CN104255623B - The artificial raise seedling method of a kind of ripple lip fish - Google Patents
The artificial raise seedling method of a kind of ripple lip fish Download PDFInfo
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Abstract
本发明公开了一种波纹唇鱼的人工育苗方法,包括以下步骤:A、育苗池塘的选取;B、育苗池塘的消毒;C、育苗池塘的灌水;D、培养浮游生物;E、设置孵化箱;F、受精卵的孵化;G、仔鱼放入育苗池塘;H、育苗池塘的水质管理;I、增氧机的管理;J、仔鱼4~5日龄的饵料投喂及管理;K、仔鱼6~13日龄的饵料投喂及管理;L、仔鱼14~23日龄的饵料投喂及管理;M、仔鱼24~28日龄的饵料投喂及管理;N、仔鱼29~40日龄的饵料投喂及管理;O、波纹唇鱼的起捕。本发明应用于水产养殖技术领域,通过使用本发明所提供的波纹唇鱼的人工育苗方法,能批量培育幼鱼。该人工育苗方法科学合理、高效。The invention discloses a method for artificially cultivating seedlings of the corrugated lip fish, which comprises the following steps: A. selecting a seedling pond; B. disinfecting the seedling pond; C. filling the seedling pond; D. cultivating plankton; E. setting an incubator ; F, hatching of fertilized eggs; G, larvae put into the nursery pond; H, water quality management in the nursery pond; I, management of aeration machine; J, bait feeding and management of 4-5 days old larvae; 6-13 days old bait feeding and management; L, larvae 14-23 days old bait feeding and management; M, larvae 24-28 days old bait feeding and management; N, larvae 29-40 days old Feeding and management of bait; O, the catch of corrugated lip fish. The present invention is applied to the technical field of aquaculture, and the juvenile fish can be cultivated in batches by using the artificial seedling raising method of the wrasse provided by the present invention. The artificial seedling raising method is scientific, reasonable and efficient.
Description
技术领域technical field
本发明涉及水产养殖技术领域,具体涉及一种波纹唇鱼的人工育苗方法。The invention relates to the technical field of aquaculture, in particular to a method for artificially raising seedlings of the wrasse.
背景技术Background technique
波纹唇鱼(CheilinusundulatusRüppell)俗称苏眉、拿破仑鱼,隶属鲈形目、隆头鱼科、唇鱼属。它是体型最大的珊瑚礁鱼类之一,成体体长可达200cm。主要分布于非洲东岸,红海及从印度洋至太平洋中心,我国的南海、东海南部海域都有分布。波纹唇鱼是亚洲人盘中的美味佳肴,过渡捕捞等原因导致波纹唇鱼濒临绝种。2004年,世界自然基金会公布10种濒临灭绝的生物名单中,波纹唇鱼名列第二;同年该鱼种被列入IUCN(国际自然保护联盟)濒危动物红皮书;2005年被列入CITES《濒危野生动植物种国际贸易公约》附录Ⅱ。The corrugated lip fish (Cheilinus undulatus Rüppell), commonly known as Sumei and Napoleon fish, belongs to the order Perciformes, the family Wrasse, and the genus Cheilinus. It is one of the largest coral reef fishes, with adults up to 200cm in length. It is mainly distributed on the east coast of Africa, the Red Sea and from the Indian Ocean to the center of the Pacific Ocean. It is distributed in the South my country Sea and the southern part of the East China Sea. Corrugated lip fish is a delicacy on the plate of Asians. Due to overfishing and other reasons, the corrugated lip fish is on the verge of extinction. In 2004, the World Wildlife Fund announced the list of 10 endangered species, and the corrugated lip fish ranked second; in the same year, the fish species was included in the IUCN (International Union for Conservation of Nature) Red Book of Endangered Animals; in 2005, it was included in the CITES " Appendix II of the Convention on International Trade in Endangered Species of Wild Fauna and Flora.
波纹唇鱼被过度捕捞,资源衰退受到了高度重视,相关研究也逐渐增多。Sadovy等(2003)从波纹唇鱼的地理分布、栖息地、丰度、生物学、渔业资源与利用、贸易、海水养殖和保护状况等方面对过去的研究进行了较详细的综述报道,认为该鱼种尚未成功在孵化场饲养,需要紧急管理或保护。Sluka等(2005)调查印度西海岸的波纹唇鱼,发现其密度很低。Choat等(2006)研究了澳大利亚东北部波纹唇鱼的耳石发现,雄性的最大年龄为25龄,雌性的最大年龄为30龄,年死亡率为10%~14%,雌性先熟,但并不是所有的鱼都会性逆转。JohnE.Randall(1978)研究太平洋红海波纹唇鱼的食性,显示该鱼主要以软体动物,鱼类,海胆及甲壳类动物为食。Dorenbosch等(2006)调查了西印度洋四个岛屿的波纹唇鱼,认为海草床和红树林是其幼体的重要栖息地。P.L.Colin(2010)观察到波纹唇眉鱼每天高潮后聚集到堡礁向海边缘的一个特定位置产卵,认为该鱼产卵的周年变化与季节和月相有关。OlivierChateau和LaurentWantiez(2006)采用无线电遥测的方法对波纹唇鱼的家域大小和活动节律进行了研究。The corrugated lip fish has been overfished, and the resource decline has received great attention, and related research has gradually increased. Sadovy et al. (2003) conducted a detailed review report on the past research from the aspects of the geographical distribution, habitat, abundance, biology, fishery resources and utilization, trade, mariculture and protection status of the corrugated lip fish, and believed that the The fingerlings have not been successfully reared in hatcheries and require urgent management or conservation. Sluka et al. (2005) surveyed the chrysalis off the west coast of India and found its density to be very low. Choat et al. (2006) studied the otoliths of the corrugated lip fish in northeastern Australia and found that the maximum age of males is 25 years old, the maximum age of females is 30 years old, and the annual mortality rate is 10% to 14%. Females are precocious, but not Not all fish are sexually reversed. JohnE.Randall (1978) studied the feeding habits of the Pacific Red Sea corrugated lip fish, showing that the fish mainly feeds on molluscs, fish, sea urchins and crustaceans. Dorenbosch et al. (2006) surveyed the corrugated lip fish on four islands in the western Indian Ocean and considered seagrass beds and mangroves to be important habitats for their larvae. P.L.Colin (2010) observed that the corrugated lip browfish gathers to spawn at a specific location on the seaward edge of the barrier reef after high tide every day, and believes that the annual variation of the fish's spawning is related to seasons and moon phases. Olivier Chateau and Laurent Wantiez (2006) used the method of radio telemetry to study the home range size and activity rhythm of the corrugated lip fish.
在人工繁殖方面,Murdjani(1999)曾对波纹唇鱼进行人工诱导产卵,孵化率达30%~80%,但大部分仔鱼只能存活16天。国内对波纹唇鱼的研究较少,海南大学陈国华等(2009,2010)进行了波纹唇鱼染色体核型分析、消化系统的形态解剖和组织学研究,该鱼具染色体48条,为肉食性无胃鱼类,消化道全长极短,颌齿和咽齿都很发达,肠道极粗,肝脏分三叶,胰脏弥散性分布在肝脏组织中。在波纹唇鱼濒临绝种的情况下,以人工繁殖解决苗种问题,进而实现商业养殖,是保护波纹唇鱼的自然资源,满足人们对波纹唇鱼的需求的有效途径。In terms of artificial reproduction, Murdjani (1999) had artificially induced spawning of the chrysalis, with a hatching rate of 30% to 80%, but most of the larvae could only survive for 16 days. There are few domestic researches on the corrugated lip fish. Chen Guohua of Hainan University et al. (2009, 2010) conducted chromosome karyotype analysis, morphological anatomy and histological research on the digestive system of the corrugated lip fish. The fish has 48 chromosomes and is carnivorous without stomach. In fish, the overall length of the digestive tract is extremely short, the jaws and pharyngeal teeth are well developed, the intestines are extremely thick, the liver is divided into three lobes, and the pancreas is diffusely distributed in the liver tissue. Under the situation that the pleura is on the verge of extinction, it is an effective way to protect the natural resources of the pleura and meet people's demand for the pleura to solve the seed problem by artificial breeding and then realize commercial breeding.
发明内容Contents of the invention
本发明所要解决的问题是,针对上述技术的不足,设计出一种科学合理的、高效的、批量将波纹唇鱼受精卵培育成幼鱼的人工育苗方法。The problem to be solved by the present invention is to design a scientific, reasonable, efficient and artificial breeding method for cultivating the fertilized eggs of the chrysalis into juveniles in batches, aiming at the deficiencies of the above-mentioned technologies.
本发明为解决其问题所采用的技术方案是:The technical scheme that the present invention adopts for solving its problem is:
一种波纹唇鱼的人工育苗方法,包括以下步骤:A method for artificial breeding of corrugated lip fish, comprising the following steps:
A、育苗池塘的选取:选取灌水、排水便利、面积为2~5亩、池底平整、池深2.5m以上、蓄水深度2m以上、育苗期间水温为27~31.5℃、能取得清洁海水的池塘作为育苗池塘,育苗池塘的每亩水面积设置增氧机1台,在距育苗池塘边缘1m处设置一圈充气石,相连充气石的间距为1m,所述充气石连接于充气机;A. Selection of nursery ponds: Select irrigation ponds, convenient drainage, an area of 2 to 5 acres, a flat bottom, a pond depth of more than 2.5m, a water storage depth of more than 2m, a water temperature of 27 to 31.5°C during seedling cultivation, and access to clean seawater. The pond is used as a nursery pond, and the water area of each mu of the nursery pond is provided with 1 aeration machine, and a circle of aerated stones is set at 1m from the edge of the nursery pond, and the distance between the aerated stones is 1m, and the aerated stones are connected to the aerator;
B、育苗池塘的消毒:人工清除育苗池塘底部的杂物,主要包括污泥、浒苔,让育苗池塘在太阳下曝晒3~4d,灌水使育苗池塘水位深度达到10~20cm,对育苗池塘的每亩水面积用漂白粉10~20kg全池泼洒,用于消毒,确认育苗池塘中的鱼类、甲壳类、软体类全部死亡后,排出育苗池塘中的水,再让育苗池塘在太阳下晾晒1~2d;B. Disinfection of nursery ponds: Manually remove the sundries at the bottom of the nursery ponds, mainly including sludge and enteromorpha, let the nursery ponds be exposed to the sun for 3-4 days, and irrigate to make the water level of the nursery ponds reach 10-20cm deep. Sprinkle 10-20kg of bleaching powder on the whole pond per mu of water area for disinfection. After confirming that all the fish, crustaceans and molluscs in the nursery pond are dead, discharge the water in the nursery pond, and let the nursery pond dry in the sun for 1 ~2d;
C、育苗池塘的灌水:在购进受精卵的前4~5d,向育苗池塘灌水,使育苗池塘水位深度达到1.5~1.8m,灌进育苗池塘的海水事先已采用200目筛绢网制成的过滤袋过滤,保留水中的微型浮游生物,滤除小型浮游生物;C. Irrigation of the nursery pond: 4-5 days before the fertilized eggs are purchased, the nursery pond is filled with water to make the water level of the nursery pond reach 1.5-1.8m. The seawater poured into the nursery pond has been made of 200-mesh silk screen in advance filter bag filter, retain the micro plankton in the water, and filter out the small plankton;
D、培养浮游生物:对育苗池塘的每亩水面积泼洒生物有机肥颗粒30kg,启动增氧机和育苗池塘的充气机,促使施肥均匀,培育水质4~5d后,检查育苗池塘水体中的浮游生物情况;D. Cultivate plankton: Sprinkle 30kg of bio-organic fertilizer particles per mu of water area in the nursery pond, start the aerator and the aerator of the nursery pond to promote uniform fertilization, and after 4 to 5 days of cultivation water quality, check the plankton in the water body of the nursery pond biological condition;
E、设置孵化箱:孵化箱用彩条布制成,呈方形,规格为4m×3m×1m或6m×3m×1m,孵化箱通过固定架设置在育苗池塘内,使孵化箱的上沿高出育苗池塘水位0.1~0.15cm,在孵化箱内,按每平方米面积均匀设置充气石1只,所述充气石连接于充气机,并在孵化箱上方用90%遮光率的遮阳网遮盖,避免阳光直照,在购进受精卵的当天,从海区直接提取干净海水,水温要求27~29℃,海水经200目筛绢网制成的过滤袋过滤后灌入孵化箱,直至孵化箱水位深度达到0.85~0.9m,开启孵化箱的充气机,对孵化箱水体作不间断的充气;E. Set up the incubator: the incubator is made of colored striped cloth, in a square shape, with a size of 4m×3m×1m or 6m×3m×1m. The incubator is set in the nursery pond through a fixed frame, so that the upper edge of the incubator is high The water level of the nursery pond is 0.1-0.15cm. In the hatching box, one aerated stone is evenly arranged per square meter. The aerated stone is connected to the aerator and is covered with a sunshade net with a shading rate of 90% above the incubator. Avoid direct sunlight. On the day when fertilized eggs are purchased, clean seawater is directly extracted from the sea area. The water temperature is required to be 27-29°C. The seawater is filtered through a filter bag made of 200-mesh silk mesh and poured into the incubator until the water level of the incubator is reached. When the depth reaches 0.85-0.9m, turn on the inflator of the incubator to continuously inflate the water body of the incubator;
F、受精卵的孵化:将购进的受精卵连同包装袋放进孵化箱0.5~1h,直至包装袋内的水温与孵化箱的水温一致,拆开包装袋,让受精卵进入孵化箱中孵化,受精卵的孵化密度为200×103~300×103粒/m3,在充气条件下,受精卵在水体中翻滚,从受精开始计时,受精卵经15~16h后孵化出仔鱼,仔鱼孵出后留在孵化箱中培育3d;F. Incubation of fertilized eggs: Put the purchased fertilized eggs together with the packaging bag into the incubator for 0.5 to 1 hour until the water temperature in the packaging bag is consistent with that of the incubator, then unpack the bag and let the fertilized eggs enter the incubator to hatch , the hatching density of fertilized eggs is 200×10 3 to 300×10 3 grains/m 3 , under the condition of aeration, the fertilized eggs roll in the water body, counting from the time of fertilization, and the fertilized eggs hatch into larvae after 15 to 16 hours. After hatching, stay in the incubator and cultivate for 3 days;
G、仔鱼放入育苗池塘:仔鱼出膜后4日龄时,关闭育苗池塘的增氧机,开启育苗池塘的充气机作不间断充气,将孵化箱从固定架上解开,任孵化箱四个边沿部分沉入育苗池塘水平面之下,孵化箱内的充气石继续充气,任仔鱼在水流作用下缓慢游入育苗池塘,2~3h后,待孵化箱内的仔鱼全部进入育苗池塘,撤去孵化箱;G, the larvae are put into the nursery pond: when the larvae are 4 days old after they leave the film, turn off the aerator in the nursery pond, turn on the inflator in the nursery pond for uninterrupted inflation, untie the incubator from the fixed frame, and let the incubator four The edge part sinks below the level of the nursery pond, and the aerated stone in the hatch box continues to inflate, allowing the larvae to slowly swim into the nursery pond under the action of the water flow. After 2 to 3 hours, all the larvae in the hatch box enter the nursery pond, and remove the hatch. box;
H、育苗池塘的水质管理:以透明度为指标,保持育苗池塘透明度35~40cm,透明度过高时,从其他养鱼池塘或养虾池塘抽取富含单细胞藻类的海水加以调节,透明度过低时,在仔鱼15日龄前以加入干净海水加以调节,在仔鱼15日龄后通过换水加以调节;H. Water quality management in nursery ponds: take transparency as an indicator, and keep the transparency of the nursery pond at 35-40cm. When the transparency is too high, extract seawater rich in unicellular algae from other fish ponds or shrimp ponds for adjustment. When the transparency is too low , adjust by adding clean sea water before the larvae are 15 days old, and adjust by changing the water after the larvae are 15 days old;
I、增氧机的管理:在育苗池塘开始灌水培育水质时开启全部的增氧机,在仔鱼放入育苗池塘前关闭增氧机,在仔鱼10日龄时,开启一半数量的增氧机,在仔鱼15日龄时开启全部增氧机直至育苗结束;1, the management of aeration machine: start all aeration machines when raising water in the nursery pond, close the aeration machine before the larvae are put into the nursery pond, and when the larvae are 10 days old, open half of the aerators, When the larvae are 15 days old, all aerators are turned on until the nursery is finished;
J、仔鱼4~5日龄的饵料投喂及管理:以人工投喂的方式,主要利用育苗池塘培育的原生动物作为仔鱼饵料,保持育苗池塘水体中原生动物密度在0.1×106~1×106ind/l,控制轮虫密度不多于1×103ind/l,桡足类幼体密度不大于0.5×103ind/l,以免造成育苗池塘中单细胞藻类和原生动物的密度减少,当发现原生动物密度不足时,用贝类受精卵投入育苗池塘作为原生动物密度不足的补充,使饵料生物总密度达到要求;J. Feed feeding and management of larvae at 4 to 5 days old: artificially feeding, mainly using protozoa cultivated in nursery ponds as larvae bait, and keeping the density of protozoa in the water body of nursery ponds at 0.1×10 6 to 1× 10 6 ind/l, control the density of rotifers to no more than 1×10 3 ind/l, and the density of copepod larvae to no more than 0.5×10 3 ind/l, so as not to reduce the density of unicellular algae and protozoa in nursery ponds , when the density of protozoa is found to be insufficient, fertilized shellfish eggs are put into the nursery pond as a supplement to the insufficient density of protozoa, so that the total density of bait organisms meets the requirements;
K、仔鱼6~13日龄的饵料投喂及管理:以人工投喂的方式,向育苗池塘投入轮虫,保持轮虫密度3×103~4×103ind/l,保持原生动物密度0.1×106~1×106ind/l,原生动物密度不足时,用贝类受精卵投入育苗池塘作为补充,使饵料生物总密度达到要求;K. Feeding and management of larvae 6 to 13 days old: feeding rotifers into the nursery ponds artificially, keeping the density of rotifers at 3×10 3 to 4×10 3 ind/l, and maintaining the density of protozoa 0.1×10 6 ~1×10 6 ind/l, when the density of protozoa is insufficient, the fertilized eggs of shellfish are put into the nursery pond as a supplement, so that the total density of bait organisms can meet the requirements;
L、仔鱼14~23日龄的饵料投喂及管理:以人工投喂的方式,保持轮虫密度5×103~6×103ind/l,增加投入桡足类幼体,要求桡足类幼体密度3×103~5×103ind/l,任由原生动物密度减少;L. Feed feeding and management of larvae 14-23 days old: artificially feeding, keeping the density of rotifers at 5×10 3 ~6×10 3 ind/l, increasing the input of copepod larvae, requiring copepods The density of larvae is 3×10 3 ~5×10 3 ind/l, and the density of protozoa is allowed to decrease;
M、仔鱼24~28日龄的饵料投喂及管理:仔鱼在此期间开始长出鳞片,全长达到7~8mm,生活习性由浮游转为底栖,保持良好的水质和充足的饵料,保证顺利完成变态,以人工投喂的方式,保持轮虫密度5×103~6×103ind/l,增加投入桡足类幼体,要求桡足类幼体密度3×103~5×103ind/l,任由原生动物密度减少;M. Feed feeding and management of larvae aged 24-28 days: larvae begin to grow scales during this period, with a total length of 7-8mm, and their living habits change from planktonic to benthic. Maintain good water quality and sufficient bait to ensure Successfully complete metamorphosis, maintain the density of rotifers at 5×10 3 to 6×10 3 ind/l by artificial feeding, increase the input of copepod larvae, and require the density of copepod larvae to be 3×10 3 to 5×10 3 ind/l, let the protozoan density decrease;
N、仔鱼29~40日龄的饵料投喂及管理:以人工投喂的方式,保持育苗池塘桡足类幼体密度3×103~5×103ind/l,任由轮虫和原生动物密度减少;N. Feed feeding and management of 29-40-day-old larvae: artificial feeding is used to maintain the density of copepod larvae in the nursery pond at 3×10 3 to 5×10 3 ind/l, and rotifers and protozoa are allowed to Density reduction;
O、波纹唇鱼的起捕:在波纹唇鱼40日龄全长达到1.3~1.5cm时起捕,先排水使育苗池塘水位深度降至0.8m,选择鱼群多的一侧,使用布网围住育苗池塘的1/4面积,小心地将波纹唇鱼捕捞起,运到水泥池分规格、暂养,准备出售或转入下一阶段培育。O. The start of catching the corrugated lip fish: start catching when the full length of the corrugated lip fish reaches 1.3-1.5cm at the age of 40 days, first drain the water to reduce the water level of the nursery pond to 0.8m, choose the side with more fish, and use a cloth net Surround the 1/4 area of the nursery pond, carefully catch the corrugated lip fish, transport it to the cement pond for classification, and temporarily raise it for sale or transfer to the next stage of cultivation.
本发明的有益效果是:本发明应用于水产养殖技术领域,本发明提供的是选取育苗池塘,通过对灌进育苗池塘的海水进行过滤,有效控制育苗池塘水体中初始浮游生物的组成,将波纹唇鱼的受精卵在孵化箱中培育,防止仔鱼在没有主动游泳能力的情况下进入育苗池塘培育而沉底死亡,提高仔鱼的成活率,根据仔鱼不同发育阶段投喂合适的饵料生物,同时控制不宜的浮游生物数量,达到批量将波纹唇鱼受精卵培育成幼鱼的方法。该方法科学合理、高效。The beneficial effects of the present invention are: the present invention is applied to the technical field of aquaculture, and what the present invention provides is to select a nursery pond, filter the seawater poured into the nursery pond, effectively control the composition of the initial plankton in the water body of the nursery pond, and reduce the ripple The fertilized eggs of the lip fish are cultivated in the incubator to prevent the larvae from sinking to the bottom and die when they do not have the ability to swim actively, and to improve the survival rate of the larvae. According to the different developmental stages of the larvae, they are fed with suitable bait organisms, and at the same time control The unfavorable amount of plankton reaches the method of cultivating the fertilized eggs of the corrugated lip fish into juveniles in batches. The method is scientific, reasonable and efficient.
具体实施方式detailed description
以下将结合实施例对本发明的构思及产生的技术效果进行清楚、完整的描述,以充分地理解本发明的目的、特征和效果。显然,所描述的实施例只是本发明的一部分实施例,而不是全部实施例,基于本发明的实施例,本领域的技术人员在不付出创造性劳动的前提下所获得的其他实施例,均属于本发明保护的范围。The conception and technical effects of the present invention will be clearly and completely described below in conjunction with the embodiments, so as to fully understand the purpose, features and effects of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, rather than all of them. Based on the embodiments of the present invention, other embodiments obtained by those skilled in the art without creative efforts belong to The protection scope of the present invention.
本发明所提供的一种波纹唇鱼的人工育苗方法,包括以下步骤:A kind of artificial breeding method of corrugated lip fish provided by the present invention comprises the following steps:
A、育苗池塘的选取:选取灌水、排水便利、面积为2~5亩、池底平整、池深2.5m以上、蓄水深度2m以上、育苗期间水温为27~31.5℃、能取得清洁海水的池塘作为育苗池塘,育苗池塘的每亩水面积设置增氧机1台,在距育苗池塘边缘1m处设置一圈充气石,相连充气石的间距为1m,所述充气石连接于充气机。作为优选的实施方式,所选取的育苗池塘面积为4.5亩,所述增氧机为叶轮式增氧机,育苗池塘上盖有阻光率80%的遮阳网。A. Selection of nursery ponds: Select irrigation ponds, convenient drainage, an area of 2 to 5 acres, a flat bottom, a pond depth of more than 2.5m, a water storage depth of more than 2m, a water temperature of 27 to 31.5°C during seedling cultivation, and access to clean seawater. The pond is used as a nursery pond, and one aerator is set per mu of water area in the nursery pond, and a circle of aerated stones is set at 1m from the edge of the nursery pond, and the distance between the connected aerated stones is 1m, and the aerated stones are connected to the aerator. As a preferred embodiment, the selected nursery pond area is 4.5 mu, and the aerator is an impeller type aerator, and the seedling pond is covered with a sunshade net with a light blocking rate of 80%.
B、育苗池塘的消毒:人工清除育苗池塘底部的污泥、浒苔等杂物,让育苗池塘在太阳下曝晒3~4d,灌水使育苗池塘水位深度达到10~20cm,对育苗池塘的每亩水面积用漂白粉10~20kg全池泼洒,用于消毒,确认育苗池塘中的鱼类、甲壳类、软体类全部死亡后,排出育苗池塘中的水,再让育苗池塘在太阳下晾晒1~2d。作为优选的实施方式,清除育苗池塘底部的杂物后让育苗池塘在太阳下晾晒4d,进水完后对育苗池塘的每亩水面积用20kg漂白粉泼洒,排出育苗池塘中的水后,再让育苗池塘在太阳下晾晒2d。B. Disinfection of nursery ponds: Manually remove sludge, enteromorpha and other sundries at the bottom of the nursery ponds, expose the nursery ponds to the sun for 3 to 4 days, and irrigate to make the water level of the nursery ponds reach 10 to 20 cm. Sprinkle 10-20kg of bleaching powder on the water area of the pond for disinfection. After confirming that all fish, crustaceans and molluscs in the nursery pond are dead, drain the water in the nursery pond, and let the nursery pond dry in the sun for 1-2 days . As a preferred embodiment, remove the sundries at the bottom of the seedling pond and let the seedling pond air for 4d under the sun, after the water intake, the water area per mu of the seedling pond is sprinkled with 20kg of bleaching powder, after discharging the water in the seedling pond, let The nursery ponds were dried in the sun for 2 days.
C、育苗池塘的灌水:在购进受精卵的前4~5d,向育苗池塘灌水,使育苗池塘水位深度达到1.5~1.8m,作为优选的实施方式,育苗池塘水位深度应达到1.8m。灌进育苗池塘的海水事先已采用200目筛绢网制成的过滤袋过滤,保留水中的微型浮游生物,滤除小型浮游生物和更大的浮游生物,其中育苗池塘中的微型浮游生物主要包括单细胞藻类、原生动物等,小型浮游生物主要包括轮虫、桡足类幼体等。灌进育苗池塘的海水如果不经过200目过滤袋过滤,则育苗池塘中轮虫、桡足类幼体较多时会让单细胞藻类、原生动物数量增长慢,导致波纹唇鱼缺乏开口饵料。C, irrigation of nursery ponds: before purchasing fertilized eggs, pour water into the nursery ponds to make the water level depth of the nursery ponds reach 1.5-1.8m. As a preferred embodiment, the water level depths of the nursery ponds should reach 1.8m. The seawater poured into the nursery pond has been filtered with a filter bag made of 200-mesh silk net in advance to retain the micro plankton in the water and filter out small plankton and larger plankton. The micro plankton in the nursery pond mainly includes Single-celled algae, protozoa, etc., small plankton mainly include rotifers, copepod larvae, etc. If the seawater poured into the nursery pond is not filtered through a 200-mesh filter bag, when there are many rotifers and copepod larvae in the nursery pond, the number of single-celled algae and protozoa will grow slowly, resulting in the lack of open bait for the corrugated lip fish.
D、培养浮游生物:对育苗池塘的每亩水面积泼洒生物有机肥颗粒30kg,启动增氧机和育苗池塘的充气机,促使施肥均匀,培育水质4~5d后,检查育苗池塘水体中的浮游生物情况。作为优选的实施例,生物有机肥颗粒要求符合NY884-2004部颁标准(①有效活菌数≥0.2亿/g;②有机质含量≥25%;③水分≤15.0%;④pH值5.5-8.5;⑤粪大肠菌群数≤100个/g;⑥蛔虫卵死亡率≥95%);而水体中的浮游生物情况达到透明度37cm,硅藻1.47×106,绿藻1.265×106,金藻0.275×106,甲藻、蓝藻、隐藻合计0.825×106,原生动物0.816×106,贝类幼虫50×103,轮虫0.5×103,桡足类幼体0.5×103为较佳。D. Cultivate plankton: Sprinkle 30kg of bio-organic fertilizer particles per mu of water area in the nursery pond, start the aerator and the aerator of the nursery pond to promote uniform fertilization, and after 4 to 5 days of cultivation water quality, check the plankton in the water body of the nursery pond Biological situation. As a preferred embodiment, bio-organic fertilizer granules are required to meet the NY884-2004 ministerial standard (①The number of effective viable bacteria is ≥0.2 billion/g; ②The organic matter content is ≥25%; ③Moisture content≤15.0%; ④The pH value is 5.5-8.5; ⑤ The number of fecal coliforms ≤ 100/g; ⑥ The death rate of roundworm eggs ≥ 95%); while the plankton in the water body reaches a transparency of 37cm, diatoms 1.47×10 6 , green algae 1.265×10 6 , and golden algae 0.275× 10 6 , the total of dinoflagellates, cyanobacteria, and cryptophytes is 0.825×10 6 , protozoa 0.816×10 6 , shellfish larvae 50×10 3 , rotifers 0.5×10 3 , copepod larvae 0.5×10 3 are better.
E、设置孵化箱:孵化箱用彩条布制成,呈方形,规格为4m×3m×1m或6m×3m×1m,孵化箱通过固定架设置在育苗池塘内,使孵化箱的上沿高出育苗池塘水位0.1~0.15cm,在孵化箱内,按每平方米面积均匀设置充气石1只,所述充气石连接于充气机,并在孵化箱上方用90%遮光率的遮阳网遮盖,避免阳光直照,在购进受精卵的当天,从海区直接提取干净海水,水温要求27~29℃,海水经200目筛绢网制成的过滤袋过滤后灌入孵化箱,直至孵化箱水位深度达到0.85~0.9m,开启孵化箱的充气机,对孵化箱水体作不间断的充气。E. Set up the incubator: the incubator is made of colored striped cloth, in a square shape, with a size of 4m×3m×1m or 6m×3m×1m. The incubator is set in the nursery pond through a fixed frame, so that the upper edge of the incubator is high The water level of the nursery pond is 0.1-0.15cm. In the hatching box, one aerated stone is evenly arranged per square meter. The aerated stone is connected to the aerator and is covered with a sunshade net with a shading rate of 90% above the incubator. Avoid direct sunlight. On the day when fertilized eggs are purchased, clean seawater is directly extracted from the sea area. The water temperature is required to be 27-29°C. The seawater is filtered through a filter bag made of 200-mesh silk mesh and poured into the incubator until the water level of the incubator is reached. When the depth reaches 0.85-0.9m, turn on the inflator of the incubator to continuously inflate the water body of the incubator.
F、受精卵的孵化:将购进的受精卵连同包装袋放进孵化箱0.5~1h,直至包装袋内的水温与孵化箱的水温一致,拆开包装袋,让受精卵进入孵化箱中孵化,受精卵的孵化密度为200×103~300×103粒/m3,在充气条件下,受精卵在水体中翻滚,从受精开始计时,受精卵经15~16h后孵化出仔鱼,仔鱼孵出后留在孵化箱中培育3d。作为优选的实施方式,受精卵在孵化箱内的孵化密度为200×103粒/m3,孵化箱内水温29℃,胚胎发育历时16h后孵化出仔鱼,仔鱼在孵化箱中培育3d。刚孵化的仔鱼器官发育不完全,活动能力差,不能主动游泳,在水体中仅偶尔活动,仔鱼在孵化箱的第4天(仔鱼3日龄),全长3.0mm,活动能力增强,能主动游泳,并出现摄食动作。F. Incubation of fertilized eggs: Put the purchased fertilized eggs together with the packaging bag into the incubator for 0.5 to 1 hour until the water temperature in the packaging bag is consistent with that of the incubator, then unpack the bag and let the fertilized eggs enter the incubator to hatch , the hatching density of fertilized eggs is 200×10 3 to 300×10 3 grains/m 3 , under the condition of aeration, the fertilized eggs roll in the water body, counting from the time of fertilization, and the fertilized eggs hatch into larvae after 15 to 16 hours. After hatching, they were kept in the incubator for 3 days. As a preferred embodiment, the hatching density of fertilized eggs in the incubator is 200×10 3 grains/m 3 , the water temperature in the incubator is 29°C, the larvae hatch after 16 hours of embryo development, and the larvae are cultivated in the incubator for 3 days. The organs of the newly hatched larvae are not fully developed, their mobility is poor, they cannot actively swim, and they only move occasionally in the water body. On the 4th day in the incubator (larvae are 3 days old), the total length of the larvae is 3.0mm, their mobility is enhanced, and they can actively swim. Swimming, and feeding movements appear.
G、仔鱼放入育苗池塘:仔鱼出膜后4日龄时,关闭育苗池塘的增氧机,开启育苗池塘的充气机作不间断充气,将孵化箱从固定架上解开,任孵化箱四个边沿部分沉入育苗池塘水平面之下,孵化箱内的充气石继续充气,任仔鱼在水流作用下缓慢游入育苗池塘,2~3h后,待孵化箱内的仔鱼全部进入育苗池塘,撤去孵化箱。作为优选的实施例,在将仔鱼放入育苗池塘前,要求育苗池塘的水质指标达到透明度35cm,硅藻1.29×106/l,绿藻1.22×106ind/l,金藻0.41×106ind,甲藻、蓝藻、隐藻、黄藻合计0.66×106ind/l,原生动物0.815×106ind/l,贝类幼虫62.5×103ind/l,轮虫0.687×103ind/l,桡足类幼体0.417×103ind/l为较佳。G, the larvae are put into the nursery pond: when the larvae are 4 days old after they leave the film, turn off the aerator in the nursery pond, turn on the inflator in the nursery pond for uninterrupted inflation, untie the incubator from the fixed frame, and let the incubator four The edge part sinks below the level of the nursery pond, and the aerated stone in the hatch box continues to inflate, allowing the larvae to slowly swim into the nursery pond under the action of the water flow. After 2 to 3 hours, all the larvae in the hatch box enter the nursery pond, and remove the hatch. box. As a preferred embodiment, before the larvae are placed in the nursery pond, the water quality indicators of the nursery pond are required to reach a transparency of 35 cm, diatoms 1.29×10 6 /l, green algae 1.22×10 6 ind/l, and golden algae 0.41×10 6 ind, dinoflagellates, cyanobacteria, cryptophytes, and yellow algae total 0.66×10 6 ind/l, protozoa 0.815×10 6 ind/l, shellfish larvae 62.5×10 3 ind/l, rotifers 0.687×10 3 ind/l l, 0.417×10 3 ind/l for copepod larvae is better.
H、育苗池塘的水质管理:以透明度为指标,保持育苗池塘透明度35~40cm,透明度过高时,从其他养鱼池塘或养虾池塘抽取富含单细胞藻类的海水加以调节,透明度过低时,在仔鱼15日龄前以加入干净海水加以调节,在仔鱼15日龄后通过换水加以调节。H. Water quality management in nursery ponds: take transparency as an indicator, and keep the transparency of the nursery pond at 35-40cm. When the transparency is too high, extract seawater rich in unicellular algae from other fish ponds or shrimp ponds for adjustment. When the transparency is too low , before the larvae are 15 days old, they can be regulated by adding clean seawater, and after the larvae are 15 days old, they can be regulated by changing the water.
I、增氧机的管理:在育苗池塘开始灌水培育水质时开启全部的增氧机,在仔鱼放入育苗池塘前关闭增氧机,在仔鱼10日龄时,开启一半数量的增氧机,在仔鱼15日龄时开启全部增氧机直至育苗结束。1, the management of aeration machine: start all aeration machines when raising water in the nursery pond, close the aeration machine before the larvae are put into the nursery pond, and when the larvae are 10 days old, open half of the aerators, When the larvae were 15 days old, all aerators were turned on until the nursery was finished.
J、仔鱼4~5日龄的饵料投喂及管理:以人工投喂的方式,主要利用育苗池塘培育的原生动物作为仔鱼饵料,保持育苗池塘水体中原生动物密度在0.1×106~1×106ind/l,控制轮虫密度不多于1×103ind/l,桡足类幼体密度不大于0.5×103ind/l,以免造成育苗池塘中单细胞藻类和原生动物的密度减少,当发现原生动物密度不足时,用贝类受精卵投入育苗池塘作为原生动物密度不足的补充,使饵料生物总密度达到要求。作为优选的实施方式,贝类受精卵选取牡蛎受精卵为佳。J. Feed feeding and management of larvae at 4 to 5 days old: artificially feeding, mainly using protozoa cultivated in nursery ponds as larvae bait, and keeping the density of protozoa in the water body of nursery ponds at 0.1×10 6 to 1× 10 6 ind/l, control the density of rotifers to no more than 1×10 3 ind/l, and the density of copepod larvae to no more than 0.5×10 3 ind/l, so as not to reduce the density of unicellular algae and protozoa in nursery ponds , when the density of protozoa is found to be insufficient, shellfish fertilized eggs are put into the nursery pond as a supplement to the insufficient density of protozoa, so that the total density of bait organisms can meet the requirements. As a preferred embodiment, the shellfish fertilized eggs are preferably oyster fertilized eggs.
K、仔鱼6~13日龄的饵料投喂及管理:以人工投喂的方式,向育苗池塘投入轮虫,保持轮虫密度3×103~4×103ind/l,保持原生动物密度0.1×106~1×106ind/l,原生动物密度不足时,用贝类受精卵投入育苗池塘作为补充,使饵料生物总密度达到要求。K. Feeding and management of larvae 6 to 13 days old: feeding rotifers into the nursery ponds artificially, keeping the density of rotifers at 3×10 3 to 4×10 3 ind/l, and maintaining the density of protozoa 0.1×10 6 ~1×10 6 ind/l, when the density of protozoa is insufficient, the fertilized eggs of shellfish are put into the nursery pond as a supplement, so that the total density of bait organisms can meet the requirements.
L、仔鱼14~23日龄的饵料投喂及管理:以人工投喂的方式,保持轮虫密度5×103~6×103ind/l,增加投入桡足类幼体,要求桡足类幼体密度3×103~5×103ind/l,任由原生动物密度减少。L. Feed feeding and management of larvae 14-23 days old: artificially feeding, keeping the density of rotifers at 5×10 3 ~6×10 3 ind/l, increasing the input of copepod larvae, requiring copepods The density of larvae is 3×10 3 ~5×10 3 ind/l, and the density of protozoa is allowed to decrease.
M、仔鱼24~28日龄的饵料投喂及管理:仔鱼在此期间开始长出鳞片,全长达到7~8mm,生活习性由浮游转为底栖,保持良好的水质和充足的饵料,保证顺利完成变态,以人工投喂的方式,保持轮虫密度5×103~6×103ind/l,增加投入桡足类幼体,要求桡足类幼体密度3×103~5×103ind/l,任由原生动物密度减少。M. Feed feeding and management of larvae aged 24-28 days: larvae begin to grow scales during this period, with a total length of 7-8mm, and their living habits change from planktonic to benthic. Maintain good water quality and sufficient bait to ensure Successfully complete metamorphosis, maintain the density of rotifers at 5×10 3 to 6×10 3 ind/l by artificial feeding, increase the input of copepod larvae, and require the density of copepod larvae to be 3×10 3 to 5×10 3 ind/l, let the protozoan density decrease.
N、仔鱼29~40日龄的饵料投喂及管理:以人工投喂的方式,保持育苗池塘桡足类幼体密度3×103~5×103ind/l,任由轮虫和原生动物密度减少。N. Feed feeding and management of 29-40-day-old larvae: artificial feeding is used to maintain the density of copepod larvae in the nursery pond at 3×10 3 to 5×10 3 ind/l, and rotifers and protozoa are allowed to Density decreases.
O、波纹唇鱼的起捕:在波纹唇鱼40日龄全长达到1.3~1.5cm时起捕,先排水使育苗池塘水位深度降至0.8m,选择鱼群多的一侧,使用布网围住育苗池塘的1/4面积,小心地将波纹唇鱼捕捞起,运到水泥池分规格、暂养,准备出售或转入下一阶段培育。由于育苗池塘水源直接取自大海,育苗池塘水体虽经过200目过滤袋过滤,但无法全部滤除蟹类的无节幼体,后者在育苗池塘中伴随仔鱼生长发育,当波纹唇鱼变态为底栖生活,很容易被蟹类捕捉,故应尽早起捕,又由于波纹唇鱼已经长齐鳞被,可以经受起捕的刺激。O. The start of catching the corrugated lip fish: start catching when the full length of the corrugated lip fish reaches 1.3-1.5cm at the age of 40 days, first drain the water to reduce the water level of the nursery pond to 0.8m, choose the side with more fish, and use a cloth net Surround the 1/4 area of the nursery pond, carefully catch the corrugated lip fish, transport it to the cement pond for classification, and temporarily raise it for sale or transfer to the next stage of cultivation. Since the water source of the nursery pond is directly taken from the sea, although the water body of the nursery pond is filtered through a 200-mesh filter bag, it cannot completely filter out the nauplius of crabs. The latter grows and develops with the larvae in the nursery pond. It is easy to be caught by crabs, so it should be caught as soon as possible, and because the corrugated lip fish has already grown scales, it can withstand the stimulation of catching.
运输受精卵需要计算好启运时间,确保运输途中仔鱼不会孵出。如果运输时间较长,以受精卵运输的方式不能在仔鱼出膜前到达目的地时,则应改为运输仔鱼。待仔鱼全部出膜后,即可收苗、包装、运输。When transporting fertilized eggs, it is necessary to calculate the departure time to ensure that the larvae will not hatch during transportation. If the transportation time is long and the method of fertilized egg transportation cannot reach the destination before the larvae come out of the membrane, the larvae should be transported instead. After all the larvae come out of the film, the seedlings can be harvested, packaged and transported.
以上对本发明的较佳实施方式进行了具体说明,但本发明并不限于所述实施例,熟悉本领域的技术人员在不违背本发明精神的前提下还可作出种种的等同变型或替换,这些等同的变型或替换均包含在本申请权利要求所限定的范围内。The preferred embodiments of the present invention have been described in detail above, but the present invention is not limited to the described embodiments, and those skilled in the art can also make various equivalent modifications or replacements without departing from the spirit of the present invention. Equivalent modifications or replacements are all included within the scope defined by the claims of the present application.
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