CN104186481B - A kind of concave convex rod compound chlorhexidine-containing disinfectant and preparation method thereof - Google Patents

Abstract

The invention discloses an attapulgite composite chlorine disinfectant, which is composed of attapulgite and chlorine complex, wherein the attapulgite is used as a slow-release adsorbent, and the chlorine complex is adsorbed on the specific surface. The attapulgite compound chlorine disinfectant provided by the present invention not only has the characteristics of a general disinfectant, but also has the functions of effectively absorbing peculiar smells and can be used for disinfection of the environment, instruments and fruits, and can also be toxic to animals. It can be used for disinfection of animal food, feed and drinking swill, without affecting the nutritional value of the disinfected object, non-irritating, safe and reliable. Moreover, it has no toxic and side effects, does not cause any pollution to the environment, and is green and environmentally friendly. According to the detection result to the product of the present invention, this product all has significant killing effect to pathogenic microorganisms such as virus bacteria. The product solution can kill 100% pathogenic microorganisms.

Description

A kind of attapulgite compound chlorine disinfectant and preparation method thereof

technical field

The invention relates to a chlorine-containing compound disinfectant.

Background technique

Chlorine-containing disinfectant is one of the earliest chemical disinfectants used in the world. It refers to a disinfectant that produces hypochlorous acid when it meets water. It is cheap, low in toxicity, and plays an important role in reducing pathogenic microorganism contamination and preventing infection, so it is widely used. It is one of the most widely used disinfectants at present. Chlorine-containing disinfectants are divided into inorganic compounds and organic compounds. Inorganic mainly includes chlorine gas, bleaching powder, chlorine dioxide, and sodium hypochlorite; organic mainly includes: dichloroisohydrouric acid, sodium dichloroisohydrouric acid, sodium trichloroisohydrouric acid, dichlorohydantoin, bromochlorohydantoin, etc. The former is mainly hypochlorous acid, which has a fast sterilization speed, but its property is unstable; the latter is mainly composed of chloramine, which is stable in nature, but its sterilization speed is slow. The general mechanism of sterilization is: (1) the formed hypochlorous acid acts on the bacterial protein; (2) the hypochlorous acid decomposes to form new ecological oxygen and the strong oxidizing ability of chlorine dioxide, which oxidizes the bacterial protein; (3) disinfection The available chlorine contained in the agent will directly act on the cell protein. Therefore, the effect of the disinfectant is mostly proportional to the content of available chlorine, and the available chlorine determines the strength of the bactericidal effect of the chlorine-containing disinfectant. Chlorine disinfectants kill almost all types of microorganisms, even some bacterial spores and viruses. However, chlorine-containing disinfectants also have their own weaknesses. Its bactericidal performance is easily affected by organic matter and pH, and has a certain corrosive effect on items. Some chlorine-containing disinfectants (such as chlorine dioxide, etc.) are not stable enough, easy to decompose, and disinfect Agents can also react with aromatic compounds in the system to generate highly toxic substances. Most high-efficiency disinfectants are highly corrosive and irritating, and destructive and damaging to environmental objects and fresh plants. Therefore, it is very important to select a safe and non-destructive disinfectant that can effectively disinfect the ambient air and the surfaces of animals and plants to eliminate the spread of pollution and protect the environment from damage.

Contents of the invention

The technical problem to be solved by the present invention is to overcome the existing defects, and to provide a sterilizing agent that not only retains a good bactericidal effect, but also achieves small pungent odor, stable performance, and low toxicity, and is suitable for stainless steel, carbon steel, copper, and aluminum. Non-corrosive, attapulgite composite chlorine disinfectant with good applicability.

The purpose of the present invention is specifically achieved through the following technical solutions:

An attapulgite composite chlorine disinfectant is composed of attapulgite and chlorine complex, wherein the attapulgite is used as a slow-release adsorbent, and the chlorine complex is adsorbed on the specific surface.

Preferably, the chlorine complex is sodium dichloroisocyanurate or trichloroisocyanuric acid.

As a further preferred solution, the above-mentioned attapulgite composite chlorine disinfectant has the following components and parts by weight: 60-80 parts of attapulgite and 20-40 parts of sodium dichloroisocyanurate.

As the best solution, the above-mentioned attapulgite compound chlorine disinfectant has the following components and parts by weight: 60 parts of attapulgite and 40 parts of sodium dichloroisocyanurate.

The preparation method of the above-mentioned attapulgite composite chlorine disinfectant comprises drying and pulverizing the attapulgite and sodium dichloroisocyanurate respectively, and then mixing them in proportion.

Preferably, the dried and pulverized raw materials are sieved through a 100-mesh sieve and then mixed.

Principle of the present invention:

Attapulgite clay refers to a clay mineral with attapulgite as the main component. Attapulgite is a crystalline hydrated magnesium aluminum silicate mineral with a unique layered chain structure. There are lattice replacements in its structure, and the crystals contain variable amounts of Na ⁺ , Ca ²⁺ , Fe ^{3 +} , Al ³⁺ , the crystals are needle-like, fibrous or fibrous aggregates. Attapulgite has good colloidal properties such as unique dispersion, high temperature resistance, salt and alkali resistance, and high adsorption capacity. Due to the unique crystal structure of attapulgite, it has many special physical, chemical and technological properties. The main physical and chemical properties and technological properties are: cation exchangeability, adsorption, adsorption decolorization, large specific surface area (9.6 ~ 36m2/g), colloidal value and expansion capacity. These properties can prolong the contact time of the disinfection object, and greatly reduce the corrosion resistance to metals, etc., and its stability is also greatly enhanced. Therefore, the effectiveness of its sterilization is improved. With the development of automatic cleaning systems, non-corrosive, slow-release, and high-efficiency fungicides have become ideal objects of use. The present invention uses attapulgite as the slow-release adsorbent, and absorbs the disinfectant containing organic chlorine in the pores. After reasonable formula and manufacturing process, it not only retains good bactericidal effect, but also realizes small pungent smell and stable performance. , Low toxicity, no corrosion requirements for stainless steel, carbon steel, copper, aluminum, and good applicability. Because its mineral deposits are mainly in western regions such as Gansu, there is no real systematic research, development and utilization by pharmaceutical companies. Therefore, it is not convenient to use the mine in the eastern region, and the pharmaceutical industry in the western region is not developed enough, so the application of the mine is currently limited. In fields such as medicine, no one studies and develops attapulgite. After long-term and in-depth research on attapulgite in this application, the ingenious application of attapulgite as a carrier of chlorine disinfectant not only strengthens the disinfection effect of chlorine disinfectant, but also avoids many defects of chlorine disinfectant, resulting in unexpected Unexpected technical effects.

Beneficial effects of the present invention:

The attapulgite compound chlorine disinfectant provided by the present invention not only has the characteristics of a general disinfectant, but also has the functions of effectively absorbing peculiar smells and can be used for disinfection of the environment, instruments and fruits, and can also be toxic to animals. It can be used for disinfection of animal food, feed and drinking swill, without affecting the nutritional value of the disinfected object, non-irritating, safe and reliable. Moreover, it has no toxic and side effects, does not cause any pollution to the environment, and is green and environmentally friendly. According to the detection result to the product of the present invention, this product all has significant killing effect to pathogenic microorganisms such as virus bacteria. The product solution can kill 100% pathogenic microorganisms.

In order to further illustrate the essence of the present invention, the applicant has done the attapulgite composite chlorine disinfectant effect test as follows.

The applicant has carried out the antibacterial (inactivation) test (M.I.C), low-temperature antibacterial test and safety test on two batches of "Attapulgite Composite Chlorine Disinfectant" (different proportions), and now Report the results below:

1. Test materials

1. Drugs:

Test drug: "Attapulgite Compound Chlorine Disinfectant" I (sodium dichloroisocyanurate 40%, attapulgite 60%) and II (sodium dichloroisocyanurate 20%, attapulgite 80%), white powder , in bulk (tablets).

Control drug: "Qiangliduling", produced by Chinese Veterinary Medicine Factory of Chinese Academy of Agricultural Sciences, white powder (composed of 22% sodium dichloroisocyanurate, 8% sodium tripolyphosphate, and 70% sodium sulfate), packed in plastic cans.

2. Bacteria (poison) species to be tested:

(1) Bacteria:

All the above strains were selected from multiple strains of the same genus and species through resuscitation, morphological characteristics, cultural characteristics, systemic physiological and biochemical reactions and antigen identification, and were screened by inoculating experimental animals, which proved to have the genus and species. characteristics and better pathogenic strains.

(2) Viruses:

Chicken Newcastle Disease Virus: Freeze-dried and virulent F48E8, provided by the Central Prison

Classical swine fever virus: freeze-dried virus F486 attenuated rabbit virus, provided by the Central Prison

The above-mentioned poisonous species are used in the test after passing through the corresponding experimental animals and passing the determination of the poisonous price.

3. Experimental animals, medium and reagents:

animal:

Mice: about 20 grams of healthy Kunming mice;

Rabbit: 1.8-2.0KG healthy rabbit;

Chicken: 12-month-old non-immune healthy chicken;

Chicken embryos: 9-day-old healthy and live chicken embryos.

Medium:

Nutrient Broth, Martin Broth, Standard Agar Plate, Martin Agar Plate, KM Mycoplasma Medium, etc.

(3) Thinner:

PBS, sterile saline, deionized water.

2. Method

1. All test methods and result observations and judgments are carried out with reference to reference materials 1-7 and related materials (omitted).

2. Various test conditions are determined according to the purpose of the test: the test drug has an antibacterial effect time of 1-2 minutes, and a virus inactivation time of 8-10 minutes; In "Bacteria Test", the sensible temperature is -4±3°C, and the rest are at room temperature (25±4°C).

3. Test results

Bacteriostatic effect (M.I.C): On the basis of the preliminary test, five positive tests were carried out, and then analysis and final judgment were carried out. The results are shown in Table 1.

Table 1 "M.I.C" final result and comparison of "Attapulgite Compound Chlorine Disinfectant" on each bacterial (virus) strain

2. Determination of the growth prevention rate of bacteria by "Attapulgite Composite Chlorine Disinfectant":

On the basis of the minimum inhibitory dose (M.I.C) detection of bacteria, according to reference material 5 and related methods, the results are shown in Table 2.

Table 2 "Attapulgite Compound Chlorine Disinfectant" Determination of Bacterial Growth Rate Determination Results

Note: The number before the brackets in the table is the number of bacterial growth, and the number in the brackets is the growth arrest rate of bacteria (%)

3. Low-temperature antibacterial test: Under low temperature conditions (-4±3°C), a total of three antibacterial tests were carried out on each bacterial strain. The results are shown in Table 3.

table 3:

Note: "—" indicates that the bacteria are inhibited; "+" indicates that the bacteria are not inhibited; Ⅰ, Ⅱ indicate the batch number of "citric acid compound disinfectant".

4. Virus inactivation test:

See Table 4 and Table 5 for the inactivation effects on Newcastle disease virus and classical swine fever virus respectively.

Table 4 to the inactivation effect of chicken Newcastle disease virus

Table 5 to the inactivation effect of swine fever virus

5. Safety tests on animals:

a. Oral test: 3 test rabbits were used, and an average of 375ml of "Attapulgite Compound Disinfectant" was taken per day, that is, the concentrated solution of Attapulgite Compound Chlorine Disinfectant Ⅰ diluted by 1: (250-400), and even served 7 days. Results: No obvious symptoms and substantive lesions of tissues and organs were found in clinical and autopsy.

b. Skin and mucous membrane test: Take 3 test rabbits, and apply "Attapulgite Compound Chlorine Disinfectant Ⅰ" Ⅰ with 1:100 dilution every day to the depilatory area of their abdomen (the area is about 15cm ² ). Instill 2-3 drops of the diluted solution in one eye for 7 days. Results: No obvious inflammatory reaction was found in the skin and mucous membrane.

c. Intravenous injection test: 18 experimental rabbits were randomly divided into 6 groups, 3 in each group, and the rabbits in each group were injected into the ear vein in order of 40mg/rat, 20mg/rat, 10mg/rat, 5mg/rat, 2.5mg/rat. / only, 1.25mg / only. Continuous observation for 7 days. Results: There were no obvious clinical symptoms and organic lesions in tissues and organs in each group of rabbits.

Four. Summary

1. The test was precisely designed, and the test drug "attapulgite compound chlorine disinfectant" was tested in a wide range, and the formal test was repeated many times, so the accuracy of the results is high. In the antibacterial test of bacteria, the number of bacteria is controlled in a consistent and specific amount; in the virus inactivation test, the amount of the virus inoculated is 2-5 times that of the standard virus inoculated, which further strengthens the identification of its results reliability.

2 The test results show that the antibacterial effect of "attapulgite compound chlorine disinfectant" Ⅰ is slightly higher than that of "attapulgite compound chlorine disinfectant" Ⅱ. The minimum inhibitory dose (M.I.C) of "Attapulgite Composite Chlorine Disinfectant" Ⅰ to Staphylococcus aureus and Escherichia coli is three times that of "Qianqiang Disinfectant", and the minimum inhibitory dose (M.I.C) to the other 4 kinds of bacteria is both It is 1.5 times that of "Qianli Disinfectant", and its inhibitory effect on both viruses is 6 times that of "Qianli Disinfectant". The antibacterial effect at low temperature is also significantly better than that of "Qianli Disinfectant". This shows that "attapulgite compound chlorine disinfectant Ⅰ" Ⅰ has a good effect of inhibiting bacteria and viruses.

3 "Attapulgite Composite Chlorine Disinfectant Ⅰ" Ⅰ has significantly better inactivation effects on the two viruses than "Qiangliduling"; "Attapulgate Composite Chlorine Disinfectant" Ⅰ also has very good safety test results on animals. This further increases the safety and reliability of the "Attapulgite Composite Chlorine Disinfectant" I in practical applications.

Based on the above test results and analysis, it is proved that "attapulgite compound chlorine disinfectant Ⅰ" is the best dosage form; "attapulgite compound chlorine disinfectant Ⅰ" is an efficient, broad-spectrum, strong, safe It is a new type of disinfectant that can be widely used in various environments, livestock and poultry body surfaces, pens, aquatic products (such as fish ponds), production equipment, forage feed, various utensils, drinking water, feces, etc. , medical and public health and other aspects have good value and broad application prospects.

detailed description

Preferred embodiments of the present invention are described below, and it should be understood that the preferred embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

Example 1:

Use attapulgite compound chlorine disinfectant Ⅰ (diluted 1:20000), the stable disinfectant solution is sprayed and wetted on the surface of the object, and it acts for 4 minutes. Using the same concentration of attapulgite compound chlorine disinfectant Ⅰ to soak fresh cucumber for 4 minutes, the kill logarithms of artificially polluted Escherichia coli were all >3.00. Soak in the attapulgite composite chlorine disinfectant Ⅰ (diluted 1:10000) solution for 4 minutes, and the kill logarithms of Escherichia coli artificially contaminated on porcelain bowls and bamboo chopsticks were all >3.00.

Example 2:

Use attapulgite compound chlorine disinfectant Ⅰ (diluted 1:18000), the compound chlorine-containing disinfectant acts for more than 8 minutes, and has a good killing effect on the surface of environmental objects, fruits and vegetables, and natural bacteria on the surface of growing plants. Under the condition of the highest test concentration, spraying directly on the leaves of plants and the surface of flowers did not cause any damage. Visually, there was no difference in growth and appearance between the sprayed disinfectant group and the unsprayed control group. After spraying and moistening the leaves of 4 kinds of growing plants and the surfaces of 2 kinds of open flowers with aqueous solution of chlorine-containing disinfectant, they were observed continuously for 3 days. Compared with those without spraying disinfectant, no effect on plant growth was found.

Example 3:

Soak the rusted oral metal instruments with the aqueous solution of Attapulgite Composite Chlorine Disinfectant Ⅰ (diluted 1:5000) for 15 days, and no aggravation of rust is seen; continuous immersion of rubber products and cloth for 15 days also shows no change. Metal medical instruments were continuously soaked in an aqueous solution of attapulgite compound chlorine disinfectant Ⅰ (diluted 1:5000) for 15 days, and no rust and corrosion was found; after soaking metal medical instruments with the same concentration of 84 disinfectant for 24 hours, rust began to appear, continuous Determination results of available chlorine content in use. It was determined that the aqueous solution of Attapulgite Compound Chlorine Disinfectant Ⅰ (diluted 1:18000 and 9000 times) continuously soaked instruments until the 12th day, and the effective chlorine content decreased by 0.30% and 0.36%, respectively; the pH value decreased slightly with the extension of use , but the decrease is not obvious.

Example 4:

In the experiment on the killing effect of Vibrio in river water, the aqueous solution of attapulgite compound chlorine disinfectant I (diluted 180000, 135000, 90000 times) was used, and the action time was 6 hours, 3 hours and 1 hour respectively; the attapulgite compound chlorine disinfectant Ⅰ (diluted 1,800,000 and 900,000 times) aqueous solution, the action time is 4 hours and 2 hours respectively; the aqueous solution of Attapulgite Composite Chlorine Disinfectant Ⅰ (diluted 450,000 times), the action time is only 1 hour. The organic content in hospital sewage is high, the concentration required for sterilization is also high, and the action time is prolonged; the river water is clear and transparent, the amount of polluted organic matter is less, and the concentration required for sterilization is also lower.

Example 5:

Use an aqueous solution of attapulgite compound chlorine disinfectant Ⅰ (diluted 1:10000), and the disinfectant acts for 5 minutes, and the average killing logarithm of the poliovirus in the suspension is >4.00.

The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still understand the foregoing embodiments The recorded technical solutions are modified, or some of the technical features are equivalently replaced. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the protection scope of the present invention.

Claims (3)

1. a concave convex rod compound chlorhexidine-containing disinfectant, it is characterized in that: be made up of attapulgite and chloro-complex, wherein, attapulgite is as slowly-releasing adsorbent, chloro-complex is adsorbed in specific surface, and each component and weight portion are: attapulgite 60 parts, sodium dichloro cyanurate 40 parts.

2. the preparation method of concave convex rod compound chlorhexidine-containing disinfectant according to claim 1, is characterized in that: attapulgite and sodium dichloro cyanurate are carried out drying, pulverization process respectively, then compatibility mixing in proportion.

3. the preparation method of concave convex rod compound chlorhexidine-containing disinfectant according to claim 2, is characterized in that: carried out mixing after 100 orders sieve by the raw material after drying, pulverizing again.