CN104928007A

CN104928007A - Production method for extraction of mulberry seed edible vegetable oil with aqueous enzymatic method

Info

Publication number: CN104928007A
Application number: CN201510292075.6A
Authority: CN
Inventors: 李春阳; 李世科; 张红城
Original assignee: NANJING FEIMA FOOD Co Ltd
Current assignee: NANJING FEIMA FOOD Co Ltd
Priority date: 2015-06-02
Filing date: 2015-06-02
Publication date: 2015-09-23

Abstract

The invention discloses a method for extraction of mulberry seed edible vegetable oil with an aqueous enzymatic method, and belongs to the technical field of food biotechnology. The method comprises the following steps: mulberry seeds are cleaned, purified with dust removed, dried, crushed and added with water to be mixed, and then, the mixture is compounded with pectinase NCB-PE40 and xylanase NCB-X50 to be subjected to enzymolysis and wall-breaking; besides, an appropriate amount of alkaline protease Alcalase2.4L is added for enzymolysis, centrifugation is performed, and the supernatant liquid is obtained, therefore, the high-unsaturated active mulberry seed edible vegetable oil is obtained. The production method has the characteristics that a biological enzyme method is adopted for extracting oil, the process is simple, the condition is mild, and the obtained mulberry seed edible vegetable oil is high in unsaturated fatty acid content, strong in active function, free of residual organic solvents, and green and environment-friendly.

Description

A kind of production method of aqueous enzymatic extraction mulberry fruit seed edible vegetable oil

Technical field

The invention provides a kind of method of aqueous enzymatic extraction mulberry fruit seed edible vegetable oil, belong to technical field of food biotechnology.

Background technology

Mulberry fruit ( mulberry) be commonly called as mulberry jujube, sorosis, for moraceae plants mulberry ( morus alba L.) dry fruit ear, its nutrition be the 5-6 of apple doubly, be described as " the best health fruit of 21 century " by medical circle.Because the growing environment that mulberry tree is special makes sorosis have spontaneous growth, without the feature of any pollution, so mulberry fruit is otherwise known as " holy fruit among the people ".China's mulberry fruit distribution is wide, mulberry fruit aboundresources.At present, China mainly concentrates on the aspect of squeezing the juice of mulberry fruit to the exploitation of mulberry fruit, and pomace after squeezing the juice, seed are discarded, and fully do not develop.

Ripe fresh mulberry fruit is containing seed about 2.8%, and in mulberry fruit seed fatty about 30.7%, its unsaturated fatty acid content is higher, and mulberry seed oil has reducing blood-fat, study of anti-atherogenic effect, can delay body aging, has good economic development value.Therefore, mulberry seed oil is a kind of colory new type of health edible vegetable oil.

Conventional grease preparation method is mainly milling process and solvent method.Grease, mainly by mechanical external force, squeezes out by milling process from oil plant, and product is not containing solvent, but its grouts Residual oil is many, and oil yield efficiency is low, and energy consumption is large; Solvent method oil yield is high, easily realizes scale production, but solvent for use is mostly inflammable and explosive, and production security is poor, and containing dissolvent residual in processed oil, Extractive crudeoil is more containing non-oil substances, and color and luster is comparatively dark, second-rate.Aqueous enzymatic method carries oil tech as one is novel, biological enzymolysis is utilized to destroy the fiber reinforcement of plant cell wall, the grease of parcel is dissociated out, also can destroy the grease compounded body with carbohydrate or protein bound simultaneously, discharge grease, its technique be simple, put forward oily mild condition, energy consumption is low, oil quality is high, nutrition and active function strong.

The present invention adopts aqueous enzymatic method to prepare mulberry seed oil, and by the grease in compound wall breaking enzyme release mulberry fruit seed, then use Sumizyme MP Alcalase2.4L enzymolysis, obtain unsaturated fatty acid content up to 85%, linoleic acid content can reach the active mulberry fruit seed edible vegetable oil of 74%.Present method is simple and easy to do, green, environmental protection, energy-conservation, and production cost is low, safe and reliable, meets the demand of human consumer to nutritive health-care grease.

Summary of the invention

Technical problem this invention exploits a kind of method of aqueous enzymatic extraction mulberry fruit seed edible vegetable oil.With mulberry fruit seed for raw material, through arrangement, removal of impurities, air-dry, pulverizing, enzymolysis broken wall, proteolysis, centrifugation, obtain high unsaturated active mulberry fruit seed edible vegetable oil;

Technical scheme technical scheme and production technique as follows:

1, break process: mulberry fruit seed is after cleaning, removal of impurities, be placed in tunnel drier forced air drying 12-24h under 20-60 DEG C of condition, until its moisture content lower than 5% time, pulverize with the portable crusher for Chinese herbal medicine of FW177 type (manufacture of Tianjin Stettlen Instrument Ltd.), cross 40-100 mesh sieve;

2, broken wall enzymolysis: get after the mulberry fruit seed powder after a certain amount of pulverizing mixes with 1:5-10 ratio with water, add 0.05-10% prozyme (composite with mass ratio 1:1-7 by polygalacturonase NCB-PE40 and zytase NCB-X50), under 40-60 DEG C and pH4-6 condition, enzymolysis 1-6h;

3, proteolysis: by enzymolysis solution under pH8-12 and 50-80 DEG C of condition, stirs 40-60min, then adds 0.1-10% Sumizyme MP Alcalase2.4L, under pH8-12 and 60-80 DEG C of condition, and enzymolysis 1-4h; Described addition is with mulberry seeds weighing scale;

4, edible vegetable oil is separated: by enzymolysis solution under 50-80 DEG C and 2000-5000rpm condition, centrifugal 10-15min, obtains high unsaturated active mulberry seed edible vegetable oil.

The present invention compared with prior art, has following characteristics and beneficial effect:

1, adopt biological enzyme process, processing unit is simple, operational safety, and energy consumption is low, pollution is few;

2, by prozyme broken wall and proteolysis, extract mulberry seed edible vegetable oil, product quality be high, nutrition and active function strong;

3, the active mulberry fruit seed edible vegetable oil produced, organic solvent-free remains, and production security is good, environmental protection.

Embodiment

Below in conjunction with specific embodiment, the invention will be further described:

embodiment 1

Get 50g mulberry fruit seed powder, mix with 250ml water, add compound wall breaking enzyme (0.5g polygalacturonase NCB-PE40,2.5g zytase NCB-X50), under 40 DEG C and pH4.8 condition, enzymolysis 1h, then regulates about pH9, be warming up to 60 DEG C, keep 40min, add 2.5ml Sumizyme MP Alcalase2.4L, keep 60 DEG C and pH value about 9, enzymolysis 60min, under 50 DEG C and 3500rpm condition, centrifugal 10min, obtained high unsaturated active mulberry seed edible vegetable oil.

embodiment 2

Get 50g mulberry fruit seed powder, mix with 500ml water, add compound wall breaking enzyme (1g polygalacturonase NCB-PE40,2g zytase NCB-X50), under 50 DEG C and pH4.5 condition, enzymolysis 3h, then regulates about pH10, be warming up to 60 DEG C, keep 40min, add 1ml Sumizyme MP Alcalase2.4L, keep 80 DEG C and pH value about 10, enzymolysis 80min, under 50 DEG C and 3500rpm condition, centrifugal 10min, obtained high unsaturated active mulberry fruit seed edible vegetable oil.

Claims

1. a method for aqueous enzymatic extraction mulberry fruit seed edible vegetable oil, is characterized in that taking following steps:

A, break process: mulberry fruit seed is after arranging removal of impurities, be placed in tunnel drier forced air drying 12-24h under 20-60 DEG C of condition, until moisture content lower than 5% time, pulverize with the portable crusher for Chinese herbal medicine of FW177 type (manufacture of Tianjin Stettlen Instrument Ltd.), cross 40-100 mesh sieve;

B, broken wall enzymolysis: get a certain amount of mulberry fruit seed powder and mix with certain proportion with water, add 0.05-10% prozyme, under 40-60 DEG C and pH4-6 condition, enzymolysis 1-6h;

C, proteolysis: by broken wall enzymolysis solution, under pH8-12 and 50-80 DEG C of condition, stir 40-60min, then add 0.1-10% Sumizyme MP Alcalase2.4L, carry out stirring enzymolysis;

D, edible vegetable oil are separated: by enzymolysis solution under 50-80 DEG C and 2000-5000rpm condition, and centrifugal 10-15min is separated and obtains mulberry fruit seed edible vegetable oil.

2. the method for a kind of aqueous enzymatic extraction mulberry fruit seed edible vegetable oil according to claim 1, it is characterized in that in step B, mulberry fruit seed powder mixes with 1:5-10 ratio with water, add by polygalacturonase NCB-PE40 and zytase NCB-X50 with the composite wall breaking enzyme of mass ratio 1:1-7; Described enzyme addition is with mulberry fruit seed opaque gauge.