CN103960540B - Cattle feed additive and application method thereof - Google Patents
Cattle feed additive and application method thereof Download PDFInfo
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- CN103960540B CN103960540B CN201410208462.2A CN201410208462A CN103960540B CN 103960540 B CN103960540 B CN 103960540B CN 201410208462 A CN201410208462 A CN 201410208462A CN 103960540 B CN103960540 B CN 103960540B
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- 241000283690 Bos taurus Species 0.000 title claims abstract description 52
- 238000000034 method Methods 0.000 title claims abstract description 27
- 239000003674 animal food additive Substances 0.000 title claims abstract description 19
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims abstract description 33
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims abstract description 33
- 239000001630 malic acid Substances 0.000 claims abstract description 33
- 235000011090 malic acid Nutrition 0.000 claims abstract description 33
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- 235000020661 alpha-linolenic acid Nutrition 0.000 description 2
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- Fodder In General (AREA)
Abstract
The invention provides a cattle feed additive and an application method thereof, particularly a nutrition regulation method for increasing the concentration of propionic acid in Yanbian cattle rumen and the content of conjugated linoleic acid (CLA) and an endogenous synthesized precursor thereof---trans-oleic acid (trans-11C18:1) by adding linseed oil and malic acid into daily ration. Through a daily ration nutrition technique, the PH value and the propionic acid concentration of the Yanbian cattle rumen are increased to a certain extent, the internal environment of the rumen is improved, and the propionic acid and the trans-oleic acid in rumen liquid are increased.
Description
Technical field
The present invention relates to a kind of ox feed additive and its using method, particularly, add siritch and Herba Marsileae Quadrifoliae in daily ration
Fruit acid is improving Yan-Bian yellow cattle cud propionate concentration and conjugated linoleic acid (CLA) and its endogenous synthesis precursor-antiform oleic acid
(trans-11C18:1) nutrition regulation method of content.By diet nutrient technology, Yan-Bian yellow cattle cud PH, propionate concentration have
Increased, improved Rumen Internal Environment, increased conjugated linoleic acid and antiform oleic acid in rumen fluid.Belong to diet nutrient element
Metabolic regulation application.
Background technology
With pursuit of the people to health food, functional grease also increasingly becomes the focus of domestic and international research.Function
Property oils and fatss be the oils and fatss with special physiological function, the noticeable polyunsaturated fatty acid of comparison mainly has linoleic acid, γ-Asia
Numb acid, eicosapentaenoic acid (EPA) and docosahexenoic acid (DHA), and some phospholipid such as lecithin, cephalin etc..Wherein,
Linoleic acid is essential fatty acid；Gamma-Linolenic acid has the specific function for reducing cholesterol；EPA and DHA can reduce platelet aggregation,
Reduction blood fat, prevents coronary heart disease；DHA also has the effects such as improving memory, judgment, prevent brain aging, and has suppression cancer effect
Really；Cephalin and lecithin are lipids, have important regulation to biomembranous biological activity and the homergy in body
Function.With research of the people to lipidic biomass activity, natural novel fatty acid-conjugated linoleic acid possesses special life because of it
Thing activity, such as antitumaous effect, atherosclerosis, raising immune function, suppression lipidosiss, so as to indirect supply people
Class needs, and the health level of the mankind is improved, with substantial worth.Ruminant source food is that the mankind are taken in by wholefood
The unique channels of CLA.Therefore improving the conjugated fatty acid content in ruminant body breast, meat becomes the heat of current nutrition educational circles research
Point.
The source of CLA mainly has two kinds in ruminant animal fat：One kind is from unsaturated fatty acids such as linoleic acids in tumor
Intermediate product in stomach hydrogenation process；Another kind is the unsaturated fatty acids such as linoleic acid plus linolenic acid in microorganism hydrogenation process
Intermediate product trans-11C18:1, further desaturation shape in the presence of the desaturases of △ 9 in being organized by ruminant body
Into.It was found that CLA can be by the internal dehydrogenase of Δ 9, by trans-11C18:1 is transformed, and by milch cow abomasum fistula
Pipe irrigates trans-11C18:1, the CLA for as a result making milch cow improves 31%.It can be seen that CLA is mainly via trans-11C18:1 closes
Into.CLA contents depend on daily ration Linoleic acid and linolenic acid content height, CLA and trans-11C18 in cud:1 yield
And tissue in the desaturases of △ 9 vigor.
Malic acid is the intermediate product of ruminal metabolism succinic acid-propanoic acid approach, and in vitro tests research shows that malic acid has
The effect of similar ion carrier antibiotic, can improve rumen zymosiss type.Malic acid can stimulate the utilization of lactic acid, increase tumor
The yield of the yield and propanoic acid of stomach pH value and general volatile fatty acid.The present invention adds siritch and malic acid with varying level
In being added on daily ration, what research siritch and malic acid have affect on Yan-Bian yellow cattle rumen zymosiss, is development functionality high quality beef
Theoretical foundation is provided.
The content of the invention
It is an object of the invention to provide a kind of method of the adequate diet of addition siritch and malic acid.By this method
The propionate concentration in Yan-Bian yellow cattle cud can be improved, CLA and trans-11C18 is made:1 content rises, and to a certain degree
On improve Rumen Internal Environment, provide theoretical foundation for development functionality high quality beef.
The present invention provide it is a kind of for improving Yan-Bian yellow cattle cud in conjugated linoleic acid and antiform oleic acid concentration method, its
It is characterised by, feed additive is added in the feedstuff of Yan-Bian yellow cattle, and the feed additive contains the linum of 2～5 weight portions
Oil, the malic acid of 1.6～4.8 weight portions, and Yan-Bian yellow cattle is fed in the way of in the of 9 hours by interval daily.
The present invention also provide it is a kind of for improving Yan-Bian yellow cattle cud in propionate concentration method, it is characterised in that prolonging
Add feed additive in the feedstuff of side cattle, the feed additive contains the siritch of 2～5 weight portions and 1.6～4.8 weights
The malic acid of amount part, and fed Yan-Bian yellow cattle in the way of interval by daily 9 hours.
The present invention further provides in a kind of meat for improving Yan-Bian yellow cattle conjugated fatty acid content method, its feature exists
In, feed additive is added in the feedstuff of Yan-Bian yellow cattle, the consumption of the feed additive is：In terms of dry matter basis, Herba Marsileae Quadrifoliae
80～240g/d of fruit acid, siritch 475g/d.
The method of conjugated fatty acid content in the meat for improving Yan-Bian yellow cattle of the present invention, it is characterised in that the feeding
The consumption of feed additives is：In terms of dry matter basis, malic acid 160g/d, siritch 475g/d.Cattle feedstuff of the present invention,
In the daily ration, under dry matter basis, concentrate is 60 with the ratio of coarse fodder:40 or other ratios.
Cattle feedstuff of the present invention, it is characterised in that suitable for Yan-Bian yellow cattle.
The present invention provides a kind of ox feed additive, it is characterised in that relative to the daily ration of 100 weight portions, containing 2～5
The siritch of weight portion.
Ox feed additive of the present invention, it is characterised in that relative to the daily ration of 100 weight portions, also containing 1.6～
The malic acid of 4.8 weight portions.
The present invention also provides a kind of using method of ox feed additive, it is characterised in that relative to the day of 100 weight portions
Grain, adds the siritch of 2～5 weight portions.
The using method of ox feed additive of the present invention, it is characterised in that relative to the daily ration of 100 weight portions,
Also add the malic acid of 1.6～4.8 weight portions.
The present invention provides a kind of cattle feedstuff again, it is characterised in that relative to the daily ration of 100 weight portions, containing 2～5 weight
The siritch of part.
Cattle feedstuff of the present invention, it is characterised in that relative to the daily ration of 100 weight portions, also containing 1.6～4.8 weights
The malic acid of amount part.
Cattle feedstuff of the present invention, it is characterised in that the daily ration includes concentrate supplement, corn silage and Radix Glycyrrhizae.
Cattle feedstuff of the present invention, it is characterised in that in the daily ration, under dry matter basis, concentrate and coarse fodder it
Than for 60:40 or other ratios.
It is of the present invention, it is characterised in that suitable for Yan-Bian yellow cattle.
Beneficial effect of the present invention：By to ruminant, particularly being adjusted using nutrition of the present invention to Yan-Bian yellow cattle
Prosecutor method and forage compounding, can effectively improve the propionate concentration in Yan-Bian yellow cattle cud, make CLA and its endogenous synthesis precursor
Thing trans-11C18:1 improves, and improves Rumen Internal Environment to a certain extent, is conducive to improving Yan-Bian yellow cattle production performance.
Specific embodiment
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is described in detail, it is clear that
Described embodiment is only a part of embodiment of the invention, rather than the embodiment of whole.Based on the enforcement in the present invention
Example, the every other embodiment that those of ordinary skill in the art are obtained on the premise of creative work is not made all belongs to
In the scope of protection of the invention.
It should be noted that herein, term " including ", "comprising" or its any other variant are intended to non-row
His property is included, so that a series of process, method, article or equipment including key elements not only include those key elements, and
And also include other key elements being not expressly set out, or also include for this process, method, article or equipment institute inherently
Key element.
In order to realize object above, the technical solution used in the present invention is to add linum in the original feedstuff of Yan-Bian yellow cattle
Oil and malic acid.
The present invention is feeds utilized to be made up of basal diet with siritch and malic acid, and daily ration essence is thick than being 60:40.Basic day
Grain scale of feeding is made up of concentrate supplement, corn silage, Radix Glycyrrhizae, its formula
And trophic level is shown in Tables 1 and 2：The daily ration concentrate of table 1 is constituted and trophic level
Note：Above items are dry matter basis (%)
The daily ration coarse fodder of table 2 is constituted and trophic level
Note：Above items are dry matter basis (%)
Embodiment 1
1. the production screening of cattle：
Kind：Yan-Bian yellow cattle
2. feeding environment：
Feed (7 at twice daily:30 and 16:30), free water, conventional illumination.Concentrate is mixed with coarse fodder before feeding
Uniformly.Every time after feeding, cowshed is cleaned, keep cowshed dry cleansing, it is ensured that cattle has cleaning, clean drinking-water environment.
3. feeding process：
Under dry matter basis, daily ration essence is thick than being 60:40.Basal diet scale of feeding is by concentrate supplement, corn silage, dry
Grass composition.Add malic acid 80g/d, 160g/d and 240g/d (dry matter basis) respectively in daily ration.
Embodiment 2
1. the production screening of cattle：
Kind：Yan-Bian yellow cattle
2. feeding environment：
Feed (7 at twice daily:30 and 16:30), free water, conventional illumination.Concentrate is mixed with coarse fodder before feeding
Uniformly.Every time after feeding, cowshed is cleaned, keep cowshed dry cleansing, it is ensured that cattle has cleaning, clean drinking-water environment.
3. feeding process：
Under dry matter basis, daily ration essence is thick than being 60:40.Basal diet scale of feeding is by concentrate supplement, corn silage, dry
Grass composition, its formula and trophic level are shown in Tables 1 and 2.Addition siritch 475g/d (dry matter basis).
Embodiment 3
1. the production screening of cattle：
Kind：Yan-Bian yellow cattle
2. feeding environment：
Feed (7 at twice daily:30 and 16:30), free water, conventional illumination.Concentrate is mixed with coarse fodder before feeding
Uniformly.Every time after feeding, cowshed is cleaned, keep cowshed dry cleansing, it is ensured that cattle has cleaning, clean drinking-water environment.
3. feeding process：
Under dry matter basis, daily ration essence is thick than being 60:40.Basal diet scale of feeding is by concentrate supplement, corn silage, dry
Grass composition, its formula and trophic level are shown in Tables 1 and 2.Addition malic acid 160g/d+ siritch 475g/d (dry matter basis).
Comparative example 1
1. the production screening of cattle：
Kind：Yan-Bian yellow cattle
2. feeding environment：
Feed (7 at twice daily:30 and 16:30), free water, conventional illumination.Concentrate is mixed with coarse fodder before feeding
Uniformly.Every time after feeding, cowshed is cleaned, keep cowshed dry cleansing, it is ensured that cattle has cleaning, clean drinking-water environment.
3. feeding process：
Under dry matter basis, daily ration essence is thick than being 60:40.Basal diet scale of feeding is by concentrate supplement, corn silage, dry
Grass composition, its formula and trophic level are shown in Tables 1 and 2.
Test example 1：Impact of the daily ration addition Fructus Tsaoko acid to Yan-Bian yellow cattle cud VFA changes
Table 3 adds malic acid and impact of the siritch to rumen fluid ph value changes
Test example 2：Daily ration adds malic acid and impact of the siritch to rumen fluid ph value changes
The change of rumen ph is from table 4, and the rumen ph variation tendency after each group feeding is similar.Embodiment 1,2 with compare
Example 1 is compared pH value and is improved trend, and 9h improves notable after feeding.Each group PH meansigma methodss are respectively：6.70 (embodiments 2),
6.78 (embodiments 3), wherein 6.52 (comparative examples 1), comparative example 1 are minimum.Excursion is between 6.40～6.88.
Table 4 adds malic acid and impact of the siritch to rumen fluid ph value changes
Note：Colleague's data shoulder mark lower case is different to represent significant difference (P ＜ 0.05)
Test example 3：Daily ration adds malic acid and impact of the siritch to NH3-N concentration in rumen fluid
The change of cud NH3-N is from table 5, and the cud NH3-N variation tendencies after each group feeding are similar, but comparative example
1 and the amplitude of embodiment 2 it is larger.NH3-N concentration is gradually reduced i.e. after feeding, reaches minimum in 3h～6h, then gradually
Raise and be close to the level before feeding.Each embodiment 2 and 3 compares with comparative example 1, and embodiment 3 is significantly reduced in 1h.Excursion
Between 7.61～20.87mg/100ml.
Table 5 adds malic acid and impact (unit of the siritch to NH3-N concentration in rumen fluid：mg/100ml)
Note：Colleague's data shoulder mark lower case is different to represent significant difference (P ＜ 0.05)
Test example 4：Daily ration adds impact of the malic acid with siritch to VFA changes in rumen fluid
The change of cud VFA concentration from table 5, general volatile fatty acid (TVFA) concentration change scope 60.40～
Between 78.77mmol/L, each group variation tendency is all consistent, and 3h reaches maximum after feeding, is then gradually lowered and is close to
Level before feeding.
The change of acetic acid concentration, each group variation tendency is similar, except embodiment 1 is that 6h reaches peak after feeding, other
Group 3h after feeding reaches maximum, the level for being then all gradually lowered and being close to before feeding.Embodiment 2, embodiment 3 with than
Compare compared with example 1, embodiment 2 is in 1h, 3h, 6h significant difference, the change of propionate concentration, the variation tendency of each group concentration and total volatilization
Property fatty acid variation tendency it is consistent, i.e., all reach maximum in 3h, the level for being then gradually lowered and being close to before feeding.Each
Time point, the concentration highest of embodiment 2, comparative example 1 is minimum.During 3h, each test group compares difference significantly (P ＜ in comparative example 1
0.05)。
The change of butyric acid density, the variation tendency of each group concentration is consistent with the variation tendency of general volatile fatty acid, each
The concentration highest of time point matched group.Embodiment 2 is substantially less than comparative example 1 and embodiment 2 (P ＜ 0.05) during 9h.
The change of acetic acid/propanoic acid, each group variation tendency is similar, equally reaches maximum in 6h, is gradually reduced afterwards.Implement
Example 2, significant difference (the P ＜ 0.05) compared with comparative example 1 of embodiment 3.
The change (mmol/L) of volatile fatty acid (VFA) concentration in the rumen fluid of table 6
The change of rumen ph is begun to decline from table 4, each group pH after morning feeding, and 3h reaches minimum, then gradually returns
Rise, excursion is between 6.40～6.88.The preferable acidity of cud is 6.4-6.8, in neutrality to faintly acid.In addition linum
On the basis of oil, then adding malic acid becomes apparent from the rising of rumen fluid pH value.In the present invention, each time point, the pH of each group exist
Within normal range, the pH scopes for not affecting cud normal fermentation are illustrated.
NH3-N concentration change scope is 7.61～20.87mg/100ml in the present invention.NH3-N concentration each group in the present invention
Between except the embodiment 1 in 1h, each time point difference it is not notable.The ammonia nitrogen concentration of embodiment 1 each time point after feeding
Fall is maximum.Thalline egg is synthesized by because NH3-N concentration depends on protein degradation speed in feedstuff with microorganism
White ability, illustrates that embodiment 2 is used for the enforcement that the ability of synthetic animalcule albumen compares common addition fatty acid and malic acid
It is relatively strong for example 3.Adding malic acid in daily ration can reduce cud NH3-N concentration.The present invention at the same time add fatty acid and
Bigger with the amplitude that other groups compare decline during malic acid, it is consistent that this is probably the two impact for producing.
In common addition fatty acid and malic acid, in rumen fluid in addition to propanoic acid increased, other VFA drop for this experiment
Low, second/the third ratio is significantly reduced (P ＜ 0.05).
Test example 5：Daily ration adds the impact that siritch is constituted with malic acid to rumen fluid fatty acid
From table 7, t11C18:The content of 1, c9, t11-CLA and t10, c12-CLA highest in 1h.The He of embodiment 2
The c9-C18 of embodiment 3:1st, c9, t11-CLA, t10, c12-CLA groups content of fatty acid is all higher than comparative example 1.
The change (mg/50ml) of content of fatty acid in the rumen fluid of table 7
Table 9 Contents(mg/50ml)of C18-fatty acids in rumen fluid
Note：Colleague's data shoulder mark lower case is different to represent significant difference (P ＜ 0.05)
Add siritch and malic acid in daily ration to Yan-Bian yellow cattle cud PH, propionate concentration has the trend for increasing, but to second
Acid, butanoic acid and TVFA have the trend of reduction, while being even more for addition is obvious.And cud NH3-N concentration is not made significant difference.To sum up
It is described, add the rumen zymosiss that siritch and malic acid are conducive to Yan-Bian yellow cattle in diet, and while additive effect more preferably.
Claims (2)
1. it is a kind of for improving Yan-Bian yellow cattle cud in conjugated linoleic acid and antiform oleic acid concentration method, it is characterised in that
Add feed additive in the feedstuff of Yan-Bian yellow cattle, the feed additive contain the siritch of 2～5 weight portions, 1.6～4.8
The malic acid of weight portion, and Yan-Bian yellow cattle is fed in the way of in the of 9 hours by interval daily.
2. it is a kind of for improving Yan-Bian yellow cattle cud in propionate concentration method, it is characterised in that in the feedstuff of Yan-Bian yellow cattle
Addition feed additive, the feed additive contains the malic acid of the siritch of 2～5 weight portions and 1.6～4.8 weight portions,
And fed Yan-Bian yellow cattle in the way of interval by daily 9 hours.
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| CN105410389A (en) * | 2015-12-29 | 2016-03-23 | 扬州大学 | Aliphatic acid composition rumen regulating agent raising ruminant fattening performance |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1762227A (en) * | 2005-11-09 | 2006-04-26 | 中国农业科学院畜牧研究所 | Nutrition regulation method and feed for increasing milk conjugated linoleic acid (CLA) content |
| CN101731485A (en) * | 2010-02-23 | 2010-06-16 | 浙江科盛饲料股份有限公司 | Formula and production process of dairy cow protein particle feed |
| CN102283321A (en) * | 2010-06-18 | 2011-12-21 | 哈尔滨爱特杰牧业有限公司 | Cow rumen fermentation accelerator |
| CN102488119A (en) * | 2011-12-28 | 2012-06-13 | 河南宏展实业有限公司 | Cow feedstuff additive, cow feedstuff, and preparation methods thereof |
| CN103125752A (en) * | 2011-11-21 | 2013-06-05 | 南通中科睿智科技服务有限公司 | Biochemical increasing milk preparation of dairy cow |
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| KR100431906B1 (en) * | 2001-10-16 | 2004-05-17 | 주식회사 애그리브랜드 퓨리나코리아 | Feed increasing CLA content in the milk and manufacturing method thereof |
| AU2002351537A1 (en) * | 2002-10-31 | 2004-06-18 | Piotr Zajac | Feed additive and process for manufacturing thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1762227A (en) * | 2005-11-09 | 2006-04-26 | 中国农业科学院畜牧研究所 | Nutrition regulation method and feed for increasing milk conjugated linoleic acid (CLA) content |
| CN101731485A (en) * | 2010-02-23 | 2010-06-16 | 浙江科盛饲料股份有限公司 | Formula and production process of dairy cow protein particle feed |
| CN102283321A (en) * | 2010-06-18 | 2011-12-21 | 哈尔滨爱特杰牧业有限公司 | Cow rumen fermentation accelerator |
| CN103125752A (en) * | 2011-11-21 | 2013-06-05 | 南通中科睿智科技服务有限公司 | Biochemical increasing milk preparation of dairy cow |
| CN102488119A (en) * | 2011-12-28 | 2012-06-13 | 河南宏展实业有限公司 | Cow feedstuff additive, cow feedstuff, and preparation methods thereof |
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