CN201060192Y - A Composite Testing System for Low Temperature Microscopy and Differential Scanning Calorimetry - Google Patents

Abstract

A low-temperature microscopic and differential scanning calorimetry composite test system, which includes an inverted optical microscope, a low-temperature microscopic and differential scanning calorimetric composite device, cold supply equipment, computer data acquisition system, temperature controller and camera Video system; its features are: the composite device of cryogenic microscopy and differential scanning calorimetry is located in the plexiglass cover, and is fixed on the microscope sample stage support with screws; the top of the plexiglass cover is connected to the microscope condenser, and the bottom is connected to the objective lens , the objective lens is connected to the microscope objective lens seat, and the microscope objective lens seat and the microscope column are connected to the microscope base; the heater and temperature sensor in the low temperature microscopy and differential scanning calorimetry composite device are connected to the temperature controller, and the temperature controller is connected to the temperature controller in turn. The computer is connected; the objective lens is connected with a camera, a video camera, and a television monitor. The utility model can obtain the microscopic image and various physical and chemical parameters of the sample in the process of heating and cooling at the same time, and the test result is more accurate.

Description

A Composite Testing System for Low Temperature Microscopy and Differential Scanning Calorimetry

technical field

The utility model relates to a low temperature microscope and differential scanning calorimetry composite test system.

Background technique

Low temperature biological microscopy and differential scanning calorimetry (DSC) are two very important tools in low temperature biology and food research. Low-temperature biological microscope can directly observe and record the morphological changes of organisms in the process of heating and cooling; while DSC can obtain physical and chemical data in this process. The real-time morphological and physicochemical data analysis of organisms is very important for cryobiology, but the usual DSC test and cryomicroscope test are done separately, although the test parameters can be set to the same value, but because they are not "the same event ", unable to unify the results in time and space; especially with the development of food and biomaterial science and technology, vitrification is a new low-temperature preservation technology that has emerged in the past ten years. One of the prerequisites for achieving vitrification preservation is to accurately measure the glass transition temperature of the material. However, due to the complexity of food and biological materials, the single DSC measurement method is less sensitive than the thermomechanical method, and it will appear on the DSC curve. The steps of the glass transition are small, and it is not easy to judge the glass transition point; sometimes several parts similar to the glass transition may appear on the DSC curve of some samples, which is easy to cause misjudgment. From the perspective of the development process of cryobiology, It has reached the point where a comprehensive study must be conducted using multiple means.

Utility model content

In order to overcome the shortcomings in the prior art that the DSC test and the low-temperature microscope test are performed separately, the results cannot be unified in time and space, and the test results have low precision and are likely to cause misjudgment. The present invention proposes a low-temperature A new low-temperature micro-DSC system combining microscopy techniques and differential scanning calorimetry. The present invention does not simply put cryogenic microscope and DSC together, but integrates the respective characteristics of large cryogenic biological microscope and differential scanning calorimeter, and develops a new type of low-temperature micro-DSC system, which enables the observation of samples At the same time as the morphological change, its physical and chemical data information can be obtained. Combining the two for comparison and analysis can obtain more accurate results.

Concrete technical scheme of the present utility model is realized like this:

A low temperature microscopic and differential scanning calorimetry composite test system, which includes an inverted optical microscope, a low temperature microscopic and differential scanning calorimetric composite device, cold supply equipment, computer data acquisition system, temperature controller and camera Video system. Inverted optical microscope consists of microscope condenser, microscope sample stage bracket, microscope column, microscope condenser bracket, objective lens, microscope objective lens seat, microscope base; computer data acquisition and control system consists of computer, monitor, printer and temperature controller Composition; video recording system is composed of camera, video camera, video recorder and TV display; its characteristics are: low temperature microscopy and differential scanning calorimetry composite device is located in the plexiglass cover, and fixed on the microscope sample stage support with screws; plexiglass The top of the cover is connected to the microscope condenser, the bottom is connected to the objective lens, the objective lens is connected to the microscope objective lens base, the microscope objective lens base and the microscope column are connected to the microscope base; the heater and The temperature sensor is connected with the temperature controller, and the temperature controller is connected with the computer in turn; the objective lens is connected with the camera, the video camera, and the television monitor.

The cold supply equipment is a liquid nitrogen pressure vessel; the cryogenic microscopy and differential scanning calorimetry composite device adopts liquid nitrogen refrigeration.

The microscope sample stage support of the cryogenic microscopy and differential scanning calorimetry composite device is made of light-transmitting materials.

The composite device of cryogenic microscopy and differential scanning calorimetry can control the heating and cooling rate of the sample.

The utility model combines the low-temperature microscopic technology and the differential scanning calorimetry technology, and can obtain the microscopic image and various physical and chemical parameters of the sample during the heating and cooling process at the same time, and combine the two for analysis, mutual comparison and confirmation , can get more accurate results; the utility model also has the advantages of simple structure, convenient use, high test precision, modern test means and the like.

Description of drawings

Below in conjunction with accompanying drawing and embodiment the utility model is described further.

Fig. 1 is a schematic diagram of the overall structure of the utility model.

Fig. 2 is a side view of the main structure of the utility model

1. Liquid nitrogen pressure vessel, 2. Screws, 3. Microscope sample stage bracket, 4. Microscope column, 5. Microscope condenser bracket, 6. Microscope condenser, 7. Organic glass cover, 8. Low temperature microscope and Differential scanning calorimetry compound device, 9. Objective lens, 10. Cold nitrogen outlet hose, 11. Microscope objective lens holder, 12. Microscope base, 13. Printer, 14. Computer, 15. Monitor, 16. Temperature controller, 17 .camera, 18. video camera, 19. video recorder, 20. television monitor.

Detailed ways

The cryogenic microscopy and differential scanning calorimetry compound device 8 is located in the plexiglass cover 7, and is fixed on the microscope sample stage support 3 with screws 2, and the plexiglass cover 7 is filled with nitrogen gas, which can prevent the low temperature microscopy and differential scanning calorimetry Frosting of composite device 8; the top of cryogenic microscopy and differential scanning calorimetry composite device 8 is microscope condenser 6, which provides light source for microscope, and microscope condenser 6 is fixed on microscope column 4 through microscope condenser bracket 5 Above; below the low temperature microscopy and differential scanning calorimetry composite device 8 is the objective lens 9, which is connected to the microscope objective lens base 11; all the above devices are supported by the microscope base 12. Microscope objective lens seat 11 is connected with camera 17 and video camera 18, can take pictures and videotape to the sample in the cryogenic freezing process, video camera 18 is connected with video recorder 19 and TV display 20 again, can record the change process of sample at low temperature, can watch TV simultaneously directly displayed on the display 20. The heater and temperature sensor in the low temperature microscopy and differential scanning calorimetry composite device 8 are connected to a temperature controller 16 , which is connected to a computer 14 in turn; the computer 14 is connected to a printer 13 and a display 15 . After the whole system is ready and all the power is turned on, liquid nitrogen is supplied to the cryogenic microscopy and differential scanning calorimetry composite device 8 through the liquid nitrogen pressure vessel 1, and the microscope sample stage support cools down after exchanging heat with liquid nitrogen, and cools the sample support and reference support; sample support and reference support are made of transparent quartz glass, have light transmittance; the nitrogen after heat exchange is discharged in the environment from cold nitrogen outlet flexible pipe 10; Then set the liter, the sample by computer 14, The cooling rate is transmitted to the temperature controller 16, and the temperature controller 16 measures the temperature changes of the sample holder and the reference holder through the temperature sensor and compares them, and then outputs current to the heater for heating, so that the sample meets the set liter, cooling rate. The heat flow-temperature relationship data of the whole process is stored in the computer 14 as a data file, displayed on the display 15 in real time, and can be printed out from the printer 13 . In this way, the morphological changes and DSC curves of the same experimental sample under the same experimental conditions can be recorded at the same time, which is of great significance to the study of cryopreservation of biological materials. The utility model can obtain the microscopic image and various physical and chemical parameters of the sample in the process of heating and cooling at the same time.

Claims (3)

1. a cryo-microscope scans calorimetric composite test system with the difference formula, and it includes the inversion type optical microscope, cryo-microscope and difference formula scanning amount heat composite apparatus, cold supply arrangement, computer data acquisition system, temperature controller and pick-up and picture recording system;

The inversion type optical microscope is made of microscope condenser, microscope example bench support, microscope column, microscope condenser support, object lens, micro objective seat, Microscope base; Computer data acquiring and control system are made of computing machine, display, printer and temperature controller; Pick-up and picture recording system is made of camera, video camera, video recorder and television indicator; It is characterized in that: cryo-microscope is positioned at plexiglass tent with difference formula scanning amount heat composite apparatus, and is screwed on the microscope example bench support; The plexiglass tent top is connected with microscope condenser, and the bottom is connected with object lens, and object lens are connected with the micro objective seat, and micro objective seat and microscope column all are connected on the Microscope base; The cryo-microscope well heater interior with differing from formula scanning amount heat composite apparatus is connected with temperature controller with temperature sensor, and temperature controller is connected with computing machine successively; Object lens are connected with camera, video camera, television indicator.

2. a kind of cryo-microscope according to claim 1 and difference formula scanning calorimetric composite test system, it is characterized in that: the cold supply arrangement is a liquid nitrogen pressure vessel, cryo-microscope adopts liquid nitrogen refrigerating with difference formula scanning amount heat composite apparatus.

3. a kind of cryo-microscope according to claim 1 and difference formula scanning calorimetric composite test system, it is characterized in that: cryo-microscope is made by light transmissive material with the microscope example bench support of difference formula scanning amount heat composite apparatus.

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