CN102657288B - Formula feed for chickens and preparation method thereof - Google Patents
Formula feed for chickens and preparation method thereof Download PDFInfo
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- CN102657288B CN102657288B CN201210063677.0A CN201210063677A CN102657288B CN 102657288 B CN102657288 B CN 102657288B CN 201210063677 A CN201210063677 A CN 201210063677A CN 102657288 B CN102657288 B CN 102657288B
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- vitamin
- salt
- enterococcus faecalis
- chick
- mixed feed
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- 238000002360 preparation method Methods 0.000 title claims description 15
- 241000287828 Gallus gallus Species 0.000 title abstract description 10
- 235000013330 chicken meat Nutrition 0.000 title abstract description 10
- 241000194032 Enterococcus faecalis Species 0.000 claims abstract description 37
- 229940032049 enterococcus faecalis Drugs 0.000 claims abstract description 34
- 150000001875 compounds Chemical class 0.000 claims abstract description 30
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 24
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 16
- 108090000790 Enzymes Proteins 0.000 claims abstract description 13
- 102000004190 Enzymes Human genes 0.000 claims abstract description 13
- 150000003839 salts Chemical class 0.000 claims abstract description 13
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims abstract description 12
- 229930003268 Vitamin C Natural products 0.000 claims abstract description 12
- 239000000843 powder Substances 0.000 claims abstract description 12
- 235000019154 vitamin C Nutrition 0.000 claims abstract description 12
- 239000011718 vitamin C Substances 0.000 claims abstract description 12
- 235000019764 Soybean Meal Nutrition 0.000 claims abstract description 9
- 239000004455 soybean meal Substances 0.000 claims abstract description 9
- 239000001763 2-hydroxyethyl(trimethyl)azanium Substances 0.000 claims abstract description 8
- 235000019743 Choline chloride Nutrition 0.000 claims abstract description 8
- 235000019733 Fish meal Nutrition 0.000 claims abstract description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 8
- 244000068988 Glycine max Species 0.000 claims abstract description 8
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 8
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims abstract description 8
- 239000005862 Whey Substances 0.000 claims abstract description 8
- 102000007544 Whey Proteins Human genes 0.000 claims abstract description 8
- 108010046377 Whey Proteins Proteins 0.000 claims abstract description 8
- FUFJGUQYACFECW-UHFFFAOYSA-L calcium hydrogenphosphate Chemical compound [Ca+2].OP([O-])([O-])=O FUFJGUQYACFECW-UHFFFAOYSA-L 0.000 claims abstract description 8
- SGMZJAMFUVOLNK-UHFFFAOYSA-M choline chloride Chemical compound [Cl-].C[N+](C)(C)CCO SGMZJAMFUVOLNK-UHFFFAOYSA-M 0.000 claims abstract description 8
- 229960003178 choline chloride Drugs 0.000 claims abstract description 8
- 235000019700 dicalcium phosphate Nutrition 0.000 claims abstract description 8
- 239000004467 fishmeal Substances 0.000 claims abstract description 8
- 239000008103 glucose Substances 0.000 claims abstract description 8
- 229930182817 methionine Natural products 0.000 claims abstract description 8
- 239000004575 stone Substances 0.000 claims abstract description 8
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 15
- 235000013312 flour Nutrition 0.000 claims description 14
- 239000002994 raw material Substances 0.000 claims description 14
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 10
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 10
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 claims description 10
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 claims description 10
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 10
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 10
- NHZMQXZHNVQTQA-UHFFFAOYSA-N pyridoxamine Chemical compound CC1=NC=C(CO)C(CN)=C1O NHZMQXZHNVQTQA-UHFFFAOYSA-N 0.000 claims description 10
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 claims description 8
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 8
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 8
- 239000011630 iodine Substances 0.000 claims description 8
- 229910052740 iodine Inorganic materials 0.000 claims description 8
- AZJQQNWSSLCLJN-UHFFFAOYSA-N 2-ethoxyquinoline Chemical compound C1=CC=CC2=NC(OCC)=CC=C21 AZJQQNWSSLCLJN-UHFFFAOYSA-N 0.000 claims description 7
- 241000482268 Zea mays subsp. mays Species 0.000 claims description 7
- 238000005469 granulation Methods 0.000 claims description 6
- 230000003179 granulation Effects 0.000 claims description 6
- 108091005508 Acid proteases Proteins 0.000 claims description 5
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 5
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 claims description 5
- MJVAVZPDRWSRRC-UHFFFAOYSA-N Menadione Chemical compound C1=CC=C2C(=O)C(C)=CC(=O)C2=C1 MJVAVZPDRWSRRC-UHFFFAOYSA-N 0.000 claims description 5
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 claims description 5
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 claims description 5
- 229930003451 Vitamin B1 Natural products 0.000 claims description 5
- 229930003471 Vitamin B2 Natural products 0.000 claims description 5
- 229930003427 Vitamin E Natural products 0.000 claims description 5
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 5
- 229960002685 biotin Drugs 0.000 claims description 5
- 235000020958 biotin Nutrition 0.000 claims description 5
- 239000011616 biotin Substances 0.000 claims description 5
- 239000011575 calcium Substances 0.000 claims description 5
- 229910052791 calcium Inorganic materials 0.000 claims description 5
- ASARMUCNOOHMLO-WLORSUFZSA-L cobalt(2+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2s)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+2].[N-]([C@@H]1[C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@H](C)OP([O-])(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O ASARMUCNOOHMLO-WLORSUFZSA-L 0.000 claims description 5
- 239000001530 fumaric acid Substances 0.000 claims description 5
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 5
- 239000004310 lactic acid Substances 0.000 claims description 5
- 235000014655 lactic acid Nutrition 0.000 claims description 5
- 229960003512 nicotinic acid Drugs 0.000 claims description 5
- 235000001968 nicotinic acid Nutrition 0.000 claims description 5
- 239000011664 nicotinic acid Substances 0.000 claims description 5
- 235000008151 pyridoxamine Nutrition 0.000 claims description 5
- 239000011699 pyridoxamine Substances 0.000 claims description 5
- 229960002477 riboflavin Drugs 0.000 claims description 5
- 239000011669 selenium Substances 0.000 claims description 5
- 229910052711 selenium Inorganic materials 0.000 claims description 5
- 229960003495 thiamine Drugs 0.000 claims description 5
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 5
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 claims description 5
- 239000010455 vermiculite Substances 0.000 claims description 5
- 235000019354 vermiculite Nutrition 0.000 claims description 5
- 229910052902 vermiculite Inorganic materials 0.000 claims description 5
- 235000010374 vitamin B1 Nutrition 0.000 claims description 5
- 239000011691 vitamin B1 Substances 0.000 claims description 5
- 235000019164 vitamin B2 Nutrition 0.000 claims description 5
- 239000011716 vitamin B2 Substances 0.000 claims description 5
- QYSXJUFSXHHAJI-YRZJJWOYSA-N vitamin D3 Chemical compound C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C\C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-YRZJJWOYSA-N 0.000 claims description 5
- 235000019165 vitamin E Nutrition 0.000 claims description 5
- 239000011709 vitamin E Substances 0.000 claims description 5
- 229940046009 vitamin E Drugs 0.000 claims description 5
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 claims description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 4
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 claims description 4
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 claims description 4
- 235000019155 vitamin A Nutrition 0.000 claims description 4
- 239000011719 vitamin A Substances 0.000 claims description 4
- 229940045997 vitamin a Drugs 0.000 claims description 4
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims description 3
- 150000002696 manganese Chemical class 0.000 claims description 3
- 150000003342 selenium Chemical class 0.000 claims description 3
- 150000003751 zinc Chemical class 0.000 claims description 3
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 2
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 claims description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 2
- 229910052802 copper Inorganic materials 0.000 claims description 2
- 239000010949 copper Substances 0.000 claims description 2
- 239000012467 final product Substances 0.000 claims description 2
- 229910052742 iron Inorganic materials 0.000 claims description 2
- 229910052748 manganese Inorganic materials 0.000 claims description 2
- 239000011572 manganese Substances 0.000 claims description 2
- 239000011701 zinc Substances 0.000 claims description 2
- 229910052725 zinc Inorganic materials 0.000 claims description 2
- 240000008042 Zea mays Species 0.000 abstract description 9
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 abstract description 8
- 235000005822 corn Nutrition 0.000 abstract description 8
- 206010012735 Diarrhoea Diseases 0.000 abstract description 6
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- 235000013594 poultry meat Nutrition 0.000 abstract description 2
- 239000004258 Ethoxyquin Substances 0.000 abstract 1
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- BHQCQFFYRZLCQQ-OELDTZBJSA-N cholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 BHQCQFFYRZLCQQ-OELDTZBJSA-N 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 4
- 210000000941 bile Anatomy 0.000 description 4
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- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 description 4
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- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 3
- 230000000369 enteropathogenic effect Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
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- 235000013406 prebiotics Nutrition 0.000 description 3
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Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Fodder In General (AREA)
Abstract
The present invention discloses a formula feed for chickens, comprising components of the following weight ratios: 500 to 600g/kg of puffed corn, 140 to 180g/kg of puffed and peeled soybean meal, 60 to 100g/kg of puffed soybean, 40 to 80g/kg of fermented soybean meal, 30 to 50g/kg of glucose, 30 to 50g/kg of fish meal, 20 to 40g/kg of whey powder, 10 to15g/kg of stone powder, 6 to 12g/kg of calcium hydrogen phosphate, 4.5 to 5.5g/kg of chicken compound premix, 2.5 to 3.5g/kg of compound acidifiers, 2 to 2.5g/kg of methionine, 1.5 to 2.5g/kg of salt, 0.25 to 0.75g/kg of choline chloride, 0.4 to 0.6g/kg of enterococcus faecalis, 0.15 to 0.25g/kg[of] poultry compound enzyme, 0.05 to 0.15g/kg of vitamin C, and 0.066 to 0.198g/kg of ethoxyquin. The formula feed for chickens, balanced in nutrition and high in digestibility rate, can effectively reduce a diarrhoea rate and a death rate of chickens, can facilitate the increase of weight and the growth of shin bones, and can guarantee healthy and rapid growth of chickens.
Description
Technical field
The invention belongs to field of animal feed, be specifically related to a kind of chick mixed feed and preparation method thereof.
Background technology
Laying hen breeding is to miniaturization, high nutritional need future development.0-14 age in days egg chick be subject to disconnected beak, immunity, cause of disease etc. stress impact, and in current breeding layer chicken, brood time is particularly brooded, and (0-14 age in days) stage does not have special feed in earlier stage, use the general chick material of 0-42 age in days, so egg chick body weight and shin bone are long often not up to standard, affect the production performance in whole cycle of laying hen.Due to chick stage body heat regulation ability, Development of Immune Organs imperfection, easy infection bacterium and disease are higher to nutritional requirement.Digestive system development imperfection, requires very high to the digestibility of raw material.Therefore chick stage is the critical period of body weight, bone, immunity, cardiovascular system, digestive system development, we are except carrying out feeding and management, alleviating stress be outward, also should ensure to provide the laying hens in brooding period 0-14 age in days stage high-quality feed in early stage of particularly brooding, especially will consider digestibility and the anti-irritability of feed.Research about 0-14 age in days egg chick mixed feed is started late, and lacks and gos deep into systematic research data both at home and abroad, and research and development of products is close to blank.
Summary of the invention
For addressing the above problem, the object of the present invention is to provide a kind of chick mixed feed and preparation method thereof.
First, the invention provides a kind of chick mixed feed, the component that it contains following weight proportioning: popcorn 500-600g/kg, expanded dehulled soybean meal 140-180g/kg, expanded soybean 60-100g/kg, fermented bean dregs 40-80g/kg, glucose 30-50g/kg, fish meal 30-50g/kg, whey powder 20-40g/kg, stone flour 10-15g/kg, calcium monohydrogen phosphate 6-12g/kg, chick compound premix 4.5-5.5g/kg, compound acidulant 2.5-3.5g/kg, methionine 2-2.5g/kg, salt 1.5-2.5g/kg, Choline Chloride 0.25-0.75g/kg, enterococcus faecalis (Enterococcus faecium) 0.4-0.6g/kg, fowl complex enzyme 0.15-0.25g/kg, vitamin C 0.05-0.15g/kg, ethoxyquinoline 0.066-0.198g/kg.
Further, the component that it contains following weight proportioning: popcorn 548g/kg, expanded dehulled soybean meal 159g/kg, expanded soybean 86g/kg, fermented bean dregs 60g/kg, glucose 40g/kg, fish meal 40g/kg, whey powder 30g/kg, stone flour 13g/kg, calcium monohydrogen phosphate 10g/kg, chick compound premix 5g/kg, compound acidulant 3g/kg, methionine 2.2g/kg, salt 1.8g/kg, Choline Chloride 0.5g/kg, enterococcus faecalis 0.5g/kg, fowl complex enzyme 0.2g/kg, vitamin C 0.1g/kg, ethoxyquinoline 0.198g/kg.
Wherein, the component that described chick compound premix contains following weight proportioning: retinol1 500～2000kIU/kg, neo dohyfral D3 500～700kIU/kg, vitamin E 4.5～5.5g/kg, prokayvit 0.5～0.7g/kg, vitamin B1 0.35～0.45g/kg, vitamin B2 3.0～3.5g/kg, pyridoxamine 0.75～0.85g/kg, cobalamin 0.003～0.005g/kg, nicotinic acid 7～9g/kg, D-VB5 calcium 3～5g/kg, biotin 0.05～0.07g/kg, mantoquita is by copper 1.5～2.0g/kg, molysite is by iron 13～14g/kg, manganese salt is by manganese 18～25g/kg, zinc salt is by zinc 15～20g/kg, selenium salt is by selenium 0.03～0.05g/kg, salt compounded of iodine is by iodine 0.2～0.4g/kg.
Wherein, mantoquita, molysite, manganese salt, zinc salt, selenium salt and salt compounded of iodine can be inorganic salts or the organic salt as livestock and poultry premix of this area routine, as copper sulphate, ferrous sulfate, zinc sulfate, manganese sulfate, sodium selenite, KI.
Wherein, described enterococcus faecalis is coated type enterococcus faecalis, and its deposit number is CGMCCNo.5092, and wherein viable count is hundred million/g of 30-50.
Wherein, in described compound acidulant, contain the component of following weight proportion: fumaric acid 22%, phosphoric acid 23%, lactic acid 6%, citric acid 27%, other is carrier vermiculite.
Described fowl is with containing AMS >=1000U/g, beta-mannase >=5000U/g, acid protease >=2200U/g, zytase >=2000U/g in complex enzyme.
Wherein, described vitamin C is coated vitamin C.
Wherein, described chick is preferably the egg chick of 0-14 age in days.
The present invention also provides the preparation method of described mixed feed, and it comprises the raw material of formula rate is mixed, and 55-60 DEG C of granulation is then broken, to obtain final product.
Technical scheme of the present invention, compared with prior art, has the following advantages:
(1) strong stress resistance of enterococcus faecalis CGMCC No.5092 of the present invention, can tolerate granulation high temperature, tolerance simulated gastric fluid, artificial cholate, therefore at process feed granulating, after animal gastrointestinal tract environment, also have a large amount of viable bacterias can enter smoothly the enteron aisle of animal, in enteron aisle, bring into play prebiotic function, improve animal immunizing power, reduce aquaculture cost;
(2) enterococcus faecalis of the present invention can effectively suppress common pathogen Escherichia coli K88 in animal intestinal, and K99 and staphylococcus aureus, can reduce diarrhea rate and the death rate;
(3) the product acid that enterococcus faecalis of the present invention can be a large amount of, can reduce the Growth and reproduction that pH in enteron aisle suppresses harmful bacteria, also contributes to digestion simultaneously.
(4) mixed feed of the present invention can improve chick body weight and the uniformity, can improve shin bone length and the uniformity.
Enterococcus faecalis of the present invention (Enterococcus faecalis) obtains for applicant separates from Radix Polygalae Crotalarioidis, and its biological property is as follows: bacterium colony rounding, and smooth surface, opaque, milky, the smooth of the edge, gram-positive bacteria; Single thalline is spherical or ellipticity.Enterococcus faecalis of the present invention is through simulated gastric fluid, bile fluid and stable on heating screening; Again through enzymatic productivity screening and bacteriostasis screening, can tolerate pH2.0,1% pepsic simulated gastric fluid, can tolerate 0.3% artificial bile fluid, can tolerate the pelleting temperature of 85 DEG C, also can suppress enteropathogenic E．Coli K88, K99 and staphylococcus aureus, there is the ability of mould in stronger product acid and inhibition dregs of beans, cotton dregs, maize straw.Enterococcus faecalis of the present invention (Enterococcus faecalis) applicant has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on July 26th, 2011, be called for short CGMCC, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, deposit number is CGMCC No.5092.
Brief description of the drawings
Figure 1 shows that enterococcus faecalis suppresses the inhibition zone picture of Escherichia coli K88, K99 and staphylococcus aureus.Wherein, A is the dull and stereotyped inhibition zone of K88; B is the dull and stereotyped inhibition zone of K99; C is the dull and stereotyped inhibition zone of staphylococcus aureus.
Figure 2 shows that the picture of enterococcus faecalis (CGMCC No.5092) cassiri.Wherein, A and B are respectively enterococcus faecalis (CGMCC No.5092) and the blank fermentation picture of 3 days; C and D are respectively enterococcus faecalis (CGMCC No.5092) and the blank fermentation picture of 7 days.
Detailed description of the invention
, it should be understood that described embodiment is only for the present invention is described, instead of limit the scope of the invention by any way more specific description the present invention by the following example.
The screening of embodiment 1 enterococcus faecalis CGMCC No.5092
1, primary dcreening operation
The each 5g of sample such as sample thief pig manure, cow dung and chicken manure, are soaked in aseptic 0.85% physiological saline of 20ml, and sample is broken up in concussion, make sample fully and contact with normal saline, 70 DEG C of thermostat water baths leave standstill 3 minutes, get supernatant, centrifugal 10 minutes of 5000rpm, supernatant discarded.Bacterial sediment after centrifugal is added in 10ml simulated gastric fluid, 37 DEG C of standing 2h, then centrifugal, collect after thalline, bacterial sediment is added in the artificial cholate of 10ml, 37 DEG C of standing 4h, collect thalline, and dilution spread is in LB culture medium.37 DEG C of incubators are cultivated after 12-24h, and bacterium colony is carried out to purifies and separates.The bacterium obtaining is preserved, and further sieve again.
The compound method of physiological saline is: 0.85% sodium chloride solution, and for subsequent use after autoclaving.
The compound method of simulated gastric fluid is: 1% pepsin, and 0.85% sodium chloride, with concentrated hydrochloric acid adjust pH to 2.0, for subsequent use after biofilter filtration sterilization.
The compound method of artificial cholate is: in liquid meat soup, add 0.3% pig cholate (analyzing pure), and for subsequent use after autoclaving.
The preparation method of LB solid medium is: peptone 10g, and yeast extract 5g, sodium chloride 10g, agar 1.5%, pH7.0, constant volume is after 1L, for subsequent use after autoclaving.
2, multiple sieve
(1) the LB culture medium temperature of the bacterium that configures, gone out is cooled to 45 DEG C, add Escherichia coli K88, K99 and the staphylococcus aureus (every milliliter of culture medium adds 1 microlitre bacterium liquid) of incubated overnight, concussion mixes, pour aseptic flat board into, after level is solidified, each flat board utilizes four Oxford cups to punch, in every Oxford cup, add 200 μ l bacterium liquid to be measured, build ware lid, carefully move to 37 DEG C of incubators, plate is just being put to leave standstill and is being cultivated.Cultivate after 20h, open ware lid, remove Oxford cup, use kind of calliper antibacterial circle diameter.
Result as shown in Figure 1, can be found out, enterococcus faecalis CGMCC No.5092 Escherichia coli and the staphylococcus aureus being always separated to.
The checking of embodiment 2 enterococcus faecalis resistance
(1) heat-resisting quantity
Carry out centrifugal collection by cultivating the enterococcus faecalis of 12h, insert in physiological saline, carry out resuspended after, sampling coating, meter viable count; Again physiological saline is heated in the water-bath of 70 DEG C after ten minutes, cooling rapidly, then carry out plate count, remaining viable count is 85.3%.
(2) tolerance of simulated gastric fluid
Get the bacterium liquid that 0.5ml cultivates 12h enterococcus faecalis, be dissolved in 4.5ml physiological saline, with colony counting method meter viable count.Get again quantitative above-mentioned bacterium liquid and add in the artificial bile salt culture-medium of 4.5ml, 37 DEG C of standing 2h, the remaining viable count of colony counting method meter, remaining viable count is 92.2%.
(3) tolerance of artificial bile fluid
Get the bacterium liquid that 0.5ml cultivates 12h enterococcus faecalis, be dissolved in 4.5ml physiological saline, with colony counting method meter viable count.Then separately get the above-mentioned bacterium liquid of 0.5ml and add in the artificial bile fluid culture medium of 4.5ml, 37 DEG C of standing 2h, the remaining viable count of the method for plate culture count meter, remaining viable count is 86.2%.
Result: above several experiments show, the resistance of the enterococcus faecalis being separated to from Radix Polygalae Crotalarioidis in the present invention is stronger, can tolerate higher temperature, tolerance simulated gastric fluid, artificial cholate, therefore uses as feed addictive, can ensure that a large amount of thalline is smoothly by the enteron aisle of animal, in enteron aisle, survive, bring into play its prebiotic function.
The prebiotic function of embodiment 3 enterococcus faecalis (CGMCC No.5092)
(1) bacteria resistance function checking
The enteropathogenic E．Coli K88 of incubated overnight, K99 and staphylococcus aureus bacterium liquid are counted with colony counting method.In Escherichia coli K88, the K99 of 3 pipe 5ml and staphylococcus aureus, add 5ml enterococcus faecalis bacterium liquid respectively.Cultivate 4h for 37 DEG C, the method for plate culture count calculates remaining Escherichia coli K88, K99 and the viable count of staphylococcus aureus.The viable count of remaining enteropathogenic E．Coli K88, K99 and staphylococcus aureus is 24.7%, 30.2% and 31.8%.
(2) checking of mould fungus inhibition in corn flour
Get the bacterium liquid 20ml of the enterococcus faecalis of cultivating 12h, evenly mix with 30g corn flour, blank assay is that 20ml sterilized water carries out homogeneous with 30g corn flour and mixes, and then at 3d, 5d, 8d and 10d, the growing state of the corn flour to two bottles of the insides and mould is observed respectively.Show by experiment, after 3 days, do not add in the corn flour of enterococcus faecalis bacterium liquid and have mould filament length to go out, and add the color of corn flour of enterococcus faecalis bacterium liquid still fresh, decompose to some extent but without the appearance of mould bacterial plaque or mould mycelia.After 7 days, mould amount reproduction in blank, and add the corn flour of enterococcus faecalis of the present invention only to have a small amount of mould that (Fig. 2) occurs.
Composition and the preparation method of embodiment 4 mixed feeds of the present invention
1, chick compound premix: vitamin A (500kIU/g) 3.6g/kg, neo dohyfral D3 (500kIU/g) 1.2g/kg, vitamin E (50%) 9.6g/kg, prokayvit (94%) 0.6g/kg, vitamin B1 (98%) 0.4g/kg, vitamin B2 (98%) 3.2g/kg, pyridoxamine (99%) 0.8g/kg, cobalamin (1%) 0.4g/kg, nicotinic acid (99%) 8g/kg, D-VB5 calcium (98%) 4g/kg, biotin (2%) 3g/kg, cupric sulfate pentahydrate 7.2g/kg, ferrous sulfate monohydrate 40g/kg, manganese sulfate monohydrate 63g/kg, monohydrate zinc sulphate 46g/kg, containing the sodium selenite 4g/kg of selenium 1%, containing the KI 30g/kg of iodine 1%, surplus is carrier zeolite powder, data in its bracket are the specification of each raw material.
2, compound acidulant: fumaric acid 22wt%, phosphoric acid 23wt%, lactic acid 6wt%, citric acid 27wt%, other is carrier vermiculite.
3, fowl complex enzyme: AMS 2000U/g, beta-mannase 5500U/g, acid protease 3000U/g, zytase 2500U/g.
4, mixed feed of the present invention is made up of the raw material of following weight proportioning: popcorn 524.1g, expanded dehulled soybean meal 180g, expanded soybean 60g, fermented bean dregs 80g, glucose 50g, fish meal 30g, whey powder 40g, stone flour 10g, calcium monohydrogen phosphate 12g, chick compound premix 4.5g prepared by step 1, step 2 is prepared compound acidulant 3.5g, methionine 2g, salt 2.5g, Choline Chloride (50%) 0.5g, enterococcus faecalis 0.4g (3,000,000,000/g), fowl prepared by step 3 complex enzyme 0.25g, vitamin C 0.15g, ethoxyquinoline (66%) 0.1g.
5, the preparation of mixed feed of the present invention: the raw material of composition step 4 is mixed, 55 DEG C of granulations, then broken, obtain broken material, be chick mixed feed.
Composition and the preparation method of embodiment 5 mixed feeds of the present invention
1, chick compound premix is made up of the raw material of following weight proportioning: vitamin A (500kIU/g) 3.6g/kg, neo dohyfral D3 (500kIU/g) 1.2g/kg, vitamin E (50%) 9.6g/kg, prokayvit (94%) 0.6g/kg, vitamin B1 (98%) 0.4g/kg, vitamin B2 (98%) 3.2g/kg, pyridoxamine (99%) 0.8g/kg, cobalamin (1%) 0.4g/kg, nicotinic acid (99%) 8g/kg, D-VB5 calcium (98%) 4g/kg, biotin (2%) 3g/kg, cupric sulfate pentahydrate 7.2g/kg, ferrous sulfate monohydrate 40g/kg, manganese sulfate monohydrate 63g/kg, monohydrate zinc sulphate 46g/kg, containing the sodium selenite 4g/kg of selenium 1%, containing the KI 30g/kg of iodine 1%, surplus is carrier zeolite powder, data in its bracket are the specification of each raw material.
2, compound acidulant: fumaric acid 22wt%, phosphoric acid 23wt%, lactic acid 6wt%, citric acid 27wt%, other is carrier vermiculite.
3, fowl complex enzyme: AMS 1500U/g, beta-mannase 5000U/g, acid protease 2500U/g, zytase 2200U/g.
4, chick mixed feed is made up of the raw material of following weight proportioning: popcorn 584.4g, expanded dehulled soybean meal 140g, expanded soybean 100g, fermented bean dregs 40g, glucose 30g, fish meal 50g, whey powder 20g, stone flour 15g, calcium monohydrogen phosphate 6g, chick compound premix 5.5g prepared by step 1, compound acidulant 2.5g, methionine 2.5g, salt 1.5g, Choline Chloride (50%) 1.5g, (5,000,000,000/g) 0.6g of enterococcus faecalis, fowl complex enzyme 0.15g, vitamin C 0.05g, ethoxyquinoline (66%) 0.3g.
5, the preparation of mixed feed of the present invention: the raw material of composition step 4 is mixed, 60 DEG C of granulations, then broken, obtain broken material, be chick mixed feed.
Composition and the preparation method of embodiment 6 mixed feeds of the present invention
1, chick compound premix is made up of the raw material of following weight proportioning: vitamin A (500kIU/g) 3.6g/kg, neo dohyfral D3 (500kIU/g) 1.2g/kg, vitamin E (50%) 9.6g/kg, prokayvit (94%) 0.6g/kg, vitamin B1 (98%) 0.4g/kg, vitamin B2 (98%) 3.2g/kg, pyridoxamine (99%) 0.8g/kg, cobalamin (1%) 0.4g/kg, nicotinic acid (99%) 8g/kg, D-VB5 calcium (98%) 4g/kg, biotin (2%) 3g/kg, cupric sulfate pentahydrate 7.2g/kg, ferrous sulfate monohydrate 40g/kg, manganese sulfate monohydrate 63g/kg, monohydrate zinc sulphate 46g/kg, containing the sodium selenite 4g/kg of selenium 1%, containing the KI 30g/kg of iodine 1%, surplus is carrier zeolite powder, data in its bracket are the specification of each raw material.
2, compound acidulant: fumaric acid 22wt%, phosphoric acid 23wt%, lactic acid 6wt%, citric acid 27wt%, other is carrier vermiculite.
3, fowl complex enzyme: AMS 1500U/g, beta-mannase 6000U/g, acid protease 3200U/g, zytase 3000U/g.
4,0-14 age in days egg chick mixed feed is made up of the raw material of following weight proportioning: popcorn 548g, expanded dehulled soybean meal 159g, expanded soybean 86g, fermented bean dregs 60g, glucose 40g, fish meal 40g, whey powder 30g, stone flour 13g, calcium monohydrogen phosphate 10g, chick compound premix 5g prepared by step 1, compound acidulant 3g, methionine 2.2g, salt 1.8g, Choline Chloride (50%) 1g, (4,000,000,000/g) 0.5g of enterococcus faecalis, fowl complex enzyme 0.2g, vitamin C 0.1g, ethoxyquinoline (66%) 0.2g.
5, the preparation of mixed feed of the present invention: the raw material of composition step 4 is mixed, 60 DEG C of granulations, then broken, obtain broken material, be chick mixed feed.
The feeding effect of test example 1 mixed feed of the present invention
1 materials and methods
1.1 test specimens: the mixed feed of the embodiment of the present invention 4～6 preparations.
1.2 test periods: 14 days～December 28 December in 2011.
1.3 experimental design:
2400 of blue brown egg chick in sea selecting 1 week age, adopt one factor factorial experiment with complete randomization design design, are divided at random 2 processed group (control group, mixed feed group of the present invention), every group of 6 repetitions, 200 chickens of each repetition.Initial body weight, the long statistical analysis of shin, group difference is remarkable (P > 0.05) not.
1.4 test daily rations: experimental group daily ration is the chick mixed feed of the embodiment of the present invention 4～6 preparations, and control group is commercially available common brood period feed for laying hen product.
1.5 feeding and management
Test chicken adopts online flat supporting, and hand fed, freely drinks water.
1.6 test determination indexs: to repeat as unit, record body weight, dead number of elements, diarrhoea number of elements, shin bone length, feed consumption rate etc., calculate average weight, average shin length, day feed consumption, the death rate, feed conversion rate etc.
1.7 statistical analysis
Use SPSS 10.0 softwares to carry out statistical analysis to test data.
2. result of the test is in table 1.
As can be seen from Table 1: test group is compared with control group, egg chick 14 age in days body weight improve 5.4～8.1%, per day feed consumption rate has reduced by 5.8～7.6%, feedstuff-meat ratio has reduced by 13.0～13.5%, 14 age in days shin length have increased by 4.8～9.5%, diarrhea rate has reduced by 54.8～65.2%, and the death rate has reduced by 61.0～76.83%.
3 conclusions
Result of the test shows that chick mixed feed of the present invention is balanced in nutrition, digestibility is high, can effectively reduce 0-14 age in days egg chick diarrhea rate and the death rate, is conducive to the increase of body weight and the growth of shin bone, can ensure the healthy Fast Growth of chick.
The impact of table 1 mixed feed of the present invention on 0-14 age in days egg chick production performance
| Project | Control group | Embodiment 4 | Embodiment 5 | Embodiment 6 | P value |
| 14 age in days body weight (g) | 111 | 117 | 116 | 120 | P＜0.05 |
| Per day feed consumption rate (g) | 172 | 162 | 164 | 159 | P＜0.05 |
| Feedstuff-meat ratio | 2.30∶1 | 1.99∶1 | 1.20∶1 | 1.99∶1 | P＜0.05 |
| 14 age in days shins long (mm) | 42 | 44 | 45 | 46 | P＜0.05 |
| Diarrhea rate (%) | 6.95 | 2.98 | 3.14 | 2.42 | P＜0.05 |
| The death rate (%) | 0.82 | 0.32 | 0.25 | 0.19 | P＜0.05 |
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, do not departing under the prerequisite of the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (5)
1. a 0-14 age in days egg chick mixed feed, it is characterized in that, component by following weight proportioning forms: popcorn 500-600g/kg, expanded dehulled soybean meal 140-180g/kg, expanded soybean 60-100g/kg, fermented bean dregs 40-80g/kg, glucose 30-50g/kg, fish meal 30-50g/kg, whey powder 20-40g/kg, stone flour 10-15g/kg, calcium monohydrogen phosphate 6-12g/kg, chick compound premix 4.5-5.5g/kg, compound acidulant 2.5-3.5g/kg, methionine 2-2.5g/kg, salt 1.5-2.5g/kg, Choline Chloride 0.25-0.75g/kg, enterococcus faecalis 0.4-0.6g/kg, fowl complex enzyme 0.15-0.25g/kg, vitamin C 0.05-0.15g/kg, ethoxyquinoline 0.066-0.198g/kg, described enterococcus faecalis is coated type enterococcus faecalis, and its deposit number is CGMCC No.5092, and wherein viable count is hundred million/g of 30-50, in described compound acidulant, contain the component of following weight proportion: fumaric acid 22%, phosphoric acid 23%, lactic acid 6%, citric acid 27%, other is carrier vermiculite, described fowl, with in complex enzyme, contains AMS >=1000U/g, 'beta '-mannase >=5000U/g, acid protease >=2200U/g, zytase >=2000U/g.
2. mixed feed as claimed in claim 1, it is characterized in that, component by following weight proportioning forms: popcorn 548g/kg, expanded dehulled soybean meal 159g/kg, expanded soybean 86g/kg, fermented bean dregs 60g/kg, glucose 40g/kg, fish meal 40g/kg, whey powder 30g/kg, stone flour 13g/kg, calcium monohydrogen phosphate 10g/kg, chick compound premix 5g/kg, compound acidulant 3g/kg, methionine 2.2g/kg, salt 1.8g/kg, Choline Chloride 0.5g/kg, enterococcus faecalis 0.5g/kg, fowl complex enzyme 0.2g/kg, vitamin C 0.1g/kg, ethoxyquinoline 0.132g/kg.
3. mixed feed as claimed in claim 1 or 2, it is characterized in that, the component that described chick compound premix contains following weight proportioning: vitamin A 1500～2000kIU/kg, neo dohyfral D3 500～700kIU/kg, vitamin E 4.5～5.5g/kg, prokayvit 0.5～0.7g/kg, vitamin B1 0.35～0.45g/kg, vitamin B2 3.0～3.5g/kg, pyridoxamine 0.75～0.85g/kg, cobalamin 0.003～0.005g/kg, nicotinic acid 7～9g/kg, D-VB5 calcium 3～5g/kg, biotin 0.05～0.07g/kg, mantoquita is by copper 1.5～2.0g/kg, molysite is by iron 13～14g/kg, manganese salt is by manganese 18～25g/kg, zinc salt is by zinc 15～20g/kg, selenium salt is by selenium 0.03～0.05g/kg, salt compounded of iodine is by iodine 0.2～0.4g/kg.
4. mixed feed as claimed in claim 1 or 2, is characterized in that, described vitamin C is coated vitamin C.
5. the preparation method of the mixed feed described in claim 1～4 any one, it comprises the raw material of formula rate is mixed, 55-60 DEG C of granulation is then broken, to obtain final product.
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| CN103074281A (en) * | 2013-01-21 | 2013-05-01 | 黑龙江八一农垦大学 | Enterococcus faecalis FJL19 and application thereof |
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| CN115011508B (en) * | 2022-05-05 | 2024-02-09 | 国家粮食和物资储备局科学研究院 | Enterococcus faecium, its microcapsule preparation and preparation method |
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| CN102293339A (en) * | 2011-08-17 | 2011-12-28 | 北京大北农科技集团股份有限公司 | Additive premix for laying hens |
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2012
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| US6156355A (en) * | 1998-11-02 | 2000-12-05 | Star-Kist Foods, Inc. | Breed-specific canine food formulations |
| CN1771831A (en) * | 2004-11-12 | 2006-05-17 | 浙江大舜恒益生物技术有限公司 | Feed-grade microbial additive and preparation and application thereof |
| CN101971924A (en) * | 2010-09-06 | 2011-02-16 | 济南和美华动物营养品有限公司 | Layer brood-time starter feed |
| CN102031235A (en) * | 2010-11-09 | 2011-04-27 | 中国农业大学 | Enterococcus faecium ANSE228 and application thereof |
| CN102293339A (en) * | 2011-08-17 | 2011-12-28 | 北京大北农科技集团股份有限公司 | Additive premix for laying hens |
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