CN102342541A - Method for reducing content of fluorine in enzymatic hydrolysate of Euphausia superb by using calcined lime - Google Patents
Method for reducing content of fluorine in enzymatic hydrolysate of Euphausia superb by using calcined lime Download PDFInfo
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- CN102342541A CN102342541A CN201110259184XA CN201110259184A CN102342541A CN 102342541 A CN102342541 A CN 102342541A CN 201110259184X A CN201110259184X A CN 201110259184XA CN 201110259184 A CN201110259184 A CN 201110259184A CN 102342541 A CN102342541 A CN 102342541A
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- Prior art keywords
- krill
- quick lime
- enzymolysis liquid
- fluorine content
- fluorine
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- 239000011737 fluorine Substances 0.000 title claims abstract description 39
- 229910052731 fluorine Inorganic materials 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims abstract description 26
- 241000239368 Euphausia Species 0.000 title abstract 7
- 230000002255 enzymatic effect Effects 0.000 title abstract 6
- 235000008733 Citrus aurantifolia Nutrition 0.000 title abstract 5
- 235000011941 Tilia x europaea Nutrition 0.000 title abstract 5
- 239000000413 hydrolysate Substances 0.000 title abstract 5
- 239000004571 lime Substances 0.000 title abstract 5
- PXGOKWXKJXAPGV-UHFFFAOYSA-N Fluorine Chemical compound FF PXGOKWXKJXAPGV-UHFFFAOYSA-N 0.000 title 1
- YCKRFDGAMUMZLT-UHFFFAOYSA-N Fluorine atom Chemical compound [F] YCKRFDGAMUMZLT-UHFFFAOYSA-N 0.000 claims abstract description 38
- 102000004190 Enzymes Human genes 0.000 claims abstract description 14
- 108090000790 Enzymes Proteins 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000012153 distilled water Substances 0.000 claims abstract description 9
- 108010056079 Subtilisins Proteins 0.000 claims abstract description 5
- 238000003756 stirring Methods 0.000 claims abstract description 5
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims description 65
- 241000239366 Euphausiacea Species 0.000 claims description 53
- 239000000292 calcium oxide Substances 0.000 claims description 33
- 235000012255 calcium oxide Nutrition 0.000 claims description 33
- 239000007788 liquid Substances 0.000 claims description 26
- 238000006243 chemical reaction Methods 0.000 claims description 7
- 239000012528 membrane Substances 0.000 claims description 5
- 241000238557 Decapoda Species 0.000 claims description 4
- 239000002002 slurry Substances 0.000 claims description 4
- 229910052799 carbon Inorganic materials 0.000 claims description 3
- 238000007710 freezing Methods 0.000 claims description 3
- 230000008014 freezing Effects 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 7
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- 108010009736 Protein Hydrolysates Proteins 0.000 abstract 1
- 230000007071 enzymatic hydrolysis Effects 0.000 abstract 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 abstract 1
- 239000000706 filtrate Substances 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 238000005360 mashing Methods 0.000 abstract 1
- 239000000047 product Substances 0.000 abstract 1
- 239000000376 reactant Substances 0.000 description 6
- KRHYYFGTRYWZRS-UHFFFAOYSA-M Fluoride anion Chemical compound [F-] KRHYYFGTRYWZRS-UHFFFAOYSA-M 0.000 description 5
- 230000009286 beneficial effect Effects 0.000 description 4
- 238000005342 ion exchange Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 125000001153 fluoro group Chemical group F* 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 230000004308 accommodation Effects 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 238000009388 chemical precipitation Methods 0.000 description 1
- 238000005868 electrolysis reaction Methods 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
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- Coloring Foods And Improving Nutritive Qualities (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The invention relates to a method for reducing the content of fluorine in enzymatic hydrolysate of Euphausia superb by using calcined lime, comprising the following steps of: (1) mashing the Euphausia superb, then adding distilled water for homogenating to obtain Euphausia superb pulp, and then adding Alcalase protease, carrying out enzymatic hydrolysis under the conditions with a temperature of 50-55 DEG C and a pH value of 8.0-8.5 for 220-260min, and finally killing enzyme and centrifuging to obtain enzymatic hydrolysate of the Euphausia superb; and (2) adding calcined lime with the concentration of 8-12g/L into the enzymatic hydrolysate, and stirring under the condition with the pH value of 10.0-11.5 for 40-50min; and finally filtering to obtain filtrate, i.e., the enzymatic hydrolysate of the Euphausia superb, which has reduced content of fluorine. In the invention, the calcined lime as the raw material is low in price and wide in source; and the method has a good fluorine reduction effect without lowering the nutritional value of the enzymatic hydrolysate of the Euphausia superb, and can be used for completely removing products and remained calcined lime simply after fluorine reduction, thereby having a wide application prospect.
Description
Technical field
The invention belongs to the method field that reduces fluorine content in the solution, particularly a kind of method of utilizing quick lime to reduce fluorine content in the krill enzymolysis liquid.
Background technology
The krill resource is very abundant, and nutritious, protein, fat, mineral element equal size height, and therefore as human new resource for food, development and utilization has a high potential.The krill body contains a large amount of fluorine, and the whole krill fluorine content that people such as Germany scientist Soevik record freezing storage is about 2400mg/kg.An amount of fluorine helps the growth of human skeletal and tooth, but the fluorine of excess intake can cause skeleton deformity.The World Health Organization (WHO) regulation adult is the highest to be taken the photograph fluorine and limits the quantity of and be not higher than 4mg/d, this shows the accommodation of fluorine superman's body far away of krill, and the food of directly edible krill exploitation can influence health.Therefore realize the edibleization of krill product, must reduce the fluorine content in the product.
The method that reduces fluorine content in the solution at present has a lot, mainly contains chemical precipitation method, ion-exchange, filter membrane method, electrolysis and absorption method.In these methods, the absorption method with high efficiency and ease for operation is the most frequently used, has developed different types of adsorbent at present, for example activated alumina, bone carbon, synthetic ion exchange material etc.The adsorption reaction process is meant that fluorine ion passes through the ion-exchange or the surface chemical reaction of boundary layer.But a lot of reducing fluoride methods are with instrument is mostly expensive or the operation inconvenient; And contain a large amount of nutritional labelings and active material in the krill enzymolysis liquid; Require to influence the trophic function of original enzymolysis liquid when reducing fluorine content wherein, it is economical convenient and to fall the high reducing fluoride method of fluorine efficient very important therefore to select.
Summary of the invention
Technical problem to be solved by this invention provides a kind of method of utilizing quick lime to reduce fluorine content in the krill enzymolysis liquid, and this method is simple to operate, and cost is low, and fluoro droping effect is good, is beneficial in actual production and promotes the use of.
A kind of method of utilizing quick lime to reduce fluorine content in the krill enzymolysis liquid of the present invention comprises:
(1) krill is smashed to pieces; And then adding distilled water homogenate; Get krill shrimp slurry; Add Alcalase protease (actual measurement enzyme activity 109387U/g purchases the Bioisystech Co., Ltd in Novozyme (China)) then, enzyme concentration is 3000-3030U/g; At 50-55 ℃; The pH value is enzymolysis 220-260min under the condition of 8.0-8.5, the enzyme that goes out at last, centrifugal krill enzymolysis liquid; Wherein the volume ratio of krill and distilled water is 1: 2-1: 4;
(2) by the concentration of 8-12g/L quick lime is added in the enzymolysis liquid, stir 40-50min under the condition of 10.0-11.5 in the pH value; Filter at last, filtrating is the krill hydrolyzate that has reduced fluorine content.
Krill described in the step (1) is freezing krill.
The enzyme that goes out described in the step (1) is at 90 ℃ of water bath heat preservation 10min.
Centrifugal rotation speed described in the step (1) is 11000r/min, and the time is 15min.
Through handling, processing procedure is quick lime described in the step (2) in advance: quick lime is ground, cross 120 mesh standard sieves, behind baking 4h under 90～105 ℃, in drier, store for future use then.
Being filtered into described in the step (2): adopting diameter is the filter membrane of 0.40 μ m, filters the product and residue quick lime of removing reaction.
Because solution was the solid-liquid mixed system after the fluorine ion reaction finished in quick lime and the krill hydrolyzate, for product and the residue quick lime of realizing the edible of krill hydrolyzate, must remove suspending in this system.
The present invention is adsorbed the fluorine in the krill enzymolysis liquid with quick lime through ion-exchange reactions, through filter membrane reactant and residue quick lime are all removed then.
Advantage of the present invention is that the quick lime fluoro droping effect is good; Krill enzymolysis liquid fluorine content through falling after fluorine is handled reaches about 2.0mg/kg; And low price; Fall the fluorine process and can not reduce the nutritive value of krill enzymolysis liquid; Can simply reactant and residue quick lime all be removed after falling fluorine, be beneficial in actual production and promote the use of.
Beneficial effect:
(1) raw material of the present invention is a quick lime, its low price, and wide material sources, therefore, cost of the present invention is relatively low;
(2) the present invention adopts quick lime to fall fluorine, and its fluoro droping effect is good, and can not reduce the nutritive value of krill enzymolysis liquid, falls behind the fluorine and can simply reactant and residue quick lime all be removed, and is beneficial in actual production and promotes the use of;
(3) fluorine content is about 2.0mg/kg in the resulting krill hydrolyzate that reduces fluorine content of the present invention; Improved the intake of krill greatly; This is the edible healthily krills of the mankind, and development and utilization krill resource, has made contribution.
The specific embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in the restriction scope of the present invention.Should be understood that in addition those skilled in the art can do various changes or modification to the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Embodiment 1
(1) krill is smashed to pieces; And then adding distilled water homogenate; Get krill shrimp slurry, add Alcalase protease (actual measurement enzyme activity 109387U/g purchases the Bioisystech Co., Ltd in Novozyme (China)) then; Enzyme concentration is 3010U/g; At 50 ℃, the pH value is enzymolysis 240min under 8.0 the condition, then in 90 ℃ of water bath heat preservation 10min enzyme that goes out; It is centrifugal at last that (11000r/min 15min) gets the krill enzymolysis liquid; Wherein the volume ratio of krill and distilled water is 1: 3;
(2) quick lime is ground, cross 120 mesh standard sieves, in drier, store for future use behind the oven dry 4h down in 90～105 ℃; The quick lime addition is the 10g/L enzymolysis liquid, under pH 11.5 conditions, stirs 50min; At last, remove reactant, utilizing diameter is the filter membrane of 0.40 μ m, filters and removes reacted reactant and residue quick lime, and filtrating is the krill hydrolyzate that has reduced fluorine content; Because solution was the solid-liquid mixed system after the fluorine ion reaction finished in quick lime and the krill hydrolyzate, for reactant and the residue quick lime of realizing the edible of krill hydrolyzate, must remove suspending in this system.
Embodiment 2
(1) krill is smashed to pieces; And then adding distilled water homogenate; Get krill shrimp slurry; Add Alcalase protease (actual measurement enzyme activity 109387U/g purchases the Bioisystech Co., Ltd in Novozyme (China)) then, enzyme concentration is 3000U/g; At 53 ℃; The pH value is enzymolysis 220min under 8.2 the condition, the enzyme that goes out at last, centrifugal krill enzymolysis liquid; Wherein the volume ratio of krill and distilled water is 1: 4;
(2) by the concentration of 8g/L quick lime being added in the enzymolysis liquid, is to stir 45min under 11.0 the condition in the pH value; Filter at last, filtrating is the krill hydrolyzate that has reduced fluorine content.
Claims (6)
1. method of utilizing quick lime to reduce fluorine content in the krill enzymolysis liquid comprises:
(1) krill is smashed to pieces, and then added distilled water homogenate, get krill shrimp slurry; Add Alcalase protease then, enzyme concentration is 3000-3030U/g, at 50-55 ℃; The pH value is enzymolysis 220-260min under the condition of 8.0-8.5, the enzyme that goes out at last, centrifugal krill enzymolysis liquid; Wherein the volume ratio of krill and distilled water is 1: 2-1: 4;
(2) by the concentration of 8-12g/L quick lime is added in the enzymolysis liquid, stir 40-50min under the condition of 10.0-11.5 in the pH value; Filter at last, filtrating is the krill hydrolyzate that has reduced fluorine content.
2. a kind of method of utilizing quick lime to reduce fluorine content in the krill enzymolysis liquid according to claim 1, it is characterized in that: the krill described in the step (1) is freezing krill.
3. a kind of method of utilizing quick lime to reduce fluorine content in the krill enzymolysis liquid according to claim 1 is characterized in that: the enzyme that goes out described in the step (1) is at 90 ℃ of water bath heat preservation 10min.
4. a kind of method of utilizing quick lime to reduce fluorine content in the krill enzymolysis liquid according to claim 1, it is characterized in that: the centrifugal rotation speed described in the step (1) is 11000r/min, the time is 15min.
5. a kind of method of utilizing quick lime to reduce fluorine content in the krill enzymolysis liquid according to claim 1; It is characterized in that: the quick lime described in the step (2) is in advance through handling; Processing procedure is: quick lime is ground; Cross 120 mesh standard sieves; Behind baking 4h under 90～105 ℃, in drier, store for future use then.
6. a kind of method of utilizing quick lime to reduce fluorine content in the krill enzymolysis liquid according to claim 1; It is characterized in that: being filtered into described in the step (2): adopting diameter is the filter membrane of 0.40 μ m, filters the product and residue quick lime of removing reaction.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201110259184XA CN102342541B (en) | 2011-09-02 | 2011-09-02 | Method for reducing content of fluorine in enzymatic hydrolysate of Euphausia superb by using calcined lime |
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| CN201110259184XA CN102342541B (en) | 2011-09-02 | 2011-09-02 | Method for reducing content of fluorine in enzymatic hydrolysate of Euphausia superb by using calcined lime |
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| CN102342541B CN102342541B (en) | 2013-02-20 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103238723A (en) * | 2012-02-10 | 2013-08-14 | 上海市水产研究所 | Preparation method of low-fluorine euphausia superb hydrolyzed protein powder |
| CN103583931A (en) * | 2013-11-11 | 2014-02-19 | 山东师范大学 | Ultrasonic defluorination method for whole euphausia superba |
| CN104232717A (en) * | 2014-09-12 | 2014-12-24 | 中国海洋大学 | Method for reducing fluorine content in water soluble active peptides from Antarctic krill |
| CN105039475A (en) * | 2015-06-26 | 2015-11-11 | 浙江海洋学院 | Method for removing fluorine in euphausia superba enzymatic hydrolysate |
| CN105419936A (en) * | 2015-11-04 | 2016-03-23 | 大连工业大学 | Preparation method of low fluorine content Euphausia superba oil |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102170795A (en) * | 2008-09-12 | 2011-08-31 | 翡翠渔业公司 | Process for reducing the fluoride content when producing proteinaceous concentrates from krill |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102170795A (en) * | 2008-09-12 | 2011-08-31 | 翡翠渔业公司 | Process for reducing the fluoride content when producing proteinaceous concentrates from krill |
Non-Patent Citations (2)
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| 张希祥: "生石灰处理高浓度含氟废水的研究", 《核电工程与技术》, vol. 14, no. 1, 31 March 2001 (2001-03-31), pages 45 - 48 * |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103238723A (en) * | 2012-02-10 | 2013-08-14 | 上海市水产研究所 | Preparation method of low-fluorine euphausia superb hydrolyzed protein powder |
| CN103238723B (en) * | 2012-02-10 | 2014-10-29 | 上海市水产研究所 | Preparation method of low-fluorine euphausia superb hydrolyzed protein powder |
| CN103583931A (en) * | 2013-11-11 | 2014-02-19 | 山东师范大学 | Ultrasonic defluorination method for whole euphausia superba |
| CN103583931B (en) * | 2013-11-11 | 2015-05-27 | 山东师范大学 | Ultrasonic defluorination method for whole euphausia superba |
| CN104232717A (en) * | 2014-09-12 | 2014-12-24 | 中国海洋大学 | Method for reducing fluorine content in water soluble active peptides from Antarctic krill |
| CN105039475A (en) * | 2015-06-26 | 2015-11-11 | 浙江海洋学院 | Method for removing fluorine in euphausia superba enzymatic hydrolysate |
| CN105419936A (en) * | 2015-11-04 | 2016-03-23 | 大连工业大学 | Preparation method of low fluorine content Euphausia superba oil |
| CN105419936B (en) * | 2015-11-04 | 2020-04-10 | 大连工业大学 | Preparation method of euphausia superba oil with low fluorine content |
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| CN102342541B (en) | 2013-02-20 |
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Address after: 200090 Shanghai military road, Yangpu District, No. 300 Applicant after: East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences Address before: 200090 No. 100, military road, Shanghai, Yangpu District Applicant before: East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences |
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