CN102613567B - Soybean peptide nutrient solution and preparation method and application thereof - Google Patents
Soybean peptide nutrient solution and preparation method and application thereof Download PDFInfo
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
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- A23L33/17—Amino acids, peptides or proteins
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Abstract
The invention discloses a soybean peptide nutrient solution, which is prepared by extracting pale yellow clear liquid of soybeans. An extraction method comprises the following steps of 1) electromagnetic cracking, 2) press extraction, 3) filtering concentration, 4) drying extraction and 5) merging extraction liquid. The soybean peptide nutrient solution is rich in small molecule polypeptide and soybean isoflavone and has good prevention and treatment effects on a variety of diseases including chronic hepatitis b, acne, gastric ulcer or superficial gastritis and the like. A preparation method of the soybean peptide nutrient solution is further provided.
Description
Technical field
The present invention relates to a kind of health food, be specifically related to a kind of soya-bean polypeptides nutrient solution, and its preparation method and application.
Background technology
Protein is the substance basis of life, is the human body indispensable nutriment that grows, and protein is made of several amino acids, wherein is called oligopeptides by 3～10 amino acid with the compound that peptide bond links to each other; Be called polypeptide by 10～50 amino acid with the compound that peptide bond links to each other; 50 are called protein with upper amino acid with the compound that peptide bond links to each other.
After always people think that protein is taken in, must it be hydrolyzed into amino acid whose form through the various enzymes of alimentary canal and just can be absorbed.In recent years, a large amount of result of study of scientist shows: protein not is that complete hydrolysis becomes amino acid after taking in, but major part just is absorbed when the form of peptide.Find that simultaneously the absorbed speed of polypeptide is faster than free amino acid, absorptivity is higher, particularly little molecule oligopeptides.
Protein is as nutriment, mainly form and the part free amino acid with peptide could absorb in human body, bring into play various physiological functions, thus, polypeptide is very close with healthy relation, he reconciles and is arranging life from being born to the overall process of death, replenishes nutrient protein take the form of polypeptide as best to human body.Different crowds is replenished the allogenic polypeptide goods in right amount, immunologic function is prevented and cured diseases, strengthened to human body, it is very important improving body health quality.
The protein of necessary for human mainly is to absorb in driven, the vegetable protein food.Recent studies shows that animal protein contains a large amount of fat, cholesterol, the much more edible generation that can cause many " rich man's diseases " such as hypertension, high fat of blood, coronary heart disease.Vegetable protein substitutes animal protein and is paid attention to by people.
Vegetable protein is take cereal as main, and soy protein content is up to more than 40% in the cereal, and the amino acid kind that forms soybean protein is more complete.Wherein, contain the amino acid that 9 kinds of human bodies can not synthesize voluntarily, be called " necessary amino acid ", amino acid balance is good.Soya-bean polypeptides, united state food and agricultural organization is recommended as " the ideal source of phytoprotein ".
Research to soy bean protein hydrolysate starts from the sixties in 19th century, is at that time to use acid, alkali under fixed temperature catalytic pyrolysis.But many defectives such as this chemical method causes the nutritional labeling loss excessive, and side reaction is many, and organoleptic properties is poor.To the eighties in 19th century, because the speed of enzymology technology hails exhibition, the U.S., Japan have obtained larger progress with enzymatic process, to the eighties in 20th century, Japanology more comprehensive, carrying is not physicochemical characteristic, different physiological roles and application system comparatively all to soya-bean polypeptides, and developed several functions food, resistance should living infant food, the nutritious supplementary pharmaceutical such as sports food.
At present, it all is enzyme hydrolysis process that countries in the world produce soya-bean polypeptides, this method, complex technical process, and the bitter taste of hydrolysate still can not solve at all.Exist many drawbacks such as degree of hydrolysis is low, weak flavor (bitter taste), complex technical process, production cost height, limited the demand in application and market, anxious to be resolved is: seek new method, new technology.
China is the native place of soybean, before more than 2000 years, and founder of the Han Dynasty's grandson. Huainan king Liu An, in " Mt. Bagong " in Jiangsu makes pills of immortality process, invented with soybean and made " bean curd ", spread all over all over the world so far, China only is in the preliminary working stage of bean product.Until the later stage eighties in 20th century is just carried out primary study to soya-bean polypeptides, and list exploitation and the application of " soya-bean polypeptides " in the enforcement period of the ninth five-year plan national key scientific and technological project.China National Academy of Food ﹠ Fermentation Industries is the earliest to the research institution of soya-bean polypeptides, and has born " 95 " brainstorm project.Now finish soybean or dregs of beans and extracted the task of separating soya-bean polypeptides.Used enzymolysis process and soya-bean polypeptides product have all reached world level, maintain the leading position at home, but not yet scale of input production, product is not still gone public.
According to the latest survey result, the method for producing soya-bean polypeptides both at home and abroad all is enzyme hydrolysis process, all has following problem:
(1) being used for the enzyme kind of hydrolysis, more single (only have trypsase, alkali protease, neutral proteinase, papain several, degree of hydrolysis is low, hydrolysate weak flavor (bitter taste is arranged).
(2) enzymolysis mechanism, degree of hydrolysis and zymolyte characteristic relation are indefinite, cause hydrolysis process control to lack theoretical direction, and control is difficult.
(3) bitter taste of hydrolysis still can not solve at all, and application is restricted.
(4) the enzymolysis process process is complicated: soybean, and---------hydrolysate separation---debitterize flavor---desalination etc. cause production cost high to protein isolate in enzyme hydrolysis.
Above problem: degree of hydrolysis is low, weak flavor, poor quality control, production cost are high, make the soya-bean polypeptides product be difficult to appear on the market.
Summary of the invention
The object of the invention is to: a kind of soya-bean polypeptides nutrient solution is provided, various diseases is had significant prevention effect.
Above-mentioned purpose of the present invention is achieved through the following technical solutions:
A kind of soya-bean polypeptides nutrient solution is provided, and it is that described extracting method may further comprise the steps by the light yellow clarifying liquid body of physical method extraction from soybean:
1) electromagnetic dissociation: soybean is entered in the airtight high-frequency and high-voltage alternating electromagnetic field continuously, add thermal material to 100～110 ℃, collect the steam and the condensation that produce, obtain liquid I and solid material I;
2) the solid material I that squeezing lixiviate: with step 1) obtains oil meal after the routine oil expression is processed separates, and the dregs of beans that obtains adds the water soaking of 3 times of weight, and stirs after 30 minutes under ultrasonic vibration and carry out Separation of Solid and Liquid, obtains solid material II and liquid II;
3) the liquid II standing demix that filtering and concentrating: with step 2) obtains obtains the supernatant on upper strata and the dirty solution of lower floor, and supernatant by the molecular sieve filtration of molecular weight below 2000, is leached liquid III; It is concentrated to heat after the liquid by molecular sieve and dirty solution do not mix, and collects the steam and the condensation that produce, until percent by weight of dry matter content 〉=30% in the mixed liquor, obtains congee shape solid material III and liquid IV after concentrated;
4) the solid material II and the step 3 that dry extracting: with step 2) obtain) the solid material III that obtains dry processing the respectively, until wherein water content drops to below 10%, collect the steam and the condensation that produce therebetween and be mixed to get liquid V;
5) merge extract: with step 1) the liquid I, the step 3 that obtain) the liquid III and liquid IV and the step 4 that obtain) the liquid V that obtains mixes.
Step 1) can finish by existing various electromagnetic dissociation equipment, preferably adopting the patent No. is that the electromagnetic dissociation device of putting down in writing in the utility model patent specification of ZL200420059838 is finished.This device comprises totally enclosed silo, material import and export, electromagnetic generator, spiral release cage device and condenser; Described material inlet is bucket shape, is arranged at the top of silo, and material outlet is tubular, is arranged at the below of silo, spiral is installed in it releases the cage device, and this spiral is released the cage device and driven by gearbox and buncher; Described electromagnetic generator comprises a high-frequency signal generating source, be fixed in the coniform electromagnetism anode of central authorities in the silo and form with three parts of silo as the electromagnetism negative electrode; Described condenser is connected with silo inside, and an axial flow blower is set at the condensator outlet place; The output frequency of described high-frequency signal generating source is 200KHZ to 2000MHZ, and power output is in 100W to 100KW scope.Enter the cracking storehouse of silo formation when material after, effect by the high-frequency alternating electromagnetic field, material is warming up to 100～110 ℃, molecule in the material is under the alternating electromagnetic field impact, constantly reverse, stretch, make its molecular breakdown, moisture in the material is lowered the temperature by condenser after being heated to form steam, in the water that collect at the condenser place, contains multiple micromolecule polypeptide composition.
Step 2) the preferred screw oil expeller that adopts of described oil expression processing is finished; Described Separation of Solid and Liquid preferably adopts disk centrifuge to finish.
Step 5) mixed liquor that obtains is preferably further processed through ultraviolet disinfection.
The present invention also provides the preparation method of described soya-bean polypeptides nutrient solution, may further comprise the steps:
1) electromagnetic dissociation: soybean is entered in the airtight high-frequency and high-voltage alternating electromagnetic field continuously, add thermal material to 100～110 ℃, collect the steam and the condensation that produce, obtain liquid I and solid material I;
2) the solid material I that squeezing lixiviate: with step 1) obtains oil meal after the routine oil expression is processed separates, and the dregs of beans that obtains adds the water soaking of 3 times of weight, and stirs after 30 minutes under ultrasonic vibration and carry out Separation of Solid and Liquid, obtains solid material II and liquid II;
3) the liquid II standing demix that filtering and concentrating: with step 2) obtains obtains the supernatant on upper strata and the dirty solution of lower floor, and supernatant by the molecular sieve filtration of molecular weight below 2000, is leached liquid III; It is concentrated to heat after the liquid by molecular sieve and dirty solution do not mix, and collects the steam and the condensation that produce, until percent by weight of dry matter content 〉=30% in the mixed liquor, obtains congee shape solid material III and liquid IV after concentrated;
4) the solid material II and the step 3 that dry extracting: with step 2) obtain) the solid material III that obtains dry processing the respectively, until wherein water content drops to below 10%, collect the steam and the condensation that produce therebetween and be mixed to get liquid V;
5) merge extract: with step 1) the liquid I, the step 3 that obtain) the liquid III and liquid IV and the step 4 that obtain) the liquid V that obtains mixes.
Step 1) preferably adopting the patent No. is that the electromagnetic dissociation device of putting down in writing in the utility model patent specification of ZL200420059838 is finished.
Step 2) the preferred screw oil expeller that adopts of described oil expression processing is finished; Described Separation of Solid and Liquid preferably adopts disk centrifuge to finish.
Step 5) mixed liquor that obtains is preferably further processed through ultraviolet disinfection.
The present invention also provides the application of described soya-bean polypeptides nutrient solution in the health food of preparation control chronic hepatitis B, acne, gastric ulcer or superficial gastritis.
Soya-bean polypeptides nutrient solution of the present invention has following beneficial effect:
1. soya-bean polypeptides nutrient solution active component of the present invention is abundant, according to detection, the soya-bean polypeptides nutrient solution of the embodiment of the invention 1 preparation contains 23 peptide species (molecular weight is below 1000Dalton, and chromatography the results are shown in Figure 1) and 18 kinds of free amino acid (9 kinds of necessary amino acid are wherein arranged) and 6 kinds of vitamins; Still contain 4 kinds of activity of fighting against senium materials: reduced form Agifutol (GSH), glutathione-S transferase (GSH-S), catalase (CAT), superoxide dismutase (SOD), the nutrient composition content mean value of concrete sample sees Table 1-3:
Table 1. amino acid contamination
Table 2. vitamin contamination
Table 3. antioxidant content and content
| Antioxidase | Content |
| Reduced glutathione (GSH) | 110μg/ml |
| TAC: | 5.57U/ml |
| Glutathione-S transferase (GSH-S) | 192.00U/ml |
| Catalase (CAT) | 7.390×10-3U/ml |
| Superoxide dismutase (SOD) | 0.27μg/ml |
2. soya-bean polypeptides nutrient solution features good taste of the present invention is without bitter taste, odorless, fragrance slightly.
3. production method of the present invention is pure physical method, therefore in the whole preparation process without any additive, guaranteed pure property and the security of product.
4. production cost of the present invention is low, and technique is simple, easy to operate, the cracking degree high.
Nutrient solution of the present invention is because preparation method's cost is low, thereby the market price is relatively low, is easily accepted by the consumer, and application is wide, and the adaptation population is many, and DEVELOPMENT PROSPECT is good.
Description of drawings
Fig. 1 is the photo that comprises polypeptide chromatography figure in the soya-bean polypeptides nutrient solution of embodiment 1 preparation and data thereof.
The specific embodiment
Embodiment 1. Preparation Examples
A kind of soya-bean polypeptides nutrient solution, extracting method may further comprise the steps:
1) soybean is entered continuously in the electromagnetic dissociation device described in the ZL200420059838 utility model patent specification specific embodiment, add thermal material to 100～110 ℃, collect the steam and the condensation that produce, obtain liquid I and solid material I;
2) with step 1) the solid material I that obtains adopts screw oil expeller oil meal after the routine oil expression is processed to separate, the dregs of beans that obtains adds the water soaking of 3 times of weight, and under ultrasonic vibration, stir and adopt disk centrifuge to carry out Separation of Solid and Liquid after 30 minutes, obtain solid material II and liquid II;
3) with step 2) the liquid II standing demix that obtains, obtain the supernatant on upper strata and the dirty solution of lower floor, supernatant by the molecular sieve filtration of molecular weight below 2000, is leached liquid III; It is concentrated to heat after the liquid by molecular sieve and dirty solution do not mix, and collects the steam and the condensation that produce, until percent by weight of dry matter content 〉=30% in the mixed liquor, obtains congee shape solid material III and liquid IV after concentrated;
4) with step 2) the solid material II and the step 3 that obtain) the solid material III that obtains dry processing the respectively, until wherein water content drops to below 10%, collect the steam and the condensation that produce therebetween and be mixed to get liquid V;
5) with step 1) the liquid I, the step 3 that obtain) the liquid III and liquid IV and the step 4 that obtain) the liquid V that obtains mixes, and obtains soya-bean polypeptides nutrient solution of the present invention.
The effect clinical report
Soya-bean polypeptides nutrient solution of the present invention has significant prevention effect to various diseases, and concrete effect is seen following experiment:
1 soya-bean polypeptides nutrient solution of the present invention is to the clinical observation on the therapeutic effect of chronic hepatitis B
[observational technique]:
Diagnostic method: " new Chinese medicine guideline of clinical investigations (1993) " diagnostic criteria of developing and publishing according to Ministry of Health of the People's Republic of China.
Group technology: be divided at random 2 groups, every group of 30 people.Control group administration amino acid (animal blood) capsule, each 3, every day 3 times; The soya-bean polypeptides nutrient solution of the treatment group administration embodiment of the invention 1 preparation, each oral 20ml, every day 3 times.
The course for the treatment of: 1-2 month is a course for the treatment of;
Methods for the treatment of: symptom of each comprehensive review, sign, the full item of liver function, immunoglobulin (Ig), blood, routine urinalysis, various viral antigen health check-up survey, liver and gall spleen B ultrasonic before and after the treatment;
Efficacy determination: " new Chinese medicine guideline of clinical investigations (1993) " curative effect determinate standard of developing and publishing according to Ministry of Health of the People's Republic of China.
The basic healing: subjective symptoms disappears, and hepatosplenomegaly is stablized constant or dwindled, and without tenderness and kowtow pain, liver function test is normal, and virus detects and turns out cloudy, and above indices was stablized more than 1 year.
Produce effects: subjective symptoms disappears, and hepatosplenomegaly is stablized constant, and without obvious tenderness with kowtow pain, the every obvious recovery of liver function or normal, and more than lasting half a year.
Effectively: cardinal symptom disappears or basic the disappearance, and hepatosplenomegaly is stable and without obvious tenderness and kowtow pain, the every inspection of liver function is normally understood initial value and descended more than 50%, and continues three months.
Invalid: after finishing the course for the treatment of, the unchanged or deterioration of indices.
[observed result]:
The soya-bean polypeptides nutrient solution 1-2 that treatment group 30 routine clothes for patients are used the embodiment of the invention 1 is after each course for the treatment of, and clinical cure 5 examples account for 16.6%, and produce effects 8 examples account for 26.7%, and effective 14 examples account for 46.7%, and invalid 3 examples account for 10%.Total effective rate is 90%, sees table 4 for details.
Table 4
As can be seen from Table 1, the soya-bean polypeptides nutrient solution of the embodiment of the invention 1 has good result for the treatment of to chronic hepatitis B.
Table 5
As can be seen from Table 5, treatment group surface antigen negative conversion rate can reach 30.4%, e antigen negative conversion rate can reach 27.8%, significantly is better than control group.
2 soya-bean polypeptides nutrient solutions of the present invention are observed the result for the treatment of of acne
The 29 routine patients of dept. of dermatology have been carried out nine months clinical observation, added outer wiping use through taking orally, found recovery from illness 16 examples, accounted for 55.17%, 10 examples that take a turn for the better account for 34.48%, and invalid 3 examples account for 10.39%.
Research thinks that several amino acids, the vitamins composition in the soya-bean polypeptides nutrient solution of the present invention has the effect of regulating nutrition to skin metabolism, and soybean includes flavonoids simultaneously, has the endocrine function of adjusting.3 soya-bean polypeptides nutrient solutions of the present invention are to the clinical observation on the therapeutic effect of gastric ulcer
[diagnostic criteria of gastric ulcer]: by the clinical guidance principle of the nineteen ninety-five new Chinese medicine treatment gastric ulcer developed and published of the Ministry of Public Health.
Western medicine diagnose standard: with reference to the standard of the whole nation, nineteen eighty-two Chongqing gastritis diagnosis and treatment forum formulation;
Differential diagnosis in tcm: with reference to the diagnosis tentative standard of disease of digestive system Professional Committee of CAIM in 1989.
Curative effect determinate standard: according to the clinical guidance principle regulation of the nineteen ninety-five new Chinese medicine treatment gastric ulcer developed and published of the Ministry of Public Health:
1. clinical cure: the clinical symptoms sign disappears, and gastroscope check mucous membrane chronic inflammation takes a turn for the better, and intestinesization are given birth to and dysplasia recovers normal or disappearance;
2. produce effects: clinical cardinal symptom, sign disappear, and gastrocopy mucous membrane chronic inflammation takes a turn for the better, and intestinesization are given birth to and dysplasia recovers normal or alleviate 2 spends;
3. effective: the cardinal symptom sign obviously alleviates, and gastroscope check mucosa infection scope is dwindled more than 1/2, and inflammation alleviates to some extent, and ulcer surface reduces; Intestinesization are given birth to and dysplasia alleviates;
4. invalid: as not reach effective standard or anti-the deterioration.
[methods for the treatment of]:
The soya-bean polypeptides nutrient solution of the treatment group administration embodiment of the invention 1, every day 2 times, each 20ml, one month is a course for the treatment of, individual month of the heavy continuously 2-3 of the state of an illness, viewing duration all strengthening spleen and nourishing stomach medicines of stopping using.Respectively check OGD and every physico-chemical examination before and after the treatment, the result is as follows:
The total effective rate of table 6 soya-bean polypeptides nutrient solution treatment of the present invention gastric ulcer
| Grouping | Total routine number | Clinical cure | Produce effects | Effectively | Invalid | Total effective rate |
| Treatment group | 35 | 3 | 10 | 16 | 6 | 82.8％ |
Table 6 embodies soya-bean polypeptides nutrient solution of the present invention can reach 82.8% to the treatment total effective rate of gastric ulcer, and in addition, the inspection of every symptom and sign also embodies significant improvement.
4 soya-bean polypeptides nutrient solutions of the present invention are to the clinical observation on the therapeutic effect of chronic superficial gastritis
Diagnostic criteria and curative effect determinate standard are all formulated by " new Chinese medicine treatment chronic superficial gastritis guideline of clinical investigations " that the Ministry of Public Health develops and publishes.
[diagnostic criteria]: course of disease delay, in various degree indigestion, apocleisis, the nauseating and relevant symptoms such as upper abdomen pain of taking food are arranged, the left upper abdomen mild tenderness can be arranged;
[curative effect determinate standard]
1. produce effects: symptom and sign all improves more than 2 grades, gastroscope and pathologic finding, and inflammation obviously alleviates;
2. effective: symptom and sign all improves more than 1, gastroscope and pathologic finding, and inflammation alleviates;
3. invalid: the not obvious variation of symptom and sign does not reach effective standard or deterioration
[methods for the treatment of]:
60 routine experimenters are former, and to take treatment stomach trouble medication dosing kind constant, is divided at random the therapeutic combination control group, and every group of 30 examples, treatment group add the soya-bean polypeptides nutrient solution of the embodiment of the invention 1 preparation, every day 2 times, each 30ml; Control group does not add any health products or medicine.Took continuously 30 days.Respectively check symptom, sign, OGD and every physical and chemical index one time before and after the test, the result is as follows:
The total effective rate of table 7 soya-bean polypeptides nutrient solution treatment of the present invention chronic superficial gastritis
Table 7 as can be known, soya-bean polypeptides nutrient solution of the present invention is significantly higher than the control group of not taking this nutrient solution to the treatment total effective rate of chronic superficial gastritis, in addition, finds in the variation of gastric mucosa through gastric endoscopic examination, the improvement rate for the treatment of group can reach 35.7%, and control group only has 20.0%; Other symptom and signs also contrast group have clear improvement.
In sum, soya-bean polypeptides nutrient solution of the present invention all has good result for the treatment of to chronic hepatitis B, acne, gastric ulcer and chronic superficial gastritis.
Claims (8)
1. a soya-bean polypeptides nutrient solution is characterized in that, it is that described extracting method may further comprise the steps by the light yellow clarifying liquid body of physical method extraction from soybean:
1) electromagnetic dissociation: it is in the electromagnetic dissociation device of putting down in writing in the utility model patent specification of ZL200420059838 that soybean is entered the patent No. continuously, and this device comprises totally enclosed silo, material import and export, electromagnetic generator, spiral release cage device and condenser; Described material inlet is bucket shape, is arranged at the top of silo, and material outlet is tubular, is arranged at the below of silo, spiral is installed in it releases the cage device, and this spiral is released the cage device and driven by gearbox and buncher; Described electromagnetic generator comprises a high-frequency signal generating source, be fixed in the coniform electromagnetism anode of central authorities in the silo and form with three parts of silo as the electromagnetism negative electrode; Described condenser is connected with silo inside, and an axial flow blower is set at the condensator outlet place; The output frequency of described high-frequency signal generating source is 200KHZ to 2000MHZ, power output after material enters the electromagnetic dissociation device, adds thermal material to 100 ~ 110 ℃ in 100W to 100KW scope, collect the steam and the condensation that produce, obtain liquid I and solid material I;
2) squeezing lixiviate: solid material I oil meal after the routine oil expression is processed that step 1) is obtained separates, and the dregs of beans that obtains adds the water soaking of 3 times of weight, and stirs after 30 minutes under ultrasonic vibration and carry out Separation of Solid and Liquid, obtains solid material II and liquid II;
3) the liquid II standing demix that filtering and concentrating: with step 2) obtains obtains the supernatant on upper strata and the dirty solution of lower floor, and supernatant by the molecular sieve filtration of molecular weight below 2000, is leached liquid III; It is concentrated to heat after the liquid by molecular sieve and dirty solution do not mix, and collects the steam and the condensation that produce, until percent by weight of dry matter content 〉=30% in the mixed liquor, obtains congee shape solid material III and liquid IV after concentrated;
4) the solid material III that the solid material II that dry extracting: with step 2) obtains and step 3) obtain is dry the processing respectively, until wherein water content drops to below 10%, collects the steam and the condensation that produce therebetween and is mixed to get liquid V;
5) merge extract: the liquid V that the liquid I that step 1) is obtained, the liquid III that step 3) obtains and liquid IV and step 4) obtain mixes.
2. soya-bean polypeptides nutrient solution claimed in claim 1 is characterized in that: step 2) described oil expression processes and adopts screw oil expeller to finish; Described Separation of Solid and Liquid adopts disk centrifuge to finish.
3. soya-bean polypeptides nutrient solution claimed in claim 1 is characterized in that: the mixed liquor that step 5) obtains is further processed through ultraviolet disinfection.
4. the preparation method of soya-bean polypeptides nutrient solution claimed in claim 1 may further comprise the steps:
1) electromagnetic dissociation: it is in the electromagnetic dissociation device of putting down in writing in the utility model patent specification of ZL200420059838 that soybean is entered the patent No. continuously, and this device comprises totally enclosed silo, material import and export, electromagnetic generator, spiral release cage device and condenser; Described material inlet is bucket shape, is arranged at the top of silo, and material outlet is tubular, is arranged at the below of silo, spiral is installed in it releases the cage device, and this spiral is released the cage device and driven by gearbox and buncher; Described electromagnetic generator comprises a high-frequency signal generating source, be fixed in the coniform electromagnetism anode of central authorities in the silo and form with three parts of silo as the electromagnetism negative electrode; Described condenser is connected with silo inside, and an axial flow blower is set at the condensator outlet place; The output frequency of described high-frequency signal generating source is 200KHZ to 2000MHZ, and power output is in 100W to 100KW scope, after material enters the electromagnetic dissociation device, add thermal material to 100 ~ 110 ℃, collect the steam and the condensation that produce, obtain liquid I and solid material I;
2) squeezing lixiviate: solid material I oil meal after the routine oil expression is processed that step 1) is obtained separates, and the dregs of beans that obtains adds the water soaking of 3 times of weight, and stirs after 30 minutes under ultrasonic vibration and carry out Separation of Solid and Liquid, obtains solid material II and liquid II;
3) the liquid II standing demix that filtering and concentrating: with step 2) obtains obtains the supernatant on upper strata and the dirty solution of lower floor, and supernatant by the molecular sieve filtration of molecular weight below 2000, is leached liquid III; It is concentrated to heat after the liquid by molecular sieve and dirty solution do not mix, and collects the steam and the condensation that produce, until percent by weight of dry matter content 〉=30% in the mixed liquor, obtains congee shape solid material III and liquid IV after concentrated;
4) the solid material III that the solid material II that dry extracting: with step 2) obtains and step 3) obtain is dry the processing respectively, until wherein water content drops to below 10%, collects the steam and the condensation that produce therebetween and is mixed to get liquid V;
5) merge extract: the liquid V that the liquid I that step 1) is obtained, the liquid III that step 3) obtains and liquid IV and step 4) obtain mixes.
5. preparation method claimed in claim 4 is characterized in that: it is that the electromagnetic dissociation device of putting down in writing in the utility model patent specification of ZL200420059838 is finished that step 1) adopts the patent No..
6. preparation method claimed in claim 4 is characterized in that: step 2) described oil expression processes and adopts screw oil expeller to finish; Described Separation of Solid and Liquid adopts disk centrifuge to finish.
7. preparation method claimed in claim 4 is characterized in that: the mixed liquor that step 5) obtains is further processed through ultraviolet disinfection.
8. the application of soya-bean polypeptides nutrient solution claimed in claim 1 in the health food of preparation control chronic hepatitis B, acne, gastric ulcer or superficial gastritis.
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| CN102920803B (en) * | 2012-11-26 | 2013-11-20 | 李成仁 | Oil mixture capable of treating acne |
| CN104817622A (en) * | 2014-01-30 | 2015-08-05 | 陈光健 | Oligopeptide CD04, and preparation method and application thereof |
| CN104817625A (en) * | 2014-01-30 | 2015-08-05 | 陈光健 | Oligopeptide CD03, and preparation method and application thereof |
| CN104352551B (en) * | 2014-10-29 | 2018-04-20 | 孙震 | A kind of radix glycyrrhizae electromagnetic dissociation powder and its preparation method and application |
| CN108685096A (en) * | 2017-04-05 | 2018-10-23 | 杨绪奎 | A kind of soybean essence extracting solution liver-protecting product |
| CN109645480A (en) * | 2017-10-11 | 2019-04-19 | 山西鑫釜生物科技有限公司 | Application of the soybean essence extracting solution in preparation nourishing the stomach product |
| CN113621019A (en) * | 2021-07-10 | 2021-11-09 | 吉林省金鹿药业有限公司 | Method for extracting liquorice small-molecule peptide liquid and peptide powder by electromagnetic cracking technology |
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Inventor after: Sun Zhen Inventor after: Di Jingbo Inventor after: Sun Zhentian Inventor after: Gao Wenbin Inventor after: Zhang Xueji Inventor after: Ma Runlin Inventor after: Qi Lianwen Inventor after: Jiang Mozhu Inventor before: Sun Zhen Inventor before: Di Jingbo Inventor before: Sun Zhentian |
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