CN104886110A - Multipurpose composite cationic surfactant type disinfectant - Google Patents

Abstract

The invention relates to a multipurpose compound cationic surfactant disinfectant, which is composed of other cationic surfactants, chlorhexidine acetate, ethanol and water. The composite disinfectant provided by the invention can quickly kill bacteria such as Escherichia coli, salmonella, streptococcus and staphylococcus aureus, and is widely used in fields such as animal husbandry.

Description

A multi-purpose compound cationic surfactant disinfectant

technical field

The invention relates to a new type of disinfectant, in particular to a compound veterinary disinfectant containing cationic surfactant which is suitable for disinfection of livestock and poultry, livestock houses and livestock, and disinfection of skin and mucous membranes. It is a new generation of multi-purpose veterinary disinfectant. agent.

Background technique

At present, chemical disinfectants are widely used in the production of livestock and poultry industry, mainly divided into surfactants, halogens (chlorine preparations, iodine preparations, bromine preparations), aldehydes, phenols, alcohols, oxidants, acids, etc. Alkalis, etc. All kinds of chemical disinfectants play an important role in preventive disinfection, treatment of diseases, improvement of environment and water quality, and each has its own advantages and disadvantages. For example: phenolic disinfectants (phenol, creol soap) have a killing effect on most microbial propagules, but have no effect on spores and viruses, and sterilization will destroy animal tissue cells; aldehyde disinfectants (formaldehyde, glutaraldehyde etc.), which have high-efficiency broad-spectrum bactericidal effect and can kill spores, but they are highly irritating to humans and animals and unstable in nature; chlorine-containing disinfectants (84-disinfectant, bleach, etc.) can kill bacteria, spores and Viruses can also bleach items, but the duration of the drug effect is short, the drug is not easy to store for a long time, and it is highly irritating and corrosive. It must be diluted before use. Long-term use will cause serious damage to the environment. An ideal disinfectant should have a series of characteristics such as broad bactericidal spectrum, fast action speed, good stability, low toxicity, low irritation, no pollution to the environment, and safe for humans and animals.

Surfactants are widely used in washing, sterilization, emulsification, cosmetics, printing and dyeing, oil extraction and other fields. Especially in recent years, the development speed is very fast, and the types and quantities of disinfectant-related products approved for marketing are constantly increasing. One of the cationic surfactants has outstanding performance, especially in terms of sterilization: it can be positively charged in water, adsorbed on the surface of microorganisms, form micelles, and gradually penetrate into the lipid layer and protein layer of the cytoplasm, thereby changing Permeability of the cell membrane, allowing extravasation of cell contents, leading to microbial death. At the same time, it coagulates proteins, denatures enzymes and structural proteins, destroys the metabolism of microorganisms, and kills microorganisms. However, among surfactants, although many traditional disinfectants have a strong bactericidal effect, they are not easy to decompose, have irritating properties, and poor stability. They are not suitable for long-term and large-scale use, and have great limitations.

Chlorhexidine Acetate, also known as Chlorhexidine Acetate, Chlorhexidine Acetate, Chlorhexamethylene Biguanide, Chlorhexidine, is a divalent cationic surfactant, which has obvious effect on bacteria Affinity, good sterilizing effect, and non-irritating, it is an ideal skin disinfectant, and it is also a broad-spectrum antibacterial agent, which has strong antibacterial effect on Gram-positive bacteria and Gram-negative bacteria , but there is a certain defect in using chlorhexidine acetate alone, because it is insoluble in water.

Studies have shown that the compound system of two or more surfactants can produce synergistic effects. Foreign countries have long started to study the related properties of surfactants, and there are related compound patent reports, such as:

Compounding of ethanol and chlorhexidine (EP0707794A1);

Hydrogen peroxide, lactic acid, chlorhexidine, lauryl sulfate 0.1-3% compound system (EP1027827A1);

There are also related patent reports in China:

A disinfectant composed of 0.1-0.3% single- and double-chain compound quaternary ammonium salts, 0.1-0.3% chlorhexidine acetate, etc. (CN200810102042.0 (publication number 101537000A));

A hand sanitizer (CN00111388.7 (publication number is CN1345536A)) composed of 0.3-0.8 g of chlorhexidine acetate and 0.05-0.1 g of dodecylmethylamine oxide.

Iodophor is an amorphous combination of iodine and surfactants, with little irritation and corrosion, and has been widely used in the disinfection of skin and tableware.

Although there are new products related to the above compounds in recent years, such compound products also have shortcomings, for example, the content of active ingredients is relatively high, the application is not particularly wide, and they cannot provide high-efficiency broad-spectrum disinfectants, etc. Therefore, it can be considered to use cationic surfactants with excellent performance to compound with other reagents to develop a new type of compound disinfectant. This method has become the focus of research at home and abroad.

Contents of the invention

The purpose of this invention is to provide a kind of multi-purpose composite cationic surfactant disinfectant.

A kind of multi-purpose composite cationic surfactant disinfectant provided by the invention is made up of chlorhexidine acetate, other cationic surfactants, ethanol and water.

Specifically, the compound cationic surfactant disinfectant is composed of the following components: 0.02-0.10 parts by weight of other cationic surfactants, 0.13-0.54 parts by weight of chlorhexidine acetate, 10-13 parts by volume of ethanol, and water to 100 parts by weight share.

Preferably, the compound cationic surfactant disinfectant consists of the following components: 0.02-0.07 parts by weight of other cationic surfactants, 0.13-0.18 parts by weight of chlorhexidine acetate, 10-13 parts by volume of ethanol, and water to make up to 100 parts by weight share.

Preferably, the compound cationic surfactant disinfectant consists of the following components: 0.07 parts by weight of other cationic surfactants, 0.13 parts by weight of chlorhexidine acetate, 10 parts by volume of ethanol, and 100 parts by weight of water.

In the above-mentioned composite cationic surfactant disinfectant:

The unit of said weight part corresponds to the unit of volume part, that is, when the unit of weight is kg, the volume part is L, the weight unit is g, and the volume part is ml.

The other cationic surfactants are chloride-N-dodecylpyridine-1-acetamide;

The ethanol is 15%.

The present invention also provides the preparation method of above-mentioned compound cationic surfactant disinfectant, and this method comprises the following steps:

1) At room temperature, add chlorhexidine acetate into ethanol and stir until completely dissolved, then add sterilized distilled water and let stand;

2) Weigh the cationic surfactant and add sterilized distilled water to mix and set aside;

3) Mix the mixed solution of chlorhexidine acetate and ethanol prepared above with the cationic surfactant solution.

Preferably, the method comprises the steps of:

1) At room temperature, add 2g of chlorhexidine acetate into 150mL of ethanol and stir until completely dissolved, then add sterilized distilled water to 1L, and let stand for 10min;

2) Weigh 1 g of cationic surfactant, add 500 mL of sterilized distilled water and mix well, set aside;

3) Mix the mixture of chlorhexidine acetate and ethanol prepared above with the cationic surfactant solution in a ratio of 2:1, and store at room temperature for later use.

The present invention also provides the application of the above-mentioned composite disinfectant in disinfection, and the scope of application mainly includes the disinfection of skin, wounds and mucous membranes, grounds of livestock houses, surfaces of general objects, etc.

When the compound disinfectant is in use, it should be diluted with sterile distilled water so that the final concentration of the cationic surfactant is about 0.004%, and it can be stored at room temperature.

Detailed ways

The advantage of compound cationic surfactant disinfectant provided by the invention:

1, the present invention integrates the advantages and disadvantages of various similar products at home and abroad, overcomes the deficiencies in the prior art, and adopts a novel high-efficiency cationic surfactant-chlorinated-N-dodecylpyridine-1-acetamide (English is sodium dodecyl chloride-AND-pyridine-1-acetamide) compounded with chlorhexidine acetate and synergist to provide a compound veterinary compound veterinary product with a wide spectrum of sterilization and high efficiency, which is suitable for disinfection of livestock and poultry, livestock houses and livestock disinfectant.

2. Compared with commercially available disinfectants, the combination of the present invention has lower concentration, lower price, and outstanding bactericidal effect, especially for Gram-positive bacteria, and has a wide range of uses.

Detailed ways

The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

Embodiment 1: composite cationic surfactant disinfectant

1. Composition (1L):

compound ingredients Content (g/L) Final concentration(%) 1 Chloro-N-dodecylpyridine-1-acetamide 0.67 (g) 0.07 2 Chlorhexidine acetate 1.33 (g) 0.13 3 Ethanol (15% concentration) 100(mL) 10 4 water Add water to 1L

2. Preparation method:

1) At room temperature, add 2g of chlorhexidine acetate into 150mL of ethanol and stir until completely dissolved, then add sterilized distilled water to 1L, and let stand for 10min;

2) Weigh 1 g of chlorinated-N-dodecylpyridine-1-acetamide, add 500 mL of sterilized distilled water and mix to obtain a cationic surfactant solution, which is set aside;

3) The mixed solution of chlorhexidine acetate and ethanol prepared above is mixed with the cationic surfactant solution, that is, the mixed solution of 2L chlorhexidine acetate and ethanol is added to 1L cationic surfactant solution, stirred evenly, and left to stand to obtain a compound Disinfectant stock solution (the concentration of chlorhexidine acetate is 0.13%, the concentration of cationic surfactant is 0.07%, and the concentration of ethanol is 10%);

3. How to use:

Dilute the stock solution 16 times with sterile distilled water (the final concentration of chloride-N-dodecylpyridine-1-acetamide is about 0.004%), perform ordinary disinfection for 1-2 minutes, and sterilize for 10 minutes.

Embodiment 2: composite cationic surfactant disinfectant

1. Composition (1L):

compound ingredients Content (g/L) Final concentration(%) 1 Chloro-N-dodecylpyridine-1-acetamide 0.4(g) 0.04 2 Chlorhexidine acetate 1.6(g) 0.16 3 Ethanol (15% concentration) 120(mL) 12 4 water Add water to 1L

2. Preparation method:

1) At room temperature, add 2g of chlorhexidine acetate into 150mL of ethanol and stir until completely dissolved, then add sterilized distilled water to 1L, and let stand for 10min;

2) Weigh 1 g of chlorinated-N-dodecylpyridine-1-acetamide and add 500 mL of sterilized distilled water to mix evenly to prepare a cationic surfactant solution, which is set aside;

3) The mixed solution of chlorhexidine acetate and ethanol prepared above is mixed with the cationic surfactant solution, that is, the mixed solution of 4L chlorhexidine acetate and ethanol is added to 1L cationic surfactant solution, stirred evenly, and left to stand to obtain a compound Disinfectant stock solution (the concentration of chlorhexidine acetate is 0.16%, the concentration of cationic surfactant is 0.04%, and the concentration of ethanol is 12%);

3. How to use:

Dilute the stock solution 16 times with sterile distilled water (the final concentration of chloride-N-dodecylpyridine-1-acetamide is about 0.003%), perform normal disinfection for 1-2 minutes, and sterilize for 10 minutes.

Embodiment 3: composite cationic surfactant disinfectant

1. Composition (1L):

compound ingredients Content (g/L) Final concentration(%) 1 Chloro-N-dodecylpyridine-1-acetamide 0.2(g) 0.02 2 Chlorhexidine acetate 1.6(g) 0.16 3 Ethanol (15% concentration) 130(mL) 13 4 water Add water to 1L

2. Preparation method:

1) At room temperature, add 2 g of chlorhexidine acetate into 150 mL of ethanol (15% ethanol concentration) and stir until completely dissolved, then add sterilized distilled water to 1 L, and let stand for 10 min;

2) Weigh 1 g of chlorinated-N-dodecylpyridine-1-acetamide, add 500 mL of sterilized distilled water and mix to obtain a cationic surfactant solution, which is set aside;

3) The mixed solution of chlorhexidine acetate and ethanol prepared above is mixed with the cationic surfactant solution, that is, the mixed solution of 8L chlorhexidine acetate and ethanol is added to 1L cationic surfactant solution, stirred evenly, and left to stand to obtain a compound Disinfectant stock solution (the concentration of chlorhexidine acetate is 0.16%, the concentration of cationic surfactant is 0.02%, and the concentration of ethanol is 13%);

3. How to use:

Dilute the stock solution 16 times with sterile distilled water (the final concentration of chloride-N-dodecylpyridine-1-acetamide is about 0.001%), perform normal disinfection for 1-2 minutes, and sterilize for 10 minutes.

Experimental example 1: Disinfection effect detection test

1. Test method

The test was carried out with reference to the national standard (GB15981-1995) method, and the test bacteria included Escherichia coli, Salmonella enteriditis, Streptococcus, and Staphylococcus aureus. The specific test steps are as follows:

1. Determination of 10min minimum inhibitory concentration

1.1 Test method: Refer to the national standard (GB15981-1995) method, use PBS to prepare indicator bacteria into a bacterial suspension of 5×10 ⁵ -5×10 ⁶ CFU/mL. Use 0.2% cationic surfactant (chloride-N-dodecylpyridine-1-acetamide) solution and 0.2% chlorhexidine acetate solution in the ratio of 1:2, 1:4, 1:8 respectively Mixed, and each group was diluted with sterilized distilled water to the same concentration of monomer control. Then the three compound preparations and the control monomer were serially diluted to 5 different concentrations by 2 times with sterilized distilled water respectively, each pipetting 4.5mL was added into the test tube, and then 0.5mL bacterial suspension was added to the test tube respectively, and put in 20± In a water bath at 2°C, mix well and start counting the time. After 10 minutes, take 0.5m L of the bacterial liquid mixture, make appropriate dilutions, count the viable bacteria, and calculate the killing rate.

1.2 Calculation of killing rate

Killing rate=(the number of bacteria in the control group-the number of bacteria in the sample group)/the number of bacteria in the control group×100%.

2. Qualitative disinfection test

2.1 Test method: Refer to the qualitative disinfection test in the national standard (GB15981-1995) method, prepare the bacterial suspension of 5×10 ⁵ -5×10 ⁶ CFU/mL from the bacterial isolate. Select the concentration of the disinfectant according to the results of the 10min minimum antibacterial test, serially dilute it with 2 times of sterilized distilled water, and set a test tube without disinfectant as a control. Add an equal amount of 2.5mL of bacterial suspension into the test tube, take out 0.5mL at 1min, 5min, 10min, and 15min after the addition of bacteria at four different intervals, and add 4.5mL of neutralizing agent (neutralizing agent is containing 1% lecithin, 1% sodium thiosulfate, 1.5% Tween-80 in PBS), after neutralizing for 10 minutes, take out 0.5mL and add it into a 4.5mL nutrient broth tube. Place the broth tube inoculated with bacteria at 37°C for 48 hours, and observe the preliminary results until the 7th day. The test was repeated 5 times, and the results were judged.

2.2 Results Judgment Criteria:

① If the broth tube is turbid, it means that there is bacterial growth, and it is recorded as positive, indicated by (+);

②If the broth tube is clear after culturing until the 7th day, it means that the aseptic growth is recorded as negative, indicated by (-);

③ For broth tubes that are difficult to determine, take 10 mL and inoculate them on nutrient agar plates, spread evenly with a sterilized L stick, and incubate at 37°C for 48 hours to observe the colony shape; and smear staining and microscopic examination to determine whether there are indicator bacteria growing. The growth of indicator bacteria was recorded as positive;

④ Repeat the test 5 times, if no indicator bacteria grow, it means that the sterilization has been achieved.

3. Quantitative disinfection test:

3.1 Test method: Refer to the quantitative disinfection test in the national standard (GB15981-1995) method to prepare a bacterial suspension of 5×10 ⁵ -5×10 ⁶ CFU/mL. Select the concentration of this veterinary disinfectant according to the 10min minimum antibacterial test, dilute the disinfectant 2 times serially with sterilized distilled water, draw 4.5mL into the test tube, and put it in a water bath at 20±2°C. After the temperature of the liquid in the test tube is balanced with the temperature of the water bath, add 0.5 mL of bacterial suspension into the test tube, mix well and start timing. At 1min, 5min, 10min, and 15min, respectively, at four different intervals, 0.5mL of the bacterial liquid mixture was transferred into 4.5mL of the neutralizer and mixed evenly. Neutralize for 10 minutes, count viable bacteria after appropriate dilution, and calculate the killing rate.

3.2 Calculation of kill rate

Killing rate=(the number of bacteria in the control group-the number of bacteria in the sample group)/the number of bacteria in the control group×100%.

4. Organic matter protection test

4.1 Test method: Refer to the organic matter protection test in the national standard (GB15981-1995) method, count the viable bacteria in the bacterial solution, dilute it with a diluent, add calf serum, so that the final serum content is 10%, and the number of bacteria is 5×10 ⁵ -5×10 ⁶ CFU/mL, as the test bacteria suspension.

Select a disinfectant with a compound ratio of 1:2 to determine the bactericidal effect in the presence of organic matter according to the aforementioned method.

4.2 Results Judgment Criteria:

①The killing rate of 5 tests is greater than 99.9% of the required minimum concentration and shortest time, it is judged that the disinfectant can reach the effective concentration and time of disinfection in the presence of organic matter;

②The effective concentration and time are the same or close to the effective concentration and time of the quantitative disinfection test to achieve disinfection, and it is judged that the organic matter has no obvious effect on the bactericidal effect of the disinfectant.

Those who achieve the minimum effective concentration of disinfection by more than double or the shortest action time by more than double can be considered to have obvious effects.

5. On-site sterilization test

Test method: On-site disinfection effect test in cattle farm According to the 2001 edition of "Compilation of Technical Specifications for Veterinary Drug Testing", select a disinfectant with a compound ratio of 1:2 to conduct disinfection tests on the air, ground, and skin of the cattle house, and pass the colony counting method Calculate the sterilization rate to evaluate the disinfection effect on the spot, the specific steps are as follows:

(1) Sterilization and disinfection test of cowshed air

Field Disinfection Test in Cattle Farm The test was carried out in the finishing barn of a farm in Heilongjiang Province. Clean the barn before sterilization, and seal the windows and ventilation holes tightly. Because the field test season is in October, the temperature inside the barn is around 8-15°C. Dilute the disinfectant solution with distilled water into three different concentrations (1:8, 1:16, 1:32) for the test, use a sprayer to spray the air in the house, and use the plate sedimentation method to test before disinfection, 10 minutes after disinfection, and 10 minutes after disinfection. Sampling once every 20min, 40min, and 60min, and opening the lid of the petri dish for 10min after each sampling, and then cover it well. The collected samples were cultured in a 37°C incubator for 24 hours, and finally the colony counting method was used to count the cultures of each sample.

(2) Sterilization and disinfection test on the floor of the barn

The disinfectant solution was diluted with distilled water to prepare three concentrations (1:8, 1:16, and 1:32) of the disinfectant solution. Select 3 areas in the cowshed, each with an area of 1×1m ² , remove feces and dirt on the ground, and disinfect the 3 areas with diluted disinfectants of different concentrations. In the above 3 sampling areas, set 4 sampling points along the diagonal, each sampling point has an area of 10×10cm ² , and sample once before disinfection, 10min, 20min, 40min, and 60min after disinfection, and each sampling Dip the aqueous solution containing the neutralizing agent with a sterilized cotton swab, roll and rub it on the sampling point, and then put it into a test tube containing the aqueous neutralizing agent (5ml/tube). Take the sample back to the laboratory, use the method of pulling and blowing to fully wash off the bacteria on the cotton swab, dilute the bacterial suspension 10 times with sterilized saline, and incubate the collected sample in a 37°C incubator for 24 hours. Culture counts were performed on each sample.

(3) Sterilization test of bovine skin

The cow's mammary gland was selected for the test, and disinfected with three concentrations of disinfectants (1:8, 1:16, and 1:32, respectively). Samples were taken at the sterilized site before disinfection, 10 min, 20 min, 40 min, and 60 min after disinfection, and the samples were incubated in a 37°C incubator for 24 hours, and each sample was cultured and counted.

2. Test results

1. 10min minimum inhibitory concentration test results

1.1 The minimum inhibitory concentration test results for Escherichia coli: see Table 1

Table 1 The minimum inhibitory concentration determination results for Escherichia coli

Note: The initial bacterial count is 5×10 ⁶ /mL (a): 1:2 compound ratio; (b): 1:4 compound ratio; (c): 1:8 compound ratio

1.2 The minimum inhibitory concentration test results for Salmonella: see Table 2

Table 2 The minimum inhibitory concentration determination results for Salmonella

Note: The initial bacterial count is 5×10 ⁶ /mL (a): 1:2 compound ratio; (b): 1:4 compound ratio; (c): 1:8 compound ratio

1.3 The minimum inhibitory concentration test results for streptococci: see Table 3

Table 3 Determination results of the minimum inhibitory concentration for streptococci

Note: The initial bacterial count is 5×10 ⁶ /mL (a): 1:2 compound ratio; (b): 1:4 compound ratio; (c): 1:8 compound ratio

1.4 The minimum inhibitory concentration test results for Staphylococcus aureus: see Table 4

Table 4 Determination results of minimum inhibitory concentration for Staphylococcus aureus

Note: The initial bacterial count is 5×10 ⁶ /mL (a): 1:2 compound ratio; (b): 1:4 compound ratio; (c): 1:8 compound ratio

The results of Tables 1-4 show that:

When the compounding ratio of cationic surfactant and chlorhexidine acetate is 1:2, the minimum dilution factor for 10min sterilization of Escherichia coli and Salmonella is 1:16 (the cationic surfactant and chlorhexidine acetate disinfect The minimum inhibitory concentration of the compound fungicide is 0.004% and 0.008% respectively when the compound ratio is 1:2). When the dilution factor is 1:256, the bactericidal rate of the compound fungicide is still 100%.

The results showed that the bactericidal effect of the control group was not as good as that of the compound disinfectant, and the bactericidal effect of the compound disinfectant on Gram-positive bacteria was very prominent.

2. Qualitative disinfection test results for different bacteria: see Table 5

Table 5 Qualitative disinfection test results for different bacteria

Note: The initial bacterial count was 5x10 ⁶ /mL and the control group was +

The results in Table 5 show that: according to the above 10min minimum antibacterial test results, the compound new disinfectant stock solution with a compound ratio of 1:2 is selected for appropriate dilution, and then the qualitative disinfection test of the disinfectant is carried out. For Escherichia coli and Salmonella, use this disinfectant to dilute 1:16 times to meet the sterilization requirements within 10 minutes; for streptococcus and Staphylococcus aureus, it is better to use this disinfectant to dilute 1:512 times in 1 minute. Meet the sterilization requirements.

3. Quantitative disinfection test results

Table 6 The results of the quantitative disinfection test for different bacteria

Note: The number of initial bacteria and the number of bacteria in the control group is 5*10 ⁶ /mL

The results in Table 6 show that for Escherichia coli and Salmonella, this disinfectant is diluted 1:16 times and reaches the disinfection requirements in 1min; the killing effect of streptococcus and Staphylococcus aureus is better, and this disinfectant is diluted 1:512 times, and it can be disinfected in 1min. It can meet the disinfection requirements, and the sterilization rate is above 99.9%.

4. Organic matter protection test results: see Table 7

Table 7 The results of the protection test for different bacterial organisms

Note: The number of initial bacteria and the number of bacteria in the control group is 5*10 ⁶ /mL

The results in Table 7 show that the veterinary disinfectant, under the action of organic matter, meets the disinfection requirements for Escherichia coli and Salmonella at a 1:16 dilution in 1 minute; The 1:512 dilution of the disinfectant can meet the disinfection requirements in 1 minute, and the bactericidal rate is over 99.99%, which is similar to the quantitative disinfection test results of the disinfectant. There was no significant effect on the bactericidal effect of disinfectants.

5. On-site application implementation cases

On-site test According to the above test results, a disinfectant with a compound ratio of 1:2 was selected for on-site disinfection verification test. The details are as follows:

5.1 Results of the air disinfection test on the barn: see Table 8

Table 8 Results of the air disinfection test on the barn

Note: The number of bacteria before disinfection is 1.8*10 ⁵ /mL

Table 8 result shows, cationic surfactant and chlorhexidine acetate compound disinfectant, in 10min action time, the killing rate can reach 99.9% under the concentration of 1:8 times dilution (cationic surfactant and chlorhexidine acetate Disinfectant concentrations are 0.009% and 0.02% in turn); the other two concentrations have not reached this killing rate, but are very close; they can all reach the judgment standard when the time is 20min, 40min, and 60min; monomeric disinfectants have not reached the standard.

5.2 The results of the disinfection test on the ground of the cowshed: see Table 9

Table 9 The results of the disinfection test on the ground of the cowshed

Note: The number of bacteria before disinfection is 2.9*10 ⁵ /mL

Table 9 result shows, cationic surfactant and chlorhexidine acetate compound disinfectant, in 10min action time, the killing rate under the concentration of 1:8 times of dilution is 99.92% (cationic surfactant and chlorhexidine acetate disinfection Concentration of disinfectant is 0.009%, 0.02% successively), other concentrations can not reach the standard of killing rate ≥ 99.9%; But it can be done under the time of 20min, 40min, 60min; Monomer disinfectants have not reached the judgment standard.

5.3 Results of disinfection test on bovine skin: see Table 10

Table 10 Results of disinfection test on bovine body skin

Note: The number of bacteria before disinfection is 1.1x10 ⁵ /mL

Table 10 result shows, cationic surfactant and chlorhexidine acetate compound disinfectant can reach ≥ 99.9% judgment standard under the concentration of 1:32 times of dilution under 10min action time (cationic surfactant and chlorhexidine acetate The fixed disinfectant concentration is 0.002%, 0.004% in turn), and when the time is 20min, 40min, 60min, it has better bactericidal effect; the monomeric disinfectant has not reached the judgment standard.

Based on the above results, it is shown that cationic surfactant (chlorination-N-dodecylpyridine-1-acetamide) and chlorhexidine acetate disinfectant are used in combination according to the ratio of 1:2, which can produce synergistic effect The role can expand the bactericidal spectrum. Simultaneously, the bactericidal ability of the chlorhexidine acetate has not only been strengthened, but also the use concentration of the cationic surfactant (chlorinated-N-dodecylpyridine-1-acetamide) has been reduced, and the cost has been reduced.

Although, the present invention has been described in detail with general description, specific implementation and test above, but on the basis of the present invention, some modifications or improvements can be made to it, which will be obvious to those skilled in the art . Therefore, the modifications or improvements made on the basis of not departing from the spirit of the present invention all belong to the protection scope of the present invention.

Claims (10)

1. a multiduty composite cation surfactant disinfectants, it is made up of chlorhexidine acetate, other cationic surfactants, second alcohol and water.

2. composite cation surfactant disinfectants according to claim 1, it is characterized in that, described composite disinfectant consists of the following composition: other cationic surfactants 0.02-0.10 weight portion, chlorhexidine acetate 0.13-0.54 weight portion, ethanol 10-13 parts by volume, water complements to 100 weight portions.

3. composite cation surfactant disinfectants according to claim 2, it is characterized in that, described composite disinfectant consists of the following composition: other cationic surfactants 0.02-0.07 weight portion, chlorhexidine acetate 0.13-0.18 weight portion, ethanol 10-13 parts by volume, water complements to 100 weight portions.

4. composite cation surfactant disinfectants according to claim 3, it is characterized in that, described composite disinfectant consists of the following composition: other cationic surfactant 0.07 weight portions, chlorhexidine acetate 0.13 weight portion, ethanol 10 parts by volume, water complements to 100 weight portions.

5. the composite cation surfactant disinfectants according to any one of claim 1-4, is characterized in that, other cationic surfactants described are chlorination-N-dococylpyridinium-1-acetamide.

6. the composite cation surfactant disinfectants according to any one of claim 1-4, is characterized in that, the concentration of described ethanol is 15%.

7. prepare a method for the composite cation surfactant disinfectants described in any one of claim 1-6, it is characterized in that, the method comprises the following steps:

1) at room temperature, chlorhexidine acetate is added in ethanol and stir to dissolving completely, add sterile purified water afterwards, leave standstill;

2) take cationic surfactant and add sterile purified water mixing, for subsequent use;

3) chlorhexidine acetate of above-mentioned preparation and the mixed liquor of ethanol are mixed with cationic surfactant solution.

8. method according to claim 7, is characterized in that, the method comprises the following steps:

1) at room temperature, 2g chlorhexidine acetate is added in 150mL ethanol and stir to dissolving completely, add sterile purified water afterwards to 1L, leave standstill 10min;

2) take cationic surfactant 1g and add the mixing of 500mL sterile purified water, for subsequent use;

3) chlorhexidine acetate of above-mentioned preparation and the mixed liquor of ethanol are mixed with the ratio of cationic surfactant solution according to 2:1, room temperature preservation is stand-by.

9. the application of composite cation surfactant disinfectants in sterilization described in any one of claim 1-6.

10. application according to claim 9, is characterized in that, described application comprises the sterilization of skin, the surface of a wound and mucous membrane, animal house ground or general body surface.