CN104725522A - Method for extracting white fungus polysaccharides at high temperature under high pressure - Google Patents
Method for extracting white fungus polysaccharides at high temperature under high pressure Download PDFInfo
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- CN104725522A CN104725522A CN201510149916.8A CN201510149916A CN104725522A CN 104725522 A CN104725522 A CN 104725522A CN 201510149916 A CN201510149916 A CN 201510149916A CN 104725522 A CN104725522 A CN 104725522A
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Abstract
The invention provides a method for extracting white fungus polysaccharides at high temperature under high pressure, which comprises the following steps: (1) selecting insect-free white fungus, pulverizing in a pulverizer, passing through a 40-mesh screen, and drying in a drying machine; (2) adding a distilled water extractant into the container containing white fungus dry powder in a material/liquid ratio of 1:60-1:80, sealing with parchment paper, and extracting in a 100-120-DEG C water bath and a high-pressure sterilizer under the pressure of 0.2-0.6 MPa respectively for 2 hours; and (3) after finishing extraction, extruding the extracting solution through filter cloth to filter out impurities, and centrifuging in a centrifuge at the speed of 4000 r/min. The extraction method has the characteristics of high extraction yield, high purity, short time consumption and the like, is simple and easy to operate, and is suitable for industrial production.
Description
Technical field
The present invention relates to a kind of method extracting polysaccharide, be specifically related to a kind of method that High Temperature High Pressure gets tremella polysaccharide.
Background technology
White fungus is also known as making Tremella, tremella etc., and belonging to Mycophyta Tremellaceae Tremella, is the sporophore of white fungus, has the laudatory title of " hat in bacterium ".Tremella polysaccharide is a kind of active polysaccharide extracted from Tremella fructification or spore, modern structure analytical proof tremella polysaccharide is a kind of mixed polysaccharide, its main chain is the mannosans be made up of α-(1-3) glycosidic link, 2,4,6 of main chain are connected with the side chain of the residue compositions such as glucose, wood sugar, Fucose and table sugar aldehydic acid, its active centre is this common structure part of α-(1-3) mannosans.Large quantity research proves both at home and abroad, tremella polysaccharide has the physiological actions such as excellent raising human immunologic function, antiviral, antitumor, antiulcer agent, reducing blood-fat, hypoglycemic, decreasing cholesterol, and tremella polysaccharide has extremely strong performance of keeping humidity and thickening stabilities, it is applied in household chemicals field as natural moisture preserving agent and is paid attention to widely.
At present, the traditional method extracting tremella polysaccharide has: hot water extraction method, enzyme process extraction, ultrasound assisted extraction method etc., but due to the four-layer structure that Tremella fructification cell walls has protein, polysaccharide combines, these traditional extracting method are difficult to destroy cell wall structure, fully discharge fruitbody polysaccharide composition, there is the shortcomings such as extraction yield is low, impurity is many.
Summary of the invention
For the deficiencies in the prior art, the object of this invention is to provide a kind of high, consuming time short, the method that High Temperature High Pressure that purity is high extracts tremella polysaccharide of extraction yield.
In order to achieve the above object, the technical scheme that the present invention takes is:
A kind of High Temperature High Pressure extracts tremella polysaccharide volume method, comprises the following steps:
(1) select the white fungus without insect pest, be placed in pulverizer and pulverize, after crossing 40 mesh sieves, be placed in dryer and dry, for subsequent use;
(2) be that 1:60 ~ 1:80 adds distilled water extraction agent to the container being loaded with white fungus dry powder with solid-liquid ratio, with template sealing, to be placed in temperature be respectively water-bath, the pressure of 100 ~ 120 DEG C is 0.2 ~ 0.6MP
aautoclave in all extract 2h;
(3) after time up to be extracted, extracting solution filter cloth press filtration goes out decon, is placed in the centrifuge that rotating speed is 4000r/min, can obtains white fungus raw sugar.
As optimization technique, in order to ensure the extraction conditions of tremella polysaccharide optimum, to ensure high extraction, high purity, described step adds distilled water extraction agent with the solid-liquid ratio of 1:70 in (2).
As optimization technique, in order to ensure the extraction conditions of tremella polysaccharide optimum, to ensure high extraction, high purity, in described step (2), the temperature of water-bath is 110 DEG C.
As optimization technique, in order to ensure the extraction conditions of tremella polysaccharide optimum, to ensure high extraction, high purity, the pressure of described step (2) mesohigh Sterilizers is 0.4MP
a.
Beneficial effect of the present invention: take High Temperature High Pressure to extract tremella polysaccharide, improve cell-penetrating power and the cytoclasis power of Extraction solvent, effectively can accelerate the release of polysaccharide, high-temperature and high-pressure conditions simultaneously, also can make the easier stripping from cell of tremella polysaccharide, and take optimum extraction process, thus extraction yield is high, purity is high, consuming time short, and this extracting method is simple, easy handling, is suitable for suitability for industrialized production.
Embodiment
Below in conjunction with specific embodiment, the present invention is further described.
Embodiment 1
A kind of High Temperature High Pressure extracts tremella polysaccharide volume method, comprises the following steps:
(1) select the white fungus without insect pest, be placed in pulverizer and pulverize, after crossing 40 mesh sieves, be placed in dryer and dry, for subsequent use;
(2) be that 1:60 adds distilled water extraction agent to the container being loaded with white fungus dry powder with solid-liquid ratio, with template sealing, to be placed in temperature be respectively water-bath, the pressure of 100 DEG C is 0.2MP
aautoclave in all extract 2h;
(3) after time up to be extracted, extracting solution filter cloth press filtration goes out decon, is placed in the centrifuge that rotating speed is 4000r/min, can obtains white fungus raw sugar.
Embodiment 2
A kind of High Temperature High Pressure extracts tremella polysaccharide volume method, comprises the following steps:
(1) select the white fungus without insect pest, be placed in pulverizer and pulverize, after crossing 40 mesh sieves, be placed in dryer and dry, for subsequent use;
(2) be that 1:65 adds distilled water extraction agent to the container being loaded with white fungus dry powder with solid-liquid ratio, with template sealing, to be placed in temperature be respectively water-bath, the pressure of 105 DEG C is 0.3MP
aautoclave in all extract 2h;
(3) after time up to be extracted, extracting solution filter cloth press filtration goes out decon, is placed in the centrifuge that rotating speed is 4000r/min, can obtains white fungus raw sugar.
Embodiment 3
A kind of High Temperature High Pressure extracts tremella polysaccharide volume method, comprises the following steps:
(1) select the white fungus without insect pest, be placed in pulverizer and pulverize, after crossing 40 mesh sieves, be placed in dryer and dry, for subsequent use;
(2) be that 1:70 adds distilled water extraction agent to the container being loaded with white fungus dry powder with solid-liquid ratio, with template sealing, to be placed in temperature be respectively water-bath, the pressure of 110 DEG C is 0.4MP
aautoclave in all extract 2h;
(3) after time up to be extracted, extracting solution filter cloth press filtration goes out decon, is placed in the centrifuge that rotating speed is 4000r/min, can obtains white fungus raw sugar.
Embodiment 4
A kind of High Temperature High Pressure extracts tremella polysaccharide volume method, comprises the following steps:
(1) select the white fungus without insect pest, be placed in pulverizer and pulverize, after crossing 40 mesh sieves, be placed in dryer and dry, for subsequent use;
(2) be that 1:75 adds distilled water extraction agent to the container being loaded with white fungus dry powder with solid-liquid ratio, with template sealing, to be placed in temperature be respectively water-bath, the pressure of 115 DEG C is 0.5MP
aautoclave in all extract 2h;
(3) after time up to be extracted, extracting solution filter cloth press filtration goes out decon, is placed in the centrifuge that rotating speed is 4000r/min, can obtains white fungus raw sugar.
Embodiment 5
A kind of High Temperature High Pressure extracts tremella polysaccharide volume method, comprises the following steps:
(1) select the white fungus without insect pest, be placed in pulverizer and pulverize, after crossing 40 mesh sieves, be placed in dryer and dry, for subsequent use;
(2) be that 1:80 adds distilled water extraction agent to the container being loaded with white fungus dry powder with solid-liquid ratio, with template sealing, to be placed in temperature be respectively water-bath, the pressure of 120 DEG C is 0.6MP
aautoclave in all extract 2h;
(3) after time up to be extracted, extracting solution filter cloth press filtration goes out decon, is placed in the centrifuge that rotating speed is 4000r/min, can obtains white fungus raw sugar.
It should be pointed out that above case is only the representational example of the present invention.Obviously, technical scheme of the present invention is not limited to above-described embodiment, can also have many sex change.
Claims (4)
1. High Temperature High Pressure extracts a method for tremella polysaccharide, it is characterized in that, comprises the following steps:
(1) select the white fungus without insect pest, be placed in pulverizer and pulverize, after crossing 40 mesh sieves, be placed in dryer and dry, for subsequent use;
(2) be that 1:60 ~ 1:80 adds distilled water extraction agent to the container being loaded with white fungus dry powder with solid-liquid ratio, with template sealing, to be placed in temperature be respectively water-bath, the pressure of 100 ~ 120 DEG C is 0.2 ~ 0.6MP
aautoclave in all extract 2h;
(3) after time up to be extracted, extracting solution filter cloth press filtration goes out decon, is placed in the centrifuge that rotating speed is 4000r/min, can obtains white fungus raw sugar.
2. a kind of High Temperature High Pressure extracts the method for tremella polysaccharide according to claim 1, it is characterized in that: described step adds distilled water extraction agent with the solid-liquid ratio of 1:70 in (2).
3. a kind of High Temperature High Pressure extracts the method for tremella polysaccharide according to claim 1, it is characterized in that: in described step (2), the temperature of water-bath is 110 DEG C.
4. a kind of High Temperature High Pressure extracts the method for tremella polysaccharide according to claim 1, it is characterized in that: the pressure of described step (2) mesohigh Sterilizers is 0.4MP
a.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510149916.8A CN104725522A (en) | 2015-03-31 | 2015-03-31 | Method for extracting white fungus polysaccharides at high temperature under high pressure |
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| CN201510149916.8A CN104725522A (en) | 2015-03-31 | 2015-03-31 | Method for extracting white fungus polysaccharides at high temperature under high pressure |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105348403A (en) * | 2015-10-15 | 2016-02-24 | 西北师范大学 | Method for extracting ultra-white lily polysaccharide from inferior lily bud scales |
| CN106065036A (en) * | 2016-07-19 | 2016-11-02 | 西北农林科技大学 | A kind of hydro-thermal method extracts the method for tea polysaccharide |
| CN107373629A (en) * | 2017-07-24 | 2017-11-24 | 广州市金因源生物技术有限公司 | Dried tremella extract and its extracting method and application |
| CN108041383A (en) * | 2017-12-15 | 2018-05-18 | 龚雪梅 | A kind of high nutrition tremella beverage with white fungus typicalness flavor and preparation method thereof |
| CN113201079A (en) * | 2021-05-13 | 2021-08-03 | 襄阳嘉达生物科技有限公司 | Method for extracting polysaccharide from tremella |
| CN115819638A (en) * | 2022-12-28 | 2023-03-21 | 广东药科大学 | Preparation method of tremella polysaccharides with different molecular weights |
-
2015
- 2015-03-31 CN CN201510149916.8A patent/CN104725522A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 黄晓德等: "银耳子实体多糖高温高压提取工艺研究", 《中国野生植物资源》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105348403A (en) * | 2015-10-15 | 2016-02-24 | 西北师范大学 | Method for extracting ultra-white lily polysaccharide from inferior lily bud scales |
| CN106065036A (en) * | 2016-07-19 | 2016-11-02 | 西北农林科技大学 | A kind of hydro-thermal method extracts the method for tea polysaccharide |
| CN107373629A (en) * | 2017-07-24 | 2017-11-24 | 广州市金因源生物技术有限公司 | Dried tremella extract and its extracting method and application |
| CN107373629B (en) * | 2017-07-24 | 2020-11-10 | 广州市金因源生物技术有限公司 | Tremella extract and extraction method and application thereof |
| CN108041383A (en) * | 2017-12-15 | 2018-05-18 | 龚雪梅 | A kind of high nutrition tremella beverage with white fungus typicalness flavor and preparation method thereof |
| CN108041383B (en) * | 2017-12-15 | 2021-11-09 | 龚雪梅 | High-nutrition tremella beverage with typical tremella flavor and preparation method thereof |
| CN113201079A (en) * | 2021-05-13 | 2021-08-03 | 襄阳嘉达生物科技有限公司 | Method for extracting polysaccharide from tremella |
| CN115819638A (en) * | 2022-12-28 | 2023-03-21 | 广东药科大学 | Preparation method of tremella polysaccharides with different molecular weights |
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