CN104013560A - Traditional Chinese medicine composition with whitening effect and application thereof in cosmetics - Google Patents

Abstract

The invention discloses a traditional Chinese medicine composition with a whitening effect. The traditional Chinese medicine composition is characterized by consisting of the following raw materials: 30-50 percent of Chinese angelica, 25-35 percent of rhizoma bletillae, 10-15 percent of Lysimachia christinae Hance, 10-15 percent of sage and 5-10 percent of liquorice. The preparation method comprises the following steps: grinding the traditional Chinese medicine composition, screening, adding an extraction solvent according to a liquid material mass ratio of 10:1-30:1, soaking for 1-2 hours, performing normal temperature extraction under the pressure of 50-100MPa by using a high-pressure homogenizing extractor, performing centrifugal separation on the extracting solution, adsorbing by using a macroporous resin, repeatedly eluting by using 10-95 percent of ethanol, collecting the eluent, performing reduced pressure concentration until the mass ratio of the extracting solution to the traditional Chinese medicine is 1:0.5-1:10; and adding butanediol with the same mass as the extracting concentrate, filtering and preparing the extract of the traditional Chinese medicine composition. The extract of the traditional Chinese medicine composition prepared by the method has the effects of strongly inhibiting melanogenesis, eliminating free radicals and realizing sun protection, is used in cosmetics and achieves the effect of whitening the skin.

Description

A whitening traditional Chinese medicine composition and its application in cosmetics

the

technical field

The invention relates to a traditional Chinese medicine composition, in particular to the application of a whitening traditional Chinese medicine composition and its extract in cosmetics.

Background technique

Since ancient times, whiteness has been regarded as beauty in China. As the saying goes, "one white covers all ugliness", fair skin is the lifelong pursuit of all women. In addition to natural dark skin, from the perspective of traditional Chinese medicine, not white is often a manifestation of poor health, imbalance of viscera and blood, and a sub-healthy state, often showing symptoms such as chloasma and freckles on the face. For the treatment of facial spots, there are methods such as laser or intense pulsed light therapy, external drug therapy, etc.; wherein laser or intense pulsed light therapy is very harmful to the skin and is not commonly used; cosmetic whitening is the most commonly used method at present.

There are major defects in the management and technology of whitening cosmetics in my country. The phenomenon of illegal addition is very serious. Some even use contraband such as mercuric chloride as whitening agent or add peroxide such as hydrogen peroxide for bleaching. Over the years, many beauty products have been caused. Accidents, even disfigurement, have caused great harm to consumers, and have attracted great attention from the country and consumers. In recent years, the whitening agents used in cosmetics mainly include compounds such as hydroquinone (hydroquinone), L-ascorbic acid (vitamin C), kojic acid, superoxide dismutase (SOD) and arbutin. But these whitening agents have many defects, such as the irritation of hydroquinone is very large, vitamin C and SOD are very unstable, kojic acid also has certain irritation and easy discoloration, and arbutin has a single effect and poor effect. Therefore, the development of natural whitening agents with good safety, stability, obvious inhibitory effect and good cost performance has become one of the main research directions of the current pharmaceutical and cosmetic industries, and has very good development prospects.

Traditional Chinese medicine whitening is one of the important contents of traditional Chinese medicine beauty treatment, and it is also an important part of Chinese medicine. In traditional Chinese medicine whitening, it pays attention to slow adjustment and careful treatment, mainly to treat the root cause, and the curative effect is relatively stable. This is a very traditional, but long-lasting, safe and effective method.

Contents of the invention

The object of the present invention is to provide a natural and safe whitening Chinese medicine composition and a preparation process of the extract of the Chinese medicine composition.

For achieving the purpose of the present invention, the present invention adopts following technical scheme:

A traditional Chinese medicine composition for whitening, which is characterized in that it comprises the following components in weight percentage: 20-50% of Angelica sinensis, 10-30% of Baiji, 6-18% of Desmodium, 5-20% of Sage, 5-15% of Licorice %.

Preferably, the whitening traditional Chinese medicine composition ( I ) by weight is: 38% Angelica sinensis, 24% Baiji, 14% Desmodium, 10% Sage, and 14% Licorice.

Preferably, the whitening traditional Chinese medicine composition ( II ) by weight is: 40% Angelica sinensis, 28% Rhizoma baicalensis, 12% Desmodium, 10% Sage, and 10% Licorice.

Preferably, the whitening traditional Chinese medicine composition ( Ⅲ ) by weight is: 35% Angelica sinensis, 25% Radix Sinensis, 15% Desmodium, 13% Sage, and 12% Licorice.

The preparation process of the extract of the above-mentioned whitening Chinese medicine composition is characterized in that: take the Chinese medicine raw materials Angelica sinensis, Radix Sinensis, Desmodium, Clary Sage, and Glycyrrhizae according to the proportioning, pulverize the above-mentioned Chinese medicine composition, sieve, and weigh according to the quality of the liquid material. Ratio of 10:1 to 30:1 was added to the extraction solvent, soaked for 1 to 2 hours, and extracted at room temperature with a high-pressure homogeneous extractor under a pressure of 50 to 100 MPa. After the extract was centrifuged, it was adsorbed with a macroporous resin. ~95% ethanol was eluted multiple times, the eluate was collected, concentrated under reduced pressure until the mass ratio of the extract to the traditional Chinese medicine was 1:0.5 ~1:10, then added butanediol of the same quality as the extract concentrate to dissolve, and filtered , to prepare the extract of the traditional Chinese medicine composition. In order to avoid solvent residue, the extraction solvent used in the present invention can adopt water, ethanol, ethanol aqueous solution.

The second object of the present invention is to use the extract of the whitening traditional Chinese medicine composition in cosmetics to produce whitening cosmetics with stable properties and good effects.

In order to achieve the second purpose of the present invention, the present invention adopts the following technical scheme: the extract of the above-mentioned Chinese medicine composition is used to prepare cosmetics, and it is characterized in that the extract of the above-mentioned Chinese medicine composition is contained in the cosmetics, and its consumption is 0.01 ~ 20 %, the preferred dosage is 1-10%.

The beneficial effects of the present invention are: the present invention is based on the theory of traditional Chinese medicine, and carries out compound prescription according to the concept of monarch, minister, assistant and envoy. In the present invention, angelica is the monarch drug, which is sweet and warm in nature and flavor, returns to the liver, heart, and spleen meridians, greatly invigorates vitality, contains a large amount of volatile oil, flavonoids, ferulic acid and other powerful whitening and blood-activating ingredients, and has very good The beauty effect of nourishing the skin, whitening and removing freckles. Baiji is the essence of ministers, its nature is bitter, sweet, astringent, slightly cold, and it belongs to the lung, liver, and stomach meridians. It is rich in amino acids, polysaccharides, and cinnamic acid and other whitening and moisturizing compounds. The power of promoting muscle growth, "Compendium of Materia Medica" says that it "cleanes the face black and removes freckles". Desmodium and clary sage are used as adjuvant medicine. Desmodium is sweet , slightly bitter , cool in nature, and belongs to the liver , gallbladder , kidney , and bladder meridian. It is rich in quercetin and other flavonoids that have strong anti-free radical and whitening effects Compound, has the functions of clearing away heat and diuresis, expelling wind and relieving pain, hemostasis and promoting muscle growth, anti-inflammatory and detoxification; sage is bitter, pungent, and flat in nature, promotes blood circulation and dispels blood stasis, dispels wind and dampness, detoxifies and eliminates accumulation; licorice is used to reconcile various medicines , sweet, flat, into the spleen, stomach, lung meridians, rich in glycyrrhetinic acid, licorice flavonoids and other powerful whitening and anti-free radical ingredients, have the power of nourishing the spleen and Qi, clearing away heat and detoxification, whitening and removing spots. The combination of the above-mentioned medicines can synergize, nourish qi and generate blood, nourish the skin, strengthen the body and brighten the complexion. The extract of the traditional Chinese medicine composition of the present invention can effectively scavenge free radicals, inhibit tyrosinase activity, resist ultraviolet rays, reduce melanin production, accelerate the renewal of skin surface cells, and delay skin aging. When added to cosmetics, the effect of whitening and brightening is remarkable. .

Description of drawings

Accompanying drawing 1 is the comparison diagram of skin melanin content using whitening skin care milk and common skin care milk.

Detailed ways

The present invention will be further described below through specific examples, but these examples are only for illustration, not limitation of the present invention.

Example 1 Extract (I) and preparation method of traditional Chinese medicine composition

Take by weighing 38g of Chinese medicine raw materials Angelica sinensis, 24g of Baiji, 14g of desmodium, 10g of sage, and 14g of licorice, pulverize the above-mentioned Chinese medicine composition, sieve, and add a volume fraction of 50% according to the liquid-material mass ratio of 15:1 After soaking for 2 hours, use a high-pressure homogeneous extractor to extract at room temperature under a pressure of 80 MPa. After the extract is centrifuged, it is adsorbed with AB-8 macroporous resin, and the volume fraction is 30%, 60% , 90% ethanol for multiple elutions, collect the eluate, concentrate under reduced pressure to 50 g of the extract, then add 50 g of butanediol to dissolve, filter, and obtain the extract of 100 g of the Chinese medicine composition.

Example 2 Extract (II) and preparation method of traditional Chinese medicine composition

Take by weighing 40g of Chinese medicine raw materials Angelica sinensis, 28g of Baiji, 12g of desmodium, 10g of sage, and 10g of licorice, pulverize the above-mentioned Chinese medicine composition, sieve, and add a volume fraction of 60% according to the liquid-material mass ratio of 18:1 After soaking for 1.5 hours, use a high-pressure homogeneous extractor to extract at room temperature under a pressure of 75 MPa. After the extract is centrifuged, it is adsorbed with D101 macroporous resin, and the volume fractions are 20%, 50%, and 95% ethanol was eluted multiple times, the eluate was collected, concentrated under reduced pressure to 50 g of extract, then 50 g of butanediol was added to dissolve, filtered, and 100 g of the extract of the traditional Chinese medicine composition was obtained.

Comparative example 1-5 Preparation of single Chinese medicine extract

Weigh 100g of Chinese medicine raw materials Angelica sinensis, 100g of Baiji, 100g of Desmodium, 100g of Sage, and 100g of Licorice, respectively pulverize the above-mentioned Chinese medicines, sieve, add ethanol with a volume fraction of 50% according to the mass ratio of liquid to material of 15:1 Extract the solvent, after soaking for 2 hours, extract at room temperature with a high-pressure homogeneous extractor under the pressure of 80MPa. % ethanol was eluted multiple times, the eluate was collected, concentrated under reduced pressure to 50g of the extract, then 50g of butanediol was added to dissolve, filtered, and 100g of Angelica sinensis extract, Baiji extract, Desmodium extract, Sage tail extract were obtained. Grass Extract, Licorice Extract.

Example 3 Preparation of whitening skin care milk

Table 1 shows the formula of whitening skin care milk containing the traditional Chinese medicine extract composition, and the consumption of each substance is represented by mass fraction.

Table 1

The preparation method of whitening skin care milk: cyclomethicone, cyclopentasiloxane, isopropyl palmitate, glyceryl caprylate, monoglyceride, stearic acid in the formula table , methylparaben, and propylparaben, heated to 80 ° C, melted and stirred evenly, and used as the oil phase; 1,2-propanediol, allantoin, Chinese medicine extract composition, triethanolamine in the formula table , deionized water, heated to 85 ° C, stirred and dissolved evenly, as the water phase. Add the oil phase and the water phase to the emulsification pot, stir evenly, emulsify and homogenize for 6 minutes, stir and cool to 50 ℃, add essence, stir well, cool to 38 ℃, it is whitening skin care milk.

Comparative Example 6 Preparation of ordinary skin care milk

Table 2 shows the formula of ordinary skin care milk that does not contain the traditional Chinese medicine extract composition, and the consumption of each substance is represented by mass fraction.

Table 2

The preparation method of ordinary skin care milk: cyclomethicone, cyclopentasiloxane, isopropyl palmitate, glyceryl caprylate, monoglyceride, stearic acid in the formula table , methylparaben, and propylparaben, heated to 80°C, melted and stirred evenly, and used as the oil phase; mixed 1,2-propanediol, allantoin, triethanolamine, and deionized water in the formula table, Heat to 85°C, stir to dissolve evenly, and use it as the water phase. Add the oil phase and the water phase to the emulsification pot, stir evenly, emulsify and homogenize for 6 minutes, stir and cool to 50°C, add essence, stir well, cool to 38°C, it is ordinary skin care milk.

the

Example 4 Preparation of whitening sleeping mask

Table 3 provides the whitening sleep mask formula containing the extract of the Chinese medicine composition of the present invention.

table 3

Its preparation method:

1. Stir and dissolve the oil phase raw materials in advance until they are uniformly dispersed, heat and dissolve the additional phase raw materials methylparaben and propylparaben in 1.3-butanediol until clear and transparent, and set aside.

2. Put the water phase raw materials into deionized water, heat to 75~85°C, stir until dissolved evenly.

3. Slowly and uniformly add the oil phase into the water phase under stirring conditions (1000~2000rpm). After the addition is complete, emulsify at a homogenization speed of 4000~6000rpm for 5~10 minutes.

4. Cool down to 40°C, add the active part extract and essence of traditional Chinese medicine, and then add aminomethyl propanol dropwise to adjust the pH value to 5.5~6.5.

5. Stir evenly, cool down to 35°C, retest the pH, vacuum defoam, filter and discharge.

the

Experimental example 1 In vitro evaluation experiment of extract whitening function

1. Sample preparation

The extract of the Chinese medicine composition prepared in Example 1 and the single Chinese medicine extract prepared in Comparative Examples 1-5 were used as samples. Dissolve 1 g of the sample in 99 g of dimethyl sulfoxide to prepare a sample solution with a mass fraction of 1%, and carry out the following test.

2. Evaluation test on the effect of inhibiting tyrosinase activity

Accurately draw the sample solution, phosphate buffered saline solution (PBS) with a pH value of 6.8 and dopa solution with a mass fraction of 0.1% according to Table 4. The tyrosinase solution was incubated at 30°C for 10 min, quickly transferred to a cuvette, and the absorbance was measured at 475 nm.

Table 4

Reaction solution composition Group 1 2 teams 3 groups 4 groups Sample solution /mL the the 1 1 Phosphate buffer solution /mL 2 2.5 1 1.5 Dopa solution /mL 1 1 1 1 Tyrosinase solution /mL 0.5 the 0.5 the total capacity /mL 3.5 3.5 3.5 3.5

Calculate the relative inhibition rate of the sample to tyrosinase according to formula (1) ( I , referred to as the inhibition rate, the same below).

I =[(A1-A2)-(A3-A4)]×100%÷(A1-A2) (1)

In the formula: A1, A2, A3, and A4 are the absorbance of the solutions from Group 1 to Group 4, respectively.

3. Inhibition of melanin synthesis in melanocytes

B16 melanoma cells were seeded in a 96-well plate at a density of 1×10 ⁵ cells/mL, 90 μL per well, and after incubation in a CO2 incubator for 24 hours, the sample solution was added to each well. At the same time, a blank control group of culture medium and cells was set up.

After the culture plate was incubated in an incubator for 72 hours, the supernatant was discarded, washed twice with PBS, and then 0.5 mL of trypsin was added to each well to digest the cells for 3 min, and 2 mL of maintenance solution was added to each well to stop the digestion. After mixing, take out 0.5mL of each concentration for cell counting. The rest of the cell suspension was centrifuged at 2500r/min for 5min, the supernatant was discarded, NaOH solution was added to the precipitate, heated to dissolve the melanin, and the absorbance value was measured with an enzyme-linked immunosorbent assay at a wavelength of 490nm. According to formula (2) to calculate the inhibition rate of melanin synthesis ( I ').

I '=(1-A5÷P1/A6÷P2)×100% (2)

In the formula: A5 is the absorbance value of the drug well; P1 is the cell density of the drug well; A6 is the absorbance value of the control well; P2 is the cell density of the control well.

4. Evaluation test of superoxide anion free radical scavenging effect

Add liquid medicine according to Table 5. Take 2 mL of the sample solution, add 2.25 mL of Tris-HCl buffer solution (pH 8.36), 0.25 mL of pyrogallol solution with a concentration of 10 mmol/L, put it in a water bath at 25 °C, and add 10 mol/L hydrochloric acid dropwise at the 4th minute. drops to terminate the reaction. Absorbance was measured at 420 nm with a UV spectrophotometer. Calculate the clearance rate (D) according to formula (3).

D=[1-(A9-A10)÷(A7-A8)]×100% （3）

In the formula: A7 is the absorbance value of O2- produced by the spontaneous oxidation reaction of pyrogallol under Tris-HCl solution; A8 is the absorbance value of Tris-HCl solution; A9 is the absorbance value of the scavenging O2- reaction; A10 is the Absorbance values.

table 5

response group Tris-HCl Pyrogallol sample solution Deionized water F ₀ 2.25mL 0.25mL 0 mL 2mL F ₁ 2.25mL 0.25mL 2mL 0 mL F ₂ 2.25mL 0 mL 2mL 0 mL

5. Anti-ultraviolet effect evaluation test

The test was carried out in accordance with the light industry standard QB/T 2334-1997 of the People's Republic of China (Qualitative Determination of UV Absorbers in Cosmetics-UV Spectrophotometry). Dilute the sample solution 10 times with ethanol, then scan every 10nm in the range of 280~400nm, determine the absorbance at each wavelength, and calculate the average absorbance.

6. Experimental results

Whitening skin care effect evaluation experiment is shown in Table 6.

Table 6

Experimental results show that the Chinese medicine extract composition of the present invention can significantly inhibit tyrosinase activity and melanin synthesis, scavenge free radicals and anti-ultraviolet function, and have obvious improvement than the effect of single Chinese medicine extract, It shows that different traditional Chinese medicines have played a synergistic effect.

Experimental example 2 Human trial evaluation experiment of whitening skin care milk

1. Experimental method

The whitening skin care milk prepared in Example 3 and the ordinary skin care milk prepared in Comparative Example 6 were tested and evaluated on human body respectively. A total of 40 healthy volunteers (20 males and 20 females) aged 20-50 were selected. The method of use is to use after cleansing the face every day, twice a day (once in the morning and before going to bed at night), use the whitening skin care milk prepared according to Example 3 on the left face, and use the ordinary skin care milk prepared in Comparative Example 6 on the right face . The experiment period is one month, and the skin melanin of the volunteers' faces is regularly measured, and the samples are evaluated for moisture retention, gloss and whitening degree during use, with the highest score being 5 and the lowest score being 1.

2. Experimental results

Accompanying drawing 1 is the comparison diagram of skin melanin content using whitening skin care milk and common skin care milk. The results of accompanying drawing 1 show that the skin melanin content after using the whitening skin care milk prepared in Example 3 is obviously lower than that after using the ordinary skin care milk prepared in Comparative Example 6.

Fill out the trial evaluation form according to the sensory evaluation. The half-face experiment evaluation form was collected, and the statistical results are shown in Table 7.

Table 7 The average value of human trial evaluation results

Evaluation results Comparative example 6 Example 3 Humidity 3 points 4 points Gloss 2 minutes 5 points whitening degree 1 point 5 points

It can be seen from the results in Table 7 that the skin gloss and whitening degree after using the whitening skin care milk prepared in Example 3 are significantly better than those of the ordinary skin care milk prepared in Comparative Example 6

The above results show that the whitening skin care lotion containing the extract of the traditional Chinese medicine composition of the present invention has a good whitening and nourishing effect, and can quickly restore rough and dull skin to a healthy state, making it smooth and delicate, fair and attractive.

Claims (2)

1. A whitening Chinese medicine composition, characterized in that it comprises the following components in percentage by weight: 20-50% Angelica sinensis, 10-30% white root, 6-18% desmodium, 5-20% sage, 5% licorice ~15%; the preparation method of the extract of the Chinese medicine composition is as follows: take the Chinese medicine raw materials Angelica sinensis, Baiji, Desmodium, Clary Sage, and Glycyrrhizae according to the proportioning, pulverize the above-mentioned Chinese medicine composition, sieve, and press the liquid-material mass ratio Add extraction solvent at a ratio of 10:1 to 30:1. After soaking for 1 to 2 hours, use a high-pressure homogeneous extractor to extract at room temperature under a pressure of 50 to 100 MPa. After the extract is centrifuged, it is absorbed by a macroporous resin. 95% ethanol was eluted multiple times, the eluate was collected, concentrated under reduced pressure until the mass ratio of the extract to the traditional Chinese medicine was 1:0.5 to 1:10, and then dissolved in butanediol with the same quality as the extract concentrate, filtered, The extract of the traditional Chinese medicine composition is obtained; in order to avoid solvent residue, the extraction solvent used in the present invention can adopt water, ethanol, ethanol aqueous solution. 2. the application of the extract of the Chinese medicine composition described in claim 1 in the preparation of cosmetics, it is characterized in that the extract of the above-mentioned Chinese medicine composition is contained in the cosmetics, and its consumption is 0.01 ~ 20%, and the preferred consumption is 1 ~ 10% .