CN102033307A

CN102033307A - Dew prevention device for low-temperature microscope stage

Info

Publication number: CN102033307A
Application number: CN 201010508045
Authority: CN
Inventors: 李巧巧; 陈儿同; 杨晓芸; 张华�; 徐文强
Original assignee: University of Shanghai for Science and Technology
Current assignee: University of Shanghai for Science and Technology
Priority date: 2010-10-15
Filing date: 2010-10-15
Publication date: 2011-04-27
Anticipated expiration: 2030-10-15
Also published as: CN102033307B

Abstract

The invention relates to a low-temperature microscope, in particular to a dew prevention device for a low-temperature microscope stage, comprising a nitrogen bottle, a pressure reducing valve, a drying device, a flowmeter, a liquid nitrogen bottle, a temperature adjusting chamber, a low-temperature stage and a rewarming chamber, wherein the liquid nitrogen bottle is internally provided with a heat exchanger, the nitrogen bottle is connected with the liquid nitrogen bottle internally provided with the heat exchanger through an outlet pipeline, the pipeline is sequentially provided with the pressure reducing valve, the drying device and the flowmeter, the liquid nitrogen bottle internally provided with the heat exchanger is connected to a low-temperature chamber of the low-temperature stage of the inverted low-temperature microscope through a pipeline with the temperature adjusting chamber, the rewarming chamber is connected to a nitrogen outlet pipeline of the low-temperature stage, an objective lens of the inverted low-temperature microscope is arranged below the low-temperature stage, and a spotlight is arranged on the low-temperature stage. The invention mainly solves the technical problem of easy dewing of a stage glass sheet of the low-temperature microscope stage in use, has the advantages of compact and small structure and convenience for use and can be configured both in a large laboratory and a small laboratory.

Description

A kind of anti-condensation device that is used for the cryomicroscope objective table

Technical field

The present invention relates to cryomicroscope, particularly a kind of anti-condensation device that is used for the cryomicroscope objective table.

Background technology

Cryomicroscope is widely used in the low-temperature biological medical domain, mainly is the situation of change of passing through various cells of cryo-microscope sem observation and biological tissue under low temperature environment.Now, people cross the formation of the inside and outside ice crystal of cell, the variation of cell appearance size etc. with the cryomicroscope Direct observation, and a large amount of important information materials have been obtained, thereby can qualitative, quantitative ground analysis and research low temperature to the influence of subjects, greatly promoted the development of low-temperature biological medical domain, these achievements obtain the importance that makes people further recognize cryomicroscope.

The cryomicroscope of experience development decades, though brought a lot of gratifying achievements, cryomicroscope still exists deficiency, the anti-condensation problem of low temperature objective table particularly, in case under observation the glass sheet Mechanism of External Condensation of Vapor has influenced passing through of visible light, observation will not gone down.Previous design is that this texture ratio is heavy, and takes up space very much, uses also very inconvenience with whole cover such as spotlight, low temperature objective table and object lens, and cost is than higher, and causes the waste of nitrogen.Some semi-enclosed objective table structures appear again in the later stage, be that the nitrogen that objective table is discharged is covered the glassy layer surface, but the nitrogen that temperature is lower can make airborne water vapor be liquefied as the globule to be dripped on glassly, can influence light path equally and pass through, and the outlet of cold nitrogen can produce frosting.

Summary of the invention

The object of the present invention is to provide a kind of anti-condensation device that is used for the cryomicroscope objective table, mainly solve existing cryomicroscope objective table in use, the technical matters of the easy dewfall of objective table glass sheet, its compact conformation is small and exquisite, easy to operate, big or small laboratory is all configurable.

The technical scheme that the present invention addresses the above problem is.

A kind of anti-condensation device that is used for the cryomicroscope objective table is characterized in that: it comprises that nitrogen cylinder, reduction valve, drying device, flowmeter, inside have the liquid nitrogen bottle of heat interchanger, tempering room, low temperature objective table and rewarming chamber; Described nitrogen cylinder is connected to the liquid nitrogen bottle that inside has heat interchanger by discharge pipe, has reduction valve, drying device, flowmeter on the pipeline successively; The liquid nitrogen bottle that described inside has a heat interchanger is connected in the low temperature cavity of low temperature objective table of inversion type cryomicroscope by the pipeline with tempering room again, is connected with the rewarming chamber on the nitrogen outlet pipeline of described low temperature objective table; The object lens of described inversion type cryomicroscope are positioned under the low temperature objective table, and spotlight is positioned on the low temperature objective table.

The low temperature cavity of described low temperature objective table is arranged at up and down between the view window, described view window up and down constitutes by triplex glass, being exactly the low temperature cavity that directly contacts with the low temperature nitrogen of importing from draft tube between the inner layer glass, is the space of circulation through rewarming nitrogen between glass outer and the middle level glass; The nitrogen outlet pipeline of low temperature objective table is communicated to the space between glass outer and the middle level glass after the rewarming that passes through the rewarming chamber with heater strip is handled.

Beneficial effect of the present invention is: 1. the nitrogen of low temperature cavity discharge is through being heated to more than the water vapor dewpoint temperature, between skin and middle level glass, discharge then, both discharged the air in the glassy layer, the side Yin Wendu that avoids glass outer to contact with air again is lower than dewpoint temperature and the dewfall frosting is used very reliable.2. compare with the device that spotlight, objective table and object lens are all covered, this objective table nitrogen consumption significantly reduces, and saves a large amount of nitrogen.3. compact conformation is small and exquisite, and is easy to operate, and big or small laboratory is all configurable.

Description of drawings

Fig. 1 is an one-piece construction synoptic diagram of the present invention.

Fig. 2 is the partial structurtes synoptic diagram of low temperature objective table annex among the present invention.

Embodiment

See also Fig. 1, the invention discloses a kind of anti-condensation device that is used for the cryomicroscope objective table.As shown in the figure: it comprises that nitrogen cylinder 1, reduction valve 2, drying device 3, flowmeter 4, inside have the liquid nitrogen bottle 6 of heat interchanger 5, tempering room 7, low temperature objective table 11 and rewarming chamber 10; Described nitrogen cylinder 1 is connected to the liquid nitrogen bottle 6 that inside has heat interchanger 5 by discharge pipe, has reduction valve 2, drying device 3, flowmeter 4 on the pipeline successively; The liquid nitrogen bottle 6 that described inside has a heat interchanger 5 is connected in the low temperature cavity of low temperature objective table 11 of inversion type cryomicroscope by the pipeline with tempering room 7 again, is connected with rewarming chamber 10 on the nitrogen outlet pipeline 9 of described low temperature objective table 11; The object lens 12 of described inversion type cryomicroscope are positioned at the low temperature objective table 11 times, and spotlight 8 is positioned on the low temperature objective table 11.

During use, the nitrogen that comes out from nitrogen cylinder 1 is the heat interchanger 5 through reduction valve 2, drying device 3, flowmeter 4, liquid nitrogen bottle 6, tempering room 7, low temperature objective table 11 and rewarming chamber 10 successively, flow out from pipeline 9, skin and middle the passing of middle level glass from low temperature objective table 11 view windows enters atmosphere at last.The used microscope of this covering device is the inversion type cryomicroscope, and spotlight 8 and object lens 12 belong to the part of cryomicroscope.The water vapor that mixes in the nitrogen cylinder 1 is flowed through heat interchanger 5 owing to the temperature reduction causes dewfall, for the moisture that prevents dewfall enters low temperature platform 11, make glass sheet smudgy, we have added drying device 3 between reduction valve 2 and flowmeter 4, because the object lens 12 of inversion type cryomicroscope are positioned at the low temperature objective table 11 times, so the sample on the microslide is positioned on the bottom glass sheet of low temperature cavity.

See also Fig. 2 again, apparatus of the present invention further comprise following structure: the low temperature cavity 111 of described low temperature objective table 11 is arranged at up and down between the view window, described view window up and down constitutes by triplex glass, being exactly the low temperature cavity 111 that directly contacts with the low temperature nitrogen of importing from draft tube 110 between the inner layer glass 112, is the space of circulation through rewarming nitrogen between glass outer 114 and the middle level glass 113; The nitrogen outlet pipeline 9 of low temperature objective table 11 is communicated to the space between glass outer 114 and the middle level glass 113 after the rewarming that passes through the rewarming chamber 10 with heater strip 101 is handled.

During use, low temperature nitrogen enters the low temperature cavity 111 of low temperature objective table 11 from draft tube 110, in low temperature cavity 111, cool off sample, enter then in the rewarming chamber 10, through the heating of electrical heating wire 101, low temperature nitrogen heat absorption rewarming is to more than the water vapor dewpoint temperature, enter in the space that surrounds between skin and the middle level glass along nitrogen outlet pipeline 9 (can be set to two-way), at last, useless nitrogen is discharged from exhausr port, thereby avoids dewfall, the frosting of glass outer upper and lower surface.

In sum, be preferred embodiment of the present invention only, be not to be used for limiting practical range of the present invention, promptly all equivalences of doing according to the content of the present patent application claim change and modify, and all should be technology category of the present invention.

Claims

1. anti-condensation device that is used for the cryomicroscope objective table is characterized in that: it comprises that nitrogen cylinder (1), reduction valve (2), drying device (3), flowmeter (4), inside have the liquid nitrogen bottle (6) of heat interchanger (5), tempering room (7), low temperature objective table (11) and rewarming chamber (10); Described nitrogen cylinder (1) is connected to the liquid nitrogen bottle (6) that inside has heat interchanger (5) by discharge pipe, has reduction valve (2), drying device (3), flowmeter (4) on the pipeline successively; The liquid nitrogen bottle (6) that described inside has heat interchanger (5) is connected in the low temperature cavity of low temperature objective table (11) of inversion type cryomicroscope by the pipeline with tempering room (7) again, is connected with rewarming chamber (10) on the nitrogen outlet pipeline (9) of described low temperature objective table (11); The object lens of described inversion type cryomicroscope (12) are positioned under the low temperature objective table (11), and spotlight (8) is positioned on the low temperature objective table (11).

2. the anti-condensation device that is used for the cryomicroscope objective table according to claim 1, it is characterized in that: the low temperature cavity (111) of described low temperature objective table (11) is arranged at up and down between the view window, described view window up and down constitutes by triplex glass, being exactly the low temperature cavity (111) that directly contacts with the low temperature nitrogen of importing from draft tube (110) between the inner layer glass (112), is the space of circulation through rewarming nitrogen between glass outer (114) and the middle level glass (113); The nitrogen outlet pipeline (9) of low temperature objective table (11) is communicated to the space between glass outer (114) and the middle level glass (113) after the rewarming that passes through the rewarming chamber (10) with heating (101) is handled.