CN102763505B - A kind of method of breaking Taxus chinensis var. mairei Seeds dormancy - Google Patents
A kind of method of breaking Taxus chinensis var. mairei Seeds dormancy Download PDFInfo
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- CN102763505B CN102763505B CN201110113040.3A CN201110113040A CN102763505B CN 102763505 B CN102763505 B CN 102763505B CN 201110113040 A CN201110113040 A CN 201110113040A CN 102763505 B CN102763505 B CN 102763505B
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- taxus chinensis
- chinensis var
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- 241000508252 Taxus mairei Species 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 25
- 230000005059 dormancy Effects 0.000 title claims abstract description 12
- 241001116500 Taxus Species 0.000 claims abstract description 13
- 238000009331 sowing Methods 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- 239000002245 particle Substances 0.000 claims description 15
- 230000035800 maturation Effects 0.000 claims description 7
- 239000004576 sand Substances 0.000 claims description 7
- 241000736199 Paeonia Species 0.000 claims description 5
- 235000006484 Paeonia officinalis Nutrition 0.000 claims description 5
- 238000005553 drilling Methods 0.000 claims description 5
- 210000003608 fece Anatomy 0.000 claims description 5
- 239000003864 humus Substances 0.000 claims description 5
- 239000010871 livestock manure Substances 0.000 claims description 5
- 238000010899 nucleation Methods 0.000 claims description 5
- 239000002689 soil Substances 0.000 claims description 5
- 229930191978 Gibberellin Natural products 0.000 claims description 4
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims description 4
- 239000003448 gibberellin Substances 0.000 claims description 4
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 3
- 238000009395 breeding Methods 0.000 abstract description 3
- 230000001488 breeding effect Effects 0.000 abstract description 3
- 238000007654 immersion Methods 0.000 abstract description 3
- 241001149649 Taxus wallichiana var. chinensis Species 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 229930012538 Paclitaxel Natural products 0.000 description 4
- 229960001592 paclitaxel Drugs 0.000 description 4
- RCINICONZNJXQF-MZXODVADSA-N taxol Chemical compound O([C@@H]1[C@@]2(C[C@@H](C(C)=C(C2(C)C)[C@H](C([C@]2(C)[C@@H](O)C[C@H]3OC[C@]3([C@H]21)OC(C)=O)=O)OC(=O)C)OC(=O)[C@H](O)[C@@H](NC(=O)C=1C=CC=CC=1)C=1C=CC=CC=1)O)C(=O)C1=CC=CC=C1 RCINICONZNJXQF-MZXODVADSA-N 0.000 description 4
- 238000004113 cell culture Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 238000011275 oncology therapy Methods 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
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- Pretreatment Of Seeds And Plants (AREA)
Abstract
The present invention relates to a kind of method of breaking Taxus chinensis var. mairei Seeds dormancy, it is characterized in that utilizing alternating temperature to process the seed of southerm yew, comprise the steps: a, immersion; B, storage; C, sowing. The object of the invention is to southerm yew and introduce a fine variety the problem that the cultivation cycle is long, cost is high, technical difficulty is large, provide a kind of simple to operate, what cost was low breaks Taxus chinensis var. mairei Seeds dormancy method. A kind of method of breaking Taxus chinensis var. mairei Seeds dormancy of the present invention, sows emergence rate then and can reach 30% by adopting this method to realize. Second Year 50% left and right of can also emerging again, has improved breeding efficiency.
Description
Technical field
The present invention relates to a kind of method of breaking Taxus chinensis var. mairei Seeds dormancy.
Background technology
Southerm yew is China first class of protection wild plant, is also very important a kind of woody medicinal species, contains in recent yearsOne of the most effective cancer therapy drug taxol. According to statistics, global taxol annual requirement 3000kg. In the market demand huge andWhen seriously supply falls short of demand, countries in the world are in order to protect these ancient endangered plants of Chinese yew, and numerous and confused legislation forbids it to enterRow commerciality gathers and utilizes, and " CITS " also listed in annex II, requires strictIts international trade of specification, prevents consuming excessively of resource. The disparities between supply and demand of having aggravated thus taxol market, resource has become restrictionThe bottleneck of plant cell culture enterprise development. Both at home and abroad the tame research of Chinese yew (comprising southerm yew) is entered to some extentExhibition, especially to taxus chinensis in northeast carried out bioecology characteristic, synecology, Forest Cultivation technology, wood structure andThe systematic research of the aspect such as physical property and chemical composition; Also have the chemical composition of forefathers to southerm yew, pharmacological action,Dormancy Mechanism, cottage propagation etc. are studied, but system not. Because Chinese yew (comprising southerm yew) is introduced a fine variety trainingThe cycle of planting is long, cost is high, technical difficulty is large, and the taxus resource amount that present stage cultivates can not meet plant cell culture far awayThe requirement of enterprise. According in November, 2005, Ministry of Forestry verifies, and national Chinese yew raw material forest storage is little, Yunnan, main producing region raw material forestAlso only have 642.06 ten thousand strains, the rare problem in medicine source of taxol still cannot solve in a short time. What this method proposed breaks notThe method of sleeping is different from existing bibliographical information. This method has realized to be sowed emergence rate then and can reach 30%. Second Year can also go out againSeedling 50% left and right, has improved breeding efficiency.
Summary of the invention
The object of the invention is to southerm yew and introduce a fine variety the problem that the cultivation cycle is long, cost is high, technical difficulty is large, provide oneKind is simple to operate, and what cost was low breaks Taxus chinensis var. mairei Seeds dormancy method.
A kind of method of breaking Taxus chinensis var. mairei Seeds dormancy of the present invention, is characterized in that utilizing alternating temperature to process the kind of southerm yewSon, comprises the steps:
A, immersion: after the abundant maturation of seed, gather, be then soaked in water pulp is rotted, with manually rubbing with the hands and remove pulp;
B, storage: soak and go damp sand in pulp seed chamber to preserve to Second Year at the beginning of 4 months;
C, sowing: by sand store seed 35 DEG C of-40 DEG C of gibberellin aqueous solution with 400ppm～600ppm or 1000ppm～The IBA of 1500ppm or 400ppm～600ppmNAA or 100ppmIAA soak 2 hours, then sowing.
A kind of method of breaking Taxus chinensis var. mairei Seeds dormancy of the present invention, comprises the steps:
A, immersion: after the abundant maturation of seed, gather, be then soaked in water pulp is rotted, the seed time of being soaked in water is 30My god-50 days. Remove pulp with artificial stranding;
B, storage: soak and go damp sand in pulp seed chamber to preserve to Second Year at the beginning of 4 months; Indoor holding conditions is 8 DEG C-15 DEG C and wetsSand humidity is 30%.
C, sowing: by sand store seed 35 DEG C of-40 DEG C of gibberellin aqueous solution with 400ppm～600ppm or 1000ppm～The IBA of 1500ppm or 400ppm～600ppmNAA or 100ppmIAA soak 2 hours, control water when sand is store to seed immersionTemperature is constant in 40 DEG C. Then sowing.
A kind of method of breaking Taxus chinensis var. mairei Seeds dormancy of the present invention, sows emergence rate then by adopting this method to realizeCan reach 30%. Second Year 50% left and right of can also emerging again, has improved breeding efficiency. In October, 2010 is by Xiangfan scientific and technological informationIt is high that office's current Taxus chinensis var. mairei Seeds germination percentage of update search and time are all not so good as this method.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention is further illustrated:
The southerm yew of example 1:1, selection robust growth is set as stock tree.
2, Taxus chinensis var. mairei Seeds pericarp becomes peony mid or late October, fully after maturation, gathers, and deposits in indoor shady and cool place and protectsDeposit, temperature is 20 DEG C of left and right.
3, late October is rotted the Taxus chinensis var. mairei Seeds of the gathering 40 angel's pulp that are soaked in water, and removes pulp with artificial stranding, then usesClear water washes away pulp, pericarp, dries. Moistening silt particle and Taxus chinensis var. mairei Seeds are mixed in 10: 1 ratios, select shady and coolIndoor storage, temperature, at 8-15 DEG C, is preserved to Second Year at the beginning of 4 months, keeps at any time silt particle moistening, damp sand humidity approximatelyBe 30%.
4, Second Year sifts out silt particle April with sieve, cleans Taxus chinensis var. mairei Seeds with clear water, then uses the gibberellin of 40 DEG C of 500ppmAqueous solution soaking 2 hours, selects fine day to sow.
5, select the sandy loam that the gradient is mild, soil layer is deep, draining is good, be rich in humus to do Seedlings nursery, in conjunction with whole every muApply the barnyard manure 3000～4000kg becoming thoroughly decomposed. At the beginning of 4 months, open railway carriage or compartment, the wide 1.2m in railway carriage or compartment, furrow width 30cm, the high 10～20cm in railway carriage or compartment.Seeding method, taking drilling as main, also can broadcast sowing.
6, sow that June, emergence rate was 31.5% then. Second Year emergence rate in June reaches 83%.
The southerm yew of example 2:1, selection robust growth is set as stock tree.
2, Taxus chinensis var. mairei Seeds pericarp becomes peony mid or late October, fully after maturation, gathers, and deposits in indoor shady and cool place and protectsDeposit, temperature is 20 DEG C of left and right.
3, late October is rotted the Taxus chinensis var. mairei Seeds of the gathering 40 angel's pulp that are soaked in water, and removes pulp with artificial stranding, then usesClear water washes away pulp, pericarp, dries. Moistening silt particle and Taxus chinensis var. mairei Seeds are mixed in 10: 1 ratios, select shady and coolIndoor storage, temperature, at 8-15 DEG C, is preserved to Second Year at the beginning of 4 months, keeps at any time silt particle moistening.
4, Second Year sifts out silt particle April with sieve, cleans Taxus chinensis var. mairei Seeds with clear water, then uses the IBA of 40 DEG C of 1500ppmAqueous solution soaking 2 hours, selects fine day to sow.
5, select the sandy loam that the gradient is mild, soil layer is deep, draining is good, be rich in humus to do Seedlings nursery, in conjunction with whole every muApply the barnyard manure 3000～4000kg becoming thoroughly decomposed. At the beginning of 4 months, open railway carriage or compartment, the wide 1.2m in railway carriage or compartment, furrow width 30cm, the high 10～20cm in railway carriage or compartment.Seeding method, taking drilling as main, also can broadcast sowing.
6, sow that June, emergence rate was 27% then. Second Year emergence rate in June reaches 78%.
The southerm yew of example 3:1, selection robust growth is set as stock tree.
2, Taxus chinensis var. mairei Seeds pericarp becomes peony mid or late October, fully after maturation, gathers, and deposits in indoor shady and cool place and protectsDeposit, temperature is 20 DEG C of left and right.
3, late October is rotted the Taxus chinensis var. mairei Seeds of the gathering 40 angel's pulp that are soaked in water, and removes pulp with artificial stranding, then usesClear water washes away pulp, pericarp, dries. Moistening silt particle and Taxus chinensis var. mairei Seeds are mixed in 10: 1 ratios, select shady and coolIndoor storage, temperature, at 8-15 DEG C, is preserved to Second Year at the beginning of 4 months, keeps at any time silt particle moistening.
4, Second Year sifts out silt particle April with sieve, cleans Taxus chinensis var. mairei Seeds with clear water, then uses the NAA water of 40 DEG C of 500ppmSolution soaks 2 hours, selects fine day to sow.
5, select the sandy loam that the gradient is mild, soil layer is deep, draining is good, be rich in humus to do Seedlings nursery, in conjunction with whole every muApply the barnyard manure 3000～4000kg becoming thoroughly decomposed. At the beginning of 4 months, open railway carriage or compartment, the wide 1.2m in railway carriage or compartment, furrow width 30cm, the high 10～20cm in railway carriage or compartment.Seeding method, taking drilling as main, also can broadcast sowing.
6, sow that June, emergence rate was 25% then. Second Year emergence rate in June reaches 80%.
The southerm yew of example 4:1, selection robust growth is set as stock tree.
2, Taxus chinensis var. mairei Seeds pericarp becomes peony mid or late October, fully after maturation, gathers, and deposits in indoor shady and cool place and protectsDeposit, temperature is 20 DEG C of left and right.
3, late October is rotted the Taxus chinensis var. mairei Seeds of the gathering 40 angel's pulp that are soaked in water, and removes pulp with artificial stranding, then usesClear water washes away pulp, pericarp, dries. Moistening silt particle and Taxus chinensis var. mairei Seeds are mixed in 10: 1 ratios, select shady and coolIndoor storage, temperature, at 8-15 DEG C, is preserved to Second Year at the beginning of 4 months, keeps at any time silt particle moistening.
4, Second Year sifts out silt particle April with sieve, cleans Taxus chinensis var. mairei Seeds with clear water, then uses the IAA water of 40 DEG C of 100ppmSolution soaks 2 hours, selects fine day to sow.
5, select the sandy loam that the gradient is mild, soil layer is deep, draining is good, be rich in humus to do Seedlings nursery, in conjunction with whole every muApply the barnyard manure 3000～4000kg becoming thoroughly decomposed. At the beginning of 4 months, open railway carriage or compartment, the wide 1.2m in railway carriage or compartment, furrow width 30cm, the high 10～20cm in railway carriage or compartment.Seeding method, taking drilling as main, also can broadcast sowing.
6, sow that June, emergence rate was 29% then. Second Year emergence rate in June reaches 81%.
Claims (1)
1. break a method for Taxus chinensis var. mairei Seeds dormancy, it is characterized in that utilizing alternating temperature to process southerm yewSeed, comprise the steps:
(1), select the southerm yew of robust growth to set as stock tree;
(2), mid or late October Taxus chinensis var. mairei Seeds pericarp become peony, fully after maturation, gather, deposit in chamberInterior shady and cool place preserves, and temperature is at 20 DEG C;
(3), late October by the Taxus chinensis var. mairei Seeds of gathering be soaked in water 40 angel's pulp rot, with manually rub with the handsRemove pulp, then wash away pulp, pericarp with clear water, dry; Moistening silt particle and Taxus chinensis var. mairei Seeds are pressed10:1 ratio mixes, and selects shady and cool indoor storage, and temperature is at 8-15 DEG C, preserves to Second Year AprilJust, keep at any time silt particle moistening, damp sand humidity is 30%;
(4), Second Year sifts out silt particle April with sieve, clean Taxus chinensis var. mairei Seeds with clear water, then use 40 DEG CThe gibberellin aqueous solution soaking of 500ppm 2 hours, selects fine day to sow;
(5), select the sandy loam that the gradient is mild, soil layer is deep, draining is good, be rich in humus to do Seedlings nursery,Apply in conjunction with whole every mu the barnyard manure 3000～4000kg becoming thoroughly decomposed; At the beginning of 4 months, open railway carriage or compartment, the wide 1.2m in railway carriage or compartment, ditchWide 30cm, the high 10～20cm in railway carriage or compartment; Seeding method, taking drilling as main, also can broadcast sowing;
(6), sow that June, emergence rate was 31.5% then; Second Year emergence rate in June reaches 83%.
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| CN201110113040.3A CN102763505B (en) | 2011-05-04 | 2011-05-04 | A kind of method of breaking Taxus chinensis var. mairei Seeds dormancy |
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| CN201110113040.3A CN102763505B (en) | 2011-05-04 | 2011-05-04 | A kind of method of breaking Taxus chinensis var. mairei Seeds dormancy |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102972191A (en) * | 2012-12-11 | 2013-03-20 | 雷学军 | Method for breeding and cultivating taxus mairei with seed |
| CN103477856A (en) * | 2013-09-26 | 2014-01-01 | 中南林业科技大学 | Taxus mairei planting method |
| CN105052940A (en) * | 2015-08-03 | 2015-11-18 | 佛山市顺德区宝铜金属科技有限公司 | Composition for breaking seed dormancy |
| CN107996248A (en) * | 2017-12-07 | 2018-05-08 | 浙江里光农业开发有限公司 | A kind of metasequoia method for cultivating seeds that can improve metasequoia survival rate |
| CN108718603A (en) * | 2018-06-22 | 2018-11-02 | 东北林业大学 | A method of promoting Germination of Taxus mairei Seeds |
| CN108934268A (en) * | 2018-07-03 | 2018-12-07 | 东北林业大学 | A method of abolishing Chinese yew seed dormancy |
| CN108934272B (en) * | 2018-07-31 | 2021-01-29 | 广西壮族自治区林业科学研究院 | Method for promoting germination of seeds of sequoia jiangnanensis |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1711842A (en) * | 2005-07-15 | 2005-12-28 | 中国科学院长春应用化学研究所 | Plant growth regulator composition for seed germination |
| CN1759653A (en) * | 2005-10-28 | 2006-04-19 | 林敏� | Method for planting southerm yew |
| CN1774987A (en) * | 2005-12-08 | 2006-05-24 | 褚菊芬 | Southern Chinese year potted landscpe cultivating method |
| WO2009109402A2 (en) * | 2008-03-07 | 2009-09-11 | S.W. Patentverwertungs Limited | Composition and uses for influencing hair growth |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1711842A (en) * | 2005-07-15 | 2005-12-28 | 中国科学院长春应用化学研究所 | Plant growth regulator composition for seed germination |
| CN1759653A (en) * | 2005-10-28 | 2006-04-19 | 林敏� | Method for planting southerm yew |
| CN1774987A (en) * | 2005-12-08 | 2006-05-24 | 褚菊芬 | Southern Chinese year potted landscpe cultivating method |
| WO2009109402A2 (en) * | 2008-03-07 | 2009-09-11 | S.W. Patentverwertungs Limited | Composition and uses for influencing hair growth |
Non-Patent Citations (2)
| Title |
|---|
| 不同处理对南方红豆杉种子萌发的影响;吉前华 等;《安徽农业科学》;20071110;第35卷(第31期);第9858-9859页 * |
| 温度和植物激素对提高南方红豆杉种子出苗率的研究;周洪英 等;《种子》;20070531;第26卷(第5期);第13页，第15页表3 * |
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