CN101926452B - Wild jujube leaf total flavone extract and preparation method thereof - Google Patents

Abstract

A wild jujube leaf flavone extract is prepared through extracting concentrated general flavone extract from wild jujube leaf by alcohol extraction method, using alcohol solvent with a certain concentration, optimizing extraction time, extraction temp. and ratio of raw materials to liquid to obtain optimum extraction condition, preparing jujube leaf flavone extract liquid, filtering and concentrating. The crude extract has yield of more than 60%, and is rich in various nutritional components, such as total flavone content of more than 9%, protein content of more than 5%, total sugar content of more than 4%, and tannin content of 1%. The invention has simple preparation process, convenient operation and suitability for large-scale industrial production.

Description

Sour jujube leaf total flavonoids extract and preparation method thereof

technical field

The invention relates to an extraction process for preparing flavonoid extract from wild jujube leaves, which belongs to the field of food science and technology. the

Background technique

Flavonoids are a class of secondary metabolites that widely exist in higher plants. They have a strong ability to scavenge free radicals and are one of the main active ingredients of medicinal plants. Relevant pharmacological and clinical experiments have confirmed that flavonoids have a wide range of For example, many Chinese herbal medicines that are effective in treating coronary heart disease or traditional Chinese medicines that promote blood circulation and remove blood stasis contain flavonoids. Since this kind of compound has a variety of biological activities and pharmacological functions, and has low toxicity, it has attracted people's attention and has become the focus of research, development and utilization of functional foods. Flavonoids are widely found in fruits, flowers, and leaves of plants, and leaves have a large amount in nature and a wide range of sources. Therefore, the extraction of flavonoids from plant leaves has been widely developed in recent years. For example, the publication number is CN 101224224A , Application No. 200810010012.7 "Method for Extracting Crude Flavonoids and Flavone Glycosides from Ginkgo Leaf", which uses Ginkgo biloba as raw material to extract flavonoids; The leaves are used as raw materials to extract flavonoids, but there is no report on the extraction of flavonoids from jujube leaves. the

Sour jujube, also known as wild jujube, is a perennial shrub that grows in northern my country. There is a large area of wild jujube forest in northern my country, and the resources are very rich. The flowers, fruits, branches, leaves and kernels of Zizyphus jujuba have great pharmacological nutritional value. At present, the research on the flavonoids of Zizyphus jujuba is relatively sufficient, but the development and utilization of jujube leaves is still in a blank stage. the

The present invention uses a solvent to extract and measure the total flavonoids in spiny jujube leaves, aiming to find a suitable method for extracting the total flavonoids in spiny jujube leaves, and to provide a scientific and effective preparation process for the deep development and utilization of spiny jujube products, which is also convenient for large-scale production. The comprehensive development and utilization of mountain resources provides a new way. the

Contents of the invention

The purpose of the present invention is to utilize wild spiny jujube leaves and optimize extraction conditions to obtain a higher content of total flavonoid extract from spiny jujube leaves, and to open up a broader market prospect for the industrial development of spiny jujube related products. the

See the accompanying drawings for the process flow chart, and the specific process is as follows:

(1) Dried jujube leaves and crushed

Take the wild jujube leaves, fix them at 105°C for 15 minutes, ventilate and dry them at 65°C until they reach a constant weight, and then pulverize them. the

(2) Preparation of defatted jujube leaf powder

The dried and pulverized spiny jujube leaf powder is degreased by reflux leaching with 5 times the volume of petroleum ether for 1.5 h, and the defatted spiny jujube leaf powder is desolventized to obtain defatted spiny jujube leaf powder. the

(2) Preparation of Crude Extract of Jujube Leaves

A certain amount of defatted jujube leaf powder is weighed, extracted with an ethanol solvent having a volume ratio of 50-80% for 1-3 hours, the extraction temperature is 50-80° C., and the ratio of solid to liquid is 1:30-1:60. After the extraction is completed, filter and discard the precipitate, combine the extracts, and concentrate under reduced pressure under vacuum at a temperature lower than 60°C. When the soluble solids in the extract reach 40%, a paste-like concentrate is obtained (it can also be processed The powdery crude product was obtained after vacuum drying). In addition, macroporous adsorption resin can also be used for refining to obtain jujube leaf flavonoid products with higher purity. the

(3) Main technical indicators of the product

The product is a brownish-yellow paste, slightly bitter. Extraction rate of total flavonoids from jujube leaves is ≥60%, total flavonoid content is ≥9%, total flavonoid extraction rate is ≥78%, protein content is ≥5%, total sugar content is ≥4%, and tannin content is 1%. the

(4) Detection method

Alkaline NaNO ₂ -Al(NO ₃ ) ₃ chromogenic method was used to measure the absorbance of the samples at a wavelength of 510nm with rutin as a standard, and the total flavonoid content of jujube leaves was calculated according to the standard curve.

Detailed ways

The present invention is further described in detail by the following examples, which are illustrative, not restrictive, and the protection scope of the present invention cannot be limited by the following examples. the

Grinding the dried jujube leaves into powder, reflux extracting with 5 times the volume of petroleum ether for degreasing for 1.5 h, and desolventizing the degreased jujube leaf powder to obtain defatted jujube leaf powder. Weigh the quantitatively dried defatted jujube leaf powder, add 60% (volume ratio) ethanol solution, and extract in a water bath at 67° C. for 1.5 h, and the amount of ethanol is a solid-liquid ratio of 1:50. After the extraction is completed, filter and collect the combined filtrate. Centrifuge the extract of jujube leaves above to remove impurities, and collect the supernatant for later use. The supernatant was concentrated under reduced pressure in vacuo to recover ethanol. The concentration temperature is 60°C, the vacuum degree is -0.1～-0.06MPa, until the soluble solid content of the concentrated solution reaches 40%. (The paste concentrate can also be freeze-dried and pulverized to make the crude extract of total flavonoids from Suanzao leaves). The yield rate is 61.5%. The content of total flavonoids in jujube leaves in the paste is 9.1%, protein is 5.5%, total sugar is 4.3%, and tannin is 1%. the

Claims (1)

1. Folium Ziziphi Spinosae flavones extract making method is characterized in that comprising following preparation process:

(1) gets the Wild jujube leaf and complete, 80-120 ℃ of the temperature that completes, fixation time 10-30min; After 65 ℃ of following air seasoning to constant weights, pulverize, degreasing, degreasing solvent is sherwood oil or solvent oil; Folium Ziziphi Spinosae powder after the degreasing is carried out desolventizing, obtain degreasing Folium Ziziphi Spinosae powder;

(2) with 50-80% ethanol (volume ratio) lixiviate step (1) Folium Ziziphi Spinosae powder; Extract the pure soluble components in the Folium Ziziphi Spinosae; Obtain extracting solution, extraction time is 1-3h, extraction temperature 50-80 ℃; Solid-liquid ratio is 1: 30-1: 60, and any method in percolation, single-stage pickling process, multistage pickling process, continuous countercurrent extraction and the circumfluence method is taked in lixiviate;

(3) extracting solution that obtains of step (2) concentrates and reclaims ethanol and process Folium Ziziphi Spinosae flavones medicinal extract, wherein general flavone content >=9%.

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