CN107006866A - A kind of preparation method of soluble dietary fiber - Google Patents

Abstract

The present invention relates to a kind of preparation method of soluble dietary fiber, and described method comprises the following steps: (1) pretreatment: beating after the fruit is washed, pulverize and grind, adjust pH and stir to prepare suspension; (2) enzymatic hydrolysis: to step Add enzymes to the suspension in (1), adjust the pH and stir, separate the solid and liquid, collect the supernatant to adjust the pH to neutral; (3) membrane separation: carry out ultrafiltration treatment on the supernatant obtained in step (2), Collect the permeate; (4) Concentrate: Concentrate the permeate obtained in step (3); (5) Spray drying: Spray dry the concentrate obtained in step (4), and the dried powder is soluble dietary fiber . The preparation method of the present invention can extract the dietary fiber of various fruits, and the extracted dietary fiber has a high rate and high biological activity. However, the method of the present invention can specifically extract the dietary fiber from peaches, and the extraction effect is the best. The fiber has the highest yield and the highest biological activity.

Description

A kind of preparation method of soluble dietary fiber

technical field

The invention belongs to the field of food processing, and relates to a method for preparing soluble dietary fiber, in particular to a method for preparing soluble dietary fiber by extracting and enzymatically hydrolyzing peaches as raw materials.

Background technique

Soluble dietary fiber is a type of fiber that is soluble in water, can absorb water and swell, and can be decomposed and utilized by intestinal microorganisms. It often exists in plant cell fluid and intercellular matrix, mainly including pectin, plant glue and viscose. Soluble dietary fiber can effectively activate the beneficial bacteria in the intestinal tract, promote the proliferation of beneficial bacteria, and create a healthy intestinal ecology. Studies in recent years have shown that soluble dietary fiber can affect the expression of related inflammatory cytokines and play a good role in preventing inflammation. There are also many soluble dietary fibers that can effectively reduce blood lipids and cholesterol, prevent cardiovascular and cerebrovascular diseases, and lower blood sugar. , reduce the occurrence of diabetes, anti-inflammation, can also induce tumor cell apoptosis, prevent tissue cancer, etc. Dietary fiber is listed as the "seventh largest nutrient" after sugar, protein, fat, water, minerals and vitamins.

Peach (Prunus persica) is the fruit of the genus Prunus in the family Rosaceae. Its shape includes oval, wide oval or oblate, its color changes from light greenish white to orange yellow, and its pulp is white, light greenish white, yellow, orange yellow or The red, juicy flesh of the peach is fragrant, sweet or tart. Peach pulp is rich in glucose, fructose, protein, vitamins, carotene, calcium, phosphorus, iron and other ingredients; peach has a long history of cultivation in my country and is very popular in domestic and foreign markets. Due to the thin skin of peaches, the picking period is generally in the high temperature season and the harvesting period is relatively concentrated. Under normal temperature conditions, the interior or exterior is perishable and cannot be stored. In addition, peaches are sensitive to temperature and are easy to store under ordinary refrigeration conditions. Phenomena such as chilling injury and flocculation occur, and the pulp is prone to browning or gelatinization, the texture is rough and dry, the color is dark, the juice is sticky, and it is difficult to squeeze the juice, which seriously affects its commodity value and economic benefits. Therefore, peaches are often processed into products such as canned food, peach juice, dried peaches, preserved peaches or peach jam, which can avoid problems such as difficulty in storage to a certain extent. The added value of these products is not high, and the retention of nutrients still needs to be improved. improve.

There is a certain amount of pectin and cellulose in peaches. Cellulose is an important component of the cell wall of peaches. Pectin is an important component of pectin in cells. Soluble dietary fiber can be prepared after proper degradation. Therefore, peaches can be used as raw materials for soluble dietary fiber. Currently disclosed methods for extracting dietary fiber at home and abroad mainly include chemical extraction, enzymatic extraction, chemical-enzyme combined extraction, membrane separation and fermentation. CN101283760A discloses a method for extracting and preparing dietary fiber from peach pomace, said method comprising (1) removing impurities and sterilizing; (2) heat preservation and leaching; (3) solid-liquid separation; (4) concentrating the filtrate, ethanol precipitation , dry after precipitation to obtain soluble dietary fiber; (5) mix filter cake with water, adjust pH, enzymatic hydrolysis, filter to obtain filtrate concentration, alcohol precipitation, precipitation and precipitation to obtain soluble dietary fiber. The method extracts dietary fiber from peach residue, the steps are complicated, and the yield of soluble fiber on the market is not high.

CN102885318A discloses a method for extracting sweet potato dietary fiber, which uses α-amylase, trypsin and glucoamylase to prepare and separate soluble dietary fiber and insoluble dietary fiber, and studies the influence of enzymatic hydrolysis conditions on product yield. The extraction rates are respectively 25.7% and 55.9%; CN103284152A discloses a dietary fiber extracted from Taxus chinensis, its extraction method and its application. The high-pressure homogenization-enzyme combination method is used to extract soluble dietary fiber and insoluble dietary fiber to obtain red bean with high yield Chinese fir insoluble dietary fiber (80-90%), but the content of its soluble dietary fiber is relatively low (10-13%); CN102871134A discloses a kind of method for extracting dietary fiber, after electrolyzed water is extracted, water-bath heating, centrifugation after cooling , concentration, alcohol precipitation and drying to obtain soybean meal soluble and insoluble dietary fiber, the content of which is 14.8% and 53.7% respectively.

These methods are all single and independent, lack of effective combination, resulting in low extraction rate, low product activity, or too complicated process, which is not conducive to realizing industrialization. Therefore, there is an urgent need for a method that can efficiently extract high-activity dietary fiber to obtain soluble dietary fiber with high biological activity.

Contents of the invention

Aiming at the problems that fruits are difficult to store, the added value of products prepared by traditional processing methods is low, and the retention of nutrients is low, the present invention aims to provide a method for preparing soluble dietary fiber using fruits as raw materials. The method has simple steps and the dietary fiber can be obtained High rate.

For reaching this purpose, the present invention adopts following technical scheme:

In a first aspect, the present invention provides a method for preparing soluble dietary fiber, comprising the steps of:

(1) Pretreatment: washing the fruit, beating, pulverizing and grinding, adjusting the pH and stirring to prepare a suspension;

(2) Enzymolysis: add enzyme to the suspension in step (1), adjust the pH and stir, separate the solid and liquid, collect the supernatant and adjust the pH to neutral;

(3) Membrane separation: the supernatant obtained in step (2) is subjected to ultrafiltration, and the permeate is collected;

(4) concentrating: concentrating the permeate obtained in step (3);

(5) Spray drying: the concentrate obtained in step (4) is spray-dried, and the dried powder is soluble dietary fiber.

Preferably, the fruit described in step (1) is any one or a combination of at least two of peaches, apples, bananas or pineapples, preferably peaches.

In the present invention, the peach can be selected from but not limited to honey peach, flat peach or nectarine.

The preparation method of the invention can extract the dietary fiber of various fruits, and the effect of extracting the dietary fiber specifically for peaches is the best, the yield of the dietary fiber is the highest, and the biological activity is the best.

Preferably, the beating described in step (1) specifically includes: adding water 1-10 times the weight of the fruit, performing beating treatment by mechanical means, and separating the fruit pulp and fruit core.

The amount of the water is 1 time, 2 times, 3 times, 4 times, 5 times, 6 times, 7 times, 8 times, 9 times or 10 times of the fruit weight.

Preferably, the crushing and grinding described in step (1) specifically includes: adding water 1-30 times the weight of the fruit to the fruit pulp, and mechanically crushing and grinding the fruit pulp.

The amount of water is 1 times, 2 times, 3 times, 4 times, 5 times, 6 times, 7 times, 8 times, 9 times, 10 times, 11 times, 12 times, 13 times, 14 times of the fruit weight , 15 times, 16 times, 17 times, 18 times, 19 times, 20 times, 21 times, 22 times, 23 times, 24 times, 25 times, 26 times, 27 times, 28 times, 29 times or 30 times.

Preferably, the pH in step (1) is 3-11, such as 3, 4, 5, 6, 7, 8, 9, 10 or 11, preferably 4-7.

Preferably, the stirring temperature in step (1) is 20-80°C, such as 20°C, 21°C, 22°C, 23°C, 25°C, 26°C, 28°C, 30°C, 32°C, 35°C ℃, 38℃, 40℃, 42℃, 45℃, 48℃, 50℃, 52℃, 55℃, 58℃, 60℃, 62℃, 65℃, 68℃, 70℃, 72℃, 75℃, 78°C or 80°C, preferably 30-60°C.

Preferably, the stirring time in step (1) is 1-12h, such as 1h, 2h, 3h, 4h, 5h, 6h, 7h, 8h, 9h, 10h, 11h or 12h, preferably 3-10h.

Preferably, the mass ratio of the enzyme to the fruit in step (2) is 1:(50-10000), such as 1:50, 1:60, 1:70, 1:80, 1:90, 1:100 , 1:120, 1:150, 1:200, 1:250, 1:300, 1:350, 1:400, 1:500, 1:600, 1:700, 1:800, 1:900, 1 :1000, 1:1200, 1:1500, 1:1600, 1:1800, 1:1900, 1:2000, 1:2500, 1:3000, 1:3500, 1:4000, 1:4500, 1:5000 , 1:5500, 1:6000, 1:6500, 1:7000, 1:7500, 1:8000, 1:8500, 1:9000, 1:9500 or 1:10000, preferably 1:(100-8000) , more preferably 1:(500-6000).

Preferably, the enzyme is any one or a combination of at least two of pectinesterase, polygalacturonase, endoglucanase, exoglucanase or β-glucosidase .

Preferably, the pH in step (2) is 2-11, such as 2, 3, 4, 5, 6, 7, 8, 9, 10 or 11, preferably 3-8, more preferably 4-7 .

Preferably, the stirring temperature in step (2) is 10-80°C, such as 10°C, 11°C, 12°C, 13°C, 14°C, 15°C, 16°C, 17°C, 18°C, 19°C ℃, 20℃, 21℃, 22℃, 23℃, 25℃, 26℃, 28℃, 30℃, 32℃, 35℃, 38℃, 40℃, 42℃, 45℃, 48℃, 50℃, 52°C, 55°C, 58°C, 60°C, 62°C, 65°C, 68°C, 70°C, 72°C, 75°C, 78°C or 80°C, preferably 20-60°C.

Preferably, the stirring time described in step (2) is 1-20h, such as 1h, 2h, 3h, 4h, 5h, 6h, 7h, 8h, 9h, 10h, 11h, 12h, 13h, 14h, 15h, 16h, 17h, 18h, 19h or 20h, preferably 3-16h.

Preferably, the ultrafiltration treatment described in step (3) is ultrafiltration using an ultrafiltration membrane.

Preferably, the molecular weight cut-off of the ultrafiltration membrane is 10-500kDa, such as 10kDa, 12kDa, 13kDa, 15kDa, 16kDa, 18kDa, 20kDa, 25kDa, 30kDa, 35kDa, 40kDa, 45kDa, 50kDa, 55kDa, 60kDa, 65kDa , 70kDa, 75kDa, 80kDa, 85kDa, 90kDa, 95kDa, 100kDa, 110kDa, 120kDa, 150kDa, 160kDa, 180kDa, 200kDa, 220kDa, 250kDa, 280kDa, 300kDa, 320kDa, 3Da0kDa, 380kDa, 400kDa, 4500kDa, 4500kDa , preferably 30-400 kDa, more preferably 50-300 kDa.

Preferably, the ultrafiltration temperature is 20-80°C, such as 20°C, 21°C, 22°C, 23°C, 25°C, 26°C, 28°C, 30°C, 32°C, 35°C, 38°C , 40°C, 42°C, 45°C, 48°C, 50°C, 52°C, 55°C, 58°C, 60°C, 62°C, 65°C, 68°C, 70°C, 72°C, 75°C, 78°C or 80°C °C, preferably 30-60 °C.

Preferably, the concentration described in step (4) is concentrated to a solid content of 5-25%, such as 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13% %, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24% or 25%, preferably 10-20%.

In the present invention, the spray drying is a conventional technique in the field, and those skilled in the art can select conditions according to needs, and there is no special limitation here.

In the present invention, the yield of soluble dietary fiber is improved through enzymatic hydrolysis, the product is more uniform through ultrafiltration membrane separation, and the biological activity of extracted soluble dietary fiber is further improved through the cooperation and synergy of the steps of enzymatic hydrolysis and ultrafiltration membrane .

According to the present invention, the preparation method of described soluble dietary fiber comprises the steps:

(1) Pretreatment: After washing the fruit, add water 1-10 times the weight of the fruit for beating, add water 1-30 times the weight of the fruit for crushing and grinding, adjust the pH to 3-11, stir at 20-80°C for 1-12 hours, preparation of the suspension;

(2) Enzymolysis: Add enzymes with a mass ratio of 1:(50-10000) to the suspension in step (1), adjust the pH to 2-11, stir at 10-80°C for 1-20h, solid-liquid Separate and collect the supernatant to adjust the pH to neutral;

(3) Membrane separation: the supernatant obtained in step (2) is subjected to ultrafiltration at 20-80° C. by using an ultrafiltration membrane with a molecular weight cut-off of 10-500 kDa, and the permeate is collected;

(4) concentrating: concentrating the permeate obtained in step (3) to a solid content of 5-25%;

(5) Spray drying: the concentrated solution obtained in step (4) is spray-dried, and the dried powder is soluble dietary fiber;

Wherein, the fruit described in step (1) is any one or a combination of at least two of peach, apple, banana or pineapple, and the enzyme described in step (2) is pectin esterase, polygalacturonase, Any one or a combination of at least two of endoglucanase, exoglucanase or β-glucosidase.

As a preferred technical scheme, the preparation method of the soluble dietary fiber comprises the following steps:

(1) Pretreatment: After cleaning the peaches, add water 2-8 times the weight of the fruit for beating, add water 5-25 times the weight of the fruit for crushing and grinding, adjust the pH to 4-7, stir at 30-60°C for 3-10 hours, preparation of the suspension;

(2) Enzymolysis: Add an enzyme with a mass ratio of 1:(100-8000) to the suspension in step (1), adjust the pH to 3-8, stir at 20-60°C for 3-16h, solid-liquid Separate and collect the supernatant to adjust the pH to neutral;

(3) Membrane separation: the supernatant obtained in step (2) is subjected to ultrafiltration at 30-60° C. by using an ultrafiltration membrane with a molecular weight cut-off of 30-400 kDa, and the permeate is collected;

(4) concentrating: concentrating the permeate obtained in step (3) to a solid content of 10-20%;

(5) Spray drying: the concentrated solution obtained in step (4) is spray-dried, and the dried powder is soluble dietary fiber;

Wherein, the enzyme described in step (2) is any one or at least two of pectin esterase, polygalacturonase, endoglucanase, exoglucanase or β-glucosidase combination of species.

Compared with the prior art, the present invention has at least the following beneficial effects:

The preparation method of the present invention can extract the dietary fiber of various fruits, the yield of the extracted dietary fiber is high, up to 6.3%, the biological activity is high, and the hypoglycemic rate can reach above 30%, and the method of the present invention can specifically The extraction of dietary fiber from peaches has the best extraction effect, the highest yield of dietary fiber, which can reach more than 9%, and the highest biological activity. The method is simple in process, easy to operate, and can be used for industrialized large-scale production.

Instructions attached

Fig. 1 is the flowchart of the preparation method of soluble dietary fiber of the present invention.

detailed description

In order to facilitate understanding of the present invention, the present invention enumerates the following examples. It should be clear to those skilled in the art that the embodiments are only for helping to understand the present invention, and should not be regarded as specific limitations on the present invention.

Example 1

The raw material is freshly picked Shenzhou peaches, 100kg.

(1) Pretreatment: wash 100kg of Shenzhou peaches and add 300kg of pure water, use a peach beater to beat the peaches, filter the peach pulp and peach pits to obtain 385kg of peach pulp, and add 300kg to the peach pulp Purified water, using a colloid mill to deeply pulverize the peach pulp, then adjusting the pH of the feed solution to 4.5, and stirring at 35°C for 3 hours to obtain 685 kg of peach pulp suspension;

(2) Enzymolysis: Add 100 g of pectin esterase to the peach pulp suspension obtained in step (1), adjust the pH to 3.0, stir at 50°C for 3 hours, and collect 660 kg of clear liquid after solid-liquid separation , adjust its pH to 7.0;

(3) Membrane separation: using an ultrafiltration membrane with a molecular weight cut-off of 300kDa, the supernatant liquid obtained in step (2) was subjected to ultrafiltration treatment at 50°C, and the collected permeate was 90kg;

(4) Concentration: Concentrate the permeate obtained in step (3) until the solid content of the solution is 15%;

(5) Spray drying: the concentrate obtained in step (5) is dried to obtain 13.5kg of soluble dietary fiber.

The yield of the soluble dietary fiber is 13.5%. The gained soluble dietary fiber is fed to the hyperglycemia model mice with pancreatic islet damage, and the fasting blood glucose is measured. Compared with the control group (mice without feeding soluble dietary fiber), the hypoglycemic rate was 38%.

Example 2

The raw material is 100kg of mature flat peaches, which are left for 3-5 days after being picked.

(1) Pretreatment: After cleaning 100kg flat peaches, add 200kg pure water, use a peach beater to beat the peaches, filter the peach pulp and peach pits to obtain 280kg peach pulp, add 400kg pure water to the peach pulp, Deeply pulverize the peach pulp with a colloid mill, then adjust the pH of the feed solution to 4.0, and stir at 55°C for 4 hours to obtain a peach pulp suspension;

(2) Enzymolysis: Add polygalacturonase to the peach pulp suspension obtained in step (1), the addition amount is 50g, the pH is adjusted to 4.5, stirred at 55°C for 3 hours, and then Solid-liquid separation, collecting the supernatant and adjusting its pH to 7.0;

(3) Membrane separation: using an ultrafiltration membrane with a molecular weight cut-off of 500kDa, the supernatant liquid obtained in step (2) was subjected to ultrafiltration treatment at 60° C., and the collected permeate was 120 kg;

(4) Concentration: Concentrate the permeate obtained in step (3) until the solid content of the solution is 12%;

(5) Spray drying: the concentrate obtained in step (5) was dried to obtain 14.4kg of soluble dietary fiber.

The yield of the dietary fiber is 14.4%. The obtained soluble dietary fiber is fed to the hyperglycemia model mice with pancreatic islet damage, and the fasting blood glucose is measured. Compared with the control group (mice without feeding the soluble dietary fiber), the hypoglycemic rate is 35%.

Example 3

The raw material is 100kg of mature flat peaches, which are left for 3-5 days after being picked.

(1) Pretreatment: After cleaning 100kg flat peaches, add 100kg pure water, use a peach beater to beat the peaches, filter the peach pulp and peach pits to obtain 160kg peach pulp, continue adding 3000kg pure water to the peach pulp, Deeply pulverize the peach pulp with a colloid mill, then adjust the pH of the feed solution to 3.0, and stir at 80°C for 1 hour to obtain a peach pulp suspension;

(2) Enzymolysis: Add endoglucanase to the peach pulp suspension obtained in step (1), the addition amount is 2000g, the pH is adjusted to 2, stirred at 80°C for 1 hour, and then solidified Liquid separation, collecting the supernatant and adjusting its pH to 7.0;

(3) Membrane separation: using an ultrafiltration membrane with a molecular weight cut-off of 10 kDa, the clear liquid obtained in step (2) was subjected to ultrafiltration at 80° C., and the collected permeate was 105 kg;

(4) Concentration: Concentrate the permeate obtained in step (3) until the solid content of the solution is 5%;

(5) Spray drying: the concentrated solution obtained in step (5) is dried to obtain 10.2 kg of soluble dietary fiber.

The yield of the dietary fiber is 10.2%. The obtained soluble dietary fiber is fed to the hyperglycemia model mice with pancreatic islet damage, and the fasting blood glucose is measured. Compared with the control group (mice without feeding the soluble dietary fiber), the hypoglycemic rate is 31%.

Example 4

The raw material is 100kg of mature flat peaches, which are left for 3-5 days after being picked.

(1) Pretreatment: After cleaning 100kg of flat peaches, add 1000kg of pure water, use a peach beater to beat the peaches, separate the peach pulp and peach pits by filtration, obtain 960kg of peach pulp, continue to add 100kg of pure water to the peach pulp, Deeply pulverize the peach pulp with a colloid mill, then adjust the pH of the feed solution to 11.0, and stir at 20°C for 12 hours to obtain a peach pulp suspension;

(2) Enzymolysis: add β-glucosidase to the peach pulp suspension obtained in step (1), the addition amount is 10g, adjust the pH to 11, stir and treat at 10°C for 20 hours, and then carry out solid-liquid Separate, collect supernatant and adjust its pH to 7.0;

(3) Membrane separation: using an ultrafiltration membrane with a molecular weight cut-off of 500kDa, the supernatant liquid obtained in step (2) was subjected to ultrafiltration treatment at 20°C, and the collected permeate was 830kg;

(4) Concentration: Concentrate the permeate obtained in step (3) until the solid content of the solution is 25%;

(5) Spray drying: the concentrate obtained in step (5) is dried to obtain 90kg of soluble dietary fiber.

The yield of the dietary fiber is 9%. The obtained soluble dietary fiber is fed to the hyperglycemia model mice with pancreatic islet damage, and the fasting blood glucose is measured. Compared with the control group (mice without feeding the soluble dietary fiber), the hypoglycemic rate 32%.

Example 5

The raw material is 100kg of mature pineapples, which are left for 2-3 days after being picked.

(1) Pretreatment: After cleaning 100kg of pineapple, add 200kg of pure water, use a pineapple beater to beat the pineapple, separate the pineapple pulp from the pineapple core by filtration, obtain 260kg of pineapple pulp, and continue to add 400kg of pure water to the pineapple pulp, Deeply pulverize the peach pulp with a colloid mill, then adjust the pH of the feed solution to 4.0, and stir at 55°C for 4 hours to obtain a pineapple pulp suspension;

(2) Enzymolysis: Add polygalacturonase to the pineapple pulp suspension obtained in step (1), the addition amount is 50g, the pH is adjusted to 4.5, stirred at 55°C for 3 hours, and then carried out Solid-liquid separation, collecting the supernatant and adjusting its pH to 7.0;

(3) Membrane separation: use an ultrafiltration membrane with a molecular weight cut-off of 500kDa to carry out ultrafiltration treatment on the clear liquid obtained in step (2) at 60°C, and collect 100kg of permeate;

(4) Concentration: Concentrate the permeate obtained in step (3) until the solid content of the solution is 12%;

(5) Spray drying: the concentrate obtained in step (5) is dried to obtain 8.5kg of soluble dietary fiber.

The yield of the dietary fiber is 8.5%. The obtained soluble dietary fiber is fed to the hyperglycemia model mice with pancreatic islet damage, and the fasting blood glucose is measured. Compared with the control group (mice without feeding the soluble dietary fiber), the hypoglycemic rate is 25%.

Example 6

The raw material is 100kg ripe apples that are left for 3-5 days after being picked.

(1) Pretreatment: Wash 100kg of apples and add 200kg of pure water, use an apple beater to beat the apples, and filter to separate the apple pulp from the apple core to obtain 250kg of apple pulp, and continue to add 400kg of pure water to the apple pulp. Deeply pulverize the apple pulp with a colloid mill, then adjust the pH of the feed solution to 4.0, and stir at 55°C for 4 hours to obtain an apple pulp suspension;

(2) Enzymolysis: Add polygalacturonase to the apple pulp suspension obtained in step (1), in an amount of 50 g, adjust the pH to 4.5, stir and treat for 3 hours at 55° C., and then carry out Solid-liquid separation, collecting the supernatant and adjusting its pH to 7.0;

(3) Membrane separation: using an ultrafiltration membrane with a molecular weight cut-off of 500kDa, the supernatant liquid obtained in step (2) was subjected to ultrafiltration treatment at 60°C, and the permeate was collected to be 80kg;

(4) Concentration: Concentrate the permeate obtained in step (3) until the solid content of the solution is 12%;

(5) Spray drying: the concentrate obtained in step (5) was dried to obtain 6.3kg of soluble dietary fiber.

The yield of the dietary fiber is 6.3%. The obtained soluble dietary fiber is fed to the hyperglycemia model mice with pancreatic islet damage, and the fasting blood sugar is measured. Compared with the control group (mice without feeding the soluble dietary fiber), the hypoglycemic rate 26%.

In summary, the preparation method of the present invention can extract the dietary fiber of various fruits, and the extracted dietary fiber has a high rate and high biological activity, and the method of the present invention can specifically extract the dietary fiber for peach, and the extracted The effect is the best, the yield of dietary fiber is the highest, and the biological activity is the highest.

The applicant declares that the present invention illustrates the technical process of the present invention through the above-mentioned examples, but the present invention is not limited to the above-mentioned detailed process, that is, it does not mean that the present invention can only be implemented depending on the above-mentioned detailed process. Those skilled in the art should understand that any improvement of the present invention, the equivalent replacement of each raw material of the product of the present invention, the addition of auxiliary components, the selection of specific methods, etc., all fall within the scope of protection and disclosure of the present invention.

Claims (9)

1. a kind of preparation method of soluble dietary fiber, it is characterised in that comprise the following steps：

(1) pre-process：It will be beaten after fruit cleaning, attrition grinding adjusts pH and stirring prepares suspension；

(2) digest：Suspension into step (1) adds enzyme, adjusts pH and stirs, separation of solid and liquid, collects supernatant regulation pH To neutrality；

(3) UF membrane：The supernatant obtained to step (2) carries out hyperfiltration treatment, collects permeate；

(4) concentrate：The permeate concentration that step (3) is obtained；

(5) it is spray-dried：The concentrate that step (4) is obtained is spray-dried, and dried powder is soluble dietary Fiber.

2. preparation method according to claim 1, it is characterised in that step (1) described fruit be peach, apple, banana or In pineapple any one or at least two combination, preferably peach；

Preferably, the mashing described in step (1) is specifically included：The water of 1-10 times of fruit weight is added, is beaten using Mechanical Method Slurry processing, and fruit pulp and fruit core are separated；

Preferably, the attrition grinding described in step (1) is specifically included：The water of 1-30 times of fruit weight is added into fruit pulp, is adopted Fruit pulp is subjected to attrition grinding with Mechanical Method.

3. preparation method according to claim 1 or 2, it is characterised in that the pH described in step (1) is 3-11, is preferably 4-7；

Preferably, the temperature of the stirring described in step (1) is 20-80 DEG C, preferably 30-60 DEG C；

Preferably, the mixing time described in step (1) is 1-12h, preferably 3-10h.

4. the preparation method according to any one of claim 1-3, it is characterised in that step (2) enzyme and fruit Mass ratio is 1:(50-10000), preferably 1:(100-8000), more preferably 1:(500-6000)；

Preferably, the enzyme is pectinesterase, polygalacturonase, endoglucanase, exoglucanase or β-Portugal In polyglycoside enzyme any one or at least two combination.

5. the preparation method according to any one of claim 1-4, it is characterised in that the pH described in step (2) is 2-11, Preferably 3-8, more preferably 4-7；

Preferably, the temperature of the stirring described in step (2) is 10-80 DEG C, preferably 20-60 DEG C；

Preferably, the mixing time described in step (2) is 1-20h, preferably 3-16h.

6. the preparation method according to any one of claim 1-5, it is characterised in that the hyperfiltration treatment described in step (3) To carry out ultrafiltration using milipore filter；

Preferably, the molecular cut off of the milipore filter is 10-500kDa, more preferably preferably 30-400kDa, 50- 300kDa；

Preferably, the temperature of the ultrafiltration is 20-80 DEG C, preferably 30-60 DEG C.

7. the preparation method according to any one of claim 1-6, it is characterised in that the concentration described in step (4) is dense Solid content is reduced to for 5-25%, preferably 10-20%.

8. the preparation method according to any one of claim 1-7, it is characterised in that comprise the following steps：

(1) pre-process：After fruit cleaning, the water mashing of 1-10 times of fruit weight is added, the water of 1-30 times of fruit weight is added Attrition grinding, adjusts pH to 3-11,20-80 DEG C of stirring 1-12h, prepares suspension；

(2) digest：Suspension into step (1) is added and the mass ratio of fruit is 1:The enzyme of (50-10000), adjusting pH is 2-11,10-80 DEG C of stirring 1-20h, separation of solid and liquid collects supernatant and adjusts pH to neutrality；

(3) UF membrane：It is 10-500kDa milipore filter in 20- that the supernatant that is obtained to step (2), which use molecular cut off, Ultrafiltration is carried out at 80 DEG C, permeate is collected；

(4) concentrate：The permeate concentration that step (3) is obtained, is concentrated into solid content for 5-25%；

(5) it is spray-dried：The concentrate that step (4) is obtained is spray-dried, and dried powder is soluble dietary Fiber；

Wherein, step (1) described fruit is any one in peach, apple, banana or pineapple or at least two combination, step (2) enzyme is pectinesterase, polygalacturonase, endoglucanase, exoglucanase or beta-glucosidase In any one or at least two combination.

9. the preparation method according to any one of claim 1-8, it is characterised in that comprise the following steps：

(1) pre-process：After peach is cleaned, the water mashing of 2-8 times of fruit weight is added, the pigment for adding 5-25 times of fruit weight are broken Grinding, adjusts pH to 4-7,30-60 DEG C of stirring 3-10h, prepares suspension；

(2) digest：Suspension into step (1) is added and the mass ratio of fruit is 1:The enzyme of (100-8000), adjusting pH is 3-8,20-60 DEG C of stirring 3-16h, separation of solid and liquid collects supernatant and adjusts pH to neutrality；

(3) UF membrane：It is 30-400kDa milipore filter in 30- that the supernatant that is obtained to step (2), which use molecular cut off, Ultrafiltration is carried out at 60 DEG C, permeate is collected；

(4) concentrate：The permeate concentration that step (3) is obtained, is concentrated into solid content for 10-20%；

(5) it is spray-dried：The concentrate that step (4) is obtained is spray-dried, and dried powder is soluble dietary Fiber；

Wherein, step (2) described enzyme be pectinesterase, polygalacturonase, endoglucanase, exoglucanase or In beta-glucosidase any one or at least two combination.