CN105647993A - Production technology of liquid oligogalacturonic acid pectin - Google Patents
Production technology of liquid oligogalacturonic acid pectin Download PDFInfo
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- CN105647993A CN105647993A CN201610102615.4A CN201610102615A CN105647993A CN 105647993 A CN105647993 A CN 105647993A CN 201610102615 A CN201610102615 A CN 201610102615A CN 105647993 A CN105647993 A CN 105647993A
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- pectin
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- galacturonic acid
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- 229920001277 pectin Polymers 0.000 title claims abstract description 29
- 235000010987 pectin Nutrition 0.000 title claims abstract description 29
- 239000001814 pectin Substances 0.000 title claims abstract description 29
- 239000007788 liquid Substances 0.000 title claims abstract description 22
- 239000002253 acid Substances 0.000 title abstract description 12
- 238000004519 manufacturing process Methods 0.000 title abstract description 3
- 238000000034 method Methods 0.000 claims abstract description 14
- 108010059820 Polygalacturonase Proteins 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000000919 ceramic Substances 0.000 claims abstract description 4
- 238000000605 extraction Methods 0.000 claims abstract description 4
- 238000011049 filling Methods 0.000 claims abstract description 4
- 239000012535 impurity Substances 0.000 claims abstract description 4
- 239000012528 membrane Substances 0.000 claims abstract description 4
- 239000002245 particle Substances 0.000 claims abstract description 4
- 238000000926 separation method Methods 0.000 claims abstract description 4
- AEMOLEFTQBMNLQ-YMDCURPLSA-N D-galactopyranuronic acid Chemical compound OC1O[C@H](C(O)=O)[C@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-YMDCURPLSA-N 0.000 claims description 14
- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 claims description 14
- 230000008569 process Effects 0.000 claims description 7
- 108010059892 Cellulase Proteins 0.000 claims description 3
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims description 3
- 229940106157 cellulase Drugs 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 229910017604 nitric acid Inorganic materials 0.000 claims description 3
- 238000003825 pressing Methods 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 abstract description 8
- 102000004190 Enzymes Human genes 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 5
- 150000002772 monosaccharides Chemical class 0.000 abstract description 2
- 230000000593 degrading effect Effects 0.000 abstract 1
- 108010093305 exopolygalacturonase Proteins 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- FGHSTPNOXKDLKU-UHFFFAOYSA-N nitric acid;hydrate Chemical compound O.O[N+]([O-])=O FGHSTPNOXKDLKU-UHFFFAOYSA-N 0.000 abstract 1
- 235000013305 food Nutrition 0.000 description 13
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 description 7
- 229940088598 enzyme Drugs 0.000 description 7
- 229930182830 galactose Natural products 0.000 description 7
- 239000005452 food preservative Substances 0.000 description 3
- 235000019249 food preservative Nutrition 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 230000000845 anti-microbial effect Effects 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 231100000957 no side effect Toxicity 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- FJKROLUGYXJWQN-UHFFFAOYSA-M 4-hydroxybenzoate Chemical compound OC1=CC=C(C([O-])=O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-M 0.000 description 1
- 241000186000 Bifidobacterium Species 0.000 description 1
- 241000675108 Citrus tangerina Species 0.000 description 1
- 208000004930 Fatty Liver Diseases 0.000 description 1
- 206010019708 Hepatic steatosis Diseases 0.000 description 1
- 108010029182 Pectin lyase Proteins 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 150000002016 disaccharides Chemical class 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000001804 emulsifying effect Effects 0.000 description 1
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 description 1
- 208000010706 fatty liver disease Diseases 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 238000009920 food preservation Methods 0.000 description 1
- 235000011194 food seasoning agent Nutrition 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000000413 hydrolysate Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 150000004702 methyl esters Chemical class 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 239000010178 pectin extract Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000033458 reproduction Effects 0.000 description 1
- 235000010199 sorbic acid Nutrition 0.000 description 1
- 239000004334 sorbic acid Substances 0.000 description 1
- 229940075582 sorbic acid Drugs 0.000 description 1
- 231100000240 steatosis hepatitis Toxicity 0.000 description 1
- 235000021147 sweet food Nutrition 0.000 description 1
- 150000003538 tetroses Chemical class 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a production technology of liquid oligogalacturonic acid pectin. Peels are crushed into particles of 5 cm, washed by hot water at 80 DEG C for impurity removal and subjected to a nitric acid water bath to reach the temperature of 75 DEG C and the PH of 1.5, a pectin solution is subjected to vacuum extraction, solid-liquid separation is performed by a press separator, zymolysis is performed, refined filtration and concentration are performed by a ceramic membrane, filling is performed, and the liquid oligogalacturonic acid pectin is obtained. Pectinase adopted in the technology has the characteristics of high enzyme activity and capability of quickly and efficiently degrading pectin, and the method for preparing monosaccharide and oligogalacturonic acid is provided.
Description
Technical field
The present invention relates to pectin production technical field, specifically belong to a kind of liquid oligomeric galacturonic acid process for producing pectin.
Background technology
Galacturonic acid is a monosaccharide units of pectin composition, does not still utilize enzyme method to obtain the method for this kind of monomer at present. Pectin is as a kind of polysaccharide, general polygalacturonase hydrolysis of pectin obtains oligomeric galactose aldehydic acid (2-10 monose), such as HuilinWang, Characterizationandhigh-levelexpressionofametagenome-der ivedalkalinepectatelyaseinrecombinantEscherichiacoli, ProcessBiochemistry, 2014, utilize polygalacturonase hydrolysate to be that disaccharides is to tetrose. If obtaining monose, still needing to be utilized disaccharide-hydrolysing enzymes to be hydrolyzed further could to obtain monose. The concept of oligose puts forward by B.Helgerich etc. nineteen thirty, is the low polymerization sugar of the straight or branched being made up of 2��10 monose. It is the organic compound that in nature, a class has multiple special biological function, and distribution is extensive especially, and of a great variety, occupies sizable proportion in glycobiology function. Oligose comprises two big classes, i.e. natural oligose and functional oligose, oligomeric galactose aldehydic acid belongs to functional oligose, because lack the enzyme system of decomposition function oligose in human gastrointestinal tract inside, it can not be degraded by human body acid, gastric enzyme simultaneously, directly can enter in large intestine and preferentially be utilized by bifidus bacillus, be the factor of the propagation bifidus bacillus of excellent property. Functional oligose also has some characteristics of carbohydrate, and alternative sugar material is as the seasoning matter of sweet food. Oligomeric galactose aldehydic acid has promotion intestinal bifidobacteria and breeds in a large number, coordinate plant growth is grown, anticancer, reduce cholesterol, suppress the effect such as fatty liver (Qian Lili, the fermentation of pectin lyase and the application at the unsaturated oligomeric galactose aldehydic acid of preparation thereof, Zhejiang Polytechnical University's master thesis, in June, 2007), therefore, its application potential is huge. Pectin is a class natural high moleculer eompound, and it is the important component of vegetable cell interstitial, becomes with its methyl esters condensation primarily of galacturonic acid. Pectin extracts usually from the pericarp of oranges and tangerines and apple. Pectin has good gelationization and stable emulsifying effect, extensively for food, medicine, daily use chemicals and textile industry. Anticorrosion and the anti-oxidant problem of food is the key of Food preservation, and wherein anticorrosion problem is more crucial. Because food itself is containing a large amount of nutritive substances and moisture, it is applicable to very much microorganism growth. If food preserves improper, just have a large amount of microbial reproductions, cause food spoilage. It is estimated that the food that the whole world has 10%-20% every year comes from microbial spoilage. Food adds food preservatives, is the method easily and effectively extending effective period of food quality, is thus widely used in the food industry. The food preservatives used in foodstuffs industry mainly contains three major types: phenylformic acid and salt, Sorbic Acid and salt thereof and p-Hydroxybenzoate. They are all chemosynthesis class additives, apply in the food industry because of cheap, favorable anti-corrosion effect at most. But the local flavor of food in use can be produced certain detrimentally affect by the food preservatives of these chemosynthesis more or less. If improper use, also human body can be produced toxic side effect.
Summary of the invention
For the problems referred to above, it is an object of the invention to provide a kind of liquid oligomeric galacturonic acid process for producing pectin, the polygalacturonase adopted has high enzyme and lives, and can the feature of depolymerized pectin rapidly and efficiently, it provides a kind of prepare monose and the method for oligomeric galactose aldehydic acid.
The technical solution used in the present invention is as follows:
A kind of liquid oligomeric galacturonic acid process for producing pectin, comprises the following steps 1) take certain quantity of fresh or dry pericarp is crushed to 5CM particle, �� hot-water soak washing removing impurities that adds 80;
2) water washed apple residue is put into extractor, adds nitric acid water-bath, make pH value be 1.5, temperature 75 ��, vacuum hydro-extraction pectin solution;
3) solid-liquid separation is carried out by pressing to disembark;
4) by 3) solid pomace add the polygalacturonase solution of 2L mass percent 1% with every kilogram, fully mixing rapidly, 50 DEG C of enzymolysis 40 minutes in water-bath;
5) it is 60 orders, 100 orders and 200 object multi-layer silk screen strainers by aperture successively by enzymolysis solution;
6) filtered liquid is placed in ceramic membrane essence filter concentrated, filling obtains liquid oligomeric galacturonic acid pectin.
Described polygalacturonase is cellulase.
Compared with the prior art, the useful effect of the present invention is as follows:
The polygalacturonase that the present invention adopts has high enzyme and lives, and can the feature of depolymerized pectin rapidly and efficiently, it provides a kind of prepare monose and the method for oligomeric galactose aldehydic acid; Liquid oligomeric galacturonic acid pectin has no side effect, and antimicrobial spectrum is wide, it is possible to be widely used in the sanitas of multiple field of food.
Embodiment
A kind of liquid oligomeric galacturonic acid process for producing pectin, comprises the following steps
1) certain quantity of fresh is taken or dry pericarp is crushed to 5CM particle, �� hot-water soak washing removing impurities that adds 80;
2) water washed apple residue is put into extractor, adds nitric acid water-bath, make pH value be 1.5, temperature 75 ��, vacuum hydro-extraction pectin solution;
3) solid-liquid separation is carried out by pressing to disembark;
4) by 3) solid pomace add the polygalacturonase solution of 2L mass percent 1% with every kilogram, fully mixing rapidly, 50 DEG C of enzymolysis 40 minutes in water-bath; Described polygalacturonase is cellulase;
5) it is 60 orders, 100 orders and 200 object multi-layer silk screen strainers by aperture successively by enzymolysis solution;
6) filtered liquid is placed in ceramic membrane essence filter concentrated, filling obtains liquid oligomeric galacturonic acid pectin. The polygalacturonase that the present invention adopts has high enzyme and lives, and can the feature of depolymerized pectin rapidly and efficiently, it provides a kind of prepare monose and the method for oligomeric galactose aldehydic acid; Liquid oligomeric galacturonic acid pectin has no side effect, and antimicrobial spectrum is wide, it is possible to be widely used in the sanitas of multiple field of food.
More than show and describe the ultimate principle of the present invention, the advantage of main characteristic sum the present invention. The technician of the industry should understand; the present invention is not restricted to the described embodiments; the principle that the present invention is just described described in above-described embodiment and specification sheets; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention. The claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (2)
1. a liquid oligomeric galacturonic acid process for producing pectin, it is characterised in that: comprise the following steps 1) take certain quantity of fresh or dry pericarp is crushed to 5CM particle, �� hot-water soak washing removing impurities that adds 80;
2) water washed apple residue is put into extractor, adds nitric acid water-bath, make pH value be 1.5, temperature 75 ��, vacuum hydro-extraction pectin solution;
3) solid-liquid separation is carried out by pressing to disembark;
4) by 3) solid pomace add the polygalacturonase solution of 2L mass percent 1% with every kilogram, fully mixing rapidly, 50 DEG C of enzymolysis 40 minutes in water-bath;
5) it is 60 orders, 100 orders and 200 object multi-layer silk screen strainers by aperture successively by enzymolysis solution;
6) filtered liquid is placed in ceramic membrane essence filter concentrated, filling obtains liquid oligomeric galacturonic acid pectin.
2. a kind of liquid oligomeric galacturonic acid process for producing pectin according to claim 1, it is characterised in that: described polygalacturonase is cellulase.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610102615.4A CN105647993A (en) | 2016-02-24 | 2016-02-24 | Production technology of liquid oligogalacturonic acid pectin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610102615.4A CN105647993A (en) | 2016-02-24 | 2016-02-24 | Production technology of liquid oligogalacturonic acid pectin |
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| Publication Number | Publication Date |
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| CN105647993A true CN105647993A (en) | 2016-06-08 |
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| CN201610102615.4A Pending CN105647993A (en) | 2016-02-24 | 2016-02-24 | Production technology of liquid oligogalacturonic acid pectin |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109619591A (en) * | 2018-12-07 | 2019-04-16 | 浙江工业大学 | A kind of fingered citron pectin oligosaccharide and its preparation and application |
| CN109879985A (en) * | 2019-03-20 | 2019-06-14 | 广州市莱檬生物科技有限公司 | A kind of preparation method of medicinal small molecule pectin |
| WO2023036219A1 (en) * | 2021-09-08 | 2023-03-16 | 上海见杉医学技术有限公司 | Homogalacturonan, complex, and preparation method therefor and use thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103214594A (en) * | 2013-04-16 | 2013-07-24 | 安徽宇宁生物科技有限公司 | Process for producing liquid pectin |
| CN104593450A (en) * | 2015-01-26 | 2015-05-06 | 湖北大学 | Method for splitting pectin with high esterification degree to prepare monosaccharide and oligogalacturonic acid employing pectinase |
-
2016
- 2016-02-24 CN CN201610102615.4A patent/CN105647993A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103214594A (en) * | 2013-04-16 | 2013-07-24 | 安徽宇宁生物科技有限公司 | Process for producing liquid pectin |
| CN104593450A (en) * | 2015-01-26 | 2015-05-06 | 湖北大学 | Method for splitting pectin with high esterification degree to prepare monosaccharide and oligogalacturonic acid employing pectinase |
Non-Patent Citations (2)
| Title |
|---|
| 张蕊: "利用果皮渣制备功能性低聚糖及抑菌应用研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
| 邸铮: "苹果皮渣中果胶的提取及性质研究", 《中国学位论文全文数据库》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109619591A (en) * | 2018-12-07 | 2019-04-16 | 浙江工业大学 | A kind of fingered citron pectin oligosaccharide and its preparation and application |
| CN109879985A (en) * | 2019-03-20 | 2019-06-14 | 广州市莱檬生物科技有限公司 | A kind of preparation method of medicinal small molecule pectin |
| CN109879985B (en) * | 2019-03-20 | 2021-03-19 | 广州市莱檬生物科技有限公司 | Preparation method of medicinal micromolecule pectin |
| WO2023036219A1 (en) * | 2021-09-08 | 2023-03-16 | 上海见杉医学技术有限公司 | Homogalacturonan, complex, and preparation method therefor and use thereof |
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Address after: 235323 Anhui city of Suzhou province Dangshan County Guan Di Miao Zhen one kilometer north (Highway 101 West Road) Applicant after: Anhui Yu Ning pectin Limited by Share Ltd Address before: 235323 Anhui city of Suzhou province Dangshan County Guan Di Miao Zhen one kilometer north (Highway 101 West Road) Applicant before: Anhui Yuning Biotechnology Co., Ltd. |
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