CN106442962A - POCT instant detection device - Google Patents
POCT instant detection device Download PDFInfo
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- CN106442962A CN106442962A CN201610819201.3A CN201610819201A CN106442962A CN 106442962 A CN106442962 A CN 106442962A CN 201610819201 A CN201610819201 A CN 201610819201A CN 106442962 A CN106442962 A CN 106442962A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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Abstract
The invention discloses a POCT instant detection device. The POCT instant detection device comprises a cover plate and a base plate, wherein the base plate comprises a first surface; a sample cell, a first groove, a reagent cell, a second groove, a waste liquid cell and a third groove are sequentially formed in the first surface; the base plate is tightly fit to the cover plate through the first surface; a through sample adding hole is formed in the position, corresponding to the sample cell, of the cover plate; a blood cell filter net is arranged in a first channel; at least one detection point is arranged in the second channel and is formed by antibody molecules coating the inner wall of the second channel; and fluorescence labeling antibodies corresponding to the antibody molecules are contained in the reagent cell. The POCT instant detection device can be used for detecting whole blood and is driven by power supplied by negative pressure connected to a third channel, and the whole blood enters a detection region after flowing through the blood cell filter net and does not need to be independently treated, so that the operation is rapid and convenient, and the result is accurate.
Description
Technical field
The invention belongs to immunoassay detection technique field, more particularly to a kind of POCT test and analyze device immediately.
Background technology
POCT (point of care testing) is referred to as instant detection technique, refers to the clinic for carrying out beside patient
Detection (also referred to as bedside detection-bed side testing), is generally carried out by Clinical Laboratory doctor, and in sampling location is
Quarter is analyzed, and saves complex process program of the sample when experiment pool is checked, while the class for quickly obtaining assay is new
Detection mode, this detection mode is one of field with fastest developing speed in current in-vitro diagnosis industry.In short, the master of POCT
It is the detection place that need not fix to want index, and reagent and instrument are all portable, and can be operated in time.
In the market POCT product typically the related liquid reagent in traditional method is infiltrated on filter paper and various
In the absorbent material of microporous membrane, become the dried reagent block of integration, be then fixed in hard type substrate, become various shapes
The diagnostic reagent srip of formula.In detection, detected using the capillarity of diagnostic reagent srip, the filter paper used in test strips
With the micropore offer capillary force in microporous membrane.However, as the micropore size size in filter paper and film and the uniformity are poor,
Filter paper and film are not uniform enough to the binding ability of reagent so that migration velocity of the reagent in filter paper and film is not easy to control, this
Detection results can all be affected a bit, particularly affect the repeatability of product.This is also that the diagnostic reagent of test strips class is difficult to make calmly
The reason for volume production product.
Content of the invention
It is an object of the invention to provide a kind of instant detection means of POCT, to realize accurately detecting immediately.
For achieving the above object, the present invention is employed the following technical solutions：
A kind of instant detection means of POCT, the detection means includes cover plate and base plate；
The base plate includes first surface, be sequentially provided with the first surface sample cell, first groove, reagent,
Two grooves, waste liquid pool and the 3rd groove, the sample cell is connected with the reagent by first groove, and the reagent passes through
Second groove is connected with the waste liquid pool, and the waste liquid pool is connected to the border of the first surface by the 3rd groove；
The base plate is fitted tightly with the cover plate by first surface so that the cover plate and first groove, second
First passage, second channel and third channel are formed respectively between groove and the 3rd groove, the waste liquid pool passes through third channel
With atmosphere；The cover plate is provided with the adding mouth of insertion in position corresponding with the sample cell；
Hemocyte drainage screen is provided with the first passage；At least one test point is provided with the second channel, described
At least one test point formed by the antibody molecule being coated on second channel inwall respectively；Include in the reagent with
The corresponding fluorescent-labeled antibody of the antibody molecule.
Detection means according to the present invention, it is preferable that the first passage, second channel and third channel are separately
For linear type or Uncrossed shaped form.
Detection means according to the present invention, it is preferable that the hemocyte drainage screen is logical described first by draw-in groove cuttage
In road.
Detection means according to the present invention, it is preferable that the test point is prepared in the second channel by point sample method
Bottom, the fluorescently-labeled antibody is sprayed on the bottom of the reagent by spraying process.
Detection means according to the present invention, it is preferable that the sample cell, first groove, reagent, second groove, waste liquid
The bottom of pond and the 3rd groove is concordantly arranged.
Detection means according to the present invention, it is preferable that the volume of the waste liquid pool is more than sample cell, the appearance of the sample cell
Product is more than reagent.
Detection means according to the present invention, it is preferable that the first surface is provided with locating dowel, the cover plate is provided with and
The location hole that locating dowel on one surface matches.
Detection means according to the present invention, it is preferable that the width of the first groove, second groove and the 3rd groove is
300-5000 μm, depth is 300-5000 μm.
Detection means according to the present invention, it is preferable that the material of the cover plate and base plate is selected from Merlon, poly dimethyl
Siloxanes, polystyrene and lucite.
Detection means according to the present invention, it is preferable that the detection means also includes negative port, the negative port
One end is connected with the third channel, and the other end is extended to outside the border of the base plate and cover plate.
Compared with prior art, the present invention has advantages below：
1) detection means of the present invention can adopt whole blood test, provide driving force drive by the negative pressure for being connected to third channel
Dynamic, detection zone being entered after hemocyte drainage screen, individually whole blood need not be processed again, not only convenient and swift, and
Detection by quantitative can be carried out, as a result accurately；
2) multiple determination point can be set, while carrying out the detection of many index in second channel；
3) detection means of the present invention can be detected using fluorescent immune method, and detection time is only 5-30min, carries significantly
High detection efficiency；
4) whole device of the present invention is simple to operate, directly can detect detection means insertion detecting instrument, not
Actuating device is needed, so as to be also beneficial to reduce the volume of detecting instrument；
5) this device can be processed using Mechanical Method, be easy to industrialized mass production.
Description of the drawings
Fig. 1 is a kind of schematic diagram of embodiment of the detection means of the present invention；
Fig. 2 is the schematic diagram of base plate in Fig. 1；
Fig. 3 is the schematic diagram of Fig. 1 cover plate.
Specific embodiment
Below in conjunction with accompanying drawing, the present invention is described in detail, but the present invention is not limited to this.
As Figure 1-3, what the detection means of the present invention included cover plate 2 and base plate 1, the cover plate 2 and base plate 1 prepares material
Matter can be selected from Merlon, polydimethylsiloxane, polystyrene and lucite etc.；Wherein, the base plate 1 includes first
Surface 10, is sequentially provided with sample cell 11, first groove 12, reagent 13, second groove 14, waste liquid pool on the first surface 10
15 and the 3rd groove 16, the sample cell 11 is connected with the reagent 13 by first groove 12, and the reagent 13 passes through
Second groove 14 is connected with the waste liquid pool 15, and the waste liquid pool 15 is connected to the first surface 10 by the 3rd groove 16
Border.In one embodiment, it is preferable that the sample cell 11, first groove 12, reagent 13, second groove 14, waste liquid pool 15
And the 3rd the bottom of groove 16 concordantly arrange, so as to be conducive to the abundant flowing of sample；It is further preferred that first ditch
The width of groove 12, second groove 14 and the 3rd groove 16 is 300-5000 μm, such as 1000,2000,3000 or 4000 μm, and which is deep
Spend for 300-5000 μm, such as 1000,2000,3000 or 4000 μm, excessive be unfavorable for reducing amount of samples, and too small be unfavorable for
Device is prepared and is operated.
The base plate 1 is fitted tightly with the cover plate 2 by first surface 10, so that the cover plate 2 and first groove
12nd, between second groove 14 and the 3rd groove 16, first passage, second channel and third channel are formed respectively.In the present invention,
Described fit tightly refer to first surface 10 with the joint place of the cover plate 2 in do not allow to exist and be enough to affect detection means
The material flowing of testing result, such as under understanding state, except sample cell 11, first between the first surface 10 and cover plate 2
Outside passage, reagent 13, second channel, waste liquid pool 15 and third channel, remaining place of first surface 10 is due to conforming to lid
Plate 2, and there is no material stream and move.In one embodiment, the first surface 10 is plane, and the cover plate 2 is flat board, so as to
In fitting tightly；Certainly, it will be appreciated by those skilled in the art that in other situations, the first surface 10 can also be with described
The on-plane surface that the contact surface of cover plate 2 matches, such as curved surface.In one embodiment, the first surface 10 is provided with positioning
Post 17, the cover plate 2 is provided with the location hole 22 for matching with the locating dowel 17, assembles in order to device, while also favourable
In fitting tightly for base plate 1 and cover plate 2.
The cover plate 2 is provided with the adding mouth 21 of insertion in position corresponding with the sample cell 11, in order to the sample
Sample is added in product pond 11；The waste liquid pool 15 passes through third channel and atmosphere, so that sample is easy to by sample cell
11 flow to waste liquid pool 15.
In the present invention, the shape of each passage can be multiple, there is no interference each other, and such as described first leads to
Road, second channel and third channel can be separately linear type or Uncrossed shaped form.Set in the first passage
There is hemocyte drainage screen 18, to carry out hemocyte filtration, the hemocyte drainage screen to the blood sample from sample cell 11
18 can be that whole blood commonly used in the art considers blood film or whole blood seperation film, and which can be cut into suitable shape to be arranged at the
In one passage, preferably by draw-in groove cuttage in the first passage；It will be appreciated by those skilled in the art that its particular location can be
The centre of first passage or two ends, preferably such as first passage and the junction of sample cell 11, reduce resistance, improve detection effect
Really.
Be provided with least one test point (not shown) in the second channel, such as 2-6 test point, described extremely
A few test point is formed by the antibody molecule being coated on second channel inwall respectively, is specifically coated mode for this area institute
Know, preferably prepared in the bottom of the second channel by the method for point sample；Include and the antibody in the reagent 13
The corresponding fluorescent-labeled antibody of molecule, it will be appreciated by those skilled in the art that the fluorescent labeling can be painted on reagent
In 13, it is also possible to be sprayed onto on basic material (as glass fibre) first, then basic material be cut into suitable size and insert examination
Agent pond 13；Preferably, the fluorescently-labeled antibody is sprayed on the bottom of the reagent 13 by spraying process.At one preferably
Embodiment, the volume of the waste liquid pool 15 is more than sample cell 11, and the volume of the sample cell 11 is more than reagent 13, so as to protect
Sample leakage is desirably prevented while card Detection results.
Blood sample is directly added sample cell 11 in detection by the detection means of the present invention, big in third channel connection
One end of gas provides negative pressure, and control sample is flowed from sample cell 11 to waste liquid pool 15, and wherein, sample is first by first passage
Hemocyte drainage screen 18 carry out hemocyte filtration, sample after filtration is entered to be carried out instead with fluorescent-labeled antibody in reagent 13
The coated antibody response of test point in second channel, with second channel should be entered afterwards, after the completion of reaction, be combined using instrument and exempt from
Epidemic disease Fluorometric assay signal simultaneously obtains testing result, and remaining sample enters waste liquid pool 15.
In an embodiment of the invention, the detection means also includes negative port (not shown), described
One end of negative port is connected with the third channel, and the other end is extended to outside the border of the base plate 1 and cover plate 2, in order to
It is connected with negative pressure device (as negative pressure pump), to provide negative pressure.
Embodiment
The IgG antibody of marked by fluorescein isothiocyanate being taken with liquid-transfering gun, sprays sample in reagent 13, spontaneously dries；?
Two passage endoperidium antibody；Take people's whole blood sample to add in sample cell 11, chip is inserted while third channel in detecting instrument
Negative pressure is connect, vacuum cavitations sample flow rate is provided, sample carries out hemocyte filtration by hemocyte drainage screen 18, the sample after filtration
Enter reagent 13 in reacted with fluorescent-labeled antibody after entrance second channel certain with coated antibody response when
Between, after the completion of reaction, instrument automatic detection signal simultaneously provides testing result, and remaining liq enters waste liquid pool 15.Whole process exists
Complete within 15min.
Claims (10)
1. the instant detection means of a kind of POCT, it is characterised in that the detection means includes cover plate and base plate；
The base plate includes first surface, is sequentially provided with sample cell, first groove, reagent, the second ditch on the first surface
Groove, waste liquid pool and the 3rd groove, the sample cell is connected with the reagent by first groove, and the reagent passes through second
Groove is connected with the waste liquid pool, and the waste liquid pool is connected to the border of the first surface by the 3rd groove；
The base plate is fitted tightly with the cover plate by first surface, so that the cover plate and first groove, second groove
And the 3rd form first passage, second channel and third channel between groove respectively, the waste liquid pool by third channel with big
Gas is connected；The cover plate is provided with the adding mouth of insertion in position corresponding with the sample cell；
Hemocyte drainage screen is provided with the first passage；Be provided with least one test point in the second channel, described extremely
A few test point is formed by the antibody molecule being coated on second channel inwall respectively；Include in the reagent with described
The corresponding fluorescent-labeled antibody of antibody molecule.
2. detection means according to claim 1, it is characterised in that the first passage, second channel and third channel
It is separately linear type or Uncrossed shaped form.
3. detection means according to claim 1 and 2, it is characterised in that the hemocyte drainage screen passes through draw-in groove cuttage
In the first passage.
4. the detection means according to any one of claim 1-3, it is characterised in that the test point passes through point sample legal system
For in the bottom of the second channel, the fluorescently-labeled antibody is sprayed on the bottom of the reagent by spraying process.
5. the detection means according to any one of claim 1-4, it is characterised in that the sample cell, first groove, examination
Agent pond, second groove, the bottom of waste liquid pool and the 3rd groove are concordantly arranged.
6. the detection means according to any one of claim 1-5, it is characterised in that the volume of the waste liquid pool is more than sample
Product pond, the volume of the sample cell is more than reagent.
7. the detection means according to any one of claim 1-6, it is characterised in that the first surface is provided with positioning
Post, the cover plate is provided with the location hole for matching with the locating dowel on first surface.
8. the detection means according to any one of claim 1-7, it is characterised in that the first groove, second groove
It is 300-5000 μm with the width of the 3rd groove, depth is 300-5000 μm.
9. the detection means according to any one of claim 1-8, it is characterised in that the material choosing of the cover plate and base plate
Self-polycarbonate, polydimethylsiloxane, polystyrene and lucite.
10. the detection means according to any one of claim 1-9, it is characterised in that the detection means also includes to bear
Crimping mouth, one end of the negative port is connected with the third channel, and the other end extends to the border of the base plate and cover plate
Outward.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610819201.3A CN106442962A (en) | 2016-09-12 | 2016-09-12 | POCT instant detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610819201.3A CN106442962A (en) | 2016-09-12 | 2016-09-12 | POCT instant detection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106442962A true CN106442962A (en) | 2017-02-22 |
Family
ID=58168808
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610819201.3A Pending CN106442962A (en) | 2016-09-12 | 2016-09-12 | POCT instant detection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106442962A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107014989A (en) * | 2017-05-05 | 2017-08-04 | 陈任远 | A kind of Novel biological chip box |
| CN107271440A (en) * | 2017-07-01 | 2017-10-20 | 中山大学 | The portable detector and method of a kind of parasite egg |
| CN108646012A (en) * | 2018-07-20 | 2018-10-12 | 苏州芯海智能科技有限公司 | A kind of paper chip quickly detected |
| CN108704680A (en) * | 2018-05-24 | 2018-10-26 | 深圳市帝迈生物技术有限公司 | A kind of micro-fluidic chip and immunofluorescence analysis instrument |
| CN108896751A (en) * | 2018-05-24 | 2018-11-27 | 深圳市帝迈生物技术有限公司 | A kind of micro-fluidic chip and immunofluorescence analysis instrument |
| CN111999511A (en) * | 2019-05-11 | 2020-11-27 | 南京岚煜生物科技有限公司 | Real-time monitoring method |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107014989A (en) * | 2017-05-05 | 2017-08-04 | 陈任远 | A kind of Novel biological chip box |
| CN107271440A (en) * | 2017-07-01 | 2017-10-20 | 中山大学 | The portable detector and method of a kind of parasite egg |
| CN108704680A (en) * | 2018-05-24 | 2018-10-26 | 深圳市帝迈生物技术有限公司 | A kind of micro-fluidic chip and immunofluorescence analysis instrument |
| CN108896751A (en) * | 2018-05-24 | 2018-11-27 | 深圳市帝迈生物技术有限公司 | A kind of micro-fluidic chip and immunofluorescence analysis instrument |
| CN108704680B (en) * | 2018-05-24 | 2023-04-18 | 深圳市帝迈生物技术有限公司 | Micro-fluidic chip and immunofluorescence analyzer |
| CN108646012A (en) * | 2018-07-20 | 2018-10-12 | 苏州芯海智能科技有限公司 | A kind of paper chip quickly detected |
| CN111999511A (en) * | 2019-05-11 | 2020-11-27 | 南京岚煜生物科技有限公司 | Real-time monitoring method |
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Application publication date: 20170222 |