CN102217563A - Nucleus inserting method for nuclear-free pearl grain breeding - Google Patents
Nucleus inserting method for nuclear-free pearl grain breeding Download PDFInfo
- Publication number
- CN102217563A CN102217563A CN2011100999356A CN201110099935A CN102217563A CN 102217563 A CN102217563 A CN 102217563A CN 2011100999356 A CN2011100999356 A CN 2011100999356A CN 201110099935 A CN201110099935 A CN 201110099935A CN 102217563 A CN102217563 A CN 102217563A
- Authority
- CN
- China
- Prior art keywords
- pearl
- powder
- solution
- nucleus
- shells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Farming Of Fish And Shellfish (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to a nucleus inserting method for nuclear-free pearl grain breeding. The method comprises the following steps of: taking pearl matrix of seawater pearl, crushing, sieving, taking particles with average particle diameter of 5 to 50 microns, and sterilizing for later use; taking thickening agent and sterile water to prepare solution, heating, sterilizing, and adding pearl matrix powder to prepare solution containing pearl powder; and selecting healthy pearl breeding shells, opening the pearl shells by a pearl inserting method, sucking the prepared pearl powder solution by using an injector, injecting the pearl powder solution to the partial tissue organs of the fertile pearls in the pearl shells, culturing the pearl breeding shells injected with the pearl powder solution by a conventional pearl shell culture method, and harvesting pearls after the pearl breeding shells are cultured for 6 to 36 months. The nucleus inserting method is simple and efficient, the bred pearl is nuclear-free and totally can be edible or officinal or used for cosmetics, the problem of edible pearl raw material source is solved, and an effective path is provided for the development of the pearl industry.
Description
Technical field
The invention belongs to the pearl cultivating technical field, relate to a kind of nuclear insertion method of cultivation of the no nucleus pearl grain that is used for medical treatment, food or cosmetics, specifically is the nuclear insertion method that a kind of tiny seawater does not have the cultivation of nucleus pearl grain.
Background technology
Pearl is a kind of famous and precious traditional Chinese medicine, have calm the nerves, make eye bright, the effect of detoxifcation and beauty and health care.Modern science the analysis showed that pearl contains nearly 20 seed amino acids, 20 various trace elements, porphyrin and abundant calcium class.The natural quantity of producing of sea water pearls grain is few; and be of great rarity; the pearl grain of artificial culture contains pearl nucleus substantially; pearl nucleus are generally the fresh water shell and make; be non-nacre composition, because non-nacre composition occupies ratio in the pearl grain of artificial culture very big, the proportion that accounts for the pearl grain is more than 90%; can not satisfy the needs of producing and using, and the output of the natural no nucleus pearl grain of seawater can not be satisfied with people's demand far away.
Along with the improvement of people life, for the needs of beauty and health care, the consumption figure of pearl is increasing, and the sea water pearls output that can be used for health and beauty is not seen growth, and therefore, cultivating the edible sea water pearls has become problem demanding prompt solution.By retrieval, have not yet to see the bibliographical information of the edible sea water pearls of special cultivation.
Summary of the invention
The purpose of this invention is to provide a kind of nuclear insertion method that the nucleus pearl grain is cultivated that do not have, be with small sea water pearls matter powder, be made into the position of growing cultured pearls of injecting pearl culturing clam behind the suspension with thickener and sterile water, form the nacre nuclear identical, thereby cultivate excellent edible sea water pearls grain with the nacre composition.
The technical solution adopted in the present invention:
A kind of nuclear insertion method that does not have the cultivation of nucleus pearl grain comprises following concrete steps:
1, with extracting nacre after the sea water pearls fragmentation, nacre is pulverized at a high speed, sieved, be averaged particle diameter at 5～50 microns particulate, the steam sterilization is standby; Getting thickener and sterile water, to be mixed with mass percent be 0.1～0.5% solution, and heat sterilization adds the nacre powder of sterilizing, and stirs, and is mixed with to contain pearl powder 0.1～10% solution.Described thickener is medical gelatin, hyaluronic acid, medical chitin or xanthan gum.
2, the healthy pearly shellfish of choosing is opened pearl shell by slotting pearl method, draws the pearl powder solution for preparing with syringe, injects the portion of tissue organ that pearl shell can be grown cultured pearls, each 1～10.0 microlitre; Every Becquerel is to inject 20～30 times.In order to alleviate working strength, also available continuous dispensing device (AUTOREP) as the electronic multichannel pipettor of plum Teller EDP3, contains 8 separating tubes, the separatory pipe end connects the about 1-3 millimeter pvc pipe line in footpath, another termination of pipeline 3-9 syringe needle, 8 syringe needles are integrated a branch of, are separated by about 2～5 millimeters between every syringe needle, set the automatic or manual application of sample, 8 samples of every batch of injectable, the about 1-10 microlitre of each sample, 24-48 sample of each Becquerel injection.Choose the position of growing cultured pearls, needle tubing is inserted in the tissue of growing cultured pearls, injection once can be injected 8 samples.
3, pearl breedinmg method is routinely cultured the pearly shellfish that injects pearl powder solution, and a no nucleus pearl can be grown in each injection site, cultures after 6-36 month and can receive Bei Caizhu.The heavily about 0.2-3.5g of each pearl does not wait.General each Becquerel 2-12g pearl grain of gathering.
Nuclear insertion method of the present invention is simple, the efficient height, and the pearl of cultivating seedless (promptly not containing non-nacre composition), fully edible or medicinal, used for cosmetic has solved edible pearl raw material sources problem, for the development of pearl industry provides an effective way.
Embodiment
Below in conjunction with embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.
Embodiment one
(1), getting sea water pearls 500g is broken into 3-20 order coarse granule (pearl nucleus is frangible, be broken into thinner particle, meal is crossed 30 purposes sieve, coarse granule is not sieved, the coarse granule main component of not sieving is the nacre with certain toughness), the particle that pearl nucleus are arranged is sticked in removal, and remaining coarse granule is pure nacre 43g, and nacre is pulverized with high speed disintegrator, cross 120 mesh sieves, obtain 120 order pearl powders, do not cross 120 purposes and continue to pulverize, till crossing 120 orders.Gained pearl powder 42g (loss 1g) water steam sterilization is standby; Select for use medical gelatin 42g to add clean sterile water 21kg and be mixed with 0.2% gelatin and mix liquid, heat sterilization adds the pearl powder 42g that sterilize, stirs, and is mixed with to contain pearl powder 0.2% mixed liquid.
1000 of the pearly shellfishes of (2), choosing health, open pearl shell by slotting pearl method, with the electronic multichannel pipettor of plum Teller EDP3, pack into and contain the mixed liquid of pearl powder 0.2%, each dress 5ml, multichannel pipettor contains 8 separating tubes, the separatory pipe end connects the about 1 millimeter pvc pipe line in footpath, another termination of pipeline 3-9 syringe needle, and 8 syringe needles are integrated a branch of, about 2-5 millimeter of being separated by between every syringe needle, set the automatic or manual application of sample, 8 samples of every batch of injectable, about 2.5 microlitres of each sample, 20-30 sample of each shellfish injection (decide according to the individual size of shellfish, big Becquerel is some more to inject).
(3), grow cultured pearls.Pearl breedinmg method is routinely cultured, and each sample can be grown a no nucleus pearl.Culture and receive Bei Caizhu after 24 months.The heavily about 0.2-3.5g of each pearl does not wait.General each Becquerel 2-10g pearl grain of gathering.1000 shellfishes are removed 113 of shellfishes dead in the breeding process, altogether 887 of the shellfishes of living, adopt no nucleus pearl 5630g.
Embodiment two
(1), get sea water pearls 800g and be broken into 3-20 order coarse granule, remove and stick the particle that pearl nucleus are arranged, remaining coarse granule is pure nacre 75g, nacre is pulverized with high speed disintegrator, crossed 120 mesh sieves, obtain 120 order pearl powders, do not cross 120 purposes and continue to pulverize, till crossing 120 orders.Gained pearl powder 73g (loss 2g) water steam sterilization is standby; Select for use hyaluronic acid 16g to add clean sterile water 4kg and be mixed with 0.4% hyaluronic acid and mix liquid, heat sterilization adds the pearl powder 45g that sterilize, stirs, and is mixed with to contain pearl powder 1.8% mixed liquid.
500 of the pearly shellfishes of (2), choosing health, open pearl shell by slotting pearl method, with the electronic multichannel pipettor of plum Teller EDP3, pack into and contain the mixed liquid of pearl powder 1.2%, each dress 5ml, multichannel pipettor contains 8 separating tubes, the separatory pipe end connects the about 1 millimeter pvc pipe line in footpath, another termination of pipeline 3-9 syringe needle, and 8 syringe needles are integrated a branch of, about 2-5 millimeter of being separated by between every syringe needle, set the automatic or manual application of sample, 8 samples of every batch of injectable, about 1.5 microlitres of each sample, 20-30 sample of each shellfish injection (decide according to the individual size of shellfish, big Becquerel is some more to inject).
(3), grow cultured pearls.Pearl breedinmg method is routinely cultured, and each sample can be grown a no nucleus pearl.Culture and receive Bei Caizhu after 30 months.The heavily about 0.5-3.5g of each pearl does not wait.General each Becquerel 8-12g pearl grain of gathering.500 shellfishes are removed 167 of shellfishes dead in the breeding process, altogether 333 of the shellfishes of living, adopt no nucleus pearl 3430g.
Embodiment three
(1), gets sea water pearls 1000g and be broken into 3-20 order coarse granule, the particle that pearl nucleus are arranged is sticked in removal, remaining coarse granule is pure nacre 96g, nacre is pulverized with high speed disintegrator, cross 120 mesh sieves, obtain 120 order pearl powders, do not cross 120 purposes and continue to pulverize, till crossing 120 orders.Gained pearl powder 95g (loss 1g) water steam sterilization is standby; Select for use medical gelatin 9g to add clean sterile water 3kg and be mixed with 0.3% gelatin and mix liquid, heat sterilization adds the pearl powder 95g that sterilize, stirs, and is mixed with to contain pearl powder 3.2% mixed liquid.
500 of the pearly shellfishes of (2), choosing health, open pearl shell by slotting pearl method, with the electronic multichannel pipettor of plum Teller EDP3, the 3.2% mixed liquid that contains pearl powder of packing into, each dress 5ml, multichannel pipettor contains 8 separating tubes, the separatory pipe end connects the about 1 millimeter pvc pipe line in footpath, another termination of pipeline 3-9 syringe needle, and 8 syringe needles are integrated a branch of, about 2-5 millimeter of being separated by between every syringe needle, set the automatic or manual application of sample, 8 samples of every batch of injectable, about 1.5 microlitres of each sample, 20-30 sample of each shellfish injection (decide according to the individual size of shellfish, big Becquerel is some more to inject).
(3), grow cultured pearls.Pearl breedinmg method is routinely cultured, and each sample can be grown a no nucleus pearl.Culture and receive Bei Caizhu after 10 months.The heavily about 0.2-2.5g of each pearl does not wait.General each Becquerel 2-9g pearl grain of gathering.500 shellfishes are removed 83 of shellfishes dead in the breeding process, altogether 417 of the shellfishes of living, adopt no nucleus pearl 2340g.
Claims (2)
1. one kind is not had the nuclear insertion method that the nucleus pearl grain is cultivated, and it is characterized in that, comprises following concrete steps:
1), with extracting nacre after the sea water pearls fragmentation, nacre is pulverized at a high speed, sieve, be averaged particle diameter at 5～50 microns particulate, the steam sterilization is standby; Getting thickener and sterile water, to be mixed with mass percent be 0.1～0.5% solution, and heat sterilization adds the nacre powder of sterilizing, and stirs, and is mixed with to contain pearl powder 0.1～10% solution; Described thickener is medical gelatin, hyaluronic acid, medical chitin or xanthan gum;
2), the healthy pearly shellfish of choosing, open pearl shell by slotting pearl method, draw the pearl powder solution for preparing with syringe, the portion of tissue organ that the injection pearl shell can be grown cultured pearls, each 1～10.0 microlitre;
3), pearl breedinmg method routinely culture to inject the pearly shellfish of pearl powder solution, culture after 6-36 month and can receive Bei Caizhu.
2. the nuclear insertion method that no nucleus pearl grain according to claim 1 is cultivated is characterized in that: the pearl powder solution that every pearly shellfish prepares with injector to inject 20～30 times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110099935A CN102217563B (en) | 2011-04-11 | 2011-04-11 | Nucleus inserting method for nuclear-free pearl grain breeding |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110099935A CN102217563B (en) | 2011-04-11 | 2011-04-11 | Nucleus inserting method for nuclear-free pearl grain breeding |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102217563A true CN102217563A (en) | 2011-10-19 |
| CN102217563B CN102217563B (en) | 2012-10-03 |
Family
ID=44774478
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110099935A Active CN102217563B (en) | 2011-04-11 | 2011-04-11 | Nucleus inserting method for nuclear-free pearl grain breeding |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102217563B (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102845344A (en) * | 2012-09-25 | 2013-01-02 | 中国水产科学研究院南海水产研究所热带水产研究开发中心 | Guiding and positioning operation tool for nucleus insertion of seawater pearl shell |
| CN104957066A (en) * | 2015-07-09 | 2015-10-07 | 海南大学 | One-body-three-use method for cultivating three types of pearls by means of pteria penguin |
| CN108935249A (en) * | 2018-08-03 | 2018-12-07 | 广东荣辉珍珠养殖有限公司 | Pearl breedinmg natural peral method and gained pearl |
| CN109601445A (en) * | 2019-01-02 | 2019-04-12 | 广东海洋大学 | A kind of cultivation method for improving pearl production of Pinctada martensii particles |
| CN110772543A (en) * | 2019-11-12 | 2020-02-11 | 浙江长生鸟健康科技股份有限公司 | Preparation process of superfine pearl powder |
| CN111466324A (en) * | 2020-05-05 | 2020-07-31 | 北海市源龙珍珠有限公司 | Method for cultivating seawater pearls by using pearl cell sap |
| CN111466325A (en) * | 2020-05-05 | 2020-07-31 | 北海市源龙珍珠有限公司 | Pearl cell liquid for cultivating seawater pearls |
| CN113207771A (en) * | 2021-05-08 | 2021-08-06 | 北海市源龙珍珠有限公司 | Multiple amino acid calcium culture solution for culturing pearls |
| CN113207770A (en) * | 2021-05-08 | 2021-08-06 | 北海市源龙珍珠有限公司 | Method for cultivating pearls by using various calcium amino acids |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05219856A (en) * | 1991-10-03 | 1993-08-31 | Eisai Co Ltd | Method for culturing pearl by inserting drug-containing solid material |
| JPH10108581A (en) * | 1996-08-13 | 1998-04-28 | Noriko Uehara | Pearl culture method |
| JPH10108582A (en) * | 1996-10-08 | 1998-04-28 | Tazaki Shinjiyu Kk | Nucleus insertion method for cultured pearl |
| CN1294844A (en) * | 2000-06-20 | 2001-05-16 | 澄海市新溪珍珠养殖场 | Pearl seed inserting method for pearl culture in fresh water mussel |
| CN101491228A (en) * | 2009-03-02 | 2009-07-29 | 广西中医学院 | Sea no-nucleus pearl incubation method |
| CN101653103A (en) * | 2009-09-05 | 2010-02-24 | 湖南文理学院 | Breeding method of ultra-large nuclear-free pearl with good quality |
-
2011
- 2011-04-11 CN CN201110099935A patent/CN102217563B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05219856A (en) * | 1991-10-03 | 1993-08-31 | Eisai Co Ltd | Method for culturing pearl by inserting drug-containing solid material |
| JPH10108581A (en) * | 1996-08-13 | 1998-04-28 | Noriko Uehara | Pearl culture method |
| JPH10108582A (en) * | 1996-10-08 | 1998-04-28 | Tazaki Shinjiyu Kk | Nucleus insertion method for cultured pearl |
| CN1294844A (en) * | 2000-06-20 | 2001-05-16 | 澄海市新溪珍珠养殖场 | Pearl seed inserting method for pearl culture in fresh water mussel |
| CN101491228A (en) * | 2009-03-02 | 2009-07-29 | 广西中医学院 | Sea no-nucleus pearl incubation method |
| CN101653103A (en) * | 2009-09-05 | 2010-02-24 | 湖南文理学院 | Breeding method of ultra-large nuclear-free pearl with good quality |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102845344A (en) * | 2012-09-25 | 2013-01-02 | 中国水产科学研究院南海水产研究所热带水产研究开发中心 | Guiding and positioning operation tool for nucleus insertion of seawater pearl shell |
| CN104957066A (en) * | 2015-07-09 | 2015-10-07 | 海南大学 | One-body-three-use method for cultivating three types of pearls by means of pteria penguin |
| CN108935249A (en) * | 2018-08-03 | 2018-12-07 | 广东荣辉珍珠养殖有限公司 | Pearl breedinmg natural peral method and gained pearl |
| CN108935249B (en) * | 2018-08-03 | 2021-03-19 | 广东荣辉珍珠养殖有限公司 | Method for cultivating natural pearl by pearl shell and pearl obtained thereby |
| CN109601445A (en) * | 2019-01-02 | 2019-04-12 | 广东海洋大学 | A kind of cultivation method for improving pearl production of Pinctada martensii particles |
| CN110772543A (en) * | 2019-11-12 | 2020-02-11 | 浙江长生鸟健康科技股份有限公司 | Preparation process of superfine pearl powder |
| CN110772543B (en) * | 2019-11-12 | 2023-04-18 | 浙江长生鸟健康科技股份有限公司 | Preparation process of superfine pearl powder |
| CN111466324A (en) * | 2020-05-05 | 2020-07-31 | 北海市源龙珍珠有限公司 | Method for cultivating seawater pearls by using pearl cell sap |
| CN111466325A (en) * | 2020-05-05 | 2020-07-31 | 北海市源龙珍珠有限公司 | Pearl cell liquid for cultivating seawater pearls |
| CN113207771A (en) * | 2021-05-08 | 2021-08-06 | 北海市源龙珍珠有限公司 | Multiple amino acid calcium culture solution for culturing pearls |
| CN113207770A (en) * | 2021-05-08 | 2021-08-06 | 北海市源龙珍珠有限公司 | Method for cultivating pearls by using various calcium amino acids |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102217563B (en) | 2012-10-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102217563B (en) | Nucleus inserting method for nuclear-free pearl grain breeding | |
| CN101491228B (en) | Sea no-nucleus pearl incubation method | |
| CN102715105B (en) | Industrialization cultivation method for shrimp seed of macrobrachium rosenbergii | |
| CN108935249B (en) | Method for cultivating natural pearl by pearl shell and pearl obtained thereby | |
| CN101215527A (en) | Method for cultivating silkworm chrysalis Cordyceps sinensis | |
| CN102177868B (en) | Method for pond three-dimensional (3D) culture of Chinese prawns | |
| CN103478033B (en) | Nuisanceless eel farming method | |
| CN105687245A (en) | Method of preparing injection solution | |
| CN104498433A (en) | Extraction method of adipose-derived stem cells as well as preparation and application of adipose-derived stem cells | |
| CN103100112A (en) | Preparation method and use of graft material in double membrane structure | |
| CN104664170A (en) | Feed additive for preventing fatty liver of largemouth bass | |
| CN109601445A (en) | A kind of cultivation method for improving pearl production of Pinctada martensii particles | |
| CN102934620B (en) | Method for feeding sepiella maindroni | |
| CN106727810A (en) | A kind of nano particle in ginseng source and its preparation and application | |
| CN104686311A (en) | Roxburgh anoectochilus terminal bud seedling transplantation culture medium | |
| CN105454659A (en) | Carp pond culture method by use of Chinese herbal medicine | |
| CN101492644B (en) | A method for biosynthesizing 15N labeled spirulina | |
| CN103563806B (en) | Cultivation method capable of reducing stichopus japonicus disease and pest injuries with corrosion inhibition nanometer materials | |
| CN102321569B (en) | Method for constructing Kareius bicoloratus liver cell line | |
| CN104920271A (en) | Pteria penguin granule cultivation method and device adopted in same | |
| CN101946812A (en) | Guava leaf and new application of extract thereof | |
| CN205867721U (en) | Garrulous liquid filtering sterilizing system of spirulina powder | |
| CN105400737A (en) | Preparation method of regeneration and repair cells for treating cerebellar atrophy | |
| CN105746390A (en) | Family pond ecological culture method for mandarin fish | |
| CN104818246B (en) | A kind of isolated culture method of rabbit fat stem cell |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Nucleus inserting method for nuclear-free pearl grain breeding Effective date of registration: 20160518 Granted publication date: 20121003 Pledgee: Haikou rural commercial bank Limited by Share Ltd Pledgor: Hainan Jingrun Zhenzhu Biological Technology Co., Ltd. Registration number: 2016460000001 |
|
| PLDC | Enforcement, change and cancellation of contracts on pledge of patent right or utility model | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20200831 Granted publication date: 20121003 Pledgee: Haikou rural commercial bank Limited by Share Ltd. Pledgor: HAINAN JINGRUN PEARL BIOTECHNOLOGY Co.,Ltd. Registration number: 2016460000001 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right |