CN103849458B - Extraction method of microalgae oil - Google Patents
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- 238000000605 extraction Methods 0.000 title claims abstract description 40
- 239000003960 organic solvent Substances 0.000 claims abstract description 39
- 239000007787 solid Substances 0.000 claims description 30
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 24
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 22
- 238000009835 boiling Methods 0.000 claims description 15
- 238000005119 centrifugation Methods 0.000 claims description 15
- 239000012074 organic phase Substances 0.000 claims description 15
- 239000000243 solution Substances 0.000 claims description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 10
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 10
- 239000011259 mixed solution Substances 0.000 claims description 4
- 241000214658 Tetraodon fluviatilis Species 0.000 claims 1
- 239000002904 solvent Substances 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 18
- 150000007524 organic acids Chemical class 0.000 abstract description 11
- 239000007788 liquid Substances 0.000 abstract description 4
- 235000014593 oils and fats Nutrition 0.000 abstract description 4
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- 239000002253 acid Substances 0.000 abstract description 2
- 239000000284 extract Substances 0.000 abstract description 2
- 230000003834 intracellular effect Effects 0.000 abstract description 2
- 239000002351 wastewater Substances 0.000 abstract description 2
- 238000003912 environmental pollution Methods 0.000 abstract 1
- 229910052500 inorganic mineral Inorganic materials 0.000 abstract 1
- 239000011707 mineral Substances 0.000 abstract 1
- 239000003921 oil Substances 0.000 description 56
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 21
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 14
- 239000000203 mixture Substances 0.000 description 12
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 10
- 238000003756 stirring Methods 0.000 description 10
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 8
- 150000007522 mineralic acids Chemical class 0.000 description 8
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 8
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 7
- 235000019253 formic acid Nutrition 0.000 description 7
- 240000009108 Chlorella vulgaris Species 0.000 description 6
- 238000002156 mixing Methods 0.000 description 5
- 241001536324 Botryococcus Species 0.000 description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
- 241001491687 Thalassiosira pseudonana Species 0.000 description 4
- 241000957276 Thalassiosira weissflogii Species 0.000 description 4
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 4
- 239000003208 petroleum Substances 0.000 description 4
- 235000007089 Chlorella vulgaris Nutrition 0.000 description 3
- 239000002551 biofuel Substances 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 241000043665 Clenchiella minutissima Species 0.000 description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 2
- 239000003225 biodiesel Substances 0.000 description 2
- 239000000446 fuel Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 235000005985 organic acids Nutrition 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- 238000010306 acid treatment Methods 0.000 description 1
- -1 aliphatic organic acid Chemical class 0.000 description 1
- 238000000498 ball milling Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- AZHSSKPUVBVXLK-UHFFFAOYSA-N ethane-1,1-diol Chemical compound CC(O)O AZHSSKPUVBVXLK-UHFFFAOYSA-N 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
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- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 235000019260 propionic acid Nutrition 0.000 description 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Production Of Liquid Hydrocarbon Mixture For Refining Petroleum (AREA)
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Abstract
Description
技术领域technical field
本发明涉及可再生资源领域和生物化工领域，具体地说，涉及一种微藻油脂的提取方法。The invention relates to the fields of renewable resources and biochemical industry, in particular to a method for extracting microalgae oil.
背景技术Background technique
近年来，由于能源短缺和环境破坏的日益严重，发展生物能源已成为全球关注的焦点。其中，以可再生油脂为原料的生物燃油（生物柴油或航空燃油等）发展备受关注。想要发展以可再生油脂为原料的生物燃油，油脂的可持续供应是关键。近年来，微藻油脂作为生物燃油原料受到国内外专家和学者广泛关注。微藻具有生命周期短，生长速率快，可以适应各种各样的种植条件，并且可以全年采收。另外，微藻培育占用面积少，可以不利用一般农用耕地，节约水资源，是一种非常有前景的可再生油脂原料。然而，绝大多数微藻油脂都在胞内，如何有效破碎微藻细胞并进一步将胞内的油脂提取出来是促进微藻油脂在生物能源领域应用的关键。In recent years, due to the increasingly severe energy shortage and environmental damage, the development of bio-energy has become the focus of global attention. Among them, the development of biofuels (biodiesel or aviation fuel, etc.) based on renewable oils and fats has attracted much attention. A sustainable supply of oil is key to the development of biofuels derived from renewable oils. In recent years, microalgae oil has been widely concerned by experts and scholars at home and abroad as a raw material for biofuel. Microalgae have a short life cycle, fast growth rate, can adapt to a variety of planting conditions, and can be harvested throughout the year. In addition, the cultivation of microalgae takes up less area, so it does not need to use general agricultural land and saves water resources. It is a very promising renewable oil raw material. However, the vast majority of microalgal oils are in the cells. How to effectively break the microalgal cells and further extract the intracellular oils is the key to promote the application of microalgal oils in the field of bioenergy.
目前微藻油脂的提取主要为机械法破碎细胞，然后再用有机溶剂抽提，以及先用化学法破碎细胞再用有机溶剂抽提。其中，机械破碎法包括高压匀浆法、超声破碎法、球磨法、微波法等，这些破碎细胞的方法存在能耗大，设备投资大，难以在工业化生产中进一步应用；而化学法主要采用无机酸处理法，一般用强无机酸如硫酸、盐酸等，无机酸破碎细胞存在无机酸用量大（一般用量为基于微藻干物质质量的10%以上），无机酸不仅难以回收，而且，高浓度的无机酸对设备腐蚀严重，含酸废液排放对环境也会产生严重影响。可见，迫切需要开发新型的微藻油脂提取工艺来促进微藻油脂在生物柴油及生物航空燃油制备领域中的应用。At present, the extraction of oil from microalgae mainly involves breaking cells mechanically, and then extracting them with organic solvents, or first breaking cells chemically, and then extracting them with organic solvents. Among them, the mechanical crushing method includes high-pressure homogenization method, ultrasonic crushing method, ball milling method, microwave method, etc. These methods of crushing cells have high energy consumption and large investment in equipment, so it is difficult to further apply them in industrial production; while chemical methods mainly use inorganic Acid treatment method generally uses strong inorganic acids such as sulfuric acid, hydrochloric acid, etc. The amount of inorganic acids used to break cells with inorganic acids is large (the general amount is more than 10% based on the dry matter of microalgae), and inorganic acids are not only difficult to recover, but also high concentration The inorganic acid in the equipment is severely corroded, and the discharge of acid-containing waste liquid will also have a serious impact on the environment. It can be seen that there is an urgent need to develop a new microalgae oil extraction process to promote the application of microalgae oil in the field of biodiesel and bioaviation fuel preparation.
发明内容Contents of the invention
本发明的目的是提供一种产油微藻的油脂提取方法。The purpose of the present invention is to provide a method for extracting oil from oleaginous microalgae.
为了实现本发明目的，本发明的一种微藻油脂的提取方法，按kg：L为1:3-20的固液比向微藻中加入质量百分比浓度30%-100%的有机酸以及质量百分比浓度0%-3%的无机酸，处理0.25-5.0h后，加入有机溶剂进行油脂的提取。In order to achieve the purpose of the present invention, a method for extracting microalgae oil of the present invention, according to the solid-to-liquid ratio of 1:3-20 in kg:L, add organic acids with a mass percentage concentration of 30%-100% and mass percent organic acids to the microalgae. Inorganic acid with percentage concentration of 0%-3%, after treatment for 0.25-5.0h, add organic solvent to extract oil.
前述提取方法中，所述有机溶剂可与有机酸同时加入微藻中。In the aforementioned extraction method, the organic solvent and the organic acid can be added to the microalgae simultaneously.
前述提取方法中，按kg：L为1:2-10的固液比向微藻中加入有机溶剂。In the aforementioned extraction method, an organic solvent is added to the microalgae at a solid-to-liquid ratio of 1:2-10 in kg:L.
前述提取方法中，所述有机酸为脂肪族有机酸。包括但不限于甲酸、乙酸和/或丙酸等。In the aforementioned extraction method, the organic acid is an aliphatic organic acid. Including but not limited to formic acid, acetic acid and/or propionic acid, etc.
前述提取方法中，所述无机酸包括但不限于硫酸、盐酸、磷酸和/或硝酸等。In the aforementioned extraction method, the inorganic acid includes but not limited to sulfuric acid, hydrochloric acid, phosphoric acid and/or nitric acid and the like.
前述提取方法中，所述有机溶剂包括但不限于氯仿、石油醚和/或正己烷等，以及它们与甲醇或乙醇的混合液。In the aforementioned extraction method, the organic solvent includes but is not limited to chloroform, petroleum ether and/or n-hexane, etc., and their mixed solution with methanol or ethanol.
本发明中涉及的微藻包括但不限于Chlorella vulgaris、Botryococcus sp.、C.vulgaris、C.minutissima、T.fluviatilis、T.pseudonana等。Microalgae involved in the present invention include, but are not limited to, Chlorella vulgaris, Botryococcus sp., C. vulgaris, C. minutissima, T. fluviatilis, T. pseudonana, and the like.
本发明首次提供一种微藻油脂提取的新工艺，即用有机酸破碎细胞，然后再用有机溶剂对胞内的油脂进行提取；所述有机酸和有机溶剂也可同时加入。采用本工艺，可得到收率大于90%以上的油脂，且有机酸易于回收并循环使用，减少废水排放，对设备要求低，与传统微藻油脂提取工艺相比，该工艺操作简单，对环境污染小，具有很好的工业化应用前景。The present invention firstly provides a new process for oil extraction of microalgae, that is, breaking cells with organic acid, and then extracting oil in cells with organic solvent; the organic acid and organic solvent can also be added at the same time. Using this process, oil with a yield of more than 90% can be obtained, and the organic acid is easy to recover and recycle, reducing waste water discharge, and has low requirements for equipment. Compared with the traditional microalgae oil extraction process, this process is simple to operate and environmentally friendly. The pollution is small, and it has a good industrial application prospect.
具体实施方式detailed description
以下实施例用于说明本发明，但不用来限制本发明的范围。若未特别指明，实施例中所用的技术手段为本领域技术人员所熟知的常规手段，所用原料均为市售商品。The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are all commercially available products.
实施例1微藻油脂的提取方法The extraction method of embodiment 1 microalgae oil
用质量百分比浓度为60%的甲酸溶液与微藻Chlorella vulgaris按液固比10：1（L:kg）混合后，在常压沸点下搅拌处理1.5小时，再用有机溶剂氯仿/甲醇（体积比1:1）进行油脂抽提，有机溶剂与微藻的液固比（L:kg）为3：1，离心后有机相中可得到收率为92%的油脂。实施例2微藻油脂的提取方法After mixing the formic acid solution with a mass percentage concentration of 60% and the microalgae Chlorella vulgaris at a liquid-solid ratio of 10:1 (L:kg), stir at the boiling point of normal pressure for 1.5 hours, and then use the organic solvent chloroform/methanol (volume ratio 1:1) for oil extraction, the liquid-solid ratio (L:kg) of the organic solvent to the microalgae is 3:1, and the oil with a yield of 92% can be obtained in the organic phase after centrifugation. The extraction method of embodiment 2 microalgae oil
用质量百分比浓度为70%的甲酸溶液与微藻Botryococcus sp.按液固比为6：1（L:kg）混合，同时加入有机溶剂氯仿/甲醇（体积比1:1），有机溶剂与微藻的液固比为（L:kg）为3：1，在常压沸点下搅拌处理2小时，离心后有机相中可得到收率为94%的油脂。Mix the formic acid solution with a mass percentage concentration of 70% and the microalgae Botryococcus sp. at a liquid-solid ratio of 6:1 (L:kg), and add the organic solvent chloroform/methanol (volume ratio 1:1), organic solvent and microalgae The liquid-solid ratio of the algae is (L:kg) 3:1. It is stirred at the boiling point of normal pressure for 2 hours, and the oil with a yield of 94% can be obtained in the organic phase after centrifugation.
实施例3微藻油脂的提取方法The extraction method of embodiment 3 microalgae oil
用质量百分比浓度为80%的乙酸溶液与微藻Chlorella vulgaris按液固比8：1（L:kg）混合后，在常压沸点下搅拌处理3小时，再用有机溶剂氯仿/乙醇（体积比1:1）进行油脂抽提，有机溶剂与微藻的液固比（L:kg）为10：1，离心后有机相中可得到收率为95%的油脂。Mix the 80% acetic acid solution with the microalgae Chlorella vulgaris at a liquid-solid ratio of 8:1 (L:kg), stir for 3 hours at the boiling point of normal pressure, and then use the organic solvent chloroform/ethanol (volume ratio 1:1) for oil extraction, the liquid-solid ratio (L:kg) of the organic solvent to the microalgae is 10:1, and the oil with a yield of 95% can be obtained in the organic phase after centrifugation.
实施例4微藻油脂的提取方法The extraction method of embodiment 4 microalgae oil
向质量百分比浓度为70%的丙酸溶液中添加质量百分比浓度（基于微藻干重）为0.3%的硫酸，，所得混合液与微藻Botryococcus sp.按液固比10：1（L:kg）混合后，在常压沸点下搅拌处理3.0小时，再用有机溶剂正己烷（体积比1:1）进行油脂抽提，有机溶剂与微藻的液固比（L:kg）为4：1，离心后有机相中可得到收率为90%的油脂。Add sulfuric acid with a mass percentage concentration (based on the dry weight of microalgae) of 0.3% to a propionic acid solution with a mass percentage concentration of 70%, and the resulting mixed solution and microalgae Botryococcus sp. are at a liquid-solid ratio of 10:1 (L:kg ) mixed, stirred at the boiling point of normal pressure for 3.0 hours, and then used organic solvent n-hexane (volume ratio 1:1) for oil extraction. The liquid-solid ratio (L:kg) of organic solvent to microalgae was 4:1 , 90% oil can be obtained in the organic phase after centrifugation.
实施例5微藻油脂的提取方法The extraction method of embodiment 5 microalgae oil
向质量百分比浓度为70%的乙酸溶液中添加质量百分比浓度（基于微藻干重）为0.2%的硫酸，所得混合液与微藻C.vulgaris按液固比为4：1（L:kg）混合，同时加入正己烷，有机溶剂与微藻的液固比（L:kg）为6：1，在常压沸点下搅拌处理3.0小时，离心后有机相中可得到收率为90%的油脂。Add sulfuric acid with a mass percentage concentration (based on the dry weight of microalgae) of 0.2% to an acetic acid solution with a mass percentage concentration of 70%, and the liquid-solid ratio of the resulting mixture to the microalgae C.vulgaris is 4:1 (L:kg) Mix and add n-hexane at the same time, the liquid-solid ratio (L:kg) of the organic solvent to the microalgae is 6:1, stir at the boiling point of normal pressure for 3.0 hours, and the oil with a yield of 90% can be obtained in the organic phase after centrifugation .
实施例6微藻油脂的提取方法The extraction method of embodiment 6 microalgae oil
向质量分数30%的甲酸溶液中添加质量百分比浓度（基于微藻干重）为0.5%的盐酸，所得混合液与微藻C.vulgaris，按液固比为10：1（L:kg）混合后，在常压沸点下搅拌处理3.0小时，再用有机溶剂氯仿/乙醇（体积比1:1）进行油脂抽提，有机溶剂与微藻的液固比（L:kg）为8：1，离心后有机相中可得到收率为95%的油脂。Add 0.5% hydrochloric acid (based on the dry weight of the microalgae) to the formic acid solution with a mass fraction of 30%, and mix the resulting mixture with the microalgae C.vulgaris at a liquid-solid ratio of 10:1 (L:kg) Afterwards, stir at the boiling point of normal pressure for 3.0 hours, and then use the organic solvent chloroform/ethanol (volume ratio 1:1) for oil extraction. The liquid-solid ratio (L:kg) of the organic solvent to the microalgae is 8:1. After centrifugation, a 95% oil yield can be obtained in the organic phase.
实施例7微藻油脂的提取方法The extraction method of embodiment 7 microalgae oil
将质量百分比浓度为70%的甲酸溶液与微藻C.minutissima按液固比10：1（L:kg）混合后，在常压沸点下搅拌处理0.25小时，再用有机溶剂石油醚/甲醇（体积比1:1）进行油脂抽提，有机溶剂与微藻的液固比（L:kg）为3：1，离心后有机相中可得到收率为92%的油脂。实施例8微藻油脂的提取方法After mixing the formic acid solution with a mass percentage concentration of 70% and the microalgae C.minutissima at a liquid-solid ratio of 10:1 (L:kg), they were stirred at the boiling point of normal pressure for 0.25 hours, and then the organic solvent petroleum ether/methanol ( Volume ratio 1:1) for oil extraction, the liquid-solid ratio (L:kg) of organic solvent to microalgae is 3:1, and the oil yield can be obtained in the organic phase after centrifugation with a yield of 92%. The extraction method of embodiment 8 microalgae oil
将质量百分比浓度为70%的乙酸溶液与微藻T.fluviatilis按液固比为10：1（L:kg）混合后，在常压沸点下搅拌处理2小时，再用有机溶剂石油醚/甲醇（体积比2:1）进行油脂抽提，有机溶剂与微藻的液固比（L:kg）为10：1，离心后有机相中可得到收率为95%的油脂。Mix the 70% acetic acid solution with the microalgae T.fluviatilis at a liquid-solid ratio of 10:1 (L:kg), stir at the boiling point of normal pressure for 2 hours, and then use the organic solvent petroleum ether/methanol (Volume ratio 2:1) for oil extraction, the liquid-solid ratio (L:kg) of organic solvent to microalgae is 10:1, and the oil yield of 95% can be obtained in the organic phase after centrifugation.
实施例9微藻油脂的提取方法The extraction method of embodiment 9 microalgae oil
在质量百分比浓度为30%的乙酸溶液添加质量百分比浓度（基于微藻干重）为2%的盐酸，所得混合液与微藻T.fluviatilis按液固比为10：1（L:kg）混合后，在常压沸点下搅拌处理2小时，再用有机溶剂氯仿/乙醇醇（体积比3:1）进行油脂抽提，有机溶剂与微藻的液固比（L:kg）为5：1，离心后有机相中可得到收率为98%的油脂。Add hydrochloric acid with a mass percentage concentration (based on the dry weight of microalgae) of 2% to the acetic acid solution with a mass percentage concentration of 30%, and mix the resulting mixture with the microalgae T.fluviatilis at a liquid-solid ratio of 10:1 (L:kg) Finally, stir at the boiling point of normal pressure for 2 hours, and then use the organic solvent chloroform/ethanol alcohol (volume ratio 3:1) for oil extraction. The liquid-solid ratio (L:kg) of the organic solvent to the microalgae is 5:1 , the oil yield of 98% can be obtained in the organic phase after centrifugation.
实施例10微藻油脂的提取方法The extraction method of embodiment 10 microalgae oil
在质量百分比浓度为30%的丙酸溶液添加质量百分比浓度（基于微藻干重）为3%的硫酸，所得混合液与微藻T.pseudonana按液固比为5：1（L:kg）混合后，在常压沸点下搅拌处理3小时，再用有机溶剂氯仿/甲醇（体积比1:1）进行油脂抽提，有机溶剂与微藻的液固比（L:kg）为2：1，离心后有机相中可得到收率为94%的油脂。Add sulfuric acid with a mass percentage concentration (based on the dry weight of microalgae) of 3% to a propionic acid solution with a mass percentage concentration of 30%, and the liquid-solid ratio of the resulting mixture to the microalgae T.pseudonana is 5:1 (L:kg) After mixing, stir at the boiling point of normal pressure for 3 hours, and then use the organic solvent chloroform/methanol (volume ratio 1:1) for oil extraction. The liquid-solid ratio (L:kg) of the organic solvent to the microalgae is 2:1 , the oil yield of 94% can be obtained in the organic phase after centrifugation.
实施例11微藻油脂的提取方法The extraction method of embodiment 11 microalgae oil
将质量百分比浓度为70%的甲酸溶液与微藻T.pseudonana按液固比为8：1（L:kg）混合后，在常压沸点下搅拌处理5小时，再用有机溶剂氯仿/甲醇（体积比2:1）进行油脂抽提，有机溶剂与微藻的液固比（L:kg）为3：1，离心后有机相中可得到收率为96%的油脂。After mixing the formic acid solution with a mass percentage concentration of 70% and the microalgae T.pseudonana at a liquid-solid ratio of 8:1 (L:kg), it was stirred at the boiling point of normal pressure for 5 hours, and then the organic solvent chloroform/methanol ( Volume ratio 2:1) for oil extraction, the liquid-solid ratio (L:kg) of organic solvent to microalgae is 3:1, and the oil yield of 96% can be obtained in the organic phase after centrifugation.
实施例12微藻油脂的提取方法The extraction method of embodiment 12 microalgae oil
在质量百分比浓度为50%的乙酸溶液中添加质量百分比浓度（基于微藻干重）为2%的磷酸，所得混合液与微藻Botryococcus sp.按液固比为5：1（L:kg）混合后，在常压沸点下搅拌处理4.0小时，再用有机溶剂石油醚进行油脂抽提，有机溶剂与微藻的液固比（L:kg）为3：1，离心后有机相中可得到收率为92%的油脂。Add phosphoric acid with a mass percentage concentration (based on the dry weight of microalgae) of 2% to an acetic acid solution with a mass percentage concentration of 50%, and the resulting mixed solution and microalgae Botryococcus sp. have a liquid-solid ratio of 5:1 (L:kg) After mixing, stir at the boiling point of normal pressure for 4.0 hours, and then use organic solvent petroleum ether for oil extraction. The liquid-solid ratio (L:kg) of organic solvent and microalgae is 3:1. After centrifugation, it can be obtained in the organic phase Yield is 92% grease.
实施例13微藻油脂的提取方法The extraction method of embodiment 13 microalgae oil
将质量百分比浓度为60%的甲酸溶液与微藻T.pseudonana按液固比为9：1（L:kg）混合后，在常压沸点下搅拌处理2.0小时，再用有机溶剂氯仿进行油脂抽提，有机溶剂与微藻的液固比（L:kg）为6：1，离心后有机相中可得到收率为92%的油脂。Mix the formic acid solution with a mass percentage concentration of 60% and the microalgae T.pseudonana at a liquid-solid ratio of 9:1 (L:kg), stir at the boiling point of normal pressure for 2.0 hours, and then use the organic solvent chloroform for oil extraction. The liquid-solid ratio (L:kg) of the organic solvent to the microalgae was 6:1, and the oil with a yield of 92% could be obtained in the organic phase after centrifugation.
实施例14微藻油脂的提取方法The extraction method of embodiment 14 microalgae oil
将质量百分比浓度为80%的乙酸溶液与微藻T.fluviatilis按液固比为7：1（L:kg）混合后，在常压沸点下搅拌处理5.0小时，再用有机溶剂正己烷进行油脂抽提，有机溶剂与微藻的液固比（L:kg）为7：1，离心后有机相中可得到收率为93%的油脂。Mix the 80% acetic acid solution with the microalgae T.fluviatilis at a liquid-solid ratio of 7:1 (L:kg), stir at the boiling point of normal pressure for 5.0 hours, and then use the organic solvent n-hexane to degrease For extraction, the liquid-solid ratio (L:kg) of the organic solvent to the microalgae is 7:1, and the oil with a yield of 93% can be obtained in the organic phase after centrifugation.
虽然，上文中已经用一般性说明及具体实施方案对本发明作了详尽的描述，但在本发明基础上，可以对之作一些修改或改进，这对本领域技术人员而言是显而易见的。因此，在不偏离本发明精神的基础上所做的这些修改或改进，均属于本发明要求保护的范围。Although the present invention has been described in detail with general descriptions and specific embodiments above, it is obvious to those skilled in the art that some modifications or improvements can be made on the basis of the present invention. Therefore, the modifications or improvements made on the basis of not departing from the spirit of the present invention all belong to the protection scope of the present invention.
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