CN102433215A - Method for extracting grease from fungi or algae by physical wall breaking - Google Patents
Method for extracting grease from fungi or algae by physical wall breaking Download PDFInfo
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- CN102433215A CN102433215A CN2011102835257A CN201110283525A CN102433215A CN 102433215 A CN102433215 A CN 102433215A CN 2011102835257 A CN2011102835257 A CN 2011102835257A CN 201110283525 A CN201110283525 A CN 201110283525A CN 102433215 A CN102433215 A CN 102433215A
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- 241000195493 Cryptophyta Species 0.000 title claims abstract description 69
- 241000233866 Fungi Species 0.000 title claims abstract description 42
- 239000004519 grease Substances 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 28
- 239000007788 liquid Substances 0.000 claims abstract description 45
- 238000000926 separation method Methods 0.000 claims abstract description 19
- 238000000855 fermentation Methods 0.000 claims abstract description 15
- 230000004151 fermentation Effects 0.000 claims abstract description 15
- 239000000839 emulsion Substances 0.000 claims abstract description 14
- 238000003756 stirring Methods 0.000 claims description 48
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 46
- 238000009826 distribution Methods 0.000 claims description 22
- 239000000203 mixture Substances 0.000 claims description 21
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 20
- 239000000284 extract Substances 0.000 claims description 15
- 230000015556 catabolic process Effects 0.000 claims description 13
- 239000002253 acid Substances 0.000 claims description 11
- 229930003427 Vitamin E Natural products 0.000 claims description 10
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 10
- 235000019165 vitamin E Nutrition 0.000 claims description 10
- 229940046009 vitamin E Drugs 0.000 claims description 10
- 239000011709 vitamin E Substances 0.000 claims description 10
- 241001530490 Salvia rosmarinus Species 0.000 claims description 9
- 239000003513 alkali Substances 0.000 claims description 9
- 235000015639 rosmarinus officinalis Nutrition 0.000 claims description 9
- 241000199914 Dinophyceae Species 0.000 claims description 8
- IPCSVZSSVZVIGE-UHFFFAOYSA-M hexadecanoate Chemical compound CCCCCCCCCCCCCCCC([O-])=O IPCSVZSSVZVIGE-UHFFFAOYSA-M 0.000 claims description 8
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 6
- 239000003112 inhibitor Substances 0.000 claims description 6
- 241000024188 Andala Species 0.000 claims description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 4
- 241000907999 Mortierella alpina Species 0.000 claims description 4
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 4
- 241000306282 Umbelopsis isabellina Species 0.000 claims description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 4
- 238000013019 agitation Methods 0.000 claims description 2
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 2
- 239000004310 lactic acid Substances 0.000 claims description 2
- 235000014655 lactic acid Nutrition 0.000 claims description 2
- 239000004576 sand Substances 0.000 claims description 2
- 238000010792 warming Methods 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 abstract description 92
- 239000003963 antioxidant agent Substances 0.000 abstract description 9
- 230000003078 antioxidant effect Effects 0.000 abstract description 9
- 210000002421 cell wall Anatomy 0.000 abstract description 7
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 abstract description 7
- 230000008569 process Effects 0.000 abstract description 5
- 231100000252 nontoxic Toxicity 0.000 abstract description 2
- 230000003000 nontoxic effect Effects 0.000 abstract description 2
- 239000004480 active ingredient Substances 0.000 abstract 1
- 238000000498 ball milling Methods 0.000 abstract 1
- 239000000084 colloidal system Substances 0.000 abstract 1
- 238000009776 industrial production Methods 0.000 abstract 1
- 238000003860 storage Methods 0.000 description 32
- 238000000605 extraction Methods 0.000 description 13
- 238000001816 cooling Methods 0.000 description 11
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 11
- YZXBAPSDXZZRGB-DOFZRALJSA-N arachidonic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O YZXBAPSDXZZRGB-DOFZRALJSA-N 0.000 description 10
- 239000002826 coolant Substances 0.000 description 10
- 239000000498 cooling water Substances 0.000 description 10
- 230000001105 regulatory effect Effects 0.000 description 10
- 150000003839 salts Chemical class 0.000 description 10
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 10
- DVSZKTAMJJTWFG-UHFFFAOYSA-N docosa-2,4,6,8,10,12-hexaenoic acid Chemical compound CCCCCCCCCC=CC=CC=CC=CC=CC=CC(O)=O DVSZKTAMJJTWFG-UHFFFAOYSA-N 0.000 description 9
- MBMBGCFOFBJSGT-KUBAVDMBSA-N docosahexaenoic acid Natural products CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCC(O)=O MBMBGCFOFBJSGT-KUBAVDMBSA-N 0.000 description 9
- 235000006708 antioxidants Nutrition 0.000 description 8
- 244000055346 Paulownia Species 0.000 description 7
- 230000002538 fungal effect Effects 0.000 description 6
- 229940114079 arachidonic acid Drugs 0.000 description 5
- 235000021342 arachidonic acid Nutrition 0.000 description 5
- 235000020673 eicosapentaenoic acid Nutrition 0.000 description 5
- 235000021323 fish oil Nutrition 0.000 description 5
- 239000003960 organic solvent Substances 0.000 description 5
- 238000009775 high-speed stirring Methods 0.000 description 4
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 230000004888 barrier function Effects 0.000 description 2
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- 238000006243 chemical reaction Methods 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 235000020660 omega-3 fatty acid Nutrition 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- DPUOLQHDNGRHBS-UHFFFAOYSA-N Brassidinsaeure Natural products CCCCCCCCC=CCCCCCCCCCCCC(O)=O DPUOLQHDNGRHBS-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical group [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 206010008132 Cerebral thrombosis Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- URXZXNYJPAJJOQ-UHFFFAOYSA-N Erucic acid Natural products CCCCCCC=CCCCCCCCCCCCC(O)=O URXZXNYJPAJJOQ-UHFFFAOYSA-N 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 201000001429 Intracranial Thrombosis Diseases 0.000 description 1
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 1
- 102000004895 Lipoproteins Human genes 0.000 description 1
- 108090001030 Lipoproteins Proteins 0.000 description 1
- HXWJFEZDFPRLBG-UHFFFAOYSA-N Timnodonic acid Natural products CCCC=CC=CCC=CCC=CCC=CCCCC(O)=O HXWJFEZDFPRLBG-UHFFFAOYSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- JAZBEHYOTPTENJ-JLNKQSITSA-N all-cis-5,8,11,14,17-icosapentaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O JAZBEHYOTPTENJ-JLNKQSITSA-N 0.000 description 1
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 description 1
- 235000020661 alpha-linolenic acid Nutrition 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000003925 brain function Effects 0.000 description 1
- 230000015015 brainstem development Effects 0.000 description 1
- 150000001721 carbon Chemical group 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000003130 cardiopathic effect Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000005008 domestic process Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 229960005135 eicosapentaenoic acid Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- DPUOLQHDNGRHBS-KTKRTIGZSA-N erucic acid Chemical compound CCCCCCCC\C=C/CCCCCCCCCCCC(O)=O DPUOLQHDNGRHBS-KTKRTIGZSA-N 0.000 description 1
- 238000004880 explosion Methods 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 210000003754 fetus Anatomy 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 229960004232 linoleic acid Drugs 0.000 description 1
- 229960004488 linolenic acid Drugs 0.000 description 1
- KQQKGWQCNNTQJW-UHFFFAOYSA-N linolenic acid Natural products CC=CCCC=CCC=CCCCCCCCC(O)=O KQQKGWQCNNTQJW-UHFFFAOYSA-N 0.000 description 1
- 208000010125 myocardial infarction Diseases 0.000 description 1
- 230000008271 nervous system development Effects 0.000 description 1
- 230000007514 neuronal growth Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 229940127293 prostanoid Drugs 0.000 description 1
- 150000003814 prostanoids Chemical class 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 125000005480 straight-chain fatty acid group Chemical group 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/04—Pretreatment of vegetable raw material
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/06—Production of fats or fatty oils from raw materials by pressing
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/16—Refining fats or fatty oils by mechanical means
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Mechanical Engineering (AREA)
- Microbiology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Edible Oils And Fats (AREA)
Abstract
The invention discloses a method for extracting grease from fungi or algae cells rich in grease by physical wall breaking, which mainly separates fermented fungi or algae fermentation liquor by a separation system and collects cell control cell mud, physically breaks the wall of the cell mud by a colloid mill or a high-pressure homogenizer, performs secondary ball milling wall breaking and emulsion breaking by a ball mill, and finally separates feed liquid by a three-phase separator to obtain the grease rich in polyunsaturated fatty acid. The invention adopts physical wall breaking and extracting methods, has simple process and high cell wall breaking efficiency, can effectively protect active ingredients in algae and fungi cells due to low temperature and antioxidant treatment, has green, non-toxic and residue-free products, can be used for extracting DHA grease, ARA grease, EPA grease, GLA grease, ALA grease and the like, and is suitable for industrial production.
Description
Technical field
The present invention relates to a kind of from fungi or algae the method for extracting effective components, particularly relate to a kind of from fungi or algae physical wall breaking extract greasy method.
Background technology
Pufas (polyunsaturated fatty acid; PUFA) generally be meant and contain two or more pairs key; The straight chain fatty acid of carbon chain lengths 16-22; Position difference according to two keys place carbon atom is divided into serial unsaturated fatty acids of ω-3 and the serial unsaturated fatty acids of ω-6 again, and wherein linolenic acid (GLA), timnodonic acid (EPA), docosahexenoic acid (DHA) belong to ω-3 series, and linolic acid, arachidonic acid (ARA) belong to ω-6 series.It is active that PUFA has many important physical, and it can regulate the homergy of interior blood fat of human body and lipoprotein, reduces cholesterol level in blood viscosity and the blood; The protection vessel wall; Prevent the formation of cerebral thrombosis, have the prevention myocardial infarction, hypertension, cardiopathic function; Thereby it can be converted to the effect that prostanoid plays immunne response in human body simultaneously, and aspects such as enhance immunity are all had positive effect; Some composition in the unsaturated fatty acids still is the indispensable nutritive substance of brain and nervous system development in addition; Can promote the neural growth of fetus brain like the DHA in the pufas; Significantly improve the infant intelligent development level; Simultaneously, DHA delays the brain aging and plays an important role keeping the brain function.
Human body self can not synthesize polyunsaturated fatty acid, but it is again one of indispensable important substance of human body, therefore can only satisfy the human body needs through absorbing from the external world.The main source of traditional unsaturated fatty acids is a fish oil, but fish oil itself has many shortcomings, as: unpleasant fishy smell, mouthfeel be not good, contain SUV and the erucic acid that is bound to arouse fear etc., greatly influences the quality of product; And unsaturated fatty acid content receives influences such as season, area, kind and fish stock restriction easily in the fish oil; In addition, owing to receive the influence of environmental pollution, the fish of certain areas possibly contain heavy metal and other pollutents of high level, and this can extra increase extraction cost.
Discover, contain a large amount of unsaturated fatty acidss in many fungies and the alga cells.From fungi or algae, extract unsaturated fatty acids and can overcome existing the problems referred to above of extraction unsaturated fatty acids from fish oil; And fungi and algae can carry out mass artificial and cultivate; Output is high; Cycle is short, therefore utilizes fungi and algae to extract unsaturated fatty acids as the alternate resources of fish oil and has far reaching significance.
Because fungi and algae self cell walls, the problem of from fungi or alga cells, extracting the maximum of unsaturated fatty acids is a cell wall breaking.The domestic method of from fungi or alga cells, extracting unsaturated fatty acids is: organic solvent method, acid heat method, enzyme process etc.Organic solvent method has advantage simple to operate, but time-consuming, and extraction efficiency is low, has problems such as product residue organic solvent and production safety hidden danger simultaneously.Adopt the acid heat method can destroy the cell walls of fungi or algae fast and effectively, but reaction conditions is violent, destroys desired product composition in the cell easily, and have problems such as sour residual.Adopt enzymatic shell-broken, though reaction conditions is gentle, equipment is simple; But because the difference of cell walls moity between different microorganisms, different microorganisms iuntercellular shell-broken effect significant difference is simultaneously because enzymatic hydrolysis condition is harsh than additive method; Therefore the enzymolysis cost is higher, is not suitable for suitability for industrialized production.
Summary of the invention
The present invention aims to provide a kind of the auxiliary of any organic solvent that need not, and the method for employing physics is to fungi or alga cells carries out broken wall and extracts grease in the born of the same parents, is suitable for scale operation cheaply.
For achieving the above object, technical scheme proposed by the invention is: a kind of from fungi or algae physical wall breaking extract greasy method, it is characterized in that: comprise the steps:
Step 1: fungi after the fermentation ends or algae fermented liquid are passed through the separation system collecting cell, keep fungi or frustule mud water cut at 70-90%;
Step 2: control cell mud temperature is at 15-40 ℃;
Step 3: use acid or alkali to regulate cell mud PH and be 0-5 or 8-14;
Step 4: the inhibitor that in cell mud, adds cell shale amount 1-3%;
Step 5: the cell dredge pump is gone into colloidal mill or high pressure homogenizer carries out broken wall, and controlled temperature is at 30-95 ℃;
Step 6: the cell mud that colloidal mill or high pressure homogenizer come out carries out secondary broken wall and breakdown of emulsion through ball mill, and controlled temperature is at 30-95 ℃;
Step 7: the cell mud behind the broken wall is warming up to 30-95 ℃ under the agitation condition of 20-150rpm, and keeps 0.5-5 hour;
Step 8: the bottom adds hot water through gas distribution pipe by weight cell Ni ﹕ water=1 ﹕ 2.5-6.5 from cell mud, and controlled temperature 30-95 ℃, stir 50-80min at 250-350rpm, feed liquid is separated obtaining grease through three-phase separator.
Further, described in the step 4 inhibitor be vitamin E, natural Rosmarinus officinalis, a kind of in the anti-bad courageous and upright sour cetylate or their mixture.
Further, the WP of the high pressure homogenizer described in the step 6 is 120-130Mpa.
Further, the working speed of colloidal mill is below the 14000rpm in the step 6.
Further, described acid is hydrochloric acid, sulfuric acid, Hydrocerol A or lactic acid.
Further, described alkali is sodium hydroxide.
Further, described ball mill is sand mill or ball mill etc., and its WP is 20-30Mpa.
Further, described algae is for splitting kettle algae or dino flagellate, and grease is the DHA grease.
Further, described fungi is a Mortierella alpina, and grease is the ARA grease.
Further, described fungi is a Mortierella isabellina, and grease is the EPA grease.
Further, described fungi is little Ke Yinhan, and grease is GLA and ALA grease.
The present invention has overcome and must carry out drying traditionally and process powder and just pulverize, and the technological barrier that is difficult to the hard cell walls of broken fungi or algae, has realized low-cost and the cell walls of high efficiency broken algae and fungi.Its concrete characteristic is following: (1) is for avoiding fungi and alga cells mud under high velocity impact or high-pressure squeezing condition; Be easy to generate heat; And the biological activity of destruction intracellular product; The means of the present invention's employing cooling in advance are reduced to 15-40 ℃ with the temperature of fungi and alga cells mud; Adjust simultaneously storage that pH more helps feed liquid controlled effectively fungi and alga cells mud particularly in the born of the same parents polyunsaturated fatty acid grease be exposed in the high temperature, the effective constituent of having avoided Yin Gaowen to cause is decomposed or inactivation, adjusts pH simultaneously and more helps the storage of feed liquid and improve sporoderm-broken rate; (2) added protective material in advance; In high pressure homogenizer or colloidal mill broken wall process; Protective material can be uniformly and effectively with grease behind the broken wall or particle embedding; Form effectively protection barrier, thus reduce pufas in the born of the same parents greatly with the contacting of air, reduce the possibility of the oxidized decomposition of polyunsaturated fatty acid grease.(3) adopt the process for extracting of physics, overcome the residual and hidden peril of explosion that organic solvent traditionally brings, a kind of product of safe green nontoxic residue-free is provided; (4) the cell mud that adopts colloidal mill or high pressure homogenizer to come out carries out secondary broken wall and breakdown of emulsion through ball mill, has improved productive rate.
Embodiment
Below in conjunction with specific embodiment, the present invention is further specified.
Embodiment 1
1) the kettle algae fermented liquid that splits after the fermentation ends is passed through separation system separated and collected frustule, keep algae mud water cut at 70%-90%; Algae mud is collected in the storage tank that has spiral coil cooling tube and a stirring,
2) be that 10 ℃ of water coolants cool off through in the coil pipe of tank body, feeding temperature, wait the temperature of algae mud to reduce to 15-40 ℃, close cooling water intakeoutfall;
3) under stirring state, utilize acid for adjusting pH, make algae mud pH between 0.0-5.0;
4) under stirring state, add 3% antioxidant blends (vitamin E 1%, natural Rosmarinus officinalis 0.5%, anti-bad courageous and upright sour cetylate 1.5%) according to algae mud weight ratio, mix;
5) the algae dredge pump is gone in the paulownia body of high pressure homogenizer, the WP of regulating high pressure homogenizer is 120-130Mpa, and the temperature that keeps high pressure homogenizer is carried out high-pressure homogeneous broken wall at 30-95 ℃ with algae mud, and the feed liquid behind the broken wall is directly squeezed in the storage tank of ball mill;
6) pressure of adjusting ball mill is 30Mpa, and controlled temperature carries out secondary broken wall and breakdown of emulsion at 30-95 ℃, and the feed liquid behind the secondary broken wall is packed into and had in the storage tank of gas distribution pipeline and coil pipe;
7) the kettle algae algae mud that splits behind the broken wall is warmed up to 30-95 ℃, under 20-150rpm stirs, heats and kept 0.5-5 hour;
8) ratio by weight Zao Ni ﹕ water=1 ﹕ 2.5-6.5 adds hot water from algae mud bottom through gas distribution pipe; And control is expected warm 30-95 ℃; Add an amount of edible salt and under 250-350rpm stirs, keep 50-80min, feed liquid is separated obtaining the DHA grease through three-phase separator, extraction yield is 93%.
Embodiment 2
1) dino flagellate fermentation liquor after the fermentation ends is utilized separation system separated and collected dino flagellate cell, keep algae mud water cut at 70%-90%; Algae mud is collected in the storage tank that has spiral coil cooling tube and a stirring;
2) be that 10 ℃ of water coolants cool off through in the coil pipe of tank body, feeding temperature, wait the temperature of algae mud to reduce to 20 ℃, close cooling water intakeoutfall;
3) under the high-speed stirring situation, utilize acid for adjusting pH, make between the algae mud pH 0.0-5.0;
4) under the high-speed stirring situation, add 1% antioxidant blends (vitamin E 0.3%, natural Rosmarinus officinalis 0.5%, anti-bad courageous and upright sour cetylate 0.2%) according to algae mud weight ratio, mix;
5) with the algae dredge pump go into colloidal mill in, the working speed of regulating colloidal mill is below the 14000rpm, controlled temperature carries out broken wall at 30-95 ℃ with algae mud, the feed liquid behind the broken wall is directly squeezed in the storage tank of ball mill;
6) pressure of adjusting ball mill is 20Mpa, and controlled temperature carries out secondary broken wall and breakdown of emulsion at 30-95 ℃, and the feed liquid behind the secondary broken wall is packed into and had in the storage tank of gas distribution pipeline and coil pipe;
7) the dino flagellate algae mud behind the broken wall is warmed up to 30-95 ℃, under 20-150rpm stirs, heats and kept 0.5-5 hour;
8) ratio by weight Zao Ni ﹕ water=1 ﹕ 2.5-6.5 adds hot water from algae mud bottom through gas distribution pipe; And control is expected warm 30-95 ℃; Add an amount of edible salt and under 250-350rpm stirs, keep 50-80min, feed liquid is separated obtaining the DHA grease through three-phase separator, extraction yield is 88%.
Embodiment 3
1) the Mortierella alpina fermented liquid after the fermentation ends is passed through separation system separated and collected fungal cell, keep cell mud water cut at 70%-90%; Cell mud is collected in the storage tank that has spiral coil cooling tube and a stirring,
2) be that 10 ℃ of water coolants cool off through in the coil pipe of tank body, feeding temperature, wait the temperature of cell mud to reduce to 15-40 ℃, close cooling water intakeoutfall;
3) under stirring state, utilize acid for adjusting pH, make between the cell mud pH 0.0-5.0;
4) under stirring state, add 2% antioxidant blends (vitamin E 0.5%, natural Rosmarinus officinalis 0.5%, anti-bad courageous and upright sour cetylate 1%) according to cell mud weight ratio, mix;
5) the cell dredge pump is gone in the paulownia body of high pressure homogenizer; The WP of regulating high pressure homogenizer is 120-130Mpa; The temperature that keeps high pressure homogenizer is carried out high-pressure homogeneous broken wall at 30-95 ℃ with cell mud, and the feed liquid behind the broken wall is directly squeezed in the storage tank of ball mill;
6) pressure of adjusting ball mill is 25Mpa, and controlled temperature carries out secondary broken wall and breakdown of emulsion at 30-95 ℃, and the feed liquid behind the secondary broken wall is packed into and had in the storage tank of gas distribution pipeline and coil pipe;
7) the cell mud behind the broken wall is warmed up to 30-95 ℃, under 20-150rpm stirs, heats and kept 0.5-5 hour;
8) ratio by weight cell Ni ﹕ water=1 ﹕ 2.5-6.5 adds hot water from cell mud bottom through gas distribution pipe; And control is expected warm 30-95 ℃; Add an amount of edible salt and under 250-350rpm stirs, keep 50-80min; Feed liquid is obtained pufas ARA grease through the three-phase separator separation, and extraction yield is 83%.
Embodiment 4
1) the Mortierella isabellina fermented liquid after the fermentation ends is passed through separation system separated and collected fungal cell, keep cell mud water cut at 70%-90%; Cell mud is collected in the storage tank that has spiral coil cooling tube and a stirring,
2) be that 10 ℃ of water coolants cool off through in the coil pipe of tank body, feeding temperature, wait the temperature of cell mud to reduce to 15-40 ℃, close cooling water intakeoutfall;
3) under stirring state, utilize acid to carry out the allotment of pH, make between the cell mud pH 0.0-5.0;
4) under stirring state, add 1% antioxidant vitamin E according to cell mud weight ratio, mix;
5) with the cell dredge pump go into colloidal mill in, the working speed of regulating colloidal mill is below the 14000rpm, controlled temperature carries out high-pressure homogeneous broken wall at 30-95 ℃ with cell mud, the feed liquid behind the broken wall is directly squeezed in the storage tank of ball mill;
6) pressure of adjusting ball mill is 30Mpa, and controlled temperature carries out secondary broken wall and breakdown of emulsion at 30-95 ℃, and the feed liquid behind the secondary broken wall is packed into and had in the storage tank of gas distribution pipeline and coil pipe;
7) the cell mud behind the broken wall is warmed up to 30-95 ℃, under 20-150rpm stirs, heats and kept 0.5-5 hour;
8) ratio by weight cell Ni ﹕ water=1 ﹕ 2.5-6.5 adds hot water from cell mud bottom through gas distribution pipe; And control is expected warm 30-95 ℃; Add an amount of edible salt and under 250-350rpm stirs, keep 50-80min; Feed liquid is obtained pufas EPA grease through the three-phase separator separation, and extraction yield is 85%.
Embodiment 5
1) the little Ke Yinhan fermented liquid after the fermentation ends is passed through separation system separated and collected fungal cell, keep cell mud water cut at 70%-90%; Cell mud is collected in the storage tank that has spiral coil cooling tube and a stirring,
2) be that 10 ℃ of water coolants cool off through in the coil pipe of tank body, feeding temperature, wait the temperature of cell mud to reduce to 15-40 ℃, close cooling water intakeoutfall;
3) under stirring state, utilize acid for adjusting pH, make between the cell mud pH 0.0-5.0;
4) under stirring state, add the natural Rosmarinus officinalis of 2% inhibitor according to cell mud weight ratio, mix;
5) the cell dredge pump is gone in the paulownia body of high pressure homogenizer; The WP of regulating high pressure homogenizer is 120-130Mpa; The temperature that keeps high pressure homogenizer is carried out high-pressure homogeneous broken wall at 30-95 ℃ with cell mud, and the feed liquid behind the broken wall is directly squeezed in the storage tank of ball mill;
6) pressure of adjusting ball mill is 20Mpa, and controlled temperature carries out secondary broken wall and breakdown of emulsion at 30-95 ℃, and the feed liquid behind the secondary broken wall is packed into and had in the storage tank of gas distribution pipeline and coil pipe;
7) the cell mud behind the broken wall is warmed up to 30-95 ℃, under 20-150rpm stirs, heats and kept 0.5-5 hour;
8) ratio by weight cell Ni ﹕ water=1 ﹕ 2.5-6.5 adds hot water from cell mud bottom through gas distribution pipe; And control is expected warm 30-95 ℃; Add an amount of edible salt and under 250-350rpm stirs, keep 50-80min; Feed liquid is obtained pufas GLA and ALA grease through the three-phase separator separation, and extraction yield is 82%.
Embodiment 6
1) the kettle algae fermented liquid that splits after the fermentation ends is passed through separation system separated and collected frustule, keep algae mud water cut at 70%-90%; Algae mud is collected in the storage tank that has spiral coil cooling tube and a stirring,
2) be that 10 ℃ of water coolants cool off through in the coil pipe of tank body, feeding temperature, wait the temperature of algae mud to reduce to 15-40 ℃, close cooling water intakeoutfall;
3) under stirring state, utilize alkali to regulate pH, make between the algae mud pH 8.0-14.0;
4) under stirring state, add the anti-bad courageous and upright sour cetylate of 3% inhibitor according to algae mud weight ratio, mix;
5) the algae dredge pump is gone in the paulownia body of high pressure homogenizer, the WP of regulating high pressure homogenizer is 120-130Mpa, and the temperature that keeps high pressure homogenizer is carried out high-pressure homogeneous broken wall at 30-95 ℃ with algae mud, and the feed liquid behind the broken wall is directly squeezed in the storage tank of ball mill;
6) pressure of adjusting ball mill is 25Mpa, and controlled temperature carries out secondary broken wall and breakdown of emulsion at 30-95 ℃, and the feed liquid behind the secondary broken wall is packed into and had in the storage tank of gas distribution pipeline and coil pipe;
7) the kettle algae algae mud that splits behind the broken wall is warmed up to 30-95 ℃, under 20-150rpm stirs, heats and kept 0.5-5 hour;
8) ratio by weight Zao Ni ﹕ water=1 ﹕ 2.5-6.5 adds hot water from algae mud bottom through gas distribution pipe; And control is expected warm 30-95 ℃; Add an amount of edible salt and under 250-350rpm stirs, keep 50-80min, feed liquid is separated obtaining the DHA grease through three-phase separator, extraction yield is 94%.
Embodiment 7
1) dino flagellate fermentation liquor after the fermentation ends is utilized separation system separated and collected dino flagellate cell, keep algae mud water cut at 70%-90%; Algae mud is collected in the storage tank that has spiral coil cooling tube and a stirring;
2) be that 10 ℃ of water coolants cool off through in the coil pipe of tank body, feeding temperature, wait the temperature of algae mud to reduce to 20 ℃, close cooling water intakeoutfall;
3) under the high-speed stirring situation, utilize alkali to regulate pH, make between the algae mud pH 8.0-14.0;
4) under the high-speed stirring situation, add 2% antioxidant blends (vitamin E 1.5%, natural Rosmarinus officinalis 0.5%) according to algae mud weight ratio, mix;
5) with in the algae mud colloidal mill, the working speed of regulating colloidal mill is below the 14000rpm, and controlled temperature carries out high-pressure homogeneous broken wall at 30-95 ℃ with algae mud, and the feed liquid behind the broken wall is directly squeezed in the storage tank of ball mill;
6) pressure of adjusting ball mill is 30Mpa, and controlled temperature carries out secondary broken wall and breakdown of emulsion at 30-95 ℃, and the feed liquid behind the secondary broken wall is packed into and had in the storage tank of gas distribution pipeline and coil pipe;
7) the dino flagellate algae mud behind the broken wall is warmed up to 30-95 ℃, under 20-150rpm stirs, heats and kept 0.5-5 hour;
8) ratio by weight Zao Ni ﹕ water=1 ﹕ 2.5-6.5 adds hot water from algae mud bottom through gas distribution pipe; And control is expected warm 30-95 ℃; Add an amount of edible salt and under 250-350rpm stirs, keep 50-80min, feed liquid is separated obtaining the DHA grease through three-phase separator, extraction yield is 91%.
Embodiment 8
1) the Mortierella alpina fermented liquid after the fermentation ends is passed through separation system separated and collected fungal cell, keep cell mud water cut at 70%-90%; Cell mud is collected in the storage tank that has spiral coil cooling tube and a stirring,
2) be that 10 ℃ of water coolants cool off through in the coil pipe of tank body, feeding temperature, wait the temperature of cell mud to reduce to 15-40 ℃, close cooling water intakeoutfall;
3) under stirring state, utilize alkali to regulate pH, make between the cell mud pH 8.0-14.0;
4) under stirring state, add 2.5% antioxidant blends (vitamin E 1%, anti-bad courageous and upright sour cetylate 1.5%) according to cell mud weight ratio, mix;
5) the cell dredge pump is gone in the paulownia body of high pressure homogenizer; The WP of regulating high pressure homogenizer is 120-130Mpa; The temperature that keeps high pressure homogenizer is carried out high-pressure homogeneous broken wall at 30-95 ℃ with cell mud, and the feed liquid behind the broken wall is directly squeezed in the storage tank of ball mill;
6) pressure of adjusting ball mill is 30Mpa, and controlled temperature carries out secondary broken wall and breakdown of emulsion at 30-95 ℃, and the feed liquid behind the secondary broken wall is packed into and had in the storage tank of gas distribution pipeline and coil pipe;
7) the cell mud behind the broken wall is warmed up to 30-95 ℃, under 20-150rpm stirs, heats and kept 0.5-5 hour;
8) ratio by weight cell Ni ﹕ water=1 ﹕ 2.5-6.5 adds hot water from cell mud bottom through gas distribution pipe; And control is expected warm 30-95 ℃; Add an amount of edible salt and under 250-350rpm stirs, keep 50-80min; Feed liquid is obtained pufas ARA grease through the three-phase separator separation, and extraction yield is 85%.
Embodiment 9
1) the Mortierella isabellina fermented liquid after the fermentation ends is passed through separation system separated and collected fungal cell, keep cell mud water cut at 70%-90%; Cell mud is collected in the storage tank that has spiral coil cooling tube and a stirring,
2) be that 10 ℃ of water coolants cool off through in the coil pipe of tank body, feeding temperature, wait the temperature of cell mud to reduce to 15-40 ℃, close cooling water intakeoutfall;
3) under stirring state, utilize alkali to regulate pH, make between the cell mud pH 8.0-14.0;
4) under stirring state, add 1.5% antioxidant blends (vitamin E 1%, natural Rosmarinus officinalis 0.5%) according to cell mud weight ratio and mix;
5) the cell dredge pump is gone in the paulownia body of high pressure homogenizer; The WP of regulating high pressure homogenizer is 120-130Mpa; The temperature that keeps high pressure homogenizer is carried out high-pressure homogeneous broken wall at 30-95 ℃ with cell mud, and the feed liquid behind the broken wall is directly squeezed in the storage tank of ball mill;
6) pressure of adjusting ball mill is 25Mpa, and controlled temperature carries out secondary broken wall and breakdown of emulsion at 30-95 ℃, and the feed liquid behind the secondary broken wall is packed into and had in the storage tank of gas distribution pipeline and coil pipe;
7) the cell mud behind the broken wall is warmed up to 30-95 ℃, under 20-150rpm stirs, heats and kept 0.5-5 hour;
8) ratio by weight cell Ni ﹕ water=1 ﹕ 2.5-6.5 adds hot water from cell mud bottom through gas distribution pipe; And control is expected warm 30-95 ℃; Add an amount of edible salt and under 250-350rpm stirs, keep 50-80min; Feed liquid is obtained pufas EPA grease through the three-phase separator separation, and extraction yield is 85%.
Embodiment 10
1) the little Ke Yinhan fermented liquid after the fermentation ends is passed through separation system separated and collected fungal cell, keep cell mud water cut at 70%-90%; Cell mud is collected in the storage tank that has spiral coil cooling tube and a stirring,
2) be that 10 ℃ of water coolants cool off through in the coil pipe of tank body, feeding temperature, wait the temperature of cell mud to reduce to 15-40 ℃, close cooling water intakeoutfall;
3) under stirring state, utilize alkali to regulate pH, make between the cell mud pH 8.0-14.0;
4) under stirring state, add 3% antioxidant blends (vitamin E 1%, natural Rosmarinus officinalis 0.5%, anti-bad courageous and upright sour cetylate 1.5%) according to cell mud weight ratio, mix;
5) the cell dredge pump is gone in the paulownia body of high pressure homogenizer; The WP of regulating high pressure homogenizer is 120-130Mpa; The temperature that keeps high pressure homogenizer is carried out high-pressure homogeneous broken wall at 30-95 ℃ with cell mud, and the feed liquid behind the broken wall is directly squeezed in the storage tank of ball mill;
6) pressure of adjusting ball mill is 20Mpa, and controlled temperature carries out secondary broken wall and breakdown of emulsion at 30-95 ℃, and the feed liquid behind the secondary broken wall is packed into and had in the storage tank of gas distribution pipeline and coil pipe;
7) the cell mud behind the broken wall is warmed up to 30-95 ℃, under 20-150rpm stirs, heats and kept 0.5-5 hour;
8) ratio by weight cell Ni ﹕ water=1 ﹕ 2.5-6.5 adds hot water from cell mud bottom through gas distribution pipe; And control is expected warm 30-95 ℃; Add an amount of edible salt and under 250-350rpm stirs, keep 50-80min; Feed liquid is obtained pufas GLA and ALA grease through the three-phase separator separation, and extraction yield is 87%.
Although specifically show and introduced the present invention in conjunction with preferred embodiment; But the those skilled in the art should be understood that; In the spirit and scope of the present invention that do not break away from appended claims and limited; In form with on the details the present invention is made various variations, be protection scope of the present invention.
Claims (10)
- One kind from fungi or algae physical wall breaking extract greasy method, it is characterized in that: comprise the steps:Step 1: fungi after the fermentation ends or algae fermented liquid are passed through the separation system collecting cell, keep fungi or frustule mud water cut at 70-90%;Step 2: control cell mud temperature is at 15-40 ℃;Step 3: use acid or alkali to regulate cell mud PH and be 0-5 or 8-14;Step 4: the inhibitor that in cell mud, adds cell shale amount 1-3%;Step 5: the cell dredge pump is gone into colloidal mill or high pressure homogenizer carries out broken wall, and controlled temperature is at 30-95 ℃;Step 6: the cell mud that colloidal mill or high pressure homogenizer come out carries out secondary broken wall and breakdown of emulsion through ball mill, and controlled temperature is at 30-95 ℃;Step 7: the cell mud behind the broken wall is warming up to 30-95 ℃ under the agitation condition of 20-150rpm, and keeps 0.5-5 hour;Step 8: the bottom adds hot water through gas distribution pipe by weight cell Ni ﹕ water=1 ﹕ 2.5-6.5 from cell mud, and controlled temperature 30-95 ℃, stir 50-80min at 250-350rpm, feed liquid is separated obtaining grease through three-phase separator.
- 2. according to claim 1 a kind of from fungi or algae physical wall breaking extract greasy method, it is characterized in that: described in the step 4 inhibitor be vitamin E, natural Rosmarinus officinalis, a kind of in the anti-bad courageous and upright sour cetylate or their mixture.
- 3. according to claim 1 a kind of from fungi or algae physical wall breaking extract greasy method, it is characterized in that: the WP of the high pressure homogenizer described in the step 6 is 120-130Mpa.
- 4. according to claim 1 a kind of from fungi or algae physical wall breaking extract greasy method, it is characterized in that: the working speed of colloidal mill is below the 14000rpm in the step 6.
- 5. according to claim 1 a kind of from fungi or algae physical wall breaking extract greasy method, it is characterized in that: described acid is hydrochloric acid, sulfuric acid, Hydrocerol A or lactic acid, described alkali is sodium hydroxide.
- 6. according to claim 1 a kind of from fungi or algae physical wall breaking extract greasy method, it is characterized in that: described ball mill is sand mill or ball mill, its WP is 20-30Mpa.
- According to the arbitrary claim of claim 1-6 described a kind of from fungi or algae physical wall breaking extract greasy method, it is characterized in that: described algae is for splitting kettle algae or dino flagellate, and grease is the DHA grease.
- According to the arbitrary claim of claim 1-6 described a kind of from fungi or algae physical wall breaking extract greasy method, it is characterized in that: described fungi is a Mortierella alpina, grease is the ARA grease.
- According to the arbitrary claim of claim 1-6 described a kind of from fungi or algae physical wall breaking extract greasy method, it is characterized in that: described fungi is a Mortierella isabellina, grease is the EPA grease.
- According to the arbitrary claim of claim 1-6 described a kind of from fungi or algae physical wall breaking extract greasy method, it is characterized in that: described fungi is little Ke Yinhan, grease is GLA and ALA grease.
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| CN2011102835257A CN102433215A (en) | 2011-09-22 | 2011-09-22 | Method for extracting grease from fungi or algae by physical wall breaking |
| PCT/CN2011/001799 WO2013040732A1 (en) | 2011-09-22 | 2011-10-27 | Method for extracting grease from fungi or alga through physical wall-breaking |
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| CN2011102835257A CN102433215A (en) | 2011-09-22 | 2011-09-22 | Method for extracting grease from fungi or algae by physical wall breaking |
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| CN111690462A (en) * | 2020-06-09 | 2020-09-22 | 厦门汇盛生物有限公司 | Method for demulsifying and extracting oil from oil-containing algae or fungus cell wall-broken liquid |
| CN111763698A (en) * | 2020-06-19 | 2020-10-13 | 山东天智绿业生物科技有限公司 | Chlorella fermentation method for preparing and producing EPA and EPA extraction method |
| CN111748478A (en) * | 2020-06-28 | 2020-10-09 | 深圳大学 | A kind of microalgae wall-breaking processing method and its application |
| CN111748478B (en) * | 2020-06-28 | 2023-02-03 | 深圳大学 | Microalgae wall-breaking processing method and application thereof |
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