CN106973888A - A kind of preservation liquid of pathological tissue specimen - Google Patents

Abstract

The invention discloses a preservation solution for pathological tissue specimens, which is prepared by mixing the following raw materials according to the weight ratio: 2-5 parts of potassium sorbate, 10-20 parts of industrial ethanol, 1-3 parts of glycerin, and 2 parts of glutaraldehyde. ‑3 parts, 8.5‑9.5 parts of sodium chloride, 0.6‑1 part of nano silver, 10‑20 parts of citric acid, 60‑100 parts of water. The specimen preservation solution of the present invention is a colorless and transparent liquid, has no irritating smell, and has excellent sterilization and anti-mildew properties; it can effectively maintain the tissue shape and color of the original biological specimen, has no volatile substances, has a long-lasting effect, and can effectively reduce costs; And it does not contain toxic and harmful substances, which is green and environmentally friendly, and reduces the burden on the environment.

Description

Preservation solution for pathological tissue specimens

technical field

The invention relates to the technical field of pathological tissue preservation, in particular to a pathological tissue specimen preservation solution.

Background technique

From the operating room to the pathology department, laboratory surgery, tissue biopsy, histopathology department, gastroscopy department, etc., it is necessary to use specimen preservation solution for fixing, storing and retaining samples of tissues or cells. The existing preservation solution is mainly Fu formalin preservation solution. Formalin specimen preservation solution has a good fixation effect, which is due to the large amount of formaldehyde combined with the functional groups of the specimen protein, and after long-term storage, it further polymerizes to form paraformaldehyde. This process makes it form a moderate cross-link between protein peptide chains, which can not only kill bacteria, but also maintain the tissue shape of the specimen. However, formaldehyde and benzene have been identified as carcinogens by the World Health Organization and are listed as highly toxic chemicals, which can strongly irritate the eyes, skin, and mucous membranes of the respiratory tract, etc., and eventually cause immune dysfunction, liver damage, and nervous central system. It will lead to fetal malformation, leukemia, chronic respiratory diseases, nasopharyngeal cancer, female menstrual disorders, acute mental depression and other diseases, which pose a serious threat to human health. At the same time, due to the low boiling point of formalin, it is very volatile at room temperature. As the temperature rises, the volatilization speed is accelerated, and a strong pungent smell is emitted, which seriously pollutes the laboratory environment. As time goes by, the concentration of formaldehyde in the air continues to increase, which poses a serious threat to the health of medical, biological and zoological workers. In addition, the specimens preserved in formaldehyde solution for a long time may become mildewed, yellowed, and hardened.

Invention content:

The object of the present invention is to provide a preservation solution for pathological tissue samples. The preservation solution does not contain formaldehyde and formalin, volatilizes slowly, has no irritating smell, has low toxicity, high safety, does not pollute the experimental environment, has good preservation effect, and will not go moldy when preserved for a long time. The texture is soft and the color is good.

In order to solve the above-mentioned technical problems, the present invention adopts the following technical solutions to realize:

A preservation solution for pathological tissue specimens, which is prepared by mixing the following raw materials in proportions by weight:

2-5 parts of potassium sorbate, 10-20 parts of industrial ethanol, 1-3 parts of glycerin, 2-3 parts of glutaraldehyde, 8.5-9.5 parts of sodium chloride, 0.6-1 part of nano silver, 10-20 parts of citric acid , 60-100 parts of water.

Preferably, the preservation solution of the above-mentioned pathological tissue specimen is prepared by mixing the following raw materials in proportions by weight:

Potassium sorbate 2 parts, industrial ethanol 10 parts, glycerin 1 part, glutaraldehyde 2 parts, sodium chloride 8.5 parts, nano silver 0.6 parts, citric acid 10 parts, water 60 parts.

Preferably, the preservation solution of the above-mentioned pathological tissue specimen is prepared by mixing the following raw materials in proportions by weight:

Potassium sorbate 3.5 parts, industrial ethanol 15 parts, glycerin 2 parts, glutaraldehyde 2.5 parts, sodium chloride 9 parts, nano silver 0.8 parts, citric acid 15 parts, water 80 parts.

Preferably, the preservation solution of the above-mentioned pathological tissue specimen is prepared by mixing the following raw materials in proportions by weight:

Potassium sorbate 5 parts, industrial ethanol 20 parts, glycerin 3 parts, glutaraldehyde 3 parts, sodium chloride 9.5 parts, nano silver 1 part, citric acid 20 parts, water 100 parts.

The mass fraction of the glutaraldehyde is 50-52%.

The potassium sorbate is a colorless scaly crystalline powder, odorless and tasteless, and easily soluble in water and ethanol. Potassium sorbate has high-efficiency antiseptic and antibacterial effects, and has good thermal stability, decomposition temperature as high as 270°C, and low toxicity. It has obvious inhibitory effect on enzyme bacteria, yeast, and aerobic microorganisms. The optimum pH value for antibacterial is 5.0-6.0, and its antibacterial effect is 5-10 times higher than that of benzoic acid, and its toxicity is only five times that of benzoic acid. It has no adverse effect on the flavor of food, and is widely used in antiseptic preservation of meat, fish, eggs, poultry products, wine, fruit wine, dairy products, etc. For the preservation of human specimens, it has the functions of anticorrosion, sterilization, maintaining the color and humidity of the original biological specimen tissue, and preventing mildew.

The nano-silver is a simple metal silver whose particle size is processed to a nanometer level. Its particle size is generally 2-15nm. It is a new, broad-spectrum, safe, and strong antibacterial activity nano-inorganic antibacterial material that has been widely studied and applied in recent years. Drugs are incomparable. At present, as a new type of antibacterial agent, it has been widely used in the fields of medical treatment and building materials. Its surface is closely combined with protein molecules through nitrogen atoms, which can moderately cross-link the protein of human specimen tissue, and then play a role in fixing, maintaining the original shape of human specimen and the softness and toughness of the original tissue.

The citric acid is a non-toxic and tasteless organic acid, which has strong bactericidal and antioxidative effects, can effectively kill bacterial spores polluted in the pipeline of the hemodialysis machine, and has high-efficiency bacteriostasis and biological preservation for the preservation of biological specimens. The role of the original color of the specimen is beneficial to the preparation of the biological specimen preservation solution.

Compared with the prior art, the present invention has the advantages of:

1. The specimen preservation solution of the present invention is a colorless and transparent liquid, has no pungent smell, and has excellent bactericidal and anti-mildew properties;

2. The specimen preservation solution of the present invention can effectively maintain the tissue shape and color of the original biological specimen, has no volatile substances, has a long-lasting effect, and can effectively reduce costs;

3. The novel specimen preservation solution of the present invention does not contain toxic and harmful substances, is green and environmentally friendly, and reduces the burden on the environment.

detailed description

Below adopt specific embodiment to further illustrate content of the present invention.

The following content is a further detailed description of the present invention in conjunction with specific preferred embodiments, and it cannot be assumed that the specific implementation of the present invention is limited to these descriptions. For those of ordinary skill in the technical field of the present invention, without departing from the concept of the present invention, some simple deduction or replacement can be made, which should be regarded as belonging to the protection scope of the present invention.

Example 1

A preservation solution for pathological tissue specimens, which is prepared by mixing the following raw materials in proportions by weight:

Potassium sorbate 2g, industrial ethanol 10g, glycerin 1g, glutaraldehyde 2g, sodium chloride 8.5g, nano silver 0.6g, citric acid 10g, water 60g.

The mass fraction of glutaraldehyde is 50%.

Example 2

A preservation solution for pathological tissue specimens, which is prepared by mixing the following raw materials in proportions by weight:

Potassium sorbate 3.5g, industrial ethanol 15g, glycerin 2 parts, glutaraldehyde 2.5g, sodium chloride 9g, nano silver 0.8g, citric acid 15g, water 80g.

The mass fraction of glutaraldehyde is 51%.

Example 3

A preservation solution for pathological tissue specimens, which is prepared by mixing the following raw materials in proportions by weight:

Potassium sorbate 5g, industrial ethanol 20g, glycerin 3g, glutaraldehyde 3g, sodium chloride 9.5g, nano silver 1g, citric acid 20g, water 100g.

The mass fraction of glutaraldehyde is 52%.

Claims (5)

1. A preservation solution for pathological tissue specimens is characterized in that: it is made by mixing the following raw materials in proportions by weight: 2-5 parts of potassium sorbate, 10-20 parts of industrial ethanol, 1-3 parts of glycerin, 2-3 parts of glutaraldehyde, 8.5-9.5 parts of sodium chloride, 0.6-1 part of nano silver, 10-20 parts of citric acid , 60-100 parts of water. 2. The preservation solution of pathological tissue specimens according to claim 1, characterized in that: it is made by mixing the following raw materials in proportions by weight: Potassium sorbate 2 parts, industrial ethanol 10 parts, glycerin 1 part, glutaraldehyde 2 parts, sodium chloride 8.5 parts, nano silver 0.6 parts, citric acid 10 parts, water 60 parts. 3. The preservation solution of pathological tissue specimen according to claim 1, characterized in that: it is made by mixing the following raw materials in proportions by weight: Potassium sorbate 3.5 parts, industrial ethanol 15 parts, glycerin 2 parts, glutaraldehyde 2.5 parts, sodium chloride 9 parts, nano silver 0.8 parts, citric acid 15 parts, water 80 parts. 4. The preservation solution of pathological tissue specimens according to claim 1, characterized in that: it is made by mixing the following raw materials in proportions by weight: Potassium sorbate 5 parts, industrial ethanol 20 parts, glycerin 3 parts, glutaraldehyde 3 parts, sodium chloride 9.5 parts, nano silver 1 part, citric acid 20 parts, water 100 parts. 5. The preservation solution of pathological tissue specimens according to claim 1, characterized in that: the mass fraction of glutaraldehyde is 50-52%.