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CN102870720A - Low-salinity outdoor enclosure type epinephelus seedling method - Google Patents

Low-salinity outdoor enclosure type epinephelus seedling method Download PDF

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Publication number
CN102870720A
CN102870720A CN2012104210141A CN201210421014A CN102870720A CN 102870720 A CN102870720 A CN 102870720A CN 2012104210141 A CN2012104210141 A CN 2012104210141A CN 201210421014 A CN201210421014 A CN 201210421014A CN 102870720 A CN102870720 A CN 102870720A
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China
Prior art keywords
enclosure
salinity
grouper
low
water
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CN2012104210141A
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Inventor
陈刚
汤保贵
施钢
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Guangdong Ocean University
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Guangdong Ocean University
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Priority to CN2012104210141A priority Critical patent/CN102870720A/en
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Farming Of Fish And Shellfish (AREA)

Abstract

本发明公开了一种石斑鱼类低盐度室外围隔式育苗方法。其包括以下步骤:清池消毒:选择育苗池,消毒后引入海水;肥水培育:向育苗池的水体中施入农用复合有机肥,培育肥水至透明度25~30cm;接种轮虫;围隔准备:在育苗池中设置若干个围隔,用人工海盐将围隔中的独立水体调至盐度为30‰~35‰;受精卵孵化:将受精卵置于围隔中进行孵化;围隔打开:仔鱼孵出3天后,将围隔打开一个间隙,使围隔内外进行水流自然交换,内外盐度逐步达到平衡,仔鱼也可以通过间隙到达围隔外;接种桡足类;接种丰年虫;育苗完成。本发明在育苗池中进行围隔式加盐孵化,将孵化、开口、仔稚鱼培育在同池完成,最大限度减少操作与环境应激,从而提高育苗成活率。The invention discloses a method for cultivating grouper fish with low-salinity outdoor peripheral partition type. It includes the following steps: cleaning pond disinfection: selecting seedling ponds, introducing seawater after disinfection; fertilizer and water cultivation: applying agricultural compound organic fertilizer to the water body of seedling ponds, cultivating fertilizer water to a transparency of 25-30 cm; inoculating rotifers; enclosure preparation: Set several enclosures in the nursery pool, and use artificial sea salt to adjust the independent water body in the enclosure to a salinity of 30‰~35‰; fertilized egg hatching: place the fertilized eggs in the enclosure for incubation; open the enclosure: Three days after the larvae hatched, open a gap in the enclosure to allow natural exchange of water flow between the inside and outside of the enclosure, and the internal and external salinity gradually reached a balance, and the larvae can also reach outside the enclosure through the gap; inoculate copepods; . The present invention carries out enclosed salt hatching in the seedling pond, completes hatching, opening, and larval fish cultivation in the same pond, and minimizes operation and environmental stress, thereby improving the survival rate of seedlings.

Description

The outdoor enclosure formula of ablen Low-salinity seedling-cultivating method
Technical field
The present invention relates to a kind of fry rearing method, specifically refer to the outdoor enclosure formula of a kind of ablen Low-salinity seedling-cultivating method.
Background technology
Under field conditions (factors), ablen is fattened in the marine site, off-lying sea usually, gonad maturity, lay eggs, fertilization, embryo and son, young, Larval Development, the there condition of water quality is superior, salinity is high and stable, extremely is conducive to the early development of fish.
At present, although ablen has realized that the total man worker of seedling breeds, the not high industrial circle that always perplexing of the success rate of growing seedlings, one of them very crucial factor is exactly salinity.Marine Fish During The Artificial Seedling carries out at offshore usually, owing to being subject to the impact of rainfall and rainwash, the offshore salinity is usually less than the off-lying sea.Especially 6~September is again multiple season of typhoon, rainfall mating season of grouper at a time when this, and the coastal seawater salinity is usually low by about 5~10 ‰ than the off-lying sea, and is very unfavorable for the grouper artificial breeding.
In order to satisfy the stage of growing seedlings to the demand of salinity, industrial circle generally takes salt adding in early stage, middle and later periods to move the multistage pattern of growing seedlings of pond salt reduction.But also there is obvious defective in this pattern: (1) multistage moves that pond labour intensity is large, operation stress be strong, and the fry loss is large; The differences such as the water temperature that (2) exists between different nursery ponds, salinity, current, small seedling are very easy to produce stress reaction and death; (3) multistage pond seedling multiple-contact transplant seedlings fishing gear, the very easily pathogenic infection of moving; (4) full water body salt adding lifting salinity is with high costs, seldom adopts in the production.
Above-mentioned discussion content purpose is to introduce to the reader various aspects of technology that may be relevant with the various aspects of the present invention that below will be described and/or advocate, believe that this discussion content helps to provide background information for the reader, to be conducive to understanding better various aspects of the present invention, therefore, should be appreciated that it is to read these discussions with this angle, rather than admit prior art.
Summary of the invention
The object of the invention is to avoid deficiency of the prior art and the outdoor enclosure formula of a kind of ablen Low-salinity seedling-cultivating method is provided, it carries out the hatching of enclosure formula salt adding in nursery pond, hatching, opening, larval rearing are finished at Tong Chi, reduce to greatest extent operation and environmental stress, thereby improve survival rate of seedling.
Purpose of the present invention is achieved through the following technical solutions:
The outdoor enclosure formula of a kind of ablen Low-salinity seedling-cultivating method is provided, may further comprise the steps:
Clear pond sterilization: select nursery pond, introduce seawater after the sterilization;
Rich water is cultivated: apply agricultural compound organic fertilizer in the water body of nursery pond, cultivate rich water to transparency 25~30cm;
Inoculating rotifer;
Enclosure is prepared: several enclosures are set in nursery pond, and with artificial sea salt the independent water body in the enclosure being transferred to salinity is 30 ‰~35 ‰;
Incubating oosperm: place enclosure to hatch fertilized egg;
Enclosure is opened: after prelarva hatches 3 days, enclosure is opened a gap, make and carry out current inside and outside the enclosure and naturally exchange, inside and outside salinity progressively reaches balance, and prelarva also can arrive outside the enclosure by the gap;
The inoculation copepoda;
The inoculation fairy shrimp;
Grow seedlings and finish.
Wherein, after inoculation fairy shrimp step, also comprise the water body step of topdressing: change and the food density rational application of fertilizer according to water transparency, keep water transparency about 30~35cm.
Wherein, in the sterilisation step of clear pond: with the bleaching powder sterilization, consumption is 5~10 Kg/ mus; Add clean seawater to 0.8~1.5m after 7 days.
Wherein, in the rich water incubation step: in water body, apply agricultural compound organic fertilizer by 15~20 Kg/ mu consumptions.
Wherein, in the inoculating rotifer step: by 0.5~1.5Kg/ mu consumption inoculating rotifer, as the open-mouthed bait of newly hatched larvae.
Wherein, in the enclosure preparation process: described enclosure covers sunshade net, and sunshade net makes the interior intensity of illumination of enclosure not be higher than 5500 luxs.
Wherein, in the enclosure preparation process: arrange that in the enclosure water body 15~20 gas stones inflate, make water body be the boiling shape.
Wherein, in the incubating oosperm step: cloth ovum density is no more than 300g/ m 3, turn simultaneously the aeration quantity of gas stone down, make the water surface be corrugated, thereby ovum is slightly rolled at the water surface.
Wherein, in inoculation copepoda step: after prelarva hatches rear 10 days, in nursery pond, access copepoda by 0.5~1.5Kg/ mu consumption, ingest for prelarva.
Wherein, in inoculation fairy shrimp step: after prelarva hatches rear 18 days, begin to hatch fairy shrimp and throw something and feed, the initial stage, the later stage suitably was increased to 600~800g/, ten thousand tails by per ten thousand tail fry hatchings, 400~600g brine shrimp eggs.
Beneficial effect of the present invention: the present invention adopts " three grades " enclosure formula seedling growing process, in the Low-salinity zone or carry out Marine Fish During The Artificial Seedling season.Enclosure is set in nursery pond, the salt adding hatching, along with fry behind opening, the adaptive capacity that salinity is required progressively to reduce and salinity is changed strengthens gradually, and enclosure is opened, can enlarge on the one hand the seedling water space, reduce seedling density, simultaneously, naturally spread by salinity, slowly reduce water salinity, reduce operation and environmental stress, thereby improve survival rate of seedling.
Embodiment
In order to make those skilled in the art understand better technical scheme of the present invention, the present invention is described in further detail below in conjunction with the drawings and specific embodiments, need to prove, in the situation that do not conflict, the application's embodiment and the feature among the embodiment can make up mutually.
Core of the present invention is to provide the outdoor enclosure formula of a kind of ablen Low-salinity seedling-cultivating method, it carries out the hatching of enclosure formula salt adding in nursery pond, hatching, opening, larval rearing are finished at Tong Chi, reduced to greatest extent operation and environmental stress, thereby improve survival rate of seedling.
The outdoor enclosure formula of ablen Low-salinity of the present invention seedling-cultivating method specifically may further comprise the steps:
1, clear pond sterilization: select nursery pond, nursery pond can be outdoor tank-culture or the high-order pond of plastic film mulch, and 1~3 mu of area is advisable, and with the bleaching powder sterilization, the bleaching powder consumption is 5~10 Kg/ mus; Add clean seawater after 7 days to 1.2m, intake with 300 order net filtrations.
2, rich water is cultivated: apply agricultural compound organic fertilizer by 15~20 Kg/ mu consumptions in water body, cultivate rich water to transparency 25~30cm.
3, inoculating rotifer: pressed afterwards 1Kg/ mu consumption inoculating rotifer in 3 days, as the open-mouthed bait of newly hatched larvae.
4, enclosure is prepared: several enclosures are set in nursery pond, make rectangle or square buoyancy aid framework about 3m * 4m with the 110mmPVC pipe, again with colour bar cloth bind on framework, make 10~12m 3Enclosure, the outer water body of the water body in the enclosure and enclosure is separate.Cover the black sunshade net on the enclosure, illumination intensity is not higher than 5500 luxs when requiring high noon; With artificial sea salt the independent water body in the enclosure is transferred to 30 ‰~35 ‰, and arrange that in the enclosure water body 15~20 gas stones inflate, be the boiling shape.
5, incubating oosperm; Place enclosure to hatch fertilized egg, cloth ovum density is no more than 300g/ m 3, suitably turn gas stone aeration quantity down simultaneously, make water surface be corrugated, ovum is advisable in the slight rolling of the water surface just.
6, enclosure is opened: (usually hatched rear 3 days) behind the prelarva opening, enclosure is opened a wide approximately gap of 1 meter, make and carry out current inside and outside the enclosure and naturally exchange, inside and outside salinity progressively reaches balance, and prelarva also can arrive outside the enclosure by the gap.
7, inoculation copepoda: behind the prelarva opening 7 days (hatching rear 10 days), in nursery pond, access copepoda by 1Kg/ mu consumption, ingest for prelarva.
8, inoculation fairy shrimp: about 15 days (hatching rear 18 days), begin to hatch fairy shrimp and throw something and feed behind the prelarva opening, the initial stage, the later stage suitably was increased to 600g/ ten thousand tails by per ten thousand tail fry hatching 500g brine shrimp eggs.
9, water body topdresses: change and the food density rational application of fertilizer according to water transparency, keep water transparency about 30~35cm.
10, grow seedlings and finish.
The present invention is in experimentation, successively use in growing seedlings in Epinephelus coioides, the brown some kind such as grouper, the result shows: educate to the survival rate of 3cm fry and reach respectively 31%, 29%, improved respectively 13% and 9%, the effect highly significant than the multistage pond of the routine pattern survival rate of growing seedlings.
Set forth a lot of details so that fully understand the present invention in the top description, still, the present invention can also adopt other to be different from other modes described here and implement, and therefore, can not be interpreted as limiting the scope of the invention.
In a word; although the present invention has exemplified above-mentioned preferred embodiment, should illustrate, although those skilled in the art can carry out various variations and remodeling; unless such variation and remodeling have departed from scope of the present invention, otherwise all should be included in protection scope of the present invention.

Claims (10)

1.一种石斑鱼类低盐度室外围隔式育苗方法,其特征在于,包括以下步骤: 1. a method for growing grouper low-salinity outdoor peripheral compartment seedlings, is characterized in that, comprises the following steps: 清池消毒:选择育苗池,消毒后引入海水; Cleaning pool disinfection: select the nursery pool, and introduce seawater after disinfection; 肥水培育:向育苗池的水体中施入农用复合有机肥,培育肥水至透明度25~30cm; Fertilizer and water cultivation: apply agricultural compound organic fertilizer to the water body of the seedling pond, and cultivate the fertilizer and water to a transparency of 25-30cm; 接种轮虫; Inoculation of rotifers; 围隔准备:在育苗池中设置若干个围隔,用人工海盐将围隔中的独立水体调至盐度为30‰~35‰; Enclosure preparation: set several enclosures in the nursery pond, and use artificial sea salt to adjust the independent water body in the enclosure to a salinity of 30‰~35‰; 受精卵孵化:将受精卵置于围隔中进行孵化; Incubation of fertilized eggs: placing fertilized eggs in enclosures for incubation; 围隔打开:仔鱼孵出3天后,将围隔打开一个间隙,使围隔内外进行水流自然交换,内外盐度逐步达到平衡,仔鱼也可以通过间隙到达围隔外; Open the enclosure: 3 days after the larvae hatch, open a gap in the enclosure to allow natural exchange of water flow between the inside and outside of the enclosure, the internal and external salinity gradually reaches a balance, and the larvae can also reach the outside of the enclosure through the gap; 接种桡足类; Inoculation of copepods; 接种丰年虫; inoculation with Artemia; 育苗完成。 Seedlings are completed. 2.根据权利要求1所述的石斑鱼类低盐度室外围隔式育苗方法,其特征在于,在接种丰年虫步骤后,还包括水体追肥步骤:根据水体透明度变化及饵料密度合理施肥,保持水体透明度在30~35cm左右。 2. the method for raising grouper low-salinity outdoor peripheral compartment seedlings according to claim 1, is characterized in that, after inoculating Artemia step, also comprises water body topdressing step: according to water body transparency change and bait density rational fertilization, Keep the transparency of the water body at around 30-35cm. 3.根据权利要求1所述的石斑鱼类低盐度室外围隔式育苗方法,其特征在于,在清池消毒步骤中:用漂白粉消毒,用量为5~10 Kg/亩;7天后加洁净海水至0.8~1.5m。 3. The method for growing grouper low-salinity outdoor peripheral compartment seedlings according to claim 1, characterized in that, in the cleaning pond disinfection step: use bleaching powder for disinfection, and the dosage is 5-10 Kg/mu; add after 7 days Clean seawater to 0.8 ~ 1.5m. 4.根据权利要求1所述的石斑鱼类低盐度室外围隔式育苗方法,其特征在于,在肥水培育步骤中:按15~20 Kg/亩用量向水体中施入农用复合有机肥。 4. the grouper low-salinity outdoor peripheral partitioned seedling raising method according to claim 1 is characterized in that, in the fertilization and water cultivation step: apply agricultural compound organic fertilizer in the water body by 15~20 Kg/mu consumption . 5.根据权利要求1所述的石斑鱼类低盐度室外围隔式育苗方法,其特征在于,在接种轮虫步骤中:按0.5~1.5Kg/亩用量接种轮虫,作为初孵仔鱼的开口饵料。 5. The method for growing grouper low-salinity outdoor peripheral compartment seedlings according to claim 1, characterized in that, in the step of inoculating rotifers: inoculating rotifers at a dosage of 0.5 to 1.5Kg/mu as newly hatched larvae open bait. 6.根据权利要求1所述的石斑鱼类低盐度室外围隔式育苗方法,其特征在于,在围隔准备步骤中:所述围隔上覆遮阳网,遮阳网使围隔内光照强度不高于5500勒克斯。 6. the grouper low-salinity outdoor enclosure seedling raising method according to claim 1, is characterized in that, in the enclosure preparation step: the enclosure is covered with a sunshade net, and the sunshade net makes the enclosure illuminated. The intensity is not higher than 5500 lux. 7.根据权利要求6所述的石斑鱼类低盐度室外围隔式育苗方法,其特征在于,在围隔准备步骤中:在围隔水体中布置15~20条气石进行充气,使水体呈沸腾状。 7. The method for growing grouper low-salinity outdoor enclosure seedlings of grouper according to claim 6, characterized in that, in the enclosure preparation step: arrange 15 to 20 air stones in the enclosure water body to inflate, so that The body of water is boiling. 8.根据权利要求7所述的石斑鱼类低盐度室外围隔式育苗方法,其特征在于,在受精卵孵化步骤中:布卵密度不超过300g/ m3,同时调小气石的充气量,使水面呈波纹状,从而使卵在水面轻微翻滚。 8. The method for raising grouper low-salinity outdoor peripheral compartment seedlings according to claim 7, characterized in that, in the hatching step of fertilized eggs: the density of laying eggs is no more than 300g/m 3 , and at the same time, the aeration of the airstone is reduced. amount so that the water surface is rippling, so that the eggs roll slightly on the water surface. 9.根据权利要求1至7中任一项所述的石斑鱼类低盐度室外围隔式育苗方法,其特征在于,在接种桡足类步骤中:仔鱼孵出后10天后,按0.5~1.5Kg/亩用量向育苗池中接入桡足类,供仔鱼摄食。 9. according to any one of claim 1 to 7, the grouper low-salinity outdoor peripheral compartment culture method is characterized in that, in the step of inoculating copepods: after 10 days after the larvae hatched, press 0.5 ~1.5Kg/acre is used to insert copepods into the nursery ponds for the larvae to feed on. 10.根据权利要求1至7中任一项所述的石斑鱼类低盐度室外围隔式育苗方法,其特征在于,在接种丰年虫步骤中:仔鱼孵出后18天后,开始孵化丰年虫投喂,初期按每万尾鱼苗孵化400~600g丰年虫卵,后期适当增加到600~800g/万尾。 10. according to any one of claims 1 to 7, the method for growing grouper low-salinity outdoor peripheral compartments, is characterized in that, in the step of inoculating Artemia worms: after 18 days after the hatching of the larvae, start to hatch Artemia Insect feeding, 400-600g Artemia worm eggs per 10,000 fry hatch at the initial stage, and increase to 600-800g/10,000 fry appropriately in the later stage.
CN2012104210141A 2012-10-30 2012-10-30 Low-salinity outdoor enclosure type epinephelus seedling method Pending CN102870720A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103210873A (en) * 2013-05-10 2013-07-24 中国水产科学研究院黑龙江水产研究所 Pond field experimentation device and using method thereof
CN112119949A (en) * 2020-09-28 2020-12-25 云南省渔业科学研究院 Method for improving survival rate of schizothorax photorrhiza in pond culture

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1444848A (en) * 2003-01-27 2003-10-01 广东省大亚湾水产试验中心 Industrial breeding method for bevel-band rockfish
CN101375673A (en) * 2007-08-27 2009-03-04 海南大学 Industrialized artificial seedling cultivation method for rockfishes
CN102657127A (en) * 2012-05-28 2012-09-12 海南省水产研究所 Facility fry-rearing method for grouper in large water body of outdoor cement pool

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1444848A (en) * 2003-01-27 2003-10-01 广东省大亚湾水产试验中心 Industrial breeding method for bevel-band rockfish
CN101375673A (en) * 2007-08-27 2009-03-04 海南大学 Industrialized artificial seedling cultivation method for rockfishes
CN102657127A (en) * 2012-05-28 2012-09-12 海南省水产研究所 Facility fry-rearing method for grouper in large water body of outdoor cement pool

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
王涵生等: "盐度对赤点石斑鱼受精卵发育的影响及仔鱼活力的判断", 《水产学报》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103210873A (en) * 2013-05-10 2013-07-24 中国水产科学研究院黑龙江水产研究所 Pond field experimentation device and using method thereof
CN112119949A (en) * 2020-09-28 2020-12-25 云南省渔业科学研究院 Method for improving survival rate of schizothorax photorrhiza in pond culture

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Application publication date: 20130116