CN107484879A - Feed addictive, antibiotic-free feed and its application - Google Patents
Feed addictive, antibiotic-free feed and its application Download PDFInfo
- Publication number
- CN107484879A CN107484879A CN201710775139.7A CN201710775139A CN107484879A CN 107484879 A CN107484879 A CN 107484879A CN 201710775139 A CN201710775139 A CN 201710775139A CN 107484879 A CN107484879 A CN 107484879A
- Authority
- CN
- China
- Prior art keywords
- feed
- parts
- antibiotic
- feed addictive
- acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000843 powder Substances 0.000 claims abstract description 37
- 239000000203 mixture Substances 0.000 claims abstract description 34
- 102000004190 Enzymes Human genes 0.000 claims abstract description 31
- 108090000790 Enzymes Proteins 0.000 claims abstract description 31
- 239000000341 volatile oil Substances 0.000 claims abstract description 25
- 238000009472 formulation Methods 0.000 claims abstract description 22
- 239000006041 probiotic Substances 0.000 claims abstract description 21
- 235000018291 probiotics Nutrition 0.000 claims abstract description 21
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 54
- 239000000463 material Substances 0.000 claims description 47
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 42
- 230000003115 biocidal effect Effects 0.000 claims description 34
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 34
- 229940088598 enzyme Drugs 0.000 claims description 30
- 241000196324 Embryophyta Species 0.000 claims description 24
- MGSRCZKZVOBKFT-UHFFFAOYSA-N thymol Chemical compound CC(C)C1=CC=C(C)C=C1O MGSRCZKZVOBKFT-UHFFFAOYSA-N 0.000 claims description 24
- 241000588724 Escherichia coli Species 0.000 claims description 21
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 21
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 21
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 21
- 102000004169 proteins and genes Human genes 0.000 claims description 20
- 108090000623 proteins and genes Proteins 0.000 claims description 20
- 210000002969 egg yolk Anatomy 0.000 claims description 19
- 241000193468 Clostridium perfringens Species 0.000 claims description 18
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 18
- 235000010755 mineral Nutrition 0.000 claims description 18
- 239000011707 mineral Substances 0.000 claims description 18
- 239000004310 lactic acid Substances 0.000 claims description 17
- 235000014655 lactic acid Nutrition 0.000 claims description 17
- 235000013312 flour Nutrition 0.000 claims description 16
- 108010011619 6-Phytase Proteins 0.000 claims description 14
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 14
- 102000004139 alpha-Amylases Human genes 0.000 claims description 14
- 108090000637 alpha-Amylases Proteins 0.000 claims description 14
- 229940024171 alpha-amylase Drugs 0.000 claims description 14
- 229940085127 phytase Drugs 0.000 claims description 14
- 229940088594 vitamin Drugs 0.000 claims description 14
- 229930003231 vitamin Natural products 0.000 claims description 14
- 235000013343 vitamin Nutrition 0.000 claims description 14
- 239000011782 vitamin Substances 0.000 claims description 14
- WBYWAXJHAXSJNI-VOTSOKGWSA-M .beta-Phenylacrylic acid Natural products [O-]C(=O)\C=C\C1=CC=CC=C1 WBYWAXJHAXSJNI-VOTSOKGWSA-M 0.000 claims description 13
- WBYWAXJHAXSJNI-SREVYHEPSA-N Cinnamic acid Chemical compound OC(=O)\C=C/C1=CC=CC=C1 WBYWAXJHAXSJNI-SREVYHEPSA-N 0.000 claims description 13
- 235000013985 cinnamic acid Nutrition 0.000 claims description 13
- 229930016911 cinnamic acid Natural products 0.000 claims description 13
- WBYWAXJHAXSJNI-UHFFFAOYSA-N methyl p-hydroxycinnamate Natural products OC(=O)C=CC1=CC=CC=C1 WBYWAXJHAXSJNI-UHFFFAOYSA-N 0.000 claims description 13
- 239000005844 Thymol Substances 0.000 claims description 12
- 229960000790 thymol Drugs 0.000 claims description 12
- 239000003112 inhibitor Substances 0.000 claims description 11
- -1 compound vitamin Chemical class 0.000 claims description 9
- 235000019733 Fish meal Nutrition 0.000 claims description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims description 8
- 239000004467 fishmeal Substances 0.000 claims description 8
- 239000008103 glucose Substances 0.000 claims description 8
- 235000019691 monocalcium phosphate Nutrition 0.000 claims description 8
- 239000004575 stone Substances 0.000 claims description 8
- 239000005862 Whey Substances 0.000 claims description 7
- 102000007544 Whey Proteins Human genes 0.000 claims description 7
- 108010046377 Whey Proteins Proteins 0.000 claims description 7
- 240000008042 Zea mays Species 0.000 claims description 7
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 7
- 241000482268 Zea mays subsp. mays Species 0.000 claims description 7
- 235000005822 corn Nutrition 0.000 claims description 7
- 230000001580 bacterial effect Effects 0.000 claims description 6
- 150000001413 amino acids Chemical class 0.000 claims description 5
- 229940062672 calcium dihydrogen phosphate Drugs 0.000 claims description 4
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 235000015097 nutrients Nutrition 0.000 claims description 3
- 239000011573 trace mineral Substances 0.000 claims description 3
- 235000013619 trace mineral Nutrition 0.000 claims description 3
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 2
- 239000013589 supplement Substances 0.000 claims description 2
- 244000025254 Cannabis sativa Species 0.000 claims 1
- 244000068988 Glycine max Species 0.000 claims 1
- 235000010469 Glycine max Nutrition 0.000 claims 1
- 239000003921 oil Substances 0.000 claims 1
- 239000012138 yeast extract Substances 0.000 abstract description 8
- 229940041514 candida albicans extract Drugs 0.000 abstract description 7
- 230000036039 immunity Effects 0.000 abstract description 6
- 235000021050 feed intake Nutrition 0.000 abstract description 4
- 238000012360 testing method Methods 0.000 description 36
- 230000000052 comparative effect Effects 0.000 description 34
- 238000002474 experimental method Methods 0.000 description 27
- 244000144972 livestock Species 0.000 description 27
- 244000144977 poultry Species 0.000 description 27
- 230000000694 effects Effects 0.000 description 18
- 230000037396 body weight Effects 0.000 description 16
- 235000018102 proteins Nutrition 0.000 description 16
- 244000052616 bacterial pathogen Species 0.000 description 14
- 241001465754 Metazoa Species 0.000 description 13
- 108091005804 Peptidases Proteins 0.000 description 13
- 239000004365 Protease Substances 0.000 description 13
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 13
- 230000036541 health Effects 0.000 description 12
- 238000004519 manufacturing process Methods 0.000 description 12
- 210000002784 stomach Anatomy 0.000 description 11
- 244000063299 Bacillus subtilis Species 0.000 description 10
- 235000014469 Bacillus subtilis Nutrition 0.000 description 10
- 206010012735 Diarrhoea Diseases 0.000 description 10
- 230000029087 digestion Effects 0.000 description 10
- 241000894006 Bacteria Species 0.000 description 9
- 238000006243 chemical reaction Methods 0.000 description 9
- 201000010099 disease Diseases 0.000 description 9
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 9
- 230000001629 suppression Effects 0.000 description 9
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 8
- GHOKWGTUZJEAQD-ZETCQYMHSA-N (D)-(+)-Pantothenic acid Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-ZETCQYMHSA-N 0.000 description 8
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 8
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 8
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 8
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 8
- 239000003814 drug Substances 0.000 description 8
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 8
- 235000013305 food Nutrition 0.000 description 8
- 241000588722 Escherichia Species 0.000 description 7
- 239000002131 composite material Substances 0.000 description 7
- 230000000688 enterotoxigenic effect Effects 0.000 description 7
- 210000000936 intestine Anatomy 0.000 description 7
- 244000000010 microbial pathogen Species 0.000 description 7
- 235000012424 soybean oil Nutrition 0.000 description 7
- 239000003549 soybean oil Substances 0.000 description 7
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 6
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 6
- 230000009471 action Effects 0.000 description 6
- 150000001875 compounds Chemical class 0.000 description 6
- 235000018977 lysine Nutrition 0.000 description 6
- 229930182817 methionine Natural products 0.000 description 6
- 235000006109 methionine Nutrition 0.000 description 6
- 235000016709 nutrition Nutrition 0.000 description 6
- 230000035764 nutrition Effects 0.000 description 6
- 238000012545 processing Methods 0.000 description 6
- 235000014590 basal diet Nutrition 0.000 description 5
- 229940079593 drug Drugs 0.000 description 5
- 238000013401 experimental design Methods 0.000 description 5
- 230000003031 feeding effect Effects 0.000 description 5
- 239000002994 raw material Substances 0.000 description 5
- 235000008521 threonine Nutrition 0.000 description 5
- 238000012546 transfer Methods 0.000 description 5
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 4
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 4
- 208000003643 Callosities Diseases 0.000 description 4
- GHOKWGTUZJEAQD-UHFFFAOYSA-N Chick antidermatitis factor Natural products OCC(C)(C)C(O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-UHFFFAOYSA-N 0.000 description 4
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 4
- 101710146739 Enterotoxin Proteins 0.000 description 4
- 206010020649 Hyperkeratosis Diseases 0.000 description 4
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 4
- TURHTASYUMWZCC-UHFFFAOYSA-N Olaquindox [BAN:INN] Chemical compound C1=CC=C2N([O-])C(C)=C(C(=O)NCCO)[N+](=O)C2=C1 TURHTASYUMWZCC-UHFFFAOYSA-N 0.000 description 4
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 4
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 4
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 4
- 229930003427 Vitamin E Natural products 0.000 description 4
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 4
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 4
- 235000001014 amino acid Nutrition 0.000 description 4
- 230000002924 anti-infective effect Effects 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 239000011616 biotin Substances 0.000 description 4
- 229960002685 biotin Drugs 0.000 description 4
- 235000020958 biotin Nutrition 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 229910052802 copper Inorganic materials 0.000 description 4
- 239000010949 copper Substances 0.000 description 4
- 239000000147 enterotoxin Substances 0.000 description 4
- 231100000655 enterotoxin Toxicity 0.000 description 4
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 229960000304 folic acid Drugs 0.000 description 4
- 235000019152 folic acid Nutrition 0.000 description 4
- 239000011724 folic acid Substances 0.000 description 4
- 230000037406 food intake Effects 0.000 description 4
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 4
- 229910052740 iodine Inorganic materials 0.000 description 4
- 239000011630 iodine Substances 0.000 description 4
- 229910052742 iron Inorganic materials 0.000 description 4
- 229950007634 kitasamycin Drugs 0.000 description 4
- XYJOGTQLTFNMQG-KJHBSLKPSA-N leucomycin V Chemical compound CO[C@H]1[C@H](O)CC(=O)O[C@H](C)C\C=C\C=C\[C@H](O)[C@H](C)C[C@H](CC=O)[C@@H]1O[C@H]1[C@H](O)[C@@H](N(C)C)[C@H](O[C@@H]2O[C@@H](C)[C@H](O)[C@](C)(O)C2)[C@@H](C)O1 XYJOGTQLTFNMQG-KJHBSLKPSA-N 0.000 description 4
- 150000002669 lysines Chemical class 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000000813 microbial effect Effects 0.000 description 4
- 235000001968 nicotinic acid Nutrition 0.000 description 4
- 229960003512 nicotinic acid Drugs 0.000 description 4
- 239000011664 nicotinic acid Substances 0.000 description 4
- 229950010210 olaquindox Drugs 0.000 description 4
- 229940055726 pantothenic acid Drugs 0.000 description 4
- 235000019161 pantothenic acid Nutrition 0.000 description 4
- 239000011713 pantothenic acid Substances 0.000 description 4
- 235000019260 propionic acid Nutrition 0.000 description 4
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- 239000011669 selenium Substances 0.000 description 4
- 229910052711 selenium Inorganic materials 0.000 description 4
- 150000003588 threonines Chemical class 0.000 description 4
- 235000019155 vitamin A Nutrition 0.000 description 4
- 239000011719 vitamin A Substances 0.000 description 4
- 239000011715 vitamin B12 Substances 0.000 description 4
- 239000011726 vitamin B6 Substances 0.000 description 4
- 235000019165 vitamin E Nutrition 0.000 description 4
- 229940046009 vitamin E Drugs 0.000 description 4
- 239000011709 vitamin E Substances 0.000 description 4
- 239000011652 vitamin K3 Substances 0.000 description 4
- 229940045997 vitamin a Drugs 0.000 description 4
- 239000011701 zinc Substances 0.000 description 4
- 229910052725 zinc Inorganic materials 0.000 description 4
- 239000001763 2-hydroxyethyl(trimethyl)azanium Substances 0.000 description 3
- 235000019743 Choline chloride Nutrition 0.000 description 3
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- SGMZJAMFUVOLNK-UHFFFAOYSA-M choline chloride Chemical compound [Cl-].C[N+](C)(C)CCO SGMZJAMFUVOLNK-UHFFFAOYSA-M 0.000 description 3
- 229960003178 choline chloride Drugs 0.000 description 3
- 235000019621 digestibility Nutrition 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 210000002429 large intestine Anatomy 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 235000019192 riboflavin Nutrition 0.000 description 3
- 229960002477 riboflavin Drugs 0.000 description 3
- 239000002151 riboflavin Substances 0.000 description 3
- 239000011691 vitamin B1 Substances 0.000 description 3
- 239000011647 vitamin D3 Substances 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 235000005979 Citrus limon Nutrition 0.000 description 2
- 244000131522 Citrus pyriformis Species 0.000 description 2
- 208000004232 Enteritis Diseases 0.000 description 2
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
- 239000004472 Lysine Substances 0.000 description 2
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 2
- MJVAVZPDRWSRRC-UHFFFAOYSA-N Menadione Chemical compound C1=CC=C2C(=O)C(C)=CC(=O)C2=C1 MJVAVZPDRWSRRC-UHFFFAOYSA-N 0.000 description 2
- 241000607142 Salmonella Species 0.000 description 2
- 241000282887 Suidae Species 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- AGVAZMGAQJOSFJ-WZHZPDAFSA-M cobalt(2+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+2].N#[C-].[N-]([C@@H]1[C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP(O)(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O AGVAZMGAQJOSFJ-WZHZPDAFSA-M 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 235000013601 eggs Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 206010016766 flatulence Diseases 0.000 description 2
- 210000001035 gastrointestinal tract Anatomy 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000002757 inflammatory effect Effects 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 229910052748 manganese Inorganic materials 0.000 description 2
- 239000011572 manganese Substances 0.000 description 2
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 2
- 239000002366 mineral element Substances 0.000 description 2
- 230000001338 necrotic effect Effects 0.000 description 2
- 235000002949 phytic acid Nutrition 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- ZUFQODAHGAHPFQ-UHFFFAOYSA-N pyridoxine hydrochloride Chemical compound Cl.CC1=NC=C(CO)C(CO)=C1O ZUFQODAHGAHPFQ-UHFFFAOYSA-N 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- QYSXJUFSXHHAJI-YRZJJWOYSA-N vitamin D3 Chemical compound C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C\C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-YRZJJWOYSA-N 0.000 description 2
- 210000005253 yeast cell Anatomy 0.000 description 2
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 description 1
- 241000272525 Anas platyrhynchos Species 0.000 description 1
- 241000272517 Anseriformes Species 0.000 description 1
- 239000005711 Benzoic acid Substances 0.000 description 1
- 241000522254 Cassia Species 0.000 description 1
- 241000193403 Clostridium Species 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 1
- 231100000678 Mycotoxin Toxicity 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 206010042434 Sudden death Diseases 0.000 description 1
- 241000282898 Sus scrofa Species 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- 229930003316 Vitamin D Natural products 0.000 description 1
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000001142 anti-diarrhea Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 235000010233 benzoic acid Nutrition 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 238000005660 chlorination reaction Methods 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 208000001848 dysentery Diseases 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 230000000369 enteropathogenic effect Effects 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000007413 intestinal health Effects 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002636 mycotoxin Substances 0.000 description 1
- 230000000926 neurological effect Effects 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000000467 phytic acid Substances 0.000 description 1
- 229940068041 phytic acid Drugs 0.000 description 1
- 239000004302 potassium sorbate Substances 0.000 description 1
- 235000010241 potassium sorbate Nutrition 0.000 description 1
- 229940069338 potassium sorbate Drugs 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 208000026775 severe diarrhea Diseases 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 235000019166 vitamin D Nutrition 0.000 description 1
- 239000011710 vitamin D Substances 0.000 description 1
- 150000003710 vitamin D derivatives Chemical class 0.000 description 1
- 229940046008 vitamin d Drugs 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/22—Compounds of alkali metals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Inorganic Chemistry (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Marine Sciences & Fisheries (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Fodder In General (AREA)
Abstract
The present invention relates to a kind of feed addictive, antibiotic-free feed and its application.The feed addictive includes 1.0 parts~1.5 parts of 0.02 part~0.04 part of probiotics, 0.1 part~0.3 part of egg powder, 0.02 part~0.05 part of plants essential oil, 0.50 part~1.00 parts of organic acidulant, 0.10 part~0.15 part of complex enzyme formulation and yeast extract according to the mass fraction.Each component cooperates, and plays synergy.After antibiotic-free feed feeding a period of time containing above-mentioned feed addictive, the immunity of child care pig is improved, and in the case where feed intake is essentially identical, eats the child care pig average daily gain containing the feed addictive apparently higher than control group.
Description
Technical field
The present invention relates to field of feed, more particularly to a kind of feed addictive, antibiotic-free feed and its application.
Background technology
Child care pig is commonly called as weanling pig, and it has the characteristics that to grow fast, high, it is necessary to feed meticulously to the neurological susceptibility of disease
Support.Child care pig respectively organizes allelotaxis not yet ripe, easily infectious disease, causes diarrhoea even dead.In the daily ration of child care pig
Middle a certain amount of antibiotic of addition can effectively prevent or control the diarrhoea of child care pig, improve the general level of the health of child care pig.
As people's living standard improves, people not only rise rapidly to the demand of animal products, to its quality and peace
The requirement of full property also more and more higher.2015 new《Environmental law》、《The law of food safety》And《Feeding quality safety management specification》
Successive appearance, all indicate the attention degree of the food-safe problem in China to a new height, following poultry
Animal husbandry will set foot on Sustainable Development Road.In the case where such overall situation is driven, feed, cultivation industry are following to realize limit comprehensively
It will be trend of the times to resist, prohibit anti-.
In existing feed research, researcher continuously attempts to substitute the antibiosis in feed completely or partially with additive
Element, but all it is difficult to the feeding effect that antibiotic is fully achieved.In addition, after using traditional feed feeding a period of time, livestock and poultry
Feed intake is more but weightening is less, and feed conversion rate is relatively low, and production cost is high, and economic benefit is poor.
The content of the invention
Based on this, it is necessary to a kind of antibiotic that can be substituted in feed is provided and can effectively improve feed conversion rate,
Reduce the feed addictive of production cost.
In addition, also provide a kind of antibiotic-free feed and its application.
A kind of feed addictive, it is characterised in that according to the mass fraction, the feed addictive includes：
The bacterial strain of C.perfringens can be suppressed or kill by containing in the probiotics, contain large intestine in the egg powder
Bacillus special yolk antibody, the potency of Escherichia coli special yolk antibody described in the egg powder be 5000IU~
8000IU。
In one embodiment, the active component in the probiotics is bacillus subtilis PB6.
In one embodiment, the plants essential oil is selected from least one of cinnamic acid and thymol.
In one embodiment, the organic acidulant includes lactic acid, citric acid and phosphoric acid, the lactic acid, the lemon
The mass ratio of the sour and described phosphoric acid of lemon is 1~3:2~4:4~6.
In one embodiment, the complex enzyme formulation includes phytase, protease, alpha-amylase and beta-mannase
Enzyme.
It is acceptable auxiliary on a kind of antibiotic-free feed, including feed addictive described in any of the above-described and feed science
Material.
In one embodiment, the feed addictive accounts for the weight/mass percentage composition of the antibiotic-free feed
0.5%~5%.
In one embodiment, according to the mass fraction, the auxiliary material includes：
80 parts~99 parts of energy and protein material；
1 part~5 parts of premix；And
0.1 part~0.5 part of mould inhibitor；
Wherein, the energy and protein material are used to provide basic energy and protein requirement, and the premix is used
In providing trace element and supplementing the nutrients, the mould inhibitor is used to prevent the feed from going mouldy.
In one embodiment, the energy and protein material include 30 parts~60 parts of jade according to mass fraction meter
Rice, 0.01 part~15 parts of popcorn, 5 parts~10 parts of flour, 2 parts~5 portions soybean oils, 16 parts~22 parts of dregs of beans, 2.5
Part~7.5 parts of fermented bean dregs, 2 parts~4 parts fish meal, 0.01 part~5 portions whey powders and 2.5 parts~5 parts glucose；And/or
The premix include 0.3 part~0.9 part amino acid, 1 part~1.4 parts of calcium dihydrogen phosphate, 0.50 part~
1.00 parts of stone flour, 0.3 part~0.5 part of sodium chloride and 0.1 part~0.2 part of the more mineral matters of compound vitamin.
Feed addictive described in any of the above-described or the as described above antibiotic-free feed described in any one are raised in child care pig
Application in material.
Above-mentioned feed addictive includes probiotics, egg powder, plants essential oil, organic acidulant, complex enzyme formulation and ferment
Female extract., can specificity kill perfringens shuttle containing the bacterial strain that can suppress or kill C.perfringens in probiotics
Bacterium, while suppress the growth of some bacteriums such as Escherichia coli, salmonella, maintain microbial population of animal intestinal tract balance.Containing Escherichia coli
The egg powder of special yolk antibody, it can effectively prevent and reduce coliform diarrhea.Plants essential oil has broad-spectrum antibacterial
Effect, there is certain inhibitory action to most pathogenic bacteria.Organic acidulant plays pH value adjustment effect in stomach, improves livestock and poultry
To the digestion power of nutriment, bacteriostasis is played in enteron aisle, suppresses harmful pathogenic microorganism breeding.Complex enzyme formulation can
Improve utilization ratio of the livestock and poultry to basal diet.Yeast extract enables to the taste of feed more preferably, and improves exempting from for livestock and poultry
Epidemic disease power.Probiotics, egg powder, plants essential oil, organic acidulant, complex enzyme formulation and yeast in above-mentioned feed addictive carry
Take thing to cooperate, play synergy.Above-mentioned feed addictive has the advantages that：(1) specific can suppress
The growth of C.perfringens and enterotoxigenic escherichia coil, and the growth of the most of pathogenic bacteria of suppression of broad spectrum activity, prevent
Pathogenic microorganism amount reproduction in stomach and enteron aisle is only harmful to, maintains colony balance in animal and bird intestines, improves livestock and poultry to causing
The anti-infection ability of germ；(2) improve the taste of feed, improve digestibility and utilization efficiency of the livestock and poultry to energy feedstuff, reduce more than needed
Nutrition reaches enteron aisle back segment and carries out bad fermentation；(3) each materials safety, the use without the resistance to the action of a drug, effectively reduction antibiotic, are reduced
Antibiotic residue in livestock and poultry bodies；(4) immunity of livestock of raising is improved, and is improved food conversion ratio, is reduced and be produced into
This.Test result indicates that after using antibiotic-free feed feeding a period of time containing above-mentioned feed addictive, child care pig is exempted from
Epidemic disease power is improved, and in the case where feed intake is essentially identical, eats the child care pig average daily gain containing the feed addictive
Apparently higher than control group.
Embodiment
In order to facilitate the understanding of the purposes, features and advantages of the present invention, with reference to specific embodiment pair
The embodiment of the present invention is described in detail.Many details are elaborated in the following description in order to fully manage
The solution present invention.But the invention can be embodied in many other ways as described herein, those skilled in the art can
To do similar improvement in the case of without prejudice to intension of the present invention, therefore the present invention is not limited by following public specific implementation
System.
Unless otherwise defined, all of technologies and scientific terms used here by the article is with belonging to technical field of the invention
The implication that technical staff is generally understood that is identical.Term used in the description of the invention herein is intended merely to description tool
The purpose of the embodiment of body, it is not intended that in the limitation present invention.
In the feed addictive of an embodiment, according to the mass fraction, the feed addictive includes：
Wherein, the bacterial strain of C.perfringens can be suppressed or kill by containing in probiotics, contain large intestine bar in egg powder
Bacterium special yolk antibody, the potency of Escherichia coli special yolk antibody is 5000IU~8000IU.
Inventor has found that under normal circumstances, C.perfringens is that the bacterium that lives away from home of animal intestinal tract is general in research process
It will not cause a disease.But sanitary condition is poor, feeding management is bad and wean, turn the stressed conditions such as group under, the enteron aisle inner ring of swinery
Border is changed, and normal intestinal flora is destroyed, then may cause C.perfringens amount reproduction, and produce toxin and cause
Morbidity.Harm of the C.perfringens to pig industry runs through the whole all one's life of pig, can cause red dysentery, the clostridium property piglet abdomen of sucking pig
Rush down, in big pig and sow the symptom such as flatulence, necrotic enteritis and sudden death.Added in above-mentioned feed addictive and contain and can suppress
Or the probiotics of the bacterial strain of C.perfringens is killed, suppress or kill C.perfringens in the nursing stage of child care pig, protect
Hold colony balance, pre- anti-diarrhea, flatulence etc. in child care chitling road.
Specifically, the active component in probiotics is bacillus subtilis PB6.In the child care pig stage, c-type perfringens shuttle
Bacterium is multiple, easily causes piglet necrotic enteritis.Bacillus subtilis PB6 being capable of specificity suppression c-type C.perfringens cause
Disease, improve child care pig health.Meanwhile bacillus subtilis PB6 can also suppress some bacteriums such as Escherichia coli, salmonella
Growth, maintain colony balance in child care chitling road.
Specifically, bacillus subtilis PB6 concentration is the CFU/g of 4,000,000,000 CFU/g~6,000,000,000 in probiotics.
In addition, grice diarrhoea is one of main bugbear for perplexing large scale of pig farm production, brought to pig industry huge
Economic loss.Enteropathogenic E. Coli is the most important cause of disease for causing newborn piglet and weanling pig, wherein production enterotoxin
Escherichia coli (ETEC) are most important pathogenic bacteria.Suppress production enterotoxin large intestine bar by adding antibiotic in traditional feed
The growth of bacterium.The egg powder containing Escherichia coli special yolk antibody is added in above-mentioned feed addictive, passes through Escherichia coli
Special yolk antibody can effectively prevent and reduce coliform diarrhea, prevent and reduce grice diarrhoea, play portion
Divide the use of replacement or replacing whole antibiotic.
In one embodiment, the egg powder containing Escherichia coli special yolk antibody is prepared by the following procedure method and obtained
：By the hen of ETEC antigen immunes, make to produce corresponding ETEC specific antibodies in hen body, and shift, be stored in ovum
Huang Zhong.The yolk for the egg that hen after immune is produced is dry, crushes, and obtains containing Escherichia coli special yolk antibody
Egg powder.Specifically, during the hen of ETEC antigen immunes, immune number is multiple, such as twice, three times or five is inferior.
By being repeatedly immunized so that corresponding ETEC specific antibodies are produced in hen body, and shifts, be stored in yolk, antibody
Potency it is high, so as to effectively suppress or kill enterotoxigenic escherichia coil.
Specifically, the potency of Escherichia coli special yolk antibody is 5000IU~8000IU.
The bacterial strain of C.perfringens can be suppressed or kill by containing in probiotics, special containing Escherichia coli in egg powder
Property Yolk antibody.Probiotics in feed addictive is compounded with egg powder by certain mass number, effectively suppresses perfringens shuttle
Bacterium and the growth of enterotoxigenic escherichia coil, maintain colony balance in animal and bird intestines.Probiotics and egg powder have safety,
Without the resistance to the action of a drug and the characteristics of medicament residue, suppress C.perfringens, production enterotoxin improving animal health, particularly specificity
Property Escherichia coli cause a disease the alternative antibiotic application of aspect.
Specifically, a certain amount of plants essential oil is added in feed addictive, broad-spectrum antibacterial effect can be played, to majority
Pathogenic bacteria have certain inhibitory action.
In one embodiment, plants essential oil is selected from least one of cinnamic acid and thymol.
In the present embodiment, cinnamic acid and thymol are contained in plants essential oil, the mass ratio of cinnamic acid and thymol is
0.5~2：1.Find the plants essential oil containing cinnamic acid and thymol to most pathogenic bacteria in pig industry such as gold through bacteriostatic test
Staphylococcus aureus etc. have good inhibitory action, can realize bacteria resistance function by substitute antibiotics to a certain extent.
Specifically, plants essential oil can directly buy commercial goods, can also voluntarily allocate.
Plants essential oil in feed addictive is compounded with probiotics and egg powder by certain mass number, can be specific
Suppress the growth of C.perfringens and enterotoxigenic escherichia coil, and the life of the most of pathogenic bacteria of suppression of broad spectrum activity
It is long, colony balance in animal and bird intestines, prevention or treatment diarrhoea are maintained, improves the absorption efficiency of feed.Plants essential oil, probiotics and
Egg powder has safety, without the resistance to the action of a drug and the characteristics of medicament residue.
A certain amount of organic acidulant is added in feed addictive, pH value adjustment effect is played in stomach, improves livestock and poultry
To the digestion power of nutriment, bacteriostasis is played in enteron aisle, suppresses harmful pathogenic microorganism breeding.
In one embodiment, organic acidulant includes lactic acid, citric acid and phosphoric acid, lactic acid, citric acid and phosphoric acid
Mass ratio is 1~3:2~4:4~6, such as 2:3:5.Lactic acid, citric acid and being compounded by certain mass ratio for phosphoric acid are sent out in stomach
PH value adjustment effect is waved, improves digestion power of the piglet to nutriment, bacteriostasis is played in enteron aisle, it is micro- to suppress harmful cause of disease
Biological self reproducing.
The ratio such as citric acid and phosphoric acid in feed addictive is too high, easily causes the acid too strong of feed addictive,
Stomach is caused damage.And the ratio such as citric acid and phosphoric acid is too low, then it is difficult to play a part of adjusting pH value.Above-mentioned organic acid
The mass ratio of lactic acid, citric acid and phosphoric acid is 1~3 in agent:2~4:4~6, the ratio of lactic acid, citric acid and phosphoric acid is suitable,
PH value adjustment effect effectively can be played in stomach, and suppress to breed in harmful pathogenic microorganism stomach and enteron aisle.Tool
Body, phosphoric acid can be the phosphoric acid of capsule form.
Organic acidulant in feed addictive is compounded with plants essential oil, probiotics and egg powder by certain mass number,
It is capable of the growth of specific suppression C.perfringens and enterotoxigenic escherichia coil, and most of suppression of broad spectrum activity
The growth of pathogenic bacteria, maintain colony balance in animal and bird intestines.Organic acidulant further suppresses harmful pathogenic microorganism in stomach
And bred in enteron aisle so that feed digestion excellent in efficiency, safety containing the feed addictive, without the resistance to the action of a drug.
A certain amount of complex enzyme formulation is added in feed addictive can improve utilization ratio of the livestock and poultry to basal diet.
In one embodiment, complex enzyme formulation includes phytase, protease, alpha-amylase and 'beta '-mannase.
Phytase can improve the utilization ratio of phytate phosphorus, while reduce antagonism of the phytic acid to mineral element, improve mineral element
Utilization ratio.Protease and alpha-amylase can supplement child care pig endogenous enzymes hyposecretion, improve to protein raw material and energy
The digestibility and utilization efficiency of content of starting materials, reduce nutrition more than needed and reach the bad fermentation of enteron aisle back segment progress.
In addition, often contain beans material in the basal diet of pig, such as dregs of beans, gather containing substantial amounts of β-sweet dew in dregs of beans class
Sugar, beta-mannase belong to a kind of SNSP class material, are easily considered pathogen-associated molecular by immunocyte identification.If
After child care pig takes in a certain amount of beta-mannase, the immune system of body is easily activated by " mistake ", causes overreaction, draws
Play inflammatory reaction.Clinical shows as intestinal inflammatory and the growth performance caused by the utilization ratio of nutriment reduces
Lowly.'beta '-mannase in above-mentioned complex enzyme formulation prevents or alleviated child care pig due to using dregs of beans to a certain extent
Bowl inflammatory is suffered from diarrhoea caused by class.
In one embodiment, phytase, protease, alpha-amylase and the beta-mannase that complex enzyme formulation includes
Enzyme.Specifically, in the nursery-stage pig feed of preparation, phytase, protease, the enzyme activity of alpha-amylase and 'beta '-mannase are respectively
1000IU/kg~2000IU/kg, 4000IU/kg~8000IU/kg, 500IU/kg~1000IU/kg, 80000IU/kg~
320000IU/kg。
Complex enzyme formulation in feed addictive is compounded with organic acidulant by certain mass number, can effectively improve poultry
Fowl promotes food absorption, improved food conversion ratio to the digestion power of basal diet.
Further, complex enzyme formulation is compounded with probiotics and egg powder by certain mass number, specific can be pressed down
The growth of C.perfringens and enterotoxigenic escherichia coil processed, and the growth of the most of pathogenic bacteria of suppression of broad spectrum activity,
Colony balance in animal and bird intestines is maintained, keeps intestinal health, improves digestion power of the livestock and poultry to basal diet.
A certain amount of yeast extract is added in feed addictive and enables to the taste of feed more preferably, and improves livestock and poultry
Immunity.
In one embodiment, yeast extract includes yeast cell wall and yeast nucleotides.Specifically, yeast extracts
Thing is using the food yeast of rich in protein as raw material, by self-dissolving, enzymolysis, separation, concentration by the albumen in yeast cells
A kind of the brown color solubility paste or slight yellow powdery product that matter, nucleic acid etc. are prepared after being degraded.Yeast extract
Improve the taste of feed, improve the action function of nursery pig immunity.
Probiotics, egg powder, plants essential oil, organic acidulant, complex enzyme formulation and ferment in above-mentioned feed addictive
Female extract cooperates, and plays synergy.Above-mentioned feed addictive has the advantages that：(1) can be specific
Suppress the growth of C.perfringens and enterotoxigenic escherichia coil, and the life of the most of pathogenic bacteria of suppression of broad spectrum activity
It is long, harmful pathogenic microorganism amount reproduction in stomach and enteron aisle is prevented, maintains colony balance in animal and bird intestines, improves livestock and poultry
To the anti-infection ability of pathogenic bacteria；(2) improve the taste of feed, improve digestion profit of the livestock and poultry to protein raw material and energy feedstuff
With efficiency, feed digestion excellent in efficiency, reduce nutrition more than needed and reach the bad fermentation of enteron aisle back segment progress；(3) each materials safety, nonreactive
The property of medicine, the use of antibiotic is effectively reduced, reduces the antibiotic residue in livestock and poultry body；(4) immunity of livestock of raising obtains
Improve, improve food conversion ratio, reduce production cost.
Test result indicates that after feeding child care pig for a period of time using the antibiotic-free feed containing the feed addictive, protect
Educate that the average daily gain of pig is larger, and feed conversion rate is higher.In body weight and health degree with the feed efficiency phase containing antibiotic
When being even better than the feed containing antibiotic, production cost is effectively reduced, improves income.
In addition, the antibiotic-free feed of an embodiment is also provided herein.The antibiotic-free feed includes above-mentioned feed
Acceptable auxiliary material on additive and feed science.
In one embodiment, feed addictive accounts for the 0.5%~5% of the weight/mass percentage composition of antibiotic-free feed.
The ratio of feed addictive is suitable, can effectively control and improve anti-infection ability of the livestock and poultry to pathogenic bacteria, so as to substitute in feed
Antibiotic.Feed addictive can also improve the taste of feed, improve digestion of the livestock and poultry to protein raw material and energy feedstuff
Utilization ratio, improve food conversion ratio, reduce production cost.
Further, feed addictive accounts for the 1%~3% of the weight/mass percentage composition of antibiotic-free feed.
In one embodiment, auxiliary material includes energy and protein material, premix and mould inhibitor.By mass fraction
Meter, auxiliary material include 80 parts~99 parts energy and protein material, 1 part~5 parts of premix and 0.1 part~0.5 part it is mould proof
Agent.
Specifically, energy and protein material are used to provide basic energy and protein requirement for fowl poultry kind.Energy and
Protein material be selected from corn, dregs of beans, popcorn, flour, soybean oil, dregs of beans, fermented bean dregs, fish meal, whey powder and
At least one of glucose.
In the present embodiment, energy and protein material according to mass fraction meter include 30 parts~60 parts corn,
0.01 part~15 parts of popcorn, 5 parts~10 parts 2 parts~5 portions soybean oils of flour, 16 parts~22 parts of dregs of beans, 2.5 parts~
7.5 parts of fermented bean dregs, 2 parts~4 parts fish meal, 0.01 part~5 portions whey powders and 2.5 parts~5 parts glucose.Energy and protein
Material nutrition is balanced, and material is cheap, and cost is low.
Specifically, premix is used to provide trace element for fowl poultry kind and supplemented the nutrients.
In one embodiment, premix is for example including amino acid, calcium dihydrogen phosphate, stone flour, sodium chloride and compound more
Tie up more mineral matters.
Specifically, the amino acid of 0.3 part~0.9 part of premix bag, 1 part~1.4 parts of calcium dihydrogen phosphate, 0.50 part~
1.00 parts of stone flour, 0.3 part~0.5 part of sodium chloride and 0.1 part~0.2 part of the more mineral matters of compound vitamin.
Specifically, the amino acid in premix includes lysine, methionine and threonine.Lysine, methionine and Soviet Union's ammonia
The mass ratio of acid is 3~7：1~2：2~3.
Specifically, the more mineral matters of compound vitamin include composite microbial element and composite mineral matter, composite microbial element for example containing
Vitamin A, vitamin D3, vitamin E, vitamin K3, vitamin B12, riboflavin, pantothenic acid, nicotinic acid, Choline Chloride, folic acid, dimension
Raw plain B1, vitamin B6, biotin etc..Composite mineral matter is for example containing manganese, iron, zinc, copper, iodine and selenium.
In one embodiment, in the nursery-stage pig feed of preparation, the content of vitamin A for 5000IU/kg~
15000IU/kg, vitamin D3Content be 1000IU/kg~3000IU/kg, the content of vitamin E be 70IU/kg~
100IU/kg, vitamin K3Content for 2mg/kg~3mg/kg, vitamin B12Content be μ g/kg of 40 μ g/kg~60, core yellow
Element content be 5mg/kg~15mg/kg, the content of pantothenic acid is 40mg/kg~50mg/kg, the content of nicotinic acid be 40mg/kg~
50mg/kg, the content of Choline Chloride are 600mg/kg~800mg/kg, the content of folic acid is 1mg/kg~2mg/kg, vitamin
B1Content for 3mg/kg~5mg/kg, vitamin B6Content be 3mg/kg~5mg/kg, the content of biotin be 200 μ g/kg
~300 μ g/kg, the content of manganese are 30mg/kg~50mg/kg, the content of iron is 70mg/kg~90mg/kg, the content of zinc is
The content that 110mg/kg~130mg/kg, the content of copper are is 70mg/kg~90mg/kg, the content of iodine be 0.2mg/kg~
The content of 0.4mg/kg and selenium is 0.3mg/kg~0.5mg/kg.
The above-mentioned more mineral matters of compound vitamin contain the required most vitamin of fowl poultry kind growth and micro member
Element so that feed nutrition is balanced, promotes fowl poultry kind growth.
Specifically, mould inhibitor is used for the metabolism for controlling microorganism in feed and growth, the generation for suppressing mycotoxin, prevents
Feed goes mouldy.Mould inhibitor is, for example, propionic acid, potassium sorbate or benzoic acid etc..
Feed addictive in antibiotic-free feed, by compounding, cooperates with auxiliary material, plays synergy.Feed the nothing
Antibiotic residue in the fowl poultry kind body of antibiotic feed is few, and the speed of growth is fast, and the transformation efficiency of feed is high.
Antibiotic is not contained in above-mentioned antibiotic-free feed, each materials safety, without the resistance to the action of a drug reduces anti-in livestock and poultry body
Raw element residual.Feed addictive in antibiotic-free feed being capable of specific suppression C.perfringens and production enterotoxin
The growth of Escherichia coli, and the growth of the most of pathogenic bacteria of suppression of broad spectrum activity, prevent harmful pathogenic microorganism in stomach and
Amount reproduction in enteron aisle, colony balance in animal and bird intestines is maintained, improve anti-infection ability of the livestock and poultry to pathogenic bacteria.The taste of feed
Preferably, the immunity of livestock of raising is improved, and feed conversion rate is higher, reduces production cost.
Specifically, above-mentioned feed addictive or above-mentioned antibiotic-free feed can be applied in fowl poultry kinds such as pig, duck, chicken, geese
In feed.
Preferably, above-mentioned feed addictive or above-mentioned antibiotic-free feed are applied in the feed of child care pig.
Test result indicates that above-mentioned antibiotic-free feed can effectively prevent the infection of pathogenic bacteria, using the antibiotic-free
It is suitable with the feed efficiency containing antibiotic in body weight and health degree even better than to contain after feed feeds child care pig for a period of time
The feed of antibiotic.Above-mentioned antibiotic-free feed can also improve livestock and poultry and the digestibility and utilization of protein raw material and energy feedstuff is imitated
Rate, feed digestion excellent in efficiency, reduce nutrition more than needed and reach the bad fermentation of enteron aisle back segment progress, feed conversion rate is high, reduces production
Cost, have broad application prospects.
For embodiment part, (following examples unless otherwise specified, are not then contained in addition to inevitable impurity below
Other components for pointing out are not known)
Embodiment 1
Auxiliary material：By 385.1kg corns, 150kg popcorns, 100kg flour, 20kg soybean oils, 160kg dregs of beans (matter
Measure fraction for 46%), 50kg fermented bean dregs, 30kg inlet steams fish meal, 25kg whey powders, 25kg glucose, 5kg lysines,
1.3kg methionine, 2.5kg threonines, 10kg calcium dihydrogen phosphates, 10kg stone flours, 4kg salt, the more mineral matters of 1.5kg compound vitamins
And 2kg mould inhibitors (concentration is 25% propionic acid) are mixed with to form auxiliary material.Wherein the more mineral matters of compound vitamin include compound
Micro-element and composite mineral matter, composite microbial element contain vitamin A, vitamin D3, vitamin E, vitamin K3, vitamin B12、
Riboflavin, pantothenic acid, nicotinic acid, Choline Chloride, folic acid, vitamin B1, vitamin B6, biotin etc..Composite mineral matter for example containing
Manganese, iron, zinc, copper, iodine and selenium.The content of vitamin A is 10000IU/kg, vitamin D in the nursery-stage pig feed of configuration3Content
Content for 2000IU/kg, vitamin E is 90IU/kg, vitamin K3Content for 2.5mg/kg, vitamin B12Content be
50 μ g/kg, the content of riboflavin are 10mg/kg, the content of pantothenic acid is 45mg/kg, the content of nicotinic acid is 45mg/kg, chlorination courage
The content of alkali is 700mg/kg, the content of folic acid is 1.5mg/kg, vitamin B1Content for 4mg/kg, vitamin B6Content
Content for 4mg/kg, biotin is 250 μ g/kg, the content of manganese is 40mg/kg, the content of iron is 80mg/kg, the content of zinc
Content for 120mg/kg, copper is 80mg/kg, the content of iodine is 0.3mg/kg and the content of selenium is 0.5mg/kg.
0.3kg bacillus subtilis PB6,2kg egg powder are added into above-mentioned auxiliary material, and (Escherichia coli are specific in egg powder
The potency of Yolk antibody is 6000IU), 0.3kg plants essential oils, 5kg organic acidulants, 1kg complex enzyme formulations and 10kg yeast carry
Thing is taken, is uniformly mixed into the nursery-stage pig feed of antibiotic-free.Contain cinnamic acid and thymol wherein in plants essential oil, cinnamic acid and
The mass ratio of thymol is 1:1.Organic acidulant includes lactic acid, citric acid and phosphoric acid, the mass ratio of lactic acid, citric acid and phosphoric acid
For 2:3:5.Contain phytase, protease, alpha-amylase and 'beta '-mannase, the nursery-stage pig feed of configuration in complex enzyme formulation
Middle phytase, protease, the enzyme-activity unit of alpha-amylase and 'beta '-mannase be respectively 1200IU/kg, 4000IU/kg,
500IU/kg、120000IU/kg。
Embodiment 2
Auxiliary material：By 516.8kg corns, 100kg flour, 20kg soybean oils, 155kg dregs of beans (mass fraction 46%),
75kg fermented bean dregs, 20kg inlet steams fish meal, 50kg glucose, 5.5kg lysines, 1.4kg methionine, 2.5kg threonines,
11kg calcium dihydrogen phosphates, 11kg stone flours, 4kg salt, the more mineral matters of 1.5kg compound vitamins and 2kg mould inhibitors are (containing 25% the third
Acid) it is mixed with to form auxiliary material.The content of the wherein composition of the more mineral matters of compound vitamin is same as Example 1.
0.2kg bacillus subtilis PB6,3kg egg powder are added into above-mentioned auxiliary material, and (Escherichia coli are specific in egg powder
The potency of Yolk antibody is 6000IU), 0.2kg plants essential oils, 5kg organic acidulants, 1.5kg complex enzyme formulations and 14.4kg ferment
Female extract, it is uniformly mixed into the nursery-stage pig feed of antibiotic-free.Contain cinnamic acid and thymol, Chinese cassia tree wherein in plants essential oil
The mass ratio of aldehyde and thymol is 2:1.Organic acidulant includes lactic acid, citric acid and phosphoric acid, the matter of lactic acid, citric acid and phosphoric acid
Amount is than being 3:3:4.Contain phytase, protease, alpha-amylase and 'beta '-mannase, the child care pig of configuration in complex enzyme formulation
Phytase in feed, protease, the enzyme activity of alpha-amylase and 'beta '-mannase be respectively 2000IU/kg, 6000IU/kg,
1000IU/kg、160000IU/kg。
Embodiment 3
Auxiliary material：By 426.7kg corns, 100kg popcorns, 50kg flour, 30kg soybean oils, 200kg dregs of beans (quality
Fraction be 46%), 50kg fermented bean dregs, 30kg inlet steams fish meal, 25kg whey powders, 25kg glucose, 4kg lysines,
1.4kg methionine, 2.5kg threonines, 10kg calcium dihydrogen phosphates, 10kg stone flours, 4kg salt, the more mineral matters of 1.5kg compound vitamins
And 2kg mould inhibitors (containing 25% propionic acid) are mixed with to form auxiliary material.The wherein component content and reality of the more mineral matters of compound vitamin
It is identical to apply example 1.
0.4kg bacillus subtilis PB6,1kg egg powder (Escherichia coli special yolk in egg powder is added into auxiliary material
The potency of antibody is 8000IU), 0.5kg plants essential oils, 10kg organic acidulants, 1kg complex enzyme formulations and 15kg yeast extraction
Thing, it is uniformly mixed into the nursery-stage pig feed of antibiotic-free.Contain cinnamic acid and thymol, cinnamic acid and hundred wherein in plants essential oil
In phenol mass ratio be 2:1.Organic acidulant includes lactic acid, citric acid and phosphoric acid, and the mass ratio of lactic acid, citric acid and phosphoric acid is
3:3:4.Contain phytase, protease, alpha-amylase and 'beta '-mannase in complex enzyme formulation, in the nursery-stage pig feed of configuration
Phytase, protease, the enzyme activity of alpha-amylase and 'beta '-mannase be respectively 1500IU/kg, 7000IU/kg, 800IU/kg,
240000IU/kg。
Embodiment 4
Auxiliary material：By 459.1kg corns, 50kg popcorns, 30kg flour, 50kg soybean oils, 200kg dregs of beans (quality
Fraction be 46%), 75kg fermented bean dregs, 30kg inlet steams fish meal, 25kg whey powders, 25kg glucose, 4kg lysines,
1.5kg methionine, 3kg threonines, 10kg calcium dihydrogen phosphates, 10kg stone flours, 4kg salt, the more mineral matters of 1.5kg compound vitamins with
And 2kg mould inhibitors (containing 25% propionic acid) are mixed with to form auxiliary material.The wherein composition of the more mineral matters of compound vitamin and the phase of embodiment 1
Together.
0.2kg bacillus subtilis PB6,3kg egg powder (Escherichia coli special yolk in egg powder is added into auxiliary material
The potency of antibody is 5000IU), 0.2kg plants essential oils, 5kg organic acidulants, 1.5kg complex enzyme formulations and 10kg yeast extraction
Thing, it is uniformly mixed into the nursery-stage pig feed of antibiotic-free.Contain cinnamic acid and thymol, cinnamic acid and hundred wherein in plants essential oil
In phenol mass ratio be 1:1.Organic acidulant includes lactic acid, citric acid and phosphoric acid, and the mass ratio of lactic acid, citric acid and phosphoric acid is
3:4:6.Contain phytase, protease, alpha-amylase and 'beta '-mannase in complex enzyme formulation, in the nursery-stage pig feed of configuration
Phytase, protease, the enzyme activity of alpha-amylase and 'beta '-mannase are respectively 2000IU/kg, 8000IU/kg, 1000IU/
kg、320000IU/kg。。
Comparative example 1
Auxiliary material：It is same as Example 1.
It is (anti-that the kitasamycin that olaquindox, 0.1kg mass fractions that 2kg mass fractions are 5% are 50% is added into auxiliary material
Raw element), it is uniformly mixed into the nursery-stage pig feed containing antibiotic.
Comparative example 2
Auxiliary material：It is same as Example 2.
It is (anti-that the kitasamycin that olaquindox, 0.1kg mass fractions that 2kg mass fractions are 5% are 50% is added into auxiliary material
Raw element), it is uniformly mixed into the nursery-stage pig feed containing antibiotic.
Comparative example 3
Auxiliary material：It is same as Example 3.
It is (anti-that the kitasamycin that olaquindox, 0.1kg mass fractions that 2kg mass fractions are 5% are 50% is added into auxiliary material
Raw element), it is uniformly mixed into the nursery-stage pig feed containing antibiotic.
Comparative example 4
Auxiliary material：It is same as Example 4.
It is (anti-that the kitasamycin that olaquindox, 0.1kg mass fractions that 2kg mass fractions are 5% are 50% is added into auxiliary material
Raw element), it is uniformly mixed into the nursery-stage pig feed containing antibiotic.
Comparative example 5
Bacillus subtilis PB6 is not added in feed, remaining composition is same as Example 3.
Comparative example 6
Egg powder is not added in feed, remaining composition is same as Example 3.
Comparative example 7
Plants essential oil is not added in feed, remaining composition is same as Example 3.
Comparative example 8
Organic acidulant is not added in feed, remaining composition is same as Example 3.
Comparative example 9
Complex enzyme formulation is not added in feed, remaining composition is same as Example 3.
Comparative example 10
Yeast extract is not added in feed, remaining composition is same as Example 3.
Test case one
1st, experimental design
Experiment selection is same to write instructions and transfer group, the U.S. system of health (× big × Du of length) the three way cross piglet of body weight in 10.8kg or so
120, it is divided into 2 processing by body weight, feeding respectively often handles 6 repetitions, each using the feed of embodiment 1 and comparative example 1
Repetition has 10 piglets, carries out the feeding experiment of 15 days by a definite date.This is tested exists on November 22,8 days to 2016 November in 2016
The forever auspicious large-scale pig farm for cultivating 1500 livestock on hand sows of Co., Ltd in Guangdong Kaiping City is carried out.
2nd, feeding management
The management of test pig is based on testing the actual feeding management in pig farm, personal management, and each group is raised in identical and managed
Carried out under reason and environmental condition, keep feed fresh, the daily feeding of observation experiment pig, health and active situation.It was found that after sick pig
For the pig number of marking, track record date of the onset, duration, therapeutic scheme, rehabilitation situation, elimination test is wanted in serious morbidity
Column, and the body weight of sick pig is weighed, carry out the record of date and body weight.In order to verify the disease-resistant former effect of patent of the present invention, experiment
Period column home is not cleaned, not rinsed.
3rd, the measure of test index
In on-test, morning empty stomach individual is weighed with the end of, and feed consumption rate is recorded in units of weight, calculates average day
Weightening, average daily gain and feed efficiency.Record child care diarrhea of pigs index daily：It was found that make after diarrhoea sick pig for the pig
Mark number, track record date of the onset, duration, therapeutic scheme, rehabilitation situation；Knock-out experiment column is wanted in serious morbidity, and is claimed
The body weight of sick pig is taken, carries out the record of date and body weight.Normal 0 point of excrement, laxativeness 1 divides, and severe diarrhea 2 divides, according to commenting
Result is divided to calculate every nest Scours index, its calculation formula is：(total day is observed per nest Scours index=observing and recording fraction summation/
Number × per litter size).
Average daily gain (ADG)=(end weight-first starting weight)/(experiment number of days × experiment pig's head number)；
Average daily gain (ADFI)=experiment total feed consumption amount/(experiment number of days × experiment pig's head number)；
Feed efficiency (F/G)=average daily gain/average daily gain.
Per nest Scours index=observe and record fraction summation/(observing total number of days × every litter size)；
The death rate (%)=dead head number/sum × 100.
Result of the test is as shown in table 1 below.
Table 1：The feed of embodiment 1 and the feeding experiment result of comparative example 1
Result of the test shows：The child care pig daily ingestion amount of the feed of embodiment 1 is used to reach for 720g/ heads/day, weightening
438g/ heads/day, feed efficiency (feedstuff-meat ratio) be 1.64, feed intake is more preferable, the speed of growth faster, with containing antibiotic
Comparative example 1 is suitable compared to growth-promoting effect.It is (unclear during experiment to sweep and rush under the feeding environment for the rather harsh deliberately built
Wash column home), Scours index is suitable with there is the feed of antibiotic, and washes in a pan the generation of child care pig without dead, fully achieves containing for comparative example 1
The feeding effect of the nursery-stage pig feed of antibiotic.
Test case two
1st, experimental design
Experiment selection is same to write instructions and transfer group, the U.S. system of health (× big × Du of length) the three way cross piglet 120 of body weight in 10kg or so
Head, it is divided into 2 processing by body weight, feeding respectively often handles 6 repetitions, Mei Gechong using the feed of embodiment 2 and comparative example 2
There are 10 piglets again, carry out the feeding experiment of 15 days by a definite date.This is tested on November 22,8 days to 2016 November in 2016 wide
The forever auspicious large-scale pig farm for cultivating 1500 livestock on hand sows of Co., Ltd in eastern Kaiping City is carried out.
2nd, feeding management
It is identical with test one
3rd, the measure of test index
It is identical with test one
Result of the test is as shown in table 2 below.
Table 2：The feed of embodiment 2 and the feeding experiment result of comparative example 2
Result of the test shows：The child care pig daily ingestion amount of the feed of embodiment 2 is used to reach for 695g/ heads/day, weightening
422g/ heads/day, feed efficiency (feedstuff-meat ratio) are 1.65, and growth is rapid.In the feeding environment for the rather harsh deliberately built
Under (unclear during experiment to sweep and rinse column home), Scours index is suitable with there is the feed of antibiotic, and washes in a pan child care pig without dead and occur,
Fully achieve the feeding effect of the nursery-stage pig feed containing antibiotic of comparative example 2.
Test case three
1st, experimental design
Experiment selection is same to write instructions and transfer group, the U.S. system of health (× big × Du of length) the three way cross piglet of body weight in 10.5kg or so
120, it is divided into 2 processing by body weight, feeding respectively often handles 6 repetitions, each using the feed of embodiment 3 and comparative example 3
Repetition has 10 piglets, carries out the feeding experiment of 15 days by a definite date.This is tested exists on November 22,8 days to 2016 November in 2016
The large-scale pig farm of 1000 livestock on hand sows of Guangdong Huizhou native place lake herding breeding Co., Ltd is carried out.
2nd, feeding management
It is identical with test one
3rd, the measure of test index
It is identical with test one
Result of the test is as shown in table 3 below.
Table 3：The feed of embodiment 3 and the feeding experiment result of comparative example 3
Result of the test shows：The child care pig daily ingestion amount of the feed of embodiment 3 is used to reach for 688g/ heads/day, weightening
426g/ heads/day, feed efficiency (feedstuff-meat ratio) are 1.61, and growth is rapider, and feed efficiency is more preferably.Deliberately seeking
(unclear during experiment to sweep and rinse column home), Scours index and the feed phase for having antibiotic under the feeding environment for the rather harsh made
When, and without the dead feeding effect washed in a pan the generation of child care pig, fully achieve the nursery-stage pig feed containing antibiotic of comparative example 3.
Test case four
1st, experimental design
Experiment selection is same to write instructions and transfer group, the U.S. system of health (× big × Du of length) the three way cross piglet 120 of body weight in 10kg or so
Head, it is divided into 2 processing by body weight, feeding respectively often handles 6 repetitions, Mei Gechong using the feed of embodiment 4 and comparative example 4
There are 10 piglets again, carry out the feeding experiment of 15 days by a definite date.This is tested on November 22,8 days to 2016 November in 2016 wide
The large-scale pig farm of 1000 livestock on hand sows of eastern Huizhou native place lake herding breeding Co., Ltd is carried out.
2nd, feeding management
It is identical with test one
3rd, the measure of test index
It is identical with test one
Result of the test is as shown in table 4 below.
Table 4：The feed of embodiment 4 and the feeding experiment result of comparative example 4
Result of the test shows：The child care pig daily ingestion amount of the feed of embodiment 4 is used to reach for 720g/ heads/day, weightening
447g/ heads/day, feed efficiency (feedstuff-meat ratio) are 1.61, and food calling and gaining effect ratio contain antibiotic group more preferably.Deliberately seeking
(unclear during experiment to sweep and rinse column home), Scours index and the feed phase for having antibiotic under the feeding environment for the rather harsh made
When, and without the dead feeding effect washed in a pan the generation of child care pig, fully achieve the nursery-stage pig feed containing antibiotic of comparative example 4.
Test case five
1st, experimental design
Experiment selection is same to write instructions and transfer group, the U.S. system of health (× big × Du of length) the three way cross piglet of body weight in 10.5kg or so
210, it is divided into 7 processing by body weight, feeding respectively uses the feed of embodiment 3 and 5~comparative example of comparative example 10, every each processing
3 repetitions, it is each to repeat have 10 piglets, carry out the feeding experiment of 15 days by a definite date.This was tested 8 days to 2016 November in 2016
On November 22, in is carried out in the large-scale pig farm of 2000 livestock on hand sows of Guangdong Huizhou Dong Jin agriculture and animal husbandries limited company.
2nd, feeding management
It is identical with test one
3rd, the measure of test index
It is identical with test one
Result of the test is as shown in table 5 below.And with the Comparative result of embodiment 3.
Table 5：The feed of embodiment 3 and the feeding experiment result of 5~comparative example of comparative example 10
In test process, comparative example 5, comparative example 6, comparative example 7 and comparative example 9 have 2,2,3,2 pigs respectively
It is dead.It is intended in the parameter such as child care pig, average daily gain, feed efficiency, Scours index of the feed of feeding embodiment 3 bright
The aobvious feed better than 5~comparative example of raising comparative example 10.It is this explanation probiotics, egg powder, plants essential oil, organic acidulant, multiple
Synthase preparation and each component of yeast extract are cooperated as an entirety, jointly to the health and body of child care pig
Restart improvement.
Each technical characteristic of embodiment described above can be combined arbitrarily, to make description succinct, not to above-mentioned reality
Apply all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, the scope that this specification is recorded all is considered to be.
Embodiment described above only expresses the several embodiments of the present invention, and its description is more specific and detailed, but simultaneously
Can not therefore it be construed as limiting the scope of the patent.It should be pointed out that come for one of ordinary skill in the art
Say, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection of the present invention
Scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (10)
1. a kind of feed addictive, it is characterised in that according to the mass fraction, the feed addictive includes：
The bacterial strain of C.perfringens can be suppressed or kill by containing in the probiotics, contain Escherichia coli in the egg powder
Special yolk antibody, the potency of Escherichia coli special yolk antibody described in the egg powder are 5000IU~8000IU.
2. feed addictive according to claim 1, it is characterised in that the active component in the probiotics is withered grass bud
Spore bacillus PB6.
3. feed addictive according to claim 1, it is characterised in that the plants essential oil is selected from cinnamic acid and thymol
At least one of.
4. feed addictive according to claim 1, it is characterised in that the organic acidulant includes lactic acid, citric acid
And phosphoric acid, the mass ratio of the lactic acid, the citric acid and the phosphoric acid is 1~3:2~4:4~6.
5. feed addictive according to claim 1, it is characterised in that the complex enzyme formulation includes phytase, albumen
Enzyme, alpha-amylase and 'beta '-mannase.
A kind of 6. antibiotic-free feed, it is characterised in that including the feed addictive as described in any one of Claims 1 to 5 and
Acceptable auxiliary material in feed science.
7. antibiotic-free feed according to claim 6, it is characterised in that the feed addictive accounts for the antibiotic-free
The 0.5%~5% of quality of the fodder percentage composition.
8. antibiotic-free feed according to claim 6, it is characterised in that according to the mass fraction, the auxiliary material includes：
80 parts~99 parts of energy and protein material；
1 part~5 parts of premix；And
0.1 part~0.5 part of mould inhibitor；
Wherein, the energy and protein material are used to provide basic energy and protein requirement, and the premix is used to carry
For trace element and supplement the nutrients, the mould inhibitor is used to prevent the feed from going mouldy.
9. antibiotic-free feed according to claim 8, it is characterised in that the energy and protein material are according to quality
Number meter includes 30 parts~60 parts of corn, 0.01 part~15 parts of popcorn, 5 parts~10 parts of flour, 2 parts~5 parts big
Soya-bean oil, 16 parts~22 parts of dregs of beans, 2.5 parts~7.5 parts of fermented bean dregs, 2 parts~4 parts fish meal, 0.01 part~5 portions whey powders and
2.5 parts~5 parts glucose；And/or
The premix include 0.3 part~0.9 part amino acid, 1 part~1.4 parts of calcium dihydrogen phosphate, 0.50 part~1.00 parts
Stone flour, 0.3 part~0.5 part of sodium chloride and 0.1 part~0.2 part of the more mineral matters of compound vitamin.
10. feed addictive as described in any one of Claims 1 to 5 or as described in any one of claim 6~9 without antibiosis
Application of the plain feed in nursery-stage pig feed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710775139.7A CN107484879A (en) | 2017-08-31 | 2017-08-31 | Feed addictive, antibiotic-free feed and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710775139.7A CN107484879A (en) | 2017-08-31 | 2017-08-31 | Feed addictive, antibiotic-free feed and its application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107484879A true CN107484879A (en) | 2017-12-19 |
Family
ID=60650937
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710775139.7A Pending CN107484879A (en) | 2017-08-31 | 2017-08-31 | Feed addictive, antibiotic-free feed and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107484879A (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108432946A (en) * | 2018-06-20 | 2018-08-24 | 江苏强森生物科技有限公司 | A kind of feed addictive and preparation method thereof of prevention animal diarrhea |
| CN108813165A (en) * | 2018-05-29 | 2018-11-16 | 福建农业职业技术学院 | Pig Lavender feed addictive and preparation method thereof |
| CN109170273A (en) * | 2018-10-16 | 2019-01-11 | 广西康佳龙农牧集团有限公司 | A kind of new probiotic bacterium nonreactive particulate material and preparation method thereof |
| CN109730200A (en) * | 2019-02-17 | 2019-05-10 | 长沙微威生物科技有限公司 | A kind of combination enzyme preparation solving beta-mannase activation innate immune reaction |
| CN109864180A (en) * | 2019-04-01 | 2019-06-11 | 河南旭百瑞生物科技股份有限公司 | A kind of novel nonreactive powder adds grain shape groove feed for pigling and preparation method thereof |
| CN110771729A (en) * | 2019-11-01 | 2020-02-11 | 广西壮族自治区林业科学研究院 | Feed additive containing plant essential oil and preparation method thereof |
| CN111227116A (en) * | 2019-11-22 | 2020-06-05 | 武汉新华扬生物股份有限公司 | Black-bone chicken feed additive and preparation method thereof |
| CN111758835A (en) * | 2020-06-11 | 2020-10-13 | 广西壮族自治区动物疫病预防控制中心 | Feed for young bamboo rats and preparation method thereof |
| CN112931698A (en) * | 2019-11-26 | 2021-06-11 | 浏阳乐佰有机营养有限公司 | Feed additive and antibiotic-free low-zinc feed |
| WO2025050396A1 (en) * | 2023-09-08 | 2025-03-13 | Elanco (Shanghai) Animal Health Co., Ltd | Probiotic animal feed compositions |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1981036A (en) * | 2003-12-19 | 2007-06-13 | 辛根塔参与股份公司 | Microbially expressed xylanases and their use as feed additives and other uses |
| CN105211542A (en) * | 2015-10-14 | 2016-01-06 | 山东丰沃新农农牧科技有限公司 | A kind of piglet preweaning feed and preparation method thereof |
| CN105394392A (en) * | 2015-11-24 | 2016-03-16 | 天津市中升挑战生物科技有限公司 | Feed additive capable of resisting swine viral diseases and preparation method for feed additive |
| CN105901305A (en) * | 2014-05-19 | 2016-08-31 | 张兴龙 | Antibiotic substitute feed additive, its formula, feed with antibiotic substitute feed additive and production method of feed with antibiotic substitute feed additive |
| CN106689738A (en) * | 2016-11-30 | 2017-05-24 | 上海新邦生物科技有限公司 | Sow and piglet integral nutrition scheme |
| CN106804938A (en) * | 2017-01-17 | 2017-06-09 | 深圳市金新农科技股份有限公司 | Fish meal substitute composition, compound premix and without fish meal nursery-stage pig feed |
| CN106804919A (en) * | 2015-11-30 | 2017-06-09 | 青岛顺昕电子科技有限公司 | A kind of sucking pig proprietary formula milk powder |
| CN106822890A (en) * | 2017-01-19 | 2017-06-13 | 中国农业科学院兰州畜牧与兽药研究所 | A kind of pharmaceutical composition for preventing and treating diarrhea of weaned piglets and its preparation method and application |
| CN106852397A (en) * | 2015-12-08 | 2017-06-16 | 北京科高大北农饲料有限责任公司 | A kind of sow feed and preparation method thereof |
-
2017
- 2017-08-31 CN CN201710775139.7A patent/CN107484879A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1981036A (en) * | 2003-12-19 | 2007-06-13 | 辛根塔参与股份公司 | Microbially expressed xylanases and their use as feed additives and other uses |
| CN105901305A (en) * | 2014-05-19 | 2016-08-31 | 张兴龙 | Antibiotic substitute feed additive, its formula, feed with antibiotic substitute feed additive and production method of feed with antibiotic substitute feed additive |
| CN105211542A (en) * | 2015-10-14 | 2016-01-06 | 山东丰沃新农农牧科技有限公司 | A kind of piglet preweaning feed and preparation method thereof |
| CN105394392A (en) * | 2015-11-24 | 2016-03-16 | 天津市中升挑战生物科技有限公司 | Feed additive capable of resisting swine viral diseases and preparation method for feed additive |
| CN106804919A (en) * | 2015-11-30 | 2017-06-09 | 青岛顺昕电子科技有限公司 | A kind of sucking pig proprietary formula milk powder |
| CN106852397A (en) * | 2015-12-08 | 2017-06-16 | 北京科高大北农饲料有限责任公司 | A kind of sow feed and preparation method thereof |
| CN106689738A (en) * | 2016-11-30 | 2017-05-24 | 上海新邦生物科技有限公司 | Sow and piglet integral nutrition scheme |
| CN106804938A (en) * | 2017-01-17 | 2017-06-09 | 深圳市金新农科技股份有限公司 | Fish meal substitute composition, compound premix and without fish meal nursery-stage pig feed |
| CN106822890A (en) * | 2017-01-19 | 2017-06-13 | 中国农业科学院兰州畜牧与兽药研究所 | A kind of pharmaceutical composition for preventing and treating diarrhea of weaned piglets and its preparation method and application |
Non-Patent Citations (2)
| Title |
|---|
| 姜锡瑞，霍兴云，黄继红，孙中涛: "《生物发酵酶产业技术》", 31 December 2016 * |
| 龚新威 李勇: "《饲料添加剂实用手册》", 31 December 1999 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108813165A (en) * | 2018-05-29 | 2018-11-16 | 福建农业职业技术学院 | Pig Lavender feed addictive and preparation method thereof |
| CN108432946A (en) * | 2018-06-20 | 2018-08-24 | 江苏强森生物科技有限公司 | A kind of feed addictive and preparation method thereof of prevention animal diarrhea |
| CN109170273A (en) * | 2018-10-16 | 2019-01-11 | 广西康佳龙农牧集团有限公司 | A kind of new probiotic bacterium nonreactive particulate material and preparation method thereof |
| CN109730200A (en) * | 2019-02-17 | 2019-05-10 | 长沙微威生物科技有限公司 | A kind of combination enzyme preparation solving beta-mannase activation innate immune reaction |
| CN109864180A (en) * | 2019-04-01 | 2019-06-11 | 河南旭百瑞生物科技股份有限公司 | A kind of novel nonreactive powder adds grain shape groove feed for pigling and preparation method thereof |
| CN110771729A (en) * | 2019-11-01 | 2020-02-11 | 广西壮族自治区林业科学研究院 | Feed additive containing plant essential oil and preparation method thereof |
| CN111227116A (en) * | 2019-11-22 | 2020-06-05 | 武汉新华扬生物股份有限公司 | Black-bone chicken feed additive and preparation method thereof |
| CN112931698A (en) * | 2019-11-26 | 2021-06-11 | 浏阳乐佰有机营养有限公司 | Feed additive and antibiotic-free low-zinc feed |
| CN111758835A (en) * | 2020-06-11 | 2020-10-13 | 广西壮族自治区动物疫病预防控制中心 | Feed for young bamboo rats and preparation method thereof |
| WO2025050396A1 (en) * | 2023-09-08 | 2025-03-13 | Elanco (Shanghai) Animal Health Co., Ltd | Probiotic animal feed compositions |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107484879A (en) | Feed addictive, antibiotic-free feed and its application | |
| CN110573606B (en) | Bacillus subtilis strain for improving animal performance parameters | |
| Cromwell | Antimicrobial and promicrobial agents | |
| US11607434B2 (en) | Bacillus compositions and methods of use with ruminants | |
| US10834942B2 (en) | Yeast and bacterial probiotics combinations and methods of use to improve swine production | |
| CN103734550B (en) | A kind of nursery pig concentrated feed | |
| JP2021141899A (en) | Feed additive composition | |
| US8021654B2 (en) | Methods of treating pigs with Bacillus strains | |
| CN105475624B (en) | Microbial fermentation feed and production method and application thereof | |
| CN102894218B (en) | Milk replacer of 0-3 month lambs and production method thereof | |
| CN103766661B (en) | A kind of not containing the piglet preweaning feed of pig homology raw material | |
| CN103689273A (en) | Non-antibiotics compound feed for suckling pig | |
| JP2023510910A (en) | Compositions and methods for controlling unwanted microorganisms and improving animal health | |
| El-Badawi | Growth performance of male NZW rabbits fed diets supplemented with beneficial bacteria or live yeast | |
| Matusevičius et al. | Effect of probiotic bioplus 2B® on performance of growing rabbit | |
| CN105325729A (en) | Compound pig feed additive and preparation method thereof | |
| CN106578354A (en) | Piglet compound premix, preparation method and daily ration for piglet | |
| JP2021516258A (en) | Use of direct feeding microorganisms in the prevention and / or treatment of E. coli-based infections in animals | |
| KR20090078065A (en) | Antimicrobial-enhanced Lactic Acid Bacteria Preparation and its Manufacturing Method | |
| Pasupathy et al. | Effect of lactobacillus supplementation on growth and nutrient utilization in mongrel pups | |
| CN102754733A (en) | Novel micro-ecologic preparation for pig and preparation method thereof | |
| CN105876190A (en) | Lactobacillus-containing nursery feed for weaned piglets and preparation method thereof | |
| Tag El Din | Effect of dry live yeast inclusion in fattening rabbit diets on productive performance and digestibility of nutrients | |
| CN107624979A (en) | A kind of mixed feed piglet cradling early stage | |
| CN109122551B (en) | Pig raising method for preventing pig coccidiosis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171219 |