract;

Step 4: filtering the extract, concentrating the filtrate, and drying to obtain the wormwood leaf extract;

The aqueous phase in the extractant is composed of water; the oil phase is composed of ethyl acetate and cyclohexane in a mass ratio of 1:3-5; the mass ratio of the aqueous phase to the oil phase is 1:2-4;

The mass ratio of the concentrate to the extractant in step 3 is 1:8-25.

Preferably, the mass ratio of the wormwood leaf to the sage is 1-2:1, 1.1-1.8:1 or 1.2-1.6:1.

Preferably, in step 1, the mass ratio of water to plant material is 1.8-3:1, 2-2.8:1 or 2.2-2.7:1.

Preferably, in step 2, the mass ratio of the ethanol aqueous solution to the crushing liquid is 5-10:1, 5.5-9.5:1 or 6-9:1.

Preferably, the mass concentration of the ethanol aqueous solution in step 2 is 46%-58%, 48%-55% or 50%-55%.

Preferably, the stirring in step 2 is carried out at a temperature of 35°C-60°C, 40°C-60°C, or 42°C-55°C.

Preferably, the stirring time in step 2 is 22-55 hours, 24-52 hours or 25-50 hours.

Preferably, the stirring speed in step 2 is 50-1000 rpm, 60-800 rpm or 70-500 rpm.

Preferably, the concentration in step 3 is reduced-pressure concentration, and the temperature of the reduced-pressure concentration is 55°C-65°C, 58°C-64°C or 60°C-63°C.

Preferably, in step 3, the extract is concentrated to 10%-25%, 12%-20% or 12%-18% of the mass of the extract.

Preferably, the mass ratio of the concentrate to the extractant in step 3 is 1:10-20, 1:12-18 or 1:12-16.

Preferably, the oil phase in step 3 is composed of ethyl acetate and cyclohexane in a mass ratio of 1:3-4.5, 1:3.5-4.5 or 1:3.8-4.2.

Preferably, the mass ratio of the water phase to the oil phase in step 3 is 1:2.5-4, 1:2.5-3.5 or 1:2.8-3.2.

Preferably, the extraction temperature in step 3 is 30°C-45°C, 32°C-45°C or 35°C-40°C.

Preferably, the extraction time in step 3 is 4.5-10 hours, 5-9 hours or 5.5-8 hours.

Preferably, in step 4, filtration is performed by membrane filtration, and the pore size of the membrane is less than 10 microns.

Preferably, the concentration in step 4 is carried out by vacuum distillation, and the temperature for the vacuum distillation is 35°C-45°C, 38°C-44°C or 39°C-43°C.

Preferably, in step 4, the concentration is to 10%-20%, 12%-18% or 12%-17% of the mass of the filtrate.

Preferably, the drying in step 4 is vacuum drying. The vacuum drying temperature is 45° C.-50° C., the pressure is 0.01 MPa-0.025 MPa, and the time is 3-20 hours.

On the other hand, the present invention also provides the use of the wormwood leaf extract in preparing cosmetics.

Preferably, the cosmetics include essence, lotion, facial mask, cream, ointment, foundation and the like.

Preferably, the mass dosage of the wormwood leaf extract in cosmetics is 0.01%-10%, 0.05%-8%, 0.1%-5%, 0.2%-2.5%, etc.

The beneficial effects of the present invention are as follows:

The invention adopts the method of adding water to wormwood, sage, lavender and orange blossom to crush them, then using ethanol water solution for extraction, and using an extractant obtained by combining an oil phase composed of ethyl acetate and cyclohexane with a water phase for extraction, and then performing post-treatment, so as to effectively extract oily active ingredients of wormwood, sage, lavender and orange blossom, such as components such as essential oils, and components such as organic matter, and give the extract excellent antioxidant and moisturizing effects.

The wormwood leaf extract obtained by the extraction method of the present invention has an excellent DPPH free radical scavenging effect, and the DPPH free radical scavenging efficiency reaches 87.52%.

The present invention uses a combination of wormwood and sage to further improve the effect of scavenging DPPH free radicals, and the two have a mutually promoting compound relationship in promoting the effect of scavenging DPPH free radicals. When the usage ratio of wormwood and sage reaches 1-2:1, the extract obtained by the preparation system has a better effect of scavenging DPPH free radicals.

The present invention uses an extractant obtained by combining an oil phase composed of ethyl acetate and cyclohexane with an aqueous phase for extraction, and the obtained extract has an excellent effect of removing DPPH free radicals; the oil phase of the present invention selects ethyl acetate and cyclohexane for extraction, which gives a better effect of removing free radicals. When the ratio of ethyl acetate to cyclohexane in the preparation system of the present invention is within the range of 1:3-5, the obtained extract has a better effect of removing DPPH free radicals.

The plant extract obtained by the method of the present invention has an excellent hydroxyl free radical scavenging effect, and the hydroxyl free radical scavenging efficiency reaches 82.34%.

The plant extract obtained by the extraction method of the present invention has an excellent effect of increasing the moisture content of the skin; after using the extract of the present invention for 2 hours, the moisture content of the skin can be increased by 45.72%; after using the extract of the present invention for 6 hours, the moisture content of the skin can be increased by 30.48%; after using the extract of the present invention for 10 hours, the moisture content of the skin can be increased by 18.54%.

The present invention uses an extractant obtained by combining an oil phase composed of ethyl acetate and cyclohexane with an aqueous phase for extraction, and the obtained extract has an excellent effect of increasing the moisture content of the skin; the extract obtained by combining ethyl acetate and cyclohexane in the oil phase of the present invention has a more significant effect of increasing the moisture content of the skin than the extractant obtained by other oil phases. When the ratio of ethyl acetate to cyclohexane in the preparation system of the present invention is within the range of 1:3-5, the obtained extract has a better effect of moisturizing the skin.

The product obtained by the invention has excellent effect and good application prospect.

Detailed ways

The technical solution of the present invention will be described below in conjunction with specific implementation methods, and the effects of the technical solution of the present invention will be made clearer and more specific.

1. Preparation of Extracts

Extract 1:

The specific preparation scheme is as follows: Step 1: Weigh plant raw materials according to mass: 3 parts of mugwort, 2 parts of sage, 1.2 parts of lavender and 0.8 parts of orange blossom; mix the plant raw materials, add 2.3 times the total mass of water of the plant raw materials, and crush them to obtain a crushed liquid;

Step 2: adding 52% ethanol aqueous solution with a mass concentration of 8 times the mass of the crushing liquid to the crushing liquid, stirring at a temperature of 45° C. and a rotation speed of 80 rpm for 30 hours to obtain an extract;

Step 3: The extract is concentrated under reduced pressure at a temperature of 62.5° C. to 15% of the mass of the extract to obtain a concentrate; an extractant consisting of an oil phase and an aqueous phase is added to the concentrate, and extraction is performed at 38° C. for 6.5 hours, and the aqueous phase is poured out to obtain an extract;

Step 4: Filter the extract through a filter membrane with a pore size of 1 micron, and concentrate the filtrate by vacuum distillation at 42°C to 15% of the filtrate mass; then vacuum dry at a temperature of 50°C and a pressure of 0.012MPa for 6.2 hours to obtain an extract;

Among them, the aqueous phase in the extractant is composed of water; the oil phase is composed of ethyl acetate and cyclohexane in a mass ratio of 1:4; the mass ratio of the aqueous phase to the oil phase is 1:3; the mass ratio of the concentrate to the extractant in step 3 is 1:12; the mass ratio of mugwort and sage in step 1 is 1.5:1.

Extract 2:

The specific preparation scheme is as follows: Step 1: Weigh plant raw materials according to mass: 2.5 parts of mugwort, 2.5 parts of sage, 1 part of lavender and 1 part of orange blossom; mix the plant raw materials, add 2.3 times the total mass of water of the plant raw materials, and crush them to obtain a crushed liquid;

Step 2: adding 55% ethanol aqueous solution with a mass concentration of 8 times that of the crushing liquid to the crushing liquid, stirring at a temperature of 43° C. and a rotation speed of 70 rpm for 25 hours to obtain an extract;

Step 3: The extract is concentrated under reduced pressure at 62° C. to 15% of the mass of the extract to obtain a concentrate; an extractant consisting of an oil phase and an aqueous phase is added to the concentrate, and extraction is performed at 40° C. for 6 hours, and the aqueous phase is poured out to obtain an extract;

Step 4: Filter the extract through a filter membrane with a pore size of 1 micron, and concentrate the filtrate by vacuum distillation at 40°C to 15% of the filtrate mass; then vacuum dry at a temperature of 52°C and a pressure of 0.014 MPa for 6 hours to obtain an extract;

Among them, the aqueous phase in the extractant is composed of water; the oil phase is composed of ethyl acetate and cyclohexane in a mass ratio of 1:3; the mass ratio of the aqueous phase to the oil phase is 1:3; the mass ratio of the concentrate to the extractant in step 3 is 1:12; the mass ratio of mugwort and sage in step 1 is 1:1.

Extract 3:

The specific preparation scheme is as follows: Step 1: Weigh plant raw materials according to mass: 5 parts of sage, 1.2 parts of lavender and 0.8 parts of orange blossom; mix the plant raw materials, add 2.3 times the total mass of water of the plant raw materials, and crush them to obtain a crushed liquid;

Step 2: adding 52% ethanol aqueous solution with a mass concentration of 8 times the mass of the crushing liquid to the crushing liquid, stirring at a temperature of 45° C. and a rotation speed of 80 rpm for 30 hours to obtain an extract;

Step 3: The extract is concentrated under reduced pressure at a temperature of 62.5° C. to 15% of the mass of the extract to obtain a concentrate; an extractant consisting of an oil phase and an aqueous phase is added to the concentrate, and extraction is performed at 38° C. for 6.5 hours, and the aqueous phase is poured out to obtain an extract;

Step 4: Filter the extract through a filter membrane with a pore size of 1 micron, and concentrate the filtrate by vacuum distillation at 42°C to 15% of the filtrate mass; then vacuum dry at a temperature of 50°C and a pressure of 0.012MPa for 6.2 hours to obtain an extract;

Among them, the aqueous phase in the extractant is composed of water; the oil phase is composed of ethyl acetate and cyclohexane in a mass ratio of 1:4; the mass ratio of the aqueous phase to the oil phase is 1:3; the mass ratio of the concentrate to the extractant in step 3 is 1:12.

Extract 4:

The specific preparation scheme is as follows: Step 1: Weigh plant raw materials according to mass: 5 parts of mugwort, 1.2 parts of lavender and 0.8 parts of orange blossom; mix the plant raw materials, add 2.3 times the total mass of water of the plant raw materials, and crush them to obtain a crushed liquid;

Step 2: adding 52% ethanol aqueous solution with a mass concentration of 8 times the mass of the crushing liquid to the crushing liquid, stirring at a temperature of 45° C. and a rotation speed of 80 rpm for 30 hours to obtain an extract;

Step 3: The extract is concentrated under reduced pressure at a temperature of 62.5° C. to 15% of the mass of the extract to obtain a concentrate; an extractant consisting of an oil phase and an aqueous phase is added to the concentrate, and extraction is performed at 38° C. for 6.5 hours, and the aqueous phase is poured out to obtain an extract;

Step 4: Filter the extract through a filter membrane with a pore size of 1 micron, and concentrate the filtrate by vacuum distillation at 42°C to 15% of the filtrate mass; then vacuum dry at a temperature of 50°C and a pressure of 0.012MPa for 6.2 hours to obtain an extract;

Among them, the aqueous phase in the extractant is composed of water; the oil phase is composed of ethyl acetate and cyclohexane in a mass ratio of 1:4; the mass ratio of the aqueous phase to the oil phase is 1:3; the mass ratio of the concentrate to the extractant in step 3 is 1:12.

Extract 5:

The specific preparation scheme is as follows: Step 1: Weigh plant raw materials according to mass: 1.6 parts of mugwort, 3.4 parts of sage, 1.2 parts of lavender and 0.8 parts of orange blossom; mix the plant raw materials, add 2.3 times the total mass of water of the plant raw materials, and crush them to obtain a crushed liquid;

Step 2: adding 52% ethanol aqueous solution with a mass concentration of 8 times the mass of the crushing liquid to the crushing liquid, stirring at a temperature of 45° C. and a rotation speed of 80 rpm for 30 hours to obtain an extract;

Step 3: The extract is concentrated under reduced pressure at a temperature of 62.5° C. to 15% of the mass of the extract to obtain a concentrate; an extractant consisting of an oil phase and an aqueous phase is added to the concentrate, and extraction is performed at 38° C. for 6.5 hours, and the aqueous phase is poured out to obtain an extract;

Step 4: Filter the extract through a filter membrane with a pore size of 1 micron, and concentrate the filtrate by vacuum distillation at 42°C to 15% of the filtrate mass; then vacuum dry at a temperature of 50°C and a pressure of 0.012MPa for 6.2 hours to obtain an extract;

Among them, the aqueous phase in the extractant is composed of water; the oil phase is composed of ethyl acetate and cyclohexane in a mass ratio of 1:4; the mass ratio of the aqueous phase to the oil phase is 1:3; the mass ratio of the concentrate to the extractant in step 3 is 1:12; the mass ratio of mugwort and sage in step 1 is 0.47:1.

Extract 6:

The specific preparation scheme is as follows: Step 1: Weigh plant raw materials according to mass: 4 parts of mugwort, 1 part of sage, 1.2 parts of lavender and 0.8 parts of orange blossom; mix the plant raw materials, add 2.3 times the total mass of water of the plant raw materials, and crush them to obtain a crushed liquid;

Step 2: adding 52% ethanol aqueous solution with a mass concentration of 8 times the mass of the crushing liquid to the crushing liquid, stirring at a temperature of 45° C. and a rotation speed of 80 rpm for 30 hours to obtain an extract;

Step 3: The extract is concentrated under reduced pressure at a temperature of 62.5° C. to 15% of the mass of the extract to obtain a concentrate; an extractant consisting of an oil phase and an aqueous phase is added to the concentrate, and extraction is performed at 38° C. for 6.5 hours, and the aqueous phase is poured out to obtain an extract;

Step 4: Filter the extract through a filter membrane with a pore size of 1 micron, and concentrate the filtrate by vacuum distillation at 42°C to 15% of the filtrate mass; then vacuum dry at a temperature of 50°C and a pressure of 0.012MPa for 6.2 hours to obtain an extract;

Among them, the aqueous phase in the extractant is composed of water; the oil phase is composed of ethyl acetate and cyclohexane in a mass ratio of 1:4; the mass ratio of the aqueous phase to the oil phase is 1:3; the mass ratio of the concentrate to the extractant in step 3 is 1:12; the mass ratio of mugwort and sage in step 1 is 4:1.

Extract 7:

The specific preparation scheme is as follows: Step 1: Weigh plant raw materials according to mass: 3 parts of mugwort, 2 parts of sage, 1.2 parts of lavender and 0.8 parts of orange blossom; mix the plant raw materials, add 2.3 times the total mass of water of the plant raw materials, and crush them to obtain a crushed liquid;

Step 2: adding 52% ethanol aqueous solution with a mass concentration of 8 times the mass of the crushing liquid to the crushing liquid, stirring at a temperature of 45° C. and a rotation speed of 80 rpm for 30 hours to obtain an extract;

Step 3: The extract is concentrated under reduced pressure at a temperature of 62.5° C. to 15% of the mass of the extract to obtain a concentrate; an extractant consisting of an oil phase and an aqueous phase is added to the concentrate, and extraction is performed at 38° C. for 6.5 hours, and the aqueous phase is poured out to obtain an extract;

Step 4: Filter the extract through a filter membrane with a pore size of 1 micron, and concentrate the filtrate by vacuum distillation at 42°C to 15% of the filtrate mass; then vacuum dry at a temperature of 50°C and a pressure of 0.012MPa for 6.2 hours to obtain an extract;

Among them, the aqueous phase in the extractant is composed of water; the oil phase is composed of ethyl acetate and cyclohexane in a mass ratio of 1:1; the mass ratio of the aqueous phase to the oil phase is 1:3; the mass ratio of the concentrate to the extractant in step 3 is 1:12; the mass ratio of mugwort and sage in step 1 is 1.5:1.

Extract 8:

The specific preparation scheme is as follows: Step 1: Weigh plant raw materials according to mass: 3 parts of mugwort, 2 parts of sage, 1.2 parts of lavender and 0.8 parts of orange blossom; mix the plant raw materials, add 2.3 times the total mass of water of the plant raw materials, and crush them to obtain a crushed liquid;

Step 2: adding 52% ethanol aqueous solution with a mass concentration of 8 times the mass of the crushing liquid to the crushing liquid, stirring at a temperature of 45° C. and a rotation speed of 80 rpm for 30 hours to obtain an extract;

Step 3: The extract is concentrated under reduced pressure at a temperature of 62.5° C. to 15% of the mass of the extract to obtain a concentrate; an extractant consisting of an oil phase and an aqueous phase is added to the concentrate, and extraction is performed at 38° C. for 6.5 hours, and the aqueous phase is poured out to obtain an extract;

Step 4: Filter the extract through a filter membrane with a pore size of 1 micron, and concentrate the filtrate by vacuum distillation at 42°C to 15% of the filtrate mass; then vacuum dry at a temperature of 50°C and a pressure of 0.012MPa for 6.2 hours to obtain an extract;

Among them, the aqueous phase in the extractant is composed of water; the oil phase is composed of ethyl acetate and cyclohexane in a mass ratio of 1:2; the mass ratio of the aqueous phase to the oil phase is 1:3; the mass ratio of the concentrate to the extractant in step 3 is 1:12; the mass ratio of mugwort and sage in step 1 is 1.5:1.

Extract 9:

The specific preparation scheme is as follows: Step 1: Weigh plant raw materials according to mass: 3 parts of mugwort, 2 parts of sage, 1.2 parts of lavender and 0.8 parts of orange blossom; mix the plant raw materials, add 2.3 times the total mass of water of the plant raw materials, and crush them to obtain a crushed liquid;

Step 2: adding 52% ethanol aqueous solution with a mass concentration of 8 times the mass of the crushing liquid to the crushing liquid, stirring at a temperature of 45° C. and a rotation speed of 80 rpm for 30 hours to obtain an extract;

Step 3: The extract is concentrated under reduced pressure at a temperature of 62.5° C. to 15% of the mass of the extract to obtain a concentrate; an extractant consisting of an oil phase and an aqueous phase is added to the concentrate, and extraction is performed at 38° C. for 6.5 hours, and the aqueous phase is poured out to obtain an extract;

Step 4: Filter the extract through a filter membrane with a pore size of 1 micron, and concentrate the filtrate by vacuum distillation at 42°C to 15% of the filtrate mass; then vacuum dry at a temperature of 50°C and a pressure of 0.012MPa for 6.2 hours to obtain an extract;

Among them, the aqueous phase in the extractant is composed of water; the oil phase is composed of ethyl acetate and cyclohexane in a mass ratio of 1:6.5; the mass ratio of the aqueous phase to the oil phase is 1:3; the mass ratio of the concentrate to the extractant in step 3 is 1:12; the mass ratio of mugwort and sage in step 1 is 1.5:1.

Extract 10:

The specific preparation scheme is as follows: Step 1: Weigh plant raw materials according to mass: 3 parts of mugwort, 2 parts of sage, 1.2 parts of lavender and 0.8 parts of orange blossom; mix the plant raw materials, add 2.3 times the total mass of water of the plant raw materials, and crush them to obtain a crushed liquid;

Step 2: adding 52% ethanol aqueous solution with a mass concentration of 8 times the mass of the crushing liquid to the crushing liquid, stirring at a temperature of 45° C. and a rotation speed of 80 rpm for 30 hours to obtain an extract;

Step 3: The extract is concentrated under reduced pressure at a temperature of 62.5° C. to 15% of the mass of the extract to obtain a concentrate; an extractant consisting of an oil phase and an aqueous phase is added to the concentrate, and extraction is performed at 38° C. for 6.5 hours, and the aqueous phase is poured out to obtain an extract;

Step 4: Filter the extract through a filter membrane with a pore size of 1 micron, and concentrate the filtrate by vacuum distillation at 42°C to 15% of the filtrate mass; then vacuum dry at a temperature of 50°C and a pressure of 0.012MPa for 6.2 hours to obtain an extract;

Among them, the aqueous phase in the extractant is composed of water; the oil phase is composed of ethyl acetate and cyclohexane in a mass ratio of 1:8; the mass ratio of the aqueous phase to the oil phase is 1:3; the mass ratio of the concentrate to the extractant in step 3 is 1:12; the mass ratio of mugwort and sage in step 1 is 1.5:1.

Extract 11:

The specific preparation scheme is as follows: Step 1: Weigh plant raw materials according to mass: 3 parts of mugwort, 2 parts of sage, 1.2 parts of lavender and 0.8 parts of orange blossom; mix the plant raw materials, add 2.3 times the total mass of water of the plant raw materials, and crush them to obtain a crushed liquid;

Step 2: adding 52% ethanol aqueous solution with a mass concentration of 8 times the mass of the crushing liquid to the crushing liquid, stirring at a temperature of 45° C. and a rotation speed of 80 rpm for 30 hours to obtain an extract;

Step 3: The extract is concentrated under reduced pressure at a temperature of 62.5° C. to 15% of the mass of the extract to obtain a concentrate; an extractant consisting of an oil phase and an aqueous phase is added to the concentrate, and extraction is performed at 38° C. for 6.5 hours, and the aqueous phase is poured out to obtain an extract;

Step 4: Filter the extract through a filter membrane with a pore size of 1 micron, and concentrate the filtrate by vacuum distillation at 42°C to 15% of the filtrate mass; then vacuum dry at a temperature of 50°C and a pressure of 0.012MPa for 6.2 hours to obtain an extract;

Among them, the aqueous phase in the extractant is composed of water; the oil phase is composed of petroleum ether; the mass ratio of the aqueous phase to the oil phase is 1:3; the mass ratio of the concentrate to the extractant in step 3 is 1:12; and the mass ratio of mugwort and sage in step 1 is 1.5:1.

2. Testing

1. DPPH free radical scavenging effect test

4 mg DPPH was diluted to 50 mL with anhydrous ethanol to obtain a DPPH solution; 0.5 mg extracts 1-11 were diluted to 50 mL with anhydrous ethanol to obtain the samples to be tested (according to the order of extracts 1-11, the samples to be tested were named sample A1-11 in sequence). The DPPH solution and the samples to be tested were sealed and placed in a light-proof environment at 37°C for 1 hour. The absorbance K value of each test solution was measured at 517 nm, and the DPPH free radical scavenging rate of the sample to be tested was calculated using the formula [1-(K1-K2)/K3]×100%, where K1 is the absorbance at 517 nm of a mixture of 2 mL of the sample to be tested and 2 mL of DPPH solution; K2 is the absorbance at 517 nm of a mixture of 2 mL of the sample to be tested and 2 mL of anhydrous ethanol; and K3 is the absorbance at 517 nm of a mixture of 2 ml of anhydrous ethanol and 2 mL of DPPH solution. The specific test results are shown in Table 1.

Table 1 DPPH free radical scavenging effect test

test DPPH free radical scavenging rate% Sample A1 87.52 Sample A2 86.21 Sample A3 78.36 Sample A4 82.45 Sample A5 80.27 Sample A6 84.13 Sample A7 77.34 Sample A8 82.54 Sample A9 83.92 Sample A10 80.48 Sample A11 72.37

According to the test results in Table 1, the present invention uses wormwood, sage, lavender and orange blossom to crush with water, then uses ethanol aqueous solution for extraction, and uses an extractant obtained by combining an oil phase composed of ethyl acetate and cyclohexane with an aqueous phase for extraction, and then performs post-treatment. The obtained plant extract has excellent DPPH free radical scavenging effect, and the DPPH free radical scavenging efficiency reaches 87.52%.

According to the test results of samples A1 and 3-4, the combination of wormwood and sage in the present invention can further improve the effect of scavenging DPPH free radicals, and the two have a mutually promoting compound relationship in promoting the effect of scavenging DPPH free radicals. When the usage ratio of wormwood and sage reaches 1-2:1, the extract obtained by the preparation system has a better effect of scavenging DPPH free radicals.

The present invention uses an extractant obtained by combining an oil phase composed of ethyl acetate and cyclohexane with an aqueous phase for extraction, and the obtained extract has an excellent effect of removing DPPH free radicals; through the test results of samples A1, A2 and A11, it can be obtained that the oil phase of the present invention selects ethyl acetate and cyclohexane for extraction to give a better effect of removing free radicals. And through the test results of A1 and A7-10, it can be obtained that when the ratio of ethyl acetate to cyclohexane in the preparation system of the present invention is within the range of 1:3-5, the obtained extract has a better effect of removing DPPH free radicals.

2. Hydroxyl free radical scavenging effect test

0.5 mg of extracts 1-11 were diluted to 50 mL with anhydrous ethanol to obtain the samples to be tested (according to the order of extracts 1-11, the samples to be tested were named sample B1-11).

Take 0.5mL of 0.75mmol/L phenanthroline ethanol solution in a container, add 2mL of 0.2mol/L phosphate buffer with a pH value of 7.4 and 1mL of the sample to be tested, mix well, add 0.5mL of 0.75mmol/L FeSO ₄ aqueous solution, mix well, add 0.5mL of 0.01% H ₂ O ₂ solution, mix well to obtain the test group solution.

Repeat the above test solution preparation steps, use an equal amount of deionized water to replace the sample to be tested to prepare a blank group solution.

Repeat the above test solution preparation steps, using an equal amount of deionized water to replace the H ₂ O ₂ solution to prepare a replacement group solution.

After the test group solution, blank group solution and replacement group solution prepared above were placed in a constant temperature water bath at 37°C for 1 hour, the absorbance values of the test solutions were measured at 536nm, which were recorded as A1, A2 and A3 values respectively. The hydroxyl radical scavenging rate of the sample to be tested was calculated using the formula [(A1-A3)/(A2-A3)]×100%. The specific results are shown in Table 2.

Table 2 Hydroxyl radical scavenging effect test

test Hydroxyl free radical scavenging rate% Sample B1 82.34 Sample B2 81.22 Sample B3 74.38 Sample B4 77.93 Sample B5 76.14 Sample B6 80.02 Sample B7 73.57 Sample B8 78.41 Sample B9 79.53 Sample B10 76.65 Sample B11 69.16

According to the test results in Table 2, the present invention uses wormwood, sage, lavender and orange blossom to crush with water, then uses ethanol aqueous solution for extraction, and uses an extractant obtained by combining an oil phase composed of ethyl acetate and cyclohexane with an aqueous phase for extraction, and then performs post-treatment. The obtained plant extract has excellent hydroxyl radical scavenging effect, and the hydroxyl radical scavenging efficiency reaches 82.34%.

3. Moisturizing effect test

The above extracts (extracts 1, 2, 7-11) were mixed with ethylene glycol and water in a mass ratio of 1:4:20 to obtain test samples (according to the order of extracts 1, 2, 7-11, the test samples were named as samples C1, 2, 7-11, respectively), and the moisturizing effect was tested.

The test method is as follows: Volunteers aged 30-55 were selected and randomly divided into 6 groups, with 3 male and 3 female volunteers in each group. The skin at the same position above the palm of the left arm of the volunteer was selected as the test area, with an area of 3×3 cm. Before applying the sample to be tested, the skin in the test area was cleaned with clean water and wiped dry with a woven cloth, and then the skin moisture content of the skin in the test area before applying the sample to be tested was tested using a CorneometerCM 825 test instrument and data was collected. Then each group of volunteers dripped the sample to be tested on the test area and applied it evenly, and the amount of the sample to be tested used in each test area of each volunteer was 0.2g. Then, the skin moisture content in the test area of the volunteer was tested using the same test method at 2 hours, 6 hours and 12 hours after the uniform application. Then, the increase ratio σ value of the skin moisture content at different times of using the sample to be tested compared to before using the test solution was calculated. Among them, the skin moisture content increase ratio _σ2 value after using the sample to be tested for 2 hours = (skin moisture content after using the sample to be tested for 2 hours - skin moisture content before using the sample to be tested) / skin moisture content before using the sample to be tested × 100%, the skin moisture content increase ratio _σ6 value after using the sample to be tested for 6 hours = (skin moisture content after using the sample to be tested for 6 hours - skin moisture content before using the sample to be tested) / skin moisture content before using the sample to be tested × 100%, and the skin moisture content increase ratio _σ10 value after using the sample to be tested for 10 hours = (skin moisture content after using the sample to be tested for 10 hours - skin moisture content before using the sample to be tested) / skin moisture content of the sample to be tested × 100%. The specific results are shown in Table 3.

Table 3 Moisturizing effect test

test _σ2 value/% σ ₆ value/% σ ₁₀ value/% Sample C1 45.72 30.48 18.54 Sample C2 44.23 29.57 17.86 Sample C7 38.62 26.12 15.35 Sample C8 42.37 28.43 17.03 Sample C9 41.28 27.76 16.54 Sample C10 39.51 26.62 15.76 Sample C11 35.45 24.15 13.92

According to the test results in Table 3, the present invention uses wormwood, sage, lavender and orange blossom to add water and crush, then use ethanol water solution for extraction, and use an extractant obtained by combining an oil phase composed of ethyl acetate and cyclohexane with an aqueous phase for extraction, and then perform post-treatment. The obtained plant extract has an excellent effect of increasing skin moisture content; after using the extract of the present invention for 2 hours, the proportion of skin moisture content can be increased by 45.72%; after using the extract of the present invention for 6 hours, the proportion of skin moisture content can be increased by 30.48%; after using the extract of the present invention for 10 hours, the proportion of skin moisture content can be increased by 18.54%.

The present invention uses an extractant obtained by combining an oil phase composed of ethyl acetate and cyclohexane with an aqueous phase for extraction, and the obtained extract has an excellent effect of increasing the moisture content of the skin; the test results of samples A1 and A2-11 show that the extract obtained by combining ethyl acetate and cyclohexane in the oil phase of the present invention has a more significant effect of increasing the moisture content of the skin than the extractant obtained by other oil phases. When the ratio of ethyl acetate to cyclohexane in the preparation system of the present invention is within the range of 1:3-5, the obtained extract has a better effect of moisturizing the skin.

The invention adopts the method of adding water to wormwood, sage, lavender and orange blossom to crush them, then using ethanol water solution for extraction, and using an extractant obtained by combining an oil phase composed of ethyl acetate and cyclohexane with a water phase for extraction, and then performing post-treatment, so as to effectively extract oily active ingredients of wormwood, sage, lavender and orange blossom, such as components such as essential oils, and components such as organic matter, and give the extract excellent antioxidant and moisturizing effects.

Those skilled in the art may make various changes and modifications to the present invention. If these changes and modifications fall within the scope of the claims of the present invention and their equivalents, the present invention is intended to include these changes and modifications.

Claims (10)

1. A mugwort leaf extract, characterized in that the plant raw materials used in the preparation of the extract are: 1-4 parts of mugwort leaf, 0.5-3.5 parts of sage, 0.5-3 parts of lavender and 0.4-2.5 parts of neroli by mass;

The preparation of the mugwort leaf extract comprises the following steps:

Step 1: mixing folium artemisiae argyi, sage, lavender and orange flower according to the mass parts, adding water with the mass 1.5-3 times of that of the plant raw materials, and crushing to obtain crushed liquid;

step 2: adding 45% -60% ethanol water solution with the mass concentration of 45-10 times of the mass of the crushing liquid into the crushing liquid, and stirring for 20-60 hours to obtain leaching liquid;

Step 3: concentrating the leaching solution to obtain a concentrate, adding an extractant consisting of an oil phase and a water phase into the concentrate, extracting for 4-10 hours at the temperature of 30-50 ℃, and pouring out the water phase to obtain an extract;

Step 4: filtering the extract, concentrating, and drying to obtain the folium Artemisiae Argyi extract;

Wherein the water phase in the extractant consists of water; the oil phase consists of ethyl acetate and cyclohexane according to the mass ratio of 1:3-5; the mass ratio of the water phase to the oil phase is 1:2-4;

The mass ratio of the concentrate to the extractant in the step 3 is 1:8-25.

2. The mugwort leaf extract of claim 1, wherein the mass ratio of mugwort leaf to sage is 1-2:1.

3. The mugwort leaf extract of claim 1, wherein the mass ratio of mugwort leaf to sage is 1.2-1.6:1.

4. The mugwort leaf extract of claim 1, wherein the oil phase is composed of ethyl acetate and cyclohexane in a mass ratio of 1:3.5-4.5.

5. The mugwort leaf extract of claim 1, wherein the mass ratio of the aqueous phase to the oil phase is 1:2.5-4.

6. The mugwort leaf extract of claim 1, wherein the mass ratio of concentrate to extractant in step 3 is 1:10-20.

7. The mugwort leaf extract of claim 1, wherein the stirring in step 2 is performed at a temperature of 40 ℃ to 60 ℃.

8. The mugwort leaf extract of claim 1, wherein the mass concentration of the aqueous ethanol solution in step 2 is 48% -55%.

9. The mugwort leaf extract of claim 1, wherein the extraction temperature is 32 ℃ to 45 ℃.

10. Use of the mugwort leaf extract of any of claims 1-9 in the manufacture of a cosmetic.