CN211179850U - A kind of magnetic particle light-emitting double-layer microfluidic chip and detection system - Google Patents

Abstract

The utility model belongs to the technical field of luminescence immune detection of microfluidic chips, in particular to a magnetic particle luminescence double-layer microfluidic chip and a detection system. The chip includes a top plate and a bottom plate, the top plate includes a sample application part, a labeling ligand storage part and a sample mixing area, and the sample mixing area is respectively connected with the sample application part and the labeling ligand storage part; the bottom plate includes a flow guiding area, a magnetic particle coating part , a cleaning area, a detection area and a cleaning liquid storage part, the inside of the guide area is provided with a groove whose height is lower than the bottom wall of the magnetic particle coating part and a guide part arranged on the groove and connected to the magnetic particle coating part. The sample enters from the sample adding part, mixes with the labeled ligand in the sample mixing area, and then enters the groove of the guide area. The sample can only enter the magnetic particle coating part from the guide part, and then mix and react with the magnetic particle ligand, and after cleaning in the cleaning area, the luminescence detection is realized in the detection area.

Description

A kind of magnetic particle light-emitting double-layer microfluidic chip and detection system

technical field

The utility model belongs to the technical field of luminescence immune detection of microfluidic chips, in particular to a magnetic particle luminescence double-layer microfluidic chip and a detection system.

Background technique

At present, there are two main development trends in in vitro diagnosis (IVD): one is automation and integration, that is, using fully automated, highly sensitive large-scale instruments and equipment in the central laboratory supporting large hospitals to achieve high-precision disease analysis and diagnosis ; Another kind of miniaturization and bedside, that is, through a small and simple handheld device, to achieve rapid on-site analysis and diagnosis. However, small hospitals have insufficient funds and small sample sizes, and are not suitable for purchasing expensive large-scale equipment. As a result, the rapid detection equipment used in most hospitals at this stage is mainly test strips and their supporting equipment, but the test strips can only achieve qualitative or semi-quantitative detection, with low detection sensitivity, poor specificity, poor repeatability, and obvious interference. Due to China's large population, aging population, and morbidity rates, relying solely on large hospitals has been overwhelmed. Therefore, it has become extremely urgent to develop rapid detection methods and equipment with simple operation, high sensitivity, good repeatability and accurate quantification.

Microfluidic chip technology integrates basic operation units such as sample preparation, reaction, separation, and detection in biological, chemical, and medical analysis processes into a micron-scale chip to automatically complete the entire analysis process. Due to its huge potential in biology, chemistry, medicine and other fields, it has developed into a multidisciplinary research field such as biology, chemistry, medicine, fluids, materials, machinery, etc., and has been used in biomedical research, biochemical testing, forensic identification, etc. field. However, the existing microfluidic chip is provided with a top plate and a bottom plate. When the sample flows from the top plate to the bottom plate, since the channel of the bottom plate is at the same level, the sample will automatically flow backward along the channel due to the effect of gravity. In some test items, only a small amount of the sample can be used to complete the test and analysis. If the sample flows from the top plate to the bottom plate and flows directly in the bottom plate, it will greatly affect the final test result and cause errors in the test results. In addition, the sample flows along the channel of the bottom plate, the flow path of the sample cannot be limited, and the sample flowing to the detection area cannot be guaranteed to be completely filtered, which further affects the final detection result.

Utility model content

The embodiments of the present utility model provide a magnetic particle luminescent double-layer microfluidic chip and a detection system, which aim to solve the problem that in the existing microfluidic chip, after the sample flows from the top plate to the bottom plate, it directly flows in the bottom plate, and the sample cannot be limited. The problem of flow through the path.

The embodiments of the present invention are implemented in this way, providing a magnetic particle light-emitting double-layer microfluidic chip, the chip includes: a top plate, the top plate includes a sample application part, a labeling ligand storage part and a sample mixing area, the The labeling ligand storage part is provided with labeling ligands, and the sample mixing area is communicated with the sample adding part and the labeling ligand storage part respectively; the bottom plate is arranged on the top plate, and the bottom plate includes The sample mixing area is interconnected with the guide area, the magnetic particle coating section is connected with the flow guide area, the cleaning area is connected with the magnetic particle coating section, and the detection area is connected with the cleaning area. , a cleaning liquid storage part that communicates with the cleaning area, the inside of the guide area is provided with a groove with a height lower than the bottom wall of the magnetic particle coating part, which is arranged on the groove and connected to the magnetic A guide part of the particle coating part, a cut-off groove arranged under the front end of the guide part, and a blocking part arranged on the guide part, and a magnetic particle ligand solution is arranged inside the magnetic particle coat part , the cleaning liquid storage part is provided with cleaning liquid inside.

Further, the top plate further includes an air pump that is communicated with the sample adding portion.

Furthermore, the top plate is provided with elastic members at corresponding positions of the air pump and the sample mixing area.

Furthermore, the inside of the air pump is provided with a porous elastic member.

Further, the sample adding portion includes a sample adding port and a cover for opening or closing the sample adding port, and the sample adding portion further includes a rubber ring provided on the sample adding port.

Further, the top plate and the bottom plate are provided with limit notches at positions corresponding to each other.

Further, the top plate is provided with a first buckle or a first buckle, and the bottom plate is provided with a second buckle or a second buckle, and the first buckle and the second buckle are The top plate and the bottom plate are engaged with each other through the mutual cooperation of the first card slot and the second buckle.

Further, a part or all of the area of at least one side of the bottom plate is provided with a single-sided adhesive substance.

Further, the surface of the top plate or the bottom plate is provided with a product label, and the surface of the top plate or the bottom plate is provided with a two-dimensional code label.

Further, the top plate is provided with a magnetic attraction escape hole on the corresponding communication track with the magnetic particle coating part, the cleaning area and the detection area.

Furthermore, the bottom plate also includes a waste liquid pool interconnected with the cleaning area.

Further, the bottom plate further includes a luminescent liquid storage part which is mutually communicated with the detection area, and a luminescent liquid is arranged inside the luminescent liquid storage part.

Further, the top plate is provided with a cleaning liquid escape hole and a luminescent liquid escape hole at corresponding positions of the cleaning liquid storage part and the luminescent liquid storage part.

Further, a fluorescent liquid is provided inside the labeled ligand storage part.

The utility model also provides a magnetic particle luminescence double-layer microfluidic detection system, the detection system comprises: the magnetic particle luminescence double-layer microfluidic chip as described above; A magnet unit for moving magnetic particles; a squeezing unit for breaking the labeled ligand storage part and the cleaning solution storage part, so that the labeled ligand and the cleaning solution flow out; used for detecting the detection The detection unit of the luminescence signal in the zone.

The beneficial effect of the present utility model is that, compared with the prior art, the present utility model designs a magnetic particle luminescent double-layer microfluidic chip and a detection system, the sample enters from the sample adding part, and the labeled ligand is mixed in the sample mixing area. They are mixed with each other and then enter the groove of the diversion area. Since the groove is lower than the bottom wall of the magnetic particle coating part, the sample in the groove can be sucked away by capillary action. The sample can only enter the magnetic particle coating part from the guide part, and then fully mix and react with the magnetic particle ligand, and after being cleaned by the cleaning liquid in the cleaning area, the luminescence detection is realized in the detection area.

Description of drawings

1 is an exploded schematic diagram of a magnetic particle light-emitting double-layer microfluidic chip provided by an embodiment of the present invention;

Fig. 2 is a partial enlarged view of Fig. 1;

FIG. 3 is another exploded schematic diagram of the magnetic particle light-emitting double-layer microfluidic chip provided by the embodiment of the present invention.

Detailed ways

In order to make the purpose, technical solutions and advantages of the present utility model more clearly understood, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention, and are not intended to limit the present invention.

In the present invention, a magnetic particle luminescent double-layer microfluidic chip and a detection system are designed. The sample enters from the sample adding part 11 , mixes with the labeling ligand in the sample mixing area 13 , and then enters the groove 211 of the guide area 21 . , since the groove 211 is lower than the bottom wall of the magnetic particle coating part 22, the sample in the groove 211 can be sucked away by capillary action, and the sample enters the magnetic particle coating part 22 from the guide part 212, and then mixes with the magnetic particles. The body is fully mixed and reacted, and after the cleaning area 23 is cleaned by the cleaning liquid, the luminescence detection is realized in the detection area 24 .

Example 1

Referring to FIG. 1 and FIG. 2 , the first embodiment provides a magnetic particle light-emitting double-layer microfluidic chip. The magnetic particle light-emitting double-layer microfluidic chip includes a top plate 1 and a bottom plate 2 disposed on the top plate 1 .

The top plate 1 includes a sample application part 11 , a labelled ligand storage part 12 and a sample mixing area 13 . The labelled ligand storage part 12 is provided with a labelled ligand inside, and the sample mixing area 13 is respectively connected with the sample application part 11 and the labelled ligand storage part 12 . interconnected.

The bottom plate 2 includes a guide area 21 that communicates with the sample mixing area 13 , a magnetic particle coating portion 22 that communicates with the guide area 21 , a cleaning area 23 that communicates with the magnetic particle coating portion 22 , and the cleaning area 23 The connected detection area 24, the cleaning liquid storage part 25 connected with the cleaning area 23, the inside of the guide area 21 is provided with a groove 211 whose height is lower than the bottom wall of the magnetic particle coating part 22, and is arranged on the groove 211 and connected The guide part 212 of the magnetic particle coating part 22 , the blocking groove 213 provided under the front end of the guide part 212 , and the blocking part 214 provided on the guide part 212 , the guide part 212 can be selected as a filter Blood film, the blocking part 214 can be selected from plastic paper, the bottom wall of the cutting groove 213 is also provided with a blood filtering film, the height of the cutting groove 213 is lower than the height of the groove 211, and the magnetic particle coating part 22 is provided with magnetic particles inside Ligand solution, the cleaning solution is provided inside the cleaning solution storage part 25 .

Taking the sample as a whole blood sample as an example, the sample to be tested can be put into the sample adding part 11, the sample enters the sample mixing area 13 through the sample adding part 11, and the labeled ligand inside the labeled ligand storage part 12 also enters the In the sample mixing area 13 , the sample and the labeled ligand are mixed in the sample mixing area 13 , and then enter the guide part 212 of the guide area 21 from the sample mixing area 13 . After passing through the guide area 21 , the plasma in the sample is separated from the blood cells, the plasma enters the magnetic particle coating part 22 from the guide part 212 , and the blood cells remain in the guide area 21 . Since the grooves 211 of the guide region 21 are lower than the bottom wall of the magnetic particle coating portion 22 , the height of the guide portion 212 is also lower than the bottom wall of the magnetic particle coating portion 22 . It will automatically enter the magnetic particle coating part 22 from the guide part 212 due to the action of gravity, but the sample will be sucked away from the guide part 212 by capillary action. In this way, it can be sucked away in a larger volume of the sample to meet the detection requirements. A smaller volume of sample is required to avoid the large volume of the sample affecting the test results. Moreover, when the sample passes into the guide area 21, due to the action of the blocking groove 213 and the blocking part 214, the sample can only flow over the blocking part 214, and the sample that does not flow through the guide part 212 will fall into the blocking part 214. In the groove 213 , the sample can only flow into the capillary channel of the magnetic particle coating part 22 from the guiding part 212 , and the flow area of the sample flowing into the magnetic particle coating part 22 is limited, and will not flow out of the guiding part 212 As a result, the detection sample flows into the capillary channel of the magnetic particle coating part 22 from other regions, so that the filtering effect is better and the detection result is more accurate. It is worth mentioning that, if the test sample is whole blood, the red blood cells will not diffuse into the capillary channel of the magnetic particle coating part 22 from the front end of the guide part 212, but will be subjected to the capillary action of the capillary channel to absorb the filtered plasma and enter it. Magnetic particle coating portion 22 .

After the deflected sample reaches the corresponding position of the magnetic particle coating part 22, the analyte in the sample reacts with the magnetic particle ligand solution, the external magnet collects the magnetic particles in the magnetic particle ligand solution, and the reacted The sample enters the cleaning area 23 , and the external magnet also drives the magnetic particles into the cleaning area 23 . At this time, the cleaning liquid in the cleaning liquid storage part 25 is released, and the cleaning liquid enters the cleaning area 23 to clean the magnetic particles in the sample, and then the sample enters the detection area 24 from the cleaning area 23, and the external magnet also drives the magnetic particles into the detection area. Zone 24, thereby realizing quantitative detection of the analyte in the sample.

Wherein, the blood filter membrane is preset in the guide area 21, wherein the blood filter membrane can separate the liquid from the cells through the physical pore diameter or biological/chemical reagents, realize the separation of plasma and red blood cells, the plasma flows to the magnetic particle coating part 22, and the Red blood cells stay on the filter membrane, thereby reducing red blood cell interference with the test results. Among them, biological/chemical reagents include coagulants, etc., which can connect red blood cells, form clots, and increase their size, and the increased size of red blood cells is more likely to be blocked by the mesh structure of the blood filtering membrane, thereby more effectively reducing the impact of red blood cells on experiments. result interference.

The cleaning solution is stored in the cleaning solution storage unit 25 in advance, and the cleaning solution is used to clean the magnetic beads to remove non-specifically adsorbed analytes, luminescent agent labels, and other substances that affect the detection results. The cleaning solution mainly includes buffer reagents, proteins and surfactants, wherein the buffer reagents include but are not limited to borate, phosphate, Tris-HCl and acetate, etc. The pH range of the cleaning solution is 6.0-10.0. The proteins include but are not limited to bovine serum albumin, casein and the like. The surface activity includes, but is not limited to, Tween 20, Tween 80, Triton X-100, polyethylene glycol, polyvinylpyrrolidone, and the like. Preferably, in this embodiment, the cleaning solution used is a pH7.0 Tris-HCl buffer containing bovine serum albumin, Tween 20 and Proclin300.

In this embodiment, the labeling ligand storage part 12 , the magnetic particle coating part 22 and the cleaning liquid storage part 25 are sealed cavities, and the sealing material used is an elastic material or a high-barrier film, specifically glass, plastic, rubber, aluminum foil or High-barrier film, wherein the sealing material can be composed of the same material or a combination of multiple materials. Under physical squeezing, the labeled ligand storage portion 12, the magnetic particle coating portion 22, and the cleaning solution storage portion 25 can be partially ruptured, thereby releasing the stored material.

Embodiment 2

Referring to FIG. 1 , on the basis of the first embodiment, the top plate 1 of the fourth embodiment is also provided with an air pump 14 that communicates with the sample adding portion 11. The air pump 14 is an air bag built into the top plate 1. The air bag is released, so that the air in the air bag repeatedly enters and exits the sample adding part 11 and the sample mixing area 13 , thereby driving the liquid flow in the top plate 1 .

The air pump 14 is used for absorbing or squeezing the air in the sample mixing area 13, so that the sample and the labeled ligand flow to the sample mixing area 13, and by repeatedly absorbing or squeezing the air in the top plate 1, the sample and the labeled ligand are in the sample. The mixing area 13 is fully mixed, and after mixing, the sample is driven from the sample mixing area 13 into the guide area 21 .

It should be noted that since the air pump 14 needs to work in a sealed environment, in order to seal the inside of the chip, it is necessary to close the cover after adding the sample to the sample injection port to seal the inside of the chip and ensure the driving effect of the air pump 14 .

Embodiment 3

Referring to FIG. 1 , on the basis of the second embodiment, the top plate 1 of the third embodiment is provided with elastic members in the air pump 14 and the sample mixing area 13 . The elastic member is optionally glued to one side of the top plate 1 , and the side of the top plate 1 is adjacent to the bottom plate 2 . The elastic member can provide elastic effect for the air pump 14 and the sample mixing area 13 .

Embodiment 4

Referring to FIG. 1 , on the basis of the second embodiment, the air pump 14 of the fourth embodiment is provided with a porous elastic member, and the porous elastic member can be selected as a sponge. The air pump 14 needs to be pressed repeatedly. After the air pump 14 is pressed, the pressing force is removed, and the porous elastic member can provide a rebound force to the pump surface of the air pump 14, so that the pump surface becomes a tight state. On the other hand, the porous elastic member is provided with a plurality of orifices, so that the porous elastic member can reduce the internal volume of the air pump 14 , which is helpful for further miniaturization of the air pump 14 .

Embodiment 5

Referring to FIG. 1 , on the basis of the first embodiment, the sample adding part 11 of the fifth embodiment includes a sample adding port and a cover for opening or closing the sample adding port.

When the cover is open, the sample can be added to the injection port from the outside, and after the sample is added, the cover is closed to close the injection port.

In detail, the cover is provided with a first clamping piece or a first clamping hole, and a second clamping hole or a second clamping piece is provided at a position adjacent to the sample feeding port, and the first clamping piece and the second clamping hole cooperate with each other. , or through the mutual cooperation between the first clamping hole and the second clamping member, so that the cover closes the sample filling port. In addition, the cover is also provided with a sealing member adapted to the sample feeding port. When the sealing cover is closed, the sealing member is inserted into the sample feeding port at the same time to prevent the sample from leaking from the sample feeding port.

In addition, the sample adding part 11 also includes a rubber ring arranged on the sample adding port. Since the sample is often added externally through the pipette tip, the rubber ring has elasticity, which is helpful for sealing with the pipette tip, so as to make the pipette tip more smoothly. Inject the sample from the injection port.

Embodiment 6

On the basis of the first embodiment, the top plate 1 and the bottom plate 2 of the sixth embodiment are provided with limit notches at positions corresponding to each other. The limiting notch is a notch provided at one end of the top plate 1 and the bottom plate 2 , which is not shown in the figure. Since the magnetic particle light-emitting double-layer microfluidic chip is in the detection process, in order to avoid the deviation of the magnetic particle light-emitting double-layer microfluidic chip, it is necessary to perform a limit operation on the magnetic particle light-emitting double-layer microfluidic chip. The limit notch and the external limit structure cooperate with each other, which can firmly fix the chip during the detection process to avoid its deviation, thereby ensuring the smooth progress of the detection.

Embodiment 7

Referring to FIG. 1 , on the basis of the first embodiment, the top plate 1 of the seventh embodiment is provided with a first clip or a first clip, and the bottom plate 2 is provided with a second clip or a second clip. The top plate 1 and the bottom plate 2 are engaged with each other by the mutual cooperation of the buckle and the second buckle, or through the mutual cooperation of the first buckle and the second buckle. Through the above-mentioned snap-fit manner, the top plate 1 and the bottom plate 2 can be detachably arranged, which is beneficial for the operator to perform inspection or replacement. After the fastening, the top plate 1 and the bottom plate 2 can be firmly fixed to each other.

Of course, the top plate 1 and the bottom plate 2 can also be combined with each other in other ways, which will not be repeated here.

Embodiment 8

Referring to FIG. 3 , on the basis of the first embodiment, in the eighth embodiment, a part or all of the area of at least one side of the bottom plate 2 is provided with a single-sided adhesive substance 3, and the single-sided adhesive substance 3 can be selected as a single-sided adhesive substance. adhesive tape. Preferably, both sides of the bottom plate 2 are provided with a single-sided adhesive substance 3 , and the single-sided adhesive substance 3 has a sealing effect on the bottom plate 2 . In this embodiment, the one-sided adhesive substance 3 is provided with a vacant area at the corresponding position of the cleaning liquid storage part.

Embodiment 9

Referring to FIG. 1 , on the basis of the first embodiment, the surface of the top plate 1 of the ninth embodiment is provided with a product label, and the product label is provided with the relevant introduction of the chip, such as the name of the chip, the detection target of the chip, and the like.

And, the surface of the top plate 1 or the bottom plate 2 is provided with a two-dimensional code label, and the camera on the external detection instrument can scan the two-dimensional code on the two-dimensional code label, thereby reading the corresponding data, such as reading the name of the chip, the Detection target, product calibration information, etc. It should be noted that the setting position of the two-dimensional code label depends on the facing position of the camera of the external detection instrument.

Embodiment ten

Referring to FIG. 1 , on the basis of the first embodiment, the top plate 1 of the tenth embodiment is provided with a magnetic attraction escape hole 15 on the corresponding communication track with the magnetic particle coating part 22 , the cleaning area 23 and the detection area 24 . The external magnets move along the set direction of the magnetic attraction abdication hole 15 , and drive the magnetic particles to move along the magnetic particle coating portion 22 , the cleaning area 23 and the detection area 24 in sequence. In addition, the magnetic attraction abdication hole 15 is provided, so that the magnet can be closer to the bottom plate 2, avoiding the space between the bottom plate 2 and the top plate 1, and greatly improving the reliability of the magnetic attraction.

Embodiment 11

Referring to FIG. 1 , on the basis of the first embodiment, the bottom plate 2 of the eleventh embodiment is provided with a waste liquid pool 27 which is interconnected with the cleaning area 23 . The waste liquid pool 27 can collect the waste liquid after cleaning and reaction, and can Reduce the interference of waste liquid on the final detection, and effectively improve the detection accuracy. A plurality of waste liquid pools 27 may be provided.

Embodiment 12

Referring to FIG. 1 , on the basis of Embodiments 1 to 11, the bottom plate 2 of the twelfth embodiment further includes a luminescent liquid storage part 26 that communicates with the detection area 24 , and the luminescent liquid storage part 26 is provided with a luminescent liquid inside. .

After the sample is cleaned in the cleaning area 23, it enters the detection area 24. At this time, the luminescent fluid in the luminescent fluid storage unit 26 is released, and the luminescent fluid reacts with the sample to emit a luminescent signal. The external detection instrument detects the intensity of the luminescent signal.

The luminescent liquid is stored in the luminescent liquid storage part 26 in advance, and the luminescent liquid is used for further cleaning the magnetic beads or enhancing the luminescent signal. The luminescence liquid includes a substrate liquid and a luminescence enhancement liquid. The substrate liquid can be selected from an acidic solution containing luminol or an acidic solution containing adamantane, and the luminescence enhanced liquid can be selected from an alkaline solution containing a benzene derivative.

It is worth mentioning that, considering that the base liquid and the luminescence enhancement liquid should not be mixed and stored for a long time, the luminescence liquid storage part 26 can be provided with a first luminescence liquid storage part and a second luminescence liquid storage part, and the first luminescence liquid storage part is internally stored. There is a substrate liquid, a luminescence enhancement liquid is stored in the second luminescence liquid storage part, and a luminescence liquid mixing area is arranged on the bottom plate 2, and the luminescence liquid mixing area is respectively connected to the detection area 24, the first luminescence liquid storage part and the second luminescence liquid. liquid storage. When the first luminescent liquid storage part and the second luminescent liquid storage part are released, the substrate liquid and the luminescence enhancement liquid enter the luminescent liquid mixing area and mix with each other, and then enter the detection area 24 after mixing evenly.

The luminescent liquid storage part 26 is a sealed cavity, and the sealing material used is an elastic material or a high-barrier film, specifically glass, plastic, rubber, aluminum foil or a high-barrier film, wherein the sealing material can be composed of the same material, or can be a variety of materials combined. Under physical pressing, the luminescent fluid storage part 26 can be partially ruptured, thereby releasing the stored luminescent fluid.

The labeled ligand is pre-stored in the labeled ligand storage unit 12, and when the labeled ligand includes an enzyme-labeled ligand, the enzyme can be selected as one or more of horseradish peroxide and alkaline phosphatase, Ligands can be selected from one or more of antigens, antibodies, haptens and nucleic acids. The magnetic particle ligand solution is pre-stored in the magnetic particle coating part 22. The magnetic particle ligand solution includes magnetic particles, carbohydrates, buffer reagents, proteins, surfactants and preservatives. The magnetic particles include but are not limited to ferric oxide. and ferric oxide compounds.

When the labeled ligand includes an enzyme-labeled ligand, the enzyme binds or competes with the analyte in the sample to form an enzyme-labeled ligand; the magnetic particle label binds or competes with the analyte in the sample to form a magnetic bead-labeled ligand , the two ligands can be the same or different; magnetically labeled ligands, enzyme-labeled ligands include nucleic acids, antigens, monoclonal antibodies, polyclonal antibodies and hormone receptors, analytes in samples include DNA, small molecules (drugs) or drugs), antigens, antibodies, hormones, antibiotics, bacteria or viruses, and other biochemical markers.

In this embodiment, the labeled ligand can be bound to the magnetic particle ligand solution (eg, double-antibody sandwich method), or the labeled ligand can compete with the labeled ligand (eg, a competition method). The enzyme-labeled ligand can be the same as the magnetic particle ligand solution, or it can be different. Preferably, in an embodiment of the present invention, two different antibodies are selected as the labeling ligand and the magnetic particle ligand solution is used to detect the analyte by a double-antibody sandwich method. In another embodiment of the present invention, one antigen and one antibody are selected as the labeling ligand and the magnetic particle ligand solution, respectively, to detect the analyte by a competitive method.

Embodiment thirteen

Referring to FIG. 1 , on the basis of the twelfth embodiment, the top plate 1 of the thirteenth embodiment is provided with a cleaning liquid leaving hole and a luminescent liquid at the corresponding positions of the cleaning liquid storage part 25 and the luminescent liquid storage part 26 on the bottom plate 2 . Make way for holes.

Since the cleaning liquid storage part 25 and the luminescent liquid storage part 26 are usually in a certain shape, such as a cylinder, in order to adapt to the shapes of the cleaning liquid storage part 25 and the luminescent liquid storage part 26 and avoid partial protrusion of the chip, the top plate 1 is provided with The cleaning solution makes way for the hole and the luminescent solution makes way for the hole.

In this embodiment, the removal hole for cleaning liquid and the removal hole for luminescent liquid together form the removal hole 16 .

Embodiment 14

Referring to FIG. 1 , on the basis of Embodiments 1 to 11, the labeled ligand storage part 12 of Embodiment 14 is provided with a fluorescent agent inside. That is to say, the labeled ligand includes a fluorescent agent-labeled ligand, and the fluorescent agent can be selected from one or more of acridine ester, ABEI, fluorescent dye, fluorescent protein and fluorescent microsphere, and the ligand can be selected as One or more of antigens, antibodies, haptens and nucleic acids. Among them, acridine ester, ABEI and luminescent liquid can directly emit light after the interaction; while fluorescent dyes, fluorescent proteins and fluorescent microspheres need excitation light source, but no luminescent liquid.

When the labeled ligand includes a ligand labeled with a fluorescent agent, the fluorescent agent binds or competes with the analyte in the sample to form a fluorescent agent-labeled ligand; the magnetic particle ligand solution binds or competes with the analyte in the sample to form Magnetic beads labeled ligands, the two ligands may be the same or different; magnetic particle ligand solutions, fluorophore-labeled ligands containing nucleic acids, antigens, monoclonal antibodies, polyclonal antibodies and hormone receptors, analytes in samples Includes DNA, small molecules (drugs or drugs), antigens, antibodies, hormones, antibiotics, bacteria or viruses, and other biochemical markers.

In this embodiment, the labeled ligand can bind to the magnetic particle ligand solution (eg, double-antibody sandwich method), or the labeled ligand can compete with the magnetic particle ligand solution (eg, competition method). The fluorescent agent-labeled ligand can be the same as the magnetic particle ligand solution, or it can be different. Preferably, in an embodiment of the present invention, two different antibodies are selected as the labeling ligand and the magnetic particle ligand solution is used to detect the analyte by a double-antibody sandwich method.

Embodiment fifteen

The fifteenth embodiment provides a magnetic particle light-emitting double-layer microfluidic detection system, the detection system includes: the magnetic particle light-emitting double-layer microfluidic chip described in the first to fourteenth embodiment; The magnet unit for moving the magnetic particles in the body solution; the extrusion unit for squeezing the labeling ligand storage part 12 and the cleaning liquid storage part 25 to make the labeling ligand and the cleaning liquid flow out; Sample detection unit.

The magnet unit includes a magnet and a drive part for driving the magnet to move. The drive part can be selected as a linear motor, and the output shaft of the linear motor is fixedly connected to the magnet. After the linear motor is started, its output shaft extends to drive the magnet to move, and the magnet attracts the magnetic particles and drives the magnetic particles to move.

The extrusion unit can be optionally a linear motor. After the linear motor is started, its output shaft extends to squeeze the labeling ligand storage part 12 and the cleaning solution storage part 25, so that the labeling ligand and cleaning solution flow out respectively. Of course, a pressing part can also be fixedly installed on the output shaft of the linear motor. The pressing part matches the shape and size of each storage part. The linear motor can be provided with one or more, and the output shaft of the linear motor drives the pressing part to move. , the pressing part squeezes the labeling ligand storage part 12 and the cleaning solution storage part 25 again, so that the labeling ligand and the cleaning solution flow out respectively.

The detection unit can be selected from a photodiode, a photomultiplier tube or an avalanche photodiode. The sample enters the detection area after mixing and reacting through the above process. The mixed sample will have a luminescent signal. In this way, the detection results for the samples are obtained.

When the magnetic particle light-emitting double-layer microfluidic chip is provided with the air pump 14 , the squeezing unit can also be used to squeeze the air pump 14 so as to drive the flow of the liquid on the top plate 1 .

When the magnetic particle luminescent double-layer microfluidic chip is provided with the luminescent liquid storage part 26, the squeezing unit can also be used to squeeze the luminescent liquid storage part 26, so that the luminescent liquid can flow out.

The above are only preferred embodiments of the present invention, and are not intended to limit the present invention. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present invention shall be included in the present invention. within the scope of protection of the utility model.

Claims (15)

1. A magnetic particle light-emitting double-layer micro-fluidic chip is characterized by comprising:

the top plate comprises an adding part, a labeled ligand storage part and a sample mixing region, wherein a labeled ligand is arranged in the labeled ligand storage part, and the sample mixing region is communicated with the adding part and the labeled ligand storage part respectively;

the bottom plate is arranged on the top plate and comprises a flow guide area communicated with the sample mixing area, a magnetic particle coating part communicated with the flow guide area, a cleaning area communicated with the magnetic particle coating part, a detection area communicated with the cleaning area and a cleaning solution storage part communicated with the cleaning area, a groove lower than the bottom wall of the magnetic particle coating part, a flow guide part arranged on the groove and connected with the magnetic particle coating part, a cutting groove arranged below the front end of the flow guide part and a blocking part arranged on the flow guide part are arranged in the flow guide area, a magnetic particle ligand solution is arranged in the magnetic particle coating part, and a cleaning solution is arranged in the cleaning solution storage part.

2. The magnetic particle light-emitting double-layer microfluidic chip of claim 1, wherein the top plate further comprises an air pump communicated with the sample adding part.

3. The magnetic particle light-emitting double-layer microfluidic chip according to claim 2, wherein the top plate is provided with an elastic member at a position corresponding to the air pump and the sample mixing region.

4. The magnetic particle light-emitting double-layer microfluidic chip according to claim 2, wherein a porous elastic member is disposed inside the air pump.

5. The magnetic particle light-emitting double-layer microfluidic chip according to claim 1, wherein the sample application part comprises a sample application port and a cover for opening or closing the sample application port, and the sample application part further comprises a rubber ring arranged on the sample application port.

6. The magnetic particle light-emitting double-layer microfluidic chip of claim 1, wherein the top plate and the bottom plate are provided with limiting notches at positions corresponding to each other.

7. The magnetic particle light-emitting double-layer microfluidic chip according to claim 1, wherein the top plate is provided with a first buckle or a first buckle, the bottom plate is provided with a second buckle or a second buckle, and the top plate and the bottom plate are buckled with each other through the mutual matching of the first buckle and the second buckle or the mutual matching of the first buckle and the second buckle.

8. The magnetic particle light-emitting double-layer microfluidic chip according to claim 1, wherein a single-sided adhesive substance is provided on a part or all of at least one side of the bottom plate.

9. The magnetic particle light-emitting double-layer microfluidic chip of claim 1, wherein a product label is disposed on the surface of the top plate or the bottom plate, and a two-dimensional code label is disposed on the surface of the top plate or the bottom plate.

10. The magnetic particle light-emitting double-layer microfluidic chip of claim 1, wherein the top plate is provided with magnetic attraction yielding holes on corresponding communication tracks with the magnetic particle coating part, the cleaning area and the detection area.

11. The magnetic particle light-emitting double-layer microfluidic chip of claim 1, wherein the base plate further comprises a waste reservoir in communication with the cleaning region.

12. The magnetic particle light-emitting double-layer microfluidic chip according to any one of claims 1 to 11, wherein the bottom plate further comprises a light-emitting liquid storage portion communicated with the detection region, and a light-emitting liquid is disposed inside the light-emitting liquid storage portion.

13. The magnetic particle light-emitting double-layer microfluidic chip according to claim 12, wherein the top plate is provided with a cleaning solution relief hole and a light-emitting solution relief hole at corresponding positions of the cleaning solution storage part and the light-emitting solution storage part.

14. The magnetic particle light-emitting double-layer microfluidic chip according to any one of claims 1 to 11, wherein a fluorescent liquid is provided inside the labeled ligand storage part.

15. A magnetic particle light emitting dual layer microfluidic detection system, the detection system comprising:

the magnetic particle light-emitting double-layer microfluidic chip of any one of claims 1 to 14;

the magnet unit is used for driving the magnetic particles in the magnetic particle ligand solution to move;

a squeezing unit for squeezing the labeled ligand storage part and the cleaning solution storage part to make the labeled ligand and the cleaning solution flow out;

a detection unit for detecting a luminescence signal in the detection zone.

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