CN102166243B - Artemisia rupestris preparation and application thereof in medicaments for treating diabetes - Google Patents

Abstract

The invention discloses an artemisia rupestris preparation used in medicaments for treating diabetes and a preparation method thereof. The preparation method comprises the following steps: crushing artemisia rupestris herbs; weighing the powder; adding ethyl ether of which the volume is 3 times of the volume of the powder; standing for 8 hours, and then, filtering; ultrasonically extracting precipitates with 80% ethanol at 35 DEG C for 30-40 minutes every time; filtering to obtain an ethanol extracting solution; precipitating with 95% ethanol, centrifuging, and taking a supernatant solution; extracting ethyl acetate; filtering to obtain an ethyl acetate part; carrying out polyamide column chromatography on the ethyl acetate part; eluting by sequentially adopting ethanol solutions with different concentrations of 20%, 40%, 60% and 80%; collecting ethanol eluate obtained by adopting the ethanol solutions with the concentrations of 60% and 80%; concentrating while depressurizing to obtain an extract; proportioning the obtained extract and a milk vetch extract according to the weight ratio of (5-10):1; and preparing a certain amount of pure water to obtain a finished preparation. The prepared artemisia rupestris preparation has the activity of reducing blood sugar, the using dose of the artemisia rupestris preparation is 67.5mg/kg, and the artemisia rupestris preparation has wide application fields in medicaments for treating diabetes.

Description

Xinjiang Yizhi Artemisia preparation and its application in the preparation of medicines for treating diabetes

field of invention

The invention relates to the technical field of Chinese herbal medicine preparations. Specifically, the present invention relates to the technical field of preparation and preparation of Artemisia xinjiang used in medicines for treating diabetes. the

Background technique

In recent years, due to many factors such as the improvement of living standards, changes in dietary structure, increasingly tense pace of life, and less active and more sedentary lifestyle, the incidence of diabetes worldwide has increased rapidly. Diabetes has become the third largest disease after tumors and cardiovascular diseases. Chronic diseases that seriously threaten human health. For the treatment of diabetes, western medicine mostly adopts methods such as diet, oral hypoglycemic drugs, and insulin replacement. Natural medicine has the advantages of rich sources, less toxic and side effects, easy to accept and long-term use. Therefore, in recent years, the research and development of natural hypoglycemic substances in plants at home and abroad is very active. the

Diabetes mellitus (DM, Diabetes Mellitus) is a metabolic disorder syndrome characterized by chronic hyperglycemia associated with genetic factors and various environmental factors. Although there are some drugs with different targets for the treatment of diabetes, sometimes The expected therapeutic effect cannot be achieved. Therefore, to be able to develop highly effective drugs for the treatment of diabetes is the core issue in the treatment of diabetes. In recent years, studies have found that the hypoglycemic effect of traditional Chinese medicine is mild and long-lasting, with few side effects, and the mechanism of action has the characteristics of multi-effect, multi-site and multi-function. Therefore, the development of high-efficiency and low-toxicity anti-diabetic traditional Chinese medicine will be a research field with development prospects, and the medicinal effect of Artemisia Xinjiang has become a research hotspot in recent years. Practical significance. the

Contents of the invention

Aiming at the technical status of the application of Artemisia xinjiang in medicines for treating diabetes that have not been reported in the prior art, the present invention aims to provide Artemisia xinjiang preparations and its application in medicines for treating diabetes. the

Technical scheme of the present invention:

By using Artemisia xinjiang as raw material, the whole herb of Artemisia xinjiang was crushed, soaked in ether solution, filtered and rotary evaporated to remove the ether solution, then extracted with ethanol ultrasound, the extract was precipitated with ethanol, centrifuged, extracted with ethyl acetate, polymerized Amide column chromatography, final concentration to make extract, and the concentrated extract and astragalus extract are dissolved in water according to weight ratio to make liquid preparation. The preparation is used in medicines for treating diabetes, has small dosage, high curative effect, and good hypoglycemic activity after testing. the

Specifically, the present invention provides a preparation of Artemisia xinjiang used in medicines for treating diabetes. The whole herb of Artemisia xinjiang is pulverized, weighed and added with 3 times the volume of ether, and then filtered for 8 hours. The precipitate is mixed with 80% ethanol in Ultrasonic extraction at 35°C, 30-40min each time, filter to obtain the ethanol extract of Artemisia argyi, precipitate with 95% ethanol overnight, centrifuge to take the supernatant, extract with ethyl acetate, the extraction temperature is normal temperature, and filter to obtain the ethyl acetate part , polyamide column chromatography at the ethyl acetate site was sequentially eluted with ethanol solutions of different concentrations of 20%, 40%, 60%, and 80%, and the 60% and 80% ethanol eluates were collected, concentrated under reduced pressure to form an extract, The concentrated extract and Astragalus extract are compatible in a weight ratio (5-10): 1, and a certain amount of pure water is prepared according to the usual practice of Chinese herbal medicine liquid medicaments to obtain a finished preparation. the

Further, the present invention provides a preparation method of Artemisia annua preparations used in medicines for treating diabetes, which specifically includes the following steps:

(1), first crush the whole Artemisia argyi in Xinjiang into powder, weigh and add 3 times the volume of ether and place it for 8 hours before filtering, and the precipitate is ultrasonically extracted with 80% ethanol at 35°C, each time for 30-40min, and filtered to obtain Xinjiang Artemisia argyi ethanol extract;

(2) Precipitate the Artemisia argyi extract with 95% ethanol overnight, filter, remove the ethanol by rotary evaporation, extract with ethyl acetate, the extraction temperature is normal temperature, and obtain the ethyl acetate part, and the ethyl acetate part is subjected to polyamide column chromatography Adopt 20%, 40%, 60%, 80% ethanol solutions of different concentrations to elute successively, collect 60% and 80% ethanol eluents and concentrate them under reduced pressure to form extracts. Ratio (5-10): 1 ratio compatibility, according to the usual practice of Chinese herbal medicine liquid medicine, a certain amount of pure water is prepared to prepare the finished preparation. the

Xinjiang Yizhi Artemisia (Artemisia rupestris L.), also known as Rock Artemisia, Artemisia genus of Asteraceae, is a folk traditional medicine of Kazak and Uyghur in Xinjiang. "Uyghur medicine sub-volume and "Uyghur medicine annals (volume 1)". Xinjiang Yizhi Artemisia is a perennial herb, 20-50cm high, and the whole plant has a specific fragrance. The stem is cylindrical, with a diameter of 1mm to 4mm. There are purple stems and light yellow-green stems. The surface is slightly longitudinally ridged, brittle, and the cross-section is light yellow and hollow. It has a special aroma and a light taste. Leaves green, light yellow when dry, lobes narrowly lanceolate, 2mm-8mm long, 0.5mm-1mm wide, sparsely pubescent on both sides, sessile or short-stalked, with false stipules at the base. Capitulum hemispherical, yellow-purple, diameter 5mm-8mm, stalk length 3mm-5mm, involucre 3-4 rows, outer herbaceous strip. Most of them germinate from late May to early June, and grow slowly. Buds appear in early to mid-July, and they begin to bloom in August. They can open until mid-September. The first batch of flowers that bloom will enter the fruiting period at this time , the fruit period can be extended to the first ten days of October, and soon withers and enters the dormant period. Domestic clinical data show that as a national medicine, it has the functions of clearing heat and detoxifying, invigorating the stomach and eliminating food, calming vomiting, and anti-allergy. It is used to treat snake bites, urticaria, indigestion, colds, hepatitis, and allergic diseases. the

The Radix Astragali extract selected in the present invention can be obtained by common technical means well known in the art. the

The present invention studies the hypoglycemic effect of the prepared Xinjiang Yizhi Artemisia preparation by inducing alloxan to establish a mouse diabetes model, and the optimal dosage is 67.5 mg/kg. the

By implementing the specific content of the invention of the present invention, the following effects can be achieved:

1. The present invention extracts the prepared Artemisia argyi preparation, analyzes the body weight, blood sugar, SOD and MDA changes of alloxan diabetic KM mice after administration, and observes its tissue morphology to explore the extraction of Artemisia argyi. The effect of liquid on diabetic KM mice. The results show that the Xinjiang Yizhi Artemisia preparation provided by the invention has good hypoglycemic activity, and has broad application prospects in the preparation of drugs for treating diabetes. the

2. The reagents required by the present invention are all analytically pure, and the preparation method is ultrasonic extraction technology. The operation is simple and fast, without heating, the extraction rate is high, the speed is fast, the structure of the extract is not destroyed, and the effect is good, showing obvious advantages. It can be directly scaled up for production. the

Description of drawings

Figure 1 shows the flow chart of the preparation process of Xinjiang Yizhi Artemisia preparation. the

Figure 2 shows the effects of Xinjiang Yizhi Artemisia preparation on the liver of experimental hyperglycemia mice. In the figure, a represents the liver of diabetic mice (HE staining, × 10), and b represents the liver treated with Xinjiang Yizhi Artemisia preparation (HE staining, × 10). 10), c represents the liver of drug-treated mice (HE staining, ×10), d represents the liver of normal mice (HE staining, ×10). the

Figure 3 shows the effect of Xinjiang Yizhihao preparation on the kidneys of experimental hyperglycemic mice. In the figure, a represents the kidneys of diabetic mice (HE staining, × 10), and b represents the kidneys treated with Xinjiang Yizhihao preparations (HE staining, × 10). 10), c represents drug-treated mouse kidney (HE staining, ×10), d represents normal mouse kidney (HE staining, ×10). the

Detailed ways

Hereinafter, the present invention will be described with reference to examples, but the present invention is not limited to the following examples. the

The main raw and auxiliary materials and equipment involved in the present invention are:

Main raw materials and selected reagents: Xinjiang Artemisia argyi, collected from a branch of Tianshan Mountain---North Road of Tiangeer Mountain, dried naturally in a cool place after harvesting, crushed and bagged for later use. Radix Astragali extract can be obtained by common technical means well known in the art. the

Kunming white mice, male, were purchased from the Medical Experimental Animal Center of Xinjiang Medical University (animal license number: SYXK (new) 2003-0001). the

Alloxan, purchased from Sigma Company of the United States; SOD, product of Nanjing Jiancheng Bioengineering Research Institute; MDA, product of Nanjing Jiancheng Bioengineering Research Institute: petroleum ether, analytical reagent, Tianjin Fuyu Fine Chemical Co., Ltd.; absolute ethanol, Analytical reagents, Tianjin Fuyu Fine Chemical Co., Ltd.; ethyl acetate, analytical reagents, Tianjin Fuyu Fine Chemical Co., Ltd. Dimethylguanidine hydrochloride was purchased from Beijing Zhonghui Pharmaceutical Co., Ltd. the

Main instrument:

Ultrasonic cell grinder soundproof box, Ningbo Xinzhi Biotechnology Co., Ltd.; JFSD-70 laboratory universal grinder, Shanghai Jiading Grain and Oil Testing Instrument Co., Ltd.; RE-5299 rotary evaporator, Gongyi Yuhua Instrument Co., Ltd. ; Circulating water vacuum pump, Gongyi Yuhua Instrument Co., Ltd.; AL104-IC electronic balance, Mettler-Toledo Instrument Co., Ltd.; KQ-250DE CNC ultrasonic cleaner, Kunshan Ultrasonic Instrument Co., Ltd.; Lekang Whole blood glucose meter, product of Roche Diagnostics (batch number 2005-06 22381501). the

All reagents and instruments selected in the present invention are well-known and selected in the art, but do not limit the implementation of the present invention. Some other reagents and equipment well-known in the art are applicable to the implementation of the following embodiments of the present invention. the

Embodiment one: the preparation of Xinjiang Artemisia chinensis preparation

Referring to accompanying drawing 1, specifically describe the preparation of Xinjiang Yizhi Artemisia preparation as follows:

(1), first crush the whole Artemisia argyi in Xinjiang into powder, weigh and add 3 times the volume of ether and place it for 8 hours before filtering, and the precipitate is ultrasonically extracted with 80% ethanol at 35°C, each time for 30-40min, and filtered to obtain Xinjiang Artemisia argyi ethanol extract;

(2) Precipitate the Artemisia argyi extract with 95% ethanol overnight, filter, remove the ethanol by rotary evaporation, extract with ethyl acetate, the extraction temperature is normal temperature, and obtain the ethyl acetate part, and the ethyl acetate part is subjected to polyamide column chromatography Adopt 20%, 40%, 60%, 80% ethanol solutions of different concentrations to elute successively, collect 60% and 80% ethanol eluents and concentrate them under reduced pressure to form extracts. Ratio (5-10): 1 ratio compatibility, according to the usual practice of Chinese herbal medicine liquid medicine, a certain amount of pure water is prepared to prepare the finished preparation. the

By using the above technical means to obtain Artemisia Xinjiang preparations, analyze the changes in body weight, blood sugar, SOD and MDA of alloxan diabetic KM mice after administration, and observe the tissue morphology, to explore the effect of Artemisia Xinjiang Artemisia extract on diabetic KM mice Rat influence. The results show that the Xinjiang Yizhi Artemisia preparation provided by the invention has good blood sugar-lowering activity by using alloxan to induce and establish a mouse diabetes model, and has broad application prospects in the preparation of drugs for treating diabetes. the

Embodiment two: the preparation of Xinjiang Artemisia chinensis preparation

Referring to accompanying drawing 1, according to the technical scheme described in Example 1, by pulverizing the whole Artemisia xinjiang, adding 3 times the volume of diethyl ether and leaving it for 8 hours to filter, the precipitate was ultrasonically extracted with 80% ethanol at 35°C, Each time for 30-40 minutes, filter to obtain the ethanol extract of Artemisia chinensis, precipitate with 95% ethanol overnight, centrifuge to take the supernatant, extract with ethyl acetate, the extraction temperature is normal temperature, filter to obtain the ethyl acetate part, the ethyl acetate part is poly Amide column chromatography adopts ethanol solutions of different concentrations of 20%, 40%, 60%, and 80% to elute successively, collects 60% and 80% ethanol eluates, concentrates under reduced pressure to form extracts, and concentrates extracts with Astragalus extract is mixed in a weight ratio of 5:1, and a certain amount of pure water is prepared according to the usual practice of Chinese herbal medicine liquid medicine to prepare a finished preparation. the

By analyzing the changes in body weight, blood sugar, SOD and MDA of alloxan diabetic KM mice after administration, and observing their tissue morphology, we explored the effect of Xinjiang Yizhi Artemisia extract on diabetic KM mice. The results show that: in the preparation of Artemisia xinjiang preparation provided by the present invention, the Artemisia xinjiang preparation obtained by adopting the above technical process has significant hypoglycemic activity, and the dosage is 67.5 mg/kg. the

Example 3: Establishment of Experimental Alloxan Diabetic Mouse Model

Get 120 Kunming male mice with a body weight of 18-22 g, and feed them adaptively for one week, randomly select 10 as the normal control group (Nomal control group, NC), and inject all the other 200 mg/kg of alloxan intraperitoneally (alloxan is used for physiological Saline configuration), fasting before injection for 12 hours, resulting in an experimental hyperglycemia model, giving 10% glucose water within 48 hours after the model was built, to prevent hypoglycemic death of animals, after 72 hours, blood was taken from the tail of the mice to measure blood sugar (The mice were fasted for 12 hours before blood collection). Observe the drinking water and feed intake of the animals, and whether the typical "three excesses" symptoms of diabetes appear. Finally, the mice whose blood glucose level was ≥11.1mmol/L were selected as experimental diabetic animal models. the

Alloxan can cause damage to islet β cells, thereby affecting the secretion of insulin and increasing blood sugar in animals. Kunming mice were injected with alloxan to make models, and after 3 days, the symptoms of "three more and one less" of polydipsia, polyphagia, polyuria, and weight loss appeared in each group, and gradually aggravated. After administration, the symptoms of Kunming mice in the high, medium and low dose groups of Xinjiang Yizhi Artemisia preparations were alleviated, and the symptoms were relieved with the progress of the experiment; while the symptoms of the model control group were aggravated with the progress of the experiment. the

Example 4: Hypoglycemic effect of Xinjiang Yizhi Artemisia preparation on experimental hyperglycemic mice

50 diabetic model mice successfully modeled and 10 normal mice were divided into 6 groups, including normal control group, diabetes model control group, drug treatment group and 3 experimental groups:

Diabetic control group (Diabetic control group, DC) was fed with 0.9% normal saline every day;

Xinjiang Yizhi Artemisia preparation low-dose group (Low dose, L), 33.75mg/kg by intragastric administration every day;

Xinjiang Yizhi Artemisia preparation middle dose group (Middle dose, M), 67.5mg/kg orally administered every day;

Xinjiang Yizhi Artemisia preparation high-dose group (High dose, H), 135mg/kg by intragastric administration every day;

The normal control group (Normal control group, NC) was fed with 0.9% normal saline every day;

The drug treatment group (Cure group, CG) was given 1.0g/kg orally every day. the

After continuous administration for 14 days, the body weight was measured once a week, and blood was taken from the tail vein to measure the fasting blood glucose concentration once a week. See Table 1 and Table 2. the

Table 1: Changes in body weight (g) of mice during the experiment

Note: ##p<0.01, #p<0.05 compared with normal control group **p<0.01, *p<0.05 compared with model control group

Preliminary experiments proved that the weight of the mice decreased significantly after the injection of alloxan, and there was no significant difference in the weight of the mice 72 hours after the injection of alloxan. As the experiment progressed, the weight changes of the diabetic mice in the three experimental groups were significantly different from those of the diabetic model group (t test, P<0.05), the weight of the mice in the model control group increased slowly, and the animals in the other groups The body weight all increased steadily, indicating that Xinjiang Yizhi Artemisia preparation can improve the weight loss of mice caused by alloxan chemical damage. the

Table 2: Effects of Xinjiang Yizhihao preparations on blood glucose (mmo l/L) levels in diabetic mice

Note: ##p p＜0.01, #p＜0.05 compared with normal control group

**p p＜0.01, *p＜0.05 compared with model control group

From the data in Table 2, it can be seen that the blood sugar level of the model group was significantly higher than that of the normal group, indicating that the experimental modeling was successful. There are significant differences in the hypoglycemic effect of different doses of Xinjiang Yizhi Artemisia preparations on diabetic mice (p<0.01). The high-dose group and the middle-dose group of Yizhihao preparation can reduce the hyperglycemia of diabetic KM mice, while the low-dose Yizhihao preparation group has no significant difference compared with the model group (p>0.05), and the difference compared with the normal control group Extremely significant (p<0.01), indicating that the low-dose Xinjiang Yizhihao preparation group has no obvious hypoglycemic effect. When the dose was 67.5 mg/kg, the effect of lowering blood sugar was the strongest (P<0.01), and the blood sugar was not significantly lowered if the dose was continued to increase. In this experiment, 67.5mg/kg was selected as the optimal dose for the treatment of diabetes. the

Example 5: Effect of Xinjiang Yizhi Artemisia preparation on SOD and MDA enzyme activities in experimental hyperglycemic mice

Fasting for 12 hours before the last administration, blood was collected from the orbital vein 2 hours after the administration, and the serum was separated by centrifugation at 6000r/min for 15 minutes within 0.5 hours, and the fasting serum superoxide dismutase (SOD) and (MDA) indexes were determined according to the kit method. the

(1) Determination of serum SOD activity

Determination method: take serum, use superoxide dismutase assay kit for the determination of superoxide dismutase, and operate according to the instructions. the

Formula: total SOD activity (U/ml) = [(absorbance of control tube-absorbance of measuring tube)/absorbance of control tube absorbance]/50%*dilution multiple of reaction system*dilution multiple of sample before test

Steps

Note: a * represents sample sampling volume and distilled water sampling volume

Mix well, place at room temperature for 10 min, and measure SOD activity in serum in a microplate reader. The results are shown in Table 3. the

(2) Determination of serum MDA content

Assay method: take serum, and use a lipid peroxide assay kit for the determination of lipid peroxide, and operate according to the instructions. the

Steps

the Standard tube Standard Blank Tube measuring tube Assay blank tube** 10nmol/ml standard (ml) a* - - the Absolute ethanol (ml) - a* - the Test sample (ml) - - a* the Mixed reagent (ml) 4 4 4 the

Note: a* means that the required sample volume, standard product volume, absolute ethanol volume, and reagent 1 volume are all equal. the

**Indicates that the blank tube for determination can be omitted and replaced by a standard blank tube. the

Vortex mixer to mix evenly, tie the mouth of the test tube tightly with plastic wrap, prick a small hole with a needle, bathe in 95°C water for 40 minutes, take it out and cool it with running water, then centrifuge at 3500-4000r/min for 10min (centrifugation below 3000r/min needs to be prolonged) , the purpose is to make the precipitation complete) to get the supernatant, and use a microplate reader to measure its activity in the microplate plate. the

Formula: MDA content in serum (nmol/ml) = (absorbance of measuring tube - absorbance of measuring blank tube) / (absorbance of standard tube - absorbance of standard blank tube) * standard concentration (10nmol/ml) * dilution factor of sample before testing

Table 3: Effect of Xinjiang Yizhi Artemisia preparation on SOD and MDA in diabetic mice

Note: ##p<0.01, #p<0.05 compared with normal control group **p<0.01, *p<0.05 compared with model control group

SOD plays an important role in the oxidation and anti-oxidation balance of the body. It can remove superoxide anion free radicals and protect cells from damage. MDA is a lipid peroxidation product produced by unsaturated fatty acids in the body under the action of free radicals. It can be seen from Table 3 that after the action of Alloxan on the islet cells, the SOD activity decreased, while the MDA increased significantly (p<0.05), indicating that the islet cells were damaged to a certain extent. After giving Xinjiang Yizhihao preparation, its SOD value increased significantly, and the three dosage groups of Yizhihao preparation (high, medium and low) had significant differences compared with the model group (p<0.05), and the content of MDA was significantly higher than that of the model group. Compared with the model group, there were significant differences (p<0.05) between the three dosage groups and the model group, which indicated that the antioxidant capacity of Artemisia chinensis preparation was also improved. the

Example 6: Effects of Xinjiang Yizhi Artemisia preparation on the liver and kidney of experimental hyperglycemic mice

The mice were killed by cervical dislocation, and the liver and kidney tissues were dissected immediately to make paraffin sections:

The samples were fixed with 10% formaldehyde solution, dehydrated with different concentrations of ethanol, embedded in paraffin, sectioned with a microtome, stained with HE, observed and photographed. Observation by microscope showed that:

See a, b, c, d in accompanying drawing 2. In the normal group, the structure of the hepatic lobule was normal, no fibrous hyperplasia and formation of fibrous septa were observed, the ratio of hepatic cord and sinus was normal, and there was no inflammatory cell infiltration; , oval or irregular hepatocyte nodules (pseudolobules), the hepatocyte cords in the nodules are arranged in disorder, the hepatocyte nuclei are enlarged, and the staining is deepened; Most of the mouse hepatic lobules had clear structures, and a small amount of structures were blurred. The arrangement of hepatic cell cords was basically clear, and some cells were swollen. It shows that the Artemisia xinjiang preparation provided by the invention has a good hepatoprotective effect. the

See a, b, c, d in accompanying drawing 3. After the mice were sacrificed, their kidneys were dissected and taken out to find that the kidneys of the mice in the control group were swollen, the glomeruli were diffusely enlarged, and the capillary cavity was narrowed; the capillaries of the mice in the normal group were normal; , High-dose group mice kidney injury status has a certain improvement. It shows that the Xinjiang Yizhi Artemisia preparation provided by the invention has a good improvement effect on kidney damage. the

Claims (3)

1. An Artemisia sylvestris preparation applied in medicine for treating diabetes is characterized in that, by pulverizing the whole herb of Artemisia sylvestris Xinjiang, weighing and adding 3 times the volume of diethyl ether is placed for 8h and then filtered, and the precipitate is filtered with 80% ethanol at 35 Ultrasonic extraction at ℃, 30-40min each time, filter to obtain the ethanol extract of Artemisia argyi, precipitate with 95% ethanol overnight, centrifuge to take the supernatant, extract with ethyl acetate, the extraction temperature is normal temperature, filter to obtain the ethyl acetate part, Ethyl acetate part polyamide column chromatography was eluted with 20%, 40%, 60%, and 80% ethanol solutions in sequence, and 60% and 80% ethanol eluates were collected, concentrated under reduced pressure to form extracts, and concentrated The final extract and Astragalus extract are compatible in a weight ratio (5-10): 1 ratio, and according to the usual practice of Chinese herbal medicine liquid medicaments, a certain amount of pure water is prepared to obtain a finished preparation; obtained by technical means. 2. a preparation method of Xinjiang Artemisia chinensis preparation as claimed in claim 1, is characterized in that, specifically comprises the steps: (1), first, crush the whole Artemisia argyi in Xinjiang into powder, weigh and add 3 times the volume of ether, place it for 8 hours, filter it, and extract the precipitate with 80% ethanol ultrasonically at 35°C, each time for 30-40min, and filter to obtain Artemisia argyi ethanol extract; (2) Precipitate the Artemisia argyi extract with 95% ethanol overnight, filter, remove the ethanol by rotary evaporation, extract with ethyl acetate, the extraction temperature is normal temperature, and obtain the ethyl acetate part, and the ethyl acetate part is subjected to polyamide column chromatography Adopt 20%, 40%, 60%, 80% ethanol solutions of different concentrations to elute successively, collect 60% and 80% ethanol eluate and concentrate under reduced pressure to form extractum, extractum after concentration and Radix Astragali extract by weight Ratio (5-10): 1 ratio compatibility, according to the common practice of Chinese herbal medicine liquid medicine, a certain amount of pure water is prepared to prepare the finished preparation; the astragalus extract is obtained by conventional technical means in the field. 3. The application of Xinjiang Yizhi Artemisia preparation in the preparation of medicine for treating diabetes as claimed in claim 1, characterized in that, the dosage is 67.5mg/kg. the

Images