CN102028680A - Application of fisetin for resisting against Eimeria tenella - Google Patents
Application of fisetin for resisting against Eimeria tenella Download PDFInfo
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- CN102028680A CN102028680A CN 201010562537 CN201010562537A CN102028680A CN 102028680 A CN102028680 A CN 102028680A CN 201010562537 CN201010562537 CN 201010562537 CN 201010562537 A CN201010562537 A CN 201010562537A CN 102028680 A CN102028680 A CN 102028680A
- Authority
- CN
- China
- Prior art keywords
- fisetin
- eimeria tenella
- chickling
- application
- resisting against
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- XHEFDIBZLJXQHF-UHFFFAOYSA-N fisetin Chemical compound C=1C(O)=CC=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 XHEFDIBZLJXQHF-UHFFFAOYSA-N 0.000 title claims abstract description 53
- 235000011990 fisetin Nutrition 0.000 title claims abstract description 26
- 241000223932 Eimeria tenella Species 0.000 title claims abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 6
- 239000000463 material Substances 0.000 abstract description 3
- 239000000203 mixture Substances 0.000 abstract description 2
- 239000002775 capsule Substances 0.000 description 10
- 241000252983 Caecum Species 0.000 description 6
- 208000003495 Coccidiosis Diseases 0.000 description 6
- 206010023076 Isosporiasis Diseases 0.000 description 6
- 210000004534 cecum Anatomy 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 241000287828 Gallus gallus Species 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 5
- 230000001165 anti-coccidial effect Effects 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 239000003224 coccidiostatic agent Substances 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 230000000968 intestinal effect Effects 0.000 description 4
- 210000004877 mucosa Anatomy 0.000 description 4
- 239000000843 powder Substances 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 230000002354 daily effect Effects 0.000 description 3
- 239000003651 drinking water Substances 0.000 description 3
- 235000020188 drinking water Nutrition 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 230000003902 lesion Effects 0.000 description 3
- 231100000915 pathological change Toxicity 0.000 description 3
- 230000036285 pathological change Effects 0.000 description 3
- 210000004215 spore Anatomy 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 239000000375 suspending agent Substances 0.000 description 3
- 241000224483 Coccidia Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 208000032843 Hemorrhage Diseases 0.000 description 2
- MJBPUQUGJNAPAZ-AWEZNQCLSA-N butin Chemical compound C1([C@@H]2CC(=O)C3=CC=C(C=C3O2)O)=CC=C(O)C(O)=C1 MJBPUQUGJNAPAZ-AWEZNQCLSA-N 0.000 description 2
- 230000034994 death Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000857 drug effect Effects 0.000 description 2
- 210000003608 fece Anatomy 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 210000003736 gastrointestinal content Anatomy 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 210000003250 oocyst Anatomy 0.000 description 2
- XQYZDYMELSJDRZ-UHFFFAOYSA-N papaverine Chemical compound C1=C(OC)C(OC)=CC=C1CC1=NC=CC2=CC(OC)=C(OC)C=C12 XQYZDYMELSJDRZ-UHFFFAOYSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 230000004584 weight gain Effects 0.000 description 2
- 235000019786 weight gain Nutrition 0.000 description 2
- 229930008281 A03AD01 - Papaverine Natural products 0.000 description 1
- 241000208223 Anacardiaceae Species 0.000 description 1
- MJBPUQUGJNAPAZ-UHFFFAOYSA-N Butine Natural products O1C2=CC(O)=CC=C2C(=O)CC1C1=CC=C(O)C(O)=C1 MJBPUQUGJNAPAZ-UHFFFAOYSA-N 0.000 description 1
- 206010010904 Convulsion Diseases 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000223924 Eimeria Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- PPQNQXQZIWHJRB-UHFFFAOYSA-N Methylcholanthrene Chemical compound C1=CC=C2C3=CC4=CC=C(C)C(CC5)=C4C5=C3C=CC2=C1 PPQNQXQZIWHJRB-UHFFFAOYSA-N 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 244000044283 Toxicodendron succedaneum Species 0.000 description 1
- 230000001004 anti-acetylcholinic effect Effects 0.000 description 1
- 230000004596 appetite loss Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 239000000812 cholinergic antagonist Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000036461 convulsion Effects 0.000 description 1
- 210000000695 crystalline len Anatomy 0.000 description 1
- 230000000994 depressogenic effect Effects 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 235000021050 feed intake Nutrition 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 238000003304 gavage Methods 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 235000021266 loss of appetite Nutrition 0.000 description 1
- 208000019017 loss of appetite Diseases 0.000 description 1
- 230000003340 mental effect Effects 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 229960001789 papaverine Drugs 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 150000003180 prostaglandins Chemical class 0.000 description 1
- 201000007094 prostatitis Diseases 0.000 description 1
- 230000003716 rejuvenation Effects 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- NIUFFODHMKLSCH-UHFFFAOYSA-N sodium;(4-aminophenyl)sulfonyl-(6-chloropyrazin-2-yl)azanide Chemical compound [Na+].C1=CC(N)=CC=C1S(=O)(=O)[N-]C1=CN=CC(Cl)=N1 NIUFFODHMKLSCH-UHFFFAOYSA-N 0.000 description 1
- 235000013555 soy sauce Nutrition 0.000 description 1
- 230000002048 spasmolytic effect Effects 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Landscapes
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention discloses an application of fisetin for resisting against Eimeria tenella. Fisetin is uniformly mixed with acceptable auxiliary materials, and the mixture can be added to water or feed for resisting against Eimeria tenella effectively.
Description
Technical field
The present invention relates to a kind of new purposes of fisetin, particularly fisetin is in the application of resisting Eimeria tenella.
Background technology
Chicken coccidiosis is the main eqpidemic disease that serious harm intensification poultry husbandry is produced, therefore the economic loss that cause every year is up to more than 2,500,000,000 pounds, its cause of disease is the Eimeria (Eimeriaspp) of the former insects of the multiple door in top, and that wherein harm is maximum is Eimeria tenella (Eimeria tenella).Though China does not have accurately statistics so far to this, its loss of the most conservative estimation should be not less than 2,500,000,000 yuan of RMB.Chemotherapy is one of important measures of control chicken coccidiosis, but the chicken coccidiosis has extensive and serious Drug resistance at present, presses for new coccidiostat and appears on the market.
Fisetin (Fisetin), chemical name, 3,3 ', 4 ', the 7-kaempferol claims fisetin again; Butin; Fisetin etc.Can be from the cured tree Rhus succedanea L of Anacardiaceae plant wood.Extract in the plants such as timber.The molecular formula of this chemical compound is C15H10O6, molecular weight 286.23, and CAS accession number: 528-48-3, its chemical constitution is suc as formula shown in (I).
The main pharmacological of existing reported in literature has:
1, suppresses prostaglandin: to fibrocellular prostatitis by the 3T3 mice of methyl cholanthrene transformation
The generation of gland rope has inhibitory action, and half effective inhibition concentration (ID50) is 42.0UM.
2, enzyme suppresses: the complete sugared reductase to the rat crystalline lens has inhibitory action, and half effective inhibition concentration is 10-6M
3, spasmolytic: to the mouse small intestine specimen, its effectiveness that causes the convulsion effect to anti-acetylcholine is 166% of papaverine.
4, a group by Japan, American Studies composition of personnel finds that through zoopery the fisetin in vegetable and the fruit helps improving memory
In addition, also have the report of fisetin as biological dye.
Up to now, there is not fisetin to have the relevant report of resisting Eimeria tenella effect, can't infers by the known character of fisetin whether it has the effect of resisting Eimeria tenella.
Summary of the invention
The object of the present invention is to provide the application of fisetin at resisting Eimeria tenella.
Fisetin and acceptable auxiliary are made preparation, be added in feedstuff or the water, by daily method feeding chickling.Zoogenetic infection experiment confirm fisetin has good resisting Eimeria tenella effect, and its anticoccidial index (ACI) can reach more than 150..
The specific embodiment
The anti-chicken coccidiosis experiment of fisetin
Experiment material
Control drug: conventional coccidiostat three word coccidiosis powder (30% sulfaclozine sodium, lot number 001010), produce by Shanghai Novartis animal health company limited;
Fisetin: be mixed with the 2g fisetin in 100 kilograms of feedstuffs, mixing, perhaps dissolving 0.75g fisetin in 100kg water shakes up;
Coccidian oocyst: Eimeria tenella (Eimeria tenella) Guangdong strain spore egg capsule, preserve rejuvenation in non-ball worm chickling body before using by the Guangdong Provincial Academy Of Agricultural Sciences Veterinary Institute;
Chickling: the yellow chickling in the south of the Five Ridges, provide by Animal Husbandry Inst., Guangdong Prov. Academy of Agricultural Sciences, raise in the special-purpose Animal House of disinfectant; Chicken coop and the equal strict sterilization of used vessel, free choice feeding and drinking pure water; Observe chickling before the test and have or not clinical symptoms and continuous 2d to check that feces has or not coccidian oocyst, standby.
Feedstuff:, do not contain any coccidiostat by the Xin Nan of Guangdong Province feed corporation,Ltd customization material of brooding all.
Experimental technique
Grouping: press test grouping and processing with yellow breeding chickling to 10 age in days in 150 south of the Five Ridges, pursue and only weigh, eliminate weak young bird and the excessive person of body weight, will remain chickling and be divided into 5 groups at random, 30 every group, and suitably adjustment makes every group of chickling TBW about equally;
Handle: except that the 1st (blank) group, all the other are respectively organized the equal per os of chickling and gavage Eimeria tenella spore egg capsule 1 * 10
5Individual/plumage; Except that I group and II (positive control) group, all the other are respectively organized chickling and all feed immediately after infection or drink feedstuff or the water that contains coccidiostat.
Observation every day and record chicken group's mental status, feed intake, feces situation etc.; Dead chickling is weighed, cuts open inspection, if Eimeria tenella infect cause death then lesion score be+4 minutes; All chickling were cutd open inspection by only weighing in the 7th day in the infection back, carried out caecum lesion and kept the score; Calculate the weightening finish and the price of deed of respectively organizing chickling at last.The grouping situation of test chickling sees Table 1.
The grouping of table 1 test chickling
Drug effect decision method and standard anticoccidial index (ACI) are pressed the formula calculating that Merck company is recommended: ACI=(the relative weight gain rate+survival rate) (egg capsule value+pathological changes value); The relative weight gain rate=(weightening finish of test group weightening finish ÷ blank group) * 100%; Survival rate=(each group survival chickling is counted ÷ and respectively organizes the chickling sum) * 100%.Pathological changes is kept the score by Pyatyi: 1. do not have egg capsule, caecum is normal, is 0 minute; 2. egg capsule is arranged, and the caecum mucosa thickens slightly, has to be dispersed in hemorrhage or a small amount of blood sample intestinal contents on a small quantity, is+1 fen; 3. egg capsule is arranged, and the caecum mucosa thickens, and obviously hemorrhage or obvious blood sample intestinal contents is arranged, and is+2 fens; 4. egg capsule is arranged, and the caecum mucosa thickens, and a large amount of sludged bloods or blood sample intestinal core are arranged, and is+3 fens; 5. chickling is dead or have a large amount of egg capsules, caecum outward appearance to be color of soy sauce (or small intestinal middle part the point-like necrosis region is arranged, the mucosa face is blush) because of coccidiosis, the obvious enlargement of intestinal tube, and content forms tangible blood sample intestinal core, is+4 fens.Pathological changes value in the group=every group of average lesion score * 20.Drug effect criterion is that ACI＜120 are invalid, and 120-160 is weak effect, and 160--180 is middle effect, is potent more than 180.The egg capsule value restrains egg sac number (OPG) by cecal content and converts.
Experimental result:
Clinicing symptom observation
Chickling in the matched group of inoculation Eimeria tenella spore egg capsule, it is depressed spirit to occur at the 2nd day, slow-witted upright, loss of appetite; Occurred obvious hemafecia on the 5th day, and death occurs, hemafecia reduces gradually subsequently.
Calculate the anticoccidial index of each group respectively, the results are shown in Table shown in 2.
Table 2 is respectively organized the anticoccidial index
Example 1
Powder: get 15g fisetin (content is not less than 99%) and 80g starch mixing, get final product.
The usage of powder: described powder is admixed feedstuff, make to be mixed with the described fisetin of 1.5 grams in per 100 kilograms of feedstuffs, then by daily forage volume feeding.
Example 2
Suspending agent: get 15g fisetin (content is not less than 99%) and add the ethanol stirring, be dissolved in 100 milliliters surely and get final product.
The suspending agent usage: described suspending agent is mixed in drinking water, and the concentration that makes fisetin is 0.75g/100Kg water, then by daily amount of drinking water drinking-water.
Claims (1)
1. fisetin is in the application of resisting Eimeria tenella.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105625379A CN102028680B (en) | 2010-11-24 | 2010-11-24 | Application of fisetin in preparation of anti-eimeria tenella drugs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105625379A CN102028680B (en) | 2010-11-24 | 2010-11-24 | Application of fisetin in preparation of anti-eimeria tenella drugs |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102028680A true CN102028680A (en) | 2011-04-27 |
| CN102028680B CN102028680B (en) | 2012-05-09 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010105625379A Expired - Fee Related CN102028680B (en) | 2010-11-24 | 2010-11-24 | Application of fisetin in preparation of anti-eimeria tenella drugs |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102028680B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105380939A (en) * | 2015-11-12 | 2016-03-09 | 广东省农业科学院动物卫生研究所 | Genistein and application of medicinal derivative thereof in preparation of medicine for resisting eimeria tenella |
| CN111494362A (en) * | 2020-05-28 | 2020-08-07 | 广东省农业科学院动物卫生研究所 | Application of quercetin and derivatives thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1803195A (en) * | 2005-11-23 | 2006-07-19 | 南京农业大学 | Immunity regulating type Eimeria tenella DNA vaccine for preventing and treating chicken coccidiosis |
| CN101474181A (en) * | 2009-02-18 | 2009-07-08 | 广东省农业科学院兽医研究所 | Application of corytuberine in preparing medicament for resisting Eimeria tenella |
| CN101559051A (en) * | 2004-11-23 | 2009-10-21 | 尚靖 | Use of butin and derivatives thereof for preparing drugs having inhibitive effect on melanoma |
| CN101559050A (en) * | 2004-11-23 | 2009-10-21 | 尚靖 | Use of butin and derivatives thereof for preparing drugs having inhibitive effect on immune system |
| CN101574342A (en) * | 2009-06-12 | 2009-11-11 | 吉林大学 | Application of nitazoxanide in preparing drug for resisting eimeria coccidium |
-
2010
- 2010-11-24 CN CN2010105625379A patent/CN102028680B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101559051A (en) * | 2004-11-23 | 2009-10-21 | 尚靖 | Use of butin and derivatives thereof for preparing drugs having inhibitive effect on melanoma |
| CN101559050A (en) * | 2004-11-23 | 2009-10-21 | 尚靖 | Use of butin and derivatives thereof for preparing drugs having inhibitive effect on immune system |
| CN1803195A (en) * | 2005-11-23 | 2006-07-19 | 南京农业大学 | Immunity regulating type Eimeria tenella DNA vaccine for preventing and treating chicken coccidiosis |
| CN101474181A (en) * | 2009-02-18 | 2009-07-08 | 广东省农业科学院兽医研究所 | Application of corytuberine in preparing medicament for resisting Eimeria tenella |
| CN101574342A (en) * | 2009-06-12 | 2009-11-11 | 吉林大学 | Application of nitazoxanide in preparing drug for resisting eimeria coccidium |
Non-Patent Citations (1)
| Title |
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| 《吉林畜牧兽医》 20081231 无 常用禽驱虫药用法介绍 52 1 第29卷, 第5期 2 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105380939A (en) * | 2015-11-12 | 2016-03-09 | 广东省农业科学院动物卫生研究所 | Genistein and application of medicinal derivative thereof in preparation of medicine for resisting eimeria tenella |
| CN105380939B (en) * | 2015-11-12 | 2019-04-30 | 广东省农业科学院动物卫生研究所 | Genistein and its medicinal derivative are preparing the application in medicament for resisting Eimeria tenella |
| CN111494362A (en) * | 2020-05-28 | 2020-08-07 | 广东省农业科学院动物卫生研究所 | Application of quercetin and derivatives thereof |
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| Publication number | Publication date |
|---|---|
| CN102028680B (en) | 2012-05-09 |
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