CN105660713A - Actinomycete and algin compounded bactericide and application thereof - Google Patents
Actinomycete and algin compounded bactericide and application thereof Download PDFInfo
- Publication number
- CN105660713A CN105660713A CN201610095333.6A CN201610095333A CN105660713A CN 105660713 A CN105660713 A CN 105660713A CN 201610095333 A CN201610095333 A CN 201610095333A CN 105660713 A CN105660713 A CN 105660713A
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- CN
- China
- Prior art keywords
- seaweeds
- microbial inoculum
- actinomycetes
- suppression ratio
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241001446247 uncultured actinomycete Species 0.000 title abstract description 32
- 235000010443 alginic acid Nutrition 0.000 title abstract description 5
- 229920000615 alginic acid Polymers 0.000 title abstract description 5
- 230000000844 anti-bacterial effect Effects 0.000 title abstract description 4
- 239000003899 bactericide agent Substances 0.000 title abstract description 4
- 201000010099 disease Diseases 0.000 claims abstract description 38
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 38
- 239000000203 mixture Substances 0.000 claims abstract description 32
- 239000002270 dispersing agent Substances 0.000 claims abstract description 5
- 239000003995 emulsifying agent Substances 0.000 claims abstract description 5
- 239000000546 pharmaceutical excipient Substances 0.000 claims abstract description 5
- 239000002904 solvent Substances 0.000 claims abstract description 5
- 239000002562 thickening agent Substances 0.000 claims abstract description 5
- 241001474374 Blennius Species 0.000 claims description 42
- 241000186361 Actinobacteria <class> Species 0.000 claims description 25
- 239000002068 microbial inoculum Substances 0.000 claims description 18
- 239000002131 composite material Substances 0.000 claims description 16
- 239000000126 substance Substances 0.000 claims description 11
- -1 sulfonic acid sodium salt Chemical class 0.000 claims description 8
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 6
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims description 5
- 229920002774 Maltodextrin Polymers 0.000 claims description 5
- 239000005913 Maltodextrin Substances 0.000 claims description 5
- 229930195725 Mannitol Natural products 0.000 claims description 5
- 229940035034 maltodextrin Drugs 0.000 claims description 5
- 239000000594 mannitol Substances 0.000 claims description 5
- 235000010355 mannitol Nutrition 0.000 claims description 5
- YWPVROCHNBYFTP-UHFFFAOYSA-N Rubusoside Natural products C1CC2C3(C)CCCC(C)(C(=O)OC4C(C(O)C(O)C(CO)O4)O)C3CCC2(C2)CC(=C)C21OC1OC(CO)C(O)C(O)C1O YWPVROCHNBYFTP-UHFFFAOYSA-N 0.000 claims description 4
- 230000002528 anti-freeze Effects 0.000 claims description 4
- FNAQSUUGMSOBHW-UHFFFAOYSA-H calcium citrate Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O FNAQSUUGMSOBHW-UHFFFAOYSA-H 0.000 claims description 4
- 239000001354 calcium citrate Substances 0.000 claims description 4
- YWPVROCHNBYFTP-OSHKXICASA-N rubusoside Chemical compound O([C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O YWPVROCHNBYFTP-OSHKXICASA-N 0.000 claims description 4
- 239000001509 sodium citrate Substances 0.000 claims description 4
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 4
- 239000001590 sorbitan monolaureate Substances 0.000 claims description 4
- 235000011067 sorbitan monolaureate Nutrition 0.000 claims description 4
- 235000013337 tricalcium citrate Nutrition 0.000 claims description 4
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 3
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical group CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 claims description 3
- XDOFQFKRPWOURC-UHFFFAOYSA-N 16-methylheptadecanoic acid Chemical compound CC(C)CCCCCCCCCCCCCCC(O)=O XDOFQFKRPWOURC-UHFFFAOYSA-N 0.000 claims description 3
- CYDQOEWLBCCFJZ-UHFFFAOYSA-N 4-(4-fluorophenyl)oxane-4-carboxylic acid Chemical compound C=1C=C(F)C=CC=1C1(C(=O)O)CCOCC1 CYDQOEWLBCCFJZ-UHFFFAOYSA-N 0.000 claims description 3
- JRFXQKZEGILCCO-UHFFFAOYSA-N 5,5-dimethyl-1,3-dioxan-2-one Chemical compound CC1(C)COC(=O)OC1 JRFXQKZEGILCCO-UHFFFAOYSA-N 0.000 claims description 3
- RZXLPPRPEOUENN-UHFFFAOYSA-N Chlorfenson Chemical compound C1=CC(Cl)=CC=C1OS(=O)(=O)C1=CC=C(Cl)C=C1 RZXLPPRPEOUENN-UHFFFAOYSA-N 0.000 claims description 3
- 229920000858 Cyclodextrin Polymers 0.000 claims description 3
- YKRGDOXKVOZESV-WRJNSLSBSA-N Paeoniflorin Chemical compound C([C@]12[C@H]3O[C@]4(O)C[C@](O3)([C@]1(C[C@@H]42)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)C)OC(=O)C1=CC=CC=C1 YKRGDOXKVOZESV-WRJNSLSBSA-N 0.000 claims description 3
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 3
- 244000173166 Pyrus ussuriensis Species 0.000 claims description 3
- 235000011572 Pyrus ussuriensis Nutrition 0.000 claims description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical group O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 229920002472 Starch Polymers 0.000 claims description 3
- UEDUENGHJMELGK-HYDKPPNVSA-N Stevioside Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O UEDUENGHJMELGK-HYDKPPNVSA-N 0.000 claims description 3
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 3
- 125000000217 alkyl group Chemical group 0.000 claims description 3
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 3
- 229960003237 betaine Drugs 0.000 claims description 3
- 229920001400 block copolymer Polymers 0.000 claims description 3
- 125000002057 carboxymethyl group Chemical group [H]OC(=O)C([H])([H])[*] 0.000 claims description 3
- 235000014113 dietary fatty acids Nutrition 0.000 claims description 3
- 239000000194 fatty acid Substances 0.000 claims description 3
- 229930195729 fatty acid Natural products 0.000 claims description 3
- 150000004665 fatty acids Chemical class 0.000 claims description 3
- 239000003292 glue Substances 0.000 claims description 3
- KWIUHFFTVRNATP-UHFFFAOYSA-N glycine betaine Chemical group C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 claims description 3
- 229920005610 lignin Polymers 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- YKRGDOXKVOZESV-UHFFFAOYSA-N paeoniflorin Natural products O1C(C)(C2(CC34)OC5C(C(O)C(O)C(CO)O5)O)CC3(O)OC1C24COC(=O)C1=CC=CC=C1 YKRGDOXKVOZESV-UHFFFAOYSA-N 0.000 claims description 3
- 239000001814 pectin Substances 0.000 claims description 3
- 235000010987 pectin Nutrition 0.000 claims description 3
- 229920001277 pectin Polymers 0.000 claims description 3
- 229920002401 polyacrylamide Polymers 0.000 claims description 3
- 229940051841 polyoxyethylene ether Drugs 0.000 claims description 3
- 229920000056 polyoxyethylene ether Polymers 0.000 claims description 3
- 229920001451 polypropylene glycol Polymers 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 239000001397 quillaja saponaria molina bark Substances 0.000 claims description 3
- 229930182490 saponin Natural products 0.000 claims description 3
- 150000007949 saponins Chemical class 0.000 claims description 3
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 claims description 3
- 239000001540 sodium lactate Substances 0.000 claims description 3
- 229940005581 sodium lactate Drugs 0.000 claims description 3
- 235000011088 sodium lactate Nutrition 0.000 claims description 3
- 229940080313 sodium starch Drugs 0.000 claims description 3
- JHJUUEHSAZXEEO-UHFFFAOYSA-M sodium;4-dodecylbenzenesulfonate Chemical compound [Na+].CCCCCCCCCCCCC1=CC=C(S([O-])(=O)=O)C=C1 JHJUUEHSAZXEEO-UHFFFAOYSA-M 0.000 claims description 3
- 239000008347 soybean phospholipid Substances 0.000 claims description 3
- 229940032147 starch Drugs 0.000 claims description 3
- 239000008107 starch Substances 0.000 claims description 3
- 235000019698 starch Nutrition 0.000 claims description 3
- 229940013618 stevioside Drugs 0.000 claims description 3
- OHHNJQXIOPOJSC-UHFFFAOYSA-N stevioside Natural products CC1(CCCC2(C)C3(C)CCC4(CC3(CCC12C)CC4=C)OC5OC(CO)C(O)C(O)C5OC6OC(CO)C(O)C(O)C6O)C(=O)OC7OC(CO)C(O)C(O)C7O OHHNJQXIOPOJSC-UHFFFAOYSA-N 0.000 claims description 3
- 235000019202 steviosides Nutrition 0.000 claims description 3
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 claims description 2
- 229920002307 Dextran Polymers 0.000 claims description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 2
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 2
- 239000002202 Polyethylene glycol Substances 0.000 claims description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 2
- 244000269722 Thea sinensis Species 0.000 claims description 2
- OOCMUZJPDXYRFD-UHFFFAOYSA-L calcium;2-dodecylbenzenesulfonate Chemical compound [Ca+2].CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O.CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O OOCMUZJPDXYRFD-UHFFFAOYSA-L 0.000 claims description 2
- SFNALCNOMXIBKG-UHFFFAOYSA-N ethylene glycol monododecyl ether Chemical compound CCCCCCCCCCCCOCCO SFNALCNOMXIBKG-UHFFFAOYSA-N 0.000 claims description 2
- 239000008103 glucose Substances 0.000 claims description 2
- FZWBNHMXJMCXLU-BLAUPYHCSA-N isomaltotriose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O)O1 FZWBNHMXJMCXLU-BLAUPYHCSA-N 0.000 claims description 2
- 239000008101 lactose Substances 0.000 claims description 2
- 229920001223 polyethylene glycol Polymers 0.000 claims description 2
- 229950008882 polysorbate Drugs 0.000 claims description 2
- 229920000136 polysorbate Polymers 0.000 claims description 2
- 239000001117 sulphuric acid Substances 0.000 claims description 2
- 235000011149 sulphuric acid Nutrition 0.000 claims description 2
- 241000918585 Pythium aphanidermatum Species 0.000 abstract description 20
- 230000000694 effects Effects 0.000 abstract description 11
- 239000000575 pesticide Substances 0.000 abstract description 5
- 230000002265 prevention Effects 0.000 abstract description 3
- 239000007798 antifreeze agent Substances 0.000 abstract 1
- 244000052616 bacterial pathogen Species 0.000 abstract 1
- 238000013329 compounding Methods 0.000 abstract 1
- 230000002195 synergetic effect Effects 0.000 abstract 1
- 238000004383 yellowing Methods 0.000 abstract 1
- 230000001629 suppression Effects 0.000 description 84
- 238000002360 preparation method Methods 0.000 description 69
- 241000196324 Embryophyta Species 0.000 description 24
- 239000003814 drug Substances 0.000 description 21
- 238000005259 measurement Methods 0.000 description 14
- 239000002689 soil Substances 0.000 description 10
- 235000002566 Capsicum Nutrition 0.000 description 9
- 239000006002 Pepper Substances 0.000 description 9
- 241000722363 Piper Species 0.000 description 9
- 235000016761 Piper aduncum Nutrition 0.000 description 9
- 235000017804 Piper guineense Nutrition 0.000 description 9
- 235000008184 Piper nigrum Nutrition 0.000 description 9
- 241000589771 Ralstonia solanacearum Species 0.000 description 8
- 239000003337 fertilizer Substances 0.000 description 8
- 238000000034 method Methods 0.000 description 8
- 230000008485 antagonism Effects 0.000 description 7
- 230000006961 mixed inhibition Effects 0.000 description 7
- 239000008177 pharmaceutical agent Substances 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 5
- 239000000284 extract Substances 0.000 description 5
- 241000091577 Mexicana Species 0.000 description 4
- 241000085543 Acourtia wrightii Species 0.000 description 3
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- 235000016623 Fragaria vesca Nutrition 0.000 description 3
- 240000009088 Fragaria x ananassa Species 0.000 description 3
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 208000010801 foot rot Diseases 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 241000195474 Sargassum Species 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000009847 Cucumis melo var cantalupensis Nutrition 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 102000013275 Somatomedins Human genes 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 239000000783 alginic acid Substances 0.000 description 1
- 229960001126 alginic acid Drugs 0.000 description 1
- 150000004781 alginic acids Chemical class 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 238000005056 compaction Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 239000004062 cytokinin Substances 0.000 description 1
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000032050 esterification Effects 0.000 description 1
- 238000005886 esterification reaction Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 150000004804 polysaccharides Chemical class 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 235000010408 potassium alginate Nutrition 0.000 description 1
- 239000000737 potassium alginate Substances 0.000 description 1
- MZYRDLHIWXQJCQ-YZOKENDUSA-L potassium alginate Chemical compound [K+].[K+].O1[C@@H](C([O-])=O)[C@@H](OC)[C@H](O)[C@H](O)[C@@H]1O[C@@H]1[C@@H](C([O-])=O)O[C@@H](O)[C@@H](O)[C@H]1O MZYRDLHIWXQJCQ-YZOKENDUSA-L 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 235000010413 sodium alginate Nutrition 0.000 description 1
- 239000000661 sodium alginate Substances 0.000 description 1
- 229940005550 sodium alginate Drugs 0.000 description 1
- 239000004016 soil organic matter Substances 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/03—Algae
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Mycology (AREA)
- Pest Control & Pesticides (AREA)
- Virology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Fertilizers (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses an actinomycete and algin compounded bactericide and application thereof. The bactericide is composed of actinomycete, algin and a pesticide carrier, wherein the pesticide carrier is selected from an emulgator, an excipient, a thickener, a solubilizer, a dispersant and an antifreeze agent. By compounding actinomycete and algin, the composition has a good prevention and treatment effect on pathogenic bacteria of soil-borne diseases, has an obvious synergistic effect and can be used for preventing and treating common agricultural diseases such as crop seedling death, root decay, green wilting, blight, withering and yellowing, root rot, stem base rot and damping off.
Description
Technical field
The present invention relates to a kind of bactericide, the microbial inoculum composite particularly to a kind of actinomycetes and seaweeds and application thereof.
Background technology
Actinomycetes are a group gram-positive bacteriums. Actinomycetes because of bacterium colony be unwrapping wire shape and gain the name. It is a prokaryote monoid, is distributed very wide in nature, mainly with sporogenesis, next to that fracture reproduction. Actinomycetes have many application in agricultural, as patent 201010158018.6 discloses a kind of corrosion promoting agent for straw, for stalk fermentation; Patent 201210271924.6 discloses a kind of High-efficiency agricultural microecological compound bacterium agent, and actinomycetes are mixed with other agricultural bacterium can make bio-feritlizer; Patent 201410856464.2 discloses a kind of microbial bacterial agent processing spoil, and useless spoil can be converted into fertilizer by actinomycetes. Actinomycetes are widely used in agricultural, are a kind of agriculturally useful strains.
Seaweeds extracts from sea-plant Sargassum. Seaweeds contains various plants somatomedin and mineral element, chelated metal ions and marine biomaterial, such as cytokinin, Sargassum polysaccharides, alginic acid, sodium alginate, potassium alginate, plant cell can be promoted quickly to divide, plant soon long, strengthen metabolism, improve resistance, promote that pregnant flower bud blooms. But the active substance in seaweeds easily inactivates, it is unfavorable for long-term preservation.
In the cultivation of crop, various soil-borne diseases such as dead Seedling, rotten, blue or green withered, vertical withered, withered and yellow wither, root-rot, brown foot rot, damping off etc. and usually to perplex farmer, solution is usually the special pesticide using various specific aim diseases, the excessive use of pesticide, not only makes soil compaction, and polluted underground water resource, cause the unsustainable development of agricultural, and disease pathogen Drug resistance strengthens, what have to increasing pesticide makes consumption, causes vicious cycle. Traditional Agricultural microbial inoculum and the composite preventing and treating agricultural soil-borne disease of seaweeds can be utilized, still there is no relevant report at present.
Summary of the invention
It is an object of the invention to provide a kind of actinomycetes and the composite microbial inoculum of seaweeds. This microbial inoculum for dead Seedling, rotten, blue or green withered, vertical withered, withered and yellow wither, root-rot, brown foot rot, dampinging off etc. has good prevention effect.
A kind of actinomycetes and the composite microbial inoculum of seaweeds, described microbial inoculum is by actinomycetes, seaweeds and farm chemical carrier composition.
Described farm chemical carrier is emulsifying agent, excipient, thickening agent, solubilizing agent, dispersant, one or more in antifreeze.
Described emulsifying agent is soybean phospholipid, sorbitanmonolaureate, the smooth isostearate of Pyrusussuriensis, polysorbate, laureth, alkyl polyoxyethylene ether sulfonate, one or more in calcium dodecyl benzene sulfonate.
Described excipient is kieselguhr, maltodextrin, cyclodextrin, carboxymethyl starch, sodium starch glycollate, lactose, mannitol, dextran, one or more in glucose.
Described thickening agent is Polyethylene Glycol, lignin sulfonic acid sodium salt, dodecylbenzene sodium sulfonate, apple pulp, is esterified liquid pectin, one or more in calcium citrate.
Described solubilizing agent is polyox-yethylene-polyoxypropylene block copolymer, N-methyl-N-C8-C14-acyl glucamides, paeoniflorin, rubusoside, one or more in stevioside.
Described dispersant is trimerized fatty acids, polyoxy alkylidene carbonyl chain, 5,5-dimethyltrimethylene carbonate methyl ester, SYN glue, sulphuric acid, polyoxyethylene alkylphenol ether, one or more in polyacrylamide.
Described antifreeze is glycine betaine, mannitol, tea saponin, trehalose, sodium lactate, sodium citrate, one or more in Rhizoma amorphophalli powder.
Above-mentioned actinomycetes and the application in anti-plant disease of the seaweeds composite microbial inoculum.
Compared with prior art, there is advantages that the compositions of the present invention adopts actinomycetes and seaweeds composite, the pathogen of soil-borne disease had good prevention effect, have obvious cooperative synergism effect, it is possible to the preventing and treating dead Seedling of crop, rotten, blue or green withered, vertical withered, withered and yellow wither, root-rot, brown foot rot, the agricultural Common Diseases such as damping off.
Detailed description of the invention
Below the specific embodiment of the present invention is described in detail, it is to be understood that protection scope of the present invention is not by the restriction of detailed description of the invention.
The reagent that following example adopt is:
Actinomycetes (Actinomycete) preparation, containing actinomycetes 1X108Individual/g preparation.
Seaweeds, the color Chemical Co., Ltd. in Jinan seven produces.
Use content:
(1) Actinomycete preparation 0.5Kg/ mu;
(2) Actinomycete preparation 1.5Kg/ mu;
(3) seaweeds 0.5Kg/ mu;
(4) seaweeds 1.5Kg/ mu;
(5) Actinomycete preparation 0.5Kg/ mu+seaweeds 0.5Kg/ mu;
(6) Actinomycete preparation 0.5Kg/ mu+seaweeds 1.5Kg/ mu;
(7) Actinomycete preparation 1.5Kg/ mu+seaweeds 0.5Kg/ mu;
(8) Actinomycete preparation 1.5Kg/ mu+seaweeds 1.5Kg/ mu;
(9)CK
Being Wuzhishan Area, Haikou City, Hainan Province for examination soil, soil moisture content (water: dry ground) is 29.90%, pH6.97, soil organic matter content 36.6g/kg, NH4+-N3.6mg/kg, NO3--N56.7mg/kg, available phosphorus 534.0mg/kg, available potassium 1029.0mg/kg, belong to sandy loam.
Embodiment 1 resists dead seedling diseases to test
Choosing the experimental plot that the dead seedling diseases of Fructus Fragariae Ananssae often occurs, before strawberry cultivating, ploughed deeply in soil, uniformly apply the fertilizer of above-mentioned content, comparison (CK) is blank, and Fructus Fragariae Ananssae length, to 5-8 sheet leaf, calculates the dead seedling diseases incidence rate of Fructus Fragariae Ananssae;
Fructus Fragariae Ananssae dead seedling diseases suppression ratio %:100X (check plot Fructus Fragariae Ananssae dead seedling diseases diseased plant number-treatment region Fructus Fragariae Ananssae dead seedling diseases diseased plant number) the dead seedling diseases diseased plant number of/check plot Fructus Fragariae Ananssae;
Calculate suppression ratio according to Colby method (1966) formula and the suppression ratio with actual measurement compares, simply and effectively evaluate the synergy effect of mixture. Computing formula is:
E=X1×X2…×Xn/100(n-1)
E is the theoretical suppression ratio of mixture; N is the quantity of mixed medicament; X1Represent the suppression ratio after using the 1st kind of medicament; X2Represent the suppression ratio after using the 2nd kind of pharmaceutical agent; XnRepresent the suppression ratio after using n medicament. When the actual suppression ratio of mixture is more than theoretical suppression ratio, represent potentiation;When the actual suppression ratio of mixture is less than theoretical suppression ratio, represent antagonism. Measurement result is as shown in table 1:
The mixed inhibition to the dead seedling diseases of Fructus Fragariae Ananssae of table 1 Actinomycete preparation+seaweeds
As it can be seen from table 1 Actinomycete preparation suppression to Fructus Fragariae Ananssae dead seedling diseases mixed with the four of seaweeds kinds of different amounts all shows potentiation, synergism is obvious. Above-mentioned Actinomycete preparation comprises following farm chemical carrier: sorbitanmonolaureate 0.01g/g preparation; Maltodextrin 0.38g/g preparation; Calcium citrate 0.18g/g preparation; Rubusoside 0.03g/g preparation; Sodium citrate 0.05g/g preparation.
The anti-damping-off experiment of embodiment 2
Choosing the experimental plot that Fructus Solani melongenae damping-off often occurs, before Fructus Solani melongenae is planted, ploughed deeply in soil, uniformly apply the fertilizer of above-mentioned content, comparison (CK) is blank, and Fructus Solani melongenae length, to 6-9 sheet leaf, calculates Fructus Solani melongenae damping-off incidence rate;
Fructus Solani melongenae damping-off suppression ratio %:100X (check plot Fructus Solani melongenae damping-off diseased plant number-treatment region Fructus Solani melongenae damping-off diseased plant number)/check plot Fructus Solani melongenae damping-off diseased plant number;
Calculate suppression ratio according to Colby method (1966) formula and the suppression ratio with actual measurement compares, simply and effectively evaluate the synergy effect of mixture. Computing formula is:
E=X1×X2…×Xn/100(n-1)
E is the theoretical suppression ratio of mixture; N is the quantity of mixed medicament; X1Represent the suppression ratio after using the 1st kind of medicament; X2Represent the suppression ratio after using the 2nd kind of pharmaceutical agent; XnRepresent the suppression ratio after using n medicament. When the actual suppression ratio of mixture is more than theoretical suppression ratio, represent potentiation; When the actual suppression ratio of mixture is less than theoretical suppression ratio, represent antagonism. Measurement result is as shown in table 2:
The mixed inhibition to Fructus Solani melongenae damping-off of table 2 Actinomycete preparation+seaweeds
From table 2 it can be seen that Actinomycete preparation suppression to Fructus Solani melongenae damping-off mixed with the four of seaweeds kinds of different amounts all shows potentiation, synergism is obvious. Above-mentioned Actinomycete preparation comprises following farm chemical carrier: the smooth isostearate 0.05g/g preparation of Pyrusussuriensis; Cyclodextrin 0.25g/g preparation; Lignin sulfonic acid sodium salt 0.15g/g preparation; Apple pulp 0.15g/g preparation; N-methyl-N-C8-C14-acyl glucamides 0.01g/g preparation; 5,5-dimethyltrimethylene carbonate methyl ester 0.01g/g preparation; Glycine betaine 0.01g/g preparation.
Embodiment 3 resisting damping-off is tested
Choosing the experimental plot that Fructus Melo damping off often occurs, before Muskmelon Planting, ploughed deeply in soil, uniformly apply the fertilizer of above-mentioned content, comparison (CK) is blank, and Fructus Melo length, to 6-9 sheet leaf, calculates Fructus Melo damping off incidence rate;
Fructus Melo damping off suppression ratio %:100X (check plot Fructus Melo damping off diseased plant number-treatment region Fructus Melo damping off diseased plant number)/check plot Fructus Melo damping off diseased plant number;
Calculate suppression ratio according to Colby method (1966) formula and the suppression ratio with actual measurement compares, simply and effectively evaluate the synergy effect of mixture. Computing formula is:
E=X1×X2…×Xn/100(n-1)
E is the theoretical suppression ratio of mixture; N is the quantity of mixed medicament; X1Represent the suppression ratio after using the 1st kind of medicament; X2Represent the suppression ratio after using the 2nd kind of pharmaceutical agent; XnRepresent the suppression ratio after using n medicament. When the actual suppression ratio of mixture is more than theoretical suppression ratio, represent potentiation; When the actual suppression ratio of mixture is less than theoretical suppression ratio, represent antagonism. Measurement result is as shown in table 3:
The mixed inhibition to Fructus Melo damping off of table 3 Actinomycete preparation+seaweeds
From table 3 it can be seen that Actinomycete preparation suppression to Fructus Melo damping off mixed with the four of seaweeds kinds of different amounts all shows potentiation, synergism is obvious.Above-mentioned Actinomycete preparation comprises following farm chemical carrier: alkyl polyoxyethylene ether sulfonate 0.02g/g preparation; Kieselguhr 0.28g/g preparation; Dodecylbenzene sodium sulfonate 0.05g/g preparation; Polyox-yethylene-polyoxypropylene block copolymer 0.15g/g preparation; SYN glue 0.05g/g preparation; Rhizoma amorphophalli powder 0.05g/g preparation; Sodium lactate 0.01g/g preparation.
The anti-stem rot experiment of embodiment 4
Choosing the experimental plot that Fructus Lycopersici esculenti stem rot often occurs, before tomato planting, ploughed deeply in soil, uniformly apply the fertilizer of above-mentioned content, comparison (CK) is blank, and Fructus Lycopersici esculenti length, to 6-9 sheet leaf, calculates Fructus Lycopersici esculenti stem rot incidence rate;
Fructus Lycopersici esculenti stem rot suppression ratio %:100X (check plot Fructus Lycopersici esculenti stem rot diseased plant number-treatment region Fructus Lycopersici esculenti stem rot diseased plant number)/check plot Fructus Lycopersici esculenti stem rot diseased plant number;
Calculate suppression ratio according to Colby method (1966) formula and the suppression ratio with actual measurement compares, simply and effectively evaluate the synergy effect of mixture. Computing formula is:
E=X1×X2…×Xn/100(n-1)
E is the theoretical suppression ratio of mixture; N is the quantity of mixed medicament; X1Represent the suppression ratio after using the 1st kind of medicament; X2Represent the suppression ratio after using the 2nd kind of pharmaceutical agent; XnRepresent the suppression ratio after using n medicament. When the actual suppression ratio of mixture is more than theoretical suppression ratio, represent potentiation; When the actual suppression ratio of mixture is less than theoretical suppression ratio, represent antagonism. Measurement result is as shown in table 4:
The mixed inhibition to Fructus Lycopersici esculenti stem rot of table 4 Actinomycete preparation+seaweeds
From table 4, it can be seen that Actinomycete preparation suppression to Fructus Lycopersici esculenti stem rot mixed with the four of seaweeds kinds of different amounts all shows potentiation, synergism is obvious. Above-mentioned Actinomycete preparation comprises following farm chemical carrier: soybean phospholipid 0.03g/g preparation; Carboxymethyl starch 0.24g/g preparation; Esterification liquid pectin 0.08g/g preparation; Paeoniflorin 0.05g/g preparation; Trimerized fatty acids 0.05g/g preparation; Tea saponin 0.15g/g preparation; Polyoxyethylene alkylphenol ether 0.01g/g preparation.
Embodiment 5 resistance to bacterial wilt is tested
Choosing the experimental plot that pepper ralstonia solanacearum often occurs, before pepper planting, ploughed deeply in soil, uniformly apply the fertilizer of above-mentioned content, comparison (CK) is blank, and Fructus Capsici length, to 6-9 sheet leaf, calculates pepper ralstonia solanacearum incidence rate;
Pepper ralstonia solanacearum suppression ratio %:100X (check plot pepper ralstonia solanacearum diseased plant number-treatment region pepper ralstonia solanacearum diseased plant number)/check plot pepper ralstonia solanacearum diseased plant number;
Calculate suppression ratio according to Colby method (1966) formula and the suppression ratio with actual measurement compares, simply and effectively evaluate the synergy effect of mixture. Computing formula is:
E=X1×X2…×Xn/100(n-1)
E is the theoretical suppression ratio of mixture; N is the quantity of mixed medicament; X1Represent the suppression ratio after using the 1st kind of medicament; X2Represent the suppression ratio after using the 2nd kind of pharmaceutical agent; XnRepresent the suppression ratio after using n medicament. When the actual suppression ratio of mixture is more than theoretical suppression ratio, represent potentiation; When the actual suppression ratio of mixture is less than theoretical suppression ratio, represent antagonism. Measurement result is as shown in table 5:
The mixed inhibition to pepper ralstonia solanacearum of table 5 Actinomycete preparation+seaweeds
From table 4, it can be seen that Actinomycete preparation suppression to pepper ralstonia solanacearum mixed with the four of seaweeds kinds of different amounts all shows potentiation, synergism is obvious. Above-mentioned Actinomycete preparation comprises following farm chemical carrier: sodium starch glycollate 0.03g/g preparation; Mannitol 0.03g/g preparation; Ethylene glycol 0.24g/g preparation;Hook glycoside 0.05g/g preparation; Stevioside's 0.05g/g preparation; Polyacrylamide 0.05g/g preparation; Trehalose 0.09g/g preparation.
Embodiment 6 resists dead seedling diseases to test
Choosing the experimental plot that the dead seedling diseases of Fructus Fragariae Ananssae often occurs, before strawberry cultivating, ploughed deeply in soil, uniformly apply the fertilizer of above-mentioned content, comparison (CK) is blank, and Fructus Fragariae Ananssae length, to 5-8 sheet leaf, calculates the dead seedling diseases incidence rate of Fructus Fragariae Ananssae;
Fructus Fragariae Ananssae dead seedling diseases suppression ratio %:100X (check plot Fructus Fragariae Ananssae dead seedling diseases diseased plant number-treatment region Fructus Fragariae Ananssae dead seedling diseases diseased plant number) the dead seedling diseases diseased plant number of/check plot Fructus Fragariae Ananssae;
Calculate suppression ratio according to Colby method (1966) formula and the suppression ratio with actual measurement compares, simply and effectively evaluate the synergy effect of mixture. Computing formula is:
E=X1×X2…×Xn/100(n-1)
E is the theoretical suppression ratio of mixture; N is the quantity of mixed medicament; X1Represent the suppression ratio after using the 1st kind of medicament; X2Represent the suppression ratio after using the 2nd kind of pharmaceutical agent; XnRepresent the suppression ratio after using n medicament. When the actual suppression ratio of mixture is more than theoretical suppression ratio, represent potentiation; When the actual suppression ratio of mixture is less than theoretical suppression ratio, represent antagonism. Measurement result is as shown in table 6:
The mixed inhibition to the dead seedling diseases of Fructus Fragariae Ananssae of table 6 Actinomycete preparation+seaweeds
As can be seen from Table 6, Actinomycete preparation suppression to Fructus Fragariae Ananssae dead seedling diseases mixed with the four of seaweeds kinds of different amounts all shows potentiation, and synergism is obvious. Above-mentioned Actinomycete preparation comprises following farm chemical carrier: sorbitanmonolaureate 0.01g/g preparation; Maltodextrin 0.38g/g preparation; Calcium citrate 0.18g/g preparation; Rubusoside 0.03g/g preparation; Sodium citrate 0.05g/g preparation; Mexicana extract 0.02g/g preparation. Above-mentioned mexicana extract, can increase further to the dead seedling diseases inhibitory action of Fructus Fragariae Ananssae, and described mexicana extract is adopted and prepared with the following method: takes mexicana stem and leaf and dries, clay into power, 70% alcoholic solution reflux, extract, of 3-5 times of weight of addition 3 times, merging filtrate, it is evaporated and makes.
Embodiment 7 resists dead seedling diseases to test
Choosing the experimental plot that the dead seedling diseases of Fructus Fragariae Ananssae often occurs, before strawberry cultivating, ploughed deeply in soil, uniformly apply the fertilizer of above-mentioned content, comparison (CK) is blank, and Fructus Fragariae Ananssae length, to 5-8 sheet leaf, calculates the dead seedling diseases incidence rate of Fructus Fragariae Ananssae;
Fructus Fragariae Ananssae dead seedling diseases suppression ratio %:100X (check plot Fructus Fragariae Ananssae dead seedling diseases diseased plant number-treatment region Fructus Fragariae Ananssae dead seedling diseases diseased plant number) the dead seedling diseases diseased plant number of/check plot Fructus Fragariae Ananssae;
Calculate suppression ratio according to Colby method (1966) formula and the suppression ratio with actual measurement compares, simply and effectively evaluate the synergy effect of mixture. Computing formula is:
E=X1×X2…×Xn/100(n-1)
E is the theoretical suppression ratio of mixture; N is the quantity of mixed medicament; X1Represent the suppression ratio after using the 1st kind of medicament; X2Represent the suppression ratio after using the 2nd kind of pharmaceutical agent; XnRepresent the suppression ratio after using n medicament. When the actual suppression ratio of mixture is more than theoretical suppression ratio, represent potentiation; When the actual suppression ratio of mixture is less than theoretical suppression ratio, represent antagonism. Measurement result is as shown in table 7:
The mixed inhibition to the dead seedling diseases of Fructus Fragariae Ananssae of table 7 Actinomycete preparation+seaweeds
As can be seen from Table 7, Actinomycete preparation suppression to Fructus Fragariae Ananssae dead seedling diseases mixed with the four of seaweeds kinds of different amounts all shows potentiation, and synergism is inconspicuous. Above-mentioned Actinomycete preparation comprises following farm chemical carrier: maltodextrin 0.38g/g preparation.
The several specific embodiments being only the present invention disclosed above, but, the present invention is not limited to this, and the changes that any person skilled in the art can think of all should fall into protection scope of the present invention.
Claims (9)
1. actinomycetes and the composite microbial inoculum of seaweeds, it is characterised in that described microbial inoculum is by actinomycetes, seaweeds and farm chemical carrier composition.
2. actinomycetes according to claim 1 and the composite microbial inoculum of seaweeds, it is characterised in that described farm chemical carrier is emulsifying agent, excipient, thickening agent, solubilizing agent, dispersant, one or more in antifreeze.
3. actinomycetes according to claim 2 and the composite microbial inoculum of seaweeds, it is characterized in that, described emulsifying agent is soybean phospholipid, sorbitanmonolaureate, the smooth isostearate of Pyrusussuriensis, polysorbate, laureth, alkyl polyoxyethylene ether sulfonate, one or more in calcium dodecyl benzene sulfonate.
4. actinomycetes according to claim 2 and the composite microbial inoculum of seaweeds, it is characterised in that described excipient is kieselguhr, maltodextrin, cyclodextrin, carboxymethyl starch, sodium starch glycollate, lactose, mannitol, dextran, one or more in glucose.
5. actinomycetes according to claim 2 and the composite microbial inoculum of seaweeds, it is characterised in that described thickening agent is Polyethylene Glycol, lignin sulfonic acid sodium salt, dodecylbenzene sodium sulfonate, apple pulp, is esterified liquid pectin, one or more in calcium citrate.
6. actinomycetes according to claim 2 and the composite microbial inoculum of seaweeds, it is characterised in that described solubilizing agent is polyox-yethylene-polyoxypropylene block copolymer, N-methyl-N-C8-C14-acyl glucamides, paeoniflorin, rubusoside, one or more in stevioside.
7. actinomycetes according to claim 2 and the composite microbial inoculum of seaweeds, it is characterized in that, described dispersant is trimerized fatty acids, polyoxy alkylidene carbonyl chain, 5,5-dimethyltrimethylene carbonate methyl ester, SYN glue, sulphuric acid, polyoxyethylene alkylphenol ether, one or more in polyacrylamide.
8. actinomycetes according to claim 2 and the composite microbial inoculum of seaweeds, it is characterised in that described antifreeze is glycine betaine, mannitol, tea saponin, trehalose, sodium lactate, sodium citrate, one or more in Rhizoma amorphophalli powder.
9. actinomycetes described in claim 1 and the application in anti-plant disease of the seaweeds composite microbial inoculum.
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