CN102018687B - Application of hexachlorophene in preparation of anti-eimeria tenella drugs - Google Patents
Application of hexachlorophene in preparation of anti-eimeria tenella drugs Download PDFInfo
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- CN102018687B CN102018687B CN2010105625561A CN201010562556A CN102018687B CN 102018687 B CN102018687 B CN 102018687B CN 2010105625561 A CN2010105625561 A CN 2010105625561A CN 201010562556 A CN201010562556 A CN 201010562556A CN 102018687 B CN102018687 B CN 102018687B
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Abstract
The invention discloses application of hexachlorophene in preparing a medicine for resisting eimeria tenella, wherein hexachlorophene and pharmaceutically acceptable auxiliary materials are uniformly mixed and added into water or feed, and animal infection experiments prove that the anticoccidial index (ACI) of hexachlorophene reaches more than 168.8, and the hexachlorophene has a better effect of resisting eimeria tenella.
Description
Technical field
The present invention relates to a kind of new purposes of hexachlorophene, be specifically related to the application of hexachlorophene in the preparation medicament for resisting Eimeria tenella.
Background technology
Chicken coccidiosis is the main eqpidemic disease that serious harm intensification poultry husbandry is produced, and the therefore annual and economic loss that causes is up to more than 2,500,000,000 pounds, its cause of disease be the former insects of the multiple door in top Eimeria (
Eimeriaspp), wherein harm is maximum be Eimeria tenella (
Eimeria tenella).Though China does not have accurately statistics so far to this, its loss of the most conservative estimation should be not less than 2,500,000,000 yuan of RMB.Chemotherapy is one of important measures of control chicken coccidiosis, but the chicken coccidiosis has extensive and serious Drug resistance at present, presses for new coccidiostat and appears on the market.
Hexachlorophene (Hexachlorophene), chemical name, 2,2 '-methylene two (3,4, the 6-trichlorophenol, 2,4,6,-T), claim the methene trichlorophenol again; Hexachlorophen etc.Being a kind is white in color or the chemical compound of light yellow crystalline powder.The molecular formula of this chemical compound is C
13H
6Cl
6O
2, molecular weight 406.93, CAS accession number: 70-30-4, its chemical constitution is as follows.
Hexachlorophene is to the gram positive bacteria good antimicrobial effect, and to the gram-negative bacteria weak effect, tubercule bacillus and spore do not had effect.Hexachlorophene mainly is used for processing the medicated soap use at present, is used for skin degerming, and the sterilization of wound wound face.
Chicken coccidiosis is the main eqpidemic disease that serious harm intensification poultry husbandry is produced, and the therefore annual and economic loss that causes is up to more than 2,500,000,000 pounds, its cause of disease be the former insects of the multiple door in top Eimeria (
Eimeria spp), wherein harm is maximum be Eimeria tenella (
Eimeria tenella).Though China does not have accurately statistics so far to this, its loss of the most conservative estimation should be not less than 2,500,000,000 yuan of RMB.Chemotherapy is one of important measures of control chicken coccidiosis, appears on the market but the present extensive and serious Drug resistance of chicken coccidiosis presses for new coccidiostat.
So far, do not see the report of the effect of hexachlorophene resisting Eimeria tenella as yet, can't infer through the known character of hexachlorophene whether it has the effect of resisting Eimeria tenella.
Summary of the invention
The object of the present invention is to provide the application of hexachlorophene in the preparation medicament for resisting Eimeria tenella.
The present invention is added in the feedstuff powder and mixing through hexachlorophene and acceptable auxiliary are processed powder afterwards, is mixed with the said hexachlorophene of 15 grams in per 100 kilograms of feedstuffs, by daily forage volume feeding chicken; Or hexachlorophene and acceptable auxiliary processed suspending agent, and suspending agent to be mixed in drinking water, the concentration that makes hexachlorophene is 7.5g/100Kg water, by daily amount of drinking water feeding chicken.Anticoccidial index (ACI) through zoogenetic infection experiment confirm hexachlorophene reaches more than 168.8, has resisting Eimeria tenella effect preferably.
The specific embodiment
Specify the anti-chicken coccidiosis experiment of hexachlorophene below:
Material:
Control drug: by the coccidiostat three word coccidiosis powder (30% sulfaclozine sodium, lot number 001010) of Shanghai Novartis animal health company limited production.
Hexachlorophene: get 30g hexachlorophene (content is not less than 99%) and 70g starch mixing, promptly can be made into powder, said powder is admixed feedstuff, make and be mixed with the said hexachlorophene of 15 grams in per 100 kilograms of feedstuffs; Perhaps get 30g hexachlorophene (content is not less than 99%) and add propylene glycol and stir, fixed dissolving to 100 milliliters can form suspending agent, and described suspending agent is mixed in drinking water, and the concentration that makes hexachlorophene is 7.5g/100Kg water.
Coccidian oocyst: Eimeria tenella (Eimeria tenella) Guangdong strain spore egg capsule, preserve rejuvenation in non-ball worm chickling body before using by the Guangdong Provincial Academy Of Agricultural Sciences Veterinary Institute.
Chickling: the yellow chickling in the south of the Five Ridges, provide by Animal Husbandry Inst., Guangdong Prov. Academy of Agricultural Sciences, raise in the special-purpose Animal House of disinfectant; Chicken coop and the equal strict sterilization of used vessel, free choice feeding and drinking pure water; Observe chickling before the experiment and have or not clinical symptoms and continuous 2d inspection feces to have or not coccidian oocyst, subsequent use.
Feedstuff:, do not contain any coccidiostat by the Xin Nan of Guangdong Province feed corporation,Ltd customization material of brooding all.
Experimental technique:
Divide into groups:Press experiment grouping and processing with 150 south of the Five Ridges Huang breeding chickling to 10 ages in days, pursue and only weigh, young bird and the excessive person of body weight a little less than eliminating will remain chickling and be divided into 5 groups at random, and 30 every group, and suitable the adjustment makes every group of chickling TBW about equally.
Handle:Except that the 1st (blank) group, all the other are respectively organized the equal per os of chickling and irritate clothes Eimeria tenella spore egg capsule l * 10
5Individual/plumage; Except that I group and II (positive control) group, all the other are respectively organized chickling and all after infection, feed immediately or drink feedstuff or the water that contains coccidiostat.
Observation every day and record chicken crowd's mental status, feed intake, feces situation etc.; Dead chickling is weighed, cuts open inspection, if Eimeria tenella infect cause death then lesion score be+4 minutes; All chickling were cutd open inspection by only weighing in the 7th day in the infection back, carried out caecum lesion and kept the score; Calculate the weightening finish and the price of deed of respectively organizing chickling at last.The grouping situation of experiment chickling is seen table 1.
Drug effect decision method and standard anticoccidial index (ACI) are pressed the formula calculating that Merck company is recommended: ACI=(the relative weight gain rate+survival rate) (egg capsule value+pathological changes value); The relative weight gain rate=(weightening finish of experimental group weightening finish ÷ blank group) * 100%; Survival rate=(each group survival chickling is counted ÷ and respectively organizes the chickling sum) * 100%.Pathological changes is kept the score by Pyatyi: 1. do not have egg capsule, caecum is normal, is 0 minute; 2. egg capsule is arranged, and the caecum mucosa thickens slightly, has to be dispersed in hemorrhage or a small amount of blood sample intestinal contents on a small quantity, is+1 fen; 3. egg capsule is arranged, and the caecum mucosa thickens, and obviously hemorrhage or obvious blood sample intestinal contents is arranged, and is+2 fens; 4. egg capsule is arranged, and the caecum mucosa thickens, and a large amount of sludged bloods or blood sample intestinal core are arranged, and is+3 fens; 5. chickling is dead or have a large amount of egg capsules, caecum outward appearance to be color of soy sauce (or small intestinal middle part the point-like necrosis region is arranged, the mucosa face is blush) because of coccidiosis, the obvious enlargement of intestinal tube, and content forms tangible blood sample intestinal core, is+4 fens.Pathological changes value in the group=every group of average lesion score * 20.Drug effect criterion is that < 120 for invalid, and 120-160 is weak effect, and 160-180 is middle effect, is strong the effect more than 180 for ACI.The egg capsule value restrains egg sac number (OPG) by cecal content and converts.
Experimental result:
Clinicing symptom observation:
Chickling in the matched group of inoculation Eimeria tenella spore egg capsule, it is depressed spirit to occur at the 2nd day, slow-witted upright, loss of appetite; Occurred obvious hemafecia on the 5th day, and death occurs, hemafecia reduces gradually subsequently.
The anticoccidial index:
The result sees shown in the table 2
Example 1
The preparation of powder: get 30g hexachlorophene (content is not less than 99%) and 70g starch mixing, get final product.
Usage and dosage: make to be mixed with the said hexachlorophene of 15 grams in per 100 kilograms of feedstuffs, by daily appetite feeding.
Example 2
The preparation of suspending agent: get 30g hexachlorophene (content is not less than 99%) and add propylene glycol and stir, fixed dissolving to 100 milliliters gets final product.
Usage and dosage: described suspending agent mixes in drinking water, and the concentration that makes hexachlorophene is 7.5g/100Kg water, then by daily amount of drinking water drinking-water.
Claims (1)
1. the application of hexachlorophene in the preparation medicament for resisting Eimeria tenella.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105625561A CN102018687B (en) | 2010-11-24 | 2010-11-24 | Application of hexachlorophene in preparation of anti-eimeria tenella drugs |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105625561A CN102018687B (en) | 2010-11-24 | 2010-11-24 | Application of hexachlorophene in preparation of anti-eimeria tenella drugs |
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| Publication Number | Publication Date |
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| CN102018687A CN102018687A (en) | 2011-04-20 |
| CN102018687B true CN102018687B (en) | 2012-05-09 |
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| CN103565783B (en) * | 2012-08-09 | 2016-02-24 | 中国科学院上海药物研究所 | The application in voltage gated k+ channel blocker agonist prepared by hexachlorophene |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1803195A (en) * | 2005-11-23 | 2006-07-19 | 南京农业大学 | Immunity regulating type Eimeria tenella DNA vaccine for preventing and treating chicken coccidiosis |
| CN101474181A (en) * | 2009-02-18 | 2009-07-08 | 广东省农业科学院兽医研究所 | Application of corytuberine in preparing medicament for resisting Eimeria tenella |
| CN101574342A (en) * | 2009-06-12 | 2009-11-11 | 吉林大学 | Application of nitazoxanide in preparing drug for resisting eimeria coccidium |
-
2010
- 2010-11-24 CN CN2010105625561A patent/CN102018687B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1803195A (en) * | 2005-11-23 | 2006-07-19 | 南京农业大学 | Immunity regulating type Eimeria tenella DNA vaccine for preventing and treating chicken coccidiosis |
| CN101474181A (en) * | 2009-02-18 | 2009-07-08 | 广东省农业科学院兽医研究所 | Application of corytuberine in preparing medicament for resisting Eimeria tenella |
| CN101574342A (en) * | 2009-06-12 | 2009-11-11 | 吉林大学 | Application of nitazoxanide in preparing drug for resisting eimeria coccidium |
Non-Patent Citations (2)
| Title |
|---|
| 无.常用禽驱虫药用法介绍.《吉林畜牧兽医》.2008,第29卷(第5期),52. * |
| 王志军.柔嫩艾美耳球虫β-酮脂酰-ACP还原酶基因的克隆表达和酶动力学研究.《中国优秀硕士学位论文全文数据库》.2009,72-73. * |
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