CN105410257A - Processing method of green tea and wolfberry fruit polysaccharide beverage - Google Patents
Processing method of green tea and wolfberry fruit polysaccharide beverage Download PDFInfo
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- CN105410257A CN105410257A CN201510875274.XA CN201510875274A CN105410257A CN 105410257 A CN105410257 A CN 105410257A CN 201510875274 A CN201510875274 A CN 201510875274A CN 105410257 A CN105410257 A CN 105410257A
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- green tea
- polysaccharide
- tea
- lbp
- beverage
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- 244000269722 Thea sinensis Species 0.000 title claims abstract description 38
- 150000004676 glycans Chemical class 0.000 title claims abstract description 34
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 34
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 34
- 235000009569 green tea Nutrition 0.000 title claims abstract description 21
- 235000013361 beverage Nutrition 0.000 title claims abstract description 16
- 238000003672 processing method Methods 0.000 title claims abstract description 6
- 244000241838 Lycium barbarum Species 0.000 title abstract description 13
- 235000015459 Lycium barbarum Nutrition 0.000 title abstract description 13
- 235000015468 Lycium chinense Nutrition 0.000 title abstract description 9
- 235000013399 edible fruits Nutrition 0.000 title abstract description 9
- 108090000790 Enzymes Proteins 0.000 claims abstract description 14
- 102000004190 Enzymes Human genes 0.000 claims abstract description 14
- 239000008280 blood Substances 0.000 claims abstract description 11
- 210000004369 blood Anatomy 0.000 claims abstract description 11
- 238000003809 water extraction Methods 0.000 claims abstract description 9
- 238000000605 extraction Methods 0.000 claims abstract description 8
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 claims abstract description 6
- 150000002632 lipids Chemical class 0.000 claims abstract description 6
- 229940069338 potassium sorbate Drugs 0.000 claims abstract description 6
- 235000010241 potassium sorbate Nutrition 0.000 claims abstract description 6
- 239000004302 potassium sorbate Substances 0.000 claims abstract description 6
- 230000036039 immunity Effects 0.000 claims abstract description 5
- 238000012545 processing Methods 0.000 claims abstract description 4
- 235000013616 tea Nutrition 0.000 claims description 18
- 239000012530 fluid Substances 0.000 claims description 14
- 229940088598 enzyme Drugs 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 7
- 238000010792 warming Methods 0.000 claims description 6
- 239000004480 active ingredient Substances 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- 239000000284 extract Substances 0.000 claims description 4
- 238000011084 recovery Methods 0.000 claims description 4
- 108010059892 Cellulase Proteins 0.000 claims description 3
- 229940106157 cellulase Drugs 0.000 claims description 3
- 239000006166 lysate Substances 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 230000003750 conditioning effect Effects 0.000 claims description 2
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract description 2
- 235000013305 food Nutrition 0.000 abstract description 2
- 239000008103 glucose Substances 0.000 abstract description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 abstract 2
- 239000000706 filtrate Substances 0.000 abstract 2
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 abstract 1
- 229930003268 Vitamin C Natural products 0.000 abstract 1
- FTSSQIKWUOOEGC-RULYVFMPSA-N fructooligosaccharide Chemical compound OC[C@H]1O[C@@](CO)(OC[C@@]2(OC[C@@]3(OC[C@@]4(OC[C@@]5(OC[C@@]6(OC[C@@]7(OC[C@@]8(OC[C@@]9(OC[C@@]%10(OC[C@@]%11(O[C@H]%12O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%12O)O[C@H](CO)[C@@H](O)[C@@H]%11O)O[C@H](CO)[C@@H](O)[C@@H]%10O)O[C@H](CO)[C@@H](O)[C@@H]9O)O[C@H](CO)[C@@H](O)[C@@H]8O)O[C@H](CO)[C@@H](O)[C@@H]7O)O[C@H](CO)[C@@H](O)[C@@H]6O)O[C@H](CO)[C@@H](O)[C@@H]5O)O[C@H](CO)[C@@H](O)[C@@H]4O)O[C@H](CO)[C@@H](O)[C@@H]3O)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@@H](O)[C@@H]1O FTSSQIKWUOOEGC-RULYVFMPSA-N 0.000 abstract 1
- 229940107187 fructooligosaccharide Drugs 0.000 abstract 1
- 235000019154 vitamin C Nutrition 0.000 abstract 1
- 239000011718 vitamin C Substances 0.000 abstract 1
- 238000005516 engineering process Methods 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- 208000001953 Hypotension Diseases 0.000 description 1
- SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 description 1
- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 description 1
- 244000241872 Lycium chinense Species 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- SRBFZHDQGSBBOR-LECHCGJUSA-N alpha-D-xylose Chemical compound O[C@@H]1CO[C@H](O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-LECHCGJUSA-N 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000010100 anticoagulation Effects 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 206010016256 fatigue Diseases 0.000 description 1
- -1 galactolipin Chemical compound 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 208000021822 hypotensive Diseases 0.000 description 1
- 230000001077 hypotensive effect Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 229960003487 xylose Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/166—Addition of, or treatment with, enzymes or microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Medicines Containing Plant Substances (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
The invention relates to an extracting and processing method of green tea and wolfberry fruit polysaccharides and belongs to the technical field of food processing. The green tea and wolfberry fruit polysaccharides are extracted respectively by combining traditional water extraction with modern enzyme engineering; the extraction rates of the green tea and wolfberry fruit polysaccharides can be effectively improved and effective components of the polysaccharides can be kept as much as possible; and vitamin C, fructo-oligosaccharide, potassium sorbate and the like are added into the polysaccharides to prepare a beverage with the functions of improving the immunity, lowering blood lipid and lowering blood glucose. The extracting and processing method comprises the following steps: 1, extracting green tea polysaccharide filtrate; 2, extracting wolfberry fruit polysaccharide filtrate; and 3, producing the green tea and wolfberry fruit polysaccharide beverage.
Description
Technical field
The present invention relates to the extraction of a kind of green tea and LBP-X and processing method, belong to food processing technology field.
Background technology
Tea polysaccharide is a kind of physiological activator having Development volue in tealeaves! Modern pharmacological research finds, tea polysaccharide has hypoglycemic, reducing blood lipid, the hypotensive and effect such as reducing heart rate, resist oxygen lack, is formed, protection blood phase and strengthen in human body non-specific immune function etc. and also have obvious effect at anticoagulation, preventing thrombosis.
LBP-X is a kind of water-soluble polysaccharide extracting from matrimony vine and obtain.Clearly this polysaccharide system proteoglycan, be made up of arabinose, glucose, galactolipin, mannose, wood sugar, rhamnose 6 kinds of monosaccharide components. show after deliberation, the main active that LBP-X is fruit of Chinese wolfberry immunity moderation, delay senility, the symptoms such as the elderly's fatiguability, poor appetite and eye-blurred can be improved, and there is reducing blood lipid, AFL, the anti-ageing effect of waiting for a long time.
The present invention adopts enzyme technology in conjunction with the method for water extraction polysaccharide, effectively extracts the polysaccharide of green tea and matrimony vine, and enzyme engineering technology is in recent years for a biotechnology of active ingredient of natural plant extraction.Select appropriate enzyme, more leniently plant tissue can be decomposed, accelerate the release of active ingredient, thus improve its recovery rate.
The present invention extracts green tea and LBP-X respectively in conjunction with traditional water extraction and modern enzyme engineering, effectively can improve the recovery rate of green tea and LBP-X, and retain the active ingredient of its polysaccharide as far as possible, comprehensively add Vc, FOS and potassium sorbate etc. and make the beverage having and improve immunity, reducing blood lipid, function of blood sugar reduction.
Summary of the invention
The present invention extracts green tea and LBP-X respectively in conjunction with traditional water extraction and modern enzyme engineering, effectively can improve the recovery rate of green tea and LBP-X, and retain the active ingredient of its polysaccharide as far as possible, comprehensively add Vc, FOS and potassium sorbate etc. and make the beverage having and improve immunity, reducing blood lipid, function of blood sugar reduction.Summary of the invention comprises: 1. the extraction of Polysaccharide from Green Tea filtered fluid; 2. the extraction of LBP-X filtered fluid; 3. the making of green tea LBP-X beverage.
Detailed description of the invention
1. tea powder makes: 1) pluck fresh green tea fresh leaves, after the downright bad blade in place to go and stalk, dries 3-4 hour; 2) complete and be dried to water content less than 8%; 3) pulverize, the dry rear tealeaves that completes adopts pulverizer to be ground into 45 object tea powders.
2. enzyme carries tea polysaccharide: 1) tea powder and water are mixed by 1:10; 2) 35-40 DEG C is warming up to; 3) cellulase of 0.01% concentration is added, enzymolysis 30 minutes; 4) 85 DEG C of enzymes that go out are warming up to.
3. water extraction tea polysaccharide: the enzymolysis tea polysaccharide lysate 1) obtained 2, to add water mixing according to 1:2; 2) mixed liquor is heated to 60 DEG C 80 minutes; 3) filter, 200 mesh filter screens filter, and be divided into filtered fluid and filter residue, filtered fluid is the working substance being rich in tea polysaccharide.
4. matrimony vine selection and preliminary working: 1) get lycium barbarum, screening cleaning; 2) dry, cleaned matrimony vine is put into green-keeping machine stir-fry, dry, between dry temperature 150-200 DEG C, time 3-5 minute, is dried to moisture less than 10%; 3) pulverize, dried wolfberry fruit powder is broken into 10 order particles.
5. enzyme carries LBP-X: 1) wolfberry fruit powder and water are mixed by 1:15; 2) 40 DEG C are warming up to; 3) cellulase of 0.01% concentration is added, enzymolysis 30 minutes; 4) 85 DEG C of enzymes that go out are warming up to.
6. water extraction LBP-X: 1) by enzymolysis tea polysaccharide lysate obtained above, to add water mixing according to 1:2; 2) mixed liquor is heated to 60 DEG C 80 minutes; 3) filter, 200 mesh filter screens filter, and filtered fluid is the working substance being rich in LBP-X.
7. the above-mentioned Polysaccharide from Green Tea filtered fluid of beverage blends solution ratio and LBP-X filtered fluid mixing ratio are=1:3.
8. step 7 mixed liquor is added Vc, FOS and potassium sorbate etc., addition is respectively: 0.01%, 1%, 0.03%.
9., by the beverage after above-mentioned interpolation taste conditioning agent, after sterilization processing, pour into into the beverage product of different size.
Claims (5)
1. the processing method of a green tea and LBP-X beverage, it is characterized in that: extract green tea and LBP-X respectively in conjunction with traditional water extraction and modern enzyme engineering, effectively can improve the recovery rate of green tea and LBP-X, and retain the active ingredient of its polysaccharide as far as possible, comprehensively add Vc, FOS and potassium sorbate etc. and make the beverage having and improve immunity, reducing blood lipid, function of blood sugar reduction; Summary of the invention comprises: 1. the extraction of Polysaccharide from Green Tea filtered fluid; 2. the extraction of LBP-X filtered fluid; 3. the making of green tea LBP-X beverage.
2. the method according to claims 1, is characterized in that: the extraction of described Polysaccharide from Green Tea filtered fluid refers to: 1) pluck fresh green tea fresh leaves, after the downright bad blade in place to go and stalk, dries 3-4 hour; 2) complete and be dried to water content less than 8%; 3) pulverize, the dry rear tealeaves that completes adopts pulverizer to be ground into 45 object tea powders; 4) enzyme carries tea polysaccharide; 5) water extraction tea polysaccharide.
3. the method according to claims 2, is characterized in that: described enzyme is carried tea polysaccharide and referred to: 1) tea powder and water are mixed by 1:10; 2) 35-40 DEG C is warming up to; 3) cellulase of 0.01% concentration is added, enzymolysis 30 minutes; 4) 85 DEG C of enzymes that go out are warming up to.
4. the method according to claims 2, is characterized in that: described water extraction tea polysaccharide refers to: 1) by enzymolysis tea polysaccharide lysate, to add water mixing according to 1:2; 2) mixed liquor is heated to 60 DEG C 80 minutes; 3) filter, 200 mesh filter screens filter, and be divided into filtered fluid and filter residue, filtered fluid is the working substance being rich in tea polysaccharide.
5. the method according to claims 1, is characterized in that: the making of described green tea LBP-X beverage refers to: 1) above-mentioned Polysaccharide from Green Tea filtered fluid and LBP-X filtered fluid mixing ratio are=1:3; 2) step 7 mixed liquor is added Vc, FOS and potassium sorbate etc., addition is respectively: 0.01%, 1%, 0.03%; 3) by the beverage after above-mentioned interpolation taste conditioning agent, after sterilization processing, the beverage product of different size is poured into into.
Priority Applications (1)
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CN201510875274.XA CN105410257A (en) | 2015-12-03 | 2015-12-03 | Processing method of green tea and wolfberry fruit polysaccharide beverage |
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CN201510875274.XA CN105410257A (en) | 2015-12-03 | 2015-12-03 | Processing method of green tea and wolfberry fruit polysaccharide beverage |
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CN201510875274.XA Pending CN105410257A (en) | 2015-12-03 | 2015-12-03 | Processing method of green tea and wolfberry fruit polysaccharide beverage |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108522713A (en) * | 2018-04-09 | 2018-09-14 | 中南大学 | A kind of disposable prebiotics black tea and preparation method thereof |
CN111944072A (en) * | 2020-08-20 | 2020-11-17 | 宋现伟 | A kind of wolfberry polysaccharide extraction method, wolfberry polysaccharide tablet and preparation method thereof |
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CN1425452A (en) * | 2003-01-16 | 2003-06-25 | 上海寿祺多糖食品研究所 | Longan aril and wolfberry fruit polysaccharide health-care liquid and its preparing method and use |
CN101167590A (en) * | 2007-12-03 | 2008-04-30 | 黄宝生 | Beverage containing tea polysaccharide |
CN101720830A (en) * | 2008-10-30 | 2010-06-09 | 广州健美乐生物科技有限公司 | Biological polysaccharide medicinal tea beverage |
CN102948838A (en) * | 2011-08-22 | 2013-03-06 | 天津尖峰弗兰德医药科技发展有限公司 | Lycium barbarum polysaccharide contained healthcare beverage and its preparation method |
CN104872775A (en) * | 2015-04-28 | 2015-09-02 | 天津科技大学 | Compound type weight-losing solid beverage with green-tea polysaccharide and application of compound type weight-losing solid beverage |
-
2015
- 2015-12-03 CN CN201510875274.XA patent/CN105410257A/en active Pending
Patent Citations (5)
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CN1425452A (en) * | 2003-01-16 | 2003-06-25 | 上海寿祺多糖食品研究所 | Longan aril and wolfberry fruit polysaccharide health-care liquid and its preparing method and use |
CN101167590A (en) * | 2007-12-03 | 2008-04-30 | 黄宝生 | Beverage containing tea polysaccharide |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108522713A (en) * | 2018-04-09 | 2018-09-14 | 中南大学 | A kind of disposable prebiotics black tea and preparation method thereof |
CN108522713B (en) * | 2018-04-09 | 2021-04-09 | 中南大学 | Washing-free prebiotics dark tea and preparation method thereof |
CN111944072A (en) * | 2020-08-20 | 2020-11-17 | 宋现伟 | A kind of wolfberry polysaccharide extraction method, wolfberry polysaccharide tablet and preparation method thereof |
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