CN102028680B - Application of fisetin in preparation of anti-eimeria tenella drugs - Google Patents
Application of fisetin in preparation of anti-eimeria tenella drugs Download PDFInfo
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- CN102028680B CN102028680B CN2010105625379A CN201010562537A CN102028680B CN 102028680 B CN102028680 B CN 102028680B CN 2010105625379 A CN2010105625379 A CN 2010105625379A CN 201010562537 A CN201010562537 A CN 201010562537A CN 102028680 B CN102028680 B CN 102028680B
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Abstract
The invention discloses application of fisetin in preparation of an Eimeria tenella resistant medicament. The fisetin is uniformly mixed with acceptable auxiliary materials, and is added into water or feed to effectively resist against Eimeria tenella.
Description
Technical field
The present invention relates to a kind of new purposes of fisetin, the particularly application of fisetin in the preparation medicament for resisting Eimeria tenella.
Background technology
Chicken coccidiosis is the main eqpidemic disease that serious harm intensification poultry husbandry is produced, and the therefore annual and economic loss that causes is up to more than 2,500,000,000 pounds, its cause of disease be the former insects of the multiple door in top Eimeria (
Eimeriaspp), wherein harm is maximum be Eimeria tenella (
Eimeria tenella).Though China does not have accurately statistics so far to this, its loss of the most conservative estimation should be not less than 2,500,000,000 yuan of RMB.Chemotherapy is one of important measures of control chicken coccidiosis, but the chicken coccidiosis has extensive and serious Drug resistance at present, presses for new coccidiostat and appears on the market.
Fisetin (Fisetin), chemical name, 3,3 ＇, 4 ＇, the 7-kaempferol is claimed fisetin again; Butin; Fisetin etc.Can be from the cured tree Rhus succedanea L of Anacardiaceae plant wood.Extract in the plants such as timber.The molecular formula of this chemical compound is C15H10O6, molecular weight 286.23, and CAS accession number: 528-48-3, its chemical constitution is shown in formula I.
The main pharmacological of existing reported in literature has:
1, suppress prostaglandin: the generation to by the fibrocellular prostate rope of the 3T3 mice of methyl cholanthrene transformation has inhibitory action, and half effective inhibition concentration (ID50) is 42.0UM.
2, enzyme suppresses: the complete sugared reductase to the rat crystalline lens has inhibitory action, and half effective inhibition concentration is 10-6M
3, spasmolytic: to the mouse small intestine BIAO and BEN, its effectiveness that causes the convulsion effect to anti-acetylcholine is 166% of papaverine.
4, a group by Japan, American Studies composition of personnel finds that through zoopery the fisetin in vegetable and the fruit helps improving memory
In addition, also have the report of fisetin as biological dye.
Up to now, there is not fisetin to have the relevant report of resisting Eimeria tenella effect, can't infers through the known character of fisetin whether it has the effect of resisting Eimeria tenella.
Summary of the invention
The object of the present invention is to provide the application of fisetin in the preparation medicament for resisting Eimeria tenella.
Fisetin and acceptable auxiliary are processed preparation, be added in feedstuff or the water, by daily method feeding chickling.Zoogenetic infection experiment confirm fisetin has good resisting Eimeria tenella effect, and its anticoccidial index (ACI) can reach more than 150.
The specific embodiment
The anti-chicken coccidiosis experiment of fisetin
Experiment material
Control drug: conventional coccidiostat three word coccidiosis powder (30% sulfaclozine sodium, lot number 001010), produce by Shanghai Novartis animal health company limited;
Fisetin: be mixed with the 2g fisetin in 100 kilograms of feedstuffs, mixing, perhaps dissolving 0.75g fisetin in 100kg water shakes up;
Coccidian oocyst: Eimeria tenella (
Eimeria tenella) Guangdong strain spore egg capsule, preserve rejuvenation in non-ball worm chickling body before using by the Guangdong Provincial Academy Of Agricultural Sciences Veterinary Institute;
Chickling: the yellow chickling in the south of the Five Ridges, provide by Animal Husbandry Inst., Guangdong Prov. Academy of Agricultural Sciences, raise in the special-purpose Animal House of disinfectant; Chicken coop and the equal strict sterilization of used vessel, free choice feeding and drinking pure water; Observe chickling before the test and have or not clinical symptoms and continuous 2d inspection feces to have or not coccidian oocyst, subsequent use.
Feedstuff: by the Xin Nan of Guangdong Province feed corporation,Ltd customization material of brooding all, do not contain any coccidiostat.
Experimental technique
Divide into groups:Press test grouping and processing with 150 south of the Five Ridges Huang breeding chickling to 10 ages in days, pursue and only weigh, young bird and the excessive person of body weight a little less than eliminating will remain chickling and be divided into 5 groups at random, and 30 every group, and suitable the adjustment makes every group of chickling TBW about equally;
Handle:Except that the 1st (blank) group, all the other are respectively organized the equal per os of chickling and irritate clothes Eimeria tenella spore egg capsule l * 10
5Individual/plumage; Except that I group and II (positive control) group, all the other are respectively organized chickling and all after infection, feed immediately or drink feedstuff or the water that contains coccidiostat.
Observation every day and record chicken crowd's mental status, feed intake, feces situation etc.; Dead chickling is weighed, cuts open inspection, if Eimeria tenella infect cause death then lesion score be+4 minutes; All chickling were cutd open inspection by only weighing in the 7th day in the infection back, carried out caecum lesion and kept the score; Calculate the weightening finish and the price of deed of respectively organizing chickling at last.The grouping situation of test chickling is seen table 1.
Drug effect decision method and standard anticoccidial index (ACI) are pressed the formula calculating that Merck company is recommended: ACI=(the relative weight gain rate+survival rate) (egg capsule value+pathological changes value); The relative weight gain rate=(weightening finish of test group weightening finish ÷ blank group) * 100%; Survival rate=(each group survival chickling is counted ÷ and respectively organizes the chickling sum) * 100%.Pathological changes is kept the score by Pyatyi: 1. do not have egg capsule, caecum is normal, is 0 minute; 2. egg capsule is arranged, and the caecum mucosa thickens slightly, has to be dispersed in hemorrhage or a small amount of blood sample intestinal contents on a small quantity, is+1 fen; 3. egg capsule is arranged, and the caecum mucosa thickens, and obviously hemorrhage or obvious blood sample intestinal contents is arranged, and is+2 fens; 4. egg capsule is arranged, and the caecum mucosa thickens, and a large amount of sludged bloods or blood sample intestinal core are arranged, and is+3 fens; 5. chickling is dead or have a large amount of egg capsules, caecum outward appearance to be color of soy sauce (or small intestinal middle part the point-like necrosis region is arranged, the mucosa face is blush) because of coccidiosis, the obvious enlargement of intestinal tube, and content forms tangible blood sample intestinal core, is+4 fens.Pathological changes value in the group=every group of average lesion score * 20.Drug effect criterion is that < 120 for invalid, and 120-160 is weak effect, and 160-180 is middle effect, is strong the effect more than 180 for ACI.The egg capsule value restrains egg sac number (OPG) by cecal content and converts.
Experimental result:
Clinicing symptom observation
Chickling in the matched group of inoculation Eimeria tenella spore egg capsule, it is depressed spirit to occur at the 2nd day, slow-witted upright, loss of appetite; Occurred obvious hemafecia on the 5th day, and death occurs, hemafecia reduces gradually subsequently.
Calculate the anticoccidial index of each group respectively, the result sees shown in the table 2.
Example 1
Powder: get 15g fisetin (content is not less than 99%) and 80g starch mixing, get final product.
The usage of powder: said powder is admixed feedstuff, make to be mixed with the said fisetin of 1.5 grams in per 100 kilograms of feedstuffs, then by daily forage volume feeding.
Example 2
Suspending agent: get 15g fisetin (content is not less than 99%) and add the ethanol stirring, be dissolved in 100 milliliters surely and get final product.
The suspending agent usage: described suspending agent is mixed in drinking water, and the concentration that makes fisetin is 0.75g/100Kg water, then by daily amount of drinking water drinking-water.
Claims (1)
1. the application of fisetin in the preparation medicament for resisting Eimeria tenella.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105625379A CN102028680B (en) | 2010-11-24 | 2010-11-24 | Application of fisetin in preparation of anti-eimeria tenella drugs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105625379A CN102028680B (en) | 2010-11-24 | 2010-11-24 | Application of fisetin in preparation of anti-eimeria tenella drugs |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102028680A CN102028680A (en) | 2011-04-27 |
| CN102028680B true CN102028680B (en) | 2012-05-09 |
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| CN2010105625379A Expired - Fee Related CN102028680B (en) | 2010-11-24 | 2010-11-24 | Application of fisetin in preparation of anti-eimeria tenella drugs |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105380939B (en) * | 2015-11-12 | 2019-04-30 | 广东省农业科学院动物卫生研究所 | Genistein and its medicinal derivative are preparing the application in medicament for resisting Eimeria tenella |
| CN111494362A (en) * | 2020-05-28 | 2020-08-07 | 广东省农业科学院动物卫生研究所 | Application of quercetin and derivatives thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1803195A (en) * | 2005-11-23 | 2006-07-19 | 南京农业大学 | Immunity regulating type Eimeria tenella DNA vaccine for preventing and treating chicken coccidiosis |
| CN101474181A (en) * | 2009-02-18 | 2009-07-08 | 广东省农业科学院兽医研究所 | Application of corytuberine in preparing medicament for resisting Eimeria tenella |
| CN101559051A (en) * | 2004-11-23 | 2009-10-21 | 尚靖 | Use of butin and derivatives thereof for preparing drugs having inhibitive effect on melanoma |
| CN101559050A (en) * | 2004-11-23 | 2009-10-21 | 尚靖 | Use of butin and derivatives thereof for preparing drugs having inhibitive effect on immune system |
| CN101574342A (en) * | 2009-06-12 | 2009-11-11 | 吉林大学 | Application of nitazoxanide in preparing drug for resisting eimeria coccidium |
-
2010
- 2010-11-24 CN CN2010105625379A patent/CN102028680B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101559051A (en) * | 2004-11-23 | 2009-10-21 | 尚靖 | Use of butin and derivatives thereof for preparing drugs having inhibitive effect on melanoma |
| CN101559050A (en) * | 2004-11-23 | 2009-10-21 | 尚靖 | Use of butin and derivatives thereof for preparing drugs having inhibitive effect on immune system |
| CN1803195A (en) * | 2005-11-23 | 2006-07-19 | 南京农业大学 | Immunity regulating type Eimeria tenella DNA vaccine for preventing and treating chicken coccidiosis |
| CN101474181A (en) * | 2009-02-18 | 2009-07-08 | 广东省农业科学院兽医研究所 | Application of corytuberine in preparing medicament for resisting Eimeria tenella |
| CN101574342A (en) * | 2009-06-12 | 2009-11-11 | 吉林大学 | Application of nitazoxanide in preparing drug for resisting eimeria coccidium |
Non-Patent Citations (1)
| Title |
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| 无.常用禽驱虫药用法介绍.《吉林畜牧兽医》.2008,第29卷(第5期),52. * |
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