CN107279816A - A kind of preparation method of trepang compound soft capsule - Google Patents
A kind of preparation method of trepang compound soft capsule Download PDFInfo
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- CN107279816A CN107279816A CN201710369362.1A CN201710369362A CN107279816A CN 107279816 A CN107279816 A CN 107279816A CN 201710369362 A CN201710369362 A CN 201710369362A CN 107279816 A CN107279816 A CN 107279816A
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- soft capsule
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- sea cucumber
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/65—Addition of, or treatment with, microorganisms or enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicinal Preparation (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention belongs to biological technical field, specifically a kind of microwave radiation technology digests the preparation method of trepang compound soft capsule.Its preparation method is as follows：It is homogenized after fresh sea cucumber is removed the internal organs; digested under microwave radiation technology with compound protease; sequentially pass through inactivation, concentration, freeze-drying, grinding, sieving and obtain microwave radiation technology enzymolysis sea cucumber product dry powder, then compound chitosan oligosaccharide, taurine, beeswax and olive oil, seminal vesicle is filling to be formed.Microwave radiation technology enzymolysis trepang compound soft capsule has the fine effect such as anti-oxidant, anti-aging, fatigue-relieving and strengthen immunity in the present invention, with very strong nutrition and health care effect.
Description
Technical field
The present invention relates to biological technical field, specifically a kind of preparation method of trepang compound soft capsule.
Background technology
Sea cucumber belongs to Echinodermata Holothuroidea, is the traditional rare ocean treasure of China, nutritive value is high, and contains
Multiple beneficial in human body health bioactive substance, be a kind of nutriment of high protein, have " marine ginseng " it
Claim, and be listed in one of " eight delicacies ", with multiple efficacies such as anti-aging, anti-curing oncomas, also with edible well and medical treatment
The effect of aspect.The Ming Dynasty《Food book on Chinese herbal medicine》Point out, sea cucumber there are a variety of health maintenance effects；Main nourishing vigour, the beneficial the five internal organs six of taste
Internal organs and dispel it is deficient etc..The Qing Dynasty《A Supplement to the Compendium of Materia Medica》Sea cucumber is classified as help medicine.Successively there is the traditional Chinese medical science to propose in recent years with
Sea cucumber is alone or prescription treats tumour, aplastic anemia and diabetes etc. respectively, achieves good effect, while auxiliary
Assistant such as hypertension, hemorrhoid, neurasthenia, epilepsy, chronic nephritis, prolapse of the anus, gastric and duodenal ulcer, pulmonary tuberculosis, constipation, vein
It is widely used in the treatment of the common disease such as thrombus and chronic hepatitis B.
But the processing method of common sea cucumber destroys the nutritional ingredient and bioactivity of sea cucumber.
The content of the invention
The technical problems to be solved by the invention are the provision of a kind of preparation method of trepang compound soft capsule, solve existing
There is processing sea cucumber in technology, the problem of causing in sea cucumber bioactivity reduction and nutrition leak.
The present invention is achieved in that
A kind of preparation method of trepang compound soft capsule, this method includes：It is homogenized, utilizes after new fresh sea cucumber is removed the internal organs
Compound protease, is digested under the conditions of microwave radiation technology, enzymolysis product sequentially pass through inactivation, concentration, freeze-drying, grinding,
Microwave radiation technology enzymolysis sea cucumber product dry powder is made in sieving.
Further, selected microwave power is that 100W-300W carries out assistance enzymolysis, and wherein compound protein enzyme dosage is
3000U-4000U/g sea cucumber homogenates, are 15-20% in concentration of substrate, pH 6.0-6.5, hydrolysis temperature is enzyme at 55-65 DEG C
Solve 3-4h.
Further, the compound protease selection subtilopeptidase A complex.
Further, enzymolysis product is freezed dry in inactivating 5-10min at 95-100 DEG C after 40-50 DEG C of rotary evaporation concentration
It is dry, microwave solid assistance enzymolysis sea cucumber product is made.
Further, grinding, sieving process include：Sea cucumber product after inactivation is pulverized, using 150-200 mesh
Sub-sieve is sieved, and microwave radiation technology enzymolysis sea cucumber product dry powder is made.
Further, microwave radiation technology enzymolysis sea cucumber product dry powder is 7-9 according to mass ratio with chitosan oligosaccharide and taurine：1-3：
1-2 is mixed.
Further, mixed mixture is according to mass ratio 1:20-30 ratio is added in distilled water, stirring mixing
Uniformly it is spray-dried, homogenization microwave radiation technology enzymolysis trepang compound thing is made.
Further, the microwave radiation technology enzymolysis trepang compound thing of acquisition is added in miscella, and is heated under vacuum conditions
To 55-65 DEG C, 20-30min is stirred, is cooled to after 25-30 DEG C and refines filling form.
Further, the miscella is beeswax and olive oil, and mass ratio is 1：1-2.
Further, described microwave radiation technology enzymolysis trepang compound thing and miscella mass ratio are 1：6-9.
Compared with prior art, beneficial effect is the present invention：
Microwave assistant degradation be using microwave radiation technology react carry out a new technology, with it is easy to operate, pollution-free, can
The features such as control property is good, energy-efficient.Microwave be frequency about in 300MHz to 300GHz, wavelength is within 100m to 1mm scopes
Electromagnetic wave.Traditional mode of heating is heated from outside to inside by way of being the heat transfer thermal source, heat radiation, thermal convection current, is led to
Often result in heating slow, heated uneven etc..And microwave heating is then to vibrate to cause inside and outside same by molecule absorption microwave
Step heating, and microwave can penetrate container and material, it does not need conductive process, and heating effect is more preferable；In addition, microwave is heated
Than more uniform, it is to the overall of material while heating to be heated due to microwave, thus its homogeneous heating, without delayed；In addition, micro-
Alternative heating during Wave heating, because different material is different to microwave absorption degree, material molecular polarity is stronger, heating speed
Degree is faster；Also, microwave heating and energy saving is efficiently, only has those materials that can absorb microwave to be just heated in microwave field, and
Air will not generate heat with cell therefor, so the thermal efficiency is high.
Microwave radiation technology enzymolysis accelerates the principle of reaction process mainly to have following several respects.(1) thermal effect, it refers to microwave
The vibration frequency of molecule can be increased, so that rapid in reaction system produce amount of heat, priming reaction substrate accelerates reaction speed
Degree；(2) non-thermal effect is microwave by making the increase of polar molecule polarity, nonpolar molecule polarization, so that the knot of molecule
Structure changes；(3) microwave electromagnetic field can also be such that electronics is moved to specific direction by changing the electrical conductivity of reaction system；(4)
Microwave can also cause the vibration or rotation of molecule, cause the fracture of chemical bond, so as to improve the reaction rate of reaction system.Institute
Have that fast reaction rate, efficiency high, pollution-free, controllability be good, energy-efficient, enzymolysis product is lived with, microwave radiation technology enzymolysis sea cucumber
The advantages of property is strong.
Soft capsule is that one kind can be by oil or oily mater and the quantitative pressure injection of powdery functional materials suspension and encapsulating and glue
Seal capsule in film, with advantages below：(1) soft capsule has good isolation features, can be by the sensitive feature of light, oxygen
Material is isolated with air and light, so as to be prevented effectively from the oxidation of material, is that it is more stablized, is ensured bioactive substance effect
Performance；(2) flexible glue is quantitatively accurate, absorbs fast.After soft capsule is disintegrated in vivo, content is inhaled by body quickly in liquid form
Receive；(3) soft capsule surface is smooth, in good taste, easily swallows, and carries and the features such as instant.
Therefore, the present invention prepares sea cucumber enzymolysis product by the enzymolysis and extraction technique of innovation, obtains more more efficient lifes
Active substances, are then compounded by science, further strengthen its functional activity；Again by advanced soft capsule manner of packing,
More effectively preserve and discharge active component, realize the higher value application of Holothurian Resources, promote the development of human health cause.
Brief description of the drawings
Fig. 1 is the response surface experimental result diagram for the microwave radiation technology enzymolysis sea cucumber that the embodiment of the present invention 3 is provided；
Fig. 2 is that the embodiment of the present invention 3 provides the removing superoxide anion activity experiment knot that microwave radiation technology digests sea cucumber product
Fruit illustrates；
Fig. 3 is total reducing power activity experiment result diagram that the embodiment of the present invention 3 provides microwave radiation technology enzymolysis sea cucumber product；
Fig. 4 is the mouse anti-reflecting fatigue zoopery knot that the microwave radiation technology that the embodiment of the present invention 3 is provided digests trepang compound thing
Fruit illustrates.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Embodiment 1
It is homogenized after new fresh sea cucumber is removed the internal organs, takes 100g homogenates, add pH 6.0,50mM acetate buffers makes its bottom
Thing concentration reaches 15%, then adds in 300000U compound proteases, the present embodiment, compound protease selection hay bacillus egg
White combined enzyme agent, product specification：BR, 200U/mg, vigor：≥120units/mg.It is 100W, reaction temperature in microwave radiation technology condition
Spend to digest 3h at 55 DEG C.Sea cucumber hydrolysate is through 95 DEG C of inactivation 5min, after then rotation is concentrated by evaporation and is freeze-dried at 40 DEG C
Grind into powder, is sieved with 150 mesh sub-sieves, and microwave radiation technology enzymolysis sea cucumber product dry powder is made.Then, according to mass ratio
Sea cucumber product dry powder is digested for microwave radiation technology：Chitosan oligosaccharide：Taurine=7：1：1 is mixed, and is then added into distilled water
It is 1 to make its ratio:20, then stir and evenly mix and be spray-dried, prepare the microwave radiation technology enzymolysis trepang compound thing of homogenization；Most
Microwave-assisted enzyme is demultiplexed into square object by 1 afterwards:6 mass ratio adds propolis and olive oil mixture (1:1) in, and in vacuum state
Under be heated to 55 DEG C, stir 20min, be cooled to filling soft capsule after 25 DEG C.
Embodiment 2：
It is homogenized after new fresh sea cucumber is removed the internal organs, takes 100g homogenates, adding the 50mM acetate buffers of pH 6.5 makes its bottom
Thing concentration reaches 18%, then adds 350000U compound proteases, is 200W in microwave radiation technology condition, reaction temperature is 60 DEG C
Lower enzymolysis 3.5h.Sea cucumber hydrolysate is concentrated and pulverized after being freeze-dried through 98 DEG C of inactivation 8min, then 45 DEG C of rotary evaporations
End, is sieved with 180 mesh sub-sieves, and microwave radiation technology enzymolysis sea cucumber product dry powder is made.It is microwave-assisted enzyme according to mass ratio
Solve sea cucumber product dry powder：Chitosan oligosaccharide：Taurine=8：2：1 is mixed, and being then added into distilled water makes its ratio be
1:25, then stir and evenly mix and be spray-dried, the microwave radiation technology enzymolysis trepang compound thing of homogenization is made；It is finally that microwave is auxiliary
Enzymolysis trepang compound thing is helped by 1:7 ratio adds propolis and olive oil mixture (1:1.5) in, and heat under vacuum conditions
To 60 DEG C, 25min is stirred, filling soft capsule after 28 DEG C is cooled to.
Embodiment 3：
It is homogenized after new fresh sea cucumber is removed the internal organs, takes 100g homogenates, adding the 50mM acetate buffers of pH 6.5 makes its bottom
Thing concentration reaches 20%, then adds 400000U compound proteases, is 300W in microwave radiation technology condition, reaction temperature is 65 DEG C
Lower enzymolysis 4h.Sea cucumber hydrolysate is concentrated and pulverized after being freeze-dried through 100 DEG C of inactivation 10min, then 50 DEG C of rotary evaporations
End, is sieved with 200 mesh sub-sieves, and microwave radiation technology enzymolysis sea cucumber product dry powder is made.It is microwave-assisted enzyme according to mass ratio
Solve sea cucumber product dry powder：Chitosan oligosaccharide：Taurine=9：3：2 are mixed, and being then added into distilled water makes its ratio be 1:30,
Then stir and evenly mix and be spray-dried, the microwave radiation technology enzymolysis trepang compound thing of homogenization is made；Finally by microwave-assisted enzyme
Solve trepang compound thing and press 1:9 ratio adds propolis and olive oil mixture (1:2) in, and 65 are heated under vacuum conditions
DEG C, 30min is stirred, filling soft capsule after 30 DEG C is cooled to.
1 response surface is tested
Response surface optimization method, i.e. Response Surface Method (Response Surface Methodology, RSM), are a kind of real
The method for testing condition optimizing, by the basis of the response of each factor level, can find out prediction response optimal value and
Corresponding experiment condition.RSM is compared with orthogonal experiment, and its advantage is：, can be continuously to reality in experiment condition searching process
Each level tested is analyzed, and orthogonal experiment can only be analyzed experimental point isolated one by one.It is real by response surface
The efficiency of enzyme digestion reaction can intuitively be shown and search out optimum experimental condition by testing.
Using soluble protein content as response, on the basis of single factor experiment result, choose microwave power, enzyme dosage and
Enzymolysis time carries out response surface experiments design, and actual conditions is as follows：
Experimental result shows (participation Fig. 1), notable for auxiliary compound protease enzymolysis sea cucumber effect in microwave power.Its
In, it is microwave radiation technology power 300W, 60 DEG C, pH6.5, concentration of substrate through response surface optimization optimum enzymolysis condition：15%th, enzyme is used
Amount：4000U/g, enzymolysis time：4h.
Antioxidation activity in vitro experiment is carried out to the product of embodiment,
The removing ultra-oxygen anion free radical activity of 3 microwave radiation technologies enzymolysis sea cucumber product,
Remove superoxide anion activity experiment：Ultra-oxygen anion free radical (O2-) be free radical important in organism it
One, it is also the precursor of all active oxygen radicals, other active oxygen radicals can be converted into；During human metabolism
A large amount of free radicals can be produced, long term accumulation can cause the phenomenons such as numerous inflammation and aging to free radical in vivo.Remove super oxygen it is cloudy from
Sub- activity experiment is a kind of method of effective detection antioxidant activity in vitro, and the power of its Scavenging activity represents bioactive substance
Antioxidant activity in vitro it is strong and weak.
Microwave radiation technology is determined using PMS-NADH-NBT systems and digests sea cucumber product to the clear of ultra-oxygen anion free radical
Except effect.78 μM of NADH of 0.5mL, 0.5ml 10 μM of PMS of 50 μM of NBT, 0.5ml and various dose are sequentially added into test tube
Microwave radiation technology enzymolysis sea cucumber product, finally with 16mM, pH8.0Tris-HCl buffer solutions by system total volume most
3.0ml, makes its final concentration of：1、5、10、15、20、30、40、50mg/ml.After mixing, using AAS in 560nm ripples
The long lower absorbance for determining reaction solution.The 16mM of blank group same volume, pH8.0Tris-HCl buffer solution substitute test sample
And NADH, control group uses same volume 16mM, pH8.0Tris-HCl buffer solution to substitute test sample, and each concentration is repeated 3 times,
Experimental result is represented with clearance rate E:Experimental result shows (referring to Fig. 2) that microwave radiation technology is digested
Half clearance rate (IC of the removing ultra-oxygen anion free radical of sea cucumber product50) it is only 0.0675mg/ml, illustrate the microwave-assisted enzyme
Solving sea cucumber product has very strong removing ultra-oxygen anion free radical ability, shows very strong antioxidation activity in vitro.
4 microwave radiation technologies digest total reducing power of sea cucumber product
The power of the oxidation resistance of body defenses system is maintained close ties with health degree in the presence of this, and total reducing power is
One of important indicator of bioactive substance oxidation resistance is evaluated, the size of total reducing power then directly reflects that its is anti-oxidant
The power of ability.
The microwave radiation technology enzymolysis sea cucumber product of 1.0mL various concentrations is sequentially added into test tube makes its final concentration be respectively：
1st, 5 then, 10,15,20,30,40,50mg/mL, 1mL 1% potassium ferricyanide, 50 DEG C of water-bath 20min add 2mL's 10%
After trichloroacetic acid terminating reaction, 5min, 1.25mL0.1% ferric trichloride is added, is well mixed, 30min is stood, in 700nm
Colorimetric.Blank group replaces sample with 0.2mol/L pH 6.6PBS.Experimental result shows (referring to Fig. 3) that microwave radiation technology is digested
Sea cucumber product, which has, has good linear relationship between good reducing power, and its total reducing power and concentration of substrate,
It is an extraordinary antioxidant.
Antifatigue experiment in the Mice Body of 5 microwave radiation technologies enzymolysis trepang compound thing：
Mouse anti-reflecting fatigue zoopery：Exercise induced fatigue refers to that body ability to work is temporarily reduced caused by motion itself,
The physiological phenomenon that can recover through appropriate time rest and adjustment.Fatigue both indicated the original ability to work of body it is temporary transient under
Drop, is probably the tendency that body develops into sick and wounded state again.Antifatigue is exactly the generation of delay fatigue, and what is accelerated fatigue disappears
Remove；Exercise tolerance experiment such as mice burden swimming experiment is the best macro manifestations for weighing anti-fatigue ability, passes through mouse
It is antifatigue experiment then can test organisms active material internal anti-fatigue ability.
Experiment is divided into 5 groups, every group of Kunming kind SPF mouse of 10 male and female half and half：(1) blank control group；(2) positive controls
(American Ginseng 0.3mg/g.d)；(3) low dose group (0.05mg/g.d)；(4) middle dose group (0.15mg/g.d)；(5) high dose group
(0.5mg/g.d).Blank control group gavage distilled water, administration frequency is 1 times/day, and administration time is 4 weeks.It is 5 groups small by observing
Mouse is after last gavage 30min, the body weight sheet lead of mouse tail root load 5%, is placed in swimming trunk that (25 ± 1 DEG C of water temperature, the depth of water is not
Less than 30cm) swimming, 10s does not refloat after being sunk under water to mouse exhausts death standard as power, record from swim till death
The time is died, mouse swimming time is used as.Power exhausts swimming test and is widely used in animal model evaluating novel active thing
Matter fatigue resistance, mouse swimming time is longer, illustrates that the effect of its fatigue-relieving is more obvious.Experimental result show (referring to
Fig. 4), microwave radiation technology enzymolysis trepang compound thing has very strong internal anti-fatigue ability, and its anti-fatigue ability compares positive control
American Ginseng is stronger, is very effective anti-fatigue active material.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
Any modifications, equivalent substitutions and improvements made within refreshing and principle etc., should be included in the scope of the protection.
Claims (10)
1. a kind of preparation method of trepang compound soft capsule, it is characterised in that this method includes：
It is homogenized, using compound protease, is digested under the conditions of microwave radiation technology, enzymolysis product after new fresh sea cucumber is removed the internal organs
Sequentially pass through inactivation, concentration, freeze-drying, grinding, the obtained microwave radiation technology enzymolysis sea cucumber product dry powder of sieving.
2. the preparation method of the trepang compound soft capsule according to claim 1, it is characterised in that selected microwave power is
100W-300W carries out assistance enzymolysis, and wherein compound protein enzyme dosage is 3000U-4000U/g sea cucumber homogenates, in concentration of substrate
For 15-20%, pH 6.0-6.5, hydrolysis temperature is to digest 3-4h at 55-65 DEG C.
3. the preparation method of the trepang compound soft capsule according to claim 2, it is characterised in that the compound protease selection is withered
Careless Bacillus protease complex.
4. the preparation method of the trepang compound soft capsule according to claim 1, it is characterised in that enzymolysis product is at 95-100 DEG C
5-10min is inactivated, is freeze-dried after 40-50 DEG C of rotary evaporation concentration, microwave solid assistance enzymolysis sea cucumber product is made.
5. the preparation method of the trepang compound soft capsule according to claim 1, it is characterised in that grinding, sieving process include：Through
Sea cucumber product after inactivation is pulverized, and is sieved using 150-200 mesh sub-sieves, and microwave radiation technology enzymolysis sea cucumber production is made
Thing dry powder.
6. the preparation method of the trepang compound soft capsule according to claim 1, it is characterised in that microwave radiation technology digests sea cucumber product
Dry powder is 7-9 according to mass ratio with chitosan oligosaccharide and taurine：1-3：1-2 is mixed.
7. the preparation method of the trepang compound soft capsule according to claim 6, it is characterised in that mixed mixture is according to matter
Amount compares 1:20-30 ratio is added in distilled water, is uniformly mixed and is spray-dried, and homogenization microwave-assisted enzyme is made
Solve trepang compound thing.
8. the preparation method of the trepang compound soft capsule according to claim 7, it is characterised in that the microwave radiation technology enzymolysis sea of acquisition
Join compound thing to add in miscella, and be heated to 55-65 DEG C under vacuum conditions, stir 20-30min, be cooled to after 25-30 DEG C
Refine filling form.
9. the preparation method of the trepang compound soft capsule according to claim 8, it is characterised in that the miscella is beeswax and olive
Olive oil, mass ratio is 1：1-2.
10. the preparation method of the trepang compound soft capsule according to claim 8, it is characterised in that described microwave radiation technology enzymolysis
Trepang compound thing is 1 with miscella mass ratio：6-9.
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| GB2353280A (en) * | 1999-05-03 | 2001-02-21 | Procter & Gamble | Methods for activating enzymes |
| CN1781494A (en) * | 2004-12-03 | 2006-06-07 | 王伟成 | Preparation method of sea cucumber active ingredient soft capsule |
| CN101341978A (en) * | 2008-08-13 | 2009-01-14 | 赵兴坤 | Holothurian peptide functional food and preparation method thereof |
| CN101897458A (en) * | 2010-07-06 | 2010-12-01 | 丹东阿里郎生物工程有限公司 | Seacucumber-blueberry instant particles and processing method thereof |
| CN102940280A (en) * | 2012-10-26 | 2013-02-27 | 青岛佳日隆海洋食品有限公司 | Sea cucumber capsule and preparation process thereof |
| KR101401904B1 (en) * | 2012-11-27 | 2014-05-30 | 김지현 | Low temperature drying method of abalone and sea slug using micro wave generated from magnetron which is installed in refrigeration facilities |
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2017
- 2017-05-23 CN CN201710369362.1A patent/CN107279816B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2353280A (en) * | 1999-05-03 | 2001-02-21 | Procter & Gamble | Methods for activating enzymes |
| CN1781494A (en) * | 2004-12-03 | 2006-06-07 | 王伟成 | Preparation method of sea cucumber active ingredient soft capsule |
| CN101341978A (en) * | 2008-08-13 | 2009-01-14 | 赵兴坤 | Holothurian peptide functional food and preparation method thereof |
| CN101897458A (en) * | 2010-07-06 | 2010-12-01 | 丹东阿里郎生物工程有限公司 | Seacucumber-blueberry instant particles and processing method thereof |
| CN102940280A (en) * | 2012-10-26 | 2013-02-27 | 青岛佳日隆海洋食品有限公司 | Sea cucumber capsule and preparation process thereof |
| KR101401904B1 (en) * | 2012-11-27 | 2014-05-30 | 김지현 | Low temperature drying method of abalone and sea slug using micro wave generated from magnetron which is installed in refrigeration facilities |
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