CN103820205A - Method for preparing highly unsaturated camellia seed clean oil by using biological enzyme method - Google Patents
Method for preparing highly unsaturated camellia seed clean oil by using biological enzyme method Download PDFInfo
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- CN103820205A CN103820205A CN201410108289.9A CN201410108289A CN103820205A CN 103820205 A CN103820205 A CN 103820205A CN 201410108289 A CN201410108289 A CN 201410108289A CN 103820205 A CN103820205 A CN 103820205A
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- 238000000034 method Methods 0.000 title claims abstract description 17
- 235000018597 common camellia Nutrition 0.000 title claims abstract description 16
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 13
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 13
- 240000001548 Camellia japonica Species 0.000 title description 9
- 229940088598 enzyme Drugs 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 241000209507 Camellia Species 0.000 claims abstract description 7
- 108010059892 Cellulase Proteins 0.000 claims abstract description 5
- 229940106157 cellulase Drugs 0.000 claims abstract description 5
- 239000008157 edible vegetable oil Substances 0.000 claims description 20
- 239000003921 oil Substances 0.000 claims description 16
- 235000019198 oils Nutrition 0.000 claims description 16
- 241001122767 Theaceae Species 0.000 claims description 15
- 238000004519 manufacturing process Methods 0.000 claims description 14
- 230000015556 catabolic process Effects 0.000 claims description 9
- 239000000839 emulsion Substances 0.000 claims description 9
- 239000007764 o/w emulsion Substances 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 5
- 108010059820 Polygalacturonase Proteins 0.000 claims description 4
- 102000035195 Peptidases Human genes 0.000 claims description 3
- 108091005804 Peptidases Proteins 0.000 claims description 3
- 239000000243 solution Substances 0.000 claims description 3
- 238000007605 air drying Methods 0.000 claims description 2
- 210000002421 cell wall Anatomy 0.000 claims description 2
- 150000001875 compounds Chemical class 0.000 claims description 2
- 238000010790 dilution Methods 0.000 claims description 2
- 239000012895 dilution Substances 0.000 claims description 2
- 230000002255 enzymatic effect Effects 0.000 claims description 2
- 241000411851 herbal medicine Species 0.000 claims description 2
- 230000007062 hydrolysis Effects 0.000 claims description 2
- 238000006460 hydrolysis reaction Methods 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 238000005303 weighing Methods 0.000 claims 2
- 239000002131 composite material Substances 0.000 claims 1
- 238000003801 milling Methods 0.000 abstract description 3
- 235000021122 unsaturated fatty acids Nutrition 0.000 abstract description 3
- 150000004670 unsaturated fatty acids Chemical class 0.000 abstract description 3
- 235000013305 food Nutrition 0.000 abstract description 2
- 108091005658 Basic proteases Proteins 0.000 abstract 1
- 239000000084 colloidal system Substances 0.000 abstract 1
- 238000001035 drying Methods 0.000 abstract 1
- 239000003960 organic solvent Substances 0.000 abstract 1
- 239000002002 slurry Substances 0.000 abstract 1
- 239000004519 grease Substances 0.000 description 5
- 239000002904 solvent Substances 0.000 description 4
- 238000005265 energy consumption Methods 0.000 description 3
- 238000010298 pulverizing process Methods 0.000 description 3
- 235000015112 vegetable and seed oil Nutrition 0.000 description 3
- 244000147058 Derris elliptica Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000009837 dry grinding Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000010792 warming Methods 0.000 description 2
- 244000060011 Cocos nucifera Species 0.000 description 1
- 235000013162 Cocos nucifera Nutrition 0.000 description 1
- 240000003133 Elaeis guineensis Species 0.000 description 1
- 235000001950 Elaeis guineensis Nutrition 0.000 description 1
- 235000019484 Rapeseed oil Nutrition 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000010779 crude oil Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 239000002360 explosive Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000002787 reinforcement Effects 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
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- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Edible Oils And Fats (AREA)
Abstract
The invention relates to a method for preparing highly unsaturated camellia seed clean oil by using a biological enzyme method, belonging to the field of food biotechnology. The method comprises the steps of hulling camellia seeds, drying, crushing, adding water, mixing, milling with a colloid mill till the granularity of slurry is 2-50mu m, performing enzymolysis wall-breaking through pectase NCB-PE40 and cellulase AE80 together, and centrifuging to separate clean oil and emulsified oil; demulsifying the emulsified oil by using alkaline protease Alcalase2.4L, and centrifuging to obtain clean oil; combining the clean oil obtained by demulsifying and the clean oil obtained by wall-breaking to finally obtain the highly unsaturated camellia seed clean oil. The method disclosed by the invention has the characteristics that the clean oil is extracted through wall-breaking and demulsifying by adopting the biological enzyme method, so that the process is simple, and the conditions are mild; the product has high unsaturated fatty acid content, does not have organic solvent residue and is environment-friendly.
Description
One, technical field
The invention provides a kind of biological enzyme and prepare the production method of high unsaturated oil tea seed edible vegetable oil, belong to technical field of food biotechnology.
Two, background technology
Oil tea is the important woody oil tree species of south China, with Fructus oleae europaeae, oil palm, coconut and be called the large woody oil tree species in the world four.China's oil tea aboundresources, cultivated area reaches 4,000,000 hectares, produces 150000 tons of camellia seed oils per year, at the forefront in the world.Camellia seed oil unsaturated fatty acid content, up to more than 85%, is rich in essential amino acid and zinc, selenium and other trace elements, is the edible oil of high-quality, is described as " treasure of oil ", has important value of exploiting and utilizing.Conventional grease preparation method is mainly milling process and solvent method.Milling process, mainly by mechanical external force, squeezes out grease from oil plant, and product is not containing solvent, but its grouts Residual oil is many, and oil yield efficiency is low, and energy consumption is large; Solvent method oil yield is high, easily realizes scale production, but solvent for use is mostly inflammable and explosive, and production security is poor, and contains dissolvent residual in processed oil, leaches crude oil more containing non-oil substances, and color and luster is darker, second-rate.The present invention adopts Biological preparation camellia seed oil, destroy the fiber reinforcement of plant cell wall by compound wall breaking enzyme, the grease of parcel is dissociated out, utilize the grease compounded body of Sumizyme MP Alcalase2.4L destruction and protein bound, further discharge free oil by breakdown of emulsion, its technique is simple, put forward oily mild condition, energy consumption is low, oil quality is high, nutrition and active function strong, the tea seed edible vegetable oil unsaturated fatty acid content of carrying reaches more than 88%, product safety, green, environmental protection, meet the demand of human consumer to nutrient health grease.
Three, summary of the invention
Technical problem the present invention has developed a kind of method that biological enzyme is prepared high unsaturated oil tea seed edible vegetable oil.Take tea seed as raw material, through arrangement, removal of impurities, shell, air-dry, pulverizing, complex enzyme hydrolysis broken wall, Sumizyme MP Alcalase2.4L enzyme breakdown of emulsion, finally obtain high unsaturated oil tea seed edible vegetable oil.
Technical scheme technical scheme and production technique are as follows:
A, break process: select tea seeds ripe, full grains, shell, put in tunnel drier forced air drying 12-24h under 40 ℃ of conditions, until its moisture content lower than 5% time, pulverize with the portable crusher for Chinese herbal medicine of FW177 type (manufacture of Tianjin Stettlen Instrument Ltd.), cross 20 mesh sieves, obtain camellia seed kernel powder;
B, enzymolysis broken wall: by camellia seed kernel powder with water with 1: 2-10 ratio is mixed, after being ground to granularity and being 2-50 μ m with JMS-50 colloidal mill (manufacture of Langfang City Guan Tong Machinery Co., Ltd.), add 0.05-10% by polygalacturonase NCB-PE40 and cellulase AE80 with the formulated prozyme of mass ratio 1: 1-3, under temperature 40-60 ℃ and pH4-6 condition, enzymolysis 1-6h; By broken wall enzymolysis solution, under 30-60 ℃ and 2000-5000rpm condition, centrifugal 10-15min, obtains edible vegetable oil, oil in water emulsion, water layer and residue;
C, proteolytic enzyme breakdown of emulsion: get oil in water emulsion, add water with 1: 1-10 dilution proportion, under pH8-12 and temperature 50-80 ℃ condition, stir after 40-60min, add 0.1-10% Sumizyme MP Alcalase2.4L, under 60-80 ℃ of condition, control pH8-12, enzymolysis 1-4h;
D, edible vegetable oil separate: by the enzymolysis solution after breakdown of emulsion, under 50-80 ℃ and 2000-5000rpm condition, centrifugal 10-15min, obtains edible vegetable oil, and the edible vegetable oil during with enzymatic shell-broken merges.
The present invention compared with prior art, has following characteristics and beneficial effect:
1, adopt biological enzyme processing, processing unit is simple, operational safety, and energy consumption is low, pollution is few;
2, extract tea seed edible vegetable oil by prozyme broken wall and proteolytic enzyme breakdown of emulsion, gentleer, can effectively retain nutrition and the active function of rapeseed oil;
3, product organic solvent-free is residual, and production security is good, environmental protection.
Four, embodiment
Embodiment 1
Get the 1kg shelling portable medicinal herb grinder of camellia seed kernel FW177 type (manufacture of Tianjin Stettlen Instrument Ltd.) and carry out dry milling, camellia seed kernel after pulverizing and water are mixed with the ratio of 1: 5, being ground to its granularity with JMS-50 colloidal mill (manufacture of Langfang City Guan Tong Machinery Co., Ltd.) is 2-50 μ m, add prozyme (4g polygalacturonase NCB-PE40,6g cellulase AE80), under 50 ℃ and pH4.5 condition, enzymolysis 3h, then under 40 ℃ and 3000rpm condition, centrifugal 10min.Get oil in water emulsion, in the ratio distilled water diluting of 1: 1, regulate pH9, be warming up to 60 ℃, keep 40min, add 2.5ml Sumizyme MP Alcalase2.4L, keep 60 ℃, in pH9 left and right, enzymolysis 60min, under 50 ℃ and 3500rpm condition, centrifugal 10min, obtain tea seed edible vegetable oil, the edible vegetable oil obtaining during with broken wall merges.
Embodiment 2
Get the 1kg shelling portable medicinal herb grinder of camellia seed kernel FW177 type (manufacture of Tianjin Stettlen Instrument Ltd.) and carry out dry milling, camellia seed kernel after pulverizing and water are mixed with the ratio of 1: 6, being ground to its granularity with JMS-50 colloidal mill (manufacture of Langfang City Guan Tong Machinery Co., Ltd.) is 2-50 μ m, add prozyme (5g polygalacturonase NCB-PE40,15g cellulase AE80), under 55 ℃ and pH5 condition, enzymolysis 4h, then under 40 ℃ and 3500rpm condition, centrifugal 15min.Get oil in water emulsion, in the ratio distilled water diluting of 1: 2, regulate pH11, be warming up to 65 ℃, keep 60min, add 5ml Sumizyme MP Alcalase2.4L, keep 65 ℃, in pH11 left and right, enzymolysis 100min, under 60 ℃ and 3500rpm condition, centrifugal 15min, obtain tea seed edible vegetable oil, the edible vegetable oil obtaining with broken wall merges.
Claims (3)
1. biological enzyme is prepared a method for high unsaturated oil tea seed edible vegetable oil, it is characterized in that taking following technique:
A, break process: select tea seeds ripe, full grains, shell, put in tunnel drier forced air drying 12-24h under 40 ℃ of conditions, until moisture content lower than 5% time, pulverize with the portable crusher for Chinese herbal medicine of FW177 type (manufacture of Tianjin Stettlen Instrument Ltd.), cross 20 mesh sieves, obtain camellia seed kernel powder;
B, enzymolysis broken wall: by camellia seed kernel powder with water with 1: 2-10 ratio is mixed, after being ground to granularity and being 2-50 μ m with JMS-50 colloidal mill (manufacture of Langfang City Guan Tong Machinery Co., Ltd.), add complex enzyme zymohydrolysis tea seed cell walls, then under 30-60 ℃ and 2000-5000rpm condition, centrifugal 10-15min, obtains edible vegetable oil, oil in water emulsion, water layer and residue;
C, proteolytic enzyme breakdown of emulsion: get oil in water emulsion, add water with 1: 1-10 dilution proportion, under pH8-12 and temperature 50-80 ℃ condition, stirs after 40-60min, then adds Sumizyme MP Alcalase2.4L to carry out enzymolysis breakdown of emulsion;
D, edible vegetable oil separate: by the enzymolysis solution after breakdown of emulsion, under 50-80 ℃ and 2000-5000rpm condition, centrifugal 10-15min, obtains edible vegetable oil, and the edible vegetable oil during with enzymatic shell-broken merges.
2. according to claim 1, it is characterized in that: described compound wall breaking enzyme is formed so that mass ratio 1: 1-3 is composite by polygalacturonase NCB-PE40 and cellulase AE80, and addition is 0.05-10%, hydrolysis temperature 40-60 ℃, enzymolysis 1-6h, enzymolysis pH4-6; Described addition is with camellia seed kernel powder weighing scale.
3. according to claim 1, it is characterized in that: when oil in water emulsion breakdown of emulsion, add 0.1-10% Sumizyme MP Alcalase2.4L, under 60-80 ℃ of condition, control pH8-12, enzymolysis 1-4h; Described addition is with oil in water emulsion weighing scale.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104152260A (en) * | 2014-09-08 | 2014-11-19 | 南京飞马食品有限公司 | Biological extraction method of high-unsaturated-activity salicornia bigelovii seed oil |
| CN104152271A (en) * | 2014-09-08 | 2014-11-19 | 南京飞马食品有限公司 | Method for assisting and extracting lavandula essential oil by enzyme method |
| CN104928007A (en) * | 2015-06-02 | 2015-09-23 | 南京飞马食品有限公司 | Production method for extraction of mulberry seed edible vegetable oil with aqueous enzymatic method |
| CN104928008A (en) * | 2015-06-10 | 2015-09-23 | 南京飞马食品有限公司 | Method for producing active lycopersicum esculentum Mill seed edible vegetable oil by adopting biological enzyme method |
| CN104928006A (en) * | 2015-06-02 | 2015-09-23 | 南京飞马食品有限公司 | Biological preparation method for active perilla frutescens seed edible vegetable oil |
| CN104928012A (en) * | 2015-05-26 | 2015-09-23 | 南昌大学 | Method for extracting tea oil by demulsification based on aqueous enzymatic method |
| CN104946378A (en) * | 2015-06-02 | 2015-09-30 | 南京飞马食品有限公司 | Bio-extraction method of carthamus tinctorius seed clear oil |
| CN104946379A (en) * | 2015-06-02 | 2015-09-30 | 南京飞马食品有限公司 | Production method of active carthamus tinctorius clear oil |
| CN106260354A (en) * | 2016-08-22 | 2017-01-04 | 穆毅 | A kind of Mount Taibai medicine Fructus Armeniacae Mume leaf alternative tea |
| CN109370763A (en) * | 2018-12-27 | 2019-02-22 | 东北林业大学 | A kind of preparation method of peony seed oil |
| CN118516173A (en) * | 2024-05-16 | 2024-08-20 | 湖南省林业科学院 | Preparation method and application of water-soluble camellia oil |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104152260A (en) * | 2014-09-08 | 2014-11-19 | 南京飞马食品有限公司 | Biological extraction method of high-unsaturated-activity salicornia bigelovii seed oil |
| CN104152271A (en) * | 2014-09-08 | 2014-11-19 | 南京飞马食品有限公司 | Method for assisting and extracting lavandula essential oil by enzyme method |
| CN104928012A (en) * | 2015-05-26 | 2015-09-23 | 南昌大学 | Method for extracting tea oil by demulsification based on aqueous enzymatic method |
| CN104928007A (en) * | 2015-06-02 | 2015-09-23 | 南京飞马食品有限公司 | Production method for extraction of mulberry seed edible vegetable oil with aqueous enzymatic method |
| CN104928006A (en) * | 2015-06-02 | 2015-09-23 | 南京飞马食品有限公司 | Biological preparation method for active perilla frutescens seed edible vegetable oil |
| CN104946378A (en) * | 2015-06-02 | 2015-09-30 | 南京飞马食品有限公司 | Bio-extraction method of carthamus tinctorius seed clear oil |
| CN104946379A (en) * | 2015-06-02 | 2015-09-30 | 南京飞马食品有限公司 | Production method of active carthamus tinctorius clear oil |
| CN104928008A (en) * | 2015-06-10 | 2015-09-23 | 南京飞马食品有限公司 | Method for producing active lycopersicum esculentum Mill seed edible vegetable oil by adopting biological enzyme method |
| CN106260354A (en) * | 2016-08-22 | 2017-01-04 | 穆毅 | A kind of Mount Taibai medicine Fructus Armeniacae Mume leaf alternative tea |
| CN109370763A (en) * | 2018-12-27 | 2019-02-22 | 东北林业大学 | A kind of preparation method of peony seed oil |
| CN118516173A (en) * | 2024-05-16 | 2024-08-20 | 湖南省林业科学院 | Preparation method and application of water-soluble camellia oil |
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Application publication date: 20140528 |